CN116077600A - Mixture for eliminating infantile malnutrition and invigorating stomach - Google Patents
Mixture for eliminating infantile malnutrition and invigorating stomach Download PDFInfo
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Abstract
The invention discloses a mixture for resolving malnutrition and invigorating stomach, which comprises the following components in parts by weight: comprises 3-7 parts of edible herbal tea, 3-7 parts of dried orange peel, 8-12 parts of hawthorn, 8-12 parts of poria cocos, 13-17 parts of malt, 13-17 parts of rice sprout and 1-5 parts of fried chicken's gizzard-skin; the decoction has effects of resolving food stagnation, invigorating spleen and regulating stomach, and can be used for the adjuvant treatment of constipation or diarrhea accompanied with inappetence, abdominal distention and pain, and functional dyspepsia.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a mixture for resolving malnutrition and invigorating stomach.
Background
Constipation refers to a decrease in the number of stools, generally less than 3 times per week, with difficulty in defecation and dry stool. Constipation is a clinically common symptom, and is often persisted for a long time, so that the quality of life is influenced, the etiology is various, and intestinal diseases are the most common;
diarrhea refers to digestive dysfunction, which is mainly manifested by increased defecation amount, and can be classified into acute and chronic diarrhea according to severity;
wherein constipation or diarrhea is accompanied by inappetence, abdominal distention, functional dyspepsia and other symptoms, western medicines are mostly adopted for treatment, and the western medicines have more side effects, so that a traditional Chinese medicine mixture for treating the symptoms is necessary.
Disclosure of Invention
The invention aims to provide a mixture for resolving malnutrition and strengthening stomach, which solves the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a mixture for resolving malnutrition and invigorating stomach, which comprises the following components in parts by weight: comprises 3-7 parts of edible herbal tea, 3-7 parts of dried orange peel, 8-12 parts of hawthorn, 8-12 parts of poria cocos, 13-17 parts of malt, 13-17 parts of rice sprout and 1-5 parts of fried chicken's gizzard-skin.
The mixture for eliminating infantile malnutrition and invigorating stomach comprises the following components in parts by weight: comprises 5 parts of herbal tea, 5 parts of dried orange peel, 10 parts of hawthorn, 10 parts of poria cocos, 15 parts of malt, 15 parts of rice sprout and 3 parts of fried chicken's gizzard-skin.
Compared with the prior art, the invention has the beneficial technical effects that,
the decoction has effects of resolving food stagnation, invigorating spleen and regulating stomach, and can be used for the adjuvant treatment of constipation or diarrhea accompanied with inappetence, abdominal distention and pain, and functional dyspepsia.
Drawings
Fig. 1 effect of the mixture for resolving malnutrition and invigorating stomach on intestinal tract propulsion of constipation rats.
Figure 2 effect of the malnutrition and stomach strengthening mixture on dry weight of constipation rats.
FIG. 3 shows the effect of the mixture for treating constipation and invigorating stomach on the number of constipation rats.
Fig. 4 effect of the mixture for resolving malnutrition and invigorating stomach on the moisture content of constipation rat feces.
Figure 5 effect of the malnutrition and stomach strengthening mixture on serum nitric oxide levels in constipation rats.
FIG. 6 effect of the Xiaoqianjian mixture on serum motilin levels in constipation rats.
Fig. 7 effect of the malnutrition-eliminating and stomach-invigorating mixture on serum gastrin levels in constipation rats.
FIG. 8 effect of the Xiaoqianjian mixture on serum 5-hydroxytryptamine levels in constipation rats.
Figure 9 effect of the mixture for resolving malnutrition and invigorating stomach on constipation rat ileal alpha-glucosidase.
Figure 10 effect of the malnutrition and stomach strengthening mixture on constipation rat ileal cellulases.
FIG. 11 effect of the mixture for treating constipation and strengthening stomach on amylase in rats.
Fig. 12 effect of the malnutrition-eliminating and stomach-invigorating mixture on intestinal tract propulsion of rats.
Fig. 13 effect of the mixture for treating infantile malnutrition and invigorating stomach on the stool dilution rate, diarrhea index and intestinal tract propulsion rate of rats in 5 h.
Figure 14 effect of the malnutrition and stomach strengthening mixture on serum nitric oxide levels in diarrhea rats.
