CN115982034B - Test method of virtual terminal of carrier construction system, storage medium and electronic equipment - Google Patents
Test method of virtual terminal of carrier construction system, storage medium and electronic equipment Download PDFInfo
- Publication number
- CN115982034B CN115982034B CN202211731228.9A CN202211731228A CN115982034B CN 115982034 B CN115982034 B CN 115982034B CN 202211731228 A CN202211731228 A CN 202211731228A CN 115982034 B CN115982034 B CN 115982034B
- Authority
- CN
- China
- Prior art keywords
- carrier
- meets
- vector
- sequence
- expectations
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000010998 test method Methods 0.000 title claims abstract description 17
- 238000010276 construction Methods 0.000 title abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 30
- 238000004458 analytical method Methods 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 16
- 238000001514 detection method Methods 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims description 47
- 239000013598 vector Substances 0.000 claims description 39
- 230000015654 memory Effects 0.000 claims description 35
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 239000003550 marker Substances 0.000 claims description 14
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 238000003776 cleavage reaction Methods 0.000 claims description 6
- 230000007017 scission Effects 0.000 claims description 6
- 230000014509 gene expression Effects 0.000 claims description 4
- 241000701161 unidentified adenovirus Species 0.000 claims description 4
- 238000003208 gene overexpression Methods 0.000 claims description 3
- 230000006870 function Effects 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 108020004705 Codon Proteins 0.000 description 4
- 238000004891 communication Methods 0.000 description 4
- 108700026244 Open Reading Frames Proteins 0.000 description 3
- 238000004590 computer program Methods 0.000 description 3
- 230000008878 coupling Effects 0.000 description 3
- 238000010168 coupling process Methods 0.000 description 3
- 238000005859 coupling reaction Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000014616 translation Effects 0.000 description 2
- 101100322245 Caenorhabditis elegans des-2 gene Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 108010030074 endodeoxyribonuclease MluI Proteins 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Abstract
The application provides a test method of a carrier construction system virtual terminal, a computer storage medium and electronic equipment, wherein the virtual terminal comprises a system inlet, a plurality of operation buttons and a system level, and the test method comprises the following steps: s1, detecting whether a system level meets a set requirement or not; s2, detecting whether the system inlet and the plurality of operation buttons are normal or not; s3, testing the constructed carrier to judge whether the operation of the insertable element, the name template of the carrier and the raw material analysis tool meets the expectations; s4, outputting a test result when the detection results of the step S1, the step S2 and the step S3 are normal or meet the set requirements or expectations. According to the testing method provided by the embodiment of the application, whether the virtual terminal of the carrier construction system meets the use requirement can be rapidly and effectively detected before a new carrier system is on line, so that manpower, time and hardware resources are effectively saved, and the testing efficiency is greatly improved.
Description
Technical Field
The present application relates to the field of system detection, and more particularly, to a method for testing a virtual terminal of a carrier building system, a computer storage medium, and an electronic device.
Background
The automatic test is to execute a related test according to the flow of a preset test case, and to check whether the actual result is consistent with the expected result.
With the rapid development of biology, a great deal of manpower and time are required to test whether a newly built carrier system meets the requirements in the process of uploading a new carrier system, and the traditional manual testing process is not suitable for the development requirement.
Disclosure of Invention
In order to solve the technical problems, the application provides a method for testing a virtual terminal of a carrier construction system, a computer storage medium and electronic equipment, which can save manpower, time or hardware resources and improve test efficiency.
According to the test method of the carrier construction system virtual terminal, which comprises a system inlet, a plurality of operation buttons and a system level, the test method comprises the following steps:
s1, detecting whether the system level meets a set requirement or not;
s2, detecting whether the system inlet and a plurality of operation buttons are normal or not;
s3, testing the constructed carrier to judge whether the operation of the insertable element, the name template of the carrier and the raw material analysis tool meets the expectations;
s4, outputting a test result when the detection results of the step S1, the step S2 and the step S3 are normal or meet the set requirements or expectations.
