CN109102369A

CN109102369A - It is constructed on a kind of carrier line and method, system, platform and the storage medium of transaction

Info

Publication number: CN109102369A
Application number: CN201810929034.7A
Authority: CN
Inventors: 蓝田; 李钟文; 蒙伟能; 杨金邦
Original assignee: Yun Zhou Biotechnology (guangzhou) Co Ltd
Current assignee: Yun Zhou Biotechnology (guangzhou) Co Ltd
Priority date: 2018-08-15
Filing date: 2018-08-15
Publication date: 2018-12-28

Abstract

The present invention relates to biological information fields, and in particular to constructs on a kind of carrier line and method, system, platform and the storage medium of transaction.The present invention passes through acquisition demand carrier data；Carry out data selection and first time building processing；Editor and second of building processing are carried out as needed；Then filtering detection；It will test qualified demand carrier to be synthesized, ultimately generate the demand carrier that building is completed.It obtains and detects demand carrier data that is qualified and having synthesized and carry out valuation measurement；Generate corresponding price list；Confirm that data generate building and delivery status information trace list under demand carrier line according to price list, is finally completed online trading.The policy specification and standardization that genophore can be made to design, and building process is simple, substantially reduces construction schedule and improves production efficiency, while the efficient customization of biotechnology service product also may be implemented, saves a large amount of manpower and material resources.The building and transaction flow for having standardized itself are allowed to more rationally and efficient.

Description

It is constructed on a kind of carrier line and method, system, platform and the storage medium of transaction

Technical field

The present invention relates to biological information fields, and in particular to constructs on a kind of carrier line and the method for transaction, system, platform And storage medium.

Background technique

As human genome is gradually decrypted, genophore has become gene functional research, medicament research and development, in-vitro diagnosis With one of the important research tool in personalized treatment.After molecule clone technology is born in considerably long one of the time, gene is carried The construction work of body is all that laboratory researcher voluntarily undertakes.Demander is designer, and is the producer.Due to gene Carrier system multiplicity, carrier framework it is multifarious, experimenter have to take a substantial amount of time go to consult with energy it is numerous Document designs and constructs the genophore for meeting experiment needs, however, such investment is often also difficult to reach expected effect Fruit.

Firstly, the strategy of tradition genophore design is very mixed and disorderly at present, does not have standard.Because without criterion how Design can just make carrier bring about the desired effect, so designer is often to indiscriminately imitate the way of forefathers to go to realize, and forefathers set Meter set pattern is set up based on scientific and technological level at that time, so usually being difficult to meet the research under existing scientific and technological level again Demand.

Secondly, the building process of genophore is extremely complex.It since demander is designer, and is the producer, not The reasonable division of labor is occurred, thus causes production efficiency low.

In recent years, occur some third party's service outsourcings company in the market and genophore building service is provided.But it is basic It is all to continue to use traditional laboratory handicraft workshop mode, standard is different, level is different, and industrialization degree is low, leads to structure Build that the period is very long, the carrier of delivery sometimes can not achieve the effect etc. of expectations of customer.

Summary of the invention

For tactful mixed and disorderly, complicated, designer and the production without standard and building process of above genophore design The problems such as person's division of labor is unreasonable, horizontal different, very long low with production efficiency so as to cause construction schedule, the present invention provides one kind The efficient fixed of biotechnology service product may be implemented in method, system, platform and the storage medium for constructing and trading on carrier line System, saves a large amount of manpower and material resources of user voluntarily DIY, but also has standardized itself production and transaction flow, is allowed to more Rationally and efficiently.

The present invention is realized especially by following technical scheme:

A kind of method constructed on carrier line, the method specifically comprise the following steps:

Step S10 obtains demand carrier data；

Step S20, carrier data, carries out corresponding demand carrier data selection and first time building processing according to demand；

Step S30 handles chosen and building for the first time treated demand carrier carries out editor and second of building；

Step S40, the demand carrier completed to building processing are filtered detection；

Step S50 will test qualified demand carrier and be synthesized, and ultimately generate the demand carrier that building is completed.

Further, the demand carrier data includes demand carrier type data information and demand carrier framework data letter Breath；

It is described to carry out corresponding demand carrier data selection and first time building processing, specifically: to the selection of list, to base Because of the search and insertion of information, to the search and insertion of sequence data；

To chosen and building for the first time treated demand carrier carries out editor and second of building processing, specifically: It adds sequence label and sequence is edited or is modified.

Further, further include following steps before step S10:

Step S00 receives list and transmits data；

List transmission data are saved into database, and carried out at the judgement of scheme demand for form data by step S01 Reason；

Step S02, by determination processing result data real-time informing vector construction demander.

Further, list transmission data include selected service type data, the text data of input and optional Upload file data；

The determination processing includes the artificial customer service processing of the processing of system intelligent decision and backstage；

The real-time informing includes online real-time informing and mail notification.

Further, further include following steps among step S20:

Step S21 obtains demand carrier system data；

Step S22, carrier system data, search for required gene data and sequence data according to demand；

Step S23 carries out selection or insertion process to demand carrier in conjunction with the gene data and sequence data searched；

Step S24 determines chosen and building for the first time treated whether demand carrier carry out editor and second Secondary building processing；It is no to then follow the steps S40 if so then execute step S30.

Further, further include following steps among step S30:

Step S31 obtains the demand carrier data for carrying out editor and second of building processing；

Step S32 is edited or is modified to demand carrier data by corresponding functionality controls；

Step S33 shows editor or the demand carrier data that modification is completed.

Further, further include following steps among step S40:

Whether step S41, the demand carrier sense for determining that building processing is completed are qualified；If qualification thens follow the steps S50, no Then follow the steps S20.

In order to achieve the above objectives, the present invention also provides a kind of method of carrier online trading, the method is specifically included Following steps:

Step S100 obtains the demand carrier data that detection is qualified and has synthesized；

Step S200 carries out valuation measurement according to the type detail for the demand carrier that detection is qualified and has synthesized；

Step S300 generates the price list of corresponding demand carrier data；

Step S400 generates building and delivery status letter under demand carrier line according to the confirmation data that quotation payment is completed Breath tracking is single, is finally completed demand carrier online trading；

The valuation measurement includes the intelligently valuation measurement of manual invoicing measurement and system.

Further, further include following steps among step S300:

Step S301 shows corresponding demand carrier price data, and real-time core is to demand carrier data price list；

Step S302 obtains approved quotation forms data, generates and detects demand carrier data that is qualified and having synthesized Downloading data；

Quotation payment completion confirmation data are sent under corresponding demand carrier line and construct department by step S303；

Further, described to show that corresponding demand carrier price data includes showing interface demand carrier price data and postal Part mode reminds the price data of demand carrier in real time.

The real-time core includes: to carry out the approval of quotation detail and reality to demand carrier in real time to demand carrier data price list When whether the quotation of demand carrier is completed by payment is checked and approved；

If the quotation of demand carrier has been paid, S302 is thened follow the steps, otherwise step S301.

In order to achieve the above objectives, the present invention also provides the system constructed on a kind of carrier line, the system includes:

First acquisition unit, for obtaining demand carrier data；

First building processing unit carries out corresponding demand carrier data selection and first for carrier data according to demand Secondary building processing；

Second building processing unit, for chosen and building for the first time treated demand carrier carries out editor and the Secondary building processing；

Detection unit is filtered, the demand carrier for completing to building processing is filtered detection；

Synthesis unit, the demand carrier for will test qualified are synthesized, and the demand carrier that building is completed is ultimately generated.

