CN101336974B - Traditional Chinese medicine for reducing blood sugar and regulating lipid with overall regulation of body metabolism and preparation method thereof - Google Patents
Traditional Chinese medicine for reducing blood sugar and regulating lipid with overall regulation of body metabolism and preparation method thereof Download PDFInfo
- Publication number
- CN101336974B CN101336974B CN 200810048729 CN200810048729A CN101336974B CN 101336974 B CN101336974 B CN 101336974B CN 200810048729 CN200810048729 CN 200810048729 CN 200810048729 A CN200810048729 A CN 200810048729A CN 101336974 B CN101336974 B CN 101336974B
- Authority
- CN
- China
- Prior art keywords
- chinese medicine
- rhizoma coptidis
- parts
- cortex cinnamomi
- regulating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a traditional Chinese medicine which has the effects of reducing blood sugar and regulating lipid and the metabolic function of regulating the overall organism, and the preparation method thereof. The traditional Chinese medicine is a preparation prepared from membranous milk vetch root, gold theragran, kudzu root, mulberry leaf, common fenugreek seed, rhizoma coptidis and cinnamon by the proportion. The preparation method comprises the following steps: (1) extracting rhizoma coptidis and kudzu root with ethanol separately, mixing the extractives, decompressing, recovering ethanol completely and obtaining the mixed extractive of the active components of rhizoma coptidis and kudzu root; (2) pulverizing cinnamon into coarse powder, extracting by adopting the distillation method and obtaining the cinnamon volatile oil and the cinnamon residue liquid; and (3) adding common fenugreek seed, membranous milk vetch root, gold theragran and mulberry leaf to the cinnamon residue liquid, decocting with the distilled water separately, mixing to decoct, decompressing, concentrating, further adding the mixed extractive of the active components of rhizoma coptidis and kudzu root and the cinnamon volatile oil, vacuum drying, and finally preparing into powder, granules, tablets or capsules by adopting the conventional method. The traditional Chinese medicine can fundamentally regulate the metabolic disturbance of the organism, and meanwhile, the traditional Chinese medicine has significant effects of reducing blood sugar, regulating lipid and preventing complicating diseases, and has the advantages of precise effect, little toxic or side effect, safety and reliability.
Description
Technical field
The present invention relates to the Chinese herbal medicine is the kidney invigorating, detoxifcation, the hypoglycemic medicine that feedstock production forms, and is meant a kind of hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism and preparation method thereof especially.
Background technology
Diabetes are commonly encountered diseases, frequently-occurring diseases of 21 century, the change of Along with people's life style, and the minimizing of physical exertion, the increase of aged tendency of population, the increasing of hypertension chronic diseases, the aggravation of overweight and fat phenomenon, its sickness rate is in continuous rising.According to statistics, the total number of persons of global diabetics in 2000 is about 1.71 hundred million, surpasses 2.3 hundred million at present, and just increases 7,000,000 patients' speed increase newly with every year.Expect the year two thousand thirty, diabetics will increase to 3.66 hundred million.The prevalence of China's diabetes was about 1.0%, 1989 year in 1976 and is about 2.0%, 1996 year and is about 3.2%, almost annually rose 0.1%, estimated that prevalence in 2010 will reach 4%.
The harm of diabetes is mainly at its chronic complicating diseases of following, and the heart, brain, kidney late complication that long-term hyperglycemia causes are very harmful, have become human " the third-largest killer ".Like diabetic nephropathy, diabetic retinopathy, neuropathy, diabetic foot and diabetic cardiac damage etc., these complication are to cause a large amount of patient's deformity and main causes of death, and mortality rate is only second to cardiovascular disease and tumor.For the type 2 diabetes mellitus patient, the life expectancy value possibly reduce 5～10 years old, and wherein the women shows more obviously.Therefore, searching can the overall regulation of body metabolism disorder, can prevent and treat again that diabetic complication takes place and the hypoglycemic fat-regulating pharmaceutical composition of development is the target that vast medical research worker is pursuing always.But the medicine of domestic and international existing treatment diabetes exists following problem at present:
From the angle of doctor trained in Western medicine, the pharmaceutical progress of treatment type 2 diabetes mellitus is quick, and it is of a great variety go on the market, to what defect of insulin secretion and insulin resistant were fallen ill link sulphanylureas and biguanides is arranged; Delay the alpha-glucosidase inhibitor that has of intestinal glucose absorption; That improves insulin sensitivity has a thiazolidinediones medicine.But all to be some links to onset diabetes mechanism work for they, only cures the symptoms, not the disease, and the glycolipid metabolism that can not improve diabetics on the whole is disorderly.
And these Western medicine more or less all exist different toxic and side effects.Like in January, 1971, first glitazone medicine troglitazone (genus thiazolidinediones) comes out, and regrettably after listing just 3 years, just withdraws from market from the whole world rapidly because of serious liver toxicity even liver dead 71 examples that decline.The lower euglycemic agent of toxicity has then appearred in succession; Represent medicine that rosiglitazone, pioglitazone etc. are arranged; Be characterized in that blood sugar reducing function is strong; Other guide such as hypertension, disorders of lipid metabolism etc. to metabolism syndrome can produce favorable influence simultaneously, and using this type medicine is " the many birds of a stone, lifelong benefit ".Yet As time goes on, also there is hepatotoxic case the untoward reaction had palpitation of its existence, headache, pruritus, diarrhoea, edema, dyspnea, weight increase or the like to occur frequently.The side effect such as gastrointestinal reaction of sulphanylureas and biguanides also are difficult problems that perplexs diabetics for a long time.
