CN116008438B - 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 - Google Patents
一种用于评价婴幼儿精细动作发育状况的标志物组及应用 Download PDFInfo
- Publication number
- CN116008438B CN116008438B CN202310119104.3A CN202310119104A CN116008438B CN 116008438 B CN116008438 B CN 116008438B CN 202310119104 A CN202310119104 A CN 202310119104A CN 116008438 B CN116008438 B CN 116008438B
- Authority
- CN
- China
- Prior art keywords
- sample
- infants
- marker
- temperature
- development condition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003550 marker Substances 0.000 title claims abstract description 26
- 238000011161 development Methods 0.000 title claims abstract description 18
- 230000009471 action Effects 0.000 title claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 25
- 235000020256 human milk Nutrition 0.000 claims abstract description 24
- 210000004251 human milk Anatomy 0.000 claims abstract description 24
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 13
- 229930195729 fatty acid Natural products 0.000 claims abstract description 13
- 239000000194 fatty acid Substances 0.000 claims abstract description 13
- 150000004665 fatty acids Chemical class 0.000 claims abstract description 13
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- FUKUFMFMCZIRNT-UHFFFAOYSA-N hydron;methanol;chloride Chemical compound Cl.OC FUKUFMFMCZIRNT-UHFFFAOYSA-N 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 3
- 239000012159 carrier gas Substances 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 230000032050 esterification Effects 0.000 claims description 3
- 238000005886 esterification reaction Methods 0.000 claims description 3
- 238000004817 gas chromatography Methods 0.000 claims description 3
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- XSTXAVWGXDQKEL-UHFFFAOYSA-N Trichloroethylene Chemical compound ClC=C(Cl)Cl XSTXAVWGXDQKEL-UHFFFAOYSA-N 0.000 claims description 2
- 238000004364 calculation method Methods 0.000 claims description 2
- 238000001819 mass spectrum Methods 0.000 claims description 2
- 238000007781 pre-processing Methods 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims 1
- 235000016709 nutrition Nutrition 0.000 abstract description 11
- 230000035764 nutrition Effects 0.000 abstract description 9
- 238000011156 evaluation Methods 0.000 abstract description 6
- 235000005911 diet Nutrition 0.000 abstract description 4
- 230000037213 diet Effects 0.000 abstract description 4
- 230000033001 locomotion Effects 0.000 abstract description 4
- 235000013365 dairy product Nutrition 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 description 11
- 235000013336 milk Nutrition 0.000 description 10
- 239000008267 milk Substances 0.000 description 10
- 210000004080 milk Anatomy 0.000 description 10
- 238000001514 detection method Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 235000019387 fatty acid methyl ester Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 210000000481 breast Anatomy 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000013499 data model Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000006694 eating habits Nutrition 0.000 description 2
- 235000019197 fats Nutrition 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 235000003441 saturated fatty acids Nutrition 0.000 description 2
- 150000004671 saturated fatty acids Chemical class 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 1
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 1
- 235000019528 24-hour dietary recall (24HR) Nutrition 0.000 description 1
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 1
- 208000032170 Congenital Abnormalities Diseases 0.000 description 1
- 206010010356 Congenital anomaly Diseases 0.000 description 1
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 1
- 239000005642 Oleic acid Substances 0.000 description 1
- 235000021314 Palmitic acid Nutrition 0.000 description 1
- 208000032023 Signs and Symptoms Diseases 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 230000007698 birth defect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 235000021196 dietary intervention Nutrition 0.000 description 1
- 235000008242 dietary patterns Nutrition 0.000 description 1
- MBMBGCFOFBJSGT-KUBAVDMBSA-N docosahexaenoic acid Natural products CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000004146 energy storage Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000008821 health effect Effects 0.000 description 1
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 1
- 150000003903 lactic acid esters Chemical class 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229940029985 mineral supplement Drugs 0.000 description 1
- 235000020786 mineral supplement Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000021281 monounsaturated fatty acids Nutrition 0.000 description 1
- 230000008111 motor development Effects 0.000 description 1
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 description 1
- 235000021048 nutrient requirements Nutrition 0.000 description 1
- 235000003715 nutritional status Nutrition 0.000 description 1
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 235000019195 vitamin supplement Nutrition 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P90/00—Enabling technologies with a potential contribution to greenhouse gas [GHG] emissions mitigation
- Y02P90/30—Computing systems specially adapted for manufacturing
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
本发明提供了一种用于评价婴幼儿精细动作发育状况的标志物组，其特征在于，所述标志物组由以下脂肪酸组成：C12，C4，C20，C20:3n6，C15:1，C20:4n6，C14，C6，C18：3n6，C22:1n9：C16:1，C22:6n3，C8，C24:0，C16，C21，C18:1n9c。本发明还提供了一种采用上述的标志物组评价婴幼儿精细动作发育状况的方法。本发明通过提取检测婴幼儿摄食的母乳或乳制品样品中特定的脂肪酸的含量，以实现对于该婴幼儿的精细动作发育状况的评价，并可根据评价结果推测该婴幼儿在同龄人中的发育水平，以便及时的对母体给出膳食和营养学的相关建议。该方法成本低廉，简便有效，适用于大规模的评价工作。
Description
技术领域
本发明涉及检测与营养学评价领域，特别是一种用于评价婴幼儿精细动作发育状况的标志物组及应用。
背景技术
