CN114395479A - Stem cell biological growth observation device for rectal adenocarcinoma treatment - Google Patents
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Abstract
The invention relates to the field of cell culture observation, in particular to a stem cell biological growth observation device for rectal adenocarcinoma treatment, which comprises an incubator, a cell observation device and a supply pipe device arranged in a rotating manner, wherein the supply pipe device can supply hot air, cold air and water mist into the incubator, a plurality of culture dishes are arranged in the incubator, the culture dishes are uniformly distributed around the outer side of the supply pipe device, are all connected with the supply pipe device, the supply pipe device is connected with a driving device, the driving device can drive a rotating pipe part of the supply pipe device to drive the culture dishes to rotate, a first through hole is formed in the incubator and used for the culture dishes to come in and go out, a door structure capable of opening and closing the first through hole is arranged at the outer end of the first through hole, and the cell observation device can observe cells in the culture dishes positioned in the first through hole.
Description
Technical Field
The invention relates to the field of cell culture observation, in particular to a stem cell biological growth observation device for rectal adenocarcinoma treatment.
Background
Rectal adenocarcinoma belongs to one kind of rectal cancer, which is adenocarcinoma from a dentate line to a position between a sigmoid colon and a rectal transitional part, accounts for 75-85% of the colorectal cancer, and is a common malignant tumor of a digestive tract, but the etiology is still unclear so far, and in order to research the etiology, a series of growth observation experiments need to be carried out on rectal adenocarcinoma cells; the time that the two other culture dishes are exposed to the outside when waiting for observation is too long, which is easy to cause unpredictable change; thirdly, the existing incubator has poor functionality, can only realize simple heating and refrigerating functions, and the heating or refrigerating element is firstly used for locally heating or refrigerating, so that cells in the incubator cannot be uniformly heated or cooled; therefore, the device for observing the growth of the rectal adenocarcinoma cells is designed aiming at the defects.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides a stem cell biological growth observation device for rectal adenocarcinoma treatment.
The technical scheme of the invention is as follows:
the invention provides a stem cell biological growth observation device for treating rectal adenocarcinoma, which comprises an incubator, a cell observation device positioned on the upper side of the incubator, and a supply pipe device rotatably arranged in the middle of the incubator, wherein the supply pipe device can supply hot air, cold air and water mist into the incubator, a plurality of culture dishes are arranged in the incubator, the culture dishes are uniformly distributed around the outer side of the supply pipe device, the culture dishes are all connected with the supply pipe device, the supply pipe device is connected with a driving device, the driving device can drive a rotating pipe part of the supply pipe device to drive the culture dishes to rotate, a first through hole is formed in the incubator and used for the culture dishes to come in and go out, a door structure capable of opening and closing the first through hole is arranged at the outer end of the first through hole, and the cell observation device can observe cells in the culture dishes positioned in the first through hole.
Further, the cell observation apparatus is a microscope.
Furthermore, the supply pipe device comprises a first vertical pipe and a second vertical pipe, the first vertical pipe is rotatably arranged, the second vertical pipe is fixedly arranged in the first vertical pipe, the outer wall of the second vertical pipe is in contact fit with the inner wall of the first vertical pipe, the first vertical pipe is connected with the culture dish through a connecting rod, the first vertical pipe is connected with a driving device, the driving device can drive the first vertical pipe to rotate, a plurality of second through holes are formed in the outer side of the first vertical pipe, third through holes are formed in the outer side of the second vertical pipe corresponding to the second through holes one by one, the second through holes can be controlled to be aligned with and staggered with the third through holes through rotation of the first vertical pipe, one end of the first vertical pipe is connected with the cold air supply device, the other end of the first vertical pipe is connected with the water mist hot air supply device, and the outer side of the second vertical pipe is connected with blades.
Furthermore, the outer ends of the second through holes are provided with water mist nozzles.
Furthermore, water smoke steam feeding mechanism includes the water tank, and the water tank is installed to the bottom of incubator, the bottom of water tank with hold the case parallel and level, install heating module, water pump in the water tank, the lower extreme of second standpipe links to each other with the water pump, a plurality of inlet ports have been seted up to the lower part of second standpipe, the lower extreme of second standpipe is equipped with second one way solenoid valve, second one way solenoid valve is located the upside of inlet port, the upper portion of first standpipe is equipped with first one way solenoid valve, the upper end of first standpipe links to each other with air conditioning feeding mechanism.
Furthermore, a pressure release valve is arranged on the incubator.