Simultaneously; These Western medicine to the chronic complicating diseases of diabetics after one's own heart, brain, kidney complication then have basically no preventive and therapeutic effect; Promptly can not fundamentally solve the harm problem of type 2 diabetes mellitus and chronic complicating diseases thereof, not good enough to the therapeutic effect of other metabolism disorder symptom of type 2 diabetes mellitus yet.
From the angle of the traditional Chinese medical science, also explored the Chinese patent medicine of a large amount of treatment type 2 diabetes mellitus up to now, sophisticated kind such as diabetes pill, JIANGTANGSHU, YUQUAN WAN, blood sugar lowering first sheet, manna diabetes capsule, SHENQI JIANGTANG sheet and KELENING capsule etc.But these Chinese patent medicines are relatively poor to the pathogenetic specific aim of different diabeticss on the one hand; The power of supplementing QI and nourishing YIN is not enough; Therapeutic effect is not enough or not good enough; These Chinese patent medicines or contain western medicine composition or have Liver and kidney toxicity or have gastrointestinal side effect on the other hand, to a great extent limit the expansion of its application.
Summary of the invention
The object of the invention is exactly will provide that a kind of therapeutic effect is definite, toxic and side effects is little, take the safe and reliable hypoglycemic fat-regulating Chinese medicine of overall regulation of body metabolism and preparation method thereof that has.
For realizing above-mentioned purpose; Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism proposed by the invention is the medicament of being processed effective ingredient by following materials of weight proportions: 1.875～7.5 parts of the Radixs Astragali, 2.25～9.0 parts of Herb Gynostemmae Pentaphylli, 1.875～7.5 parts of Radix Puerariaes, 1.5～6.0 parts on Folium Mori, 1.5～6.0 parts of Semen Trigonellaes, 0.75～3.0 part of Rhizoma Coptidis, 0.375～1.5 part of Cortex Cinnamomi.
The above-mentioned preferred weight proportioning of processing the raw material of medicament of the present invention is: 1.875～3.75 parts of the Radixs Astragali, 2.25～4.5 parts of Herb Gynostemmae Pentaphylli, 1.875～3.75 parts of Radix Puerariaes, 1.5～3.0 parts on Folium Mori, 1.5～3.0 parts of Semen Trigonellaes, 0.75～1.5 part of Rhizoma Coptidis, 0.375～0.75 part of Cortex Cinnamomi.
The above-mentioned optimum weight proportioning of processing the raw material of medicament of the present invention is: 1.875 parts of the Radixs Astragali, 2.25 parts of Herb Gynostemmae Pentaphylli, 1.875 parts of Radix Puerariaes, 1.5 parts on Folium Mori, 1.5 parts of Semen Trigonellaes, 0.75 part of Rhizoma Coptidis, 0.375 part of Cortex Cinnamomi.
In the above-mentioned raw material of processing medicament of the present invention, Rhizoma Coptidis is preferably adopted all preferable Rhizoma Coptidis of quality and drug effect.
Medicament of the present invention can be any acceptable forms in the pharmaceutics, and the dosage form of selecting for use usually is a kind of in powder, granule, tablet or the capsule.
Hypoglycemic fat-regulating preparation method of Chinese medicine with overall regulation of body metabolism according to the invention comprises the steps:
1) get Rhizoma Coptidis and Radix Puerariae by above-mentioned weight proportion, mix homogeneously adopts the ethanol gradation to extract, merge extractive liquid,, and decompression recycling ethanol obtains Rhizoma Coptidis kudzu vine root mixed extract to there not being the alcohol flavor;
2) get Cortex Cinnamomi by above-mentioned weight proportion, be broken into coarse powder, adopt steam distillation to extract, obtain Cortex Cinnamomi volatile oil and Cortex Cinnamomi residue water liquid;
3) get Semen Trigonellae, the Radix Astragali, Herb Gynostemmae Pentaphylli and Folium Mori by above-mentioned weight proportion; Add step 2) gained Cortex Cinnamomi residue water liquid, adopt the distilled water gradation to decoct collecting decoction; Concentrating under reduced pressure; Add step 1) gained Rhizoma Coptidis kudzu vine root mixed extract and step 2 again) the gained Cortex Cinnamomi volatile oil, vacuum drying is made into powder, granule, tablet or capsule by conventional method at last.
Chinese medicine of the present invention is based on the understanding of motherland's medical science to diabetes mechanism, and with reference to the modern pharmacological research achievement, the utilization differential diagnosis in tcm is theoretical; Sincerely keep old maxim, widely collect crowd side, control from the poison opinion; And adhere to the detoxifcation the kidney invigorating is run through treatment principle all the time, from motherland medicine treasure-house, filter out have detoxifcation, the medicine material combination of the kidney invigorating, blood sugar lowering; Putting into practice by theory of Chinese medical science, select the essence to the greatest extent, is starting point with the risk factor of eliminating diabetic complication; Blood sugar lowering, detoxifcation, the kidney invigorating synergism; Give full play to its regulating action, control hyperglycemia, hypertension, hyperlipidemia, high blood viscosity and hyperuricemia comprehensively, and then reach and slow down diabetic complication, eliminate the purpose of patient's three-many-one-little clinical symptoms rapidly organism metabolism.Its advantage is embodied in following three aspects:
First blood sugar lowering: two basic links of type 2 diabetes mellitus morbidity are defect of insulin secretion and insulin resistant, and the present invention is through extracting the active active ingredient in the raw material, like puerarin, the many glycosides of the Radix Astragali, berberine etc.; Make these active ingredients get into tissue and cell with the bioactive molecule state; Mutual synergism: adjust the normal secretion of insulin on the one hand, promote the biological effect that insulin is brought into normal play on the other hand, improve insulin resistant; Strengthen the sensitivity of Insulin receptor INSR; Promote itself and the bonded affinity of Insulin receptor INSR, thereby help utilization and the absorption of peripheral tissues, realize steady blood sugar lowering glucose.