Further, the system further include:

First obtains module, for receiving list transmission data；

First determination module for saving list transmission data into database, and carries out scheme for form data Demand determination processing；

Notification module is used for determination processing result data, real-time informing vector construction demander.

Further, the first building processing unit includes:

Second obtains module, for obtaining demand carrier system data；

Search module searches for required gene data and sequence data for carrier system data according to demand；

Insertion process module, for combining the gene data and sequence data that search, to demand carrier carry out selection or Insertion process；

Second determination module, for determining chosen and building for the first time, treated whether demand carrier is compiled It collects and second of building is handled.

Further, the second building processing unit includes:

Third obtains module, for obtaining the demand carrier data for carrying out editor and second of building processing；

Edit-modify module, for by corresponding functionality controls, demand carrier data being edited or being modified；

First display module, the demand carrier data completed for showing editor or modification.

Further, the filtering detection unit includes:

Whether third determination module, the demand carrier sense for determining that building processing is completed are qualified.

In order to achieve the above objectives, the present invention also provides a kind of system of carrier online trading, the system includes:

Second acquisition unit, for obtaining the demand carrier data that detection is qualified and has synthesized；

Measuring unit of valuating carries out valuation survey for the type detail according to the demand carrier that detection is qualified and has synthesized Amount；

Quotation generation unit, for generating the price list of corresponding demand carrier data；

State tracking module is constructed and is handed under demand carrier line for generating according to the confirmation data that quotation payment is completed It is single to pay status information tracking, is finally completed demand carrier online trading.

Further, the system also includes:

It shows and checks and approves module, for showing corresponding demand carrier price data, and real-time core offers to demand carrier data It is single；

Generation module is downloaded, for obtaining approved quotation forms data, generates the demand load that detection is qualified and has synthesized The downloading data of volume data；

Confirm sending module, is sent under corresponding demand carrier line and constructs for the payment completion confirmation data that will offer Department.

In order to achieve the above objectives, the present invention also provides the platforms for constructing and trading on a kind of carrier line, comprising:

The platform courses program for constructing and trading on processor, memory and carrier line；

Wherein the platform courses program, the platform courses for constructing and trading on the carrier line are executed in the processor Program is stored in the memory, and the platform courses program for constructing and trading on the carrier line realizes the carrier The method and step of the method and step and the carrier online trading that are constructed on line.

In order to achieve the above objectives, the present invention also provides a kind of computer-readable storage mediums, described computer-readable Storage medium is stored with the platform courses program for constructing and trading on carrier line, the platform control for constructing and trading on the carrier line Processing procedure sequence realizes the method and step of the method and step constructed and the carrier online trading on the carrier line.

Compared with prior art, the invention has the following advantages:

The present invention realizes the method constructed on carrier line by a kind of, the specific steps are as follows:

Step S10 obtains demand carrier data；

And correspondingly system unit:

First acquisition unit, for obtaining demand carrier data；

Pass through a kind of method for realizing carrier online trading, the specific steps are as follows:

Step S300 generates the price list of corresponding demand carrier data；

Step S400 generates building and delivery status letter under demand carrier line according to the confirmation data that quotation payment is completed Breath tracking is single, is finally completed demand carrier online trading.

And correspondingly system unit:

Status tracking unit is constructed and is handed under demand carrier line for generating according to the confirmation data that quotation payment is completed It is single to pay status information tracking, is finally completed demand carrier online trading.

The policy specification and standardization that genophore can be made to design, and building process is simple, designer and Sheng Production person's rational division of labor to substantially reduce construction schedule and improve production efficiency, while also achieving biotechnology service production The efficient customization of product saves a large amount of manpower and material resources of user voluntarily DIY.This logical process also standardized itself production and Transaction flow is allowed to more rationally and efficient.

Detailed description of the invention

To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment Attached drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the invention, for For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other Attached drawing.

Fig. 1 is the method framework flow diagram constructed on a kind of carrier line of the present invention；

Fig. 2 is the building operational architecture flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 3 is the second preferred embodiment framework flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 4 is the requirement validation framework flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 5 is the third preferred embodiment framework flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 6 is the search framework flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 7 is the 4th preferred embodiment framework flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 8 is the 5th preferred embodiment framework flow diagram of the method constructed on a kind of carrier line of the present invention；

Fig. 9 is a kind of method framework flow diagram of carrier online trading of the present invention；

Figure 10 is a kind of method quotation framework flow diagram of carrier online trading of the present invention；

Figure 11 is a kind of method transaction payment framework flow diagram of carrier online trading of the present invention；

Figure 12 is a kind of 6th preferred embodiment framework flow diagram of the method for carrier online trading of the present invention；

Figure 13 is the system architecture schematic diagram constructed on a kind of carrier line of the present invention；

Figure 14 is the module architectures schematic diagram of the system constructed on a kind of carrier line of the present invention；

Figure 15 is a kind of system architecture schematic diagram of carrier online trading of the present invention；

Figure 16 is a kind of module architectures schematic diagram of the system of carrier online trading of the present invention；

Figure 17 is the framework for the terminal that the method and system embodiment for constructing and trading on a kind of carrier line of the present invention provides Schematic diagram；

Figure 18 is computer-readable storage medium structural schematic diagram in an embodiment of the present invention；

Description of symbols:

3- terminal；31- memory；32- processor；33- communication bus；34- shows screen；

The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.

Specific embodiment

Purposes, technical schemes and advantages to facilitate the understanding of the present invention are clearer, with reference to the accompanying drawing and have The invention will be further described for the embodiment of body, and those skilled in the art can be by content disclosed in the present specification easily Understand further advantage and effect of the invention.

The present invention also can be implemented or be applied by other different specific examples, and the various details in this specification is also Various modifications and change can be carried out without departing from the spirit of the present invention based on different viewpoints and application.

It is to be appreciated that if relating to directionality instruction (such as up, down, left, right, before and after ...) in the embodiment of the present invention, Then directionality instruction be only used for explain under a certain particular pose (as shown in the picture) between each component relative positional relationship, Motion conditions etc., if the particular pose changes, directionality instruction is also correspondingly changed correspondingly.

In addition, being somebody's turn to do " first ", " second " etc. if relating to the description of " first ", " second " etc. in the embodiment of the present invention Description be used for description purposes only, be not understood to indicate or imply its relative importance or implicitly indicate indicated skill The quantity of art feature." first " is defined as a result, the feature of " second " can explicitly or implicitly include at least one spy Sign.It secondly, the technical solution between each embodiment can be combined with each other, but must be with those of ordinary skill in the art's energy Based on enough realizations, when the combination of technical solution appearance is conflicting or cannot achieve, it will be understood that this technical solution In conjunction with being not present, also not the present invention claims protection scope within.

Preferably, building and method of commerce are applied in one or more terminal or server on carrier line of the invention In.The terminal be it is a kind of can according to the instruction for being previously set or store, automatic progress numerical value calculating and/or information processing Equipment, hardware include but is not limited to microprocessor, specific integrated circuit (ApplicationSpecificIntegratedCi Rcuit, ASIC), programmable gate array (Field-ProgrammableGateArray, FPGA), digital processing unit (DigitalSignalProcessor, DSP), embedded device etc..

The terminal can be desktop PC, notebook, palm PC and cloud server etc. and calculate equipment.It is described Terminal can carry out human-computer interaction by modes such as keyboard, mouse, remote controler, touch tablet or voice-operated devices with client.

The present invention is to realize to construct and trade on carrier line, the method and system constructed on a kind of carrier line provided.

It as shown in Fig. 1 and Fig. 9, is the flow chart and one kind of construction method on a kind of carrier line provided in an embodiment of the present invention The flow chart of carrier online trading method.

In the present embodiment, the method for constructing and trading on the carrier line, can be applied to the end for having display function In end or fixed terminal, the terminal is not limited to PC, smart phone, tablet computer, is equipped with camera Desktop computer or all-in-one machine etc..

The method for constructing and trading on the carrier line also can be applied to by terminal and by network and the terminal into In the hardware environment that the server of row connection is constituted.Network includes but is not limited to: wide area network, Metropolitan Area Network (MAN) or local area network.This hair The method for constructing and trading on the carrier line of bright embodiment can be executed by server, can also be executed by terminal, may be used also Being executed jointly by server and terminal.

For example, can directly integrate the present invention at the terminal for the terminal for needing to carry out to construct and trade on carrier line Method provided by construct on carrier line and trading function, or installation is for realizing the client of method of the invention.Again Such as, method provided by the present invention can be transported in the form of Software Development Kit (SoftwareDevelopmentKit, SDK) Row provides the interface of building and trading function on carrier line, terminal or other equipment in the equipment such as server in the form of SDK The function of constructing and trade on intelligent carrier line can be realized by provided interface.

As shown in Figure 1, the present invention provides a kind of method constructed on carrier line, the flow chart according to different requirements, The sequence of middle step can change, and certain steps can be omitted.The method specifically comprises the following steps:

Step S10 obtains demand carrier data；

The demand carrier data includes demand carrier type data information and demand carrier framework data information；

Specifically, as shown in Fig. 2, demand carrier data is categorized into carrier multiple simple according to carrier type and skeleton Clear carrier system illustrates that user can be convenient chooses oneself desired carrier according to the guidance of each carrier system System.After selecting carrier system, into carrier design interface.Carrier design interface has used the mode of motion graphics interaction, Vector map and other information are enumerated.

In the carrier system selection page, lists according to the arrangement of certain logic level and designed on current system Support Line Carrier system type and technology guide document.Carrier system can be associated with the mark such as carrier type and skeleton, select carrier system After system, backstage is sent instructions to, rear end returns to the design page, and (the vector construction page, is related to carrier design all below i.e. on line Refer to vector construction).

In the carrier design page, contain carrier system title, design carrier annular can interaction figure, insertion can be sheared Element number, component type and its insertion position, the attribute of each class component commonly uses and components database and element recommended to close Keyword search, input personalization sequence construct personalisation elements, and element editor waits elements and function.In the process, it needs pair The element of insertion in need carry out insertion operation (be to demand carrier synthesize), the sequence of insertion element, complete on skeleton At the design (i.e. vector construction on line) of carrier.When designing completion, it is sent completely instruction, complete design instruction can will design number According to backstage is sent to, instruction and data is finished receiving from the background, it will the data for merging the design page, subsequently into filter, Part does not meet the design data of the type conventional design, can obtain pop-up prompt, informs front end, the data of this secondary design may It is not scientific, after front end sends and cancels instruction, the design page is returned, data before all retain, if front end selection continues, Design result will be obtained, carrier results page is jumped to.

That is, client's vector construction on line, it is only necessary to by a few step simple selections and click, carrier can be completed Design, obtain objective result.More more individual character can be obtained by searching for or voluntarily inputting and edit personal sequence The carrier and design result of change.This design as a result, containing the various biological informations of customer demand, client can choose Downloading vector reporter is more researched and analysed to do.

Preferably, the present embodiment applies also for the method for being inserted into carrier framework after pre-processing to shRNA sequence fragment；It is right The method of carrier framework is inserted into after the pretreatment of gRNA sequence；To the method for being inserted into carrier framework after the pretreatment of multiple ORF sequences； The judgment method of the biological effectiveness after carrier framework is inserted into complex sequence segment；According to user's sequence fragment dynamic generation The method of Vector map；Method (the Restriction enzyme site of restriction enzyme site analysis is carried out to carrier data online analysis)；The editor of the Protein tag of sequence fragment, online sequence fragment are searched and are marked online ORF and to sequence The name of segment.

As shown in figure 3, be second preferred embodiment of the invention, a kind of method constructed on carrier line, in step S10 it Before, further include following steps:

Step S00 receives list and transmits data；

The list transmission data include selected service type data, the text data of input and optional upload number of files According to；

Specifically, as shown in figure 4, client be unfamiliar with operation of the present invention step and tool using or there are any other In the case where demand query, transmission bearer requirement can choose.By simply selecting and input data, it is sent to rear end, after End possesses powerful technical support team, helps Customer design and returns to design scheme.

In the embodiment of the present invention, receives list transmission data and save in the database.The data of transmission mainly include three A part, first be selection service type data, second be input text data, third is optional upper transmitting file Data.

That is, submitting data each time, it can save recorded as one in the database.This data records exhibitions Show in the consistency operation page.The demand data submitted by reading analysis returns to answer or demand is directly added in return Design result.

It returns to answer or as a result, can notify submission side by one or two kinds of modes.One is the information sides in station Formula can check all demands and reply in " my account " column after logging in using account number.Another is to send postal Part notice.

In the embodiment of the present invention, the proposition of a demand, may be related to it is multiple recycle back and forth, can just obtain last solution Certainly.

Specifically, second preferred embodiment of the invention specifically comprises the following steps:

Step S00 receives list and transmits data；

Step S10 obtains demand carrier data；

Step detail is being described above, and details are not described herein again.

As shown in figure 5, be third preferred embodiment of the invention, a kind of method constructed on carrier line, in step S20 it In, further include following steps:

Step S21 obtains demand carrier system data；

Step S24 determines chosen and building for the first time treated whether demand carrier carry out editor and second Secondary building processing；

It is no to then follow the steps S40 if so then execute step S30.

Specifically, in third preferred embodiment of the invention, carrier system data according to demand are mainly concerned with, search must The gene data and sequence data needed, as shown in fig. 6, establishing, based on VB, (i.e. VectorBuilder is that company is exclusively used in carrying Body construct provided by a design platform) database and PostgreSQL database (NCBI) search engine.User's (i.e. bearer requirement Person) can be convenient by selection species and input keyword search to oneself desired gene data information and sequence number it is believed that Breath.Search module has used the mode of two keywords, and a keyword (species) is selection, another keyword is input Character string.The keyword of input can be following several:

Gene Name (such as: RHO (rhodopsin gene)), gene full name (such as: rhodopsin (rhodopsin base Cause)), other titles (such as: rod pigment (rod pigment)), NCBI gene I/D (such as: 6010), mRNA RefSeq accession (mRNA reference sequences number, such as: NM_000539.3), Protein RefSeq accession (protein reference sequence number, such as: NP_000530.1).

That is, one species of selection, or do not select, it is all species of default.

In embodiments of the present invention, search key is inputted, inquiry is clicked and data is submitted to send search instruction, will be searched Hitch fruit returns.If searched as a result, three column can be divided to show that far Left row is to indicate species, there is picture and text two Kind information is shown simultaneously.Middle column be essential information, such as title, full name, alias, other titles, chromosome until, gene class Type and NCBI gene I/D.Right-hand column is transcript (transcript) number for showing gene, and check the operation of sequence by Key.

Sequence key is checked in click, then can send instructions to gene pool, pulls details, popup web page.Meeting in the page Show that RefSeq (reference sequences) transcript may the also information of cloned template in stock.Wherein RefSeq (reference sequences) is transcribed This, can list Transcript ID (transcript ID), ProteinID (protein ID), there are also sequences.Wherein have editor's base The function button of cause, click, which will will pop up, jumps to sequence editor module.It is searched if it is during carrier construction using gene Rope module, there will be the keys being added to this gene in carrier here.

It also relates to, in conjunction with the gene data and sequence data searched, selection or insertion process is carried out to demand carrier.

Specifically, in embodiments of the present invention, demand carrier data is categorized into carrier more according to carrier type and skeleton A simple and clear carrier system illustrates that it is desired that user can be convenient chooses oneself according to the guidance of each carrier system Carrier system.After selecting carrier system, into carrier design interface.Carrier design interface has used motion graphics interaction Mode, Vector map and other information are enumerated.

In the carrier design page, contain carrier system title, design carrier annular can interaction figure, insertion can be sheared Element number, component type and its insertion position, the attribute of each class component commonly uses and components database and element recommended to close Keyword search, input personalization sequence construct personalisation elements, and element editor waits elements and function.In the process, it needs pair Insertion in need element carry out insertion operation, the design (i.e. vector construction on line) of carrier is completed, as to demand carrier Carry out selection or insertion process.

Preferably, in embodiments of the present invention, there is also determine chosen and building for the first time treated demand carrier Whether editor and second building processing are carried out；

That is, if so then execute step S30: to chosen and building for the first time, treated that demand carrier is compiled It collects and second of building is handled；No to then follow the steps S40: the demand carrier completed to building processing is filtered detection.

Specifically, third preferred embodiment of the invention specifically comprises the following steps:

Step S10 obtains demand carrier data；

Step S21 obtains demand carrier system data；

It is no to then follow the steps S40 if so then execute step S30.

Step S20 as of the invention, carrier data, carries out corresponding demand carrier data selection and first time according to demand Building processing；

Preferably, the present embodiment applies also for the method for being inserted into carrier framework after pre-processing to shRNA sequence fragment；It is right The method of carrier framework is inserted into after the pretreatment of gRNA sequence；To the method for being inserted into carrier framework after the pretreatment of multiple ORF sequences； The judgment method of the biological effectiveness after carrier framework is inserted into complex sequence segment；According to user's sequence fragment dynamic generation The method of Vector map；Method (the Restriction enzyme site of restriction enzyme site analysis is carried out to carrier data online analysis)；The editor of the Protein tag (protein tag) of sequence fragment, online sequence fragment are searched and marked online ORF and name to sequence fragment.

As shown in fig. 7, be four preferred embodiment of the invention, a kind of method constructed on carrier line, in step S30 it In, further include following steps:

Specifically, the translation that addition and sequence of the editor including label are carried out to demand carrier；In daily, on line The sequence edit tool for not being weaker than client installation version, bearer requirement person can open and edit anywhere or anytime oneself demand The corresponding sequence of carrier, and its editor for more meeting biology user and use habit.Have in VectorBuilder platform more The different classes of common component of kind, can construct the different sequence editting function module of included function, be generally a standard Sequence editor module, title, description, the sequence, the sequence calculated automatically, residue of top meeting flag sequence.Wherein sequence It is an editable text column, base sequence therein is to be divided into eight column groups for one group with 10 bases, arranged Characters show.

Preferably, arbitrarily can add and delete in edit box series (ATCG), editing machine can mark addition The sequence of sequence and deletion.The sequence wherein added, the sequence that can be identified in the position of addition, and deleted with green, can be on the right side Side information column show deletion position and deleted base sequence.

Sequence editor increases some conveniently label and functional modules:

It adds label: when the sequence of editor is element ORF, having the function of optional addition sequence label.It clicks List optional Accel label later, for example there are five the labels of classification: protein tag, purification tag, proteolytic cleavage site, Signal peptide and detection label.Tag names are chosen, may be selected sequence label being added to C-terminal or N-terminal.After selection determines, meeting Sequence label is inserted into element sequences.

Interpretative function: element sequences are translated into protein sequence.

It finds ORF: sequence being patterned linear displaying, wherein marking initiation codon with green P character (ATG), terminator codon (TAA, TAG, TGA) is marked with red vertical line.It is originated respectively from first base, second base Starting and the starting of third base, list three line marker graphics.When cursor being placed in reading frame above, can show Shortest reading frame around cursor.If clicking this reading frame, the corresponding sequence in edit box will be selected, check reverse complemental sequence Column, finally obtain the reverse complementary sequence of sequence.

In embodiments of the present invention, demand carrier data is shown, that is to say, that carrier is listed by ad hoc fashion typesetting Details contain the column of summary, map, element, sequence and price etc..Bearer requirement person very clear can be closed Key information.

Specifically, in carrier results page.Output illustrates the result data of a design vector, contains essential information Show that column, ordering information show that column, Vector map show that column, carrier sequence show that column and carrier element show version Block.

Essential information shows column, and number, title, type and all kinds of insertion elements of display carrier, there are also carriers Plasmid copy number, resistance etc..

Order information show column, display carrier building service delivery of items, price, the period, downstream value-added service with And the addition shopping cart function of carrier and service；Its middle and lower reaches value-added service is when design vector generates, according to carrier Type and feature, while what is generated corresponds to the carrier or relevant downstream service, services as plasmid extracts, virus packaging clothes Business etc., control plasmid, control virus etc..

Vector map shows column, then is typical genophore circular chart, the ID for identifying carrier simplified, name, The information such as sequence length element title and position length.Wherein different elements are identified with different colors.It additionally provides Selection area on map, scaling, the function of downloading.

Carrier sequence shows column, then is that the complete sequence of carrier is shown, wherein can be with different fonts and underscore face Color identifies the sequence of carrier element.

Carrier element shows column, is the component attributes of display carrier, including title, starting and final position, and size is (long Degree), type, description and application note etc., also check that the selection of more detailed information determines function.Wherein, this secondary design is inserted into Carrier, can be identified in name column with red.

In all displaying columns of top, Vector map shows that column, carrier sequence show column and carrier element exhibition Show column, these three show that column is interdependence interaction.Show that any displaying column on column carries out a little at three It hits and chooses, can all be shown at other two and interact the identical point of display in column.

