CN115969043A - Production method of bovine bone collagen immunoregulation peptide solid beverage - Google Patents
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Abstract
The invention discloses a production method of a bovine bone collagen immunoregulation peptide solid beverage, which comprises the following steps: step one, pretreatment; step two, extracting to obtain a bovine bone collagen extracting solution; step three, performing enzymolysis twice to obtain a bovine bone collagen peptide crude extract; step four, centrifugally filtering to obtain a bovine bone collagen peptide filtrate; step five, purifying to obtain a bovine bone collagen peptide purified solution; step six, concentrating to obtain a bovine bone collagen peptide concentrated solution; seventhly, sterilizing and drying to obtain bovine bone collagen peptide powder; and step eight, mixing and packaging the bovine bone collagen peptide powder, the alpha-amylase inhibitor and the vitamins. The production method of the bovine bone collagen immunoregulation peptide solid beverage has the advantages that the small molecular peptide powder is produced within 24 hours from the treatment of raw materials, the molecular weight is less than 1000Da, the activity of nutrient substances is greatly reserved, the absorption speed is high, the energy consumption is low, the saturation is not easy, the absorption is faster than that of corresponding free amino acid, and the eating is simple and convenient.
Description
Technical Field
The invention belongs to the technical field of food and biology, and particularly relates to a production method of a bovine bone collagen immunoregulation peptide solid beverage.
Background
The peptide is a basic material for forming human body cells, is a nutrient substance between amino acid and protein, has small molecular weight, does not need decomposition or energy consumption, and is 100 percent absorbed by the organism. The functions of various cells and all life behaviors in human bodies, such as growth, development, multiplication, metabolism and the like, can be realized only by peptides, and the peptides influence the synthesis quantity, quality and speed of proteins, control diseases and aging of the human bodies and are key substances for determining the quality of life of the human bodies.
Small peptides currently exist in a variety of forms, and can be classified into plant peptides and animal peptides according to their source, and animal peptides have many advantages over plant peptides. Firstly, the human is an animal, so that the animal peptide is certainly more beneficial to the human from the attribution; and secondly, the animal peptide has more and richer human essential amino acid types than the plant peptide. The animal protein contains 8 kinds of amino acids essential for human body, and is matched with the human nutrition structure; and thirdly, the animal peptide has more targeting property and is more beneficial to the absorption of human bodies. Research shows that the kinds and amount of small molecule active peptides contained in different organs of animal are different, the active peptides contained in heart mainly act on heart, and the active peptides contained in liver mainly act on liver. Meanwhile, different organs of animals selectively gather different trace elements, such as the heart contains more cobalt and zinc, the liver contains more iron, and the bone marrow contains more calcium and magnesium. Therefore, the active peptides and trace elements contained in animal organs are substantially the same as those of human. After the active peptides extracted from different animal organs enter a human body, the active peptides can selectively act on corresponding organs to play the roles of repairing and protecting, and the characteristics and advantages are not possessed by other peptides.
At present, most animal small peptides in the market are mainly bovine bone collagen peptides, but in the prior art, the bovine bone collagen peptides can generate a plurality of unnecessary products in the enzymolysis process, lack suitable protease and have low yield.
Disclosure of Invention
The invention aims to provide a production method of a bovine bone collagen immunoregulation peptide solid beverage, and aims to solve the problem that the yield of bovine bone collagen peptide is low in the enzymolysis process in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a production method of a bovine bone collagen immunoregulation peptide solid beverage comprises the following steps,
s1, pretreatment: selecting a plurality of fresh cattle thigh bones, removing fat and residual minced meat on the surfaces of the cattle bones, cleaning, cutting into blocks, carrying out degreasing treatment, and cleaning with clear water after the treatment is finished;
s2, extracting: crushing cleaned ox bone, adding water, heating and extracting to obtain ox bone collagen extracting solution;
s3, enzymolysis: adding appropriate enzyme into the bovine bone collagen extract for enzymolysis, and performing enzymolysis twice, wherein the enzyme for enzymolysis twice is trypsin and alkaline protease respectively to obtain a crude bovine bone collagen peptide extract;
s4, filtering: centrifuging and filtering the crude bovine bone collagen peptide extract, collecting supernatant, and filtering to obtain bovine bone collagen peptide filtrate;
s5, purification: enabling the bovine bone collagen peptide filtrate to pass through a pre-filled macroporous adsorption resin chromatographic column at a proper flow rate, eluting 1-4 beads by using clear water for removing impurities after sample loading is finished, eluting by using 55-80% ethanol water solution, collecting eluent, putting activated carbon into the eluent, heating for a certain time, and filtering while the solution is hot to prepare a bovine bone collagen peptide purified solution;
s6, concentrating: concentrating the purified bovine bone collagen peptide solution in multiple effects, concentrating until no ethanol smell exists, and recovering ethanol to obtain concentrated bovine bone collagen peptide solution;
s7, sterilizing and drying: spray drying the concentrated solution of bovine bone collagen peptide, and sterilizing to obtain bovine bone collagen peptide powder;
s8, mixing and packaging: mixing bovine bone collagen peptide powder, alpha-amylase inhibitor and vitamins, micronizing, and packaging.
Further, the cattle bone degreasing step in the step S1 is as follows: putting a proper amount of clear water into the pot, adding a proper amount of sodium citrate into the ox bone, boiling the ox bone with water over strong fire for 0.3 to 0.7 hour, skimming off the floating oil on the surface, and continuing to boil with water until the floating oil is completely skimmed off.
Further, the heating and extracting conditions in the step S2 are as follows: material-liquid ratio of 1: 6-1, 10kg/L, the extraction temperature is 80-90 ℃, and the extraction time is 5-10 h.
Preferably, the ratio of the material to the liquid is 1: 6. 1: 7. 1: 8. 1:9 and 1:10 at 80 deg.C, 85 deg.C and 90 deg.C for 5h, 6h, 7h, 8h, 9h or 10h.
Further, in the step S3, the addition amount of the trypsin and the alkaline protease is 2-8 g/100g, the enzymolysis temperature is 40-55 ℃, and the enzymolysis time is 3-5 hours.
Further, in the step S4, the centrifugal speed is 4000-5000 r/min, and the centrifugal time is 5-10 min.
Preferably, the centrifugation speed is any one of 4000r/min, 4500r/min and 5000r/min, and the centrifugation time is any one of 5min, 6min, 7min, 8min, 9min or 10min.
Further, in the step S5, the heating temperature of the activated carbon is 90-100 ℃, and the heating time is 15-30 min.
Preferably, the activated carbon is heated at any one of 90 ℃, 95 ℃ and 100 ℃ for any one of 15min, 20min and 30min.
Further, the α -amylase inhibitor in step S8 is any one of hairm produced by the streptomyces YM-25 strain, paim produced by the streptomyces S scorhorruchii strain, and trestatin produced by the streptomyces S dimorph genes strain, and is added in an amount of 0.01 to 0.05% by weight.
Preferably, the amount of the alpha-amylase inhibitor added is 0.01%, 0.02%, 0.03%, 0.04%, or 0.05%.
Further, the type of the vitamin in the step S8 is one or a combination of several of vitamin B1, vitamin B2, vitamin B6, and vitamin B12, and the addition amount is 0.1 to 0.5% by weight.
Preferably, the vitamin is added in an amount of 0.1%, 0.2%, 0.3%, 0.4% or 0.5%.
Further, the superfine grinding in the step S8 has a mesh size of 100-120 meshes.
Preferably, the superfine powder has 100 meshes, 105 meshes, 110 meshes, 115 meshes or 120 meshes.
Further, the sterilization mode in the step S7 is a hot-pressing sterilization method, the temperature is 115 to 120 ℃, and the time is 15 to 30min.
Preferably, the temperature of autoclaving is 115 ℃, 116 ℃, 117 ℃, 118 ℃, 119 ℃ or 120 ℃; the time is 15min, 20min, 25min or 30min.
Compared with the prior art, the production method of the bovine bone collagen immunoregulation peptide solid beverage has the beneficial effects that:
1) The scientific proportioning is as follows: the calf thigh bone of Qinchuan cattle is selected strictly, the excellent quality of the raw materials is ensured from the source, the cold chain transportation is carried out in the whole process, the time from the beef bone raw material to the production of the collagen peptide powder is not more than 24 hours, the nutrient substances and the activity are greatly reserved, and the nutrition is more balanced and easy to absorb;
2) High absorption rate and good activity: advanced biological technologies such as degreasing, desalting, twice enzymolysis, filtering and the like are adopted in the purification process, the molecular weight is small, the protease can be directly absorbed by cells, secondary decomposition or synthesis is not needed, the yield of the protease is high, and the bioavailability is high;
3) No additive and no auxiliary material: the peptide content is more than or equal to 98 percent, the whole bone collagen powder is compounded with vitamins, and the bone collagen powder is free of any pigment, additive, preservative and auxiliary material and can be safely taken for a long time;
4) Good taste, convenient eating and good weight-reducing and losing efficacy: has no peculiar smell and fishy smell, is convenient to carry, easy to brew and quick to dissolve, and can be taken together with honey, milk and the like.
Drawings
FIG. 1 is a process flow chart of a method for producing a bovine bone collagen immunomodulatory peptide solid beverage according to an embodiment of the invention.
Detailed Description
The technical scheme of the invention is further explained by the specific implementation mode in combination with the attached drawings.
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1, fig. 1 is a process flow chart of a method for producing a bovine bone collagen immunomodulatory peptide solid beverage according to an embodiment of the present invention.
Example one
In the embodiment, the production method of the bovine bone collagen immunoregulation peptide solid beverage comprises the following steps:
step one, selection and pretreatment of cattle bones: selecting about 1 kg of fresh thigh bones of slaughtered Qinchuan cattle, removing fat and residual minced meat on the surface of the cattle bones, cleaning, cutting into blocks, boiling water in a pot, adding a proper amount of sodium citrate, putting the bones over the water, boiling the bones over big fire for about half an hour, skimming off superficial oil, and continuing to boil the bones until all the superficial oil is skimmed off; cleaning with clear water after the treatment is finished;
step two, extraction: crushing the bovine bone obtained in the first step, and adding 6L of water for extraction at the extraction temperature of 80 ℃ for 5 hours to obtain a bovine bone collagen extracting solution; it should be noted that: the extraction temperature can also be 85 ℃ or 90 ℃; the extraction time can be any one of 6h, 7h, 8h, 9h or 10 h;
step three, enzymolysis: adjusting the pH value of the bovine bone collagen protein extracting solution in the second step to 9.1, adding 10 g of trypsin and 10 g of alkaline protease for enzymolysis, wherein the enzymolysis temperature is 40 ℃, and the enzymolysis time is 3 hours, so as to obtain a bovine bone collagen peptide crude extracting solution; it should be noted that: the enzymolysis temperature can also be 45 ℃ or 50 ℃, and the enzymolysis time can also be 4 hours or 5 hours;
step four, filtering: centrifuging and filtering the bovine bone collagen peptide crude extract obtained in the step three, wherein the centrifugation speed is 4000r/min, and the centrifugation time is 5min; collecting supernatant, and filtering to obtain bovine bone collagen peptide filtrate. It should be noted that: the centrifugation speed can also be 4500r/min or 5000r/min, and the centrifugation time can also be any one of 6min, 7min, 8min, 9min or 10min;
step five, purification: enabling the bovine bone collagen peptide filtrate to pass through a pre-filled macroporous adsorption resin chromatographic column at a proper flow rate, eluting 2 bead volumes by using clear water for removing impurities after sample loading is finished, eluting by using 55% ethanol water solution, collecting eluent, putting activated carbon into the eluent, heating at 90 ℃ for 15min, and filtering while the solution is hot to prepare a bovine bone collagen peptide purified solution; it should be noted that: the heating temperature of the activated carbon can also be 95 ℃ or 100 ℃, and the heating time can also be 20min or 30min;
step six, concentration: carrying out multiple-effect concentration on the purified bovine bone collagen peptide liquid obtained in the step five until the solution has no ethanol smell, and recovering ethanol to obtain 0.8L of bovine bone collagen peptide concentrated liquid;
step seven, sterilizing and drying: spray drying and sterilizing the bovine bone collagen peptide concentrated solution prepared in the sixth step to prepare 120g of bovine bone collagen peptide powder; wherein the sterilization mode adopts autoclave sterilization, the autoclave sterilization temperature is 115 ℃, the sterilization time is 15min, and the following are mentioned: the autoclave sterilization temperature can also be 116 deg.C, 117 deg.C, 118 deg.C, 119 deg.C or 120 deg.C, and the sterilization time can also be 20min, 25min or 30min.
Step eight, mixing and packaging: mixing the bovine bone collagen peptide powder prepared in the seventh step with 0.01 percent of alpha-amylase inhibitor trestatin and 0.1 percent of vitamin B1, carrying out superfine grinding and packaging to obtain the bovine bone collagen peptide powder. It should be noted that: the addition amount of alpha-amylase inhibitor trestatin can be 0.02%, 0.03%, 0.04% or 0.05%, the addition amount of vitamin B1 can be 0.2%, 0.3%, 0.4% or 0.5%, and the superfine grinding mesh number is any one of 100 meshes, 105 meshes, 110 meshes, 115 meshes or 120 meshes.
Example two
In this embodiment, a method for producing a bovine bone collagen immunoregulatory peptide solid beverage comprises the following steps:
step one, selection and pretreatment of bovine bones: selecting about 1 kg of fresh thigh bones of slaughtered Qinchuan cattle, removing fat and residual minced meat on the surface of the cattle bones, cleaning, cutting into blocks, boiling water in a pot, adding a proper amount of sodium citrate, putting the bones over the water, boiling the bones over big fire for about half an hour, skimming off superficial oil, and continuing to boil the bones until all the superficial oil is skimmed off; cleaning with clear water after the treatment is finished;
step two, extraction: crushing the bovine bone obtained in the first step, and adding 7L of water for extraction at 85 ℃ for 7 hours to obtain a bovine bone collagen extracting solution; it should be noted that: the extraction temperature can also be 80 ℃ or 90 ℃; the extraction time can be any one of 5h, 6h, 8h, 9h or 10 h;
step three, enzymolysis: adjusting the pH value of the bovine bone collagen protein extracting solution in the second step to 9.2, adding 15 g of trypsin and 15 g of alkaline protease for enzymolysis, wherein the enzymolysis temperature is 45 ℃, and the enzymolysis time is 4 hours, so as to obtain a crude bovine bone collagen peptide extracting solution; it should be noted that: the enzymolysis temperature can also be 40 ℃ or 50 ℃, and the enzymolysis time can also be 3 hours or 5 hours;
step four, filtering: centrifuging and filtering the bovine bone collagen peptide crude extract obtained in the third step at the speed of 4500r/min for 8min; collecting supernatant, and filtering to obtain bovine bone collagen peptide filtrate. It should be noted that: the centrifugation speed can be 4000r/min or 5000r/min, and the centrifugation time can be any one of 5min, 6min, 7min, 9min or 10min;
step five, purification: enabling the bovine bone collagen peptide filtrate to pass through a pre-filled macroporous adsorption resin chromatographic column at a proper flow rate, eluting 2 bead volumes by using clear water for removing impurities after sample loading is finished, eluting by using a 60% ethanol water solution, collecting eluent, putting activated carbon into the eluent, heating at the temperature of 95 ℃ for 20min, and filtering while hot to obtain a bovine bone collagen peptide purified solution; it should be noted that: the heating temperature of the activated carbon can be 90 ℃ or 100 ℃, and the heating time can be 15min or 30min;
step six, concentration: carrying out multiple-effect concentration on the purified bovine bone collagen peptide liquid obtained in the step five until the solution has no ethanol smell, and recovering ethanol to obtain 0.8L of bovine bone collagen peptide concentrated liquid;
step seven, sterilizing and drying: spray drying and sterilizing the bovine bone collagen peptide concentrated solution prepared in the sixth step to prepare 120g of bovine bone collagen peptide powder; wherein the sterilization mode adopts autoclave sterilization, the autoclave sterilization temperature is 118 ℃, the sterilization time is 20min, and the following are mentioned: the autoclave sterilization temperature can also be 115 deg.C, 116 deg.C, 117 deg.C, 119 deg.C or 120 deg.C, and the sterilization time can also be 15min, 20min or 30min.
Step eight, mixing and packaging: mixing the bovine bone collagen peptide powder prepared in the seventh step with 0.02% of alpha-amylase inhibitor paim and 0.2% of vitamin B1, carrying out superfine grinding and packaging to obtain the bovine bone collagen peptide powder. It should be noted that: the addition amount of the alpha-amylase inhibitor paim can be 0.01%, 0.03%, 0.04% or 0.05%, the addition amount of the vitamin B1 can be 0.1%, 0.3%, 0.4% or 0.5%, and the superfine grinding mesh number is any one of 100 meshes, 105 meshes, 110 meshes, 115 meshes or 120 meshes.
EXAMPLE III
In this embodiment, a method for producing a bovine bone collagen immunoregulatory peptide solid beverage comprises the following steps:
step one, selection and pretreatment of bovine bones: selecting about 1 kg of fresh thighbone of a slaughtered Qinchuan cattle, removing fat and residual minced meat on the surface of the cattle bone, cleaning, cutting into blocks, boiling water in a pot, adding a proper amount of sodium citrate, enabling the water to submerge the bone, putting the bone on strong fire, boiling for about half an hour, skimming off superficial floating oil, and continuing to boil with the water until all the floating oil is skimmed off; cleaning with clear water after the treatment is finished;
step two, extraction: crushing the bovine bone obtained in the first step, and adding 8L of water for extraction at 90 ℃ for 10 hours to obtain a bovine bone collagen extracting solution; it should be noted that: the extraction temperature can be 80 ℃ or 85 ℃; the extraction time can be any one of 5h, 6h, 7h, 8h or 9 h;
step three, enzymolysis: adjusting the pH value of the bovine bone collagen extract liquid obtained in the step two to 9.2, adding 18 g of trypsin and 18 g of alkaline protease for enzymolysis, wherein the enzymolysis temperature is 50 ℃, and the enzymolysis time is 5 hours, so as to obtain a crude bovine bone collagen peptide extract liquid; it should be noted that: the enzymolysis temperature can also be 40 ℃ or 45 ℃, and the enzymolysis time can also be 3 hours or 4 hours;
step four, filtering: centrifuging and filtering the bovine bone collagen peptide crude extract obtained in the third step at the speed of 5000r/min for 10min; collecting supernatant, and filtering to obtain bovine bone collagen peptide filtrate. It should be noted that: the centrifugation speed can be 4000r/min or 4500r/min, and the centrifugation time can be any one of 5min, 6min, 7min, 8min or 9 min;
step five, purification: enabling the bovine bone collagen peptide filtrate to pass through a pre-filled macroporous adsorption resin chromatographic column at a proper flow rate, eluting 2 bead volumes by using clear water for removing impurities after sample loading is finished, eluting by using a 60% ethanol water solution, collecting eluent, putting activated carbon into the eluent, heating at 100 ℃ for 30min, and filtering while the solution is hot to prepare a bovine bone collagen peptide purified solution; it should be noted that: the heating temperature of the activated carbon can be 90 ℃ or 95 ℃, and the heating time can be 15min or 20min;
step six, concentration: carrying out multiple-effect concentration on the bovine bone collagen peptide purified solution obtained in the fifth step until the solution has no ethanol smell, and recovering ethanol to obtain 0.8L of bovine bone collagen peptide concentrated solution;
step seven, sterilizing and drying: spray drying and sterilizing the bovine bone collagen peptide concentrated solution prepared in the sixth step to prepare 120g of bovine bone collagen peptide powder; wherein, the sterilization mode adopts the hot-pressing sterilization, the temperature of the hot-pressing sterilization is 120 ℃, the sterilization time is 30min, and the following steps are required: the autoclave sterilization temperature can also be 115 deg.C, 116 deg.C, 117 deg.C, 118 deg.C or 119 deg.C, and the sterilization time can also be 15min, 20min or 25min.
Step eight, mixing and packaging: mixing the bovine bone collagen peptide powder prepared in the seventh step with 0.05% of alpha-amylase inhibitor hairm and 0.5% of vitamin B1, carrying out superfine grinding and packaging to obtain the bovine bone collagen peptide powder. It should be noted that: the addition amount of alpha-amylase inhibitor hairm can be 0.01%, 0.02%, 0.03% or 0.04%, the addition amount of vitamin B1 can be 0.1%, 0.2%, 0.3% or 0.4%, and the mesh number of superfine grinding is any one of 100 meshes, 105 meshes, 110 meshes, 115 meshes or 120 meshes.
Test example:
45 subjects (36 men and 9 women) were selected as subjects, and the subjects were aged at the average age (34.2. + -. 10.9) and the body mass (81.09. + -. 15.84) kg, wherein 34 human Body Mass Index (BMI) was judged to be overweight (BMI of 24.0 to 27.9kg/m 2) and 11 subjects were obese (BMI of 28.0kg/m 2).
The whole weight-losing experimental period is one month. Testers drink 1 package (6 g) by brewing with 200ml of warm water in the morning and evening respectively, three meals a day are normally carried out, rice flour and other high-starch foods are not limited to be eaten, the weight loss success rate is 50.0% after one month, and an obvious early weight loss effect is generated. 45 testers continue to take the medicine for 1 month, and the weight loss success rate reaches 80.0 percent.
The foregoing embodiments are merely illustrative of the principles and features of this invention, which is not limited to the above-described embodiments, but is capable of various modifications and changes without departing from the spirit and scope of the invention, which are intended to be within the scope of the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. A production method of bovine bone collagen immunoregulation peptide solid beverage is characterized by comprising the following steps,
s1, pretreatment: selecting a plurality of fresh cattle thigh bones, removing fat and residual minced meat on the surface of the cattle bones, cleaning, cutting into blocks, carrying out degreasing treatment, and cleaning with clear water after the treatment is finished;
s2, extracting: crushing cleaned ox bone, adding water, heating and extracting to obtain ox bone collagen extracting solution;
s3, enzymolysis: adding appropriate enzyme into the bovine bone collagen extract for enzymolysis, and performing enzymolysis twice, wherein the enzyme for enzymolysis twice is trypsin and alkaline protease respectively to obtain a crude bovine bone collagen peptide extract;
s4, filtering: centrifuging and filtering the crude bovine bone collagen peptide extract, collecting supernatant, and filtering to obtain a bovine bone collagen peptide filtrate;
s5, purification: enabling the bovine bone collagen peptide filtrate to pass through a pre-filled macroporous adsorption resin chromatographic column at a proper flow rate, eluting 1-4 beads by using clear water for removing impurities after sample loading is finished, eluting by using 55-80% ethanol water solution, collecting eluent, putting activated carbon into the eluent, heating for a certain time, and filtering while the solution is hot to prepare a bovine bone collagen peptide purified solution;
s6, concentrating: carrying out multiple-effect concentration on the bovine bone collagen peptide purified solution until the solution has no ethanol smell, and recovering ethanol to prepare a bovine bone collagen peptide concentrated solution;
s7, sterilizing and drying: spray drying the concentrated solution of bovine bone collagen peptide, and sterilizing to obtain bovine bone collagen peptide powder;
s8, mixing and packaging: mixing bovine bone collagen peptide powder, alpha-amylase inhibitor and vitamins, micronizing, and packaging.
2. The method for producing bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein the step of defatting bovine bone in step S1 comprises: putting a proper amount of clear water into the pot, adding a proper amount of sodium citrate into the beef bones, adding water to submerge the bones, boiling the beef bones with strong fire for 0.3 to 0.7 hour, skimming the floating oil on the surface, and continuing to boil the beef bones with water until the floating oil is completely skimmed.
3. The method for producing the bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein the heating and extracting conditions in step S2 are as follows: the material-liquid ratio is 1: 6-1, 10kg/L, the extraction temperature is 80-90 ℃, and the extraction time is 5-10 h.
4. The production method of the bovine bone collagen immunoregulation peptide solid beverage according to claim 1, wherein in the step S3, the addition amount of trypsin and alkaline protease is 2-8 g/100g, the enzymolysis temperature is 40-55 ℃, and the enzymolysis time is 3-5 h.
5. The method for producing the bovine bone collagen immune modulatory peptide solid beverage according to claim 1, wherein the centrifugation speed in step S4 is 4000-5000 r/min and the centrifugation time is 5-10 min.
6. The method for producing the bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein the heating temperature of the activated carbon in step S5 is 90-100 ℃ and the heating time is 15-30 min.
7. The method for producing a bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein in step S8 the alpha-amylase inhibitor is any one of hairm produced by streptomyces YM-25 strain, paim produced by streptomyces S. Coprochui strain, and trestatin produced by streptomyces S. Dimorphyrins strain, and the amount of addition is 0.01 to 0.05% by weight.
8. The method for producing bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein the type of vitamin in step S8 is one or a combination of several of vitamin B1, vitamin B2, vitamin B6 and vitamin B12, and the addition amount is 0.1-0.5% by weight.
9. The method for producing the bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein the mesh size of the superfine grinding in step S8 is 100-120 meshes.
10. The method for producing the bovine bone collagen immunoregulatory peptide solid beverage according to claim 1, wherein the sterilization mode in step S7 is autoclave sterilization at 115-120 ℃ for 15-30 min.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11346717A (en) * | 1998-06-15 | 1999-12-21 | Sennosuke Tokumaru | Diet health food |
| CN111893156A (en) * | 2020-07-31 | 2020-11-06 | 青海瑞肽生物科技有限公司 | Preparation method of refined yak bone collagen peptide |
| CN114703245A (en) * | 2022-04-14 | 2022-07-05 | 甘肃中肽生物科技有限公司 | Preparation process of bovine bone protein peptide chelated zinc |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11346717A (en) * | 1998-06-15 | 1999-12-21 | Sennosuke Tokumaru | Diet health food |
| CN111893156A (en) * | 2020-07-31 | 2020-11-06 | 青海瑞肽生物科技有限公司 | Preparation method of refined yak bone collagen peptide |
| CN114703245A (en) * | 2022-04-14 | 2022-07-05 | 甘肃中肽生物科技有限公司 | Preparation process of bovine bone protein peptide chelated zinc |
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