It two is detoxifcations: modern study shows, it is bright that " poison " core mechanism in diabetes is that " pyretic toxicity " disappears, the lung Tianjin of burning on the pyretic toxicity, and middle misfortune gastric juice, following consumption kidney water then three types of diabetes all show; In addition; Glycosylation due to the high sugar causes that the raise tumor necrosis factor that causes excessive secretion in what is called " fat toxicity " and the body of what is called " sugared toxicity ", free fatty (FFA) brings out what is called " fire-toxin property " with radical damage and also in the incidence and development of type 2 diabetes mellitus, plays an important role, and this just treats type 2 diabetes mellitus theoretical foundation is provided for improve insulin resistant with " heat-clearing and toxic substances removing " Chinese medicine.The Radix Astragali among the present invention is very important Qi-tonifying drug, the insulin resistant that its effective ingredient astragalus polysaccharides ability ameliorate tumor necrosis factor-alpha produces animal; Rhizoma Coptidis among the present invention then has the effect of good heat-clearing and toxic substances removing, antioxidation and removing free radical; Herb Gynostemmae Pentaphylli, Cortex Cinnamomi, Rhizoma Coptidis have the effect that reduces free fatty in addition.
It three is the kidney invigorating: kidney master water improving inspiration by invigorating kidney-QI, main bone is given birth to marrow, presides over the metabolism of total body water liquid, water liquid metabolic balance in the adjustable body, kidney is the root of the five internal organs, to the whole body internal organs play a part to nourish, warm.It is the source of causing a disease that diabetics is suffered from a deficiency of the kidney, and the Semen Trigonellae active ingredient among the present invention not only has the mechanism of blood sugar lowering, can also temperature male wind-supplying kidney, and then the whole body function is vigorous by force for kidney, can directly eliminate the disease of suffering from a deficiency of the kidney of diabetes.
The present invention is a starting point with the risk factor of eliminating diabetic complication; The effect of comprehensive above blood sugar lowering, detoxifcation and the kidney invigorating three aspects can be found: the curative effect of an aspect has huge impetus to other curative effect of two aspects; Detoxifcation is beneficial to blood sugar lowering and the kidney invigorating, and the kidney invigorating then is beneficial to detoxifcation and blood sugar lowering, and blood sugar lowering also is of value to detoxifcation and the kidney invigorating; The effect of blood sugar lowering, detoxifcation and the kidney invigorating is advanced side by side, and three aspect integral body link, correlate each other.Both helped warm drugs ascending kidney water less, strengthened enriching yin and nourishing kidney to the diabetes and the relevant pathogenic characteristic of suffering from a deficiency of the kidney; Cause what is called " sugared toxicity " to the glycosylation due to the high sugar again and use heat and toxic materials clearing away medicine to remove interior free yl; Also raise to free fatty (FFA) that the tumor necrosis factor cause what is called " fat toxicity " and the interior excessive secretion of body and radical damage bring out what is called " fire-toxin property " and medicine that compatibility relieves inflammation or internal heat.Thereby alleviate oxidative stress to the damage of beta Cell of islet and target organ, the reparation of promotion beta Cell of islet; Promote the utilization of target tissue simultaneously to insulin; Impel the variation of organismic internal environment generation matter, and then realize one's own department or unit treatment diabetes, fundamentally slow down and eliminate complication and improve the effect of clinical symptoms.And former material assembly of the present invention all comes from natural plants, and checking already has basically no toxic and side effects, takes safe and reliable for a long time.
Description of drawings
Fig. 1 is the islets of langerhans form HE dyeing microstructure sketch map of each group rat, wherein: the microstructure of the corresponding normal rats islets of langerhans of Fig. 1 a, the microstructure of the corresponding model group rat Langerhans islet of Fig. 1 b, the corresponding low dose group rat Langerhans islet of the present invention microstructure of Fig. 1 c.
Fig. 2 respectively organizes rat Langerhans islet Insulin and PDX-1 protein expression microstructure sketch map for what immunofluorescence showed, and wherein: Fig. 2 a, Fig. 2 b and Fig. 2 c distinguish Insulin, PDX-1 and the Insulin+PDX-1 protein expression of corresponding normal rats; Fig. 2 d, Fig. 2 e and Fig. 2 f be Insulin, PDX-1 and the Insulin+PDX-1 protein expression of corresponding model group rat respectively; Fig. 2 g, Fig. 2 h and Fig. 2 i be Insulin, PDX-1 and the Insulin+PDX-1 protein expression of corresponding low dose group rat of the present invention respectively.
Fig. 3 carries out the view that observation post takes for the eyes of each group rat before platycoria; Wherein: the corresponding normal rats of Fig. 3 a; The corresponding model group rat of Fig. 3 b, the corresponding high dose group rat of the present invention of Fig. 3 c, dose groups rat among the corresponding the present invention of Fig. 3 d; The corresponding low dose group rat of the present invention of Fig. 3 e, the corresponding YIQITANGKANG group of Fig. 3 f rat.
Fig. 4 carries out view that observation post take with operating microscope for the eyes of each group rat behind platycoria; Wherein: the corresponding normal rats of Fig. 4 a; The corresponding model group rat of Fig. 4 b, the corresponding high dose group rat of the present invention of Fig. 4 c, dose groups rat among the corresponding the present invention of Fig. 4 d; The corresponding low dose group rat of the present invention of Fig. 4 e, the corresponding YIQITANGKANG group of Fig. 4 f rat.
