CN116076499A - Application of 3-methyl-1-butanol in preparing Tarsoprolus gemini She Jiaci insect attractant - Google Patents
Application of 3-methyl-1-butanol in preparing Tarsoprolus gemini She Jiaci insect attractant Download PDFInfo
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- CN116076499A CN116076499A CN202310194732.8A CN202310194732A CN116076499A CN 116076499 A CN116076499 A CN 116076499A CN 202310194732 A CN202310194732 A CN 202310194732A CN 116076499 A CN116076499 A CN 116076499A
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- PHTQWCKDNZKARW-UHFFFAOYSA-N isoamylol Chemical compound CC(C)CCO PHTQWCKDNZKARW-UHFFFAOYSA-N 0.000 title claims abstract description 58
- 239000002418 insect attractant Substances 0.000 title claims abstract description 10
- 239000005667 attractant Substances 0.000 claims abstract description 16
- 230000031902 chemoattractant activity Effects 0.000 claims abstract description 16
- 241000238631 Hexapoda Species 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 5
- 229940057995 liquid paraffin Drugs 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 241000027433 Apriona Species 0.000 claims description 2
- 241000220324 Pyrus Species 0.000 claims 1
- 239000004480 active ingredient Substances 0.000 claims 1
- 235000021017 pears Nutrition 0.000 claims 1
- 238000007493 shaping process Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 206010025421 Macule Diseases 0.000 abstract description 2
- 239000000575 pesticide Substances 0.000 abstract description 2
- 239000000447 pesticide residue Substances 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 description 13
- 238000012360 testing method Methods 0.000 description 11
- 230000004044 response Effects 0.000 description 9
- 239000000523 sample Substances 0.000 description 6
- 239000013043 chemical agent Substances 0.000 description 4
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 4
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 4
- 241001124134 Chrysomelidae Species 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 3
- 241000011500 Phyllostachys praecox Species 0.000 description 3
- 235000008545 Phyllostachys praecox Nutrition 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 239000003039 volatile agent Substances 0.000 description 3
- 241000254173 Coleoptera Species 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 241000489975 Diabrotica Species 0.000 description 1
- 235000011511 Diospyros Nutrition 0.000 description 1
- 241000723267 Diospyros Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000223924 Eimeria Species 0.000 description 1
- 244000119298 Emblica officinalis Species 0.000 description 1
- 235000015489 Emblica officinalis Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000894800 Galerucinae Species 0.000 description 1
- 241000219146 Gossypium Species 0.000 description 1
- 241000254158 Lampyridae Species 0.000 description 1
- 241001142480 Monolepta Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an application of 3-methyl-1-butanol in preparing a Tarsonius bifidus She Jiaci insect attractant, belonging to the technical field of biological control of agricultural pests. The 3-methyl-1-butanol provided by the invention has remarkable attracting activity on the Metropriopsis variabilis, and the 3-methyl-1-butanol is used as the attractant component of the Metropriopsis variabilis She Jiaci, so that the invention has the characteristics of simple and convenient operation, high attracting efficiency, high feasibility in field use, strong practicability and the like. The popularization and application of the invention can obviously reduce the application amount of chemical pesticides in preventing and treating the long-tarsonemus ohba, thereby reducing the pesticide residue and other problems. The invention has important application value in biological control of the double-macula tarsonensis and has wide economic, social and ecological values.
Description
Technical Field
The invention belongs to the technical field of biological control of agricultural pests, and particularly relates to application of 3-methyl-1-butanol in preparation of a Tarsonia dubia She Jiaci insect attractant.
Background
Tarsoprolease amphibian leaf beetles Monolepta signata belong to the order Coleoptera, chrysomelidae, the subfamily Fluodenae Galerucinae, the alias Diospyros. The phyllanthus emblica is worldwide distributed in multiple countries in the eastern and southeast asia. The double-spot long-tarsal firefly leaf beetle is a multi-feeding insect, can feed on corn, cotton, sorghum, beans, cruciferous vegetables and other crops, mainly feeds on leaves, flowers and clusters of host plants by adults, has the characteristics of long hazard period, high insect population density, strong fertility, rapid pest diffusion and the like, and seriously influences the growth of plants.
Recent researches find that the diabrotica spp has a tendency to aggravate harmful effects on crops such as cotton and corn, and has become a main pest on corn and cotton in partial areas. At present, the main means for preventing and treating the phyllostachys praecox is still to use chemical agents, but the chemical agents are not easy to degrade, the environment is easy to be damaged after long-term use, and the phyllostachys praecox has strong adaptability, and the use of the chemical agents can cause the phyllostachys praecox to generate drug resistance, so that the preventing and treating effect is also unsatisfactory. Therefore, in order to reduce the use of chemical agents, it is imperative to find a green, safe and efficient control strategy.
Chinese patent No. 112931503A discloses an attractant for adult beetles of Amriopsis cumingii, however, the attractant has more components, high preparation requirement and complicated practical application.
Disclosure of Invention
The invention aims to provide an application of 3-methyl-1-butanol in preparing a Tarsoprolus biggest She Jiaci insect attractant. The attractant has only one effective component, is simple to prepare, and has obvious attracting activity on the Metropriopsis variabilis.
In order to achieve the above purpose, the present invention provides the following technical solutions:
application of 3-methyl-1-butanol in preparing Tarsoprolus biggest She Jiaci insect attractant.
Preferably, the attractant consists of 3-methyl-1-butanol and a volatile solvent, and the effective component is a single component of 3-methyl-1-butanol.
Preferably, the concentration of 3-methyl-1-butanol in the attractant is 1 to 10000nL/mL, more preferably 10 μL/mL.
Preferably, the volatile solvent is liquid paraffin.
Preferably, the method for trapping or killing the Metropriopsis variabilis comprises the following steps: the carrier loaded with the attractant containing 3-methyl-1-butanol is placed in a field trapping device, and a trap is arranged to trap or kill the Apriona geminalis.
The beneficial technical effects of the invention are as follows:
the invention provides an application of 3-methyl-1-butanol in preparing a Tarsoprolus gemini She Jiaci insect attractant, wherein the 3-methyl-1-butanol has remarkable attracting activity on the Tarsoprolus gemini Eimeria, and the 3-methyl-1-butanol is used as an attractant component of the Tarsoprolus gemini She Jiaci insect attractant, and has the characteristics of simple and convenient operation, high attracting efficiency, high feasibility in field use, strong practicability and the like. The popularization and application of the invention can obviously reduce the application amount of chemical pesticides in preventing and treating the long-tarsonemus ohba, thereby reducing the pesticide residue and other problems. The invention has important application value in biological control of the double-macula tarsonensis and has wide economic, social and ecological values.
Drawings
FIG. 1 is a statistical chart showing the results of the behavioral response of Tarsoprolus gemini She Jiaci insects to body surface volatiles in example 2.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention. It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
Example 1
The antennal potential (EAG) response of each test reporter compound in table 1 to the amoebola bifidus is determined:
table 1 test information compounds
(1) Selecting an actively and robust adult two-spotted long-tarsonemus ohba, cutting off the antenna from the base by an ophthalmic surgical knife and cutting off the tail end by 1mm, putting the antenna on an EAG probe coated with Spectra 360 conductive adhesive, and then debugging the instrument to enable the baseline of the instrument to be stable.
(2) 10. Mu.L of the formulation (see Table 2) was added dropwise to a 5mm by 4cm strip of filter paper, and the strip of filter paper was placed in a 1mL gun, and the volatiles were volatilized for 30 seconds by plugging both ends of the gun.
(3) The pulse stimulation gas flow rate was set at 600mL/min and the continuous humidified gas flow rate was 500mL/min. Each stimulus was 1s, with a 2 stimulus interval of 30s.
(4) After the EAG signal is stable, the response value of the antenna potential is recorded. All the compounds to be tested are tested by taking liquid paraffin as a control and normal hexane as a reference, and each test sample is repeated 6 times, and the measurement sequence of each repetition is as follows: control, reference, volatiles, control, reference. Each volatile unit was set up for 6 replicates.
The relative value of the antenna potential response (Sr) of the stimulus sample is calculated as follows:
in the formula, sc is the response value of the antenna potential of the stimulus sample; CKm is the average value of the response values of the control to the antenna potential before and after the stimulation of the sample; rm is the average of the standard compound antenna potentials before and after the stimulus sample antenna potential.
The results of the calculation of the relative values of the antenna potential response of the stimulated samples are shown in Table 2. Table 2 shows that the female Amaroot maculata had stronger electrophysiological reactivity to 3-methyl-1-butanol than the test compound.
TABLE 2 EAG relative response value of Tarsoprolus gemini She Jiaci insects to the test information compound (liquid Paraffin solvent)
Example 2
The attraction of Mesorus alternifolius on Mesorus alternifolius was determined by selecting a single-component message compound solution of example 1 at a concentration of 10. Mu.L/mL:
the experimental method comprises the following steps: based on the antenna electrophysiological reaction result, 10 mu L of volatile to be detected with the concentration of 10 mu L/mL is selected and dripped on filter paper, and paraffin with the concentration of 10 mu L is used as a contrast, and the volatile to be detected is quickly placed in an odor source bottle. The atmosphere sampling instrument is regulated to make the air flows of the two branches respectively 500mL/min. 1 head of strong double-stipitis longus formazan adults hungry for 12 hours are placed at the tail end of a main pipe during testing, when the two heads of the strong double-stipitis longus formazan adults cross 1/2 of a main pipe, timing is started, the selectivity of the two heads of the two-stipitis longus formazan adults is observed, and when a test worm crosses 1/2 of a test arm and stays for 5 seconds, the two heads of the two-stipitis longus formazan adults are marked as a reaction; if the reaction time is 5min, the reaction is marked as no reaction. The leaf beetles are tested only once, and the two arms are interchanged every 5 heads to eliminate the influence of residual smell among different treatments. Each of the information compound solutions was tested for 20 heads of Metropriopsis variabilis, repeated 4 times, and 80 heads total.