Furthermore, a plurality of connecting rods are annularly and uniformly connected to the outer side of the first vertical pipe, limiting rings are connected to the outer ends of the connecting rods, a culture dish is arranged on the upper side of the limiting rings, an annular groove is formed in the bottom of the culture dish corresponding to the limiting rings, the limiting rings can be inserted into the annular groove, the culture dishes are annularly and uniformly distributed on the outer side of the first vertical pipe, an elastic sealing ring is arranged in each first through hole, the culture dish can be in sealing contact with the elastic sealing ring in a matching mode, a door structure capable of being opened and closed is arranged at the outer end of each first through hole, the door structure can completely seal the first through holes, a plurality of limiting blocks are annularly and uniformly distributed at the bottom of the culture dish around the center of the bottom of the culture dish, the limiting blocks are positioned on the outer side of the first magnetic plate, a second lifting device is arranged on the lower side of the limiting rings, the movable end of the second lifting device can penetrate through the limiting rings, the bottom of the culture dish is connected with a first magnetic plate, and a second magnetic plate is arranged at the movable end of the second lifting device, the second magnetic plate can be attracted to the first magnetic plate.
Furthermore, the second lifting device is an electric telescopic rod, and a movable rod of the electric telescopic rod upwards penetrates through the side wall of the incubator and is in contact fit with the side wall.
Furthermore, the bottom installation of incubator holds the case, holds the incasement and is equipped with electric telescopic handle's fixed part.
Furthermore, the cell observation device is connected with the incubator through a first lifting device.
The invention achieves the following beneficial effects:
the culture box is simple in structure and ingenious in design, the second through hole and the third through hole can be conveniently controlled to be aligned or staggered through rotation of the first vertical pipe, and cold air, hot air and water mist can be supplied or a sealed environment in the culture box can be maintained respectively; the culture boxes are supplied with cold air, hot air and water mist through the pipelines positioned in the centers among the culture dishes, so that the good regulation and control of the temperature and the humidity in the culture boxes are realized, meanwhile, the first vertical pipes can drive the culture dishes to rotate at a constant speed relative to the middle spray outlets, so that the cold air, the hot air and the water mist can be uniformly diffused in the culture boxes, the environment of the rectal adenocarcinoma cells in the culture dishes can be kept in a single variable, and the accuracy of test results can be conveniently compared; first standpipe can drive the culture dish and rotate to the assigned position for second elevating gear can make the culture dish jack-up be convenient for the user to carry out clear observation to the rectum adenocarcinoma cell in it through the microscope, simultaneously through the elastic sealing circle, the door structure, cooperation between first and the second magnetic plate can be accomplished and realize observing the cell in the culture dish under the condition that need not open the incubator completely with external intercommunication, the contaminated possibility of culture medium in the culture dish has been reduced, the good environment of cell normal growth has been guaranteed.
Drawings
FIG. 1 is a schematic overall structure of one embodiment of the present invention;
FIG. 2 is a schematic structural view of the view from the A direction of FIG. 1;
FIG. 3 is an enlarged view of I of FIG. 1;
FIG. 4 is a schematic view of the connection between the culture dish and the first standpipe;
FIG. 5 is an enlarged view of II of FIG. 3;
fig. 6 is an enlarged view of iii of fig. 3.
Detailed Description
To facilitate an understanding of the present invention by those skilled in the art, specific embodiments thereof are described below with reference to the accompanying drawings.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present application, it is to be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
It will be understood that when an element is referred to as being "mounted on" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. When an element is referred to as being "secured to" another element, it can be directly secured to the other element or intervening elements may also be present.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "or/and" includes any and all combinations of one or more of the associated listed items.