Fig. 15 effect of the malnutrition-promoting and stomach-invigorating mixture on serum gastrin levels in diarrhea rats.
FIG. 16 effect of the Xiaoqianjian mixture on serum motilin and 5-hydroxytryptamine levels in diarrhea rats.
FIG. 17 effect of the mixture for treating constipation, ileum cellulase, alpha-amylase, alpha-glucosidase.
Wherein (< 0.05, <0.01,) P.
Detailed Description
The invention will be further described with reference to the accompanying drawings and specific examples.
A mixture for resolving malnutrition and invigorating stomach, which comprises the following components in parts by weight: comprises 3-7 parts of edible herbal tea, 3-7 parts of dried orange peel, 8-12 parts of hawthorn, 8-12 parts of poria cocos, 13-17 parts of malt, 13-17 parts of rice sprout and 1-5 parts of fried chicken's gizzard-skin.
The mixture for eliminating infantile malnutrition and invigorating stomach comprises the following components in parts by weight: comprises 5 parts of herbal tea, 5 parts of dried orange peel, 10 parts of hawthorn, 10 parts of poria cocos, 15 parts of malt, 15 parts of rice sprout and 3 parts of fried chicken's gizzard-skin.
The herbal tea is a specific medicinal material of she nationality, is currently received in 2015 edition Zhejiang province traditional Chinese medicine processing Specification, has the effects of dispelling wind and relieving exterior syndrome, clearing heat and detoxicating, regulating qi and strengthening spleen, and eliminating diarrhea and relieving diarrhea, and clinically has better treatment effects on wind-heat exterior syndrome, spleen deficiency and food stagnation, diarrhea, epigastralgia, noisy and acid regurgitation; pericarpium Citri Tangerinae, fructus crataegi, fructus Hordei Germinatus, and fructus oryzae, and Poria have effects of regulating qi-flowing, removing food stagnation, promoting digestion, invigorating spleen, and calming heart. The special herbal medicine of she nationality is edible herbal tea, and is a special herbal medicine of the prescription and a monarch drug of the prescription.
Study on intestine-moistening and bowel-relaxing effects of the mixture for removing infantile malnutrition and invigorating stomach on rats:
1. experimental materials
1.1 animals
SPF-class wistar rats (190.+ -.20) g, females, purchased from Shanghai Laek laboratory animal Co., ltd., animal production pass number: SCXK 2017-0005. The plant is raised in SPF animal house of laboratory animal center of Zhejiang province, barrier system, artificial light, each day of light and darkness for 12h, the temperature is controlled at 20+ -5 deg.C, and the relative humidity is controlled at 50% -60%.
1.2 pharmaceutical products and reagents
Nitric Oxide (NO) test kit (lot A013-2-1), amylase (AMS) test kit (lot C016-1-1), cellulase (CL) test kit (lot A138-1-1), rat 5-hydroxytryptamine (5-HT) kit (lot H104-1-R), rat Gastrin (GAS) kit (lot H239-R), rat Motilin (MTL) kit (lot H182-R), rat alpha-glucosidase (alpha-GLU) kit (lot H419-1-R) were all purchased from Nanj to build the bioengineering institute. Loperamide hydrochloride capsules (national drug standard H10910085, lot No. LFJ 8684) were purchased from western ampelopsis, inc. Domperidone (dom) tablet (national standard H20123058, lot 2109151) was purchased from co-kernel pharmaceutical industries, inc.
1.3 instruments
Spectra Max190 full wavelength microplate reader (America Molecular Devices); MM400 freeze-mix ball mill (Retsch company, germany); sorvall ST 40R high capacity cryogenic centrifuge (Thermo company of America)
2. Experimental method
2.1 pharmaceutical formulation
Decocting decoction pieces with 8 times of water for 2 times, filtering each time for 2 hours, concentrating the filtrate to a proper amount, adding 2.0g of sodium benzoate and 40g of sucrose, concentrating to 1000ml of water, uniformly mixing, standing at 2-5 ℃ for 48 hours, taking supernatant, standing again for 24 hours, taking supernatant, filling and sealing, sterilizing at 110 ℃ for 30 minutes, then freezing the water in the liquid medicine in advance by adopting a vacuum freeze-drying method, and sublimating the frozen water in the liquid medicine under a vacuum sterile environment, thereby obtaining freeze-dried powder.