The reaction rate, the selective reaction rate and the selection coefficient of each information compound on the two-spotted tarsonella are calculated as follows:
a selection coefficient greater than 0 indicates that the tested insect has a tendency to the test compound, a greater value indicates a stronger tendency, and a maximum value of the selection coefficient is 1; if the selection coefficient is less than 0, this indicates that the insect has repellency to the test compound.
The results of the statistical analysis of example 2 are shown in FIG. 1, and the selective response rate of Tarsoprolease She Jiaci insects to the reporter compound at each concentration of 10. Mu.L/mL is shown in FIG. 1. The selective response rate of the Amaromyces biceps She Jiaci to the 3-methyl-1-butanol is obviously higher than that of other compounds, which proves that the 3-methyl-1-butanol has obvious attraction effect on Amaromyces biceps.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (6)
- Application of 3-methyl-1-butanol in preparing Tarsoprolus biggest She Jiaci insect attractant.
- 2. The use of 3-methyl-1-butanol according to claim 1 for the preparation of a two-spotted-brown pearsoid She Jiaci insect attractant, wherein said attractant consists of 3-methyl-1-butanol and a volatile solvent, and the active ingredient is a single component of 3-methyl-1-butanol.
- 3. The use of 3-methyl-1-butanol according to claim 2 for the preparation of an attractant for the tarsonella amphisarca She Jiaci, wherein the concentration of 3-methyl-1-butanol in the attractant is 1-10000 nL/mL.
- 4. Use of 3-methyl-1-butanol according to claim 3 for the preparation of a tar-shaping attractant for the two-spotted pears She Jiaci insects, wherein the concentration of 3-methyl-1-butanol in said attractant is 10 μl/mL.
- 5. The use of 3-methyl-1-butanol according to claim 2 for the preparation of a attractant for the tarsonella amphisarca She Jiaci insects, wherein said volatile solvent is liquid paraffin.
- 6. The use of 3-methyl-1-butanol in the preparation of an attractant for the amopalus biggest She Jiaci insects according to claim 1, wherein the method of trapping or killing the amopalus biggest comprises: the carrier loaded with the attractant containing 3-methyl-1-butanol is placed in a field trapping device, and a trap is arranged to trap or kill the Apriona geminalis.
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| CN202310194732.8A CN116076499B (en) | 2023-03-02 | 2023-03-02 | Application of 3-methyl-1-butanol in preparing Tarsoprolus gemini She Jiaci insect attractant |
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| CN202310194732.8A CN116076499B (en) | 2023-03-02 | 2023-03-02 | Application of 3-methyl-1-butanol in preparing Tarsoprolus gemini She Jiaci insect attractant |
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| CN116076499B CN116076499B (en) | 2024-03-22 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190653B1 (en) * | 1998-09-18 | 2001-02-20 | The United States Of America As Represented By The Secretary Of Agriculture | Chemical attractants for moths |
| US20120245126A1 (en) * | 2009-12-09 | 2012-09-27 | East China University Of Science And Technology | Divalent and oxabridged heterocyclic neonicotinoid compounds and preparation methods thereof |
| WO2014144685A2 (en) * | 2013-03-15 | 2014-09-18 | The Regents Of The University Of California | Methods for identifying arthropod repellents and attractants, and compounds and compositions identified by such methods |
| CN112931503A (en) * | 2021-02-07 | 2021-06-11 | 石河子大学 | Adult athyrium bicolor attractant |
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- 2023-03-02 CN CN202310194732.8A patent/CN116076499B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190653B1 (en) * | 1998-09-18 | 2001-02-20 | The United States Of America As Represented By The Secretary Of Agriculture | Chemical attractants for moths |
| US20120245126A1 (en) * | 2009-12-09 | 2012-09-27 | East China University Of Science And Technology | Divalent and oxabridged heterocyclic neonicotinoid compounds and preparation methods thereof |
| WO2014144685A2 (en) * | 2013-03-15 | 2014-09-18 | The Regents Of The University Of California | Methods for identifying arthropod repellents and attractants, and compounds and compositions identified by such methods |
| CN112931503A (en) * | 2021-02-07 | 2021-06-11 | 石河子大学 | Adult athyrium bicolor attractant |
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