CN116114711A - Indoor environment microorganism cleaning agent and application thereof - Google Patents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/02—Acaricides
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/381—Microorganisms
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- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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Abstract
The invention discloses an indoor environment microorganism cleaner and application thereof, wherein the microorganism cleaner comprises bacillus subtilis (CCTCC NO: M2022266) and bacillus amyloliquefaciens (CCTCC NO: M2022265), and the application effects comprise the following aspects: (1) The agent has good inhibition effect on indoor pathogenic microorganisms, can effectively reduce the loading capacity of the indoor pathogenic microorganisms in different environments, and reduces the pathogenic risk; (2) has good security; (3) The indoor common allergens (house dust mite allergens, wool allergens and cockroach allergens) have good degradation effects, so that the allergy risk is effectively reduced; (4) Effectively inhibit the activity of dust mites and help to reduce the harm of indoor dust mites; (5) effectively removing formaldehyde and other harmful substances.
Description
Technical Field
The invention belongs to the technical field of indoor environment purification, and particularly relates to an indoor environment microorganism cleaner and application thereof.
Background
With the improvement of living standard, people pay attention to the quality of the home environment, and many consumers purchase indoor environment purification products in a dispute due to health consideration. However, environmental cleaners currently in the market are mainly chemical cleaners, which contain more chemical substances: such as bactericides, surfactants, and the like. The cleaning agents can effectively kill environmental pathogenic microorganisms after being used, but have the defects that the cleaning agents are easy to cause the drug resistance of the microorganisms after long-term use, and have certain risks for the health of people after long-term use, such as easy skin allergy and the like. In contrast, the biological agent does not contain any chemical harmful substances, is nontoxic and residue-free in use, is safe and reliable, and can even obtain multiple functions of cleaning through compounding.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an indoor environment microorganism cleaner, which contains probiotics, can effectively inhibit pathogenic microorganisms in the environment after being released to the environment, reduces the content of protein allergens in the environment and reduces the allergy risk.
In one aspect, the invention provides a microbial cleaner comprising a microbial cleaner having a preservation number of cctccc NO: bacillus subtilis (Bacillus subtilis) YHX J of M2022266 with a preservation number of CCTCC NO: m2022265 Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YHX002J.
In another aspect, the invention provides the use of the microbial cleaner described above for inhibiting indoor pathogenic microorganisms, including pathogenic microorganisms having hemolytic properties such as staphylococcus aureus, streptococcus hemolyticus, acinetobacter, and the like.
In another aspect, the invention provides the use of the microbial cleaner described above for degrading allergens, including dust mite allergens, house dust mite allergens, cat allergens, cockroach allergens.
In another aspect, the invention provides the use of a microbial cleaner as described above for inhibiting or killing dust mites.
The invention also protects the application of the microbial cleaner in formaldehyde removal.
Compared with the prior art, the invention has the following beneficial effects:
1. the microbial cleaner is prepared by compounding the bacillus subtilis and the bacillus amyloliquefaciens, can effectively inhibit the growth of pathogenic microorganisms in the indoor environment after being used, obviously reduces the load of the microbial cleaner, obviously reduces the allergen in the indoor environment after being used, reduces the allergy risk, and can also effectively inhibit the activity of dust mites and remove formaldehyde.
2. The indoor environment microorganism cleaner disclosed by the invention has the advantages of safety, no residue, difficulty in generating microorganism drug resistance, difficulty in causing adverse reactions of people and the like in terms of use, and can keep long-term clean after being continuously used.
Drawings
FIG. 1 shows the results of an experiment for inhibiting bacteria in an indoor environment microbial cleaner prepared in example 1.
Detailed Description
Example 1 preparation method of microbial detergent
Biological material: bacillus subtilis (Bacillus subtilis) YHX J with a preservation number of CCTCCNO: m2022266, the preservation date 2022, 3 and 15 days, the preservation unit is China center for type culture Collection, and the preservation address: chinese university of Wuhan; bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YHX002J with a preservation number of CCTCC NO: m2022265, the preservation date 2022, 3 and 15 days, the preservation unit is China center for type culture Collection, and the preservation address: chinese university of Wuhan and Wuhan.
