CN115975770A - Enrichment device of microorganism in environmental sample - Google Patents
Enrichment device of microorganism in environmental sample Download PDFInfo
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- CN115975770A CN115975770A CN202211730705.XA CN202211730705A CN115975770A CN 115975770 A CN115975770 A CN 115975770A CN 202211730705 A CN202211730705 A CN 202211730705A CN 115975770 A CN115975770 A CN 115975770A
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- 244000005700 microbiome Species 0.000 title claims abstract description 44
- 230000007613 environmental effect Effects 0.000 title claims abstract description 33
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- 239000012528 membrane Substances 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 3
- 239000012780 transparent material Substances 0.000 claims description 3
- 239000001963 growth medium Substances 0.000 abstract description 25
- 238000009630 liquid culture Methods 0.000 abstract description 18
- 241000894006 Bacteria Species 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 4
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- 238000000034 method Methods 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 6
- 238000012986 modification Methods 0.000 description 5
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- 235000015097 nutrients Nutrition 0.000 description 5
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- 241001052560 Thallis Species 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
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- 239000012466 permeate Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
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- 239000002689 soil Substances 0.000 description 2
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
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Abstract
The invention belongs to the technical field of microorganism culture devices, and particularly relates to an enrichment device for microorganisms in an environmental sample. The enrichment device includes the box body, box body top uncovered department lid has closed the lid, be equipped with the enrichment subassembly in the box body, the enrichment subassembly includes double-deck rack, bottom cavernosum and top layer cavernosum, the bottom cavernosum with the top layer cavernosum is all placed the upper strata of double-deck rack, and the top layer cavernosum place in on the top surface of bottom layer cavernosum. The enrichment device provided by the invention adopts the liquid culture medium to provide nutrition, not only provides better conditions for enrichment culture of the mold, but also primarily separates the mold from other bacteria which do not produce hypha.
Description
Technical Field
The invention belongs to the technical field of microorganism culture devices, and particularly relates to an enrichment device for microorganisms in an environmental sample.
Background
The detection of environmental microorganisms is an important experimental operation for evaluating environmental ecology, environmental quality and screening microorganisms with specific functions, common environmental samples include air environments, soil environments, water environments and the like, wherein the soil samples or the water samples are common sample sources for carrying out microorganism screening, especially samples under special conditions such as organic matter polluted sludge and the like, and microorganisms with specific functions such as degraded organic matters and the like are often contained in the samples, so that the microorganisms are enriched and separated, and the development of a microbial preparation for improving the environment is facilitated.
Currently, the common microorganism enrichment method is a culture method, i.e., a selective culture medium is used to screen out a class of target microorganisms, and then plate coating separation is performed (Zhangjing, liu Xiao Ying, qian Cheng, research progress of the microorganism enrichment method and application thereof [ J ] microorganism and infection, 2010,005 (003): 176-180 [2] Johnny image, enrichment method of plant-related microorganisms and molecular characteristics of diversity thereof [ D ] Kunming plant institute of Chinese academy of sciences, 2005 ]. The device used in the enrichment method comprises a triangular flask and a plate, wherein a liquid culture medium is filled in the triangular flask and mainly plays a role in enrichment, and a solid culture medium is filled in the plate and mainly plays a role in separation.
Microorganisms in the environment comprise bacteria, moulds and the like, many moulds comprise aerial hyphae and nutritional hyphae, and due to the existence of the hyphae, the liquid culture medium enrichment method in the prior art is not friendly to the growth of the aerial hyphae of the moulds and is not beneficial to spore production and reproduction. Therefore, the invention develops a device which adopts a liquid culture medium to provide nutrition but is beneficial to the growth of aerial hyphae.
Disclosure of Invention
In order to solve the technical problem, the invention provides a device for enriching microorganisms in an environmental sample.
The invention aims to provide an enrichment device for microorganisms in an environmental sample, which comprises a box body, wherein a box cover is covered at an opening at the top of the box body, an enrichment assembly is arranged in the box body, the enrichment assembly comprises a double-layer net rack, a bottom layer sponge body and a top layer sponge body, the bottom layer sponge body and the top layer sponge body are both arranged on the upper layer of the double-layer net rack, and the top layer sponge body is arranged on the top surface of the bottom layer sponge body.
Preferably, the box cover is formed by splicing double-layer nets, and cotton is inlaid between the double-layer nets.
Preferably, in the device for enriching microorganisms in the environmental sample, an adsorption film is placed on the lower layer of the double-layer net rack.
Preferably, in the apparatus for enriching microorganisms in an environmental sample, the adsorption membrane is a water-permeable filter membrane.
Preferably, the enrichment device of microorganism in the above-mentioned environment sample, double-deck rack includes the shell, the shell only has open-top and bottom opening, the inside bottom opening department that is located the shell of shell is equipped with the bottom net, bottom net upper surface has been laid the adsorption film, the shell inner wall, be located the department can be dismantled and be equipped with the top net in adsorption film top, the top has shelved on the net the bottom cavernosum, shelved on the bottom cavernosum the top sponge.
Preferably, in the device for enriching microorganisms in the environmental sample, a boss is arranged on the inner wall of the housing and above the adsorption film, and the top layer net is placed on the boss.
Preferably, in the device for enriching microorganisms in environmental samples, the distance between the upper surface of the top sponge body and the upper edge of the top opening of the shell is 3-8cm.
Preferably, in the apparatus for enriching microorganisms in an environmental sample, the upper surface of the bottom sponge body is provided with a pit, and the bottom surface of the top sponge body is provided with a protrusion at a position corresponding to the pit.
Preferably, the enrichment facility of microorganism in above-mentioned environment sample, the inner wall symmetry of box body is equipped with the hangers, double-deck rack surface both sides all are fixed with the suspension rope, deviating from of suspension rope the one end of double-deck net rather than the homonymy the hangers can be dismantled and be connected.
Preferably, the box body and the box cover are made of transparent materials.
Compared with the prior art, the invention has the following beneficial effects:
1. the enrichment device provided by the invention adopts the liquid culture medium to provide nutrition, so that better conditions are provided for enrichment culture of the mold, and the mold is preliminarily separated from other bacteria which do not produce hyphae. Through setting up the adsorption film, there is the adsorption of preferred to the thallus, after the cultivation, take off the adsorption film, can collect the thallus on it, need not centrifugal culture solution, the energy saving, the adsorption film can adopt pervious filter membrane etc.. The design of the double-layer net rack with the double-layer structure enables the lower part of the top-layer cavernous body to be fully contacted with a liquid culture medium, so that nutrients can be conveniently moved, precipitated thalli can be separated from the top-layer cavernous body, and the collection of subsequent thalli is facilitated.
2. In the prior art, a solid culture medium is usually adopted for culturing the mould, and a large amount of heat generated by microbial propagation is particularly difficult to dissipate in the solid culture medium, so that the operation of shaking the flask and turning the mould is needed.
3. According to the invention, the concave pits and the convex bumps are arranged, so that liquid substances of the environmental sample are inoculated into the concave pits, and the sample is prevented from splashing. The double-layer net rack can be suspended in the box body by arranging the hangers, the suspension ropes and the hanging buckles, and the bottom of the double-layer net rack can be fully contacted with nutrients of a liquid culture medium.
Drawings
FIG. 1 is a schematic perspective view of an enrichment apparatus according to example 1 of the present invention;
FIG. 2 is a schematic longitudinal sectional view of an enrichment apparatus in example 1 of the present invention;
FIG. 3 is a schematic longitudinal sectional view of an enrichment apparatus in example 2 of the present invention;
fig. 4 is a schematic longitudinal sectional view of an enrichment apparatus in example 3 of the present invention.
Detailed Description
In order that those skilled in the art will better understand the technical solutions of the present invention to be implemented, the present invention will be further described with reference to the following specific embodiments and accompanying drawings.
In the description of the present invention, reagents used are commercially available and methods used are conventional in the art, unless otherwise specified.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "transverse," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, but are not intended to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and are not to be construed as limiting the invention. The terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature; in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Example 1
The utility model provides an enrichment facility of microorganism among environmental sample, refers to figure 1-2, including box body 1, be used for holding liquid medium in the box body 1, this liquid medium can enrich the purpose microorganism, for example place the selectivity liquid medium that can enrich aspergillus oryzae mould, and 1 top of box body is uncovered, and all the other surfaces are the confined surface, and 1 top of box body uncovered department lid has closed lid 2, and for the ease of observing the interior situation of box body 1, box body 1 and lid 2 adopt transparent material to prepare. The box cover 2 can be a cover formed by splicing double-layer nets, and cotton is inlaid between the double-layer nets, so that sterilization is facilitated.
Be equipped with the enrichment subassembly in box body 1, the enrichment subassembly includes double-deck rack 3, bottom cavernosum 4 and top layer cavernosum 5, and bottom cavernosum 4 and top layer cavernosum 5 are all placed in the upper strata of double-deck rack 3 to top layer cavernosum 5 is placed on the top surface of bottom cavernosum 4, and adsorption film 6 has been placed in the lower floor tiling of double-deck rack 3.
Since one of the preliminary steps of the microbial culture test is sterilization of the culture medium and the culture apparatus, the present embodiment is provided with two sterilization methods: firstly, placing a liquid culture medium in a box body 1, then covering a box cover 2, sterilizing by adopting high-pressure steam, wrapping an enrichment component by newspaper, then sterilizing by adopting high-pressure steam, and sterilizing and drying the enrichment component for use; secondly, liquid culture medium is placed in the box body 1, then the enrichment assembly is placed in the box body 1, the liquid level of the liquid culture medium is lower than the upper surface of the bottom layer cavernous body 4, then the box cover 2 is covered to sterilize by high-pressure steam, and the liquid culture medium can wet the bottom layer cavernous body 4 and the top layer cavernous body 5 in the sterilization process, but the inoculation use is not influenced.
The working principle of the enrichment device provided by the invention is as follows:
if the first sterilization mode is adopted, a liquid culture medium is placed in the box body 1, the enrichment assembly is placed in the box body 1, the liquid level of the liquid culture medium is lower than the upper surface of the bottom sponge body 4, and the top sponge body 5 is clamped by tweezers; if the second sterilization mode is adopted, the top sponge body 5 is clamped by direct tweezers; after the tweezers clamp the top sponge body 5, the environmental sample to be enriched is added on the upper surface of the bottom sponge body 4, then the top sponge body 5 is covered, the box cover 2 is covered, the inoculation work is completed, and the whole device is put into an incubator for cultivation. Since the bottom sponge 4 and the top sponge 5 are both made of porous sponge, the device has the functions of permeation and water absorption, on one hand, a liquid culture medium enters the bottom sponge 4 and the top sponge 5, on the other hand, microorganisms in an environment sample permeate the bottom sponge 4 and the top sponge 5 and even permeate the liquid culture medium, the microorganisms absorb nutrients of the liquid culture medium and breed in a proper environment of the incubator, as the top sponge 5 is above the liquid level, the growth of aerial hyphae and vegetative hyphae of mold is not limited, and bacteria, fungi and the like move to the bottom sponge 4 and even the lower layer of the double-layer net rack 3 under the action of gravity and are adsorbed on the adsorption film 6 or sink into the box body 1, so the enrichment device provided by the invention not only provides a better condition for enrichment culture of the mold, but also primarily separates the mold from other bacteria which do not produce hyphae. Through setting up adsorption film 6, there is the adsorption of preferred to the thallus, after the cultivation, take off adsorption film 6, can collect the thallus on it, need not centrifugal culture solution, the energy saving, adsorption film 6 can adopt pervious filter membrane etc.. The design of the double-layer net rack 3 with the double-layer structure enables the lower part of the top-layer sponge body 5 to be fully contacted with a liquid culture medium, so that nutrients can be conveniently moved, precipitated thalli can be separated from the top-layer sponge body 5, and the collection of subsequent thalli is facilitated.
In the prior art, a solid culture medium is usually adopted for culturing the mould, and a large amount of heat generated by microbial propagation is particularly in the solid culture medium and is difficult to dissipate, so that the operation of shaking the flask and turning the mould is needed.
Preferably, the double-layer net rack 3 comprises a cylindrical shell 31, the shell 31 only has a top opening and a bottom opening, a bottom net 32 is arranged inside the shell 31 and located at the bottom opening, an adsorption film 6 is laid on the upper surface of the bottom net 32, a boss is arranged on the inner wall of the shell 31 and located above the adsorption film 6, the boss is an annular table, a top net 33 is placed on the boss, a bottom sponge 4 is placed on the top net 33, a top sponge 5 is placed on the bottom sponge 4, and the top net 33, the bottom sponge 4 and the top sponge 5 are arranged in the shell 31. Because shell 31 only has seted up open-top and bottom opening, so after bottom layer cavernosum 4 upper surface inoculation environment sample, notice top layer cavernosum 5 upper surface and shell 31 open-top upper edge (that is the shell 31 top surface) between the distance, set up to 3-8cm, then the microorganism grows in the within range of shell 31, almost not only get into the liquid medium of box body 1, after the cultivation, directly remove whole enrichment subassembly from box body 1, collect the microorganism on it can, reduce centrifugal operation.
Example 2
An apparatus for enriching microorganisms in an environmental sample, having substantially the same structure as that of example 1, except that, referring to fig. 3, if the environmental sample is a liquid substance dissolved in water and scattered by glass beads, a concave 41 is dug in the middle of the upper surface of the bottom sponge 4, a protrusion 51 is provided at a position corresponding to the concave 41 on the bottom surface of the top sponge 5, and the liquid substance of the environmental sample is inoculated into the concave 41 to prevent the sample from splashing. The small box of figure 3 is the inoculation location for the environmental sample.
Example 3
An enrichment device of microorganisms in environmental samples has the same structure as that of the embodiment 2, and the difference is that referring to fig. 4, the inner wall of a box body 1 is symmetrically provided with hanging lugs 11, two sides of a double-layer net rack 3 are both fixed with one end of a hanging rope 34, the other end of the hanging rope 34 is fixed with a hanging buckle 35, and the hanging buckle 35 can be hung on the hanging lug 11 on the same side in a matching way.
The purpose of arranging the hangers 11, the hanging ropes 34 and the hanging buckles 35 is to suspend the double-layer net rack 3 in the box body 1, so that the bottom of the double-layer net rack 3 can be fully contacted with the nutrients of the liquid culture medium.
It should be noted that, the connection relationship of the components not specifically mentioned in the present invention is the default of the prior art, and the connection relationship of the structure is not described in detail since it does not relate to the invention point and is a common application of the prior art.
It should be noted that, when the present invention relates to a numerical range, it should be understood that two endpoints of each numerical range and any value between the two endpoints can be selected, and since the steps and methods adopted are the same as those in the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including the preferred embodiment and all changes and modifications that fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. The utility model provides an enrichment device of microorganism in environmental sample, a serial communication port, includes box body (1), box body (1) top opening department lid has closed lid (2), be equipped with the enrichment subassembly in box body (1), the enrichment subassembly includes double-deck rack (3), bottom cavernosum (4) and top layer cavernosum (5), bottom cavernosum (4) with top layer cavernosum (5) are all placed the upper strata of double-deck rack (3), and top layer cavernosum (5) place in on the top surface of bottom cavernosum (4).
2. The device for enriching microorganisms in environmental samples according to claim 1, wherein the box cover (2) is formed by splicing double-layer nets, and cotton is inlaid between the double-layer nets.
3. The device for enriching microorganisms in environmental samples according to claim 1, wherein an adsorption film (6) is placed on the lower layer of the double-layer net rack (3).
4. The device for enriching microorganisms in environmental samples according to claim 3, wherein the adsorption membrane (6) is a water-permeable filter membrane.
5. The device for enriching microorganisms in environmental samples according to claim 3, wherein the double-layer net rack (3) comprises a shell (31), the shell (31) is only provided with a top opening and a bottom opening, a bottom net (32) is arranged inside the shell (31) and positioned at the bottom opening, the adsorption film (6) is laid on the upper surface of the bottom net (32), a top net (33) is detachably arranged on the upper part of the adsorption film (6) on the inner wall of the shell (31), the bottom sponge (4) is placed on the top net (33), and the top sponge (5) is placed on the bottom sponge (4).
6. The device for enriching microorganisms in environmental samples according to claim 5, wherein a boss is arranged on the inner wall of the shell (31) above the adsorption film (6), and the top layer mesh (33) is placed on the boss.
7. The apparatus for the enrichment of microorganisms in environmental samples according to claim 5, wherein the distance between the upper surface of the top sponge (5) and the upper edge of the top opening of the housing (31) is 3-8cm.
8. The device for enriching microorganisms in an environmental sample according to claim 1, wherein the bottom sponge body (4) is provided with a concave pit (41) on the upper surface, and the top sponge body (5) is provided with a convex pit (51) on the bottom surface corresponding to the concave pit (41).
9. The device for enriching the microorganisms in the environmental samples according to claim 1 or 8, wherein the inner wall of the box body (1) is symmetrically provided with hanging lugs (11), two sides of the outer surface of the double-layer net rack (3) are respectively fixed with a hanging rope (34), and one end of the hanging rope (34) departing from the double-layer net rack (3) is detachably connected with the hanging lugs (11) on the same side.
10. The apparatus for enriching microorganisms in environmental samples according to claim 1, wherein the box body (1) and the box cover (2) are made of transparent materials.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211730705.XA CN115975770A (en) | 2022-12-30 | 2022-12-30 | Enrichment device of microorganism in environmental sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211730705.XA CN115975770A (en) | 2022-12-30 | 2022-12-30 | Enrichment device of microorganism in environmental sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115975770A true CN115975770A (en) | 2023-04-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211730705.XA Withdrawn CN115975770A (en) | 2022-12-30 | 2022-12-30 | Enrichment device of microorganism in environmental sample |
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| Country | Link |
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| CN (1) | CN115975770A (en) |
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2022
- 2022-12-30 CN CN202211730705.XA patent/CN115975770A/en not_active Withdrawn
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