CN115969730A - Composition for tightening and lifting skin and preparation method and application thereof - Google Patents

Abstract

A composition for tightening and lifting skin and a preparation method and application thereof relate to the field of cosmetics, and the composition comprises the following components: peptide component (b): retinol component: surfactant (b): lecithin: the mass ratio of the cholesterol is (0.1-5): 0.05-5): 0.1-5): 2-10): 1-5. According to the composition for tightening and pulling the skin, after the peptide component and the retinol component are compounded according to a certain proportion, the retinol component can not only remarkably promote the permeability of the peptide component, but also play a role of the retinol component in the aspect of skin aging resistance; the composition is subjected to nano-coating, the retinol component is embedded in the middle of a phospholipid bilayer, and the peptide component is coated in the vesicle to form a multi-layer stable structure, so that the irritation and photodecomposition of retinol are avoided, the composition is favorably dispersed in a formula, and the stability of the composition is improved.

Description

Composition for tightening and lifting skin and preparation method and application thereof

Technical Field

The invention relates to the field of cosmetics, in particular to a composition for tightening and lifting skin, a preparation method and application thereof.

Background

Skin aging and relaxation are a big problem affecting the image, and people have stronger demand for compact lifting products. The collagen of the skin is continuously lost due to the age increase and the influence of gravity, so that the skin is increasingly loose; at present of economic development, under severe social life pressure, irregular work and rest and night-out become normal states, and skin aging is continuously stimulated; in addition, external pollution and ultraviolet irradiation are added, so that the process of skin aging is aggravated. Although the natural growth state and the environment of the skin cannot be changed, the skin care product with the function of tightening and lifting can be used for achieving the purpose of immediately shaping the facial contour, so that the skin looks younger and is wonderful.

A part of polypeptides such as acetyl hexapeptide-8, acetyl hexapeptide-1, etc. can act on presynaptic membrane to prevent acetylcholine release by blocking the process of fusion of synaptic vesicles with nerve endings. A small part of peptide components such as snake-like toxin peptide and conotoxin peptide not only act on presynaptic membrane, but also can be combined with acetylcholine receptor on postsynaptic membrane at neuromuscular junction. The peptide component acting on the acetylcholine receptor can block nerve excitation, act on a muscle relaxation channel, relieve tight muscles, and have the effects of safely and immediately fading wrinkles and tightening and lifting. However, due to the existence of skin barrier, the upper limit of the normal skin percutaneous absorption is 500 daltons, the skin absorption capacity begins to decline rapidly at about 600 daltons, and most of the peptide components acting on the acetylcholine exceed the skin absorption limit.

Most of the products on the market at present are mainly designed to have a long-acting anti-aging concept, and only few products are focused on instant tightening and lifting. At present, peptide components are mostly used for immediate compaction, but a better effect cannot be achieved due to permeability, and the higher the activity is, the stronger the efficacy is, the lower the bioavailability of the components is, the poorer the stability is, and the poorer the compatibility of the formula is. Therefore, a composition which is instantly compact, meets special requirements, and improves the problem of skin relaxation and has stability and high permeability needs to be solved urgently.

Disclosure of Invention

In order to overcome the disadvantages of the prior art, it is an object of the present invention to provide a skin-tightening and lifting composition that increases the permeability of peptide ingredients.

The invention also aims to provide a preparation method of the composition for tightening and lifting the skin, which has a simple preparation process.

The invention also aims to provide application of the composition for tightening and lifting skin.

One of the purposes of the invention is realized by adopting the following technical scheme:

a composition for tightening and lifting skin comprises the following components: peptide component (b): the mass ratio of the retinol components is (0.1-5) to (0.05-5).

Preferably, the composition for tightening and lifting skin has the mass ratio of the peptide component to the retinol component of (1-2) to 1.

Preferably, the peptide component is one or a combination of more than two of acetyl hexapeptide-3, acetyl hexapeptide-8, acetyl hexapeptide-1, acetyl tetrapeptide-5, acetyl tetrapeptide-9, snake venom like peptide, conotoxin peptide or scorpion toxin peptide.

Preferably, the retinol component is one or a combination of more than two of retinol, retinoic acid, hydrogenated retinol, retinol retinoic acid ester, retinol acetate, retinol palmitate, retinol linoleate, retinol propionate, retinol retinoic acid ester, or hydroxy pinacolone retinoic acid ester (HPR).

Preferably, the composition for tightening and lifting skin comprises the following components: peptide component (b): retinol component: surfactant (b): lecithin: the mass ratio of the cholesterol is (0.1-5): 0.05-5): 0.1-5): 2-10): 1-5.

Preferably, the surfactant is one or a composition of more than two of PEG-40 hydrogenated castor oil, polyvinylpyrrolidone, caprylic/capric triglyceride, tri (laureth-4) phosphate, polyglycerol-10 myristate or isosorbide dimethyl ether; the lecithin is one or more of soybean lecithin, egg yolk lecithin and hydrogenated substance thereof.

The second purpose of the invention is realized by adopting the following technical scheme:

a preparation method of a composition for tightening and lifting skin comprises the following steps:

s1: mixing and diffusing a retinol component, lecithin, cholesterol and an organic solvent, evaporating to dryness and drying to obtain a first mixture;

s2: adding a peptide component and a surfactant into the first mixture for hydration and diffusion to obtain a second mixture; and homogenizing the second mixture at high pressure by using micro-jet to prepare the composition for tightening and lifting the skin.

Preferably, the organic solvent is absolute ethyl alcohol or diethyl ether; the evaporation temperature in the step S1 is 15-30 ℃; and in the step S2, the speed of the high-pressure homogenization of the micro jet is 800-1000r/min, and the pressure is 800-1200bar.

The third purpose of the invention is realized by adopting the following technical scheme:

the application of the composition for tightening and lifting the skin is to prepare the skin care product.

Preferably, the skin care product is essence which comprises the following components in percentage by mass: 0.5-20% of composition for tightening and lifting skin, 0.1-0.5% of thickening agent, 5-20% of humectant, 0.5-10% of emollient, 0.1-3% of skin conditioner, 0.1-3% of emulsifier, 1-5% of preservative, 0.05-0.2% of essence and the balance of water.

Compared with the prior art, the invention has the beneficial effects that:

(1) According to the composition for firming and lifting skin, after the peptide component and the retinol component are compounded according to a certain proportion, the retinol component can not only remarkably promote the permeability of the peptide component, but also play a role of the retinol component in the aspect of skin aging resistance; the composition is subjected to nano-coating, the retinol component is embedded in the middle of a phospholipid bilayer, and the peptide component is coated in the vesicle to form a multi-layer stable structure, so that the irritation and photodecomposition of retinol are avoided, the composition is favorably dispersed in a formula, and the stability of the composition is improved.

(2) The preparation method of the composition for tightening and lifting skin can prepare the composition, and the peptide component and the retinol component are wrapped in the nano liposome vesicle; the retinol component is located at the outer layer of liposome and is released before the peptide component, so as to improve the transdermal absorption rate of the peptide component and increase the absorption time of skin for the released substance.

(3) The composition for tightening and lifting the skin is added into the skin care product, so that the microenvironment of a dermal extracellular matrix can be improved, and the permeability of cell membrane water and the absorption of nutrient substances by the skin can be increased.

Drawings

FIG. 1 is a graph showing the results of a irritation test of essences prepared in example 1 of the present invention and comparative example 5;

FIG. 2 shows the nerve soothing (tightening and wrinkle resisting) results of the essences prepared in examples 1 to 3 of the present invention and comparative examples 1 to 4 on zebrafish;

FIG. 3 is a graph showing the instant tightening effect of the essences prepared in example 2 and comparative example 3 of the present invention;

fig. 4 is a graph showing the effect of improving the long-lasting skin texture of the essences prepared in example 2 and comparative example 3 of the present invention.

Detailed Description

The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, various embodiments or technical features described below may be arbitrarily combined to form a new embodiment.

The molecular weight of the peptide components exceeds 600 daltons, and the principle of skin permeation of 500 daltons is broken through, so that the use income is low. The retinol component has small molecular weight and is easy to absorb, and can be converted into retinoic acid after entering a human body, so that the retinoic acid component has the function of exfoliating cutin, so that the channel of the skin is opened, and the retinoic acid component has strong irritation to the skin, which is the disadvantage of the retinol component. The retinol is positioned on the outer layer of the liposome and is released before the peptide component, and the retinol can increase the expression of the aquaporin 3 in the normal human skin, improve the microenvironment of dermal extracellular matrix, increase the water permeability of cell membranes and the absorption of nutrients by the skin, so the retinol component is used as the penetration enhancer of the peptide component, and the characteristic of the retinol is used for promoting the peptide component to enter the bottom layer of the skin. Experiments show that the wrapped retinol component can improve the transdermal absorption rate of polypeptide, increase the absorption time of skin to released substances, prolong the action time, improve the facial contour and the firmness more than that of the skin used alone, improve the skin elasticity and the firmness by 3 times than that of the skin used alone, and achieve the aims of synergism, decrement synergism and waste recycling.

In a first aspect of the present invention, there is provided a firming and skin-lifting composition comprising the following components: peptide component (b): the mass ratio of retinol components is (0.1-5) to (0.05-5).

In one embodiment, the composition for tightening and lifting skin has a mass ratio of the peptide component to the retinol component of (1-2): 1.

In one embodiment, the composition for tightening and lifting skin comprises the peptide component and the retinol component in a mass ratio of 1.

In one embodiment, the peptide component is a combination of one or more of acetyl hexapeptide-3, acetyl hexapeptide-8, acetyl hexapeptide-1, acetyl tetrapeptide-5, acetyl tetrapeptide-9, a snake venom-like peptide, a conotoxin peptide, or a erythrosin peptide.

In one embodiment, the retinol-based ingredient is one or a combination of more than two of retinol, retinoic acid, hydrogenated retinol, retinol retinoic acid ester, retinol acetate, retinol palmitate, retinol linoleate, retinol propionate, retinol retinoic acid ester, or hydroxy pinacolone retinoic acid ester (HPR).

In one embodiment, a skin tightening and lifting composition is encapsulated with nanoliposomes.

In one embodiment, the composition for tightening and lifting skin comprises the following components: peptide component (c): retinol component: surfactant (b): lecithin: the mass ratio of the cholesterol is (0.1-5): 0.05-5): 0.1-5): 2-10): 1-5.

In one embodiment, the surfactant is one or more of PEG-40 hydrogenated castor oil, polyvinylpyrrolidone, caprylic/capric triglyceride, tris (laureth-4) phosphate, polyglycerol-10 myristate or isosorbide dimethyl ether; the lecithin is one or more of soybean lecithin, egg yolk lecithin and hydrogenated substance thereof.

In a second aspect of the present invention, there is provided a method for preparing a composition for tightening and lifting skin, comprising the steps of:

s1: mixing and diffusing a retinol component, lecithin, cholesterol and an organic solvent, evaporating to dryness and drying to obtain a first mixture;

s2: adding a peptide component and a surfactant into the first mixture for hydration and diffusion to obtain a second mixture; and homogenizing the second mixture under high pressure by using microjet to obtain the composition for tightening and lifting the skin.

In one embodiment thereof, the organic solvent is absolute ethanol or diethyl ether; the evaporation temperature in the step S1 is 15-30 ℃; and in the step S2, the speed of the high-pressure homogenization of the micro jet is 800-1000r/min, and the pressure is 800-1200bar.

In a third aspect of the invention, the application of the composition for tightening and lifting the skin is provided, and the composition for tightening and lifting the skin is applied to the preparation of skin care products.

In one embodiment, the skin care product is essence which comprises the following components in percentage by mass: 0.5-20% of composition for tightening and lifting skin, 0.1-0.5% of thickening agent, 5-20% of humectant, 0.5-10% of emollient, 0.1-3% of skin conditioner, 0.1-3% of emulsifier, 1-5% of preservative, 0.05-0.2% of essence and the balance of water.

In one embodiment, the thickener is one or a combination of more than two of xanthan gum, carbomer, acrylate or sclerotium rolfsii gum.

In one embodiment, the humectant is one or a combination of two or more of polyethylene glycols, β -glucans, glycerols, polyols, trehalose, allantoin, betaines, glycerol glucosides, hyaluronic acids, or PCA salts.

In one embodiment, the emollient is one or a combination of more than two of squalane, jojoba oil, almond oil, shea butter, tridecyl trimellitate, caprylic/capric triglyceride, isopropyl myristate, myristyl myristate, ethylhexyl palmitate, decyl cocoate, or ethylhexyl stearate, triglyceride, and dimethicone.

In one embodiment, the skin conditioner is a composition comprising one or more of stem extract of DENDROBIUM NOBILE (DENDROBIUM NOBILE), leaf extract of ALOE BARBADENSIS (ALOE BARBADENSIS), root extract of SOPHORA FLAVESCENS (SOPHORA FLAVESCENS), fruit extract of LYCIUM BARBARUM (LYCIUM BARBARUM), acetyl chitosamine, D-panthenol, zingiber officinale extract, or carnosine.

In one embodiment, the emulsifier is one or more of PEG-40 hydrogenated castor oil, octyl dodecanol xyloside, stearyl alcohol polyether-21, sorbitan olive oleate tridecyl alcohol polyether-9.

In one embodiment, the preservative is one or a combination of more than two of parabens, 1, 2-hexanediol, 1, 2-pentanediol, p-hydroxyacetophenone, phenoxyethanol or chlorphenesin.

The present invention is further described below with reference to specific examples so that those skilled in the art can better understand and implement the technical solutions of the present invention, and these examples should not be further construed as limiting the technical solutions.

The essence of examples 1 to 4 and comparative examples 1 to 5 comprises the raw material components in percentage by mass as shown in table 1.

Table 1 raw material components mass percentages

Note: "-" indicates the absence of this component. Comparative example 5 is different from example 1 in that conotoxin peptide and HPR are not prepared into nanoliposomes.

The preparation method of the essence comprises the following steps:

the preparation method of the D1 phase nano liposome comprises the following steps:

adding HPR, lecithin, cholesterol and anhydrous ethanol into round-bottom flask, sufficiently diffusing under ultrasonic condition, evaporating to dryness in rotary evaporator at 15-30 deg.C, and drying in vacuum drying oven to obtain membrane-like capsule wall material containing retinol.

Adding the conotoxin peptide and the caprylic/capric triglyceride into a round-bottom flask for hydration, continuing water bath and ultrasound for diffusion, homogenizing the mixed solution at high pressure by using micro-jet, homogenizing at the speed of 800-1000r/min and under the pressure of 800-1200bar, and finally removing impurities to obtain the nano liposome of the composition for tightening and lifting the skin.

Adding the raw materials of the phase A into water, stirring and mixing uniformly, and heating to 85 ℃ for later use;

mixing the raw materials of phase B, and heating to 85 ℃ for later use;

mixing the C-phase raw materials, and heating to dissolve for later use;

adding the phase B raw material into the phase A raw material for emulsification, homogenizing for 5 minutes under the condition of 8000RPM, adding the phase C, and uniformly mixing for later use;

cooling to 40 deg.C, adding phase D1 and phase D2, supplementing lost water, and homogenizing at 5000RPM for 2 min to obtain essence.

Test example 1 stability test.

The essences prepared in examples 1 to 3 and comparative example 5 were tested for stability under the following conditions, and the results are reported in table 2.

And (3) testing conditions are as follows: and (3) placing the sample in a constant temperature device with 50 ℃, 40 ℃, 25 ℃, 5 ℃, minus 15 ℃ and UV irradiation for stability test, and taking out the sample respectively at 0, 3, 7, 14, 21, 30, 60 and 90 days to observe whether the product has abnormal appearance change phenomena such as obvious color change, delamination, precipitation and the like.

Table 2 stability test results of examples 1 to 3 and comparative example 5

As can be seen from the data in Table 2, examples 1-3 showed color changes in the UV and high temperature environments, but did not change much, and comparative example 5 showed oil bloom and water-oil separation under the UV, 50 deg.C, 40 deg.C and 25 deg.C conditions, and showed water evolution after recovery temperatures of 5 deg.C and-15 deg.C, with a dramatic change in stability. The composition for rapidly tightening and lifting the facial contour can improve the stability of the composition by carrying out nanoliposome coating.

Experimental example 2 irritation test.

The essences prepared in example 1 and comparative example 5 were tested for irritancy under the following conditions, and the results are shown in fig. 1.

And (3) testing conditions are as follows: lactic acid sting positive volunteers were screened for inclusion by the 10% lactic acid sting test: the nasolabial sulcus was smeared with 10% lactic acid at room temperature. The stabbing pain degree was evaluated by 4-point method at 0.5min, 2.5min and 5.0min, respectively. The non-stabbing pain is 0 point, the mild stabbing pain is 1 point, the moderate stabbing pain is 2 points, the severe stabbing pain is 3 points, the time for starting to apply 10% lactic acid on one side is 0min, water is applied on the other side (blank control), and subjects with the pain value of the lactic acid stabbing pain side being more than or equal to 3 points are screened and grouped.

30 lactic acid-sensitive volunteers with 10% concentration were screened, and the sample of example 1 was randomly applied to one cheek and the sample of comparative example 5 was applied to the other cheek, and the application time was measured at 0min, and discomfort reactions such as stinging and itching of the cheeks were subjectively evaluated at 5min, 10min, 20min and 30 min.

As can be seen from figure 1, the number of people who feel uncomfortable after the face on one side is coated with the composition in example 1 is 3 and accounts for 10%, the number of people who feel uncomfortable after the face on one side is 17 and accounts for 57%, the irritation of the face on one side is far less than that of the face on one side in comparative example 5, and the safety of the face on one side is 5.7 times that of the face on one side in comparative example 5.

Test example 3 penetration-promoting efficacy test.

Zebrafish share the same neural mediators and regulatory mechanisms as the human body, and therefore, zebrafish have been widely used in neuroactive drug research and testing. Some components in the cosmetics such as conopeptide, hexapeptide, snake venom-like peptide and the like can effectively block the nerve ending signal conduction of the skin to play the effects of relieving wrinkles and pulling and tightening the skin immediately. Measuring the moving distance of the zebra fish embryo by using instrument recording and software through zebra fish embryo behaviourology, comparing the moving distance changes of the zebra fish embryos of the test object treatment group and the blank control group, and calculating the moving distance shortening rate to evaluate the anti-wrinkle and anti-compaction effects of the raw materials or the products.

Experiments are carried out on the essence prepared in the examples 1-3 and the comparative examples 1-4 according to the following conditions, and if the shortening rate of the movement distance of the zebra fish in the culture solution containing the compound component of the HPR and the conotoxin peptide is higher than that of the HPR or the conotoxin peptide which is singly used, the permeation promoting effect of the HPR on the conotoxin peptide is proved. The experimental groups are shown in table 3, the survival condition of the zebra fish is shown in table 4, and the nerve soothing (tightening and anti-wrinkle) results of the zebra fish are shown in fig. 2.

And (3) testing conditions are as follows:

(1) Sample treatment: homogenizing and mixing the essence of examples 1-3 and the essence of comparative examples 1-4 with water at a ratio of 20g/L at a high speed, performing ultrasonic treatment for 10min, adding fish embryo culture solution to obtain the required concentration, shaking for 30s, centrifuging at 6500r/min for 10min, and collecting the supernatant for testing. The concentration of the test solution of the test object needs to ensure that the death rate of the zebra fish embryos is less than 10 percent (embryos without heartbeat characteristics are defined as death), and 3 concentration groups are set during the test.

(2) The testing steps are as follows: healthy, fertilized 5-day old zebrafish embryos were selected. The test requires a blank control group (fish embryo culture solution), a positive control group (tricaine working solution) and a sample group (test sample). If organic solvent is used for assisting dissolution, the concentration of the solvent in each group of solution needs to be the same. Randomly selecting 16 tail fish embryos, transferring the embryos to a 96-well plate, wherein each well contains one tail fish embryo and 0.2mL of test substance solution, a blank control is a pure zebra fish culture solution, and a positive control is a zebra fish culture solution added with 0.2mL of tricaine working solution. Placing in a thermostat with the temperature of 28 +/-1 ℃ for 2h. Then transferring the zebra fish embryos to a zebra fish motion recorder, and recording the swimming track of each zebra fish embryo in 5 minutes by using infrared tracking software in a dark environment. Replacing 0.18mL of solution in each well with fish embryo culture solution, placing in a thermostat at 28 + -1 ℃ for 24 + -1 h, and counting the survival rate of fish embryos.

(3) Data processing: the pixel intensity per well was read as the distance traveled per fish embryo.

Calculating the movement distance shortening rate: shortening = C-T/C × 100%;

in the formula:

t is the average value of the moving distance of the embryos of the test object treatment group fish;

c, average value of moving distance of fish embryos in the blank control group;

and (4) carrying out double-tail T test on the moving distance of the zebra fish in the test object treatment group and the moving distance of the zebra fish in the blank control group to obtain a P value.

TABLE 3 grouping table of samples

TABLE 4 Zebra fish survival

Example grouping	Survival rate after 24h
		1 (1% conotoxin peptide +1% HPR)	100％
2 (2% conotoxin peptide +1% HPR)	30％
		3 (3% conotoxin peptide +1% HPR)	0％
4 (1% conotoxin peptide +0.5% HPR)	100％

As can be seen from the data in Table 4 and FIG. 2, 1% conotoxin peptide combined with 1% HPR can shorten the distance of zebrafish movement by 35%, which is stronger than-12% of the rate of shortening movement of 1% conotoxin peptide alone and-8% of 1% HPR alone. The 2% conotoxin peptide was compounded with 1% hpr to shorten the zebrafish movement distance by 82%, which is stronger than 47% for the movement distance shortening rate of 2% conotoxin peptide alone and 1% hpr alone, but the survival rate of zebrafish after 24h was only 30%, since the human tolerance was stronger than that of zebrafish, in order to make the data more accurate, the test was carried out to reduce the concentration of example 2 and comparative examples 2, 4 by 50% (i.e. sample group 4), and the results showed that the movement distance of zebrafish after compounding 1% conotoxin peptide and 0.5% hpr was shortened by 18%, again proving that the conotoxin peptide: when the HPR is 2. The shortening rate of 3% conotoxin peptide and 1% HPR after compounding on the movement distance of the zebra fish is 24%, which is lower than the 56% movement shortening rate of 3% conotoxin peptide alone. The test results show that the conotoxin peptide of the invention: when the mass ratio of the HPR to the total mass of the conotoxin is 1.

Test example 4 immediate stress and anti-aging efficacy test.

The essences prepared in example 2 and comparative example 3 were tested under the following conditions, and the test results are shown in tables 5 and 6, the immediate tightening effect is shown in fig. 3, and the long-lasting skin texture improvement is shown in fig. 4.

And (3) testing conditions:

(1) Screening volunteers: selecting 30 healthy volunteers aged over 27 years, with no serious skin disease history, no cosmetic allergy history, eye angle lines, under-eye lines, head raising lines and facial lines at least at two places, and without limitation of gender and skin quality.

(2) And (3) testing: the facial cleansing method comprises the steps that face cleaning is uniformly carried out by using an amino acid cleansing face before samples are used by all volunteers, the facial cleansing face is statically seated in a constant-temperature and constant-humidity efficacy evaluation room for more than 5min, the skin elasticity tester is used for measuring the elasticity and the tightness of the face to obtain D0 data, the Visia7 is used for measuring the facial texture to obtain D0 data, the facial texture is randomly divided into two groups, one group uses the essence of the embodiment 2, the other group uses the essence of the comparative example 3, the instant measurement of the skin elasticity and the tightness is carried out after the facial cleansing face is used for 10min, the facial texture is used once in the morning and at night, the use amounts are the same, and the facial elasticity, the tightness and the texture improvement conditions are measured after 7 days, 14 days and 28 days respectively.

(3) Specification of indexes: r2 value: the higher the reading is, the better the elasticity of the face is; r0 value: compactness, lower readings indicate better facial compactness; f4 value: firmness, lower readings indicate better facial firmness.

(4) Data processing:

average X = ∑ X/n, where X = individual parameter measurement, n = number of valid data

Mean change rate relative to initial value = X _{After T use} -X _{Before T use}

Statistical analysis was performed using SPSS Statistics 25, two-tailed test, test level α =0.05.

And (3) selecting a difference analysis method according to the normal distribution test result of the metering data: if the measured value is normally distributed, performing statistical analysis by adopting a t test method; if the distribution is abnormal, the statistical analysis is carried out by adopting a rank sum test method.

Table 5 face elasticity data

TABLE 6 facial Fine Trench data

The data in table 5 show that the essence prepared in example 2 has a significant effect of instantly pulling and tightening the skin, the elasticity and tightness of the back part after 10min are significantly higher than those of comparative example 3 (only containing conotoxin peptide without HPR) and are about 3 times of those of comparative example 3, which indicates that the effect of instantly tightening and relaxing the nerves of the conotoxin peptide can be significantly improved by containing HPR in the essence.

The data in tables 5, 6 and figure 4 show that the facial elasticity and texture is greatly improved after 7 days of product use, significantly better than the initial elasticity and firmness values, and significantly better than comparative example 3. The fact that the peptide component for inhibiting the acetylcholine and the retinol component are compounded in the essence can obviously improve the wrinkle problem of the skin and achieve the effect of long-acting anti-aging. The data show that the composition for promoting the penetration of the peptide component and improving the efficacy of the peptide component by using the retinol component can achieve the effect of quickly tightening and lifting the facial contour.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims (10)

1. The composition for tightening and lifting skin is characterized by comprising the following components: peptide component (b): the mass ratio of retinol components is (0.1-5) to (0.05-5).

2. The composition for tightening and lifting skin as claimed in claim 1, wherein the mass ratio of the peptide component to the retinoid component is (1-2): 1.

3. The composition for tightening and lifting skin according to claim 1, wherein the peptide component is one or a combination of two or more of acetyl hexapeptide-3, acetyl hexapeptide-8, acetyl hexapeptide-1, acetyl tetrapeptide-5, acetyl tetrapeptide-9, snake venom like peptide, conotoxin peptide and erythroxin peptide.

4. The composition for tightening and lifting skin as claimed in claim 1, wherein the retinol component is one or more of retinol, retinoic acid, hydrogenated retinol, retinol retinoic acid ester, retinol acetate, retinol palmitate, retinol linoleate, retinol propionate, retinol retinoic acid ester or hydroxy pinacolone retinoic acid ester.

5. The firming and lifting skin composition as defined in any one of claims 1 to 4, comprising the following components: peptide component (c): retinol component: surfactant (b): lecithin: the mass ratio of the cholesterol is (0.1-5): (0.05-5): 0.1-5): 2-10): 1-5.

6. The composition for tightening and lifting skin as claimed in claim 5, wherein the surfactant is one or more of PEG-40 hydrogenated castor oil, polyvinylpyrrolidone, caprylic/capric triglyceride, tris (laureth-4) phosphate, polyglycerol-10 myristate and isosorbide dimethyl ether; the lecithin is one or more of soybean lecithin, egg yolk lecithin and hydrogenated substance thereof.

7. A method of preparing the firming and lifting skin composition as defined in any one of claims 1 to 6, comprising the steps of:

s1: mixing and diffusing a retinol component, lecithin, cholesterol and an organic solvent, evaporating to dryness and drying to obtain a first mixture;

s2: adding a peptide component and a surfactant into the first mixture for hydration and diffusion to obtain a second mixture; and homogenizing the second mixture at high pressure by using micro-jet to prepare the composition for tightening and lifting the skin.

8. The method for preparing the composition for tightening and lifting skin as claimed in claim 7, wherein the organic solvent is absolute ethyl alcohol or ethyl ether; the evaporation temperature in the step S1 is 15-30 ℃; the speed of the micro-jet high-pressure homogenization in the step S2 is 800-1000r/min, and the pressure is 800-1200bar.

9. Use of the firming and lifting skin composition according to any one of claims 1 to 6, in the preparation of a skin care product.

10. The application of the composition for tightening and lifting skin as claimed in claim 9, wherein the skin care product is an essence comprising the following components in percentage by mass: 0.5-20% of composition for tightening and lifting skin, 0.1-0.5% of thickening agent, 5-20% of humectant, 0.5-10% of emollient, 0.1-3% of skin conditioner, 0.1-3% of emulsifier, 1-5% of preservative, 0.05-0.2% of essence and the balance of water.

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