According to the test method of the virtual terminal of the carrier construction system, provided by the embodiment of the application, the system level, the system entrance and a plurality of operation buttons of the virtual terminal and the constructed carrier are tested step by step, and whether the operation of the insertable element, the name template of the carrier and the raw material analysis tool accords with the expectation is judged, so that before a new carrier system is online, whether the virtual terminal of the carrier construction system accords with the use requirement can be quickly and effectively detected, the manpower, the time and the hardware resources are effectively saved, and the test efficiency is greatly improved.
According to one embodiment of the application, a method of determining whether an insertable element meets expectations includes: judging whether a framework of the insertable element contains any one of an insertable option Promoter, ORF and Marker, and if the framework contains any one of the insertable options, judging that the insertable element meets the expectations.
According to one embodiment of the application, the test method further comprises: the method for detecting the skeleton to judge whether the insertable element meets the expectations or not comprises the following steps: judging whether the insertable element uses the skeletons of des3d and des2d, and if the insertable element uses the skeletons of des3d and des2d, judging that the insertable element meets the expectations.
According to one embodiment of the application, the method for judging whether the name template of the carrier meets the expectations is as follows:
judging whether the name of the carrier accords with the following formula:
p Ad5/F35[Exp]-Marker-Promoter>ORF
wherein p denotes that all carrier names begin with the letter "p"; ad5/F35 means that the vector backbone is a human Ad5/F35 chimeric adenovirus vector, exp means that the biological application is gene overexpression; marker represents a mark; promoter means a Promoter driving expression of a target gene; ORF represents the gene of interest; "-" indicates a connector between elements; ">" means that the ORF is located behind the promoter.
According to one embodiment of the present application, a method of determining whether a feedstock analysis tool meets expectations includes:
judging whether the skeleton of the insertable element contains the insertable option according to a carrier;
detecting the skeleton by using the raw material analysis tool;
judging whether the enzyme cutting site of the insertable element meets the requirement or not;
determining whether the sequence of the vector meets expectations.
According to one embodiment of the application, the feedstock analysis tools include Destination Vector, pUp, pDOWN and pTail, and Destination Vector are used to detect the scaffold.
According to one embodiment of the application, destination Vector is used to detect the cleavage site of the insertable element.
According to one embodiment of the application, the method for determining whether the sequence of the vector corresponds to the expected sequence comprises:
providing a standard genebank file, obtaining a fixed sequence and an expected position of the pluggable element in the standard genebank file;
and obtaining the sequence and the position of the constructed vector, comparing the sequence and the position of the vector with the fixed sequence and the expected position, and judging that the sequence of the vector accords with the expected position when the sequence and the position of the vector are matched with the fixed sequence and the expected position.
In a second aspect, embodiments of the present application provide a computer storage medium comprising one or more computer instructions which, when executed, implement a method as described in the above embodiments.
An electronic device according to an embodiment of the third aspect of the present application includes a memory for storing one or more computer instructions and a processor; the processor is configured to invoke and execute the one or more computer instructions to implement the method as described in any of the embodiments above.
Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.
Drawings
The foregoing and/or additional aspects and advantages of the application will become apparent and may be better understood from the following description of embodiments taken in conjunction with the accompanying drawings in which:
fig. 1 is a flowchart of a method for testing a virtual terminal of a carrier build system according to an embodiment of the present application;
fig. 2 is a schematic diagram of an electronic device according to an embodiment of the application.
Reference numerals:
an electronic device 300;
a memory 310; an operating system 311; an application 312;
a processor 320; a network interface 330; an input device 340; a hard disk 350; and a display device 360.
Detailed Description
Embodiments of the present application are described in detail below, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to like or similar elements or elements having like or similar functions throughout. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the application.
In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present application and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application. Furthermore, features defining "first", "second" may include one or more such features, either explicitly or implicitly. In the description of the present application, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.
The terminology mentioned in the present application will be explained first.
Gene sequence: the primary structure of a nucleic acid uses a series of letters representing the primary structure of a real or hypothetical DNA molecule carrying genetic information. Each letter represents a nucleobase, two bases form a base pair, the pairing rules of which are fixed, a=t, c≡g. Three adjacent base pairs form one codon. One codon corresponds to one amino acid, and different amino acids synthesize different proteins. The base pairing rules are critical in DNA replication and protein synthesis.
Promter: the promoter is a DNA sequence located upstream of the 5' end of the structural gene, and can activate RNA polymerase to accurately combine with template DNA and have specificity of transcription initiation. Promoters (promoles) act like "switches" to determine the activity of a gene.
ORF open reading frame (open reading frame, ORF), which is the normal nucleotide sequence of a structural gene, the reading frame from the start codon to the stop codon may encode the complete polypeptide chain without a stop codon interrupting translation.
Marker: a marker, a region on the chromosome that can be recognized (e.g., restriction enzyme cleavage point, gene position, etc.). The inheritance of the marker can be detected. The marker may be a part (such as a gene) having an expression function on a chromosome, or may be a part which does not encode a protein function but whose genetic characteristics can be detected.
Destination Vector: the final vector is the vector that the customer finally needs to construct.
puc, pDown and pTail: all refer to entry cloning vectors that are split by att sites.
The following describes a method for testing a virtual terminal of a carrier build system according to an embodiment of the present application in detail with reference to the accompanying drawings.
The test method of the carrier construction system virtual terminal according to the embodiment of the application comprises the following steps as shown in fig. 1:
s1, detecting whether a system level meets a set requirement or not;
s2, detecting whether the system inlet and the plurality of operation buttons are normal or not;
s3, testing the constructed carrier to judge whether the operation of the insertable element, the name template of the carrier and the raw material analysis tool meets the expectations;
s4, outputting a test result when the detection results of the step S1, the step S2 and the step S3 are normal or meet the set requirements or expectations.
In other words, the testing method according to the embodiment of the application can be used for testing a new carrier construction system which needs to be on line so as to judge whether the system meets the use requirement. The virtual terminal of the test system comprises a system inlet, a plurality of operation buttons and a system level, the operation buttons can comprise an insert element button and a click completion button, the test method comprises the steps of checking the system level, checking the system inlet and the plurality of operation buttons and testing the constructed carrier, wherein the system inlet, the operation buttons and the system level and the checking standards and the like of the system inlet, the operation buttons and the system level are all understood and easily realized by a person skilled in the art, and therefore, the detection of the system inlet, the operation buttons and the system level is not described in detail, but the process of testing the constructed carrier is described in an important way.
The process of testing the constructed carrier includes detecting the operation of the pluggable element, the name template of the carrier, and the raw material analysis tool to determine if the operation of the pluggable element, the name template of the carrier, and the raw material analysis tool are in compliance with expectations. Only when the system entrance, the operation button and the system level contained in the virtual system meet the set requirements, and the operation of the insertable element, the name template of the carrier and the raw material analysis tool also meet the set requirements, a test result is output, which indicates that the carrier construction system needing to be on line meets the requirements, and the system can be used on line.
According to the test method of the virtual terminal of the carrier construction system, disclosed by the embodiment of the application, the system level, the system entrance and a plurality of operation buttons of the virtual terminal and the constructed carrier are tested step by step, and whether the operation of the pluggable element, the name template of the carrier and the raw material analysis tool accords with the expectation or not is judged, so that before a new carrier system is online, whether the virtual terminal of the carrier construction system accords with the use requirement or not can be rapidly and effectively detected, the manpower, the time and the hardware resources are effectively saved, and the test efficiency is greatly improved.
According to one embodiment of the application, a method of determining whether an insertable element meets expectations includes: judging whether the skeleton of the insertable element contains any one of the insertable options Promoter, ORF and Marker, and if the skeleton contains any one of the insertable options, judging that the insertable element meets the expectations.
In other words, the insertable element has a skeleton, if the skeleton contains a Promoter therein; an ORF; any one or more of the three pluggable options of the Marker, the pluggable element is considered to meet the set requirements, and the next detection can be performed.
In some embodiments of the application, the test method further comprises: the method for detecting the skeleton and judging whether the insertable element meets the expectations or not comprises the following steps:
determining whether the pluggable element uses the skeletons of des3d and des2d, and if the pluggable element uses the skeletons of des3d and des2d, determining that the pluggable element meets the expectations.
That is, there are two expected outcomes that the backbone of the insertable element may exist when analyzing the insertable element, namely:
first kind: determining whether the insertable elements all use the skeleton of des3 d; (pAd. Des3d (Ad 5-F35)). Wherein p denotes that all vector names begin with the letter "p", des3d denotes the type of backbone, and Ad5-F35 denotes that the vector backbone is a human Ad5/F35 chimeric adenovirus vector. In the judging method, firstly, one insertable option of the insertable element is selected, and if no insertable option Marker or more than two ORFs are contained in the insertable element, the insertable element is not provided with a skeleton of des3 d.
Second kind: determining whether the insertable elements all use the skeleton of des2 d; (pAd. Des2d (Ad 5-F35)).
The insertable element is expected when the detection result of the insertable element corresponds to the first and second conditions, and the insertable element is not expected when the detection result of the insertable element does not correspond to the first and second conditions.
Optionally, according to an embodiment of the present application, the method for determining whether the name template of the carrier meets the expectations is: judging whether the name of the carrier accords with the following formula:
p Ad5/F35[Exp]-Marker-Promoter>ORF
wherein p denotes that all carrier names begin with the letter "p"; ad5/F35 means that the vector backbone is a human Ad5/F35 chimeric adenovirus vector, exp means that the biological application is gene overexpression; marker represents a mark; promoter means a Promoter driving expression of a target gene; ORF represents the gene of interest; "-" indicates a connector between elements; ">" means that the ORF is located behind the promoter.
And if the name of the carrier accords with the naming mode and the naming sequence of the formula, the name template of the carrier accords with the expectation.
In some embodiments of the application, a method of determining whether a feedstock analysis tool meets expectations includes:
judging whether the framework of the insertable element contains an insertable option according to the carrier;
detecting the skeleton by adopting a raw material analysis tool;
judging whether the enzyme cutting site of the insertable element meets the requirement;
determining whether the sequence of the vector meets the expectations.
Specifically, the raw material analysis tool may include, for example, destination Vector, pUp, pDOWN and pTail, and sequences meeting any of the criteria of the raw material analysis tool may be automatically resolved.
Wherein, according to the carrier, it can be determined that the Marker is inserted into the insertable element, and if the skeleton of the insertable element accords with the skeleton of the usage des3d, the insertable element is compared with Destination Vector of the raw material analysis tool, and it can be determined that the skeleton of the insertable element accords with the expectation.
According to one embodiment of the present application, the method of determining whether a feedstock analysis tool meets expectations further comprises: and Destination Vector is adopted to detect the enzyme cutting site of the insertable element, so as to judge whether the enzyme cutting site of the insertable element meets the requirement.
Specifically, the method for judging whether the enzyme cutting site of the insertable element sequence meets the requirement comprises the following steps:
pUp corresponds to promter;
pDown corresponds to ORF;
pTail is mapped to markers, for example: the Restriction Enzymesb sequence of Materials > pUp is obtained first, the sequence is created in Snapgene Viewer, and the corresponding enzyme (MluI+SacI) is selected to obtain the expected cleavage site (Location) and fragment sequence length (Fragments). It should be noted that the sequence is a series of bases, a certain cleavage site can be formed, the series of sequences can be cleaved by selecting the corresponding enzyme, and if the series of sequences are not aligned, the enzyme cannot cleave the series of sequences.
And comparing the expected enzyme cutting site with the enzyme cutting site of the insertable element, namely comparing the enzyme cutting site with the fragment sequence length, and if the comparison result is consistent, considering the enzyme cutting site of the insertable element to be consistent with the expected enzyme cutting site. It should be noted that if there are 1-2 base differences in the alignment, these differences are acceptable and the cleavage site of the insertable element is considered to be expected.
In some embodiments of the application, the method of determining whether the sequence of the vector corresponds to an expected sequence comprises:
providing a standard genebank file, and obtaining the expected position of the fixed sequence and the insertable element in the standard genebank file;
and obtaining the sequence and the position of the constructed carrier, comparing the sequence and the position of the carrier with the fixed sequence and the expected position, and judging that the sequence of the carrier accords with the expected when the sequence and the position of the carrier are matched with the fixed sequence and the expected position.
Specifically, the fixed sequence and the specific position of the insertable element in the genebank file may be obtained from a standard genebank file, which may be used as the desired result. For example, the first n of the insertable element represents an insertable Promoter, the second n represents an insertable ORF, and the third n represents an insertable Marker. The vector generated by the vector construction system must contain sequences of sequence 1, sequence 2, sequence 3 and sequence 4.
And then obtaining the sequence of the constructed vector, and judging whether the actual sequence and the position are consistent with the expected sequence 1, sequence 2, sequence 3 and sequence 4 of the genebank file.
When all positions and sequences meet the expected conditions, the detection result is satisfied, and the carrier construction system can be used on line.
In addition, the application also provides a computer storage medium, which comprises one or more computer instructions, and the one or more computer instructions realize the test method of the virtual terminal of the carrier construction system in any one of the above steps when being executed.
That is, the computer storage medium stores a computer program that, when executed by the processor, causes the processor to execute the test method of the virtual terminal of the carrier build system of any one of the above.
As shown in fig. 2, an embodiment of the present application provides an electronic device 300, including a memory 310 and a processor 320, where the memory 310 is configured to store one or more computer instructions, and the processor 320 is configured to invoke and execute the one or more computer instructions, thereby implementing any of the methods described above.
That is, the electronic device 300 includes: a processor 320 and a memory 310, in which memory 310 computer program instructions are stored which, when executed by the processor, cause the processor 320 to perform any of the methods described above.
Further, as shown in fig. 2, the electronic device 300 also includes a network interface 330, an input device 340, a hard disk 350, and a display device 360.
The interfaces and devices described above may be interconnected by a bus architecture. The bus architecture may be a bus and bridge that may include any number of interconnects. One or more Central Processing Units (CPUs), represented in particular by processor 320, and various circuits of one or more memories, represented by memory 310, are connected together. The bus architecture may also connect various other circuits together, such as peripheral devices, voltage regulators, and power management circuits. It is understood that a bus architecture is used to enable connected communications between these components. The bus architecture includes, in addition to a data bus, a power bus, a control bus, and a status signal bus, all of which are well known in the art and therefore will not be described in detail herein.
The network interface 330 may be connected to a network (e.g., the internet, a local area network, etc.), and may obtain relevant data from the network and store the relevant data in the hard disk 350.
The input device 340 may receive various instructions from an operator and transmit the instructions to the processor 320 for execution. The input device 340 may include a keyboard or pointing device (e.g., a mouse, a trackball, a touch pad, or a touch screen, among others).
The display device 360 may display results obtained by the processor 320 executing instructions.
The memory 310 is used for storing programs and data necessary for the operation of the operating system, and data such as intermediate results in the calculation process of the processor 320.
It will be appreciated that memory 310 in embodiments of the application may be volatile memory or nonvolatile memory, or may include both volatile and nonvolatile memory. The nonvolatile memory may be Read Only Memory (ROM), programmable Read Only Memory (PROM), erasable Programmable Read Only Memory (EPROM), electrically Erasable Programmable Read Only Memory (EEPROM), or flash memory, among others. Volatile memory can be Random Access Memory (RAM), which acts as external cache memory. The memory 310 of the apparatus and methods described herein is intended to comprise, without being limited to, these and any other suitable types of memory.
In some implementations, the memory 310 stores the following elements, executable modules or data structures, or a subset thereof, or an extended set thereof: an operating system 311 and applications 312.
The operating system 311 includes various system programs, such as a framework layer, a core library layer, a driver layer, and the like, for implementing various basic services and processing hardware-based tasks. The application programs 312 include various application programs such as a Browser (Browser) and the like for implementing various application services. A program implementing the method of the embodiment of the present application may be included in the application program 312.
The method disclosed in the above embodiment of the present application may be applied to the processor 320 or implemented by the processor 320. Processor 320 may be an integrated circuit chip with signal processing capabilities. In implementation, the steps of the above method may be performed by integrated logic circuitry in hardware or instructions in software in processor 320. The processor 320 may be a general purpose processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), an off-the-shelf programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic, or discrete hardware components, which may implement or perform the methods, steps, and logic blocks disclosed in embodiments of the present application. A general purpose processor may be a microprocessor or the processor may be any conventional processor or the like. The steps of the method disclosed in connection with the embodiments of the present application may be embodied directly in the execution of a hardware decoding processor, or in the execution of a combination of hardware and software modules in a decoding processor. The software modules may be located in a random access memory, flash memory, read only memory, programmable read only memory, or electrically erasable programmable memory, registers, etc. as well known in the art. The storage medium is located in the memory 310 and the processor 320 reads the information in the memory 310 and in combination with its hardware performs the steps of the method described above.
It is to be understood that the embodiments described herein may be implemented in hardware, software, firmware, middleware, microcode, or a combination thereof. For a hardware implementation, the processing units may be implemented within one or more Application Specific Integrated Circuits (ASICs), digital Signal Processors (DSPs), digital Signal Processing Devices (DSPDs), programmable Logic Devices (PLDs), field Programmable Gate Arrays (FPGAs), general purpose processors, controllers, micro-controllers, microprocessors, other electronic units designed to perform the functions described herein, or a combination thereof.
For a software implementation, the techniques described herein may be implemented with modules (e.g., procedures, functions, and so on) that perform the functions described herein. The software codes may be stored in a memory and executed by a processor. The memory may be implemented within the processor or external to the processor.
In particular, the processor 320 is further configured to read the computer program and execute any of the methods described above.
In the several embodiments provided in the present application, it should be understood that the disclosed methods and apparatus may be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative, e.g., the division of the units is merely a logical function division, and there may be additional divisions when actually implemented, e.g., multiple units or components may be combined or integrated into another system, or some features may be omitted or not performed. Alternatively, the coupling or direct coupling or communication connection shown or discussed with each other may be an indirect coupling or communication connection via some interfaces, devices or units, which may be in electrical, mechanical or other form.
In addition, each functional unit in the embodiments of the present application may be integrated in one processing unit, or each unit may be physically included separately, or two or more units may be integrated in one unit. The integrated units may be implemented in hardware or in hardware plus software functional units.
The integrated units implemented in the form of software functional units described above may be stored in a computer readable storage medium. The software functional unit is stored in a storage medium, and includes several instructions for causing a computer device (which may be a personal computer, a server, or a network device, etc.) to perform part of the steps of the transceiving method according to the embodiments of the present application. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a random access Memory (Random Access Memory, RAM), a magnetic disk, or an optical disk, or other various media capable of storing program codes.
While the foregoing is directed to the preferred embodiments of the present application, it will be appreciated by those skilled in the art that various modifications and adaptations can be made without departing from the principles of the present application, and such modifications and adaptations are intended to be comprehended within the scope of the present application.
Claims (7)
1. A method for testing a virtual terminal of a carrier build system, the virtual terminal comprising a system portal, a plurality of operation buttons, and a system hierarchy, the method comprising the steps of:
s1, detecting whether the system level meets a set requirement or not;
s2, detecting whether the system inlet and a plurality of operation buttons are normal or not;
s3, testing the constructed carrier to judge whether the operation of the insertable element, the name template of the carrier and the raw material analysis tool meets the expectations;
s4, outputting a test result when the detection results of the step S1, the step S2 and the step S3 are normal or meet the set requirements or expectations;
the method for judging whether the insertable element meets the expectations comprises the following steps:
judging whether a framework of an insertable element contains any one of an insertable option Promoter, ORF and Marker, and if the framework contains any one of the insertable options, judging that the insertable element accords with the expectation;
the method for judging whether the name template of the carrier accords with the expectation comprises the following steps: judging whether the name of the carrier accords with the following formula:
p Ad5/F35[Exp]-Marker-Promoter>ORF
wherein p denotes that all carrier names begin with the letter "p"; ad5/F35 means that the vector backbone is a human Ad5/F35 chimeric adenovirus vector, exp means that the biological application is gene overexpression; marker represents a mark; promoter means a Promoter driving expression of a target gene; ORF represents the gene of interest; "-" indicates a connector between elements; "ORF is located behind the promoter;
the method for judging whether the raw material analysis tool meets the expectations comprises the following steps:
judging whether the skeleton of the insertable element contains the insertable option according to a carrier;
detecting the skeleton by using the raw material analysis tool;
judging whether the enzyme cutting site of the insertable element meets the requirement or not;
determining whether the sequence of the vector meets expectations.
2. The test method of claim 1, further comprising: the method for detecting the skeleton to judge whether the insertable element meets the expectations or not comprises the following steps:
judging whether the insertable element uses the skeletons of des3d and des2d, and if the insertable element uses the skeletons of des3d and des2d, judging that the insertable element meets the expectations.
3. The method of testing of claim 1, wherein the feedstock analysis tool comprises Destination Vector, puc, pDown and pTail, and wherein the scaffold is tested using Destination Vector.
4. A test method according to claim 3, wherein the enzyme cleavage site of the insertable element is detected using Destination Vector.
5. The method of claim 1, wherein determining whether the sequence of the vector meets the expectations comprises:
providing a standard genebank file, obtaining a fixed sequence and an expected position of the pluggable element in the standard genebank file;
and obtaining the sequence and the position of the constructed vector, comparing the sequence and the position of the vector with the fixed sequence and the expected position, and judging that the sequence of the vector accords with the expected position when the sequence and the position of the vector are matched with the fixed sequence and the expected position.
6. A computer storage medium comprising one or more computer instructions which, when executed, implement the method of any of claims 1-5.
7. An electronic device comprising a memory and a processor, characterized in that,
the memory is used for storing one or more computer instructions;
the processor is configured to invoke and execute the one or more computer instructions to implement the method of any of claims 1-5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211731228.9A CN115982034B (en) | 2022-12-30 | 2022-12-30 | Test method of virtual terminal of carrier construction system, storage medium and electronic equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211731228.9A CN115982034B (en) | 2022-12-30 | 2022-12-30 | Test method of virtual terminal of carrier construction system, storage medium and electronic equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115982034A CN115982034A (en) | 2023-04-18 |
| CN115982034B true CN115982034B (en) | 2023-11-28 |
Family
ID=85973843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211731228.9A Active CN115982034B (en) | 2022-12-30 | 2022-12-30 | Test method of virtual terminal of carrier construction system, storage medium and electronic equipment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115982034B (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105121648A (en) * | 2012-12-12 | 2015-12-02 | 布罗德研究所有限公司 | Engineering of systems, methods and optimized guide compositions for sequence manipulation |
| CN106467917A (en) * | 2016-08-30 | 2017-03-01 | 沈阳农业大学 | A kind of Fructus Fragariae Ananssae ARF4 promoter merges the carrier construction method of gus gene |
| CN110117577A (en) * | 2018-02-05 | 2019-08-13 | 中国科学院武汉物理与数学研究所 | Less toxic herpes simplex virus system and its construction method and application |
| CN111088256A (en) * | 2019-12-30 | 2020-05-01 | 云舟生物科技（广州）有限公司 | Construction method of carrier for efficiently expressing small RNA and expressing multiple small RNAs by cynomolgus monkey U6 promoter |
| CN111145836A (en) * | 2019-12-30 | 2020-05-12 | 云舟生物科技（广州）有限公司 | Method for inserting gene nucleic acid sequence, computer storage medium and electronic device |
| WO2021175298A1 (en) * | 2020-03-05 | 2021-09-10 | 浙江东方基因生物制品股份有限公司 | Novel coronavirus detection reagent and detection method |
| CN114780421A (en) * | 2022-04-29 | 2022-07-22 | 中国航发控制系统研究所 | Abnormity testing method, system and storage medium based on virtual instruction set platform |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017075294A1 (en) * | 2015-10-28 | 2017-05-04 | The Board Institute Inc. | Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction |
| EP3393522B1 (en) * | 2015-12-22 | 2021-07-07 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Improved hybrid dual recombinant aav vector systems for gene therapy |
-
2022
- 2022-12-30 CN CN202211731228.9A patent/CN115982034B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105121648A (en) * | 2012-12-12 | 2015-12-02 | 布罗德研究所有限公司 | Engineering of systems, methods and optimized guide compositions for sequence manipulation |
| CN106467917A (en) * | 2016-08-30 | 2017-03-01 | 沈阳农业大学 | A kind of Fructus Fragariae Ananssae ARF4 promoter merges the carrier construction method of gus gene |
| CN110117577A (en) * | 2018-02-05 | 2019-08-13 | 中国科学院武汉物理与数学研究所 | Less toxic herpes simplex virus system and its construction method and application |
| CN111088256A (en) * | 2019-12-30 | 2020-05-01 | 云舟生物科技（广州）有限公司 | Construction method of carrier for efficiently expressing small RNA and expressing multiple small RNAs by cynomolgus monkey U6 promoter |
| CN111145836A (en) * | 2019-12-30 | 2020-05-12 | 云舟生物科技（广州）有限公司 | Method for inserting gene nucleic acid sequence, computer storage medium and electronic device |
| WO2021175298A1 (en) * | 2020-03-05 | 2021-09-10 | 浙江东方基因生物制品股份有限公司 | Novel coronavirus detection reagent and detection method |
| CN114780421A (en) * | 2022-04-29 | 2022-07-22 | 中国航发控制系统研究所 | Abnormity testing method, system and storage medium based on virtual instruction set platform |
Non-Patent Citations (5)
| Title |
|---|
| Development and evaluation of a new recombinant protein vaccine (YidR) against Klebsiella pneumoniae infection;Marjory Xavier Rodrigues等;Vaccine;第38卷(第29期);第4640-4648页 * |
| VectorBuilder:A revolutionary online tool for DNA vectors;Marvin(Yingbin) 等;纪念DNA双股螺旋结构发现60周年盛大庆典——2013第四届中国（南京）国际DNA和基因组活动周论文集;第53页 * |
| 人MGL真核表达载体的构建及其在细胞内定位的研究;刘占英;中国优秀硕士学位论文全文数据库 基础科学辑(第1期);A006-176 * |
| 人SUMO1基因的真核表达载体构建及其RNA干扰靶点筛选;吴勇延 等;安徽农业科学;第38卷(第29期);第16134-16137页 * |
| 基于CRISPR-Cas9技术的无启动子基因组定点整合系统的构建及其体外实验研究;胡维欣;中国优秀硕士学位论文全文数据库 医药卫生科技辑(第4期);E060-115 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115982034A (en) | 2023-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Brent et al. | Recent advances in gene structure prediction | |
| Birney et al. | GeneWise and genomewise | |
| Ansong et al. | Proteogenomics: needs and roles to be filled by proteomics in genome annotation | |
| Parra et al. | Comparative gene prediction in human and mouse | |
| Meyer et al. | Gene structure conservation aids similarity based gene prediction | |
| US20140309945A1 (en) | Genome sequence alignment apparatus and method | |
| Farrer | Synima: a Synteny imaging tool for annotated genome assemblies | |
| Goodswen et al. | Evaluating high-throughput ab initio gene finders to discover proteins encoded in eukaryotic pathogen genomes missed by laboratory techniques | |
| US20140180660A1 (en) | Methods and systems for in silico design | |
| Bitton et al. | An integrated mass-spectrometry pipeline identifies novel protein coding-regions in the human genome | |
| CN111145836A (en) | Method for inserting gene nucleic acid sequence, computer storage medium and electronic device | |
| Zhong et al. | CircPrimer 2.0: a software for annotating circRNAs and predicting translation potential of circRNAs | |
| Duan et al. | A systematic evaluation of bioinformatics tools for identification of long noncoding RNAs | |
| Gou et al. | SSRMMD: a rapid and accurate algorithm for mining SSR feature loci and candidate polymorphic SSRs based on assembled sequences | |
| CN115982034B (en) | Test method of virtual terminal of carrier construction system, storage medium and electronic equipment | |
| Tao et al. | Enabling QTY server for designing water-soluble α-helical transmembrane proteins | |
| Levin et al. | Proteotranscriptomics–A facilitator in omics research | |
| Wan et al. | Computational methods for remote homolog identification | |
| KR101770962B1 (en) | A method and apparatus of providing information on a genomic sequence based personal marker | |
| Zheng et al. | MAASE: an alternative splicing database designed for supporting splicing microarray applications | |
| van Baren et al. | Using N‐SCAN or TWINSCAN to Predict Gene Structures in Genomic DNA Sequences | |
| CN115458063A (en) | Carrier recommendation method, system, computer storage medium and electronic device | |
| CN113921083B (en) | Custom sequence analysis method, computer storage medium and electronic device | |
| Mumm et al. | Multiplexed long-read plasmid validation and analysis using OnRamp | |
| JP2007207113A (en) | Genealogical tree display system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |