CN116138219A - Device and method for breeding black oocysts of noctuid - Google Patents
Device and method for breeding black oocysts of noctuid Download PDFInfo
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- CN116138219A CN116138219A CN202310108703.5A CN202310108703A CN116138219A CN 116138219 A CN116138219 A CN 116138219A CN 202310108703 A CN202310108703 A CN 202310108703A CN 116138219 A CN116138219 A CN 116138219A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention provides a breeding device and a breeding method for black bees of noctuid, belonging to the technical field of agriculture and forestry biology. The breeding device comprises a box body, nylon cloth is coated on two sides and the rear portion of the box body, a box door is arranged on the front portion of the box body, a white light lamp is arranged on the inner side of the top of the box body, a partition plate is arranged on the top and the bottom of the box body, a humidity control device is arranged on the partition plate, a nylon wire is fixed on the box body, and the distance between the nylon wire and the top of the box body is 3-5 cm. The breeding device is suitable for large-scale breeding, and has the advantages of simple structure, low manufacturing cost, convenient use, large parasitic space and large breeding quantity. Meanwhile, the breeding device is utilized to breed the noctuid black egg bees, so that the breeding success rate, the spawning parasitic rate and the productivity of the noctuid black egg bees are improved, the adult bee life is prolonged, and the method has important significance in large-scale breeding application of the noctuid black egg bees.
Description
Technical Field
The invention belongs to the technical field of agriculture and forestry biology, and particularly relates to a black egg bee breeding device and a breeding method thereof.
Background
The black egg bees of the noctuid belong to the Platygastrostrip of Hymenoptera wide-belly fine bees of Hymenoptera, and are main parasitic natural enemies of the noctuid in the ovum period. As early as the 70 s of the 20 th century, related researches on spodoptera exigua have been reported abroad, and so far, many lamei countries have achieved some successful experience in preventing spodoptera exigua by using spodoptera exigua or other spodoptera exigua host eggs to reproduce spodoptera exigua. Brazil also has the scholars successfully breeds the black wasps of the noctuid by using the eggs of the noctuid, however, both domestic Huo Liangxiao and Dai Peng recent researches indicate that the current domestic attempts of breeds the black wasps of the noctuid by using the eggs of the noctuid are unsuccessful. The method is characterized in that a domestic scholars breeds the noctuid black egg bees by utilizing the noctuid eggs, and morphological characteristics of each insect state in the development process of the noctuid black egg bees are systematically observed; huo Liangxiao and the like, by breeding the Spodoptera litura with the Spodoptera litura, the biological characteristics of the Spodoptera litura parasitic on the Spodoptera litura were observed. However, at present, whether the noctuid black egg bees are bred in a small scale or in an indoor laboratory, a large-scale breeding technology system of the noctuid black egg bees is established, which has important significance for long-term prevention and control of noctuid pests, and based on the deficiency of the prior art, great demands are currently placed on a method capable of breeding the noctuid black egg bees in a large scale, so that a device and a method for breeding noctuid black egg bees are urgently needed to be explored, and the large-scale production of the noctuid black egg bees is realized.
Disclosure of Invention
In view of the above, the invention aims to provide a device and a method for breeding noctuid black egg bees, which improve the breeding success rate, spawning parasitic rate and productivity of noctuid black egg bees, prolong the adult bee life, and have low breeding cost, thereby being beneficial to large-scale breeding of noctuid black egg bees and realizing artificial rapid large-scale breeding and long-term subculture of noctuid black egg bees.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a black egg bee breeding device for noctuid, which comprises a box body, wherein nylon cloth is coated on two sides and the rear part of the box body, a box door is arranged at the front part of the box body, white light lamps are arranged on the inner side of the top of the box body, partition plates are arranged on the top and the bottom of the box body, a humidity control device is arranged on the partition plates, nylon wires are fixed on the box body, and the distance between the nylon wires and the top of the box body is 3-5 cm.
Preferably, the breeding device further comprises a carrier medium for the host eggs and a bee breeding device.
Preferably, the box body is provided with a temperature and humidity detection device.
Preferably, the box door is a glass door; the specification of the white light lamp is T5-12W/0.9 m; the humidity control device is a wet foam box, and the moisture content of the wet foam is 10% -15%.
Preferably, the bearing medium comprises a non-woven fabric, the non-woven fabric is white in specification, 30g/m, 120cm in length, 40cm in width and 0.05mm in thickness; the bee breeding device is a three-dimensional plastic box, moist roll paper with 10% -12% of moist degree is placed at the bottom of the three-dimensional plastic box, 6-8 ventilation small holes are formed in the top of the three-dimensional plastic box, and the aperture of each ventilation small hole is 0.8-1.2 mm.