The specific embodiment
Hypoglycemic fat-regulating Chinese medicine that the present invention is had the overall regulation of body metabolism below in conjunction with accompanying drawing and embodiment and preparation method thereof is done further to describe in detail:
Embodiment 1:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 1.875kg, Herb Gynostemmae Pentaphylli 2.25kg, Radix Puerariae 1.875kg, Folium Mori 1.5kg, Semen Trigonellae 1.5kg, Rhizoma Coptidis 0.75kg, Cortex Cinnamomi 0.375kg.
Embodiment 2:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 2.25kg, Herb Gynostemmae Pentaphylli 3.55kg, Radix Puerariae 2.15kg, Folium Mori 2.25kg, Semen Trigonellae 2.25kg, Rhizoma Coptidis 1.00kg, Cortex Cinnamomi 0.45kg.
Embodiment 3:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 3.25kg, Herb Gynostemmae Pentaphylli 2.25kg, Radix Puerariae 3.25kg, Folium Mori 1.80kg, Semen Trigonellae 1.80kg, Rhizoma Coptidis 1.25kg, Cortex Cinnamomi 0.55kg.
Embodiment 4:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 3.75kg, Herb Gynostemmae Pentaphylli 4.50kg, Radix Puerariae 2.55kg, Folium Mori 2.80kg, Semen Trigonellae 2.50kg, Rhizoma Coptidis 1.50kg, Cortex Cinnamomi 0.75kg.
Embodiment 5:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 4.25kg, Herb Gynostemmae Pentaphylli 6.50kg, Radix Puerariae 4.55kg, Folium Mori 4.80kg, Semen Trigonellae 5.50kg, Rhizoma Coptidis 2.50kg, Cortex Cinnamomi 1.25kg.
Embodiment 6:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 5.45kg, Herb Gynostemmae Pentaphylli 5.50kg, Radix Puerariae 6.50kg, Folium Mori 3.75kg, Semen Trigonellae 3.50kg, Rhizoma Coptidis 2.25kg, Cortex Cinnamomi 1.00kg.
Embodiment 7:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 6.50kg, Herb Gynostemmae Pentaphylli 8.50kg, Radix Puerariae 7.50kg, Folium Mori 5.75kg, Semen Trigonellae 4.50kg, Rhizoma Coptidis 1.75kg, Cortex Cinnamomi 0.85kg.
Embodiment 8:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 7.50kg, Herb Gynostemmae Pentaphylli 3.50kg, Radix Puerariae 5.50kg, Folium Mori 3.75kg, Semen Trigonellae 2.50kg, Rhizoma Coptidis 2.00kg, Cortex Cinnamomi 1.25kg.
Embodiment 9:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 2.50kg, Herb Gynostemmae Pentaphylli 3.50kg, Radix Puerariae 3.50kg, Folium Mori 1.75kg, Semen Trigonellae 2.50kg, Rhizoma Coptidis 2.25kg, Cortex Cinnamomi 0.75kg.
Embodiment 10:
Hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is the medicament that is prepared from Radix Astragali 1.875kg, Herb Gynostemmae Pentaphylli 3.25kg, Radix Puerariae 2.25kg, Folium Mori 1.75kg, Semen Trigonellae 2.25kg, Rhizoma Coptidis 1.25kg, Cortex Cinnamomi 1.25kg.
The hypoglycemic fat-regulating preparation method of Chinese medicine that has the overall regulation of body metabolism among above-mentioned each embodiment comprises the steps:
1) get Rhizoma Coptidis and two kinds of raw materials of Radix Puerariae by above-mentioned weight proportion, broken mix homogeneously, adopting concentration of volume percent is that 70～75% ethanol divides three extraction: the extraction time first time is 2.5～3.0 hours; Extraction time is 1.5～2.0 hours for the second time; Extraction time is 1.0～1.5 hours for the third time, to extract its effective ingredient to greatest extent, merges each time extracting solution; Reclaim under reduced pressure ethanol wherein obtains Rhizoma Coptidis kudzu vine root mixed extract to there not being the alcohol flavor.
2) get the Cortex Cinnamomi raw material by above-mentioned weight proportion, be ground into coarse powder, adopt steam distillation to extract the effective ingredient in the Cortex Cinnamomi, obtain Cortex Cinnamomi volatile oil and Cortex Cinnamomi residue water liquid.
3) get Semen Trigonellae, the Radix Astragali, Herb Gynostemmae Pentaphylli and four kinds of raw materials of Folium Mori by above-mentioned weight proportion, add step 2) gained Cortex Cinnamomi residue water liquid, adopt the distilled water decocting method to divide three extractions: decocting time is 2.0～2.5 hours for the first time; Decocting time is 1.0～1.5 hours for the second time; Decocting time is 1.0～1.5 hours for the third time, fully to obtain effective ingredient wherein, merges each time decocting liquid; Concentrating under reduced pressure; Add step 1) gained Rhizoma Coptidis kudzu vine root mixed extract and step 2 again) the gained Cortex Cinnamomi volatile oil, vacuum concentration is dry, is made into powder, granule, tablet or capsule by conventional method at last.
By the hypoglycemic fat-regulating Chinese medicine that above-mentioned method for preparing obtained, diabetics can be taken according to the dose size of doctor's advice.Generally speaking, be example with the capsule of every 0.4g, adult every day three times, each two to three, warm boiled water, two months is a course of treatment.