脂肪是维持生命不可缺少的营养物质和基础代谢产物，它是机体内主要的能量储存形式。研究表明，脂肪的营养价值由脂肪酸决定。母乳是婴儿的主要食物。母乳中所含有的各种营养元素其种类、含量以及配比都是宝宝需要的“黄金标准”。母乳中脂肪酸的种类很多，中链及中长链饱和脂肪酸；长链饱和脂肪酸（如棕榈酸）；单不饱和脂肪酸（如油酸）；多不饱和脂肪酸（如亚油酸，ARA,DHA，亚麻酸）。
生命早期1000天指从母亲怀孕到幼儿2周岁,其营养状态影响一生的健康,成年期疾病的预防应从生命早期营养开始。成为当前国际上食品与医学领域的研究热点.越来越多的证据表明,以大数据为基础的营养素参考摄入量推荐值有助于科学地制定营养政策及婴幼儿喂养标准。母乳是婴幼儿的最佳食品,世界卫生组织推荐6月内婴儿纯母乳喂养,母乳的营养成分及含量动态变化是推测该阶段婴儿营养素需要的基础,但不同人种、不同膳食模式乳母的母乳成分差别很大。出生队列研究则主要探讨营养状况、生活方式、饮食习惯等暴露因素与不良妊娠结局及出生缺陷的关系、生命早期暴露对终身健康影响的优选方法。
因鉴于此，特提出此发明。
发明内容
本发明的目的在于，提供一种标志物组，通过检测样品中该标注物组中各标志物的含量，即可推算得到该样品喂养的婴幼儿的精细动作发育状况，以便进行及时的营养学建议和采取营养学干预的措施。
为了实现上述目的，本发明提供了一种用于评价婴幼儿精细动作发育状况的标志物组，所述标志物组由以下脂肪酸组成：C12，C4，C20，C20:3n6，C15:1，C20:4n6，C14，C6，C18：3n6，C22:1n9：C16:1，C22:6n3，C8，C24:0，C16，C21，C18:1n9c。
另一方面，本发明还提供了一种上述的标志物组评价婴幼儿精细动作发育状况的方法，按下述步骤依次进行：
（1）收集样品，并预处理，制得待测样品；
（2）采用气相色谱-质谱联用法测定待测样品中各标志物组标志物的含量；
（3）根据测定的各标志物含量计算该样品的得分Yb；
（4）根据得分Yb评价婴幼儿精细动作发育状况。
优选或可选地，步骤（1）中，样品的预处理方法为采用盐酸-甲醇法对样品进行甲酯化预处理。
优选或可选地，所述盐酸-甲醇法的具体工艺为取200μL样品，加入0.5mol/L的盐酸-甲醇溶液5mL，加入2mL正己烷，2mL甲醇，振荡混匀，在80℃下恒温水浴振荡2h，然后用流动水冷却至室温，再加入2mL去离子水，振荡混匀，于5000rpm下冷冻离心5min，取1mL上层清液于进样瓶，即得待测样品。
优选或可选地，步骤（2）中，气相色谱-质谱联用法中气相色谱的参数为采用规格为100m×0.25mm，0.20μm的HP-88型气相色谱柱；升温程序为初始温度60℃，保持5min，以8℃/min升至160℃，然后以4℃/min升至200℃，保持5min，最后以3℃/min升至240℃，保持5min；进样器为Thermo Scientific TriPlus RSH自动进样器；进样口温度为200℃；恒流速模式为1mL/min；进样量为1μL；分流比为10:1；载气为N2。
优选或可选地，步骤（2）中，气相色谱-质谱联用法中质谱的参数为采用电子轰击离子源模式；离子源温度280℃；传输线温度240℃；四级杆温度150℃；四级杆质量扫描范围m/z35-400。
优选或可选地，步骤（3）中Yb的计算方法为Yb=0.016×CC12＋0.977×CC4＋0.174×CC20＋0.152×CC20:3n6＋0.377×CC15:1＋0.128×CC20:4n6＋0.014×CC14＋0.647×CC6＋0.084×CC18：3n6＋0.098×CC22:1n9＋0.013×CC16:1＋0.043×CC22:6n3＋0.131×CC8＋0.304×CC24:0＋0.006×CC16＋0.815×CC21＋0.002×CC18:1n9c，其中，各标志物浓度的单位为ppm。
有益效果
本发明通过提取检测婴幼儿摄食的母乳或乳制品样品中特定的脂肪酸的含量，以实现对于该婴幼儿的精细动作发育状况的评价，并可根据评价结果推测该婴幼儿在同龄人中的发育水平，以便及时的对母体给出膳食和营养学的相关建议。该方法成本低廉，简便有效，适用于大规模的评价工作。
附图说明
图1为37种脂肪酸甲酯标品总离子流图；
图2为编号为10256的母乳样本的37种脂肪酸甲酯总离子流图。
具体实施方式
为了便于理解本发明，下面将结合说明书附图和较佳实验例对本发明作更全面、细致地描述，但本发明的保护范围并不限于以下具体的实施例。
除非另有定义，下文中所使用的所有专业术语与本领域技术人员通常理解的含义相同。本文中所使用的专业术语只是为了描述具体实施例的目的，并不是旨在限制本发明的保护范围。
除非另有特别说明，本发明中用到的各种原材料、试剂、仪器和设备等均可通过市场购买得到或者可通过现有方法制备得到。
实施例1
本发明实施例提供了一种评价婴幼儿精细动作发育状况的方法。
本实施例中检测样品的为母乳。所述母乳选择不吸烟，没有已知疾病的临床迹象，于采集前四周内无服用抗生素的记录，且足月分娩，无乳腺炎的症状和体征的母体提供的母乳。
采集时，使用预先清洗过的吸奶器从一侧或两侧乳房收集30mL乳汁。
母乳采集的时间点以分娩当日为起点，分别采集初乳一次（产后5天±2天），过渡乳一次（10天±3天），成熟乳一次（2月±3天），成熟乳一次（4月±3天），成熟乳一次（6月±3天）,成熟乳一次（8月±3天）,晚乳一次（10月±3天），共取乳7次，对于母乳采集过程中的漏采、采集不足的母体，均予剔除。
母乳采集的时间统一设定为上午9~11时，所有供乳母体均于9:00时后不得摄入任何食物和液体，并于间隔上一次喂奶2小时后，再进行收集。乳样常温(10℃-30℃)放置时间不得超过6小时，检测前摇匀。
采集到的母乳置于消毒容器内，并在-20℃下进行保存。
在采集母乳样品的同时，收集各供乳母体的年龄、身高、体重、24小时膳食回忆、饮食习惯、维生素和/或矿物质补充剂的使用情况、婴儿出生状况信息。
同时采用ASQ问卷的形式，跟踪各母体的婴幼儿在母体采集周期内的精细动作发育状况，得到各样品对应的婴幼儿的实际精细动作评分Yb。
通过上述的方法收集样本共377个。
在检测前，对母乳样品采用盐酸-甲醇法对脂肪酸进行甲酯化预处理，以便于后续的进一步测定，具体的实施方法为：
取200μL母乳，加入0.5mol/L的盐酸-甲醇溶液5mL，加入2mL正己烷，2mL甲醇，振荡混匀，在80℃下恒温水浴振荡2h，然后用流动水冷却至室温，再加入2mL去离子水，振荡混匀，于5000rpm下冷冻离心5min，取1mL上层清液（酯层溶液）于进样瓶，用于后续的气相色谱-质谱联用法，在检测前将样品在-20℃下保存。
将样品预处理完成后，进行气相色谱-质谱联用法检测，本实施例中，所述色谱的条件如下：色谱柱：HP-88气相色谱柱(100m×0.25mm，0.20μm)；升温程序：初始温度60℃，保持5min，以8℃/min升至160℃，然后以4℃/min升至200℃，保持5min，最后以3℃/min升至240℃，保持5min；进样器：ThermoScientificTriPlusRSH自动进样器；进样口温度：200℃；恒流速模式：1mL/min；进样量：1μL；分流比：10:1；载气：N2。
