CN116119321B - Nucleic acid fluorescence detection equipment - Google Patents
Nucleic acid fluorescence detection equipment Download PDFInfo
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- CN116119321B CN116119321B CN202211686421.5A CN202211686421A CN116119321B CN 116119321 B CN116119321 B CN 116119321B CN 202211686421 A CN202211686421 A CN 202211686421A CN 116119321 B CN116119321 B CN 116119321B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/74—Feeding, transfer, or discharging devices of particular kinds or types
- B65G47/80—Turntables carrying articles or materials to be transferred, e.g. combined with ploughs or scrapers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/22—Devices influencing the relative position or the attitude of articles during transit by conveyors
- B65G47/24—Devices influencing the relative position or the attitude of articles during transit by conveyors orientating the articles
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/74—Feeding, transfer, or discharging devices of particular kinds or types
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65G—TRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
- B65G47/00—Article or material-handling devices associated with conveyors; Methods employing such devices
- B65G47/74—Feeding, transfer, or discharging devices of particular kinds or types
- B65G47/82—Rotary or reciprocating members for direct action on articles or materials, e.g. pushers, rakes, shovels
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Mechanical Engineering (AREA)
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- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
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- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a nucleic acid fluorescence detection device, comprising: the automatic feeding device comprises a working table, wherein a center column is fixedly connected to the top of the working table, a steering wheel is rotatably connected to the upper portion of the outer surface of the center column, a plurality of moving grooves are formed in the outer surface of the steering wheel in an annular equidistant manner, two long strips are fixedly connected to one side of the top of the working table, a feeding assembly is arranged between the adjacent sides of the long strips, a connecting rod and a first accelerating fluted disc are driven to rotate through a transmission worm wheel, so that the transmission belt rolls to drive a plurality of feeding assemblies to move, meanwhile, a transmission worm drives the steering wheel intermittently through intermittent bevel gears and a conical toothed ring, when the steering wheel intermittently rotates and stops, a test tube intermittently rotates through the inside of the moving grooves clamped by a feeding clamping groove of the connecting block, and detection time is reserved under the test tube intermittently rotating to the fluorescent detector, so that automatic feeding operation can be realized, the feeding efficiency is improved, and the detection efficiency is improved.
Description
Technical Field
The invention relates to the technical field of nucleic acid fluorescence detection, in particular to nucleic acid fluorescence detection equipment.
Background
The substance for nucleic acid detection is viral nucleic acid, and the nucleic acid detection is to find whether the human respiratory tract specimen, blood or feces is infected by the new coronavirus. Therefore, once the detection is positive, the existence of virus in a patient can be proved, after the new coronavirus infects the human body, the new coronavirus can be propagated in the respiratory tract system, so that the detection of the virus nucleic acid in sputum and nasopharyngeal swab can be used for judging whether the human body infects the virus, so that the nucleic acid detection positive can be used as a new standard for confirming the infection of the new coronavirus, the nucleic acid detection method is a method for judging whether the virus infects the virus by searching whether the DNA and RNA of the virus invaded by the outside exist in respiratory tract specimens, blood or feces of the patient, and the new coronavirus nucleic acid detection in the market at present mainly uses a fluorescent quantitative RT-PCR technology, which is a novel technology, and the technology is a combination of the fluorescent quantitative PCR technology and the RT-PCR technology. If a new coronavirus nucleic acid is present, the instrument can detect the fluorescent signal, and the fluorescent signal is continuously enhanced along with the continuous replication of DNA, so that the existence of the new coronavirus is indirectly detected, and a nucleic acid fluorescent detection device is usually required to be used for detection in the current nucleic acid detection.
However, the current nucleic acid fluorescence detection device can detect samples, but the operations of feeding, discharging, placing and the like all need manual work to operate in the detection process, and a lot of time is needed, so when a lot of nucleic acid detection is faced, a lot of manpower is needed to operate and detect, so the automation degree of the current nucleic acid fluorescence detection device is extremely low, the efficiency of manual operation detection is extremely low, and the practicability of the current nucleic acid fluorescence detection device on the market is low.
Disclosure of Invention
The present invention aims to solve one of the technical problems existing in the prior art or related technologies.