Specifically, four preferred embodiment of the invention specifically comprises the following steps:

Step S10 obtains demand carrier data；

Step S30 as of the invention, to chosen and building for the first time treated demand carrier carries out editor and the Secondary building processing；

As shown in figure 8, be fifth preferred embodiment of the invention, a kind of method constructed on carrier line, in step S40 it In, further include following steps:

Specifically, it obtains and constructs the demand carrier data that processing is completed, the detection instrument in tool storage room in calling system, Whether the demand carrier sense for determining that building processing is completed by the detection instrument of selection is qualified, if qualification thens follow the steps S50:, it will test qualified demand carrier and synthesized, ultimately generate the demand carrier that building is completed；It is no to then follow the steps S20: Carrier data according to demand carries out corresponding demand carrier data selection and first time building processing.

That is, building is handled completion instruction and corresponding data are sent when the processing of demand vector construction is completed To backstage, instruction and data is finished receiving from the background, it will the data for merging the design page detect program subsequently into filtering, if If part does not meet the design data of the type conventional design, pop-up prompt can be obtained, informs front end or bearer requirement person, this The data of design may not be scientific, and after front end sends and cancels instruction, system will return to the design page, and data before are whole Retain, if front end selection continues, design result will be obtained, jump to carrier results page.

Detection instrument in the tool storage room, including being related to all tools to carrier sense, such as: alignment's work Tool, fixing nucleic acid dot matrix figure tool, shRNA target site design tool, codon optimization tool, G/C content calculating instrument, DNA second level knot Structure tool etc..

Bioscience research and experiment, the data being related to are extremely complex, but also there are intensive, some computers Tool software, can great support study dies personnel, save time and efforts.VB in itself experiment and R&D process by summarizing Experience, develop a series of biology tool.Preferably, the client for not needing to download and install a huge valuableness is soft Part can directly open browser, so that it may easily use.

At present in tool storage room, six kinds of LnkTools are shared using tool: alignment's tool, fixing nucleic acid dot matrix figure tool, ShRNA target site design tool, codon optimization tool, G/C content calculating instrument and DNA secondary structure tool.

Alignment's tool is compared to two sections of sequences, and there are three types of compare type: being specially DNA comparison, protein The DNA of comparison and protein sequence is compared.Input needs the sequence compared respectively in two input frames, then two sequences It is respectively labeled as sequence " 1 " and sequence " 2 ".Selection submission sends data to server and compares operation, then acquisition pair It is returned than result.Contrast model (type) can be shown in results page, gap weight, extend weight, score weight, sequence " 1 " Length, sequence " 2 " length, alignment length, same sex rate, similar sex rate and interval rate.

Fixing nucleic acid dot matrix figure tool is to calculate and indicate two sections of sequencing nucleic acid points with dot matrix graphic.Optional sequence type has DNA and two kinds of RNA.Two sections of sequences, Selective type, with default value are inputted respectively in two input frames of sequence " 1 " and sequence " 2 " There are two types of optional settings, and one is the setting of the size of sliding window, default values 10；Another kind is the base for allowing mispairing Number, default value is 0.It submits available dot matrix diagram data after data to return, jumps to dot matrix diagram page.In results page, use Two right angle orientations of two-phase rectangular plots identify the length of two sections of sequences, intermediate to list two sections with red and green respectively The fixing nucleic acid dot matrix figure of the forward and reverse complementary series of sequence.

ShRNA target site design tool is the sequence according to one section comprising shRNA, calculates target sequence.That is, One section of sequence comprising shRNA of input, then submits and is returned the result.Result may be one or more target site sequence Column and its initial position in the sequence, score value.

Codon optimization tool is to carry out measuring and calculating optimization to stream cipher.That is, one section of sequence of input, selection The type of sequence.Here the type that can choose has DNA/RNA sequence or two kinds of protein sequence.Also need to select the object of sequence Kind (such as: the mankind, mouse).It in addition is the restriction enzyme point for needing to evade there are one optional attribute.Data are submitted To after server, it will obtain result and return.As a result the sequence of paste is illustrated in, sequence and sequence difference after optimization Relative adaptability two-dimensional coordinate figure curve.

G/C content calculating instrument is the percentage composition of base in the sequence of calculation.In addition to the sequence of input itself, there are two Optional setting with default value.One is the setting of stroke window size, and default value is 30, and one is the setting of base type, is write from memory Recognizing value is GC.Wherein base type only receives standard base and represents letter five, ATCGU.After submitting data, result can be obtained The page returns.It is illustrated with two-dimensional coordinate figure curve as a result, and being labelled with sequence overall length, the percentage composition of every kind of base.

DNA secondary structure tool is exactly the secondary structure that graphics mode shows DNA.List entries is submitted to backstage, returns Return figure.

Specifically, fifth preferred embodiment of the invention specifically comprises the following steps:

Step S10 obtains demand carrier data；

Step S40 as of the invention, the demand carrier completed to building processing are filtered detection；

Preferably, the embodiment of the present invention applies also for each tool or miscellaneous calling and realization, and such as: it is online to carry out The method of Sequence Alignment, the online side for carrying out Nucleic Acid Dot Plot and return to multiple results simultaneously Method, the online implementation method for carrying out shRNA Target Design, the online realization side for carrying out Codon Optimization Method, carries out DNA Secondary Structure's at the online implementation method for carrying out GC Content Calculator online Implementation method and method (the Upload&View My for online converting standard biologic sequential file to Vector map displaying Vector)。

Correspondingly, the present embodiment applies also for the method that carrier framework is inserted into after pre-processing to shRNA sequence fragment；It is right The method of carrier framework is inserted into after the pretreatment of gRNA sequence；To the method for being inserted into carrier framework after the pretreatment of multiple ORF sequences； The judgment method of the biological effectiveness after carrier framework is inserted into complex sequence segment；According to user's sequence fragment dynamic generation The method of Vector map；Method (the Restriction enzyme site of restriction enzyme site analysis is carried out to carrier data online analysis)；The editor of the Protein tag of sequence fragment, online sequence fragment are searched and are marked online ORF and to sequence The name of segment.

As shown in figure 9, the present invention also provides a kind of method of carrier online trading, the method specifically includes as follows Step:

Step S300 generates the price list of corresponding demand carrier data；

Specifically, the valuation measurement includes the intelligently valuation measurement of manual invoicing measurement and system.

In embodiments of the present invention, valuation is measured as automatic price measurement and manual invoicing measures two parts.Automatic price Measurement, is to calculate the carrier and sequence of different biological features by special algorithm, obtain underlying price, in conjunction with default price, into Row plans as a whole quotation.Automatic price measurement is completed in Customer design, when obtaining design result, while generating.Artificial meter Valence measurement, is the more special personalized carrier outside automatic price measurement range, by submitting inquiry application, back-end technology Member carries out manual examination and verification and quotation.

Specifically, as shown in Figure 10, during front end sends vector construction processing completion instruction, backstage is available All data of building are provided with a quote program in this process from the background, and after this program, carrier may The quotation that can be obtained program setting and calculate is designed in carrier and is completed, and when returning to vector reporter interface, can be seen To the displaying of price.

Quote program can survey the type of carrier, the various evaluations of data progress of skeleton, raw material, technology path etc. Amount, so that calculating one offers or obtain the excessively complicated result that cannot be offered of carrier.

If carrier cannot offer automatically, an inquiry function can be listed in the order information column of the vector reporter page It can key.Front end can carry out inquiry to carrier by inquiry function and obtain artificial quotation.

Inquire the price function pages in, need to input title and the mail address of inquiry personnel with facilitate reply inquire the price as a result, this It is outer to be also an option that and input whether provide the carrier element template of insertion, attachment is uploaded, addition is personal to be described, to facilitate work Personnel offer.

In background interface, staff can obtain the inquiry data of front end submission, generate quotation simultaneously by manual evaluation Input saves,

At this time will it is optional whether instant transmission circular mail give inquiry personnel.If selection is sent, the postal for input of inquiring the price Part address will will receive a quotation notice, and content is that the carrier of notice inquiry has been offered.Regardless of whether notice is sent, It is current to open the front end vector reporter page, it can see that carrier has been offered.

In addition, background work personnel can be sent by e-mail contact and be ask if carrier is excessively complicated or data are wrong The personnel of valence link up.If confirming carrier data mistake, it will not offer, can choose redesign (i.e. on line Building processing) or notify that front end is redesigned.

In embodiments of the present invention, the price list of the corresponding demand carrier data of generation, as combines Shipping Address (for estimating freight charges), confirmation purchase article are combined with optionally preferential code using selection, after article is bought in final confirmation, then A quotation unirecord can be generated.

In embodiments of the present invention, it need to be bought in view of the sale of biotechnology class product, with traditional sale of goods mould Formula has very big difference, especially online trading pattern.VB has been worked out a set of feasible effective according to market and trading rules The trade management process of biotechnology class service product.

Specifically process of exchange is the article (design) that will can be bought first, is added to shopping cart as shown in figure 11. Shopping cart is divided into login and is not logged in two states, saves the price of article by server database and session respectively And essential information.When never logging state transforms to logging state, the data that position saves at two can merge and be saved in In server database.Cart page can choose and generate price list or directly purchase.

Price list is followed by generated, has generation quotation single function in cart page.Shipping Address is inputted (for estimating Freight charges), confirmation purchase article.There is optional preferential code using selection, after use, can obtain competitively priced.Confirmation purchase object After product, selection is generated, then can generate a quotation unirecord.The PDF document of price list can be downloaded.The inside enumerates quotation Single details, article details, price details, there are also some terms of service, the contents of points for attention etc..

After generating price list, it can choose and lower single operation is carried out to price list.Here there are two selections, and one is logical PO file is crossed to place an order, another is directly to carry out the purchase that places an order by credit card funded payment.Here direct purchase, and Direct purchase before generating price list, is the same logic.

It is placed an order by PO (legal document of similar contract) file, then needs to input a PO number, upload PO file, it is defeated Enter billing address, submits data, place an order.Order record can be generated after placing an order.Order contents are inherited from price list, increase Part order clause.Order can enter order auditing flow after generating, this when, the state of order was submission state.If Order audit not over, send advice audit and it is lower one link up.Such as cancellation of order, then it can change order status.It orders Single pass-through can then send order by notice, into production procedure.

It is directly bought by credit card, then can enter credit card funded payment process.Here third-party platform has been accessed Authorize.net carries out credit card gathering.After completing gathering, it will generate the order and completion status of pending state Invoice and bill.The auditing flow of order is as top PO file places an order.

After production is completed, dispatch list can established from the background.Article is packaged after delivery, can be sent delivery and be reminded.If It is outstanding order (being placed an order by PO file), then can generates and urge bill, is sent to buying side and carries out urging account.Urge bill payment Later, just payment bill can be generated on backstage, completes process.

Department is constructed under line, according to the confirmation data that quotation payment is completed, generates and constructs and deliver shape under demand carrier line State information trace list, until carrying out confirmation of examining goods in the delivery to client's hand of demand carrier, being finally completed demand carrier line and submit Easily.

Specifically, a kind of method of carrier online trading of the present invention, specifically comprises the following steps:

Step S300 generates the price list of corresponding demand carrier data；

Preferably, the embodiment of the present invention applies also at genophore commercial articles ordering and order based on 020 business model Reason and the method (Restriction enzymesite analysis) for carrying out restriction enzyme site analysis to carrier data online.

As shown in figure 12, be sixth preferred embodiment of the invention, a kind of method of carrier online trading, in step S300 it In, further include following steps:

Specifically, described to show that corresponding demand carrier price data includes showing interface demand carrier price data and mail Mode reminds the price data of demand carrier in real time.

If the quotation of demand carrier has been paid, S302 is thened follow the steps: obtaining approved quotation forms data, generate detection and close The downloading data of lattice and the demand carrier data synthesized；Otherwise step S301: corresponding demand carrier price data is shown, and real When check demand carrier data price list.

That is, during front end sends vector construction processing completion instruction, the institute of the available building in backstage There are data, in this process, is provided with a quote program from the background, after this program, carrier may obtain journey The quotation that sequence sets and calculates is designed in carrier and is completed, when returning to vector reporter interface, it can be seen that price Detail is shown；

In background interface, staff can obtain the inquiry data of front end submission, generate quotation simultaneously by manual evaluation Input saves, and real-time core offers to demand carrier data.

The downloading data of the demand carrier data for generating detection qualification and having synthesized, specially offer corresponding demand Carrier data is downloaded and is used to user.In addition, the products & services of VB, are had by complete technical support Complete technical documentation is downloaded and is used in order to provide user.

The specifically process of exchange of sixth preferred embodiment of the invention, as shown in figure 11, step detail is above It illustrates, details are not described herein again.

Specifically, sixth preferred embodiment of the invention specifically comprises the following steps:

Step S300 i.e. of the present invention, generates the price list of corresponding demand carrier data；

It is described to show that corresponding demand carrier price data includes that showing interface demand carrier price data and lettergram mode are real When remind demand carrier price data.

Preferably, the embodiment of the present invention applies also at genophore commercial articles ordering and order based on 020 business model Reason and the method (Restriction enzyme site analysis) for carrying out restriction enzyme site analysis to carrier data online.

As shown in figure 13, the present invention provides the system constructed on a kind of carrier line, the system is specifically included:

First acquisition unit, for obtaining demand carrier data；

Correspondingly, this system applies also for the method that carrier framework is inserted into after pre-processing to shRNA sequence fragment；It is right The method of carrier framework is inserted into after the pretreatment of gRNA sequence；To the method for being inserted into carrier framework after the pretreatment of multiple ORF sequences； The judgment method of the biological effectiveness after carrier framework is inserted into complex sequence segment；According to user's sequence fragment dynamic generation The method of Vector map；Method (the Restriction enzyme site of restriction enzyme site analysis is carried out to carrier data online analysis)；The editor of the Protein tag of sequence fragment, online sequence fragment are searched and are marked online ORF and to sequence The name of segment.

As shown in figure 14, the system constructed on a kind of carrier line further include:

First obtains module, for receiving list transmission data；

Preferably, as shown in figure 14, the first building processing unit includes:

Second obtains module, for obtaining demand carrier system data；

Preferably, as shown in figure 14, the second building processing unit includes:

Preferably, as shown in figure 14, the filtering detection unit includes:

As shown in figure 15, the present invention provides a kind of system of carrier online trading, the system includes:

Correspondingly, this system apply also for based on the genophore commercial articles ordering of 020 business model and order processing and Line carries out the method (Restriction enzyme site analysis) of restriction enzyme site analysis to carrier data.

Preferably, as shown in figure 16, the quotation generation unit in a kind of system of carrier online trading further include:

It as shown in figure 17, is the structural schematic diagram of terminal provided in an embodiment of the present invention.In a preferred embodiment of the present invention, The terminal 3 includes memory 31, at least one processor 32, at least one communication bus 33 and display screen 34.

It will be understood by a person skilled in the art that the structure of the terminal shown in Figure 17 does not constitute the limit of the embodiment of the present invention Fixed, either bus topology, is also possible to star structure, the terminal 3 can also include more more or fewer than illustrating Other hardware perhaps software or different component layouts.

In some embodiments, the terminal 3 includes that one kind can be automatic to carry out according to the instruction for being previously set or storing Numerical value calculates and/or the terminal of information processing, and hardware includes but is not limited to microprocessor, specific integrated circuit, programmable gate Array, digital processing unit, embedded device etc..The terminal 3 may also include customer equipment, and the customer equipment includes but unlimited The electricity of human-computer interaction can be carried out by modes such as keyboard, mouse, remote controler, touch tablet or voice-operated devices with client in any one Sub- product, for example, personal computer, tablet computer, smart phone, digital camera etc..

It should be noted that the terminal 3 is only for example, other electronic products that are existing or being likely to occur from now on such as may be used It is adapted to the present invention, should also be included within protection scope of the present invention, and is incorporated herein by reference.

In some embodiments, the memory 31 is used to store program code and various data, such as is mounted on described On carrier line in terminal 3 building and transaction system, and in the operational process of terminal 3 realize high speed, be automatically completed program Or the access of data.The memory 31 includes read-only memory (Read-OnlyMemory, ROM), random access memory (RandomAccessMemory, RAM), programmable read only memory (ProgrammableRead-OnlyMemory, PROM), Erasable Programmable Read Only Memory EPROM (ErasableProgrammableRead-OnlyMemory, EPROM), disposable programmable Read-only memory (One-timeProgrammableRead-OnlyMemory, OTPROM), electronics erasing type can make carbon copies read-only deposit Reservoir (Electrically-ErasableProgrammableRead-OnlyMemory, EEPROM), CD-ROM (CompactDiscRead-OnlyMemory, CD-ROM) or other disc memories, magnetic disk storage, magnetic tape storage or Person can be used in any other computer-readable medium of carrying or storing data.

In some embodiments, at least one described processor 32 can be made of integrated circuit, such as can be by single The integrated circuit of encapsulation is formed, and is also possible to be made of the integrated circuit that multiple identical functions or different function encapsulate, be wrapped Include one or more central processing unit (CentralProcessingunit, CPU), microprocessor, digital processing chip, figure The combination etc. of shape processor and various control chips.At least one described processor 32 is the control core of the terminal 3 (ControlUnit), it using all parts of various interfaces and the entire terminal 3 of connection, is stored in by running or executing Program or module in the memory 31, and the data being stored in the memory 31 are called, to execute terminal 3 Various functions and processing data, such as execute the function of constructing and trade on carrier line.

In some embodiments, at least one communication bus 33 be arranged to realize the memory 31, it is described extremely Connection communication between a few processor 32 and the display screen 34 etc..

In some embodiments, the display screen 34 can be used for showing the information by viewer's input or be supplied to viewing The information of person and the various figure viewer interfaces of terminal 3, these figures viewer interface can by figure, text, icon, Video and any combination thereof are constituted.The display screen 34 may include display panel, optionally, can use liquid crystal display Curtain (LiquidCrystalDisplay, LCD), Organic Light Emitting Diode (OrganicLight-EmittingDiode, OLED) Etc. forms configure display panel.

The display screen 34 can also include touch panel.If the display screen 34 includes touch panel, described Display screen 34 may be implemented as touch screen, to receive the input signal from viewer.Touch panel includes one or more A touch sensor is to sense the gesture on touch, slide, and touch panel.Above-mentioned touch sensor can not only sense touch Or the boundary of sliding action, but also detect duration and pressure relevant to above-mentioned touch or slide.The display Panel and the touch panel can be used as two independent components to realize input and input function, but in some embodiments In, the display panel and the touch panel can be integrated and realize and output and input function.

Although being not shown, the terminal 3 can also include the power supply (such as battery) powered to all parts, it is preferred that Power supply can be logically contiguous by electric power controller and at least one described processor 32, to pass through electric power controller reality The functions such as now management charging, electric discharge and power managed.Power supply can also include one or more direct current or alternating current The random components such as source, recharging device, power failure detection circuit, power adapter or inverter, power supply status indicator. The terminal 3 can also include multiple sensors, bluetooth module, Wi-Fi module etc., and details are not described herein.

It should be appreciated that the embodiment is only purposes of discussion, do not limited by this structure in patent claim.

The above-mentioned integrated unit realized in the form of software function module, can store and computer-readable deposit at one In storage media.Above-mentioned software function module is stored in a storage medium, including some instructions are used so that a computer Equipment (can be personal computer, terminal or the network equipment etc.) or processor (processor) execute each reality of the present invention Apply the part of the method.

In a further embodiment, in conjunction with Figure 17, the operation of the terminal 3 is can be performed at least one described processor 32 Device and the types of applications program of installation (building and transaction system on carrier line as mentioned), program code etc., for example, on The modules stated.

Program code is stored in the memory 31, and at least one described processor 32 can call the memory 31 The program code of middle storage is to execute relevant function.For example, modules described in system are stored in the memory Program code in 31, and as performed by least one described processor 32, to realize the function of the modules to reach The purpose for constructing and trading on to carrier line.

In one embodiment of the invention, the memory 31 stores multiple instruction, the multiple instruction by it is described extremely A few processor 32 is performed to realize the method for constructing and trading on carrier line.

In one embodiment of the invention, the processor 32 includes: to the execution of the multiple instruction

Construction method step on carrier line:

Step S10 obtains demand carrier data；

Carrier online trading method and step:

Step S300 generates the price list of corresponding demand carrier data；

The present invention also proposes a kind of computer-readable storage medium, as shown in figure 18, the computer-readable storage Media storage has the application program constructed on carrier line with method of commerce, and the application program is realized to be constructed on the carrier line And the step of method of commerce.Steps are as follows for corresponding specific method:

Construction method step on carrier line:

Step S10 obtains demand carrier data；

Carrier online trading method and step:

Step S300 generates the price list of corresponding demand carrier data；

Specifically, it is corresponding to can refer to Fig. 1 or Fig. 9 to the concrete methods of realizing of above-metioned instruction at least one described processor 32 The description of correlation step in embodiment, this will not be repeated here.

In several embodiments provided by the present invention, it should be understood that disclosed device, device and method can be with It realizes by another way.For example, the apparatus embodiments described above are merely exemplary, for example, the module It divides, only a kind of logical function partition, there may be another division manner in actual implementation.

The module as illustrated by the separation member may or may not be physically separated, aobvious as module The component shown may or may not be physical unit, it can and it is in one place, or may be distributed over multiple In network unit.Some or all of the modules therein can be selected to realize the mesh of this embodiment scheme according to the actual needs 's.

It, can also be in addition, each functional module in each embodiment of the present invention can integrate in one processing unit It is that each unit physically exists alone, can also be integrated in one unit with two or more units.Above-mentioned integrated list Member both can take the form of hardware realization, can also realize in the form of hardware adds software function module.

It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims Variation is included in the present invention.Any reference signs in the claims should not be construed as limiting the involved claims.This Outside, it is clear that one word of " comprising " is not excluded for other units or, odd number is not excluded for plural number.The multiple units stated in device claim Or device can also be implemented through software or hardware by a unit or device.

The present invention passes through building on a kind of carrier line and each step of method traded and each functional unit of system and function Module can allow researcher easily to carry out online ordering personalization DNA vector and related reagent according to Research Requirements.It should Method and system can provide simplified carrier design cycle, and user, which only needs to click several lower mouses, can freely obtain carrier Design scheme, while other relevant services with the carrier, such as carrier cloning and virus packaging can also be customized.

In addition, the product of biological gene carrier is service class product, the demand of each client has its biology unique, So the shopping process of this kind of product and conventional entity product shopping process have very big difference.Client needs voluntarily or in work Cheng Shi designs requirement objective carrier under helping.The processes such as quality inspection could be produced and be verified on the basis of this design, Finally article could be delivered to client on hand.And in this process, carrier designs this link, the even more most important thing. When designing completion, client should just obtain information specific enough to confirm expected result, this design contains The biological nature of customer demand product is one and general contains the result of biological information.In this regard, VectorBuilder is opened Invasive has accomplished all information on webpage, has developed a set of simple and convenient carrier by Integration of Information Technology and designs work Tool and purchase platform.

And carrier is generally only the first step of experiment demand, many times needs to carry out more downstream services, or More matched Extension Services are needed, available product can be just obtained.VB is according to industry requirement, by technical research, for not Same carrier basis, sets a variety of default downstream services.

The downstream service includes: that plasmid extracts, and virus packaging, mRNA is extracted, and gRNA is extracted, host transformation；

The Extension Services include: control vector, control virus, mating assistant carrier.

The method of the present invention and system realize letter for APP in carrier design lines and specific process combination human-computer interaction Change carrier design logic mode on convenient and fast line；Entire industrial design process, all uniquenesses of price and transaction are designed on line Design and bioscience technology document.

The present invention works out and has started the online building and transaction system of a set of carrier and adequate and systematic service, can make gene The policy specification and standardization of carrier design, and building process is simple, designer and producer's rational division of labor, thus significantly It shortens construction schedule and improves production efficiency, while also achieving the efficient customization of biotechnology service product, save use A large amount of manpower and material resources of family voluntarily DIY.This logical process has also standardized the production and transaction flow of itself, is allowed to more rationally With it is efficient.

The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.

Claims

1. a kind of method constructed on carrier line, which is characterized in that the method specifically comprises the following steps:

Step S10 obtains demand carrier data；

2. the method constructed on a kind of carrier line according to claim 1, which is characterized in that the demand carrier data packet Include demand carrier type data information and demand carrier framework data information；

It is described to carry out corresponding demand carrier data selection and first time building processing, specifically: to the selection of list, gene is believed The search and insertion of breath, search and insertion to sequence data；

To chosen and building for the first time treated demand carrier carries out editor and second of building processing, specifically: addition Sequence label and sequence is edited or is modified.

3. the method constructed on a kind of carrier line according to claim 1, which is characterized in that before step S10, also wrap Include following steps:

Step S00 receives list and transmits data；

List transmission data are saved into database, and carry out scheme demand determination processing for form data by step S01；

Step S02, by determination processing result data real-time informing vector construction demander；

The list transmission data include the text data and optional upload file data of selected service type data, input；

4. the method constructed on a kind of carrier line according to claim 1, which is characterized in that among step S20, also wrap Include following steps:

Step S21 obtains demand carrier system data；

Step S24 determines chosen and building for the first time treated whether demand carrier carry out editor and second of structure Build processing；It is no to then follow the steps S40 if so then execute step S30.

5. the method constructed on a kind of carrier line according to claim 1, which is characterized in that among step S30, also wrap Include following steps:

6. the method constructed on a kind of carrier line according to claim 1, which is characterized in that among step S40, also wrap Include following steps:

Whether step S41, the demand carrier sense for determining that building processing is completed are qualified；If qualification thens follow the steps S50, otherwise hold Row step S20.

7. a kind of method of carrier online trading, which is characterized in that the method specifically comprises the following steps:

Step S300 generates the price list of corresponding demand carrier data；

Step S400, according to quotation payment complete confirmation data, generate demand carrier line under construct and delivery status information with Track list is finally completed demand carrier online trading；

8. a kind of method of carrier online trading according to claim 7, which is characterized in that among step S300, also Include the following steps:

Step S302 obtains approved quotation forms data, generates the downloading for detecting demand carrier data that is qualified and having synthesized Data；

It is described to show that corresponding demand carrier price data includes that showing interface demand carrier price data and lettergram mode mention in real time The price data of awake demand carrier.

The real-time core to demand carrier data price list include: in real time to demand carrier carry out quotation detail check and approve and in real time it is right Whether the quotation of demand carrier, which is completed payment, is checked and approved；

9. the system constructed on a kind of carrier line, which is characterized in that the system includes:

First acquisition unit, for obtaining demand carrier data；

First building processing unit carries out corresponding demand carrier data selection and first time structure for carrier data according to demand Build processing；

Second building processing unit, for chosen and building for the first time treated demand carrier carries out editor and second Building processing；

10. the system constructed on a kind of carrier line according to claim 9, which is characterized in that the system further include:

First obtains module, for receiving list transmission data；

First determination module for saving list transmission data into database, and carries out scheme demand for form data Determination processing；

Notification module is used for determination processing result data, real-time informing vector construction demander；

Described first constructs processing unit

Second obtains module, for obtaining demand carrier system data；

Insertion process module is selected or is inserted into demand carrier for combining the gene data and sequence data that search Processing；

Second determination module, treated for determining chosen and building for the first time whether demand carrier carry out editor and Second of building processing；

Described second constructs processing unit

First display module, the demand carrier data completed for showing editor or modification；

The filtering detection unit includes:

11. a kind of system of carrier online trading, which is characterized in that the system includes:

Measuring unit of valuating carries out valuation measurement for the type detail according to the demand carrier that detection is qualified and has synthesized；

Status tracking unit constructs and delivers shape for generating according to the confirmation data that quotation payment is completed under demand carrier line State information trace list is finally completed demand carrier online trading.

12. a kind of system of carrier online trading according to claim 11, which is characterized in that the system also includes:

It shows and checks and approves module, for showing corresponding demand carrier price data, and real-time core is to demand carrier data price list；

Generation module is downloaded, for obtaining approved quotation forms data, generates the demand number of carriers that detection is qualified and has synthesized According to downloading data；

Confirm sending module, completes confirmation data for the payment that will offer and be sent to building portion under corresponding demand carrier line Door.

13. the platform for constructing and trading on a kind of carrier line characterized by comprising

Wherein the platform courses program, the platform courses program for constructing and trading on the carrier line are executed in the processor It is stored in the memory, the platform courses program for constructing and trading on the carrier line realizes such as claim 1 to 8 Any one of described in carrier line on construct and transaction method and step.

14. a kind of computer-readable storage medium, which is characterized in that the computer-readable storage medium is stored with carrier The platform courses program for constructing and trading on line, constructs on the carrier line and the platform courses program of transaction, realizes such as right It is required that the method and step for constructing and trading on carrier line described in any one of 1 to 8.