(1) Preparation and counting of bacillus subtilis spore concentrate
Inoculating YHX J bacillus subtilis into a seed culture medium for activation culture until the logarithmic phase of growth to obtain activated thalli; inoculating the obtained activated thallus into liquid fermentation medium at 3-5% by volume, fermenting at 37deg.C for 18-24 hr to obtain bacillus subtilis spore fermentation liquid, washing the fermentation liquid, filtering with membrane, concentrating to obtain concentrated solution, counting to determine spore content, and detecting to obtain bacillus subtilis with concentration of 60×10 8 CFU/mL。
(2) Preparation and counting of bacillus amyloliquefaciens spore concentrate
Inoculating bacillus amyloliquefaciens YHX002J into a seed culture medium for activation culture until the logarithmic phase of growth to obtain activated thalli; inoculating the obtained activated thallus into liquid fermentation medium at 3-5% by volume, fermenting at 37deg.C for 16-22 hr to obtain Bacillus amyloliquefaciens spore fermentation liquid, washing the fermentation liquid, membrane filtering and concentrating to obtain concentrated solution, counting to determine spore content in the concentrated solution, and detecting to obtain Bacillus amyloliquefaciens with concentration of 100deg.C 10 8 CFU/mL。
The seed culture medium in the steps (1) and (2) comprises the following components in percentage by weight: yeast extract powder 0.5-1.0%, peptone 1-2%, sodium chloride 0.5-1%, glucose 1-2%, and pH 7.0-7.2.
The activation culture conditions in the steps (1) and (2) are as follows: inoculating the strain preserved by the flat plate into a seed culture medium, and culturing for 12-15 hours at 37 ℃ under 180 rpm shaking to obtain the activated thallus.
The liquid fermentation medium in the step (1) comprises the following components in percentage by weight: glucose 0.5%, peptone 1%, yeast extract 0.5%, potassium dihydrogen phosphate 0.1%, disodium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, manganese chloride 0.02%, calcium chloride 0.1%, and pH 7.0-7.2.
The liquid fermentation medium in the step (2) comprises the following components in percentage by weight: glucose 1%, peptone 1%, yeast extract 0.5%, potassium dihydrogen phosphate 0.1%, disodium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, manganese chloride 0.02%, calcium chloride 0.15%, and pH 6.8.
The method for counting spores is as follows: transferring 10ml of fermentation culture solution by a pipettor under aseptic condition, placing the fermentation culture solution into a 50ml aseptic triangular flask, carrying out heat treatment at 80+/-1 ℃ in water bath for 10min, fully shaking, placing 1ml of water bath sample into a glass test tube of 9ml of aseptic normal saline to prepare 1:10, after fully mixing, transferring 1ml of sample homogenate into a glass test tube of 9ml of sterile physiological saline, fully mixing, and preparing into a sample 1: 100. Bacterial suspensions with different dilution gradients are prepared by analogy. 3 appropriate dilutions were selected and 100. Mu.l each was pipetted with a sterile pipette and plated onto 2 nutrient agar plates in parallel. Placing the coated plate at room temperature for 15min to allow the inoculating solution to be absorbed by agar completely, inverting, culturing at 36+ -1deg.C for 48+ -2 hr, selecting plate count with colony count of 30-300cfu, and calculating spore content. Nutrient agar plates were prepared as follows: 10.0g/L peptone, 3.0g/L beef extract powder, 5.0g/L sodium chloride and 15.0g/L agar powder, and performing high-pressure sterilization at 121 ℃ for 20min, and paving for later use.
(3) Mixing the bacillus subtilis YHX J spore concentrate prepared in the step (1) with the bacillus amyloliquefaciens YHX002J spore concentrate prepared in the step (2) according to the following weight ratio of 1:1.2 and diluted 3000 times followed by 1-2mg/L CaCl 2 After fully and uniformly mixing, the indoor environment microorganism cleanser is prepared, and the content of viable bacteria is 6 multiplied by 10 6 CFU/mL, the viable count ratio of the bacillus subtilis (Bacillus subtilis) YHX J to the bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YHX002J is 1:2, can be filled into small bottles (100 mL/bottle or 300 mL/bottle) according to actual demands.
Example 2 multiple skin irritation test of microbial cleaners
The skin irritation test of the microbial cleaners prepared according to the second section (2.3.3) of the "disinfection technical Specification" (2002 edition of the Ministry of health) is carried out as follows: 3 healthy adult female common New Zealand rabbits are selected, and the weight range is 2-3 kg; 24 hours before the test, the hairs on the two sides of the spine of the back of the New Zealand rabbit are shaved, and the skin cannot be damaged. The dehairing range was about 3cm by 3cm on the left and right. The next day 0.5mL of the test article (the cleaner prepared in example 1) was dropped onto 2 layers of gauze with an area of 2.5cm x 2.5cm and applied to one side of the dehaired intact skin, then covered with a layer of non-irritating plastic film and then fixed with non-irritating adhesive tape. The test substance is applied once a day for 14 days. The results were observed 24 hours after each application and scored, and the criteria are shown in tables 1 and 2 (sterilization specifications (2002 edition of Ministry of health)) tables 2-11 and 2-12. The control group was prepared in the same manner using 0.5mL of sterile water instead of the test substance. The experimental results are shown in table 3, and it can be seen that: the skin irritation index of the test object to New Zealand rabbits is 0, the skin irritation intensity is non-irritating, and the test object meets the standard requirements in the second part (2.3.13) of the disinfection technical Specification (2002 edition of Ministry of health) (in skin irritation test, if the test object has no irritation or only light irritation, the test object can pass).
TABLE 1 scoring criteria for skin irritation response
TABLE 2 skin irritation intensity grading
TABLE 3 skin irritation index of test subjects on New Zealand rabbits
EXAMPLE 3 acute inhalation toxicity test of microbial cleaners
Acute inhalation toxicity test was performed according to the second section (2.3.2) of the "sterilization technical Specification" (2002 edition of the Ministry of health), and the specific steps are as follows: taking 20 healthy Kunming mice, 10 male animals and 10 female animals, wherein the weight range is 18-22 g, adopting a dynamic contamination method, adopting a mechanical ventilation device, continuously and evenly feeding air containing a certain concentration of cleaning agent into a contamination cabinet, discharging equal amount of contamination gas, and maintaining relatively stable contamination concentration. One time of inhalation is infected for 2 hours. The spray method is adopted in the field, and the spray or ultrasonic atomizer is adopted to atomize the powder and then the atomized powder is input into a contamination cabinet.
The experiment selects one time for 2 hours to inhale 10000mg/m of the concentration of the microbial cleanser prepared in the example 1 3 The animals were observed for their toxic appearance and number of deaths and time to death after inhalation, and their body weights were measured at 0d, 7d, and 14d after exposure. Necropsy was performed on dead animals and on animals sacrificed at the expiration of the observation period, 14d, if any abnormality was found to be necessary for further pathological examination. According to the evaluation criteria: LC (liquid Crystal) device 50 2h is more than 10000mg/m 3 The patient is practically nontoxic; LC (liquid Crystal) device 50 2h is 1001mg/m 3 ～10000mg/m 3 The drug belongs to low toxicity; LC (liquid Crystal) device 50 2h is 101mg/m 3 ～1000mg/m 3 The patients are moderate in toxicity; LC (liquid Crystal) device 50 2h is 10mg/m 3 ～100mg/m 3 The patients are highly toxic; LC (liquid Crystal) device 50 2h is less than 10mg/m 3 The drug belongs to severe toxicity.
As shown in Table 4, the test results showed that the groups did not die or lose weight, and the test subjects had half-lethal LC in acute inhalation toxicity to Kunming mice 50 Greater than 10000mg/m 3 The acute toxicity dose is classified as practically nontoxic, and meets the standard requirements (LD 50 > 10000mg/m in acute inhalation toxicity test) in the second part (2.3.13) of the "Disinfection technical Specification (2002 edition of Ministry of health) 3 And is practically nontoxic and can pass through).