As shown in figures 1-6, the invention provides a stem cell biological growth observation device for rectal adenocarcinoma treatment, which comprises an incubator 1, wherein a first lifting device 12 is installed at the top of the incubator 1, the movable end of the first lifting device 12 is connected with a microscope 13, the middle part of the incubator 1 penetrates through and is rotatably connected with a first vertical pipe 2, the upper end of the first vertical pipe 2 upwards penetrates through the incubator 1, a plurality of connecting rods 3 positioned inside the incubator 1 are annularly and uniformly welded at the outer side of the first vertical pipe 2, limiting rings 3 are welded at the outer ends of the connecting rods 3, a culture dish 4 is placed on the upper side of the limiting rings 3, an annular groove 5 is formed in the bottom of the culture dish 4 corresponding to the limiting rings, the limiting rings 3 can be inserted into the annular groove 5, the limiting rings 4 play a role in limiting and fixing the culture dish 4 after being inserted into the annular groove 5, the culture dishes 4 are annularly and uniformly distributed at the outer side of the first vertical pipe 2, the first vertical pipe 2 can drive the culture dish 4 to rotate together when rotating, a first through hole 7 is arranged at the top of the culture box 1 far away from the center corresponding to the culture dish 4, the first through hole 7 is positioned at the lower side of the lens of the microscope 13, the culture dish 4 can pass through the first through hole 7, an elastic sealing ring 8 is arranged in the first through hole 7, the culture dish 4 can be in sealing contact fit with the elastic sealing ring 8, a door structure 9 capable of being opened and closed is arranged at the outer end of the first through hole 7, the door structure 9 can completely seal the first through hole 7, the door structure 9 can ensure the sealing condition of the culture box at ordinary times and play the roles of dust prevention and pollution prevention, when cells in the culture dish need to be observed, the culture dish is in sealing contact fit with the elastic sealing ring, the door structure is opened, thereby enabling the user to observe the cells in the culture dish through the microscope without communicating the inside of the incubator with the outside.
The bottom of the culture dish 4 is uniformly and annularly connected with a plurality of limiting blocks 6 around the center of the culture dish, the limiting blocks 6 are positioned on the outer side of the first magnetic plate 30, when the culture dish needs to be taken out for use, the limiting blocks 6 can be used as supporting legs to support the culture dish 4, the lower side of the limiting ring 3 is provided with a second lifting device 10, the second lifting device 10 comprises but is not limited to an electric telescopic rod or a hydraulic telescopic rod, the movable end of the second lifting device 10 can penetrate through the limiting ring 3, the bottom of the culture dish 4 is connected with the first magnetic plate 30, the movable end of the second lifting device 10 is provided with a second magnetic plate 11, the second magnetic plate 11 can be mutually adsorbed with the first magnetic plate 30, meanwhile, the matching of the two magnetic plates plays a role in positioning the culture dish, so that the annular groove at the bottom of the culture dish 4 can be better in inserting and matching with the limiting rings, and the second magnetic plate 5 can be positioned on the inner side of each limiting block 6 composition area, stopper 6 can play the limiting action to the horizontal migration of first magnetic sheet 30, the position of culture dish can not change when making culture dish and elastic seal contact, the magnetic adsorption of first magnetic sheet and second magnetic sheet simultaneously makes second elevating gear 10 can take out the culture dish from the elastic seal downwards, and because adopt the magnetic adsorption cooperation between culture dish 4 and the second elevating gear 10, after second elevating gear 10 places culture dish 4 on the spacing ring, because the spacing ring is fixed with the culture dish, second elevating gear only need the straight continuation downwards can realize with this culture dish between 4 separation, be convenient for second elevating gear 10 carries out ascending jacking respectively to each culture dish.
The bottom installation of incubator 1 holds case 15, holds case 15 and holds second elevating gear 10 wherein, and the movable rod of second elevating gear 10 upwards passes incubator 1 and for the sealed contact cooperation between its movable rod outside and the incubator 1 to guarantee the good sealed environment in the incubator 1.
The inner side of the first vertical pipe 2 is provided with a second vertical pipe 18 which is fixedly arranged in a contact and matching manner, the outer side of the first vertical pipe 2 is uniformly provided with a plurality of second through holes 19, the outer side of the second vertical pipe 18 is provided with third through holes 20 corresponding to the second through holes 19 one by one, the outer ends of the second through holes 19 can be provided with water mist nozzles 21, the alignment and the staggering of the second through holes 19 and the third through holes 20 are controlled by the rotation of the first vertical pipe 2, the upper end of the first vertical pipe 2 is connected with a driving device 22, the driving device 22 comprises but is not limited to a motor or a motor, the driving device 22 can drive the first vertical pipe 2 to rotate, the upper end of the second vertical pipe 18 upwards passes through the first vertical pipe 2 and then is communicated with a cold air supply device, the upper end of the second vertical pipe 18 is provided with a first one-way electromagnetic valve 23, the cold air supply device can supply cold air into the second vertical pipe 18 when the low-temperature environment is needed in the incubator, the bottom of the incubator 1 is provided with a water tank 14, the bottom of the water tank 14 is flush with the containing box 15, a heating module 16 and a water pump 17 are installed in the water tank 14, the heating module 16 can heat water in the water tank, the lower end of the second vertical pipe 18 extends into the water tank 14 and then is connected with the water pump 17, a plurality of air inlet holes 24 are formed in the lower portion of the second vertical pipe 18, when high-temperature disinfection is needed or the temperature needs to be increased in the incubator, steam generated after the water in the water tank is heated by the heating module 16 can enter the second vertical pipe 18 through the air inlet holes 24, the water pump 17 can supply water to the incubator 1 through the second vertical pipe 18, a second one-way electromagnetic valve 25 is installed at the lower end of the second vertical pipe 18, the second one-way electromagnetic valve 25 is located on the upper side of the air inlet holes 14, a plurality of blades 26 are connected to the outer side of the lower portion of the first vertical pipe 2, the blades generate wind power when rotating along with the first vertical pipe, when the incubator 1 is completely sealed by the door structure, the wind power generated by the rotation of the blades can help cold air or the steam to quickly diffuse in the incubator, when the door structure is opened, the blade can rapidly exhaust the air in the incubator 1, one side of the top of the incubator 1 can be provided with a pressure release valve 27, and the pressure can be automatically released when the pressure in the incubator is too high.
The working process of the device is as follows: when stem cells are not placed in the incubator for culture, water in the water tank can be boiled and water vapor is generated through heating work of the heating module, the water vapor enters the second vertical pipe 18 through the air inlet hole, the door structure is in a closed state at the moment, the driving device can be controlled to drive the first vertical pipe to rotate, when the second through hole and the third through hole are overlapped, the vapor enters the incubator, and meanwhile, the blades rotate to generate wind power so that the water vapor rapidly permeates the incubator to achieve the effect of high-temperature disinfection; when the culture medium in the culture dish needs to be stored at low temperature, the cold air supply device is controlled to supply cold air to the culture box, so that the temperature in the culture box is kept low, and the requirement of the culture medium is met; when cells are placed in the culture dish for culture, the temperature in the culture box can be correspondingly regulated through the supply of hot air and cold air, meanwhile, when the hot air or the cold air is input into the culture box, the first vertical pipe drives each culture dish and the cells in the culture dish to move, so that each culture dish can be uniformly heated or cooled, the single variable of the rectal adenocarcinoma cells required by the temperature and the humidity in the culture process is ensured, meanwhile, the blades rotate to enable the entering cold air or the entering hot air to rise upwards, the cold air or the entering hot air cannot directly act on the cells in the culture dish, and the possibility of cell damage is reduced; when the humidity in the incubator needs to be improved, the water pump can work to spray the water in the water tank into the incubator from the atomizing nozzle, and meanwhile, the water mist can quickly permeate the incubator under the action of wind power generated by the rotation of the culture dishes driven by the first vertical pipe and the rotation of the blades, so that the humidity of each culture dish is uniformly improved; when a user needs to observe the rectal adenocarcinoma cells in the culture dish, the first vertical pipe is driven by the driving device to rotate by a certain angle, so that the culture dish is aligned to the second magnetic plate on the upper side of the second lifting device, then the movable end of the second lifting device is controlled to drive the second magnetic plate to move upwards, the second magnetic plate is contacted with the first magnetic plate and jacks up the culture dish, the limiting ring is separated from the annular groove on the culture dish until the outer wall of the culture dish is contacted and matched with the inner wall of the elastic sealing ring, the culture dish seals the culture box at the moment, the door structure is opened, and the second lifting device drives the culture dish to continuously move upwards to a height suitable for the observation of a microscope, so that the user can clearly observe the rectal adenocarcinoma cells; after the observation finishes, the cooperation of the first magnetic plate of second elevating gear accessible and second magnetic plate drives the lower extreme of culture dish downstream to first through-hole, keep the closure to the incubator, close the door structure simultaneously, then second elevating gear drives the culture dish and returns to the normal position, the spacing ring is kept away from downwards to the expansion end of second elevating gear, make the spacing ring can switch other culture dishes to the downside of first through-hole under the drive of first standpipe, operate according to this when needs observe the rectum adenocarcinoma cell in other culture dishes can.
The above-described embodiments of the present invention do not limit the scope of the present invention. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.
Claims (10)
1. A stem cell biological growth observation device for rectal adenocarcinoma treatment is characterized in that: including the incubator, be located the cell observation device of incubator upside, rotate the supply tube device of setting at the incubator middle part, the supply tube device can supply with steam to the incubator, air conditioning, the water smoke, be equipped with a plurality of culture dishes in the incubator, the culture dish encircles the outside evenly distributed at the supply tube device, the culture dish all links to each other with the supply tube device, the supply tube device links to each other with drive arrangement, drive arrangement can drive the rotation tube part of supply tube device and drive the culture dish and rotate, set up first through-hole on the incubator and supply the culture dish to come in and go out, the outer end of first through-hole is equipped with the door structure that can carry out the switching to first through-hole, cell observation device can observe the cell that is located the culture dish in first through-hole.
2. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 1, wherein: the cell observation device is a microscope.
3. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 1, wherein: the utility model discloses a culture dish, including the first standpipe of rotation setting and the second standpipe that is located first standpipe internal fixation setting, the outer wall of second standpipe and the inner wall contact cooperation of first standpipe, first standpipe passes through the connecting rod and links to each other with the culture dish, first standpipe links to each other with drive arrangement, drive arrangement can drive first standpipe rotation, a plurality of second through-holes have been seted up in the outside of first standpipe, the third through-hole has been seted up one by one to the outside of second standpipe corresponding second through-hole, the alignment and the staggering of second through-hole and third through-hole can be controlled in the rotation of first standpipe, the one end of first standpipe links to each other with air conditioning feeding mechanism, the other end of first standpipe links to each other with water smoke steam feeding mechanism, the outside of second standpipe is connected with the blade.
4. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 3, characterized in that: and the outer ends of the second through holes are provided with water mist nozzles.
5. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 3, characterized in that: the water mist hot gas supply device comprises a water tank, the water tank is installed at the bottom of the incubator, the bottom of the water tank is flush with the accommodating box, a heating module and a water pump are installed in the water tank, the lower end of a second vertical pipe is connected with the water pump, a plurality of air inlet holes are formed in the lower portion of the second vertical pipe, a second one-way electromagnetic valve is arranged at the lower end of the second vertical pipe and located at the upper side of the air inlet holes, a first one-way electromagnetic valve is arranged on the upper portion of a first vertical pipe, and the upper end of the first vertical pipe is connected with the air conditioning supply device.
6. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 5, wherein: and a pressure release valve is arranged on the incubator.
7. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 3, characterized in that: the outer side of the first vertical pipe is annularly and uniformly connected with a plurality of connecting rods, the outer ends of the connecting rods are connected with limiting rings, culture dishes are arranged on the upper sides of the limiting rings, annular grooves are formed in the bottoms of the culture dishes corresponding to the limiting rings, the limiting rings can be inserted into the annular grooves, the culture dishes are annularly and uniformly distributed on the outer sides of the first vertical pipes, elastic sealing rings are arranged in the first through holes, the culture dishes can be in sealing contact with the elastic sealing rings, door structures capable of being opened and closed are arranged at the outer ends of the first through holes, the door structures can completely seal the first through holes, a plurality of limiting blocks are annularly and uniformly distributed at the bottoms of the culture dishes around the centers of the bottom of the culture dishes, the limiting blocks are positioned on the outer sides of the first magnetic plates, second lifting devices are arranged on the lower sides of the limiting rings, the movable ends of the second lifting devices can penetrate through the limiting rings, the bottoms of the culture dishes are connected with first magnetic plates, and second magnetic plates are mounted at the movable ends of the second lifting devices, the second magnetic plate can be attracted to the first magnetic plate.
8. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 7, wherein: the second lifting device is an electric telescopic rod, and a movable rod of the electric telescopic rod upwards penetrates through the side wall of the incubator and is in contact fit with the side wall.
9. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 8, wherein: the bottom installation of incubator holds the case, holds the incasement and is equipped with electric telescopic handle's fixed part.
10. The apparatus for observing the biological growth of stem cells for the treatment of rectal adenocarcinoma according to claim 1, wherein: the cell observation device is connected with the incubator through a first lifting device.
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| CN202210081222.5A CN114395479A (en) | 2022-01-24 | 2022-01-24 | Stem cell biological growth observation device for rectal adenocarcinoma treatment |
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| CN202210081222.5A CN114395479A (en) | 2022-01-24 | 2022-01-24 | Stem cell biological growth observation device for rectal adenocarcinoma treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116162547A (en) * | 2023-03-01 | 2023-05-26 | 诸城舜沃农业科技有限公司 | Strain fermentation incubator and use method |
| CN116355756A (en) * | 2023-03-11 | 2023-06-30 | 深圳医爱健康管理有限公司 | Constant temperature incubator of cell culture |
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| CN116355756A (en) * | 2023-03-11 | 2023-06-30 | 深圳医爱健康管理有限公司 | Constant temperature incubator of cell culture |
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