2.2 grouping of laboratory animals
The 20 wistar rats were randomly divided into four groups after one week of adaptive feeding: control group 5, model group 5, pan Duo rison group 5, malnutrition-eliminating and stomach-invigorating mixture group 5. The rats constipation model is built by the administration of 3mg/kg/d loperamide hydrochloride by intragastric administration of each group except the control group, and the control group is intragastric equivalent physiological saline. After 6 hours, rats with the mixture for curing infantile malnutrition and strengthening stomach are given 620mg/kg/d of lyophilized powder of the mixture for curing infantile malnutrition and strengthening stomach, and Pan Duo and the ketone groups are infused with 4.5mg/kg/d Pan Duoli ketone, and the control group and the model group are infused with normal saline with the same amount for 3 weeks.
2.3 animal body weight
During the course of the experiment, rat body weight was recorded weekly.
2.4 animal feces and moisture content determination
At the end of week 3 of the experiment, stool volume was recorded. After the drug is filled, the rat is placed in a metabolism cage, after 16 hours, animal feces are collected and weighed to be the wet weight W1 of the feces, then the animal feces are placed in a constant temperature drying oven at 50 ℃ for drying for 4 hours and weighed to be the dry weight W2 of the feces. The fecal moisture content was calculated as follows:
fecal moisture (%) = (W1-W2)/w1×100
Wherein W1 represents wet weight of feces, unit g; w2 represents fecal dry weight in g.
2.5 determination of intestinal Propulsion
After the last administration, the rats are fed with 2mL of 10% active carbon after being fed with stomach for 30min without water forbidden for 12 h. Starting timing after 10% of activated carbon is filled, anesthetizing after 20min, killing the rat after abdominal aorta blood taking, taking out the lower end of the stomach from the section of the abdomen to the whole intestinal tract of the pylorus, straightening, measuring the total length (L1) of the intestinal tract and the propelling distance (L2) of the ink in the intestinal tract in a tensionless state, calculating the percentage of the ink propelling distance to the total length of the intestinal tract, and carrying out statistical analysis. Intestinal tract propulsion (D) =l2/l1×100%.
2.6 serum Biochemical index determination
After anesthetizing rats with 3% pentobarbital, the abdominal aorta was bled, centrifuged at 3500r/min for 10min at 4 ℃, the supernatant serum was taken, and the motilin, gastrin, endothelin and nitric oxide levels in the serum were determined using an ELISA kit.
2.7 determination of ileal enzyme Activity
The group of rats was taken 1-2 cm (mass about 100 mg) of ileum 2cm away from the cecum, 900. Mu.L of physiological saline was added, homogenized, centrifuged at 3500r/min at 4℃for 10min, and the supernatants were assayed for cellulase, alpha-amylase, alpha-glucosidase in the ileum using ELISA kit.
2.8 data analysis
Mean ± standard deviation for all data
And (3) representing. T test and single factor analysis of variance are carried out by adopting GraphPad Prism 7.0 statistical software, and P is smaller than 0.05 to consider that the difference has statistical significance
3 results of experiments
3.1 rat weight changes
The data are shown in Table 1, and the control group, the Pan Duo risone group and the Xiaoanweike mixture group have no significant difference (P > 0.05) compared with the model;
TABLE 1 variation of rat body weight
3.2 effects of the mixture for treating infantile malnutrition and invigorating stomach on constipation and intestinal peristalsis
As can be seen from fig. 1, the intestinal tract propulsion rate of the model group is significantly reduced (P < 0.05) compared with the control group; compared with the model group, the ink propelling rates of the mixture group for promoting the digestion of the malnutrition and the domperidone group are obviously increased (P is less than 0.01), and the function of promoting the peristalsis of the mixture for promoting the digestion of the malnutrition is similar to that of the domperidone.
As can be seen from fig. 2, the fecal dry weight of the model group was significantly reduced (P < 0.01) compared to the control group; compared with the model group, the stool dry weight of the mixture group for promoting the digestion of the malnutrition and the domperidone group is obviously increased (P < 0.01), and the stool function of the mixture for promoting the digestion of the malnutrition is similar to that of the domperidone.