质谱条件如下：电子轰击离子源；离子源温度280℃；传输线温度240℃；四级杆温度150℃；四级杆质量扫描范围m/z35-400。
作为示例，图1为测定母乳中37种主要脂肪酸甲酯标品的总离子流图，图2为测定过程中编号为10256的母乳样本的总离子流图。
测定全部的377个样品后，随机选取其中的207个通过SPSS21统计软件对样品的脂肪酸数据和样品对应的Yb评分进行相关性分析，选取最低显著性差异在95%置信水平(p＜0.05)的脂肪酸作为标志物组成标志物组合，并通过单因素方差分析及多元线性回归分析，建立标准曲线及数据模型。
其中，SPSS分析结果如表1所示：
表1SPSS分析脂肪酸结果表
| 脂肪酸 | B | Sig. |
| C12 | 0.016 | 0.02 |
| C4 | 0.977 | 0.01 |
| C20 | 0.174 | 0.02 |
| C20:3n6 | 0.152 | 0.02 |
| C15:1 | 0.377 | 0.01 |
| C20:4n6 | 0.128 | 0.02 |
| C14 | 0.014 | 0.02 |
| C6 | 0.647 | 0.05 |
| C18：3n6 | 0.084 | 0.02 |
| C22:1n9 | 0.098 | 0.02 |
| C16:1 | 0.013 | 0.02 |
| C22:6n3 | 0.043 | 0.05 |
| C8 | 0.131 | 0.05 |
| C24:0 | 0.304 | 0.02 |
| C16 | 0.006 | 0.02 |
| C21 | 0.815 | 0.05 |
| C18:1n9c | 0.002 | 0.02 |
根据表1，建立的数据模型为Yb=0.016×CC12＋0.977×CC4＋0.174×CC20＋0.152×CC20:3n6＋0.377×CC15:1＋0.128×CC20:4n6＋0.014×CC14＋0.647×CC6＋0.084×CC18：3n6＋0.098×CC22:1n9＋0.013×CC16:1＋0.043×CC22:6n3＋0.131×CC8＋0.304×CC24:0＋0.006×CC16＋0.815×CC21＋0.002×CC18:1n9c，其中各标志物的浓度单位均为ppm。
进一步的，统计各样品的Yb得分设置维度，结果如表2所示：
表2Yb得分维度表
| 维度 | Yb |
| 0 | -3.73 |
| 25% | 20.38728 |
| 50% | 39.68725 |
| 75% | 75.67696 |
| 100% | 219.4236 |
。
即将通过模型计算得到的Yb得分带入到该维度表内即可得到该样品来源母体的婴幼儿的精细动作发育状况在整体婴幼儿中的水平，当该水平过低时，如低于50%或25%，即可建议对母体进行及时的膳食和营养学干预措施。
实施例2
以实施例1中测定的377个样本中剩余的156个样本作为验证集对实施例1中得到的模型进行验证。以模型计算的Yb得分与实际样本的Yb得分在同一维度内计为正确结果。
经验证集验证，实施例1中提供的模型准确率为91.9%，灵敏度为92.3%。上述结果表明，实施例1提供的模型具备良好的准确性和灵敏度。
即通过该模型，通过测定母乳样品或喂养使用的乳制品样品中标注物组各标志物脂肪酸的含量即可有效的预测或评价该样品来源的婴幼儿或喂养对象的精细动作发育状况，以便及时的在膳食和营养学方面进行建议和采取干预措施。
本发明提供的方法和模型成本低廉，简便有效，适用于大规模的评价工作，具有良好的应用前景。
最后应说明的是：以上实施例仅用以说明本发明的技术方案，而非对其限制；尽管参照前述实施例对本发明进行了详细的说明，本领域的普通技术人员应当理解：其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换；而这些修改或者替换，并不使相应技术方案的本质脱离本发明各实施例技术方案的精神和范围。
Claims (4)
1.一种采用标志物组评价婴幼儿精细动作发育状况的方法，其特征在于，按下述步骤依次进行：
（1）收集样品，并预处理，制得待测样品；所述待测样品为母乳，样品的预处理方法为采用盐酸-甲醇法对样品进行甲酯化预处理；
（2）采用气相色谱-质谱联用法测定待测样品中各标志物组标志物的含量；所述标志物组由以下脂肪酸组成：C12，C4，C20，C20:3n6，C15:1，C20:4n6，C14，C6，C18:3n6，C22:1n9，C16:1，C22:6n3，C8，C24:0，C16，C21，C18:1n9c；
（3）根据测定的各标志物含量计算该样品的得分Yb；Yb的计算方法为Yb=0.016×CC12＋0.977×CC4＋0.174×CC20＋0.152×CC20:3n6＋0.377×CC15:1＋0.128×CC20:4n6＋0.014×CC14＋0.647×CC6＋0.084×CC18:3n6＋0.098×CC22:1n9＋0.013×CC16:1＋0.043×CC22:6n3＋0.131×CC8＋0.304×CC24:0＋0.006×CC16＋0.815×CC21＋0.002×CC18:1n9c，其中，各标志物浓度的单位为ppm；
（4）根据得分Yb评价婴幼儿精细动作发育状况。
2.根据权利要求1所述的方法，其特征在于，所述盐酸-甲醇法的具体工艺为取200μL样品，加入0.5mol/L 的盐酸-甲醇溶液5mL，加入2mL正己烷，2mL 甲醇，振荡混匀，在80℃下恒温水浴振荡2h，然后用流动水冷却至室温，再加入2mL去离子水，振荡混匀，于5000rpm下冷冻离心5min，取1mL上层清液于进样瓶，即得待测样品。
3. 根据权利要求1所述的方法，其特征在于，步骤（2）中，气相色谱-质谱联用法中气相色谱的参数为采用规格为100m×0.25mm，0.20μm的HP-88型气相色谱柱；升温程序为初始温度60℃，保持5min，以8℃/min 升至160℃，然后以4℃/min 升至200℃，保持5min，最后以3℃/min 升至240℃，保持5min；进样器为Thermo Scientific TriPlus RSH 自动进样器；进样口温度为200℃；恒流速模式为1mL/min；进样量为1μL；分流比为10:1；载气为N2。
4. 根据权利要求1所述的方法，其特征在于，步骤（2）中，气相色谱-质谱联用法中质谱的参数为采用电子轰击离子源模式；离子源温度280℃；传输线温度240℃；四级杆温度150℃；四级杆质量扫描范围m/z 35-400。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310119104.3A CN116008438B (zh) | 2023-02-07 | 2023-02-07 | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 |
| PCT/CN2023/101767 WO2024164482A1 (zh) | 2023-02-07 | 2023-06-21 | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310119104.3A CN116008438B (zh) | 2023-02-07 | 2023-02-07 | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116008438A CN116008438A (zh) | 2023-04-25 |
| CN116008438B true CN116008438B (zh) | 2023-06-06 |
Family
ID=86033644
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310119104.3A Active CN116008438B (zh) | 2023-02-07 | 2023-02-07 | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN116008438B (zh) |
| WO (1) | WO2024164482A1 (zh) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116008438B (zh) * | 2023-02-07 | 2023-06-06 | 北京三元食品股份有限公司 | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115267098A (zh) * | 2022-07-29 | 2022-11-01 | 北京三元食品股份有限公司 | 一种根据脂质成分评价乳类产品的方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2488283C2 (ru) * | 2007-11-01 | 2013-07-27 | Энзимотек Лтд. | Липидная смесь для детского питания |
| CA2663327C (en) * | 2009-04-20 | 2017-03-07 | Women's & Children's Health Research Institute | Methods and compositions for promoting the neurological development of an infant |
| CA2812852A1 (en) * | 2010-10-21 | 2012-04-26 | Swedish Orphan Biovitrum Ab (Publ) | Method to increase the absorption of unsaturated fatty acids by human infants |
| CN104165955B (zh) * | 2014-06-27 | 2016-03-09 | 浙江大学 | 母乳脂肪酸谱数据库的建立方法 |
| CN106706610A (zh) * | 2015-11-12 | 2017-05-24 | 北京鑫华乐源科技发展有限公司 | 含α-亚麻酸鸡蛋的快速鉴别技术 |
| AU2016374161A1 (en) * | 2015-12-14 | 2018-04-19 | Nestec S.A. | Nutritional compositions and infant formulas to promote myelination in the brain |
| CA3003651A1 (en) * | 2016-03-30 | 2017-10-05 | Nestec S.A. | Compositions comprising fatty acids and their use |
| CN112823646A (zh) * | 2019-11-20 | 2021-05-21 | 内蒙古伊利实业集团股份有限公司 | 组合物、食品或药品及其用途 |
| EP4171564A4 (en) * | 2020-06-25 | 2024-06-26 | Woolsey Pharmaceuticals, Inc. | METHODS FOR TREATING NEURODEVELOPMENTAL DISORDERS |
| JP2024507187A (ja) * | 2021-02-10 | 2024-02-16 | 中科微智（北京）生物科技有限公司 | ビフィドバクテリウム・ラクティス及びその使用 |
| CN113707273A (zh) * | 2021-09-24 | 2021-11-26 | 中国疾病预防控制中心营养与健康所 | 婴幼儿膳食智能化评价方法、系统及设备 |
| CN116008438B (zh) * | 2023-02-07 | 2023-06-06 | 北京三元食品股份有限公司 | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 |
-
2023
- 2023-02-07 CN CN202310119104.3A patent/CN116008438B/zh active Active
- 2023-06-21 WO PCT/CN2023/101767 patent/WO2024164482A1/zh unknown
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115267098A (zh) * | 2022-07-29 | 2022-11-01 | 北京三元食品股份有限公司 | 一种根据脂质成分评价乳类产品的方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116008438A (zh) | 2023-04-25 |
| WO2024164482A1 (zh) | 2024-08-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115825294B (zh) | 一种用于评价婴幼儿沟通能力和发育状况的标志物组及应用 | |
| Sun et al. | Evaluation of triacylglycerol composition in commercial infant formulas on the Chinese market: A comparative study based on fat source and stage | |
| Smit et al. | Estimated biological variation of the mature human milk fatty acid composition | |
| Van de Vijver et al. | Association between trans fatty acid intake and cardiovascular risk factors in Europe: the TRANSFAIR study | |
| CN116008438B (zh) | 一种用于评价婴幼儿精细动作发育状况的标志物组及应用 | |
| Nishimura et al. | Dietary polyunsaturated fatty acid intake during late pregnancy affects fatty acid composition of mature breast milk | |
| Perrin et al. | A cross-sectional study of fatty acids and brain-derived neurotrophic factor (BDNF) in human milk from lactating women following vegan, vegetarian, and omnivore diets | |
| Scopesi et al. | Maternal dietary PUFAs intake and human milk content relationships during the first month of lactation | |
| EP4339610A1 (en) | Method for evaluating milk products according to lipidic components | |
| Mitoulas et al. | Infant intake of fatty acids from human milk over the first year of lactation | |
| Hakola et al. | Maternal fatty acid intake during pregnancy and the development of childhood overweight: a birth cohort study | |
| IL208378A (en) | Infant feeding formula and use thereof | |
| Simões-Wüst et al. | Organic food consumption during pregnancy and its association with health-related characteristics: the KOALA Birth Cohort Study | |
| Petersohn et al. | Maternal diet and human milk composition: an updated systematic review | |
| Özbey et al. | The relationship between dietary inflammatory index, pulmonary functions and asthma control in asthmatics | |
| Huynh et al. | Maternal omega-3 supplementation during pregnancy, but not childhood supplementation, reduces the risk of food allergy diseases in offspring | |
| US20240264179A1 (en) | Marker set for evaluating the development of infant’s communication ability and use thereof | |
| Ding et al. | Association between dietary fatty acid patterns based on principal component analysis and fatty acid compositions of serum and breast milk in lactating mothers in Nanjing, China | |
| Penev et al. | Influence of heat stress and physiological indicators related to it on health lipid indices in milk of holstein-friesian cows | |
| YOUNG et al. | Fatty acid compositions of colostrum, cord blood, maternal blood and major infant formulas in Japan | |
| Meneses et al. | Essential and long-chain polyunsaturated fatty acid status and fatty acid composition of breast milk of lactating adolescents | |
| Hegsted et al. | Diet and serum lipid concentrations: where are we? | |
| Babin et al. | Alpha linolenic acid in cholesterol esters: a marker of alphalinolenic acid intake in newborns | |
| Barros et al. | Cord blood essential fatty acid and alpha-tocopherol in full-term newborns in a Northeast Brazil area | |
| Rochman et al. | EFFECT OF RECOVERY SUPPLEMENTARY FOOD PROGRAM ON NUTRITIONAL STATUS OF STUNTING TODDLERS AT CIMAHI CITY HEALTH CENTER APRIL-JULY 2021 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 40085644 Country of ref document: HK |