TABLE 4 acute inhalation toxicity test results
EXAMPLE 4 inhibition of indoor pathogenic microorganisms by microbial cleaners
The microbial cleaner prepared in example 1 was filled into 300mL atomizing bottles containing cotton cores, and the microbial cleaner in the atomizing bottle was released into the environment with a certain frequency using a pleasant breathing air cleaner (the machine has an atomizing function) with a spraying frequency of 1 time/h and a spraying amount of 0.13-0.15mL each time. Three families (old families, pet-raising families and children families) and four places (oral cavity clinics, medical centers, offices and hotel rooms) are respectively measured, the content of pathogenic microorganisms in surface microorganisms in an environment is detected after the environment is continuously used for 4 weeks, a swab wiping method is adopted for the microorganism sampling method to refer to national standards, and the content of the indoor pathogenic microorganisms is represented by the number of bacteria with hemolytic circles on blood plates due to the fact that the indoor common pathogenic microorganisms (including opportunistic pathogens) often comprise a plurality of or more types, and the indoor common pathogenic microorganisms such as staphylococcus aureus, streptococcus hemolyticus, acinetobacter and the like have hemolysis. The specific method comprises the following steps: after sampling, the test tube with the cotton swab after sampling is fully shaken or vortexed, and is diluted to 10 in gradient -5 According to the estimation of the sample pollution condition, 1mL of 3 sample homogenates with proper dilutions are selected in 2 sterile plates, and then 15-20mL of cooled blood agar medium is poured into the blood and uniformly mixed. After solidification, the plate was turned over, cultured at 36.+ -. 1 ℃ for 48 hours.+ -. 2 hours, and then the number of the lysogens was counted. The blood agar medium consisted of: 10.0g/L peptone, 3.0g/L beef extract powder, 5.0g/L sodium chloride, 50m/L defibrinated sheep blood, 0.1g/L natamycin and 15.0g/L agar powder.
As can be seen from fig. 1, after the microbial cleaner is used, the loading amount of pathogenic microorganisms characterized by hemolysis is obviously reduced in different places, and especially in pet care families and children families, the microbial cleaner has obvious inhibition effect on the pathogenic microorganisms.
Example 5 Effect of microbial cleaners on degradation of indoor allergens
The microbial cleaner prepared in example 1 was filled into 300mL atomizing bottles containing cotton cores, and the microbial cleaner in the atomizing bottle was released into the environment with a certain frequency using a pleasant breathing air cleaner (the machine has an atomizing function) with a spraying frequency of 1 time/h and a spraying amount of 0.13-0.15mL each time. Respectively adopting ELISA method to measure allergen content of a household study room and bedroom area of a pet before and after use, wherein the collected sample is house dust, the detection method is carried out by referring to a kit, the used kit is purchased from Indoor Biotechnologies, the house dust mite allergen detection kit Der f1 product number is EL-DP1, the dust mite allergen detection kit Der p1 product number is EL-DF1, the cat allergen detection kit Fel d1 product number is EL-FD1, the cockroach allergen Bla g1 detection kit product number is EL-BG1, and the allergen detection result is as follows:
TABLE 5 allergen content Change before and after application of microbial cleaners in study area
ND: the content was not detected, and was found to be below the detection limit.
TABLE 6 allergen content Change before and after use of microbial Environment scavenger in bedroom area
ND: the content was not detected, and was found to be below the detection limit.
EXAMPLE 6 inhibition of mites by microbial cleaners
The specific operation steps are as follows: three textile fabrics with the diameter of 80mm and three textile fabrics with the sterile water treatment are respectively placed in 6 metal plates after being treated by the microbial cleaner prepared in the example 1 (the treatment method is that the textile fabrics with the diameter of 80mm are immersed in the microbial cleaner for 10min and naturally drained by using sterile forceps), round filter paper with the diameter of 50mm is respectively placed in the center of the treated textile fabrics, mite baits are placed around the filter paper, then about 200 adult house dust mites are placed in the circumference of the filter paper, a metal plate cover is covered, and the edges are sealed: placing the 6 sealed metal plates in an incubator in a dark environment for cultivation, wherein the temperature in the incubator is 24 ℃, the relative humidity is 70%, respectively culturing for 24 hours, taking out test samples, observing under a microscope, calculating the number of visible mites on the upper layer of the woven cloth, and adding the live mite data of every three parallel samples to obtain the live mite number of the test sample group or the live mite number of the control group. Mite inhibition rate was calculated as follows: r= (A-B)/A multiplied by 100%, A is the number of live mites of a control group, B is the number of live mites of an experimental group, and the mite inhibition rate of the microbial cleaning agent prepared in the example 1 is 98.94% through calculation, so that the product has good dust mite inhibition activity.
EXAMPLE 7 Effect of microbial cleaners on formaldehyde removal
The formaldehyde removal effect of the microbial cleaner described in example 1 was measured by using a QB/T2761-2006 method for measuring the purification effect of an indoor air purification product, and the specific steps are as follows: with two 1.5m 3 The air test cabin (A is a blank cabin and B is a sample cabin) is used for testing, and the test conditions are carried out at normal temperature and normal pressure. The test cabin is pretreated before the experiment, in particular, the material of the test cabin body has no adsorptivity, the chemical reaction is inert (such as glass or stainless steel), the inner surface of the test cabin body has smooth, all the joints are sealed by using the material with air tightness (such as silicon rubber), and the air leakage rate of the test cabin is ensured to be less than 0.05m 3 And/h, the air in the test bin is nontoxic, harmless and odorless, and then a formaldehyde release source is prepared. Suspending untreated base paper (inert material) in a blank test cabin A, and suspending the base paper sprayed with a microbial cleaner (the dosage is 100 g) in a test cabin B; then the formaldehyde release source is respectively placed in the blank test cabin A and the sample test cabin B, and the cabin doors are immediately closed. And (3) starting fans of the blank test cabin A and the sample test cabin B, stirring for 1min, uniformly mixing air in the cabin with pollutants released by a release source, closing the fans, sampling air in the blank cabin, determining the concentration value of the pollutants in the air in the blank cabin as initial concentration, and sampling the concentration of formaldehyde in the two cabins after 24h, wherein the results are shown in the following table: it can be seen from table 7 that the cleaner has a high formaldehyde removal rate.
TABLE 7 Formaldehyde removal Effect
The above examples illustrate: (1) The indoor microbial cleaning agent has good inhibition effect on indoor pathogenic microorganisms in practical application, can effectively reduce the loading capacity of the indoor pathogenic microorganisms in different environments, and reduces the pathogenic risk; (2) The indoor microbial cleaning agent disclosed by the invention has good safety; (3) The indoor microbial cleanser provided by the invention has a good degradation effect on common indoor allergens (house dust mite allergens, wool allergens and cockroach allergens) in practical application, and effectively reduces allergy risks; (4) The indoor microbial cleaning agent can effectively inhibit the activity of dust mites, and is beneficial to reducing the harm of the indoor dust mites; (5) The indoor microbial cleaning agent disclosed by the invention can effectively remove harmful substances such as formaldehyde.
Claims (7)
1. A microbial cleaner, comprising a microbial cleaner having a preservation number of CCTCC NO: m2022266 Bacillus subtilisBacillus subtilis) YHX001J, and the preservation number is CCTCC NO: m2022265 Bacillus amyloliquefaciensBacillus amyloliquefaciens）YHX002J。
2. The microbial cleaner of claim 1, wherein bacillus subtilis @Bacillus subtilis) YHX001J and Bacillus amyloliquefaciensBacillus amyloliquefaciens) The ratio of the viable count of YHX002J is 1:2.
3. the microbial cleaner of claim 1, further comprising CaCl 2 。
4. Use of a microbial cleaner according to any one of claims 1 to 3 for inhibiting indoor pathogenic microorganisms, comprising staphylococcus aureus, streptococcus hemolyticus, acinetobacter.
5. Use of a microbial cleaner according to any one of claims 1 to 3 for degrading allergens, including dust mite allergens, house dust mite allergens, cat allergens, cockroach allergens.
6. Use of a microbial cleaner according to any one of claims 1 to 3 for inhibiting or killing dust mites.
7. Use of a microbial cleaner according to any one of claims 1 to 3 for formaldehyde removal.
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