As can be seen from fig. 3, the fecal count of the model group was significantly reduced (P < 0.01) compared to the control group; compared with the model group, the feces number of the mixture group for promoting the digestion of the malnutrition and the domperidone group is obviously increased (P < 0.01), and the defecation function of the mixture for promoting the digestion of the malnutrition is similar to that of the domperidone.
As can be seen from fig. 4, the fecal moisture content of the model group was significantly reduced (P < 0.01) compared to the control group; compared with the model group, the fecal water content of the mixture group for promoting the digestion and the domperidone group is obviously increased (P < 0.01), and the fecal function of the mixture for promoting the digestion of the malnutrition is similar to that of the domperidone.
In conclusion, the mixture for promoting the digestion of the infantile malnutrition can increase gastrointestinal peristalsis, reduce the reabsorption of water by intestinal tracts, and prompt that the mixture for promoting the digestion of the infantile malnutrition has the effect of relieving constipation symptoms caused by loperamide hydrochloride.
3.2 Effect of the Ganjiao stomach-invigorating mixture on the serum motilin, 5-hydroxytryptamine, gastrin and nitric oxide levels in constipation rats
As shown in fig. 5, the serum nitric oxide levels in rats in the model group were significantly elevated (P < 0.05) compared to the control group; compared with the model group, the nitric oxide level in the serum of rats of the mixture group for eliminating infantile malnutrition and strengthening stomach and the domperidone group is obviously reduced (P is less than 0.05), and the mixture for eliminating infantile malnutrition and strengthening stomach is similar to domperidone in terms of regulating and controlling the nitric oxide level in the serum.
As shown in FIG. 6, the motilin level in the serum of rats in the model group was significantly reduced (P <0.05, 0.01) compared with the control group; compared with the model group, the serum motilin level of rats of the mixture group for promoting the digestion of the malnutrition and the domperidone group is obviously increased (P < 0.01), and the mixture for promoting the digestion of the malnutrition is similar to the domperidone in terms of regulating the serum motilin level.
As shown in fig. 7, the serum gastrin level was significantly reduced (P <0.05, 0.05) in the rats of the model group compared to the control group; compared with the model group, the serum gastrin level of the compound group for eliminating infantile malnutrition and strengthening stomach and the domperidone group rats is obviously increased (P <0.05,0.01), and the compound for eliminating infantile malnutrition and strengthening stomach is superior to domperidone in regulating and controlling the serum motilin level.
As shown in fig. 8, there was no significant change in 5-hydroxytryptamine levels in the serum of rats in the model group compared to the control group; compared with the model group, the levels of 5-hydroxytryptamine in the serum of rats in the malnutrition-eliminating and stomach-invigorating mixture group and the domperidone group have no significant change.
Finally, the mixture for eliminating infantile malnutrition and strengthening stomach plays a role in relieving constipation caused by loperamide hydrochloride by regulating nitric oxide water, motilin and gastrin in serum factors.
3.3 Effect of the mixture for treating infantile malnutrition and invigorating stomach on constipation rat ileum cellulase, alpha-amylase, alpha-glucosidase
As shown in fig. 9, the α -glucosidase levels were significantly elevated (P < 0.01) in the ileum of the model group rats compared to the control group; compared with the model group, the levels of alpha-glucosidase in the ileum of rats in the group of the mixture for promoting the digestion of the malnutrition and the domperidone are obviously reduced (P < 0.01), and the mixture for promoting the digestion of the malnutrition is similar to the domperidone in terms of regulating the levels of the alpha-glucosidase in the ileum.
As shown in fig. 10, the cellulase levels in the ileum of the model group rats were significantly elevated (P < 0.01) compared to the control group; compared with the model group, the cellulase level in the ileum of rats in the group of the mixture for promoting the digestion of the malnutrition and the domperidone is obviously reduced (P < 0.01), and the mixture for promoting the digestion of the malnutrition is similar to the domperidone in terms of regulating the cellulase level in the ileum.
As shown in FIG. 11, the amylase levels in the ileum of the rats in the model group were significantly elevated (P < 0.01) compared to the control group; compared with the model group, the amylase level in the ileum of rats in the group of the mixture for promoting the digestion of the malnutrition and the domperidone is obviously reduced (P < 0.01), and the mixture for promoting the digestion of the malnutrition is similar to the domperidone in terms of regulating the amylase level in the ileum.