In order to confirm the present invention, further introduce the result that hypoglycemic fat-regulating Chinese medicine of the present invention is obtained below in the research of each item pharmacological basis in the excellent curative effect aspect the overall regulation of body metabolism:
One, Chinese medicine of the present invention is to the influence of type 2 diabetes mellitus rat carbohydrate tolerance, empty stomach and post-prandial glycemia
Laboratory animal is selected the male Wistar rat at about 2 monthly ages, and body weight 220～250g purchases the Animal Experimental Study center in Hubei Province.Elder generation's adaptability fed for 2 weeks, 70 rat ad libs, and with the dosage of 30mg/kg through tail vein injection STZ.After normal diet fed for 2 weeks, filter out the rat of 54 impaired glucose tolerance, be divided into following 5 groups at random: 11 of (1) low dose group of the present invention, irritate clothes the present invention with 1.025g/kgd; (2) 11 of dose groups among the present invention are irritated clothes the present invention with 2.05g/kgd; (3) high dose group of the present invention is 11, irritates clothes the present invention with 4.1g/kgd; (4) the YIQITANGKANG group is 10, irritates the clothes YIQITANGKANG with 2.67g/kgd; (5) diabetic model group is 11, irritates the PBS solution that the same volume of clothes contains 0.5% carboxymethyl cellulose.More than 5 groups of rats all feed with high fat diet (sucrose: Adeps Sus domestica: milk powder: egg: normal feedstuff=30: 40: 8: 4: 63).(6) other 10 rats do not give any intervention, as normal group, feed with normal diet.
After the pharmaceutical intervention 100 days; Observe and respectively organize the influence of medicine indexs such as type 2 diabetes mellitus rat glycolipid metabolism, insulin sensitivity and anti-oxidation stress states; 4 rats deaths are arranged in the experimentation; Each time point blood sugar level adopts glucose oxidase method to detect, and each result who organizes rat oral glucose tolerance test mensuration sees table 1.
Respectively organize the tolerance test of rat oral glucose and measure comparative parameter and can know from table 1 is listed: Chinese medicine of the present invention can effectively improve the type 2 diabetes mellitus rat impaired glucose tolerance, reduce on an empty stomach and post-prandial glycemia, can be used for treating diabetes; Particularly the strongest with the effect of low dose group of the present invention, be superior to Chinese medicine YIQITANGKANG group.
Table 1: each is organized the tolerance test of rat oral glucose and measures relatively (χ ± s) of result
*P＜0.01, the vs normal group;
△P＜0.05,
△ △P＜0.01, the vs model group;
#P＜0.05, vs YIQITANGKANG group
Two, Chinese medicine of the present invention is to the influence of type 2 diabetes mellitus rat fat
Animal experiment method is the same.TC, TG adopt Enzymology method to detect; HDL-C, LDL-C adopt the magnesium P wolframic acid sedimentation method to detect; Apo A I, Apo B adopt immune turbidimetry to detect; FFA adopts the DDTC development process to detect; Each result who organizes rat blood serum lipid parameter mensuration sees table 2.
Measure comparison as a result and can know from the listed rat blood serum lipid parameter of respectively organizing of table 2: Chinese medicine of the present invention can effectively improve the blood fat disorder of type 2 diabetes mellitus rat, and is the strongest with the effect of low dose group, is superior to Chinese medicine YIQITANGKANG group.
Table 2: each is organized the rat blood serum lipid parameter and measures relatively (χ ± s) of result
*P＜0 01, the vs normal group;
△P＜005,
△ △P＜0.01, the vs model group;
#P＜0.05,
##P＜0.01, vs YIQITANGKANG group
Three, Chinese medicine of the present invention is to the effect of type 2 diabetes mellitus rat insulin, islet cells
1. animal experiment method is the same.Serum insulin levels adopts radioimmunology to detect, and each result who organizes rat insulin release test mensuration sees table 3.Respectively organize the comparison of rat insulin release test location parameter and can know from table 3 is listed: Chinese medicine of the present invention can improve hyperinsulinemia; Recover to secrete mutually in first o'clock; The function of protection type 2 diabetes mellitus rat Langerhans islet β cell, the strongest with the low dose group effect especially, be superior to Chinese medicine YIQITANGKANG group.
Table 3: each is organized the rat insulin release test and measures relatively (χ ± s) of result
*P＜0.01, the vs normal group;
△P＜0.05,
△ △P＜0.01, the vs model group;
#P＜0.05,
##P＜0.01, vs YIQITANGKANG group
2. animal experiment method is the same.Each time point blood sugar level adopts glucose oxidase method to detect, and each result who organizes rat insulin tolerance test mensuration sees table 4.Respectively organize the comparison of rat insulin tolerance test location parameter and can know from table 4 is listed: Chinese medicine of the present invention can effectively improve the insulin sensitivity of type 2 diabetes mellitus rat, and is especially the strongest with the low dose group effect, is superior to Chinese medicine YIQITANGKANG group.
Table 4: each is organized the rat insulin tolerance test and measures relatively (χ ± s) of result
*P＜0.01, the vs normal group;
△P＜0.05,
△ △P＜0.01, the vs model group;
#P＜0.05, vs YIQITANGKANG group
3. animal experiment method is the same.Adopt the HE staining to detect, each is organized the islets of langerhans form HE dyeing microstructure of rat and sees Fig. 1.Visible from the islets of langerhans microscopic morphology of respectively organizing rat shown in Figure 1: normal rats islets of langerhans clear border is neat, the complete form rule, and island shape is plentiful; The island cell distribution is even, big or small homogeneous, each nucleus ovalize; Endochylema is abundant, and islets of langerhans quantity and island inner cell number average are more; The model group rat Langerhans islet differs in size, obviously atrophy, and it is mellow and full that profile is owed, and it is clear and irregular that owe on the border, and the island cell cytosol reduces, and in the part islet cells endochylema vacuolar degeneration is arranged, karyopyknosis, islets of langerhans quantity significantly descends in the visual field, volume-diminished; Low dose group pancreas in rat of the present invention is organized comparatively intact, and is similar with the pancreatic tissue of normal group, and islet tissue is more complete in the visual field, and boundary is more clear, and cell volume is more normal, and blood capillary is abundanter in the island, and island shape is fuller.Explain that Chinese medicine of the present invention has protective effect to the type 2 diabetes mellitus islets of langerhans.