The invention provides a method for breeding black wasps of noctuid by using the breeding device, which comprises the following steps:
suspending a bearing medium containing 8% -10% of honey water on a nylon wire, putting the spodoptera exigua adult wasp and spodoptera exigua egg cloth in a breeding device, suspending the spodoptera exigua egg cloth on the nylon wire, carrying out spawning parasitic of the spodoptera exigua adult wasp, taking out the spodoptera exigua egg cloth which has been parasitized by the spodoptera exigua adult wasp after 24-48 h, then putting the spodoptera exigua egg cloth in a breeding device, and finally carrying out adult wasp breeding in the breeding device.
Preferably, the ratio of the adult bees of the black-egg bees of the noctuid to the eggs of the spodoptera frugiperda is 1:30-50.
Preferably, the breeding conditions of the spawning parasitic are as follows: the illumination L is that D=14-16:10-8, the temperature is 26-28 ℃, and the air humidity is 60-70%.
Preferably, the conditions for breeding the adult bees are as follows: the temperature is 26-28 ℃, the illumination L is D=14-16:10-8, the air humidity is 60-70%, and the breeding time is 9-11D.
Preferably, the spodoptera frugiperda eggs are spodoptera frugiperda eggs within 24-48 hours of the adult spodoptera frugiperda production.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a breeding device and a breeding method for black wasps of noctuid, and the breeding device is suitable for large-scale breeding, and has the advantages of simple structure, low manufacturing cost, convenient use, large parasitic space and large breeding quantity. Meanwhile, the breeding device is utilized to breed the noctuid black egg bees, so that the breeding success rate, the spawning parasitic rate and the productivity of the noctuid black egg bees are improved, the adult bee life is prolonged, and the method has important significance in large-scale breeding application of the noctuid black egg bees.
Drawings
Fig. 1 is a schematic diagram of the overall structure of a black egg bee breeding device for noctuid;
the device comprises a box body-1, a non-woven fabric-2, a box door-3, a white light lamp-4, a partition plate-5, a humidity control device-6, a nylon wire-7, a carrier medium-8 of parasitic eggs, a bee breeding device-9, a temperature and humidity detection device-10, a foot rest-11 and a clip-12.
Detailed Description
The invention provides a black egg bee breeding device for noctuid, which comprises a box body, wherein nylon cloth is coated on two sides and the rear part of the box body, a box door is arranged at the front part of the box body, white light lamps are arranged on the inner side of the top of the box body, partition plates are arranged on the top and the bottom of the box body, a humidity control device is arranged on the partition plates, nylon wires are fixed on the box body, and the distance between the nylon wires and the top of the box body is 3-5 cm.
In the invention, the black egg bee breeding device for the noctuid can comprise one layer of box body or more than two layers of box bodies, wherein the length of the box bodies is 115-125 cm, the width of the box bodies is 35-45 cm, and the height of the box bodies is 30-37 cm. Taking an example that the noctuid black egg bee breeding device comprises five layers of boxes, the length of the noctuid black egg bee breeding device is 115-125 cm, the width of the noctuid black egg bee breeding device is 35-45 cm, and the height of the noctuid black egg bee breeding device is 195-205 cm; the length of the box body is 115-125 cm, the width is 35-45 cm, and the height is 30-37 cm. The breeding device is further provided with a foot rest, the height of the foot rest is 10-55 cm, the foot rest is made of stainless steel, and the foot rest is a stainless steel column. The breeding device also preferably comprises a carrier medium of the host eggs and a bee breeding device. The breeding device expands the breeding space range, increases the breeding quantity of the noctuid black egg bees, and meets the requirement of large-scale noctuid black egg bees breeding.
In the invention, the box door is a glass door, and further preferably two glass doors sliding left and right.
In the invention, three sides of two sides and the rear part of the box body are white nylon cloth, the specification of the nylon cloth at the rear side is length multiplied by width=120 cm multiplied by 35cm, the specification of the nylon cloth at the left side and the right side is length multiplied by width=40 cm multiplied by 35cm, the nylon cloth is 110-130 meshes, and the nylon cloth is purchased from Shanghai Xingan screen manufacturing plant. According to the invention, the top of the box body is changed into the partition plate, the two sides and the three sides of the rear part of the box body are made of nylon cloth, gas exchange is carried out through the nylon cloth with 120 meshes, perforation and ventilation are not needed, the design and manufacturing cost is low, and the large-scale breeding is easy.