Finally, the present invention provides a pharmaceutical composition for treating constipation caused by loperamide hydrochloride by restoring the normal activity of the enzymes in the ileum of rats.
The decoction has effects of resolving food stagnation, invigorating spleen and regulating stomach, and can be used for the adjuvant treatment of constipation accompanied with inappetence, abdominal distention and pain, and functional dyspepsia.
Study of diarrhea effects of the mixture for resolving malnutrition and invigorating stomach on rats:
1 Material
1.1 animals
SPF-class wistar rats (190.+ -.20) g, females, purchased from Shanghai Laek laboratory animal Co., ltd., animal production pass number: SCXK 2017-0005. The plant is raised in SPF animal house of laboratory animal center of Zhejiang province, barrier system, artificial light, each day of light and darkness for 12h, the temperature is controlled at 20+ -5 deg.C, and the relative humidity is controlled at 50% -60%.
1.2 pharmaceutical products and reagents
Nitric Oxide (NO) test kit (lot A013-2-1), amylase (AMS) test kit (lot C016-1-1), cellulase (CL) test kit (lot A138-1-1), rat 5-hydroxytryptamine (5-HT) kit (lot H104-1-R), rat Gastrin (GAS) kit (lot H239-R), rat Motilin (MTL) kit (lot H182-R), rat alpha-glucosidase (alpha-GLU) kit (lot H419-1-R) were all purchased from Nanj to build the bioengineering institute. Loperamide hydrochloride capsules (national drug standard H10910085, lot No. LFJ 8684) were purchased from western ampelopsis, inc.
2. Experimental method
2.1 pharmaceutical formulation
Decocting decoction pieces with 8 times of water for 2 times, filtering each time for 2 hours, concentrating the filtrate to a proper amount, adding 2.0g of sodium benzoate and 40g of sucrose, concentrating to 1000ml of water, uniformly mixing, standing at 2-5 ℃ for 48 hours, taking supernatant, standing again for 24 hours, taking supernatant, filling and sealing, sterilizing at 110 ℃ for 30 minutes, then freezing the water in the liquid medicine in advance by adopting a vacuum freeze-drying method, and sublimating the frozen water in the liquid medicine under a vacuum sterile environment, thereby obtaining freeze-dried powder.
50g of senna leaf is taken, 500mL of water is added, boiling is carried out for about 10mi, filtering is carried out, and the filtrate is decompressed and concentrated to prepare the liquid medicine with the crude drug content of 0.4 g/mL. 6mg of loperamide hydrochloride is taken and dissolved in 40mL of physiological saline to prepare a liquid medicine with the concentration of 0.15 mg/mL. 2.48g of the lyophilized powder of the mixture for resolving malnutrition and strengthening stomach is dissolved in 40mL of physiological saline to prepare a liquid medicine with the concentration of 62 mg/mL. The liquid medicine is stored in a refrigerator at 4 ℃ and is heated to 25 ℃ in water bath when in use.
2.2 grouping of laboratory animals
After 20 wistar rats were acclimatized for one week, the rats were randomly divided into four groups: control group 5, model group 5, loperamide hydrochloride group 5, and malnutrition eliminating group 5. The diarrhea model of rats is established by the administration of 4g/kg/d senna leaf solution by intragastric administration of each group except the control group, and the control group is intragastric equivalent physiological saline. After 6 hours, the rats in the malnutrition-eliminating group are given with 620mg/kg/d of the mixture solution for promoting the digestion of the malnutrition, the loperamide hydrochloride group is infused with 1.5mg/kg/d of the loperamide hydrochloride solution, and the control group and the model group are infused with the same amount of physiological saline, and the administration volume is 10mL/kg; the administration was for 3 weeks.
2.3 animal body weight
During the course of the experiment, rat body weight was recorded weekly
2.3 cases of animal diarrhea index
At the end of experiment 3, the diarrhea index was recorded. Rats were placed in a metabolic cage, clean filter paper was placed under the metabolic cage, and after 5 hours, filter paper was collected for each rat faeces after dosing, and the diarrhea index for each group was calculated according to the following formula.
Diarrhea index = runny bowel rate x average runny bowel grade.
Average rare grade = all rare grades/rare times.
Rare rate = number of animals diarrhea/total number of animals in the group x 100%.
Wherein the loose stool rate is the ratio of the number of loose stools discharged by each animal to the total stool number. The loose stool grade indicates the degree of loose stool of each animal, and is classified into 4 grades according to the diameter of the loose stool polluted filter paper: less than 1cm is l grade, 1-1.9 cm is 2 grade, 2-3 cm is 3 grade, and more than 3cm is 4 grade. The diarrhea latency time refers to the time interval during which diarrhea first occurs after the senna is intragastric.
2.4 determination of intestinal Propulsion
After the last administration, the rats are fed with 2mL of 10% active carbon after being fed with stomach for 30min without water forbidden for 12 h. Starting timing after 10% of activated carbon is filled, anesthetizing after 20min, killing the rat after abdominal aorta blood taking, taking out the lower end of the stomach from the section of the abdomen to the whole intestinal tract of the pylorus, straightening, measuring the total length (L1) of the intestinal tract and the propelling distance (L2) of the ink in the intestinal tract in a tensionless state, calculating the percentage of the ink propelling distance to the total length of the intestinal tract, and carrying out statistical analysis. Intestinal tract propulsion (D) =l2/l1×100%.
2.5 serum Biochemical index determination
After anesthetizing rats with 3% pentobarbital, the abdominal aorta was bled, centrifuged at 3500r/min for 10min at 4 ℃, the supernatant serum was taken, and the motilin, gastrin, endothelin and nitric oxide levels in the serum were determined using an ELISA kit.
2.6 determination of ileal enzyme Activity
The group of rats was taken 1-2 cm (mass about 100 mg) of ileum 2cm away from the cecum, 900. Mu.L of physiological saline was added, homogenized, centrifuged at 3500r/min at 4℃for 10min, and the supernatants were assayed for cellulase, alpha-amylase, alpha-glucosidase in the ileum using ELISA kit.
2.7 data analysis
Mean ± standard deviation for all data
And (3) representing. T test and single factor analysis of variance are carried out by adopting GraphPad Prism 7.0 statistical software, and P is smaller than 0.05 to consider that the difference has statistical significance
3 results of experiments
3.1 rat weight changes
The data are shown in Table 2, and there was no significant difference (P > 0.05) between the control group, loperamide hydrochloride group and the Xiaoanweike mixture group compared to the model.
TABLE 2 variation of rat body weight
3.2 Effect of the mixture for resolving infantile malnutrition and invigorating stomach on rat Dilute-stool Rate, diarrhea index and intestinal Propulsion Rate
As can be seen from fig. 12, the intestinal tract propulsion rate of the activated carbon of the model group is significantly improved (P < 0.01) compared with the control group; the active carbon intestinal canal propulsion rate is obviously reduced (P < 0.01) after the stomach is infused, the stomach-strengthening and digestion-promoting mixture and the loperamide hydrochloride are administrated, wherein the loperamide hydrochloride is superior to the stomach-strengthening and digestion-promoting mixture in terms of weakening the active carbon intestinal canal propulsion rate.
As can be seen from fig. 13, the rate of loose stool and diarrhea index of the rats were significantly increased (P < 0.01) in the model group 5h compared to the control group; compared with the model group, the bowel movement rate of rats in the group of the mixture for eliminating the infantile malnutrition and strengthening the stomach and the loperamide hydrochloride group in 5 hours is obviously reduced (P <0.05,0.01), wherein the mixture for eliminating the infantile malnutrition and strengthening the stomach is weaker than the loperamide hydrochloride in the aspect of reducing the bowel movement index. The mixture for eliminating the infantile malnutrition and strengthening the stomach can weaken gastrointestinal peristalsis, promote the reabsorption of water by intestinal tracts, and prompt that the mixture for eliminating the infantile malnutrition and strengthening the stomach has the effect of relieving diarrhea symptoms caused by senna leaves.
3.3 Effect of the Ganjiao stomach-invigorating mixture on the serum motilin, 5-hydroxytryptamine, gastrin and nitric oxide levels in diarrhea rats
As shown in fig. 14, the serum nitric oxide levels in rats in the model group were significantly reduced (P < 0.05) compared to the control group; compared with the model group, the nitric oxide level in the serum of rats is obviously increased (P is less than 0.05) in the mixture group for eliminating the infantile malnutrition and strengthening the stomach and the loperamide hydrochloride group, and the mixture for eliminating the infantile malnutrition and strengthening the stomach is similar to the loperamide hydrochloride in terms of regulating and controlling the nitric oxide level in the serum.
As shown in fig. 15, the serum gastrin levels were significantly elevated (P < 0.05) in the rats of the model group compared to the control group; compared with the model group, after the stomach-filling and the stomach-strengthening mixture for curing infantile malnutrition and loperamide hydrochloride are administrated, the serum gastrin level of rats is obviously reduced (P <0.05,0.01), wherein the stomach-strengthening mixture for curing infantile malnutrition is slightly weaker than the loperamide hydrochloride in terms of reducing the serum gastrin level.
As shown in fig. 16, the motilin and 5-hydroxytryptamine in the serum of rats in the model group were not significantly changed (P > 0.05) compared to the control group; compared with the model group, the serum motilin and 5-hydroxytryptamine levels in the serum of rats of the malnutrition-eliminating and stomach-invigorating mixture group and the loperamide hydrochloride group have no significant change (P > 0.05). The mixture for resolving malnutrition and invigorating stomach can relieve diarrhea caused by senna by adjusting the level of nitric oxide and gastrin in serum factors.
3.4 Effect of the mixture for treating infantile malnutrition and invigorating stomach on diarrhea Crohn's cellulase, alpha-amylase, alpha-glucosidase
As can be seen from fig. 17, the cellulase, α -amylase, α -glucosidase activity was not significantly changed (P > 0.05) in the model group compared to the control group. Compared with the model group, the mixture group for resolving malnutrition and invigorating stomach and the loperamide hydrochloride group have no significant change (P > 0.05) in cellulase, alpha-amylase and alpha-glucosidase. The present inventors have suggested that the malnutrition-eliminating and stomach-invigorating mixture does not alleviate diarrhea caused by senna by modulating the enzymatic activity in the ileum.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention, but rather to enable any modification, equivalent replacement, improvement or the like to be included within the spirit and principles of the invention.
Claims (2)
1. The mixture for eliminating the infantile malnutrition and strengthening the stomach is characterized by comprising the following components in parts by weight: comprises 3-7 parts of edible herbal tea, 3-7 parts of dried orange peel, 8-12 parts of hawthorn, 8-12 parts of poria cocos, 13-17 parts of malt, 13-17 parts of rice sprout and 1-5 parts of fried chicken's gizzard-skin.
2. The mixture for resolving and invigorating stomach as claimed in claim 1, wherein the mixture for resolving and invigorating stomach comprises: comprises 5 parts of herbal tea, 5 parts of dried orange peel, 10 parts of hawthorn, 10 parts of poria cocos, 15 parts of malt, 15 parts of rice sprout and 3 parts of fried chicken's gizzard-skin.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446493A (en) * | 2013-08-01 | 2013-12-18 | 雷后兴 | She medicine for treating infantile malnutrition and using method of She medicine |
| CN104127761A (en) * | 2014-07-24 | 2014-11-05 | 山东宏济堂制药集团有限公司 | Children's soft preparation for strengthening stomach and promoting digestion and preparation method thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446493A (en) * | 2013-08-01 | 2013-12-18 | 雷后兴 | She medicine for treating infantile malnutrition and using method of She medicine |
| CN104127761A (en) * | 2014-07-24 | 2014-11-05 | 山东宏济堂制药集团有限公司 | Children's soft preparation for strengthening stomach and promoting digestion and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 张晓芹;刘忠达;王慧玉;: "基于文献综述对畲药食凉茶研究趋势的探讨", 中医药管理杂志, no. 15, pages 5 - 7 * |
| 袁慧强;张晓芹;雷后兴;: "畲药食凉茶组方治疗小儿疳证30例疗效观察", 浙江中医杂志, no. 02, pages 1 - 3 * |
| 雷后兴;: "畲族民间验方消积汤治疗小儿厌食症196例疗效观察", 中国民族医药杂志, no. 03, pages 15 * |
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