The technical scheme adopted by the invention is as follows:
a nucleic acid fluorescence detection apparatus comprising: the operation panel, operation panel top fixedly connected with center post, the upper portion of center post surface rotates and is connected with the steering wheel, the outward appearance of steering wheel is annular equidistance and leaves and be equipped with a plurality of removal grooves, one side fixedly connected with two long laths at operation panel top, two be provided with the material loading subassembly between the adjacent one side of long lath, two be provided with drive assembly between the both ends of long lath, two be provided with acceleration subassembly between the long lath, acceleration subassembly is connected with material loading subassembly and drive assembly, be provided with intermittent type subassembly between the bottom of steering wheel and the drive assembly, the center post top is provided with the unloading subassembly, the top of operation panel is provided with two and puts the subassembly, two put the subassembly respectively with two wherein remove the groove is corresponding, two put the subassembly, a plurality of the inside of removal groove and the material loading subassembly on all be provided with and put the material subassembly, the surface fixedly connected with support frame of operation panel, install fluorescence detector on the support frame, the surface of center post is provided with mark subassembly, mark subassembly and fluorescence detector and be located the below of fluorescence electrical property.
Preferably, the material loading subassembly includes two parallel bull sticks, two belt driving rollers, driving belt and a plurality of thing marking pad of putting, two parallel bull stick all rotates to be connected between the inside of two laths, two belt driving rollers fixed connection respectively at the surface of two parallel bull sticks, driving belt transmission is connected between the surface of two belt driving rollers, a plurality of put thing marking pad is annular equidistance fixed connection at driving belt's surface.
Preferably, the drive assembly comprises two mounting plates, a transmission worm and a driving motor, wherein the two mounting plates are respectively and fixedly connected to two ends of the long strip plate, the transmission worm is rotationally connected between the two mounting plates, one end of the transmission worm penetrates through one mounting plate, the driving motor is fixedly mounted on the other mounting plate, and an output shaft of the driving motor is fixedly connected with the other end of the transmission worm.
Preferably, the accelerating assembly comprises a connecting rod, a transmission worm gear, a first accelerating fluted disc, a gear column, a second accelerating fluted disc and a pinion, wherein the connecting rod is rotationally connected between one adjacent surfaces of two long strips, one end of the connecting rod penetrates through one of the long strips, the transmission worm gear is fixedly sleeved on the connecting rod, the transmission worm gear is meshed with the transmission worm, the first accelerating fluted disc is fixedly connected with one end of the connecting rod, the gear column is rotationally connected with the front end surface of one of the long strips, the gear column is meshed with the first accelerating fluted disc, the second accelerating fluted disc is fixedly connected with the front end surface of the gear column, the pinion is fixedly connected with one end of one of the parallel rotating rods, and the pinion is meshed with the second accelerating fluted disc.
Preferably, the intermittent assembly comprises intermittent bevel gears, four vertical rods and a conical toothed ring, wherein the intermittent bevel gears are fixedly connected with one end of the transmission worm, the four vertical rods are fixedly connected to the bottom of the steering wheel, the conical toothed ring is fixedly connected between the bottom ends of the four intermittent bevel gears, and the conical toothed ring is meshed with the intermittent bevel gears.
Preferably, the material subassembly of putting includes supporting disk, test tube and linking piece, the supporting disk sets up at the top of one of them thing mark pad of putting, test tube fixed connection is at the top of supporting disk, linking piece fixed connection is at the surface of test tube, the material loading draw-in groove has been seted up to the surface of linking piece, material loading draw-in groove and a plurality of removal groove looks adaptations.
Preferably, the unloading subassembly includes mounting groove, driving motor, disc, two vertical poles, two cavity pieces, cavity reciprocal slide and L shape push rod, the top at the center post is seted up to the mounting groove, driving motor fixed mounting is in the inside of mounting groove, disc fixed connection is at driving motor's output shaft, the top fixed connection of disc is at the sliding shaft, two the equal fixed connection of vertical poles is at the top of center post, two the cavity piece is fixed connection respectively at the top of two vertical poles, two the reciprocal groove has all been seted up to the adjacent one side of cavity piece, the reciprocal slide sliding connection of cavity is between the mutually inner wall of two reciprocal grooves, L shape push rod fixed connection is in one side of cavity reciprocal slide, sliding shaft sliding connection is in the inside of cavity reciprocal slide.
Preferably, the placing assembly comprises two hollow frames and two supporting sliding sheets, wherein the two hollow frames are fixedly connected to the top of the operation table, the two supporting sliding sheets are respectively arranged between one surfaces of the two supporting sliding sheets, which are opposite to each other, the two supporting sliding sheets are matched with the feeding clamping groove, and the two supporting sliding sheets are matched with one of the moving grooves.
Preferably, the marking assembly comprises a reinforcing plate, two mounting seats, an air cylinder, a pushing plate and a marking stamp, wherein the reinforcing plate is fixedly connected to the outer surface of the center column, the two mounting seats are fixedly connected to the top of the reinforcing plate, the air cylinder is fixedly arranged between the interiors of the two mounting seats, the pushing plate is fixedly connected with an output shaft of the air cylinder, the marking stamp is fixedly connected with the pushing plate, and the air cylinder is electrically connected with the fluorescence detector.