In the invention, in view of the fact that links such as illumination, temperature, humidity and the like in the egg laying and breeding of the black bees of the noctuid are particularly important, the temperature in the breeding box body is kept through the room air conditioner, and a heating or cooling device is not needed to be arranged on the breeding device, so that the breeding device is simpler and more convenient, the temperature is regulated and controlled conveniently, and the cost is lower. The box body is provided with the temperature and humidity detection device, the temperature probe of the temperature and humidity detection device is positioned in the breeding box body, the temperature display is positioned on the right side outside the box body, and further preferably, the box body is provided with 1 temperature and humidity detection device, and the detection device on each layer of breeding box body is connected with the display, so that the temperature and humidity conditions in the breeding box body can be observed manually conveniently, the optimal parasitic spawning environment of the black bees of the noctuid is ensured, and the parasitic success rate of the black bees of the noctuid is improved. The white light lamp is arranged on the inner side of the top of the box body, and further preferably, 1 white light lamp is arranged on the inner side of the top of the box body, and the specification of the white light lamp is preferably T5-12W/0.9 m. The humidity control device is a wet foam box, the moisture content of the wet foam is 10% -15%, the wet foam box is used for adjusting the air humidity required by breeding and mating and spawning of the black wasps of the noctuid, water is sprayed into the box every day by using a watering can, the moisture content of the wet foam in the box is kept to be 10% -15%, and the breeding air humidity is 60% -70%, so that the moisturizing effect of a breeding space of the adult wasps is achieved. The plastic round box with the specification diameter of 9-11 cm and the height of 4.6-5.0 cm is more preferable to be a plastic round box with the diameter of 10cm and the height of 4.8 cm.
In the present invention, the carrier medium preferably comprises a nonwoven fabric, the nonwoven fabric has a length of 120cm, a width of 40cm and a thickness of 0.05mm, and the nonwoven fabric is a white nonwoven fabric. The bee breeding device is preferably a three-dimensional plastic box, moist roll paper with the moist degree of 10% -12% is placed at the bottom of the three-dimensional plastic box, 6-8 ventilation small holes are formed in the top of the three-dimensional plastic box, and the aperture of each ventilation small hole is preferably 0.8-1.2 mm. The three-dimensional plastic box is preferably in the form of length×width×height=21 cm×15cm×7cm, and the roll paper is preferably in the form of 2 to 3 layers (each 2 layers having a thickness of 0.1 mm). When adult bees are bred, the invention comprehensively considers that the wet cotton cloth has low water absorption and fast evaporation, the cotton cloth is required to be cut, and the use is inconvenient, so the roll paper which is consistent with the size of the three-dimensional box can be selected, the roll paper is convenient to use, is economical and practical, has the characteristics of high water absorption, can keep moisture, keeps the humidity in the box, has smaller hardness than the cotton cloth, is easy to fold and has high plasticity.
The invention provides a method for breeding black wasps of noctuid by using the breeding device, which comprises the following steps:
suspending a bearing medium containing 8% -10% of honey water on a nylon wire, putting the spodoptera exigua adult wasp and spodoptera exigua egg cloth in a breeding device, suspending the spodoptera exigua egg cloth on the nylon wire, carrying out spawning parasitic of the spodoptera exigua adult wasp, taking out the spodoptera exigua egg cloth which has been parasitized by the spodoptera exigua adult wasp after 24-48 h, then putting the spodoptera exigua egg cloth in a breeding device, and finally carrying out adult wasp breeding in the breeding device.
In the invention, a bearing medium containing 8-10% of honey water is hung on a nylon wire. The invention can fix the bearing medium by the clip to hang on the nylon wire. The bearing medium is preferably non-woven fabric, and 8-10% of the non-woven fabric is soaked by 8-10% of the honey water to obtain the non-woven fabric containing 8-10% of the honey water. The spodoptera frugiperda ovum is used as a main material for parasitic breeding of the spodoptera frugiperda ovum, the embryo development of a host possibly depletes nutrient substances in the ovum, and the spodoptera frugiperda ovum can not obtain nutrition from the host embryo in the later development stage, so that 8:30-10:00 am of the spodoptera frugiperda ovum is added, the non-woven fabric containing 8-10% of honey water can provide the required nutrition for the spodoptera frugiperda ovum, the service life of 1/3 adult bees can be prolonged, the parasitic ovum quantity and the filial generation eclosion rate of the spodoptera frugiperda ovum in the breeding process can be improved. The honey type required by breeding the noctuid black egg bees is not particularly limited, and the honey sold in the market in the field can be adopted.
In the invention, after a bearing medium containing 8% -10% of honey water is hung on a nylon wire, adult noctuid black wasp and spodoptera frugiperda egg cloth are put into a breeding device, and simultaneously, the spodoptera frugiperda egg cloth is hung on the nylon wire to carry out spawning parasitic of the adult noctuid black wasp. The spodoptera frugiperda egg cloth can be fixed by the clip so as to be hung on the nylon wire. The ratio of the adult black-egg bees to the spodoptera frugiperda eggs is preferably 1:30-50, more preferably 1:40, for example, 9000-11000 adult black-egg bees of the spodoptera frugiperda in each breeding box body and 360000 ～ 440000. The breeding conditions of the spawning parasitism are preferably as follows: the illumination L is that D=14-16:10-8, the temperature is 26-28 ℃, and the air humidity is 60-70%.
In the invention, 24-48 hours later, spodoptera frugiperda egg cloth parasitized by spodoptera frugiperda black egg bees is taken out and then put into a bee breeding device, and finally adult bee breeding is carried out in the bee breeding device. As an implementation mode, the residue of the black egg bees in the breeding box body is removed every day, the spodoptera frugiperda egg cloths which are already in the black egg bees breeding box body for 24-48 hours are taken out, and new spodoptera frugiperda egg cloths are replaced, so that the box body is kept clean. In the process of breeding the noctuid black egg bees, in order to ensure that the adult noctuid black egg bees with high quality are bred, the conditions for breeding the adult noctuid black egg bees are preferably as follows: the temperature is 26-28 ℃, the illumination L is D=14-16:10-8, the air humidity is 60-70%, and the breeding time is 9-11D. The spodoptera frugiperda eggs are preferably spodoptera frugiperda eggs within 24-48 hours of the adult spodoptera frugiperda production. Before breeding adult bees, the roll paper with the thickness of 2-3 layers (each 2 layers have the thickness of 0.1 mm) is horizontally placed at the bottom of a plastic box, and the roll paper is sprayed by a spray can to wet, so that the method for wetting the roll paper is not particularly limited, and only the wettability of the roll paper in the box is kept at 10% -12%. After adult bees are bred, water is sprayed into the box every day, the water content is kept at 10% -12%, humidity is provided for the adult bees breeding space, and the requirement of the growth environment of the black bees of the noctuid is met.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A method for breeding adult noctuid black egg bees by utilizing a noctuid black egg bees breeding frame comprises the following steps:
the method comprises the steps of placing emerging black-egg bees of the newly emerged noctuid on a breeding frame with the specification of length, width and height of 120cm, 40cm and 200cm for spawning and parasitizing of bees, wherein the breeding frame is 5 layers in total, the specification of each layer of breeding box body is length, width and height of 120cm, 40cm and 35cm, the height of frame feet is 25cm, two glass doors capable of sliding left and right are arranged at the front part of the box body, 120-mesh nylon cloths are arranged at the two sides and the rear part of the box body, and partition boards are arranged at the top and the bottom of the box body. And 1 led white light lamp with the specification of T5-12W/0.9 m is arranged at the position 4cm away from the top of each layer of breeding box body to provide illumination required by the whole growth period of the noctuid black egg bees, the illumination time L of environmental conditions is D=16:8, the temperature in the breeding box body is regulated through a room air conditioner, and the whole environmental temperature is kept at 27 ℃. The moisture content of the wet foam in the plastic round box with the specification diameter of 10cm and the height of 4.8cm is 10% -15%, the wet foam is used for adjusting the air humidity required by breeding mating and spawning of the adult noctuid black bees, water is sprayed into the wet foam box by using a watering can every day, the moisture content of the wet foam in the box is kept to be 10% -15%, the breeding air humidity is 60% -70%, humidity is provided for the breeding space of the noctuid black bees, and the requirement on the growth environment of the noctuid black bees is met. Four nylon wires are fixed at the position of the breeding box, which is 4cm away from the top, the nylon wires are provided with clamps for fixing spodoptera littoralis egg cloths parasitic to spodoptera littoralis adult bees to lay eggs in egg blocks on the spodoptera littoralis egg cloths, each layer of box body on the breeding frame is provided with 1 temperature and humidity detection device, each layer of temperature and humidity detection device on the breeding box body is connected with a display, and the conditions of temperature and humidity in each layer of breeding box body are manually required to be observed, and the activity condition of the spodoptera littoralis bees is observed. Soaking the non-woven fabric with 8-10% of honey water, fixing the non-woven fabric containing 8-10% of honey water on a nylon wire in a breeding box body by using a clamp, and adding the non-woven fabric containing 8-10% of honey water into the box body at 8:30-10:00 am during breeding so as to supplement nutrition for adult black bees of the noctuid.
(2) Placing egg cloths within 24-48 hours under the spodoptera frugiperda in a breeding box for breeding spodoptera frugiperda black egg bees, then hanging the egg cloths on nylon wires in the box, and fixing the egg cloths by using a clamp. Placing adult noctuid black wasp into a breeding frame under the environmental conditions of 27 ℃ and 60% -70% of breeding air humidity and D=16:8 of illumination L to carry out spawning parasitism of the adult noctuid black wasp, taking the spodoptera frugiperda eggs as hosts for the parasitic breeding of the noctuid black wasp, wherein the density of adult noctuid black wasp in each layer of breeding box body is 9000-11000 head wasps, and the parasitizable egg size is 360000 ～ 440000.
(3) And (3) removing cadaver residues after the night moth black wasps are parasitized, taking out the meadow greedy egg cloth parasitized by the night moth black wasps after 24 hours, and keeping the interior of the breeding box clean.
(4) A three-dimensional plastic box with the specification of length multiplied by width multiplied by height=21 cm multiplied by 15cm multiplied by 7cm is selected, 6-8 ventilation holes with the aperture of 1mm are punched on a box cover, roll paper with the thickness of 2-3 layers (the thickness of each 2 layers is 0.1 mm) is horizontally placed at the bottom of the plastic box, the roll paper is wetted by using a watering can, the roll paper wettability in the plastic box is kept at 10% -12%, spodoptera littoralis eggs which are parasitized by spodoptera littoralis are placed in the plastic box with the wetted roll paper, a date cover is covered, the plastic box containing spodoptera littoralis eggs which are parasitized by spodoptera littoralis is placed in a breeding frame under the environmental conditions that the temperature is 27 ℃, the breeding air humidity is 60% -70%, and the illumination L is D=16:8, and adult bee breeding is carried out for 10D.
Example 2
A method for breeding adult noctuid black egg bees by utilizing a noctuid black egg bees breeding frame comprises the following steps:
the method comprises the steps of placing emerging black-egg bees of the newly emerged noctuid on a breeding frame with the specification of length, width and height of 120cm, 40cm and 200cm for spawning and parasitizing of bees, wherein the breeding frame is 5 layers in total, the specification of each layer of breeding box body is length, width and height of 120cm, 40cm and 35cm, the height of frame feet is 25cm, two glass doors capable of sliding left and right are arranged at the front part of the box body, 120-mesh nylon cloths are arranged at the two sides and the rear part of the box body, and partition boards are arranged at the top and the bottom of the box body. Each layer of breeding box body is provided with 1 led white light lamp with the specification of T5-12W/0.9 m at the position 4cm away from the top to provide illumination required by the whole growth period of the noctuid black egg bees, and the illumination time L of environmental conditions is as follows: d=15: 9, adjusting the temperature in the breeding box body by room air conditioning and keeping the whole environment temperature at 26 ℃. The moisture content of the wet foam in the plastic round box with the specification diameter of 10cm and the height of 4.8cm is 10% -15%, the wet foam is used for adjusting the air humidity required by breeding mating and spawning of the adult noctuid black bees, water is sprayed into the wet foam box by using a watering can every day, the moisture content of the wet foam in the box is kept to be 10% -15%, the breeding air humidity is 60% -70%, humidity is provided for the breeding space of the noctuid black bees, and the requirement on the growth environment of the noctuid black bees is met. Four nylon wires are fixed at the position of the breeding box, which is 4cm away from the top, the nylon wires are provided with clamps for fixing spodoptera littoralis egg cloths parasitic to spodoptera littoralis adult bees to lay eggs in egg blocks on the spodoptera littoralis egg cloths, each layer of box body on the breeding frame is provided with 1 temperature and humidity detection device, each layer of temperature and humidity detection device on the breeding box body is connected with a display, and the conditions of temperature and humidity in each layer of breeding box body are manually required to be observed, and the activity condition of the spodoptera littoralis bees is observed. Soaking the non-woven fabric with 8-10% of honey water, fixing the non-woven fabric containing 8-10% of honey water on a nylon wire in a breeding box body by using a clamp, and adding the non-woven fabric containing 8-10% of honey water into the box body at 8:30-10:00 am during breeding so as to supplement nutrition for adult black bees of the noctuid.
(2) Placing egg cloths within 24-48 hours under the spodoptera frugiperda in a breeding box for breeding spodoptera frugiperda black egg bees, then hanging the egg cloths on nylon wires in the box, and fixing the egg cloths by using a clamp. Placing adult noctuid black wasp into a breeding frame under the environmental conditions of temperature 26 ℃ and breeding air humidity 60% -70% and light L: D=15:9, carrying out spawning parasitism on the adult noctuid black wasp, using the spodoptera frugiperda eggs as hosts for the parasitic breeding of the noctuid black wasp, wherein the density of adult noctuid black wasp in each layer of breeding box body is 9000-11000 head wasps, and the parasitizable egg size is 360000 ～ 440000.
(3) And (3) removing cadaver residues after the night moth black wasps are parasitized, taking out the meadow greedy egg cloth parasitized by the night moth black wasps after 24 hours, and keeping the interior of the breeding box clean.
(4) A three-dimensional plastic box with the specification of length multiplied by width multiplied by height=21 cm multiplied by 15cm multiplied by 7cm is selected, 6-8 ventilation holes with the aperture of 1mm are punched on a box cover, roll paper with the thickness of 2-3 layers (the thickness of each 2 layers is 0.1 mm) is horizontally placed at the bottom of the plastic box, the roll paper is wetted by using a watering can, the roll paper wettability in the plastic box is kept at 10% -12%, spodoptera littoralis eggs which are parasitized by spodoptera littoralis are placed in the plastic box with the wetted roll paper, a date cover is covered, the plastic box containing spodoptera littoralis eggs which are parasitized by spodoptera littoralis is placed in a breeding frame under the environmental conditions that the temperature is 27 ℃, the breeding air humidity is 60% -70%, and the illumination L is D=16:8, and adult bee breeding is carried out for 11D.
Example 3
A method for breeding adult noctuid black egg bees by utilizing a noctuid black egg bees breeding frame comprises the following steps:
the method comprises the steps of placing emerging black-egg bees of the newly emerged noctuid on a breeding frame with the specification of length, width and height of 120cm, 40cm and 200cm for spawning and parasitizing of bees, wherein the breeding frame is 5 layers in total, the specification of each layer of breeding box body is length, width and height of 120cm, 40cm and 35cm, the height of frame feet is 25cm, two glass doors capable of sliding left and right are arranged at the front part of the box body, 120-mesh nylon cloths are arranged at the two sides and the rear part of the box body, and partition boards are arranged at the top and the bottom of the box body. Each layer of breeding box body is provided with 1 led white light lamp with the specification of T5-12W/0.9 m at the position 4cm away from the top to provide illumination required by the whole growth period of the noctuid black egg bees, and the illumination time L of environmental conditions is as follows: d=14: 10, the temperature in the breeding box body is regulated by room air conditioning and the whole environment temperature is kept at 28 ℃. The moisture content of the wet foam in the plastic round box with the specification diameter of 10cm and the height of 4.8cm is 10% -15%, the wet foam is used for adjusting the air humidity required by breeding mating and spawning of the adult noctuid black bees, water is sprayed into the wet foam box by using a watering can every day, the moisture content of the wet foam in the box is kept to be 10% -15%, the breeding air humidity is 60% -70%, humidity is provided for the breeding space of the noctuid black bees, and the requirement on the growth environment of the noctuid black bees is met. Four nylon wires are fixed at the position of the breeding box, which is 4cm away from the top, the nylon wires are provided with clamps for fixing spodoptera littoralis egg cloths parasitic to spodoptera littoralis adult bees to lay eggs in egg blocks on the spodoptera littoralis egg cloths, each layer of box body on the breeding frame is provided with 1 temperature and humidity detection device, each layer of temperature and humidity detection device on the breeding box body is connected with a display, and the conditions of temperature and humidity in each layer of breeding box body are manually required to be observed, and the activity condition of the spodoptera littoralis bees is observed. Soaking the non-woven fabric with 8-10% of honey water, fixing the non-woven fabric containing 8-10% of honey water on a nylon wire in a breeding box body by using a clamp, and adding the non-woven fabric containing 8-10% of honey water into the box body at 8:30-10:00 am during breeding so as to supplement nutrition for adult black bees of the noctuid.
(2) Placing egg cloths within 24-48 hours under the spodoptera frugiperda in a breeding box for breeding spodoptera frugiperda black egg bees, then hanging the egg cloths on nylon wires in the box, and fixing the egg cloths by using a clamp. Placing adult noctuid black wasp into a breeding frame under the environmental conditions of 28 ℃ and 60% -70% of breeding air humidity and D=14:10 of illumination L to carry out spawning parasitism of the adult noctuid black wasp, taking the spodoptera frugiperda eggs as hosts for the parasitic breeding of the noctuid black wasp, wherein the density of adult noctuid black wasp in each layer of breeding box body is 9000-11000 head wasps, and the parasitizable egg size is 360000 ～ 440000.
(3) And (3) removing cadaver residues after the night moth black wasps are parasitized, taking out the meadow greedy egg cloth parasitized by the night moth black wasps after 24 hours, and keeping the interior of the breeding box clean.
(4) A three-dimensional plastic box with the specification of length multiplied by width multiplied by height=21 cm multiplied by 15cm multiplied by 7cm is selected, 6-8 ventilation holes with the aperture of 1mm are punched on a box cover, roll paper with the thickness of 2-3 layers (the thickness of each 2 layers is 0.1 mm) is horizontally placed at the bottom of the plastic box, the roll paper is wetted by using a watering can, the roll paper wettability in the plastic box is kept at 10% -12%, spodoptera littoralis eggs which are parasitized by spodoptera littoralis are placed in the plastic box with the wetted roll paper, a date cover is covered, the plastic box containing spodoptera littoralis eggs which are parasitized by spodoptera littoralis is placed in a breeding frame under the environmental conditions that the temperature is 27 ℃, the breeding air humidity is 60% -70%, and the illumination L is D=16:8, and adult bee breeding is carried out for 9D.
Example 4
The utility model provides a black egg bee adult bee breeds frame, breeds the frame specification and is length x width x height = 120cm x 40cm x 200cm breeds the frame and carries out the spawning parasitism of bee, breeds the frame and breeds 5 layers in total, and every layer breeds the box specification and is length x width x height = 120cm x 40cm x 35cm, and the frame foot height is 25cm, and the frame foot is the stainless steel column, and 1 piece specification is T5-12W/0.9 meter's led white light lamp is equipped with at every layer breeds the box top, the box front portion is provided with two and can control sliding glass doors, box both sides and rear portion are provided with 120 mesh nylon cloth, baffle is installed to box top and bottom, place 1 humidity control device on the baffle, the box is fixed with the nylon wire, nylon wire and box top distance 4cm, be provided with 1 temperature, humidity detection device on the box inboard bottom baffle, wherein breeds device still includes host egg's carrier medium, clip and bee device.
The bearing medium is non-woven fabrics, the bee breeding device is a three-dimensional plastic box, the bottom of the three-dimensional plastic box is provided with 2-3 layers (the thickness of each 2 layers is 0.1 mm) of moist roll paper with 10-12% of wettability, the top of the three-dimensional plastic box is provided with 6-8 ventilation pores, the aperture of each ventilation pore is 0.8-1.2 mm, the humidity control device is a moist foam box with the diameter of 10cm and the height of 4.8cm, and the moisture content of the moist foam is 10-15%.
Comparative example 1
Referring to the prior patent name of a breeding device for the black bees of the noctuid, the patent number is CN20212553959.6, the authorized public number is CN216254809U, a drawer-type breeding box with the specification of length, width, height=30 cm, 30cm and 20cm is manufactured indoors, and glass and a partition plate are added at the periphery and the top to form the breeding box for the adult black bees of the noctuid. The breeding environment conditions of the reference example 2 are that the illumination time is 15:9, the temperature is 26 ℃, and the air humidity is 60% -70%, and breeding is carried out in a drawer type breeding box. The number of dead insects, the collection number of parasitic egg masses, the survival, the number of parasitic and eclosion of the adult noctuid black egg bees and the like are recorded in daily observation. The conventional adult breeding method of the black wasps for noctuid using the drawer-type breeding box according to this comparative example was the same as that of example 2 except that the adult breeding box for noctuid black wasps was different from that of example 2.
10000 adult bees of the black-egg bees of the noctuid bred in the examples 1-3 and the comparative example 1 are taken, 3 repeats are set, the life span of the adult bees, the spawning quantity of the adult bees and the emergence rate of the adult bees are counted, then 10000 eggs are randomly taken from the eggs of each example and the comparative example on the same day, 3 repeats are set, the eggs are put into a plastic box for development until the adult bees emerge, and the development days of the eggs, the emergence rate of the bees and the emergence rate of the adult bees are counted. Finally, the breeding effects of examples 1 to 3 and comparative example 1 were evaluated by using 4 indexes of adult bee life, development calendar period of bees, bee parasitism rate and adult bee emergence rate, and the results are shown in Table 1.
The specific calculation standards of the life span, development calendar period, parasitic rate and emergence rate of the adult bees are as follows:
adult bee life: is the time between emergence and death of the adult bees.
Development calendar of bees: the time from the parasitic spawning of the bees to the emergence of the adult bees.
Parasitic ratio of bees = number of eggs of spodoptera frugiperda being parasitized/total number of eggs of spodoptera frugiperda
Adult bee emergence rate: the number of emergence heads of bees is the percentage of the number of parasitic eggs.
TABLE 1 effects of oviposition and breeding of Spodoptera frugiperda black wasps of examples 1 to 3 and comparative example 1
As can be seen from the results in Table 1, the adult life, development duration, parasitic rate and adult emergence rate of the black bees of the noctuid bred in the breeding method of the invention are significantly higher than those of the black bees of the comparative example 1, and the noctuid black bees are bred by the autonomous design of the noctuid black bees breeding device and the organic combination of temperature, humidity and photoperiod of breeding conditions, so that the proper breeding time is mastered, the breeding period is short, and the artificial cost is reduced by combining with the scientific breeding method, and the parasitic rate and adult emergence rate of the bees are improved. In the aspect of exploring the device and the method for breeding the noctuid black egg bees, the problem that the original noctuid black egg bees are difficult to breed in a large scale is gradually broken, so that the noctuid black egg bees can breed in a large scale.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. The utility model provides a device is bred to night moth black egg bee, includes box (1), its characterized in that, box (1) both sides and rear portion cladding have nylon cloth (2), box (1) front portion is equipped with chamber door (3), white light lamp (4) are installed to box (1) top inboard, baffle (5) are installed at box (1) top and bottom, humidity control device (6) have been placed on baffle (5), box (1) is fixed with nylon wire (7), nylon wire (7) are apart from 3 ~ 5cm with box (1) top.
2. The breeding device according to claim 1, characterized in that it further comprises a carrier medium (8) for the host eggs and a bee breeding device (9).
3. The breeding device according to claim 1, characterized in that the box (1) is provided with temperature and humidity detection means (10).
4. The breeding device according to claim 1, characterized in that the door (3) is a glass door; the specification of the white light lamp (4) is T5-12W/0.9 m; the humidity control device (6) is a wet foam box, and the moisture content of the wet foam is 10% -15%.
5. The breeding device according to claim 2, characterized in that the carrier medium (8) comprises a nonwoven fabric, the nonwoven fabric being white in size, 30g/m, 120cm in length, 40cm wide and 0.05mm thick; the bee breeding device (9) is a three-dimensional plastic box, moist roll paper with the moist degree of 10% -12% is placed at the bottom of the three-dimensional plastic box, 6-8 ventilation small holes are formed in the top of the three-dimensional plastic box, and the aperture of each ventilation small hole is 0.8-1.2 mm.
6. A method for breeding black wasps of noctuid by using the breeding device of any one of claims 1 to 5, comprising the steps of:
suspending a bearing medium containing 8% -10% of honey water on a nylon wire, putting the spodoptera exigua adult wasp and spodoptera exigua egg cloth in a breeding device, suspending the spodoptera exigua egg cloth on the nylon wire, carrying out spawning parasitic of the spodoptera exigua adult wasp, taking out the spodoptera exigua egg cloth which has been parasitized by the spodoptera exigua adult wasp after 24-48 h, then putting the spodoptera exigua egg cloth in a breeding device, and finally carrying out adult wasp breeding in the breeding device.
7. The method of claim 6, wherein the ratio of the adult wasps of the black wasps of the noctuid to the wasps of the spodoptera frugiperda is 1:30-50.
8. The method of claim 6, wherein the breeding conditions for the oviposition parasitic are: the illumination L is that D=14-16:10-8, the temperature is 26-28 ℃, and the air humidity is 60-70%.
9. The method of claim 6, wherein the conditions for adult bee breeding are: the temperature is 26-28 ℃, the illumination L is D=14-16:10-8, the air humidity is 60-70%, and the breeding time is 9-11D.
10. A method according to claim 6, wherein the spodoptera frugiperda eggs are spodoptera frugiperda eggs within 24-48 hours of the adult spodoptera frugiperda production.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116349656A (en) * | 2023-05-26 | 2023-06-30 | 云南省烟草公司昆明市公司 | Method for large-scale propagation of noctuid black egg bees |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116349656A (en) * | 2023-05-26 | 2023-06-30 | 云南省烟草公司昆明市公司 | Method for large-scale propagation of noctuid black egg bees |
| CN116349656B (en) * | 2023-05-26 | 2023-08-22 | 云南省烟草公司昆明市公司 | Method for large-scale propagation of noctuid black egg bees |
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