CN111145836A - Method for inserting gene nucleic acid sequence, computer storage medium and electronic device - Google Patents
Method for inserting gene nucleic acid sequence, computer storage medium and electronic device Download PDFInfo
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- CN111145836A CN111145836A CN201911397883.3A CN201911397883A CN111145836A CN 111145836 A CN111145836 A CN 111145836A CN 201911397883 A CN201911397883 A CN 201911397883A CN 111145836 A CN111145836 A CN 111145836A
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Abstract
The invention provides a method for inserting gene nucleic acid sequence, a computer storage medium and an electronic device, wherein the method comprises the following steps: s1, constructing a nucleic acid sequence element library; s2, selecting a carrier framework, and arranging at least one insertion site on the carrier framework; s3, obtaining site information of the insertion site selected by the user, and calling a corresponding nucleic acid sequence in the nucleic acid sequence element library according to the site information; s4, obtaining corresponding nucleic acid sequences from the nucleic acid sequence element library, and inserting the nucleic acid sequences into insertion sites corresponding to the site information; s5, editing the inserted nucleic acid sequence; s6, judging whether the nucleic acid sequence of the vector framework is in compliance, if so, generating the vector, otherwise, sending a prompt message. According to the method for inserting the gene nucleic acid sequence, the possibility of misoperation is greatly reduced, the misoperation rate is reduced, the construction efficiency is improved, and the method is more friendly to users.
Description
Technical Field
The present invention relates to the field of gene editing, and more particularly, to a method of inserting a nucleic acid sequence of a gene, a computer storage medium, and an electronic device.
Background
With the continuous development of biotechnology, people have more and more demands on carriers, which are basic materials required by biological experiments, and with the wide application of computer technology, more carrier designs are changed from traditional paper drawing designs to computer designs. The vector construction is carried out by using a computer, and a user mainly uses software to obtain an intuitive vector diagram or insert a certain nucleic acid sequence into the original vector diagram by inputting or pasting a biological sequence so as to perfect the vector. At present, systems for designing vectors by computers are increasing, such as SnapGene, Vector NT, etc.
However, in the current vector construction system in the market, a user needs to refer to a large amount of documents, analyze and verify before constructing the vector, so that a lot of time is spent, and due to the complexity of a vector sequence, the whole vector loses biological effectiveness as long as one base is wrong, the user easily inputs wrong or meaningless nucleic acid sequences, misoperation is generated, and the error rate is high. For designers with insufficient professional ability, analysis documents are consulted in a network library of < 32429in a popular way, an available sequence is found, which is difficult to add, the condition of incomplete consultation is likely to occur, time is wasted, and efficiency is low. The construction of the vector as a basic step of biological research is also a key step of experiment, and the method needs to be careful and cannot consume too much time, otherwise, the center of gravity of the experiment is shifted.
Disclosure of Invention
In view of this, the present invention provides a method for inserting a gene nucleic acid sequence, a computer storage medium and an electronic device, which can effectively reduce the mishandling rate of vector construction and improve efficiency.
In order to solve the above technical problems, in one aspect, the present invention provides a method for inserting a nucleic acid sequence of a gene, comprising the steps of: s1, constructing a nucleic acid sequence element library; s2, selecting a carrier framework, and arranging at least one insertion site on the carrier framework; s3, obtaining site information of an insertion site selected by a user, and calling a corresponding nucleic acid sequence in the nucleic acid sequence element library according to the site information; s4, obtaining the corresponding nucleic acid sequence from the nucleic acid sequence element library, and inserting the nucleic acid sequence into the insertion site corresponding to the site information; s5, editing the inserted nucleic acid sequence; s6, judging whether the nucleic acid sequence of the vector framework is in compliance, if so, generating the vector, and if not, sending a prompt message.
According to the method for inserting the gene nucleic acid sequence, the nucleic acid sequence element library is constructed in advance, when a user selects to insert a carrier element at a certain insertion site, a proper nucleic acid sequence can be called from the nucleic acid sequence element library, and the user only needs to select the proper nucleic acid sequence to add to the insertion site according to needs, so that the possibility of misoperation is greatly reduced, the misoperation rate is reduced, the construction efficiency is improved, and the method is more friendly to the user.
According to some embodiments of the invention, step S1 includes: s11, randomly obtaining a plurality of nucleic acid sequences and sequencing the nucleic acid sequences; s12, inserting the nucleic acid sequence passing the sequencing into a vector; s13, judging whether the nucleic acid sequence is effective through experiments; s14, selecting the effective nucleic acid sequence to construct the nucleic acid sequence element library.
According to some embodiments of the invention, in step S1, the libraries of nucleic acid sequence elements are classified according to their biological functions to obtain classification libraries, and the names, sequence lengths and basic information of the nucleic acid sequences in each classification library are labeled.
According to some embodiments of the present invention, in step S2, a plurality of fixed insertion sites are provided on each of the carrier backbones according to the function of the carrier to be constructed.
According to some embodiments of the present invention, in step S3, the locus information selected by the user is obtained according to the mouse event, and the corresponding classification library is called according to the locus information and displayed through a pop-up window.
According to some embodiments of the invention, in step S4, the corresponding nucleic acid sequences in the classification library are obtained according to mouse events.
According to some embodiments of the invention, in step S5, the user is restricted from entering non-biological coding characters during editing of the nucleic acid sequence.
According to some embodiments of the invention, step S5 further comprises: judging whether all the set insertion sites have inserted nucleic acid sequences, and executing step S6 after all the insertion sites have inserted nucleic acid sequences.
According to some embodiments of the invention, in step S6, the method for determining whether the nucleic acid sequence of the vector backbone is compliant is: judging whether the number of bases of the nucleic acid sequence is a multiple of 3; determining whether the nucleic acid sequence begins with a start codon; determining whether the nucleic acid sequence ends with a stop codon; determining whether the nucleic acid sequence uses an internal in-frame stop codon.
According to some embodiments of the invention, the prompt is to indicate the cause of the nucleic acid sequence violation or to ask the user whether to proceed.
In a second aspect, embodiments of the present invention provide a computer storage medium comprising one or more computer instructions that, when executed, implement a method as in the above embodiments.
An electronic device according to an embodiment of the third aspect of the invention comprises a memory for storing one or more computer instructions and a processor; the processor is configured to invoke and execute the one or more computer instructions to implement the method according to any of the embodiments described above.
Drawings
FIG. 1 is a flowchart of a method of inserting a nucleic acid sequence of a gene according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a vector backbone in a method of inserting a nucleic acid sequence of a gene according to an embodiment of the present invention;
FIG. 3 is a display of a popup window of a taxonomic library in the method of inserting a nucleic acid sequence of a gene according to an embodiment of the present invention;
fig. 4 is a schematic diagram of an electronic device according to an embodiment of the invention.
Reference numerals:
an electronic device 300;
a memory 310; an operating system 311; an application 312;
a processor 320; a network interface 330; an input device 340; a hard disk 350; a display device 360.
Detailed Description
The following detailed description of embodiments of the present invention will be made with reference to the accompanying drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The following first explains the related terms referred to in the present application.
Carrier: vector (Vector) refers to a self-replicating DNA molecule that transfers a DNA fragment (the gene of interest) to a recipient cell in a recombinant DNA technique. The three most commonly used vectors are bacterial plasmids, bacteriophages and animal and plant viruses. In actual life, insulin can be introduced into E.coli by using a vector into which a plasmid into which an insulin gene fragment has been inserted. The plasmid into which the gene fragment is inserted is called a vector. The plasmid can self-replicate in bacteria and does not affect the original activity of organisms.
Constructing a vector: vector construction is one of the commonly used means for molecular biology research. Mainly comprises the reconstruction of the multi-cloning site MCS of the existing vector and the reconstruction of functional elements such as the promoter, the enhancer, the screening marker and the like of the existing vector. The construction of the vector through a computer means that a new vector is constructed by inserting or modifying a nucleic acid sequence into a functional element needing to be modified in an existing vector framework.
Expression vector: expression vectors are basic operations of genetic engineering in biology, and expression vectors (expression vectors) are vectors which can express a target gene by adding expression elements (such as a promoter, an RBS (RBS), a terminator and the like) on the basis of a basic skeleton of a cloning vector. In the present application, an expression element is a vector element.
First, a method for inserting a nucleic acid sequence of a gene according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.
As shown in fig. 1, the method of inserting a nucleic acid sequence of a gene according to an embodiment of the present invention includes the steps of:
s1, constructing a nucleic acid sequence element library.
S2, selecting a carrier framework, and arranging at least one insertion site on the carrier framework.
S3, obtaining the site information of the insertion site selected by the user, and calling the corresponding nucleic acid sequence in the nucleic acid sequence element library according to the site information.
S4, obtaining the corresponding nucleic acid sequence from the nucleic acid sequence element library, and inserting the nucleic acid sequence into the insertion site corresponding to the site information.
S5, editing the inserted nucleic acid sequence.
S6, judging whether the nucleic acid sequence of the vector framework is in compliance, if so, generating the vector, and if not, sending a prompt message.
Therefore, according to the method for inserting the gene nucleic acid sequence, the nucleic acid sequence element library is constructed in advance, when a user selects to insert a vector element at a certain insertion site, a proper nucleic acid sequence can be called from the nucleic acid sequence element library, and the user only needs to select the proper nucleic acid sequence to add to the insertion site according to needs, so that the possibility of misoperation is greatly reduced, the misoperation rate is reduced, the construction efficiency is improved, and the method is more friendly to the user.
According to an embodiment of the present invention, step S1 includes:
s11, randomly obtaining a plurality of nucleic acid sequences and sequencing the nucleic acid sequences.
S12, inserting the nucleic acid sequence passing the sequencing into a vector, wherein the nucleic acid sequence passing the sequencing is a sequence with the recognized sequence corresponding to the theoretical sequence.
And S13, judging whether the nucleic acid sequence is effective or not through experiments.
S14, selecting the effective nucleic acid sequence to construct the nucleic acid sequence element library.
In other words, according to the method for inserting a gene nucleic acid sequence of the embodiment of the present invention, before inserting a nucleic acid sequence into a vector backbone, a nucleic acid sequence component library needs to be constructed, and the greatest difficulty in constructing the nucleic acid sequence component library is to search and analyze a large amount of biological literature to screen out an available nucleic acid sequence, perform sequencing, insert the nucleic acid sequence into a vector after the sequencing passes, and confirm the effectiveness of the fragment through several experiments to ensure the biological function. The process can be performed by those skilled in the art by reviewing the analysis literature, verifying the nucleic acid sequence, and packaging and classifying the nucleic acid sequence without having to spend time on the process by each user, thereby greatly saving time and effort of the user.
Preferably, in some embodiments of the present invention, in step S1, the libraries of nucleic acid sequence elements are classified according to their biological functions to obtain classification libraries, and the names, sequence lengths and basic information of the nucleic acid sequences in each classification library are labeled.
That is, after the nucleic acid sequence element library is constructed, the nucleic acid sequence name, the sequence length, the basic description, etc. can be packaged for convenient calling according to the collected effective information. Finally, nucleic acid sequences can be classified into the following 13 classes according to their biological function: promoter, ORF, Tag, Linker, shRNA Target Sequence, gRNA Target Sequence, minimum Promoter, Enhancer, Homology Arm, case, Regulatory Element.
Therefore, the nucleic acid sequence element libraries are classified to obtain a plurality of classification libraries, when a nucleic acid sequence is inserted into each insertion site, the corresponding classification libraries are called only according to the site information corresponding to the insertion site, the whole nucleic acid sequence element library is prevented from being called every time, the calling efficiency can be greatly improved, and the construction efficiency of the carrier is further improved.
As shown in fig. 2, according to an embodiment of the present invention, in step S2, a plurality of fixed insertion sites are provided on each of the carrier frameworks according to the functions of the carriers to be constructed.
Usually, the user selects the insertion site at will among the prior art, makes whole sequence confusion because of the point is wrong place easily, to this, this application sets up fixed insertion site for every carrier system according to the different functions of different carrier systems, has both guaranteed the flexibility of carrier design, can reduce the confusion again. The fixed insertion step also simplifies the carrier design process and facilitates operation.
Optionally, in some embodiments of the present invention, in step S3, the locus information selected by the user is obtained according to the mouse event, and the corresponding classification library is called according to the locus information and displayed through a pop-up window. Further, in step S4, the corresponding nucleic acid sequences in the classification library may also be obtained according to mouse events and then inserted into the vector backbone.
Specifically, as shown in fig. 3, according to the mouse event, the insertion site clicked by the user is obtained, and different classification libraries are called according to the type of the insertion site and displayed through a popup.
According to one embodiment of the invention, in step S5, the user is restricted from entering non-biological coding characters during editing of the nucleic acid sequence.
That is, after inserting the nucleic acid sequence at the insertion site, the user can edit the inserted element and limit the user's entry of non-biological coding characters (i.e., non-ATCG characters) during the editing process, thereby further reducing the error rate.
In some embodiments of the present invention, step S5 further includes: judging whether all the set insertion sites have inserted nucleic acid sequences, and executing step S6 after all the insertion sites have inserted nucleic acid sequences.
In other words, after editing the elements of each insertion site, the integrity of the vector can be further judged. Specifically, the existing carrier system technology cannot judge the type of the input sequence and also cannot judge the integrity of the carrier because a fixed insertion point is not set. In order to ensure the integrity of the vector in this application, all defined insertion sites must be inserted with elements. The specific operation can be as follows: after a user inserts an element at an insertion site, the flag parameter of the insertion site becomes True. After the flag parameter of all the insertion sites is changed to True, all the insertion sites are proved to be inserted.
According to one embodiment of the present invention, in step S6, the method for determining whether the nucleic acid sequence of the vector backbone is compliant is:
judging whether the number of bases of the nucleic acid sequence is a multiple of 3;
determining whether the nucleic acid sequence begins with a start codon;
determining whether the nucleic acid sequence ends with a stop codon;
determining whether the nucleic acid sequence uses an internal in-frame stop codon.
Further, the prompt message is to display the reason for the nucleic acid sequence violation or to ask the user whether to continue.
That is, this step can determine whether all vector sequences are compliant, if so, generate a vector, and if not, display the cause of the violation sequence and ask the user whether to continue.
Wherein, whether the vector sequence is substantially compliant can be judged according to the following conditions:
whether the number of bases is a multiple of 3, if so, then compliance is achieved, and if not, then compliance is not achieved;
whether to start with an initiation codon (ATG), if so, then compliance, and if not, then not compliance;
whether or not termination codon usage is terminated (TAA, TAG or TGA), if so, then compliance, and if not, then not compliance;
whether an internal in-frame stop codon (TAA, TAG or TGA) is used, if so, no compliance is made, if not, compliance is made.
In summary, the method for inserting a gene nucleic acid sequence according to the embodiment of the present invention is a method for dynamically inserting a verified nucleic acid sequence on a verified vector backbone, which is proposed to solve the problems of high error rate and low efficiency of the existing construction method. The method is characterized in that firstly, a verified meaningful nucleic acid sequence library is preset, and when a user singly inserts at an insertion point of a certain carrier element type, the system displays the nucleic acid sequence library of the type according to the type of the insertion point, and detailed description is provided for the user to select. The user finishes the addition of the nucleic acid sequence only by clicking a few buttons, so that the error rate of constructing the vector by a computing mechanism is greatly reduced, the efficiency is improved, and the method is more friendly to the user.
In addition, the present invention also provides a computer storage medium comprising one or more computer instructions that, when executed, perform any of the above-described methods for inserting a gene nucleic acid sequence.
That is, the computer storage medium stores a computer program that, when executed by a processor, causes the processor to perform any one of the above-described methods for inserting a nucleic acid sequence of a gene.
As shown in fig. 4, an embodiment of the present invention provides an electronic device 300, which includes a memory 310 and a processor 320, where the memory 310 is configured to store one or more computer instructions, and the processor 320 is configured to call and execute the one or more computer instructions, so as to implement any one of the methods described above.
That is, the electronic device 300 includes: a processor 320 and a memory 310, in which memory 310 computer program instructions are stored, wherein the computer program instructions, when executed by the processor, cause the processor 320 to perform any of the methods described above.
Further, as shown in fig. 4, the electronic device 300 further includes a network interface 330, an input device 340, a hard disk 350, and a display device 360.
The various interfaces and devices described above may be interconnected by a bus architecture. A bus architecture may be any architecture that may include any number of interconnected buses and bridges. Various circuits of one or more Central Processing Units (CPUs), represented in particular by processor 320, and one or more memories, represented by memory 310, are coupled together. The bus architecture may also connect various other circuits such as peripherals, voltage regulators, power management circuits, and the like. It will be appreciated that a bus architecture is used to enable communications among the components. The bus architecture includes a power bus, a control bus, and a status signal bus, in addition to a data bus, all of which are well known in the art and therefore will not be described in detail herein.