4. animal experiment method is the same.Adopt the immune fluorescence grouping analytical method, each is organized the Insulin and the microstructure of PDX-1 protein expression of rat Langerhans islet tissue and sees Fig. 2.Can know from coloration result shown in Figure 2: be strong positive the islet cells of normal rats, the strong (green-Insulin albumen of fluorescence; Redness-PDX-1 albumen, down together), be the weak positive in the islet cells of model group rat, fluorescence weak (red+green), visible fluorescence obviously strengthens after the administration, and islets of langerhans Insulin of hints model group rat and PDX-1 protein expression reduce; And Insulin and the PDX-1 protein expression of low dose group rat of the present invention after treatment significantly increases; Explain that Chinese medicine of the present invention can increase the quantity of type 2 diabetes mellitus Islet cells; Increase insulin secretion, its mechanism of action maybe be relevant with the proteic expression of increase islets of langerhans PDX-1.
Four, Chinese medicine of the present invention is to the influence of type 2 diabetes mellitus rat oxidative stress status
1. animal experiment method is the same.HbA1C detects with HPLC; SOD, GSH-px activity measure with chemical colorimetry; NO, MDA are with colorimetric method for determining; Each is organized rat whole blood HbA1C content and serum NO and oxidative stress index of correlation mensuration result and sees table 5.Measuring comparison as a result from the listed index of correlation of respectively organizing rat of table 5 can know: Chinese medicine of the present invention can improve type 2 diabetes mellitus rat oxidative stress status; Alleviate radical pair tissue and cells injury; Especially the strongest with the low dose group effect, be superior to Chinese medicine YIQITANGKANG group.
Table 5: each is organized rat whole blood HbA1C content and serum NO and oxidative stress index of correlation and measures relatively (χ ± s) of result
*P＜0.01, the vs normal group;
△P＜0.05,
△ △P＜0.01, the vs model group;
#P＜0.05,
##P＜0.01, vs YIQITANGKANG group
2. animal experiment method is the same.HbA1C detects with HPLC; SOD, GSH-px activity measure with chemical colorimetry; NO, MDA are with colorimetric method for determining; Each organizes pancreas in rat tissue SOD, GSH-px is active, and MDA and NO assay result see table 6.Can know from the listed index of correlation comparison of respectively organizing the pancreas in rat tissue of table 6: MDA, NO obviously raise than normal group in the model group pancreas in rat tissue homogenate, and SOD, GSH-px significantly reduce (P＜0.05); With model group relatively, MDA in the basic, normal, high dose groups pancreas in rat of the present invention tissue homogenate, NO are lower, and SOD, GSH-px content are higher, explain that Chinese medicine of the present invention can strengthen the islet tissue oxidation resistance, thereby the protection islets of langerhans are injury-free.
Table 6: each organizes pancreas in rat tissue SOD, GSH-px is active, and the comparison of MDA and NO content (x ± s)
*P＜0.01, the vs normal group; △ P＜0.05, △ △ P＜0.01, vs model group; #P＜0.05, ##P＜0.01, vs YIQITANGKANG group
Five, Chinese medicine of the present invention is to the influence of early stage type 2 diabetes mellitus rat kidney
Animal experiment method is the same.BUN measures with urease method; Urinaryalbumin, β 2-MG adopt radioimmunology to detect; Creatinine adopts picric acid method to detect; Each is organized the testing result such as kidney/body weight, 24h urine amount, urine protein, creatinine, blood urea nitrogen of rat and sees table 7.
Can know from the listed detected parameters comparisons such as kidney of respectively organizing rat of table 7: Chinese medicine of the present invention can effectively improve the early stage high filtration state of type 2 diabetes mellitus kidney, plays the effect of protection diabetes kidney, and is the strongest with the low dose group effect, is superior to traditional YIQITANGKANG group.
Table 7: each is organized rat kidney/body weight, 24h urine amount, urine protein, creatinine, blood urea nitrogen etc. and measures relatively (x ± s) of results
*P＜0.01, the vs normal group;
△P＜0.05,
△ △P＜0.01, the vs model group;
#P＜0.05,
##P＜0.01, vs YIQITANGKANG group
Six, Chinese medicine of the present invention is to the influence of type 2 diabetes mellitus rat cataract pathological changes
Animal experiment method is the same.Each eye of organizing rat carries out the view that observation post takes and sees Fig. 3 before platycoria; Each eye of organizing rat carries out the view that observation post takes with operating microscope and sees Fig. 4 behind platycoria.
Can know from Fig. 3, the eye state contrast of respectively organizing rat shown in Figure 4: the crystalline lens of model group rat occurs obviously muddy; And other are respectively organized rat and this variation do not occurred; Explain that the diabetic cataract pathological changes has taken place the model group rat, and the intervention medicine of high, medium and low dose groups of the present invention and YIQITANGKANG group all has certain preventive and therapeutic effect to type 2 diabetes mellitus rat cataract pathological changes.
