CN115944685A

CN115944685A - Traditional Chinese medicine composition beneficial to maintaining blood sugar health level and preparation method and application thereof

Info

Publication number: CN115944685A
Application number: CN202211734116.9A
Authority: CN
Inventors: 潘声旺; 毛勇坡; 冯彬彬; 张建海
Original assignee: Chengdu University
Current assignee: Chengdu University
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-04-11

Abstract

The invention discloses a traditional Chinese medicine composition beneficial to maintaining blood sugar health level and a preparation method and application thereof, and relates to the technical field of medicinal food preparation. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-20 parts of codonopsis pilosula, 15-25 parts of rhizoma polygonati, 15-25 parts of radix puerariae, 15-25 parts of Chinese yam and 6-15 parts of pseudo-ginseng. The medicinal materials can exert the drug effect synergistically, effectively relieve the blood sugar level of diabetic mice, improve the abnormal glucose tolerance, relieve the lipid metabolism disorder and repair damaged liver and pancreatic tissues, thereby reducing the insulin resistance level and improving the insulin content. The invention solves the problem that the existing oral medicine for treating diabetes has adverse reaction after being taken for a long time.

Description

Traditional Chinese medicine composition beneficial to maintaining blood sugar health level and preparation method and application thereof

Technical Field

The invention relates to the technical field of medicinal food preparation, in particular to a traditional Chinese medicine composition for maintaining blood sugar health level and a preparation method and application thereof.

Background

Type 2 diabetes (T2 DM) is a metabolic disease of diabetes, has a very high incidence and many complications, and has become one of the serious health risks in the world. The pathogenesis of T2DM is complex, and the organism in a hyperglycemia state for a long time can not only cause pathological changes such as abnormal glycolipid metabolism and the like, but also cause pathological changes of tissues and organs such as liver, kidney and the like, and can also cause organ failure and even death in severe cases. At present, the common method for clinically treating diabetes is to take insulin, hypoglycemic drugs and the like, and adverse reactions with different degrees can be generated after long-term taking. Therefore, the search for safe and efficient medicaments for treating T2DM is one of the important tasks in the medical field, and the traditional Chinese medicine is rich in active substances with the blood sugar reducing function, such as polysaccharides, flavonoids, alkaloids, saponins and the like, and has a good blood sugar reducing effect. Therefore, the intervention of the T2DM through the reasonable compatibility of the traditional Chinese medicines is a method for treating and preventing diabetes.

Disclosure of Invention

In order to solve the technical problems, the invention aims to provide a traditional Chinese medicine composition which is beneficial to maintaining the healthy level of blood sugar and a preparation method and application thereof, so as to solve the problem that the existing oral medicine for treating diabetes has adverse reactions after being taken for a long time.

The technical scheme for solving the technical problems is as follows: the traditional Chinese medicine composition for maintaining the healthy level of blood sugar is provided, and comprises the following raw materials in parts by weight: 10-20 parts of codonopsis pilosula, 15-25 parts of rhizoma polygonati, 15-25 parts of radix puerariae, 15-25 parts of Chinese yam and 6-15 parts of pseudo-ginseng.

The invention has the beneficial effects that: the traditional Chinese medicine considers that the diabetes belongs to the category of diabetes, namely, the diabetes is manifested as the disease with yin deficiency as the principal and dryness-heat as the secondary, so the clinical application should take yin nourishing, heat clearing, qi tonifying and body fluid production as the treatment principle. Meanwhile, the raw materials are all traditional Chinese medicines which can be used for health-care food, and the variety is rich in China and the cultivation range is wide.

The active ingredients which play a role in the invention are mainly as follows: beta-sitosterol of kudzuvine root, manyflower solomonseal rhizome and sanchi, stigmasterol of yam, dangshen and sanchi, liquiritigenin of manyflower solomonseal rhizome and sanchi, 3' -methoxy daidzein in the kudzuvine root and the manyflower solomonseal rhizome, quercetin which is a special component of sanchi, luteolin which is a special component of the dangshen and isoflavone, and the components have synergistic action: the quercetin has good anti-inflammatory effect, and can effectively improve the liver damage and fibrosis lesion of T2DM mice; the beta-sitosterol and stigmasterol have the functions of reducing blood fat, improving fatty liver and abnormal metabolism, protecting beta cell function to a certain extent and achieving the purpose of relieving insulin resistance; luteolin and liquiritigenin can activate NRF pathway and act on Nrf2 protein on the pathway, thereby improving superoxide dismutase level and relieving oxidative stress injury of myocardial cells after high-sugar stimulation; isoflavone can activate AMP-activated protein kinase in AMPK pathway, and promote glucose uptake; the panax notoginseng has anti-inflammatory effect, and improves the liver damage and fibrosis lesion of diabetic mice; the kudzu root and the rhizoma polygonati have the function of protecting beta cells; the rhizoma Dioscoreae polysaccharide and radix Codonopsis polysaccharide can enhance immunity, and have effects of lowering blood sugar and blood lipid; the above Chinese medicinal materials all have antioxidant effect, and can be used for the synergistic treatment of diabetes.

On the basis of the technical scheme, the invention can be further improved as follows:

further, the composition comprises the following raw materials in parts by weight: 15 parts of codonopsis pilosula, 20 parts of rhizoma polygonati, 20 parts of radix puerariae, 20 parts of Chinese yam and 10 parts of pseudo-ginseng.

The beneficial effect of adopting the further technical scheme is as follows:

the invention also provides a preparation method of the traditional Chinese medicine composition for maintaining the healthy level of blood sugar, which comprises the following steps:

(1) Mixing radix Codonopsis, rhizoma Polygonati, radix Puerariae, rhizoma Dioscoreae and Notoginseng radix, removing impurities, pulverizing, and sieving to obtain Chinese medicinal powder;

(2) Adding purified water into the Chinese medicinal powder prepared in step (1), boiling, decocting with slow fire for 45-60min, and filtering to obtain primary filter residue to obtain primary filtrate;

(3) Adding purified water into the primary filter residue obtained in the step (2), boiling the water, decocting for 45-60min with slow fire, and filtering to obtain a secondary filtrate;

(4) Mixing the primary filtrate obtained in the step (2) and the secondary filtrate obtained in the step (3), filtering, standing for 8-12h, concentrating the obtained supernatant under reduced pressure, and drying to obtain the traditional Chinese medicine composition which is beneficial to maintaining the healthy level of blood sugar.

Further, in the step (1), sieving by a sieve of 20-50 meshes.

Further, in the step (1), the mixture is sieved by a 30-mesh sieve.

Further, in the step (2), the volume ratio of the traditional Chinese medicine powder to the purified water is 1:7-10.

Further, in the step (2), the volume ratio of the traditional Chinese medicine powder to the purified water is 1:8.

further, in the step (3), the volume ratio of the filter residue to the purified water is 1:3-5.

Further, in the step (3), the volume ratio of the filter residue to the purified water is 1:4.

further, in steps (2) - (3), 5-7 layers of gauze are adopted for filtration.

Further, in steps (2) to (3), 6 layers of gauze were used for filtration.

Further, in the step (4), the mixture is concentrated under reduced pressure at 50 to 80 ℃ until the relative density is 1.1 to 1.3.

Further, in the step (4), the mixture is concentrated under reduced pressure at 50 to 80 ℃ until the relative density becomes 1.2.

Further, in the step (4), vacuum drying or spray drying is adopted.

The invention also provides application of the traditional Chinese medicine composition in preparing health-care food which is beneficial to maintaining the health level of blood sugar.

The invention also provides a health food beneficial to maintaining the healthy level of blood sugar, which comprises the traditional Chinese medicine composition and the grain powder.

Further, the cereal powder is buckwheat flour and/or coix seed flour.

Further, the health food comprises tablets, granules or capsules.

The invention has the following beneficial effects:

1. the medicinal materials can exert the drug effect synergistically, effectively relieve the blood sugar level of diabetic mice, improve the abnormal glucose tolerance, relieve the lipid metabolism disorder and repair the damaged liver and pancreatic tissues, thereby reducing the insulin resistance level and improving the insulin content.

2. The traditional Chinese medicine composition can inhibit insulin resistance of a T2DM mouse model, improve abnormal glucose tolerance and blood lipid level of a diabetic mouse, and prevent diabetic complications; meanwhile, the components are reasonably compatible to achieve the effect of coordination and synergism, thereby remarkably increasing the secretion of insulin and achieving the effect of maintaining the normal level of blood sugar.

3. The traditional Chinese medicine composition prepared by the invention can be combined with cereal powder to develop health-care food which is beneficial to maintaining the health level of blood sugar, so that the related industrial chain is enriched and the economic benefit is increased.

Drawings

FIG. 1 is a graph showing the effect of the Chinese medicinal composition prepared in example 1 on the quality of diabetic mice;

FIG. 2 is a graph showing the effect of the Chinese medicinal composition prepared in example 1 on blood glucose concentration in diabetic mice;

FIG. 3 is a graph showing the effect of the Chinese medicinal composition prepared in example 1 on glucose tolerance in diabetic mice;

FIG. 4 shows the effect of the Chinese medicinal composition prepared in example 1 on insulin levels in diabetic mice;

FIG. 5 shows the effect of the Chinese medicinal composition prepared in example 1 on insulin resistance of diabetic mice.

Detailed Description

The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

Example 1:

a traditional Chinese medicine composition helpful for maintaining healthy blood sugar level comprises the following raw materials in parts by weight: 15 parts of codonopsis pilosula, 20 parts of sealwort, 20 parts of kudzu root, 20 parts of Chinese yam and 10 parts of pseudo-ginseng.

A traditional Chinese medicine composition for helping to maintain healthy blood sugar level is prepared by the following steps:

(1) Mixing radix Codonopsis, rhizoma Polygonati, radix Puerariae, rhizoma Dioscoreae and Notoginseng radix, removing impurities, pulverizing, and sieving with 30 mesh sieve to obtain Chinese medicinal powder;

(2) Adding 8 times of purified water into the traditional Chinese medicine powder prepared in the step (1), boiling the water, decocting for 50min with slow fire, and filtering by adopting 6 layers of gauze to obtain primary filter residue and prepare primary filtrate;

(3) Adding purified water with the volume 4 times that of the primary filter residue obtained in the step (2), boiling the water, decocting for 50min with slow fire, and filtering by adopting gauze with 6 layers to obtain secondary filtrate;

(4) Mixing the primary filtrate obtained in the step (2) and the secondary filtrate obtained in the step (3), filtering, standing for 10h, placing the obtained supernatant into a rotary evaporator, concentrating under reduced pressure at 60 ℃ until the relative density is 1.2, and performing vacuum drying or spray drying to obtain the traditional Chinese medicine composition which is beneficial to maintaining the healthy level of blood sugar.

A health food for helping to maintain healthy blood sugar level is prepared by the following steps:

mixing the above Chinese medicinal composition with buckwheat flour, and making into tablet.

Example 2:

a traditional Chinese medicine composition helpful for maintaining healthy blood sugar level comprises the following raw materials in parts by weight: 10 parts of codonopsis pilosula, 15 parts of rhizoma polygonati, 15 parts of radix puerariae, 15 parts of Chinese yam and 6 parts of pseudo-ginseng.

(1) Mixing radix Codonopsis, rhizoma Polygonati, radix Puerariae, rhizoma Dioscoreae and Notoginseng radix, removing impurities, pulverizing, and sieving with 20 mesh sieve to obtain Chinese medicinal powder;

(2) Adding 7 times of purified water into the traditional Chinese medicine powder prepared in the step (1), boiling the water, decocting for 45min with slow fire, and filtering with 5 layers of gauze to obtain primary filter residue and obtain primary filtrate;

(3) Adding purified water with the volume of 3 times of that of the primary filter residue obtained in the step (2), boiling the water, decocting for 45min with slow fire, and filtering by using 5-7 layers of gauze to obtain secondary filtrate;

(4) Mixing the primary filtrate obtained in the step (2) and the secondary filtrate obtained in the step (3), filtering, standing for 8h, placing the obtained supernatant in a rotary evaporator, concentrating under reduced pressure at 50 ℃ until the relative density is 1.1, and performing vacuum drying or spray drying to obtain the traditional Chinese medicine composition which is helpful for maintaining the healthy level of blood sugar.

mixing the above Chinese medicinal composition with buckwheat flour, and making into granule.

Example 3:

a traditional Chinese medicine composition helpful for maintaining blood sugar health level comprises the following raw materials in parts by weight: 20 parts of codonopsis pilosula, 25 parts of rhizoma polygonati, 25 parts of radix puerariae, 25 parts of Chinese yam and 15 parts of pseudo-ginseng.

(1) Mixing radix Codonopsis, rhizoma Polygonati, radix Puerariae, rhizoma Dioscoreae and Notoginseng radix, removing impurities, pulverizing, and sieving with 50 mesh sieve to obtain Chinese medicinal powder;

(2) Adding 10 times of purified water into the traditional Chinese medicine powder prepared in the step (1), boiling the water, decocting for 60min with slow fire, and filtering by using 7 layers of gauze to obtain primary filter residue and prepare primary filtrate;

(3) Adding purified water with the volume 5 times that of the primary filter residue obtained in the step (2), boiling the water, decocting for 60min with slow fire, and filtering by using 7 layers of gauze to obtain secondary filtrate;

(4) Mixing the primary filtrate obtained in the step (2) and the secondary filtrate obtained in the step (3), filtering, standing for 12h, placing the obtained supernatant in a rotary evaporator, concentrating under reduced pressure at 80 ℃ until the relative density is 1.3, and performing vacuum drying or spray drying to obtain the traditional Chinese medicine composition which is helpful for maintaining the healthy level of blood sugar.

A health food for helping to maintain healthy blood sugar level is prepared by the following steps: mixing the above Chinese medicinal composition with buckwheat flour, and making into capsule.

Test examples

1. Pharmacological experiments

1. Materials and instrumentation

Metformin hydrochloride tablets, batch number: 28800803, tianjin Pacific pharmaceuticals, inc.; high-fat high-sugar feed, batch number: 202111213, streptozotocin (STZ), batch No.: 2021092706, bio-technologies ltd, boai harbor, beijing; serum Total Cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C) kit, lot No.: 20220118, mouse Insulin (Insulin, INS) enzyme linked immunoassay kit, lot number: 20220122, nanjing, built Biotech, inc.

Electronic analytical balance (LE 204E/02), mettler Toledo, USA; sinocare Sanona quasi-glucometer, sanno biosensing, inc.; rotary evaporator (RE-200A), shanghai Yangrong Biochemical apparatus factory; full automatic enzyme calibration apparatus (ELX-800), BIO-TEK, USA.

Clean-grade male Kunming mice, weighing (35 + -3) g, provided by Schlekschada laboratory animals Co., ltd, animal production license number: SCXK (Xiang) 2019-0004. All experimental animals are raised in cages, placed in a constant temperature and humidity chamber at the temperature of 24 +/-2 ℃, and are alternately lighted/darkened for 12 hours, so that drinking water is freely taken.

2. Experimental methods

Grouping, modeling and dosing:

60 mice were selected and randomized after 7d acclimation into Normal Control (NC) and model groups (n = 50). The mice in the NC group are fed with common feed, the mice in the model group are fed with high-fat high-sugar feed, after the mice in the model group are fed with diet-induced insulin resistance for 8 weeks, the mice in the model group are injected with STZ (75 mg/kg) in the abdominal cavity, and the mice with hyperglycemia after successful modeling are divided into a diabetes model group (MC), a positive control group (PC), a low-dose group (L) of a traditional Chinese medicine composition, a medium-dose group (M) of the traditional Chinese medicine composition and a high-dose group (H) of the traditional Chinese medicine composition; wherein, the L group, the M group and the H group are respectively irrigated with low, medium and high doses of the traditional Chinese medicine composition prepared in the example 1, 200mg/kg of metformin is administered to the PC group, and physiological saline with the same volume is administered to the MC group and the NC group for continuous irrigation for 4 weeks.

And (4) observation indexes are as follows:

mental status, body weight, blood glucose, serum insulin levels, oral glucose tolerance, total cholesterol, triglycerides, low density lipoprotein cholesterol and high density lipoprotein cholesterol were measured in 6 groups of mice, respectively.

The experimental indexes are as follows:

(1) Before molding, all groups of mice have good mental state, white and bright hair, and normal drinking water, food intake and defecation. After the model building is successful, except for the normal group, the mice in each group show different degrees of lassitude, withered hair color, polydipsia, diuresis, polyphagia, reduction of activity and the like, and the symptoms of 'three high and one few' appear. After the results of the dose groups of the traditional Chinese medicine composition prepared by the metformin and the example 1 are dry, the symptoms of mice in each group are relieved, the activity is enhanced, the spirit is improved, and the hair color glossiness is improved compared with that of an MC group, which shows that the traditional Chinese medicine composition prepared by the invention is beneficial to maintaining the blood sugar health level.

(2) The effect on mouse body mass is shown in FIG. 1.

As can be seen from fig. 1, the MC group showed a downward trend in body weight (P < 0.01) compared to the normal group (NC), which is probably due to abnormal metabolism of structural proteins of diabetic mice, resulting in a decrease in food intake; the weight of the PC group gradually increased compared with that of the MC group; after 1 month of intervention, the traditional Chinese medicine composition prepared in example 1 has different weight reducing effects in each administration group compared with the MC group, especially in the H group (P < 0.01).

(3) The results of the effect on fasting plasma glucose concentration in mice are shown in FIG. 2.

As can be seen from FIG. 2, the FBG of the NC group mice is substantially unchanged and is between 5 and 7 mmol/L. Compared with the NC group, the MC group had a significant increase in FBG (P < 0.01), and was maintained in a hyperglycemic state, i.e., the model was considered successful and the stability of the diabetes model was good. After the intervention of each dosage of the traditional Chinese medicine composition prepared in the embodiment 1 for 1 month, compared with the MC group, the FBGs of the mice in the PC group and the L, M and H groups of the recipe are all reduced, and the difference is of great statistical significance (P < 0.01). The dosage groups of the traditional Chinese medicine composition prepared by the invention can better regulate and control the fasting blood glucose concentration level of T2DM mice, and present a certain dose-effect relationship, and the ability of maintaining the healthy blood glucose level is reduced along with the reduction of the dosage.

(4) The effect on mouse oral glucose tolerance and area under the curve results are shown in figure 3.

As can be seen from fig. 3, within 120min of the test, the blood glucose concentration of each group of mice tended to increase first and then decrease, and the blood glucose of the MC group was significantly increased (P < 0.01) compared to the NC group, indicating that T2DM mice had impaired glucose tolerance. Blood glucose values of each group began to fall back to the vicinity of the initial value at 120min, but blood glucose levels of the MC group were high, and blood glucose of mice of each administration group tended to fall back to the initial value, wherein blood glucose of mice of the H group was significantly reduced.

(5) The effect on blood lipid metabolism in mice is shown in table 1.