The network interface 330 may be connected to a network (e.g., the internet, a local area network, etc.), and may obtain relevant data from the network and store the relevant data in the hard disk 350.
The input device 340 may receive various commands input by an operator and send the commands to the processor 320 for execution. The input device 340 may include a keyboard or a pointing device (e.g., a mouse, a trackball, a touch pad, a touch screen, or the like).
The display device 360 may display the result of the instructions executed by the processor 320.
The memory 310 is used for storing programs and data necessary for operating the operating system, and data such as intermediate results in the calculation process of the processor 320.
It will be appreciated that memory 310 in embodiments of the invention may be either volatile memory or nonvolatile memory, or may include both volatile and nonvolatile memory. The nonvolatile memory may be a Read Only Memory (ROM), a Programmable Read Only Memory (PROM), an Erasable Programmable Read Only Memory (EPROM), an Electrically Erasable Programmable Read Only Memory (EEPROM), or a flash memory. Volatile memory can be Random Access Memory (RAM), which acts as external cache memory. The memory 310 of the apparatus and methods described herein is intended to comprise, without being limited to, these and any other suitable types of memory.
In some embodiments, memory 310 stores the following elements, executable modules or data structures, or a subset thereof, or an expanded set thereof: an operating system 311 and application programs 312.
The operating system 311 includes various system programs, such as a framework layer, a core library layer, a driver layer, and the like, and is used for implementing various basic services and processing hardware-based tasks. The application programs 312 include various application programs, such as a Browser (Browser), and are used for implementing various application services. A program implementing methods of embodiments of the present invention may be included in application 312.
The method disclosed by the above embodiment of the present invention can be applied to the processor 320, or implemented by the processor 320. Processor 320 may be an integrated circuit chip having signal processing capabilities. In implementation, the steps of the above method may be performed by integrated logic circuits of hardware or instructions in the form of software in the processor 320. The processor 320 may be a general purpose processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), an off-the-shelf programmable gate array (FPGA) or other programmable logic device, discrete gate or transistor logic, discrete hardware components, or any combination thereof, and may implement or perform the methods, steps, and logic blocks disclosed in the embodiments of the present invention. A general purpose processor may be a microprocessor or the processor may be any conventional processor or the like. The steps of the method disclosed in connection with the embodiments of the present invention may be directly implemented by a hardware decoding processor, or implemented by a combination of hardware and software modules in the decoding processor. The software module may be located in ram, flash memory, rom, prom, or eprom, registers, etc. storage media as is well known in the art. The storage medium is located in the memory 310, and the processor 320 reads the information in the memory 310 and completes the steps of the method in combination with the hardware.
It is to be understood that the embodiments described herein may be implemented in hardware, software, firmware, middleware, microcode, or any combination thereof. For a hardware implementation, the processing units may be implemented within one or more Application Specific Integrated Circuits (ASICs), Digital Signal Processors (DSPs), Digital Signal Processing Devices (DSPDs), Programmable Logic Devices (PLDs), Field Programmable Gate Arrays (FPGAs), general purpose processors, controllers, micro-controllers, microprocessors, other electronic units designed to perform the functions described herein, or a combination thereof.
For a software implementation, the techniques described herein may be implemented with modules (e.g., procedures, functions, and so on) that perform the functions described herein. The software codes may be stored in a memory and executed by a processor. The memory may be implemented within the processor or external to the processor.
In particular, the processor 320 is also configured to read the computer program and execute any of the methods described above.
In the several embodiments provided in the present application, it should be understood that the disclosed method and apparatus may be implemented in other ways. For example, the above-described apparatus embodiments are merely illustrative, and for example, the division of the units is only one logical division, and other divisions may be realized in practice, for example, a plurality of units or components may be combined or integrated into another system, or some features may be omitted, or not executed. In addition, the shown or discussed mutual coupling or direct coupling or communication connection may be an indirect coupling or communication connection through some interfaces, devices or units, and may be in an electrical, mechanical or other form.
In addition, functional units in the embodiments of the present invention may be integrated into one processing unit, or each unit may be physically included alone, or two or more units may be integrated into one unit. The integrated unit can be realized in a form of hardware, or in a form of hardware plus a software functional unit.
The integrated unit implemented in the form of a software functional unit may be stored in a computer readable storage medium. The software functional unit is stored in a storage medium and includes several instructions to enable a computer device (which may be a personal computer, a server, or a network device) to execute some steps of the transceiving method according to various embodiments of the present invention. And the aforementioned storage medium includes: various media capable of storing program codes, such as a usb disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk, or an optical disk.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (12)
1. A method for inserting a nucleic acid sequence of a gene comprising the steps of:
s1, constructing a nucleic acid sequence element library;
s2, selecting a carrier framework, and arranging at least one insertion site on the carrier framework;
s3, obtaining site information of an insertion site selected by a user, and calling a corresponding nucleic acid sequence in the nucleic acid sequence element library according to the site information;
s4, obtaining the corresponding nucleic acid sequence from the nucleic acid sequence element library, and inserting the nucleic acid sequence into the insertion site corresponding to the site information;
s5, editing the inserted nucleic acid sequence;
s6, judging whether the nucleic acid sequence of the vector framework is in compliance, if so, generating the vector, and if not, sending a prompt message.
2. The method according to claim 1, wherein step S1 includes:
s11, randomly obtaining a plurality of nucleic acid sequences and sequencing the nucleic acid sequences;
s12, inserting the nucleic acid sequence passing the sequencing into a vector;
s13, judging whether the nucleic acid sequence is effective through experiments;
s14, selecting the effective nucleic acid sequence to construct the nucleic acid sequence element library.
3. The method according to claim 1 or 2, wherein in step S1, the nucleic acid sequence element libraries are classified according to their biological functions to obtain classification libraries, and names, sequence lengths and basic information of the nucleic acid sequences in each classification library are labeled.
4. The method according to claim 3, wherein in step S2, a plurality of fixed insertion sites are provided on each of the carrier backbones according to the function of the carrier to be constructed.
5. The method according to claim 3, wherein in step S3, the locus information selected by the user is obtained according to the mouse event, and the corresponding classification library is called according to the locus information and displayed through a pop-up window.
6. The method according to claim 5, wherein in step S4, the corresponding nucleic acid sequences in the classification library are obtained according to mouse events.
7. The method of claim 1, wherein in step S5, the user is restricted from entering non-biological coding characters during editing of the nucleic acid sequence.
8. The method according to claim 1, wherein step S5 further comprises:
judging whether all the set insertion sites have inserted nucleic acid sequences, and executing step S6 after all the insertion sites have inserted nucleic acid sequences.
9. The method of claim 1, wherein in step S6, the method for determining whether the nucleic acid sequence of the vector backbone is compliant is:
judging whether the number of bases of the nucleic acid sequence is a multiple of 3;
determining whether the nucleic acid sequence begins with a start codon;
determining whether the nucleic acid sequence ends with a stop codon;
determining whether the nucleic acid sequence uses an internal in-frame stop codon.
10. The method of claim 1 or 9, wherein the prompt is to display the cause of the nucleic acid sequence violation or to ask the user whether to proceed.
11. A computer storage medium comprising one or more computer instructions which, when executed, implement the method of any one of claims 1-10.
12. An electronic device comprising a memory and a processor, wherein,
the memory is to store one or more computer instructions;
the processor is configured to invoke and execute the one or more computer instructions to implement the method of any one of claims 1-10.
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| CN112687338A (en) * | 2020-12-31 | 2021-04-20 | 云舟生物科技（广州）有限公司 | Method for storing and restoring gene sequence, computer storage medium and electronic device |
| CN115881225A (en) * | 2022-12-28 | 2023-03-31 | 云舟生物科技(广州)股份有限公司 | Method for analyzing biological information sequence, computer storage medium, and electronic device |
| CN115982034A (en) * | 2022-12-30 | 2023-04-18 | 云舟生物科技(广州)股份有限公司 | Test method of carrier construction system virtual terminal, storage medium and electronic equipment |
| CN117789825A (en) * | 2023-12-29 | 2024-03-29 | 云舟生物科技(广州)股份有限公司 | Gene source CDS sequence editing recording method, computer storage medium and electronic equipment |
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| CN112687338B (en) * | 2020-12-31 | 2022-01-11 | 云舟生物科技（广州）有限公司 | Method for storing and restoring gene sequence, computer storage medium and electronic device |
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