Preferably, the centering assembly comprises a linkage worm, two linking rollers, a linking belt, a steel structure plate, a steel structure shaft, a linkage worm wheel and a centering wheel, wherein the linkage worm is rotationally connected to one of the long strips, the two linking rollers are respectively and fixedly connected to one end of the other parallel rotating rod and the outer surface of the linkage worm, the linking belt is in transmission connection with the outer surfaces of the two linking rollers, the steel structure plate is arranged at the top of one of the long strips, the steel structure shaft is rotationally connected to the inside of the steel structure plate, the linkage worm wheel is fixedly connected to the bottom end of the steel structure shaft and meshed with the linkage worm, and the centering wheel is fixedly connected to the top end of the steel structure shaft.
By adopting the technical scheme, the beneficial effects obtained by the invention are as follows:
1. according to the invention, the drive motor is started to drive the transmission worm to rotate, the connecting rod and the first accelerating fluted disc are driven to rotate through the transmission worm wheel, and then the parallel rotating rods and the belt driving roller are driven to rotate through the gear column, the second accelerating fluted disc and the pinion in an accelerating way, so that the transmission belt rolls to drive the plurality of material placing components to move, meanwhile, the transmission worm intermittently drives the steering wheel through the intermittent bevel gear and the conical toothed ring, when the steering wheel intermittently rotates and stops, the test tube is clamped into the interior of the moving groove to intermittently rotate through the material loading clamping groove of the connecting block, and the test tube intermittently rotates to the lower part of the fluorescence detector to reserve the detection time, so that the detection of samples is facilitated, the material loading operation can be automated, the material loading efficiency is improved, and the detection efficiency is improved.
2. According to the invention, the disc is driven to rotate by opening the transmission motor, the disc slides in the hollow reciprocating slide plate through the sliding shaft and pushes the hollow reciprocating slide plate to swing back and forth between the two hollow blocks, so that the L-shaped push rod can push the detection non-abnormal material placing component to be placed between the other two supporting slide plates, the detected test tube can swing in order, the subsequent uniform treatment of the detection non-abnormal sample by workers is facilitated, the automatic blanking is realized, and the practicability of the device is improved.
3. According to the invention, the cylinder is opened through the fluorescence detector to drive the push plate to move, the push plate pushes the test tube to be clamped between the two supporting sliding sheets through the feeding clamping groove, and the test tube is contacted with the marking stamp to mark the test tube, so that the test tube is convenient for staff to distinguish, the automatic operation on the abnormal sample can be finished, and the practicability of the device is further improved.
4. The linkage worm is driven through the two connecting rollers and the connecting belt, the linkage worm drives the steel structure shaft through the linkage worm wheel, the steel structure shaft drives the righting wheel to rotate, and the righting wheel can righting the test tube when rotating so as to avoid the skew of the test tube from affecting feeding.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic overall sectional view of the present invention;
FIG. 3 is a schematic view of an exploded view of the drive assembly and the feed assembly of the present invention;
FIG. 4 is an enlarged schematic view of the structure of the present invention at A;
FIG. 5 is a schematic view of the intermittent assembly of the present invention;
FIG. 6 is a schematic diagram of the structure of the blanking and placement components of the present invention;
FIG. 7 is a schematic view of a placement module according to the present invention;
FIG. 8 is an enlarged schematic view of the structure of the present invention at B;
fig. 9 is a schematic structural view of the aligning member of the present invention.
Reference numerals:
1. a work table; 2. a feeding assembly; 201. a parallel rotating rod; 202. a belt driving roller; 203. a drive belt; 204. placing object marking pads; 3. a drive assembly; 301. a mounting plate; 302. a drive worm; 303. a driving motor; 4. an acceleration assembly; 401. a connecting rod; 402. a drive worm wheel; 403. a first accelerating fluted disc; 404. a gear post; 405. a second accelerating fluted disc; 406. a pinion gear; 5. an intermittent assembly; 501. intermittent bevel teeth; 502. a vertical rod; 503. conical toothed ring; 6. a blanking assembly; 601. a mounting groove; 602. a drive motor; 603. a disc; 604. a vertical rod; 605. a hollow block; 606. a hollow reciprocating slide plate; 607. an L-shaped push rod; 7. placing the components; 701. a hollow frame; 702. supporting a sliding sheet; 8. a material placing component; 801. a support plate; 802. a test tube; 803. a joint block; 9. a marking assembly; 901. a reinforcing plate; 902. a mounting base; 903. a cylinder; 904. a push plate; 905. marking a seal; 10. a long slat; 11. a center column; 12. a steering wheel; 13. a support frame; 14. a fluorescence detector; 15. a centering component; 16. a linkage worm; 17. two engagement rollers; 18. a connecting belt; 19. a steel structural plate; 20. a steel structure shaft; 21. a linked worm wheel; 22. pushing the correcting wheel.
Detailed Description
The objects, technical solutions and advantages of the present invention will become more apparent by the following detailed description of the present invention with reference to the accompanying drawings. It should be noted that, without conflict, the embodiments of the present invention and features in the embodiments may be combined with each other.
It is to be understood that this description is merely exemplary in nature and is not intended to limit the scope of the present invention.
A nucleic acid fluorescence detection apparatus provided by some embodiments of the present invention is described below with reference to the accompanying drawings.
Embodiment one:
referring to FIGS. 1 to 9, the present invention provides a nucleic acid fluorescence detection apparatus comprising: the automatic fluorescence detection device comprises an operation table 1, a center column 11 is fixedly connected to the top of the operation table 1, a steering wheel 12 is rotatably connected to the upper portion of the outer surface of the center column 11, a plurality of moving grooves are formed in the outer surface of the steering wheel 12 in an annular equidistant manner, two long strips 10 are fixedly connected to one side of the top of the operation table 1, a feeding component 2 is arranged between the adjacent faces of the two long strips 10, a driving component 3 is arranged between the two ends of the two long strips 10, an accelerating component 4 is arranged between the two long strips 10, the accelerating component 4 is connected with the feeding component 2 and the driving component 3, an intermittent component 5 is arranged between the bottom of the steering wheel 12 and the driving component 3, a blanking component 6 is arranged at the top of the center column 11, two placing components 7 are respectively corresponding to the two moving grooves, a fluorescence detector 14 is arranged on the two placing components 7, the inner parts of the plurality of the moving grooves and the feeding component 2, a fluorescence detector 14 is fixedly connected to the outer surface of the operation table 1, a marking component 9 is arranged on the supporting frame 13, and the outer surface of the center column 11 is provided with a marking component 9, and the marking component 9 is electrically connected with the fluorescence detector 14 below the marking component 14.
When using this equipment, the inside of putting material subassembly 8 will place the sample that nucleic acid detected, afterwards the staff will need to detect put material subassembly 8 unified place on material subassembly 2, afterwards the staff will open drive assembly 3, drive assembly 3 will accelerate subassembly 4 and drive material subassembly 2 and put material subassembly 8 and remove, drive assembly 3 will carry out intermittent type transmission through intermittent type subassembly 5 transmission steering wheel 12 simultaneously, when steering wheel 12 intermittent type rotates to stop, the material subassembly 8 of putting on the material subassembly 2 will go into the inside of removal groove with the material card, drive and put material subassembly 8 intermittent type rotation and also can be convenient for the detection to the sample, thereby let the material loading operation can realize automaticly, promote material loading efficiency, and promote detection efficiency. The continuous intermittent rotation of the subsequent steering wheel 12 drives the material placing component 8 to move to the bottom of the fluorescence detector 14 for fluorescence detection, the abnormal material placing component 8 after detection is pushed to one of the placing components 7 by the marking component 9 and marked, the non-abnormal material placing component 8 continues to intermittently rotate, and the abnormal material placing component 6 is pushed to the other placing component 7, so that the detected material placing component 8 can swing neatly, and the staff can recognize conveniently.
Embodiment two:
referring to fig. 1 to 9, on the basis of the first embodiment, the feeding assembly 2 includes two parallel rotating rods 201, two belt driving rollers 202, a driving belt 203 and a plurality of object placing marking pads 204, the two parallel rotating rods 201 are rotatably connected between the interiors of the two long strips 10, the two belt driving rollers 202 are fixedly connected to the outer surfaces of the two parallel rotating rods 201 respectively, the driving belt 203 is in transmission connection between the outer surfaces of the two belt driving rollers 202, the plurality of object placing marking pads 204 are fixedly connected to the outer surfaces of the driving belt 203 in an annular equidistant manner, the driving assembly 3 includes two mounting plates 301, a driving worm 302 and a driving motor 303, the two mounting plates 301 are fixedly connected to the two ends of the long strips 10 respectively, the driving worm 302 is rotatably connected between the two mounting plates 301, one end of the driving worm 302 penetrates one mounting plate 301, the driving motor 303 is fixedly arranged on the other mounting plate 301, the output shaft of the driving motor 303 is fixedly connected with the other end of the driving worm 302, the accelerating assembly 4 comprises a connecting rod 401, a driving worm wheel 402, a first accelerating fluted disc 403, a gear column 404, a second accelerating fluted disc 405 and a pinion 406, the connecting rod 401 is rotationally connected between the adjacent surfaces of two long strips 10, one end of the connecting rod 401 penetrates through one of the long strips 10, the driving worm wheel 402 is fixedly sleeved on the connecting rod 401, the driving worm wheel 402 is meshed with the driving worm 302, the first accelerating fluted disc 403 is fixedly connected with one end of the connecting rod 401, the gear column 404 is rotationally connected with the front end surface of one of the long strips 10, the gear column 404 is meshed with the first accelerating fluted disc 403, the second accelerating fluted disc 405 is fixedly connected with the front end surface of the gear column 404, the pinion 406 is fixedly connected with one end of one of the parallel rotating rods 201, pinion 406 is meshed with a second accelerating toothed disc 405.
Embodiment III:
in the above embodiment, as shown in fig. 1 to 9, the blanking assembly 6 includes a mounting groove 601, a transmission motor 602, a disc 603, two vertical rods 604, two hollow blocks 605, a hollow reciprocating slide plate 606 and an L-shaped push rod 607, the mounting groove 601 is formed at the top of the central column 11, the transmission motor 602 is fixedly installed in the mounting groove 601, the disc 603 is fixedly connected to the output shaft of the transmission motor 602, the top of the disc 603 is fixedly connected to a sliding shaft, the two vertical rods 604 are fixedly connected to the top of the central column 11, the two hollow blocks 605 are respectively and fixedly connected to the top of the two vertical rods 604, the adjacent surfaces of the two hollow blocks 605 are respectively formed with a reciprocating groove, the hollow reciprocating slide plate 606 is slidably connected between the inner walls of the two reciprocating grooves, the L-shaped push rod 607 is fixedly connected to one side of the hollow reciprocating slide plate 606, the sliding shaft is slidably connected to the inside of the hollow reciprocating slide plate 606, the placing assembly 7 includes two hollow frames 701 and two supporting sliding vanes 701, the two hollow frames are fixedly connected to the top of the operation table 1, the two supporting sliding vanes 702 are respectively matched with the two supporting sliding vanes 702, and the two supporting sliding vanes 702 are matched with the two supporting slots.
By opening the transmission motor 602, the transmission motor 602 drives the disc 603 to rotate, the disc 603 slides inside the hollow reciprocating slide plate 606 through the sliding shaft and pushes the hollow reciprocating slide plate 606 to swing reciprocally between the two hollow blocks 605, so that the L-shaped push rod 607 pushes the detection material placing component 8 without abnormality to be placed between the other two supporting slide plates 702, and the detected test tube 802 is subjected to regular swing.
Embodiment four:
referring to fig. 1 to 9, in the above embodiment, the marking assembly 9 includes a reinforcing plate 901, two mounting seats 902, an air cylinder 903, a push plate 904 and a marking stamp 905, the reinforcing plate 901 is fixedly connected to the outer surface of the center column 11, the two mounting seats 902 are all fixedly connected to the top of the reinforcing plate 901, the air cylinder 903 is fixedly mounted between the interiors of the two mounting seats 902, the push plate 904 is fixedly connected to the output shaft of the air cylinder 903, the marking stamp 905 is fixedly connected to the push plate 904, the air cylinder 903 is electrically connected to the fluorescence detector 14, the centering assembly 15 includes a linkage worm 16, two linkage rollers 17, a linkage belt 18, a steel structure plate 19, a steel structure shaft 20, a linkage worm wheel 21 and a pushing wheel 22, the linkage worm 16 is rotatably connected to one of the long plates 10, the two linkage rollers 17 are fixedly connected to one end of the other parallel rod 201 and the outer surface of the linkage worm 16, the linkage belt 18 is in transmission connection to the outer surface of the two linkage rollers 17, the steel structure plate 19 is mounted on the top of one of the long plates 10, the steel structure shaft 20 is rotatably connected to the interior of the steel structure plate 19, the linkage worm wheel 21 is fixedly connected to the bottom end of the steel structure shaft 20 and is fixedly meshed with the pushing wheel 22.
The cylinder 903 is opened through the fluorescence detector 14 to drive the push plate 904 to move, the push plate 904 pushes the test tube 802 to be clamped between the two supporting slide sheets 702 through the feeding clamping groove, the test tube 802 is contacted with the marking stamp 905 to be marked, staff is convenient to distinguish, the linkage worm 16 is driven through the two linkage rollers 17 and the linkage belt 18, the linkage worm 16 drives the steel structure shaft 20 through the linkage worm wheel 21, the steel structure shaft 20 drives the righting wheel 22 to rotate, and the test tube 802 is righted when the righting wheel 22 rotates to avoid the influence of the skew of the test tube on feeding.
The working principle and the using flow of the invention are as follows: when the device is used, a sample for detecting nucleic acid is placed in the test tube 802, then a worker correspondingly places the supporting disc 801 above the plurality of object placing marking pads 204 at equal intervals, then the worker turns on the driving motor 303, the driving motor 303 drives the driving worm 302 to rotate, the driving worm 302 drives the connecting rod 401 and the first accelerating fluted disc 403 to rotate through the driving worm wheel 402 when rotating, the first accelerating fluted disc 403 accelerates the driving gear column 404, the first accelerating fluted disc 403 drives the second accelerating fluted disc 405 to accelerate the driving pinion 406, the pinion 406 drives the parallel rotating rod 201 and the belt driving roller 202 to rotate, thereby the driving belt 203 rolls to drive the plurality of object placing components 8 to move, meanwhile, the driving worm 302 drives the tapered toothed ring 503 through the intermittent tapered conical teeth 501 intermittently, the tapered toothed ring 503 drives the steering wheel 12 through the four vertical rods 502 to intermittently drive, when the steering wheel 12 intermittently rotates and stops, the test tube 802 on the object placing marking pad 204 is clamped into the moving groove through the feeding clamping groove on the connecting block 803, so that the steering wheel 12 drives the test tube 802 to intermittently rotate, the test tube 802 intermittently rotates to the lower part of the fluorescence detector 14 to reserve detection time, further the detection of samples is facilitated, the feeding operation can be automated, the feeding efficiency is improved, the detection efficiency is improved, after the fluorescence detector 14 detects the abnormal test tube 802, the fluorescence detector 14 can open the air cylinder 903 to drive the push plate 904 to move, the push plate 904 pushes the test tube 802 to be clamped between the two supporting sliding sheets 702 through the feeding clamping groove, the test tube 802 is marked through the marking seal 905, the test tube 802 is conveniently distinguished by staff, the test tube 802 without abnormality continuously rotates intermittently, the transmission motor 602 is opened, the transmission motor 602 drives the disc 603 to rotate, the disc 603 slides in the hollow reciprocating slide plate 606 through the sliding shaft and pushes the hollow reciprocating slide plate 606 to swing back and forth between the two hollow blocks 605, the L-shaped push rod 607 pushes the detection material placing component 8 without abnormality to be placed between the other two supporting slide sheets 702, the detected test tube 802 is subjected to orderly swing, thereby being convenient for workers to uniformly process, the linkage worm 16 is driven through the two connecting rollers 17 and the connecting belt 18, the linkage worm 16 drives the steel structure shaft 20 through the linkage worm wheel 21, the steel structure shaft 20 drives the righting wheel 22 to rotate, and the test tube 802 is righted when the righting wheel 22 rotates to avoid the influence of the skew of the test tube on feeding.
In the present invention, the term "plurality" means two or more, unless explicitly defined otherwise. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items. The terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; "coupled" may be directly coupled or indirectly coupled through intermediaries. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
It will be understood that when an element is referred to as being "mounted," "secured" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.
In the description of the present specification, the terms "one embodiment," "some embodiments," "particular embodiments," and the like, mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the spirit and scope of the invention as defined by the appended claims and their equivalents.
Claims (7)
1. A nucleic acid fluorescence detection apparatus, characterized by comprising: the utility model provides a workbench (1), workstation (1) top fixedly connected with center post (11), the upper portion of center post (11) surface rotates and is connected with steering wheel (12), the outward appearance of steering wheel (12) is annular equidistance and leaves and be equipped with a plurality of removal grooves, one side fixedly connected with two slat (10) at workstation (1) top, two be provided with material loading subassembly (2) between the one side that slat (10) are adjacent, two be provided with drive assembly (3) between the both ends of slat (10), two be provided with between slat (10) accelerating assembly (4), accelerating assembly (4) are connected with material loading subassembly (2) and drive assembly (3), be provided with intermittent type subassembly (5) between the bottom of steering wheel (12) and drive assembly (3), center post (11) top is provided with unloading subassembly (6), the top of workstation (1) is provided with two subassemblies (7), two put subassembly (7) respectively with two wherein remove the groove, two subassembly (7) are located respectively, two the inside subassembly (7) are located on the surface of the support frame (1) and are located on the fixed outside of the platform (1), the fluorescent detector (14) is mounted on the support frame (13), the marking component (9) is arranged on the outer surface of the center column (11), the marking component (9) is electrically connected with the fluorescent detector (14) and is positioned below the fluorescent detector (14), the opposite surfaces of the two long strips (10) are respectively provided with a straightening component (15), and the two straightening components (15) are connected with the feeding component (2); the driving assembly (3) comprises two mounting plates (301), a transmission worm (302) and a driving motor (303), wherein the two mounting plates (301) are respectively and fixedly connected to two ends of the long strip plate (10), the transmission worm (302) is rotationally connected between the two mounting plates (301), one end of the transmission worm (302) penetrates through one mounting plate (301), the driving motor (303) is fixedly arranged on the other mounting plate (301), and an output shaft of the driving motor (303) is fixedly connected with the other end of the transmission worm (302); the feeding assembly (2) comprises two parallel rotating rods (201), two belt driving rollers (202), a driving belt (203) and a plurality of object placing marking pads (204), wherein the two parallel rotating rods (201) are rotationally connected between the interiors of the two long strips (10), the two belt driving rollers (202) are respectively and fixedly connected to the outer surfaces of the two parallel rotating rods (201), the driving belt (203) is in driving connection between the outer surfaces of the two belt driving rollers (202), and the object placing marking pads (204) are fixedly connected to the outer surfaces of the driving belt (203) at equal intervals in an annular shape; the accelerating assembly (4) comprises a connecting rod (401), a transmission worm wheel (402), a first accelerating fluted disc (403), a gear column (404), a second accelerating fluted disc (405) and a pinion (406), wherein the connecting rod (401) is rotationally connected between two adjacent surfaces of the long fluted disc (10), one end of the connecting rod (401) penetrates through one of the long fluted disc (10), the transmission worm wheel (402) is fixedly sleeved on the connecting rod (401), the transmission worm wheel (402) is meshed with the transmission worm (302), the first accelerating fluted disc (403) is fixedly connected to one end of the connecting rod (401), the gear column (404) is rotationally connected to the front end surface of one of the long fluted disc (10), the gear column (404) is meshed with the first accelerating fluted disc (403), the second accelerating fluted disc (405) is fixedly connected to the front end surface of the gear column (404), and the pinion (406) is fixedly connected with one end of one of the parallel rotating rod (201), and the pinion (406) is meshed with the second accelerating fluted disc (405).
2. The nucleic acid fluorescence detection apparatus according to claim 1, wherein the intermittent assembly (5) includes intermittent bevel teeth (501), four vertical rods (502) and a tapered toothed ring (503), the intermittent bevel teeth (501) are fixedly connected with one end of the worm gear (302), the four vertical rods (502) are fixedly connected with the bottom of the steering wheel (12), the tapered toothed ring (503) is fixedly connected between the bottom ends of the four intermittent bevel teeth (501), and the tapered toothed ring (503) is meshed with the intermittent bevel teeth (501).
3. The nucleic acid fluorescence detection device according to claim 1, wherein the material placing component (8) comprises a supporting disc (801), a test tube (802) and a connecting block (803), the supporting disc (801) is arranged at the top of one of the material placing marking pads (204), the test tube (802) is fixedly connected to the top of the supporting disc (801), the connecting block (803) is fixedly connected to the outer surface of the test tube (802), a feeding clamping groove is formed in the outer surface of the connecting block (803), and the feeding clamping groove is matched with the plurality of moving grooves.
4. A nucleic acid fluorescence detection apparatus according to claim 3, wherein the blanking component (6) comprises a mounting groove (601), a transmission motor (602), a disc (603), two vertical rods (604), two hollow blocks (605), a hollow reciprocating slide plate (606) and an L-shaped push rod (607), the mounting groove (601) is formed at the top of the central column (11), the transmission motor (602) is fixedly mounted in the mounting groove (601), the disc (603) is fixedly connected to an output shaft of the transmission motor (602), the top of the disc (603) is fixedly connected to a sliding shaft, two vertical rods (604) are fixedly connected to the top of the central column (11), two hollow blocks (605) are respectively fixedly connected to the top ends of the two vertical rods (604), the adjacent surfaces of the hollow blocks (605) are respectively provided with a reciprocating groove, the hollow reciprocating slide plate (606) is slidingly connected between the inner walls of the two reciprocating groove phases, the L-shaped push rod (607) is fixedly connected to the sliding shaft of the hollow reciprocating slide plate (606), and the hollow reciprocating slide plate (606) is slidingly connected to the inner side of the hollow slide plate (606).
5. The nucleic acid fluorescence detection device according to claim 1, wherein the placement component (7) comprises two hollow frames (701) and two supporting sliding sheets (702), the two hollow frames (701) are fixedly connected to the top of the workbench (1), the two supporting sliding sheets (702) are respectively arranged between one surfaces of the two supporting sliding sheets (702) opposite to each other, the two supporting sliding sheets (702) are matched with the feeding clamping grooves, and the two supporting sliding sheets (702) are matched with one of the moving grooves.
6. The nucleic acid fluorescence detection apparatus according to claim 1, wherein the marking assembly (9) comprises a reinforcing plate (901), two mounting seats (902), an air cylinder (903), a push plate (904) and a marking stamp (905), the reinforcing plate (901) is fixedly connected to the outer surface of the center column (11), the two mounting seats (902) are fixedly connected to the top of the reinforcing plate (901), the air cylinder (903) is fixedly mounted between the interiors of the two mounting seats (902), the push plate (904) is fixedly connected to an output shaft of the air cylinder (903), the marking stamp (905) is fixedly connected to the push plate (904), and the air cylinder (903) is electrically connected to the fluorescence detector (14).
7. The nucleic acid fluorescence detection device according to claim 1, wherein the aligning assembly (15) comprises a linkage worm (16), two connecting rollers (17), a connecting belt (18), a steel structure plate (19), a steel structure shaft (20), a linkage worm wheel (21) and a pushing wheel (22), the linkage worm (16) is rotationally connected to one of the long plates (10), two connecting rollers (17) are respectively and fixedly connected to one end of another parallel rotating rod (201) and the outer surface of the linkage worm (16), the connecting belt (18) is in transmission connection with the outer surface of the two connecting rollers (17), the steel structure plate (19) is installed at the top of one of the long plates (10), the steel structure shaft (20) is rotationally connected to the inside of the steel structure plate (19), the linkage worm wheel (21) is fixedly connected to the bottom end of the steel structure shaft (20) and meshed with the linkage worm, and the pushing wheel (22) is fixedly connected to the top end of the steel structure shaft (20).
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| KR950001819B1 (en) * | 1992-11-13 | 1995-03-03 | 조광호 | Transfer device for stacking sacks |
| KR100695148B1 (en) * | 2005-05-02 | 2007-03-14 | 삼성전자주식회사 | Module for polymerase chain reaction and multiple polymerase chain reaction system |
| JP2007265506A (en) * | 2006-03-28 | 2007-10-11 | Funai Electric Co Ltd | Optical disk device provided with fluorescence detection function, and optical disk |
| KR101053895B1 (en) * | 2007-12-24 | 2011-08-04 | (주)나노스토리지 | Biochip Detection Device |
| CN204918592U (en) * | 2015-09-09 | 2015-12-30 | 武汉科技大学 | Semi -automatic and can rectify PCR fluorescence detector |
| CN106198480B (en) * | 2016-08-30 | 2018-09-14 | 广州蓝勃生物科技有限公司 | A kind of automatic dry type fluorescence detector |
| TWI833707B (en) * | 2017-09-21 | 2024-03-01 | 澳大利亞商英特葛雷特塑膠公司 | A continuously rotating, non-symmetric preform feed, stretch-blow-moulding machine and the related method |
| CN212127095U (en) * | 2019-12-30 | 2020-12-11 | 天津市万海电力成套设备有限公司 | Cable recovery coiling mechanism for power engineering |
| CN111646176B (en) * | 2020-06-12 | 2021-09-10 | 安徽蕴辉科技有限公司 | Printing waste recycling process |
| CN213056468U (en) * | 2020-08-31 | 2021-04-27 | 扬州云伟机电设备有限公司 | Machine parts processing is with surperficial mark device of beating |
| CN214454400U (en) * | 2020-12-29 | 2021-10-22 | 上海德惠制罐有限公司 | Industrial iron can pressing device |
| CN112895051A (en) * | 2021-02-23 | 2021-06-04 | 姜桃 | Flowerpot assembly device for landscaping |
| CN114161433B (en) * | 2021-04-28 | 2023-11-21 | 郑州轻工业大学 | Automatic marking robot and marking method after package security inspection |
| CN113479608B (en) * | 2021-09-06 | 2021-11-16 | 江苏众润光电材料科技有限公司 | Building safety glass intermediate film self-cutting conveying device |
| CN115303788A (en) * | 2022-08-18 | 2022-11-08 | 深圳市日联科技有限公司 | Automatic go up battery testing device of unloading |
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