TABLE 1 Effect on blood lipid metabolism in diabetic mice

As can be seen from Table 1, the MC group showed significantly higher TC, TG and LDL-C contents (P < 0.01) than the NC group, but the HDL-C was significantly lower than the control group, indicating that the lipid metabolism of the mice under hyperglycemia was abnormal. After the administration, the serum TC, TG and LDL-C of the PC group and the mice of the L, M and H groups are reduced to different degrees (P < 0.05) compared with the MC group, wherein the concentration reduction of the TC and the LDL-C of the L group is not obvious (P > 0.05). The HDL-C content of each group is obviously increased compared with that of the MC group, and the function of accelerating the metabolism of TC is achieved. Therefore, each dose group can regulate the blood lipid level of the mouse to a certain extent, the low dose group has weaker capability, the M group and the PC group have similar blood lipid regulation capability, and the H group has more obvious effect on improving the blood lipid level.

(6) The effects on mouse insulin, HOMA-IR are shown in FIGS. 4-5.

As can be seen from FIGS. 4 to 5, the serum insulin levels were decreased and the insulin resistance was significantly increased in each group as compared with the NC group. Wherein, the serum insulin level of the MC group is remarkably reduced compared with that of the NC group (P < 0.01), but the insulin resistance is remarkably enhanced (P < 0.01); the difference in serum insulin levels between the dose-administered groups was not statistically significant (P > 0.05) compared to the MC groups, but insulin resistance was significantly lower than the MC groups. The effect of the traditional Chinese medicine composition for maintaining the healthy blood sugar level is not realized by promoting insulin secretion, but is realized by reducing the insulin sensitivity index and improving the insulin resistance level of a T2DM mouse so as to achieve the purpose of maintaining the healthy blood sugar level.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims

1. A traditional Chinese medicine composition helpful for maintaining blood sugar health level is characterized by comprising the following raw materials in parts by weight: 10-20 parts of codonopsis pilosula, 15-25 parts of rhizoma polygonati, 15-25 parts of radix puerariae, 15-25 parts of Chinese yam and 6-15 parts of pseudo-ginseng.

2. The traditional Chinese medicine composition helpful for maintaining healthy blood sugar level according to claim 1, comprising the following raw materials in parts by weight: 15 parts of codonopsis pilosula, 20 parts of rhizoma polygonati, 20 parts of radix puerariae, 20 parts of Chinese yam and 10 parts of pseudo-ginseng.

3. The method of preparing a herbal composition of claim 1 or 2, for assisting in maintaining healthy blood glucose levels, comprising the steps of:

(4) Mixing the primary filtrate obtained in the step (2) and the secondary filtrate obtained in the step (3), filtering, standing for 8-12h, concentrating the obtained supernatant under reduced pressure, and drying to obtain the traditional Chinese medicine composition which is helpful for maintaining the healthy level of blood sugar.

4. The method for preparing a herbal composition for aiding in the maintenance of healthy levels of blood glucose according to claim 3, wherein step (1) is performed with a 20-50 mesh screen.

5. The method for preparing a herbal composition for assisting in maintaining healthy blood glucose levels as claimed in claim 3, wherein in the step (2), the volume ratio of herbal powder to purified water is 1:7-10.

6. The method for preparing a Chinese medicinal composition for helping to maintain healthy level of blood glucose according to claim 3, wherein in the step (3), the volume ratio of the filter residue to the purified water is 1:3-5.

7. The method of claim 3, wherein in step (4), the concentration is performed at 50-80 ℃ under reduced pressure until the relative density is 1.1-1.3.

8. The method for preparing a Chinese medicinal composition for helping to maintain healthy level of blood glucose as claimed in claim 3, wherein in the step (4), vacuum drying or spray drying is adopted.

9. Use of the Chinese medicinal composition for helping to maintain healthy blood glucose level according to claim 1 or 2 for preparing health food for helping to maintain healthy blood glucose level.

10. A health food for helping to maintain healthy level of blood glucose, comprising the Chinese medicinal composition for helping to maintain healthy level of blood glucose of claim 1 or 2 and cereal flour.