Claims (8)
1. the hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism is characterized in that: process this Chinese medicine raw materials of effective components and consist of by weight ratio: 1.875～7.5 parts of the Radixs Astragali, 2.25～9.0 parts of Herb Gynostemmae Pentaphylli, 1.875～7.5 parts of Radix Puerariaes, 1.5～6.0 parts on Folium Mori, 1.5～6.0 parts of Semen Trigonellaes, 0.75～3.0 part of Rhizoma Coptidis, 0.375～1.5 part of Cortex Cinnamomi; Its preparation method comprises the steps:
1) get Rhizoma Coptidis and Radix Puerariae by above-mentioned weight proportion, mix homogeneously adopts the ethanol gradation to extract, merge extractive liquid,, and decompression recycling ethanol obtains Rhizoma Coptidis kudzu vine root mixed extract to there not being the alcohol flavor;
2) get Cortex Cinnamomi by above-mentioned weight proportion, be broken into coarse powder, adopt steam distillation to extract, obtain Cortex Cinnamomi volatile oil and Cortex Cinnamomi residue water liquid;
3) get Semen Trigonellae, the Radix Astragali, Herb Gynostemmae Pentaphylli and Folium Mori by above-mentioned weight proportion; Add step 2) gained Cortex Cinnamomi residue water liquid, adopt the distilled water gradation to decoct collecting decoction; Concentrating under reduced pressure; Add step 1) gained Rhizoma Coptidis kudzu vine root mixed extract and step 2 again) the gained Cortex Cinnamomi volatile oil, vacuum drying is made into powder, granule, tablet or capsule by conventional method at last.
2. the hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism according to claim 1 is characterized in that: process this Chinese medicine raw materials of effective components and consist of by weight ratio: 1.875～3.75 parts of the Radixs Astragali, 2.25～4.5 parts of Herb Gynostemmae Pentaphylli, 1.875～3.75 parts of Radix Puerariaes, 1.5～3.0 parts on Folium Mori, 1.5～3.0 parts of Semen Trigonellaes, 0.75～1.5 part of Rhizoma Coptidis, 0.375～0.75 part of Cortex Cinnamomi.
3. the hypoglycemic fat-regulating Chinese medicine with overall regulation of body metabolism according to claim 1 is characterized in that: process this Chinese medicine raw materials of effective components and consist of by weight ratio: 1.875 parts of the Radixs Astragali, 2.25 parts of Herb Gynostemmae Pentaphylli, 1.875 parts of Radix Puerariaes, 1.5 parts on Folium Mori, 1.5 parts of Semen Trigonellaes, 0.75 part of Rhizoma Coptidis, 0.375 part of Cortex Cinnamomi.
4. according to claim 1 or 2 or 3 described hypoglycemic fat-regulating Chinese medicines with overall regulation of body metabolism, it is characterized in that: the Rhizoma Coptidis raw material of processing pharmaceutically active ingredient in this adopts Rhizoma Coptidis.
5. according to claim 1 or 2 or 3 described hypoglycemic fat-regulating Chinese medicines with overall regulation of body metabolism, it is characterized in that: the dosage form of this Chinese medicine is a kind of in powder, granule, tablet or the capsule.
6. said hypoglycemic fat-regulating preparation method of Chinese medicine with overall regulation of body metabolism of claim 5, it is characterized in that: this method comprises the steps:
1) get Rhizoma Coptidis and Radix Puerariae by above-mentioned weight proportion, mix homogeneously adopts the ethanol gradation to extract, merge extractive liquid,, and decompression recycling ethanol obtains Rhizoma Coptidis kudzu vine root mixed extract to there not being the alcohol flavor;
2) get Cortex Cinnamomi by above-mentioned weight proportion, be broken into coarse powder, adopt steam distillation to extract, obtain Cortex Cinnamomi volatile oil and Cortex Cinnamomi residue water liquid;
3) get Semen Trigonellae, the Radix Astragali, Herb Gynostemmae Pentaphylli and Folium Mori by above-mentioned weight proportion; Add step 2) gained Cortex Cinnamomi residue water liquid, adopt the distilled water gradation to decoct collecting decoction; Concentrating under reduced pressure; Add step 1) gained Rhizoma Coptidis kudzu vine root mixed extract and step 2 again) the gained Cortex Cinnamomi volatile oil, vacuum drying is made into powder, granule, tablet or capsule by conventional method at last.
7. according to the said hypoglycemic fat-regulating preparation method of Chinese medicine of claim 6 with overall regulation of body metabolism; It is characterized in that: in the said step 1); Adopting concentration of volume percent is 70～75% ethanol extraction three times; Extraction time is 2.5～3.0 hours for the first time, and extraction time is 1.5～2.0 hours for the second time, and extraction time is 1.0～1.5 hours for the third time.
8. according to claim 6 or 7 said hypoglycemic fat-regulating preparation method of Chinese medicine with overall regulation of body metabolism; It is characterized in that: in the said step 3); Adopt the distillation water boiling and extraction three times; Decocting time is 2.0～2.5 hours for the first time, and decocting time is 1.0～1.5 hours for the second time, and decocting time is 1.0～1.5 hours for the third time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810048729 CN101336974B (en) | 2008-08-07 | 2008-08-07 | Traditional Chinese medicine for reducing blood sugar and regulating lipid with overall regulation of body metabolism and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810048729 CN101336974B (en) | 2008-08-07 | 2008-08-07 | Traditional Chinese medicine for reducing blood sugar and regulating lipid with overall regulation of body metabolism and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101336974A CN101336974A (en) | 2009-01-07 |
| CN101336974B true CN101336974B (en) | 2012-05-23 |
Family
ID=40211239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810048729 Expired - Fee Related CN101336974B (en) | 2008-08-07 | 2008-08-07 | Traditional Chinese medicine for reducing blood sugar and regulating lipid with overall regulation of body metabolism and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101336974B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016018799A3 (en) * | 2014-07-28 | 2016-03-24 | Nanzheng Cheng | Water extracts of cinnamon and radix astragali |
| US9877992B2 (en) | 2014-07-28 | 2018-01-30 | Nanzheng Cheng | Water extracts of cinnamon and Radix Astragali |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058652B (en) * | 2009-11-16 | 2013-04-24 | 甘肃中医学院 | Traditional Chinese medicinal extract for treating diabetes mellitus, medicinal composition thereof and preparation method thereof |
| CN103285227A (en) * | 2013-05-31 | 2013-09-11 | 浙江工业大学 | Hypoglycemic Chinese medicine composition, hypoglycemic tea and preparation method of hypoglycemic tea |
| CN114848748B (en) * | 2022-06-08 | 2023-04-28 | 河北御芝林生物科技有限公司 | Composition with blood sugar reducing effect and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238986A (en) * | 1999-06-24 | 1999-12-22 | 齐福东 | Health-care oral liquid for diabetes patients |
| CN1418689A (en) * | 2002-11-29 | 2003-05-21 | 张慧芳 | Medicine composition for treating diabetes, and its prepn. method |
| CN1692922A (en) * | 2005-04-11 | 2005-11-09 | 成都华高药业有限公司 | Medicine composite used for lowering blood-sugar, prepn. method and use thereof |
| CN1720982A (en) * | 2005-06-09 | 2006-01-18 | 陕西爱波卓科技有限责任公司 | Sugar decreasing composition of fenugreek seed |
-
2008
- 2008-08-07 CN CN 200810048729 patent/CN101336974B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1238986A (en) * | 1999-06-24 | 1999-12-22 | 齐福东 | Health-care oral liquid for diabetes patients |
| CN1418689A (en) * | 2002-11-29 | 2003-05-21 | 张慧芳 | Medicine composition for treating diabetes, and its prepn. method |
| CN1692922A (en) * | 2005-04-11 | 2005-11-09 | 成都华高药业有限公司 | Medicine composite used for lowering blood-sugar, prepn. method and use thereof |
| CN1720982A (en) * | 2005-06-09 | 2006-01-18 | 陕西爱波卓科技有限责任公司 | Sugar decreasing composition of fenugreek seed |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016018799A3 (en) * | 2014-07-28 | 2016-03-24 | Nanzheng Cheng | Water extracts of cinnamon and radix astragali |
| US9877992B2 (en) | 2014-07-28 | 2018-01-30 | Nanzheng Cheng | Water extracts of cinnamon and Radix Astragali |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101336974A (en) | 2009-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102727586B (en) | Composition for preventing and treating diabetes | |
| CN106074971A (en) | A kind of hypoglycemic traditional Chinese medicine composition and preparation method and application | |
| CN101336974B (en) | Traditional Chinese medicine for reducing blood sugar and regulating lipid with overall regulation of body metabolism and preparation method thereof | |
| CN115364170B (en) | Sugar metabolism regulator and preparation method and application thereof | |
| CN1299742C (en) | Medicine for treating diabetes, and its prepn. method | |
| KR20160007728A (en) | Method for manufacturing garlic skin extract and food composition for preventing and alleviating diabetes prepared using the same | |
| KR101094157B1 (en) | The health food for improvement of glucosuria using madisin-materials of plants | |
| CN102319289A (en) | Hyperglycemic Chinese medicinal composition comprising cinnamon polyphenol and the like and preparation method of hyperglycemic Chinese medicinal composition | |
| CN101336965B (en) | Chinese prepared medicine for improving sugar tolerance and reducing blood sugar and preparation method thereof | |
| CN113730464A (en) | New application of rhizoma coptidis pill, extract and pharmaceutical composition thereof and rhizoma coptidis pill product | |
| CN101167781A (en) | Orally-administered hypoglycemic sweet potato leaf single prescription traditional Chinese medicine and preparation method thereof | |
| EP1369123A1 (en) | A health-care product comprising lotus rhizome and process for its preparation | |
| CN109223811B (en) | Ginsenoside composition with hypoglycemic activity | |
| CN106692742A (en) | Traditional Chinese compound medicine for regulating blood sugar and preparation method of traditional Chinese compound medicine | |
| CN1618335A (en) | Health-care food with assistant functions of decreasing blood-sugar and blood-fat, and its prepn. method | |
| Benariba et al. | Antihyperglycemic effect of Citrullus colocynthis seed aqueous extracts in streptozotocin-induced diabetic rats | |
| CN100534461C (en) | Pharmaceutical composition for treating diabetes and impaired glucose tolerance and preparation method thereof | |
| CN103272146B (en) | Medicine composition used for preventing and treating alcoholic fatty liver and preparation method thereof | |
| KR20140032961A (en) | Composition for preventing and treating diabetes and diabetes complications comprising amphicarpaea edgeworthii var. trisperma powder or an extract thereof | |
| CN101406576B (en) | Chinese medicine for treating diabetes and preparation method | |
| CN106942439B (en) | Cyclocarya paliurus and guava leaf blood glucose reducing tea and preparation method and application thereof | |
| KR20100111088A (en) | Composition for preventing and treating diabetes mellitus or diabetic complications comprising extract of herbal combination | |
| CN101167887A (en) | Orally-administered matrimony vine single preparation traditional Chinese medicine for treating diabetes and preparation method thereof | |
| CN109966283A (en) | Application of the degreasing cinnamon polyphenol extract in preparation prevention and treatment diabetic nephropathy product | |
| CN115779046B (en) | Traditional Chinese medicine composition, traditional Chinese medicine preparation and application for preventing and treating diabetes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120523 Termination date: 20180807 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |