CN115932130A

CN115932130A - Online enrichment treatment system and application method thereof

Info

Publication number: CN115932130A
Application number: CN202211651906.0A
Authority: CN
Inventors: 刘艳; 王建凤; 冯月超; 祖文川; 吴赞; 陈婷; 姜其立
Original assignee: Analysis And Testing Institute Of Beijing Academy Of Science And Technology Beijing Physical And Chemical Analysis And Testing Center
Current assignee: Analysis And Testing Institute Of Beijing Academy Of Science And Technology Beijing Physical And Chemical Analysis And Testing Center
Priority date: 2022-12-21
Filing date: 2022-12-21
Publication date: 2023-04-07

Abstract

The invention relates to the technical field of pollutant detection, and provides an online enrichment treatment system and an application method thereof, wherein the treatment system comprises two pump bodies, a first six-way valve, a second six-way valve and an enrichment column; the first six-way valve is in fluid communication with the second six-way valve, one pump body is in fluid communication with the first six-way valve, the other pump body is in fluid communication with the second six-way valve, and the inlet end and the outlet end of the enrichment column are both in fluid communication with the second six-way valve; a quantitative ring is arranged between two interfaces of the first six-way valve, the first six-way valve is switched to a first state, the second six-way valve is correspondingly positioned at a first position, and a sample is injected into the quantitative ring; the first six-way valve is switched to a second state, the second six-way valve is correspondingly positioned at a second position, and a pump body is used for conveying a mobile phase to flush the quantitative ring, so that the sample is enriched to the enrichment column; the state of the first six-way valve is kept unchanged, the second six-way valve is correspondingly positioned at the first position, and the other pump body is used for conveying eluent to elute the enrichment column for online measurement, so that the detection of small sample amount is realized.

Description

Online enrichment treatment system and application method thereof

Technical Field

The invention relates to the technical field of pollutant detection, in particular to an online enrichment treatment system and an application method thereof.

Background

With the development of industries such as mining, metallurgy and the like, industrial wastewater related to pollutants is discharged into a water environment in large quantity, and the pollution event of a regional environmental water body by the pollutants occurs frequently. And pollutants can be remarkably enriched in human tissues and organs through a food chain, and when the intake of the pollutants in a human body exceeds a certain limit value, great harm can be caused to life health. Therefore, the development of daily monitoring of pollutants in the environmental water body is significant.

Because the pollutant content in the environmental water body is low, in order to meet the detection limit requirement, the low-concentration pollutant solution needs to be enriched and concentrated and then detected, generally, an adsorption material with a pollutant adsorption function is filled into a cylinder to form an enrichment column, when the low-concentration pollutant solution flows through the enrichment column, the pollutant is adsorbed by the adsorption material, a small amount of eluent is used for eluting pollutant elements on the adsorption material, and the high-concentration pollutant solution is obtained to realize detection.

However, the existing online enrichment system has many device components and a complex structure, does not form an integrated controllable device, and cannot accurately detect the amount of a small sample.

In view of this, the present invention is proposed.

Disclosure of Invention

The invention provides an online enrichment treatment system, which is used for solving the defects of complex structure and low detection accuracy of small sample amount of an online enrichment system in the prior art and realizing the online enrichment accurate detection of the small sample amount.

The invention also provides an application method of the online enrichment treatment system.

The invention provides an online enrichment treatment system which comprises two pump bodies, a first six-way valve, a second six-way valve and an enrichment column, wherein the two pump bodies are connected with the enrichment column through a pipeline;

the first and second six-way valves are in fluid communication, one of the pump bodies being in fluid communication with the first six-way valve, the other pump body being in fluid communication with the second six-way valve, both the inlet and outlet ends of the enrichment column being in fluid communication with the second six-way valve;

when the first six-way valve is switched to a first state and the second six-way valve is correspondingly positioned at a first position, a sample is fed into the quantitative ring; when the first six-way valve is switched to a second state and the second six-way valve is correspondingly positioned at a second position, a pump body in fluid communication with the first six-way valve transmits a mobile phase to flush the quantitative ring, so that the sample is enriched to the enrichment column; and when the state of the first six-way valve is kept unchanged and the second six-way valve is correspondingly positioned at the first position, a pump body in fluid communication with the second six-way valve transmits eluent to elute the enrichment column for online measurement.

According to the online enrichment treatment system provided by the invention, the 1# interface of the first six-way valve is connected with a sample injection device, and the 2# interface is connected with waste liquid;

when the first six-way valve is switched to the first state, the 6# interface of the first six-way valve is communicated with the 1# interface, the 3# interface is communicated with the 2# interface, the quantitative ring is arranged between the 3# interface and the 6# interface, the 5# interface is communicated with one of the pump bodies, and the 4# interface is communicated with the second six-way valve;

when the first six-way valve is switched to the second state, the 6# interface of the first six-way valve is communicated with the 5# interface, the 3# interface is communicated with the 4# interface, the 1# interface is communicated with the 2# interface, the quantitative ring is arranged between the 3# interface and the 6# interface, the 5# interface is communicated with one of the pump bodies, and the 4# interface is communicated with the second six-way valve.

According to the online enrichment treatment system provided by the invention, the 6# interface of the second six-way valve is communicated with the first six-way valve, the 5# interface is connected with waste liquid, and the 2# interface is connected with detection equipment;

when the second six-way valve is switched to the first state, the 1# interface of the second six-way valve is communicated with the 6# interface, the 4# interface of the second six-way valve is communicated with the 5# interface, the enrichment column is arranged between the 1# interface and the 4# interface, the 3# interface is communicated with the other pump body, and the 2# interface is communicated with the 3# interface;

when the second six-way valve is switched to the second state, the 1# interface of the second six-way valve is communicated with the 2# interface, the 3# interface of the second six-way valve is communicated with the 4# interface, the enrichment column is arranged between the 1# interface and the 4# interface, the 3# interface is communicated with the other pump body, and the 5# interface is communicated with the 6# interface.

The invention also provides an application method of the online enrichment treatment system, which comprises the following steps:

a sample is fed into the quantitative ring through a first six-way valve;

starting a pump body in fluid communication with the first six-way valve to deliver mobile phase to flush the dosing ring to enrich the sample to the enrichment column;

and starting a pump body in fluid communication with the second six-way valve to convey eluent to elute the enrichment column, wherein the pump body is suitable for carrying out online measurement on a sample with the sample volume less than 15 ml.

The invention also provides an online enrichment treatment system, which comprises two pump bodies, a first six-way valve, a second six-way valve, a third six-way valve, a first enrichment column and a second enrichment column;

the first and second six-way valves are in fluid communication, with one pump body in fluid communication with the first six-way valve, with the other pump body in fluid communication with the third six-way valve, the inlet port of the first enrichment column in fluid communication with the third six-way valve, the outlet port of the first enrichment column in fluid communication with the second six-way valve, the inlet port of the second enrichment column in fluid communication with the second six-way valve, the outlet port of the second enrichment column in fluid communication with the third six-way valve;

when the second six-way valve is in a first position and the third six-way valve is in a third position, the pump body in fluid communication with the first six-way valve conveys a second sample to the second enrichment column and the pump body in fluid communication with the third six-way valve conveys an eluent to elute the first sample of the first enrichment column for on-line measurement;

when the second six-way valve is located at a second position and the third six-way valve is located at a fourth position, the pump body in fluid communication with the first six-way valve conveys a first sample to be enriched to the first enrichment column, and the pump body in fluid communication with the third six-way valve conveys an eluent to elute a second sample of the second enrichment column for on-line measurement.

According to the online enrichment treatment system provided by the invention, a quantitative ring is arranged between two interfaces of the first six-way valve;

when the first six-way valve is switched to a first state, the second six-way valve is correspondingly positioned at a first position, and the third six-way valve is correspondingly positioned at a third position, the sample is fed into the quantitative ring;

when the first six-way valve is switched to a second state, the second six-way valve is correspondingly positioned at a second position, and the third six-way valve is correspondingly positioned at a fourth position, the pump body which is in fluid communication with the first six-way valve transmits a mobile phase to flush the quantitative ring, so that a sample is enriched to the first enrichment column;

when the first six-way valve is switched to a first state, the second six-way valve is correspondingly positioned at a first position, and the third six-way valve is correspondingly positioned at a third position, a pump body which is in fluid communication with the second six-way valve is used for conveying eluent to elute the first enrichment column so as to carry out online determination, and meanwhile, a second sample is injected into a quantitative ring;

when the first six-way valve is switched to a second state, the second six-way valve is correspondingly positioned at a first position, and the third six-way valve is correspondingly positioned at a third position, the pump body which is in fluid communication with the first six-way valve transmits a mobile phase to flush the quantitative ring, so that a second sample is enriched to the second enrichment column;

when the state of the first six-way valve is kept unchanged, the second six-way valve is correspondingly positioned at the second position, and the third six-way valve is correspondingly positioned at the fourth position, the pump body which is in fluid communication with the first six-way valve transmits a mobile phase to flush the quantitative ring, so that a first sample is enriched to the first enrichment column, and the pump body which is in fluid communication with the third six-way valve transmits eluent to elute the second enrichment column for online measurement.

According to the online enrichment processing system provided by the invention, the 5# interface of the two six-way valve is communicated with the first six-way valve, and the 2# interface is connected with the detection equipment;

when the second six-way valve is located at a first position, a 6# interface of the second six-way valve is communicated with a 5# interface, a 4# interface of the second six-way valve is communicated with a 6# interface, a 3# interface of the second six-way valve is communicated with a 4# interface, a 2# interface of the second six-way valve is communicated with a 1# interface, an inlet end of the second enrichment column is communicated with a 3# interface of the second six-way valve, an outlet end of the second enrichment column is communicated with the third six-way valve, and the 1# interface of the second six-way valve is communicated with an outlet end of the first enrichment column;

when the second six-way valve is located at the second position, the 1# interface and the 6# interface of the second six-way valve are communicated, the 6# interface and the 4# interface are communicated, the 4# interface and the 5# interface are communicated, the 2# interface and the 3# interface are communicated, the outlet end of the second enrichment column is communicated with the 3# interface of the second six-way valve, the inlet end of the second enrichment column is communicated with the third six-way valve, and the 1# interface is communicated with the inlet end of the first enrichment column.

According to the online enrichment treatment system provided by the invention, the 2# interface of the third six-way valve is communicated with one of the pump bodies, and the 5# interface is connected with waste liquid;

when the third six-way valve is located at a third position, a 1# interface of the third six-way valve is communicated with a 2# interface, a 3# interface of the third six-way valve is communicated with a 4# interface, the 4# interface is communicated with a 6# interface, the 6# interface is communicated with a 5# interface, an outlet end of the second enrichment column is communicated with the 3# interface, and an inlet end of the first enrichment column is communicated with the 1# interface;

when the third six-way valve is located at the fourth position, the 2# interface of the third six-way valve is communicated with the 3# interface, the 1# interface is communicated with the 6# interface, the 6# interface is communicated with the 4# interface, the 4# interface is communicated with the 5# interface, the inlet end of the second enrichment column is communicated with the 3# interface, and the outlet end of the first enrichment column is communicated with the 1# interface.

feeding a first sample to a quantitative ring through a first six-way valve;

actuating a pump body in fluid communication with the first six-way valve to deliver mobile phase to flush the dosing ring to enrich the first sample to a first enrichment column;

starting a pump body in fluid communication with the third six-way valve to convey eluent to elute the first enrichment column, wherein the first enrichment column is suitable for carrying out online measurement on a first sample with the sample volume less than 15ml, and meanwhile, a second sample is injected to the quantitative ring through the first six-way valve;

starting a pump body in fluid communication with the first six-way valve to deliver mobile phase to flush the dosing ring to enrich the second sample to a second enrichment column;

starting a pump body which is in fluid communication with the third six-way valve to convey eluent to elute the second enrichment column, and carrying out online measurement on a second sample with the sample volume less than 15ml, wherein the first sample is injected into the quantitative ring through the first six-way valve;

and the steps are circulated.

According to the online enrichment processing system provided by the invention, the quantitative ring is arranged between the two interfaces of the first six-way valve, when the first six-way valve is switched to the first state and the second six-way valve is correspondingly positioned at the first position, a sample is fed to the quantitative ring, the quantitative ring can accurately quantify the sample amount, and the accurate amount is realized, so that the online enrichment processing system can accurately detect the online enrichment of small sample amounts.

Compared with the prior art, the online enrichment treatment system provided by the invention also realizes online elution and enrichment detection by switching the first state and the second state of the first six-way valve, switching the first position and the second position of the second six-way valve and communicating the two six-way valves, and has a simple structure.

Drawings

In order to more clearly illustrate the present invention or the technical solutions in the prior art, the drawings used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a schematic structural diagram of an on-line enrichment treatment system provided in example 1 of the present invention in a first state;

FIG. 2 is a schematic structural diagram of an on-line enrichment treatment system provided in example 1 of the present invention in a second state;

FIG. 3 is a schematic structural diagram of an on-line enrichment treatment system provided in example 1 of the present invention in a third state;

FIG. 4 is a schematic structural diagram of the on-line enrichment treatment system provided in embodiment 2 of the invention with the second six-way valve in the first position;

FIG. 5 is a schematic diagram of the configuration of the second six-way valve of the on-line enrichment processing system provided by the embodiment 2 of the invention at the second position;

FIG. 6 is a schematic structural diagram of the on-line enrichment treatment system provided in embodiment 3 of the invention with the second six-way valve in the first position and the third six-way valve in the third position;

FIG. 7 is a schematic structural diagram of the on-line enrichment treatment system provided in embodiment 3 of the invention, wherein the second six-way valve is located at the second position and the third six-way valve is located at the fourth position;

FIG. 8 is a schematic structural diagram of the on-line enrichment treatment system provided in example 4 of the present invention with the first six-way valve in the first position, the second six-way valve in the third position, and the third six-way valve in the fifth position;

FIG. 9 is a schematic structural diagram of the on-line enrichment treatment system provided in example 4 of the invention with the first six-way valve in the second position, the second six-way valve in the fourth position and the third six-way valve in the sixth position;

FIG. 10 is a schematic structural diagram of the on-line enrichment treatment system provided in example 4 of the present invention with the first six-way valve in the first position, the second six-way valve in the third position, and the third six-way valve in the fifth position;

FIG. 11 is a schematic structural diagram of the on-line enrichment treatment system provided in example 4 of the present invention with the first six-way valve in the second position, the second six-way valve in the third position, and the third six-way valve in the fifth position;

FIG. 12 is a schematic structural diagram of the on-line enrichment treatment system provided in example 4 of the invention with the first six-way valve in the second position, the second six-way valve in the fourth position and the third six-way valve in the sixth position;

FIG. 13 is one of the schematic block diagrams of the controller of the on-line enrichment processing system provided by the present invention;

FIG. 14 is a second schematic diagram of the controller of the on-line enrichment processing system according to the present invention.

Reference numerals are as follows:

100. a first pump body; 200. a second pump body; 300. a first six-way valve;

400. a second six-way valve; 500. a first enrichment column; 600. a dosing ring;

700. a third six-way valve; 800. a second enrichment column;

11. a programmable logic controller; 111. a power supply module; 112. a central processing unit; 113. a storage module; 114. an input module; 115. an output module; 116. and a peripheral interface module.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. Specific meanings of the above terms in the embodiments of the present invention can be understood in specific cases by those of ordinary skill in the art.

In embodiments of the invention, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of an embodiment of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

An embodiment of the present invention will be described below with reference to fig. 1 to 14. It should be understood that the following description is only exemplary embodiments of the present invention and is not intended to limit the present invention in any way.

Example 1

Referring to fig. 1 to 3, the on-line enrichment treatment system includes a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, and a first enrichment column 500, wherein the first pump body 100 and the second pump body 200 are both high-pressure pumps;

the first pump 100 is in fluid communication with the first six-way valve 300, the second pump 200 is in fluid communication with the second six-way valve 400, the first six-way valve 300 is in fluid communication with the second six-way valve 400, and both the inlet and outlet ends of the first enrichment column 500 are in fluid communication with the second six-way valve 400;

wherein a dosing ring 600 is provided between the two interfaces of the first six-way valve 300, the first six-way valve 300 being adapted to switch between a first state and a second state, the second six-way valve 400 being adapted to switch between a first position and a second position.

In the first state, as shown in fig. 1, the 1# port of the first six-way valve 300 communicates with the 6# port, the 2# port communicates with the 3# port, the 4# port communicates with the 5# port, and the dosing ring 600 is connected between the 3# port and the 6# port.

At this time, the second six-way valve 400 is in the first position, and the # 1 port of the second six-way valve 400 communicates with the # 2 port, the # 3 port communicates with the # 4 port, and the # 5 port communicates with the # 6 port.

In the second state, as shown in fig. 2, the 1# port of the first six-way valve 300 communicates with the 2# port, the 3# port communicates with the 4# port, the 5# port communicates with the 6# port, and the dosing ring 600 is connected between the 3# port and the 6# port.

At this time, the second six-way valve 400 is in the second position, and the # 1 port of the second six-way valve 400 communicates with the # 6 port, the # 2 port communicates with the # 3 port, and the # 4 port communicates with the # 5 port.

Referring to fig. 1, specifically, when the first six-way valve 300 is in the first state, the # 1 interface of the first six-way valve 300 is connected to the sample injection device, and the # 2 interface of the first six-way valve 300 is connected to the waste liquid; the # 6 port of the first six-way valve 300 communicates with the # 1 port of the first six-way valve 300, and the # 5 port of the first six-way valve 300 communicates with the first pump body 100; the 3# interface of the first six-way valve 300 is communicated with the 2# interface of the first six-way valve 300, the quantitative ring 600 is arranged between the 3# interface of the first six-way valve 300 and the 6# interface of the first six-way valve 300, a sample can enter from the 1# interface of the first six-way valve 300 and enter the quantitative ring 600 through the 6# interface for quantitative sample injection, and the quantitative ring 600 can accurately sample and is suitable for sampling samples with the sample amount less than 15 ml.

Correspondingly, the second six-way valve 400 is located at the first position, the interface 6# of the second six-way valve 400 is communicated with the interface 5# of the second six-way valve 400, the interface 5# of the second six-way valve 400 is connected with the waste liquid, and the interface 6# of the second six-way valve 400 is communicated with the interface 4# of the first six-way valve 300. In the first condition, neither the first pump 100 nor the second pump 200 is active.

Referring to fig. 2, when the first six-way valve 300 is switched to the second state, the second pump body 200 does not operate, the # 1 interface of the first six-way valve 300 is connected to the sample injection device, the # 2 interface of the first six-way valve 300 is connected to the waste liquid, the # 1 interface of the first six-way valve 300 is communicated with the # 2 interface of the first six-way valve 300, the # 6 interface of the first six-way valve 300 is communicated with the # 5 interface of the first six-way valve 300, the # 3 interface of the first six-way valve 300 is communicated with the # 4 interface of the first six-way valve 300, the quantitative ring 600 is disposed between the # 3 interface of the first six-way valve 300 and the # 6 interface of the first six-way valve 300, the # 5 interface of the first six-way valve 300 is communicated with the first pump body 100, and the # 4 interface of the first six-way valve 300 is communicated with the # 6 interface of the second six-way valve 400, so that the first pump body 100 delivers mobile phase to wash the quantitative ring 600, and enrich the sample to the first enrichment column 500.

Correspondingly, the second six-way valve 400 is located at the second position, the # 1 interface of the second six-way valve 400 is communicated with the # 6 interface of the second six-way valve 400, the # 4 interface of the second six-way valve 400 is communicated with the # 5 interface of the second six-way valve 400, the first enrichment column 500 is arranged between the # 1 interface of the second six-way valve 400 and the # 4 interface of the second six-way valve 400, the # 3 interface of the second six-way valve 400 is communicated with the second pump body 200, the # 2 interface of the second six-way valve 400 is communicated with the # 3 interface of the second six-way valve 400, and the # 2 interface of the second six-way valve 400 is connected with the detection device.

In the second state, after the first pump body 100 conveys the mobile phase to flush the quantitative ring 600, the fluid flows into the inflow end of the first enrichment column 500 through the # 6 port of the second six-way valve 400 and the # 1 port of the second six-way valve 400, and after the fluid is enriched by the first enrichment column 500, the fluid flows into the # 4 port of the second six-way valve 400 from the outflow end of the first enrichment column 500, and finally the waste fluid is discharged through the # 5 port of the second six-way valve 400, thereby completing the enrichment of the sample.

Referring to FIG. 3, the first six-way valve 300 remains in the second state and, correspondingly, the second six-way valve 400 is in the first position. The first pump body 100 does not work, the # 1 interface of the second six-way valve 400 is communicated with the # 2 interface of the second six-way valve 400, the # 3 interface of the second six-way valve 400 is communicated with the # 4 interface of the second six-way valve 400, the first enrichment column 500 is arranged between the # 1 interface of the second six-way valve 400 and the # 4 interface of the second six-way valve 400, the # 3 interface of the second six-way valve 400 is communicated with the second pump body 200, the # 2 interface of the second six-way valve 400 is connected with a detection device, and the # 5 interface of the second six-way valve 400 is communicated with the # 6 interface of the second six-way valve 400.

In the second state, the second pump body 200 delivers the eluent to flush the first enrichment column 500, the fluid flows into the inflow end of the first enrichment column 500 through the 3# interface of the second six-way valve 400 and the 4# interface of the second six-way valve 400, after the sample enriched by the first enrichment column 500 is eluted, the fluid flows into the 1# interface of the second six-way valve 400 from the outflow end of the first enrichment column 500, and finally flows into the detection device through the 2# interface of the second six-way valve 400, so that the elution and the online detection of the sample are completed.

Based on the above online enrichment treatment system, the invention also provides an application method of the online enrichment treatment system, which comprises the following steps:

a sample is fed into the quantitative ring 600 through the first six-way valve 300;

activating a pump body in fluid communication with the first six-way valve 300 to deliver mobile phase to flush the dosing ring 600 to enrich the sample to the enrichment column 500;

the pump body in fluid communication with the second six-way valve 400 is actuated to deliver the eluent to elute the enrichment column 500 and perform an online test, suitable for online testing of samples having a sample size of less than 15 ml.

Compared with the online enrichment processing system in the prior art, the online enrichment processing system structure disclosed by the invention has the advantages that the first six-way valve 300 is switched between the first state and the second state, and the second six-way valve 400 is switched between the first position and the second position, so that three states of the online enrichment processing system are formed, the accurate quantitative sampling of small samples is realized, the online enrichment and elution detection are realized, and the structure is simple.

Example 2

Referring to fig. 4-5, in some embodiments of the present invention, the on-line enrichment processing system includes a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, a third six-way valve 700, a first enrichment column 500;

referring to fig. 4, when the first and third six-way valves 300 and 700 are not operated and the second six-way valve 400 is located at the first position, the # 5 port of the second six-way valve 400 is connected to the waste liquid, the first pump body 100 is connected to the # 6 port of the second six-way valve 400, the # 1 port of the second six-way valve 400 is connected to the # 6 port of the second six-way valve 400, the # 2 port of the second six-way valve 400 is connected to the # 3 port of the second six-way valve 400, the second pump body 200 is connected to the # 3 port of the second six-way valve 400, the # 4 port of the second six-way valve 400 is connected to the # 5 port of the second six-way valve 400, one end of the first enrichment column 500 is connected to the # 1 port of the second six-way valve 400, the other end of the first enrichment column 500 is connected to the # 4 port of the second six-way valve 400, the first pump body 100 is activated, and the sample is enriched to the first enrichment column 500 through the # 6 port of the second six-way valve 400 and the # 4 port of the waste liquid outlet port of the second six-way valve 400.

Referring to fig. 5, when the first and third six-way valves 300 and 700 are not operated, and the second six-way valve 400 is located at the second position, the # 1 port of the second six-way valve 400 is communicated with the # 2 port of the second six-way valve 400, the # 3 port of the second six-way valve 400 is communicated with the # 4 port of the second six-way valve 400, the # 5 port of the second six-way valve 400 is communicated with the # 6 port of the second six-way valve 400, the first pump body 100 is connected with the # 6 port of the second six-way valve 400, the # 5 port of the second six-way valve 400 is connected with the waste liquid, the # 2 port of the second six-way valve 400 is connected with the detection device, the second pump body 200 is activated, the eluent is pumped to the # 1 port of the second six-way valve 400 through the # 3 port and the # 4 port of the second six-way valve 400, one end of the first enrichment column 500 is connected with the # 4 port of the second six-way valve 400, the other end of the enrichment column 500 is connected with the # 1 port of the second six-way valve 400, and the eluent flows into the first enrichment column 500, and flows into the detection device 500 through the # 2 port, and the first enrichment column 500, and the detection device 500, and elutes the eluent to complete the eluent flowing into the detection device 400. The embodiment can complete on-line detection by using the six-way valve, is suitable for single sample enrichment and is suitable for large-volume samples.

Example 3

Referring to fig. 6 to 7, the on-line enrichment processing system includes a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, a third six-way valve 700, a first enrichment column 500, and a second enrichment column 800;

the first pump body 100 is in fluid communication with the first six-way valve 300, the second pump body 200 is in fluid communication with the third six-way valve 700, the first six-way valve 300 is in fluid communication with the second six-way valve 400, both ends of the first enrichment column 500 are in fluid communication with the second six-way valve 400 and the third six-way valve 700, respectively, both ends of the second enrichment column 800 are in fluid communication with the second six-way valve 400 and the third six-way valve 700, respectively;

wherein in the first six-way valve 300 position, the second six-way valve 400 is adapted to switch between the first position and the second position, and the third six-way valve 700 is adapted to switch between the third position and the fourth position;

referring to fig. 6, the first six-way valve 300 is unchanged in position, the second six-way valve 400 is located in the first position, the third six-way valve 700 is located in the third position, the first pump body 100 conveys mobile phase to wash the second enrichment column 800 after passing through the first six-way valve 300 and the second six-way valve 400, so that the sample is enriched to the second enrichment column 800, and meanwhile, the second pump body 200 conveys eluent to elute the first enrichment column 500 after passing through the third six-way valve 700, so as to complete online detection.

Referring to fig. 7, the position of the first six-way valve 300 is unchanged, the second six-way valve 400 is located at the second position, the third six-way valve 700 is located at the fourth position, the first pump 100 conveys mobile phase to pass through the first six-way valve 300 and the second six-way valve 400 and then wash the first enrichment column 500, so that the sample is enriched to the first enrichment column 500, and meanwhile, the second pump 200 conveys eluent to pass through the third six-way valve 700 and then elute the second enrichment column 800, so as to complete online detection.

In the embodiment, the first six-way valve 300 is fixed, and the second six-way valve 400 and the third six-way valve 700 are fixed, so that online enrichment detection of two samples can be realized, and quantitative loop sample injection is not needed, so that the method is suitable for large-volume samples.

It can be understood that, referring to fig. 6, when the first six-way valve 300 is not changed in position, the second six-way valve 400 is located in the first position, and the third six-way valve 700 is located in the third position, the # 1 port of the first six-way valve 300 is communicated with the # 6 port of the first six-way valve 300, the # 2 port of the first six-way valve 300 is communicated with the # 3 port of the first six-way valve 300, both ends of the dosing ring 600 are respectively connected with the # 3 port and the # 6 port of the first six-way valve 300, the # 4 port of the first six-way valve 300 is communicated with the # 5 port of the first six-way valve 300, the # 5 port of the first six-way valve 300 is communicated with the first pump body 100, the # 4 port of the first six-way valve 300 is communicated with the # 5 port of the second six-way valve 400, the # 6 port of the second six-way valve 400 is communicated with the # 5 port of the second six-way valve 400, the # 4 port of the second six-way valve 400 is communicated with the # 6 port of the second six-way valve 400, the # 3 port of the second six-way valve 400 is connected with the inlet port of the second enrichment column 800, the outlet port of the second enrichment column 800 is connected with the # 3 port of the third six-way valve 700, the # 4 port of the third six-way valve 700 is communicated with the # 3 port of the third six-way valve 700, the # 6 port of the third six-way valve 700 is communicated with the # 4 port of the third six-way valve 700, the # 5 port of the third six-way valve 700 is communicated with the # 6 port of the third six-way valve 700, the # 5 port of the third six-way valve 700 is connected with the waste liquid, the # 2 port of the third six-way valve 700 is connected with the second pump body 200, the # 1 port of the third six-way valve 700 is communicated with the # 2 port of the third six-way valve 700, the inlet port of the first enrichment column 500 is connected with the # 1 port of the third six-way valve 700, the outlet port of the first enrichment column 500 is connected with the # 1 port of the second six-way valve 400, and the # 2 port of the second six-way valve 400 is communicated with the # 1 port of the second six-way valve 400, the # 2 interface of the second six-way valve 400 is connected to the detection device.

The first pump 100 transmits the mobile phase to flow into the 5# port of the second six-way valve 400 through the 5# port of the first six-way valve 300 and the 4# port of the first six-way valve 300, then flows into the second enrichment column 800 after flowing through the 6# port of the second six-way valve 400, the 4# port of the second six-way valve 400 and the 3# port of the second six-way valve 400, so that the sample is enriched in the second enrichment column 800, and the waste liquid flows into the 3# port of the third six-way valve 700, the 4# port of the third six-way valve 700, the 6# port of the third six-way valve 700 and the 5# port of the third six-way valve 700 through the outlet end of the second enrichment column 800, and finally flows into waste liquid treatment.

The second pump body 200 transmits the eluent to flow into the first enrichment column 500 through the # 2 interface of the third six-way valve 700 and the # 1 interface of the third six-way valve 700, so as to elute the first enrichment column 500, and the eluent flows into the # 1 interface of the second six-way valve 400 and the # 2 interface of the second six-way valve 400, so as to complete the online detection.

Referring to fig. 7, when the first six-way valve 300 is not changed in position, the second six-way valve 400 is in the second position, and the third six-way valve 700 is in the fourth position, the 1# port of the first six-way valve 300 is communicated with the 6# port of the first six-way valve 300, the 2# port of the first six-way valve 300 is communicated with the 3# port of the first six-way valve 300, both ends of the dosing ring 600 are respectively connected with the 3# port and the 6# port of the first six-way valve 300, the 4# port of the first six-way valve 300 is communicated with the 5# port of the first six-way valve 300, the 5# port of the first six-way valve 300 is communicated with the first pump body 100, the 4# port of the first six-way valve 300 is communicated with the 5# port of the second six-way valve 400, the 4# port of the second six-way valve 400 is communicated with the 5# port of the second six-way valve 400, the 6# port of the second six-way valve 400 is communicated with the 4# port of the second six-way valve 400, a # 2 port of the second six-way valve 400 is communicated with a # 3 port of the second six-way valve 400, a # 1 port of the second six-way valve 400 is connected with an inlet port of the first enrichment column 500, an outlet port of the first enrichment column 500 is connected with a # 1 port of the third six-way valve 700, a # 6 port of the third six-way valve 700 is communicated with a # 1 port of the third six-way valve 700, a # 4 port of the third six-way valve 700 is communicated with a # 6 port of the third six-way valve 700, a # 5 port of the third six-way valve 700 is communicated with a # 4 port of the third six-way valve 700, a # 5 port of the third six-way valve 700 is connected with the waste liquid, a # 2 port of the third six-way valve 700 is connected with the second pump body 200, a # 3 port of the third six-way valve 700 is communicated with a # 2 port of the third six-way valve 700, an inlet port of the second enrichment column 800 is connected with a # 3 port of the third six-way valve 700, an outlet port of the second enrichment column 800 is connected with a # 3 port of the second six-way valve 400, the # 2 interface of the second six-way valve 400 is connected to the detection device.

The first pump 100 transfers the mobile phase to flow into the 5# port of the second six-way valve 400 through the 5# port of the first six-way valve 300 and the 4# port of the first six-way valve 300, then flows into the first enrichment column 500 after flowing through the 4# port of the second six-way valve 400, the 6# port of the second six-way valve 400 and the 1# port of the second six-way valve 400, so that the sample is enriched in the first enrichment column 500, and the waste liquid flows into the 1# port of the third six-way valve 700, the 6# port of the third six-way valve 700, the 4# port of the third six-way valve 700 and the 5# port of the third six-way valve 700 through the outlet end of the first enrichment column 500, and finally flows into waste liquid treatment.

The second pump body 200 transmits the eluent to flow into the second enrichment column 800 through the # 2 interface of the third six-way valve 700 and the # 3 interface of the third six-way valve 700, the second enrichment column 800 is eluted, and the eluent flows to the # 3 interface of the second six-way valve 400 and the # 2 interface of the second six-way valve 400, so that the online detection is completed.

Example 4

Referring to fig. 8 to 12, the on-line enrichment processing system includes a first pump body 100, a second pump body 200, a first six-way valve 300, a second six-way valve 400, a third six-way valve 700, a first enrichment column 500, and a second enrichment column 800;

the first pump body 100 is in fluid communication with the first six-way valve 300, the second pump body 200 is in fluid communication with the third six-way valve 700, the first six-way valve 300 is in fluid communication with the second six-way valve 400, the inlet end of the first enrichment column 500 is in fluid communication with the third six-way valve 700, the outlet end of the first enrichment column 500 is in fluid communication with the second six-way valve 400, the inlet end of the second enrichment column 800 is in fluid communication with the second six-way valve 400, and the outlet end of the second enrichment column 800 is in fluid communication with the third six-way valve 700;

wherein a dosing ring 600 is provided between the two connections of the first six-way valve 300, the first six-way valve 300 is adapted to be switched between a first position and a second position, the second six-way valve 400 is adapted to be switched between a third position and a fourth position, and the third six-way valve 700 is adapted to be switched between a fifth position and a sixth position;

referring to fig. 8, the first six-way valve 300 is located at the first position, the second six-way valve 400 is located at the third position, the third six-way valve 700 is located at the fifth position, the # 1 port of the first six-way valve 300 is communicated with the # 6 port of the first six-way valve 300, the # 2 port of the first six-way valve 300 is communicated with the # 3 port of the first six-way valve 300, the # 4 port of the first six-way valve 300 is communicated with the # 5 port of the first six-way valve 300, and two ends of the dosing ring 600 are respectively connected with the # 3 port and the # 6 port of the first six-way valve 300; the 1# port of the second six-way valve 400 is communicated with the 2# port of the second six-way valve 400, the 3# port of the second six-way valve 400 is communicated with the 4# port of the second six-way valve 400, the 4# port of the second six-way valve 400 is communicated with the 6# port of the second six-way valve 400, and the 6# port of the second six-way valve 400 is communicated with the 5# port of the second six-way valve 400; a # 1 interface of the third six-way valve 700 is communicated with a # 2 interface of the third six-way valve 700, a # 3 interface of the third six-way valve 700 is communicated with a # 4 interface of the third six-way valve 700, a # 4 interface of the third six-way valve 700 is communicated with a # 6 interface of the third six-way valve 700, a # 6 interface of the third six-way valve 700 is communicated with a # 5 interface of the third six-way valve 700, and a first sample is injected into the dosing ring 600;

referring to fig. 9, the first six-way valve 300 is located at the second position, the second six-way valve 400 is located at the fourth position, the third six-way valve 700 is located at the sixth position, the # 1 port of the first six-way valve 300 is communicated with the # 2 port of the first six-way valve 300, the # 3 port of the first six-way valve 300 is communicated with the # 4 port of the first six-way valve 300, the # 6 port of the first six-way valve 300 is communicated with the # 5 port of the first six-way valve 300, and two ends of the dosing ring 600 are respectively connected with the # 3 port and the # 6 port of the first six-way valve 300; the # 1 port of the second six-way valve 400 is communicated with the # 6 port of the second six-way valve 400, the # 4 port of the second six-way valve 400 is communicated with the # 6 port of the second six-way valve 400, the # 5 port of the second six-way valve 400 is communicated with the # 4 port of the second six-way valve 400, and the # 3 port of the second six-way valve 400 is communicated with the # 2 port of the second six-way valve 400; the 1# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 5# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 is communicated with the 2# interface of the third six-way valve 700, and the first pump body 100 conveys the mobile phase flushing quantitative ring 600 to enrich the first sample to the first enrichment column 500;

referring to fig. 10, the first six-way valve 300 is located at the first position, the second six-way valve 400 is located at the third position, the third six-way valve 700 is located at the fifth position, the # 1 port of the first six-way valve 300 is communicated with the # 6 port of the first six-way valve 300, the # 2 port of the first six-way valve 300 is communicated with the # 3 port of the first six-way valve 300, the # 4 port of the first six-way valve 300 is communicated with the # 5 port of the first six-way valve 300, and two ends of the dosing ring 600 are respectively connected with the # 3 port and the # 6 port of the first six-way valve 300; the 1# port of the second six-way valve 400 is communicated with the 2# port of the second six-way valve 400, the 3# port of the second six-way valve 400 is communicated with the 4# port of the second six-way valve 400, the 4# port of the second six-way valve 400 is communicated with the 6# port of the second six-way valve 400, and the 6# port of the second six-way valve 400 is communicated with the 5# port of the second six-way valve 400; the 1# interface of the third six-way valve 700 is communicated with the 2# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is communicated with the 5# interface of the third six-way valve 700, the second pump body 200 transmits eluent to elute the first enrichment column 500 to realize online measurement, and meanwhile, a second sample is injected into the quantitative ring 600;

referring to fig. 11, the first six-way valve 300 is located at the second position, the second six-way valve 400 is located at the third position, the third six-way valve 700 is located at the fifth position, the # 1 port of the first six-way valve 300 is communicated with the # 2 port of the first six-way valve 300, the # 3 port of the first six-way valve 300 is communicated with the # 4 port of the first six-way valve 300, the # 5 port of the first six-way valve 300 is communicated with the # 6 port of the first six-way valve 300, and two ends of the dosing ring 600 are respectively connected with the # 3 port and the # 6 port of the first six-way valve 300; the 1# port of the second six-way valve 400 is communicated with the 2# port of the second six-way valve 400, the 3# port of the second six-way valve 400 is communicated with the 4# port of the second six-way valve 400, the 4# port of the second six-way valve 400 is communicated with the 6# port of the second six-way valve 400, and the 6# port of the second six-way valve 400 is communicated with the 5# port of the second six-way valve 400; the 1# interface of the third six-way valve 700 is communicated with the 2# interface of the third six-way valve 700, the 3# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, the 6# interface of the third six-way valve 700 is communicated with the 5# interface of the third six-way valve 700, and the first pump body 100 conveys the mobile phase to flush the quantitative ring 600, so that the second sample is enriched to the second enrichment column 800;

referring to fig. 12, the first six-way valve 300 is located at the second position, the second six-way valve 400 is located at the fourth position, the third six-way valve 700 is located at the sixth position, the port # 1 of the first six-way valve 300 is communicated with the port # 2 of the first six-way valve 300, the port # 3 of the first six-way valve 300 is communicated with the port # 4 of the first six-way valve 300, the port # 6 of the first six-way valve 300 is communicated with the port # 5 of the first six-way valve 300, and two ends of the dosing ring 600 are respectively connected with the port # 3 and the port # 6 of the first six-way valve 300; the # 1 port of the second six-way valve 400 is communicated with the # 6 port of the second six-way valve 400, the # 4 port of the second six-way valve 400 is communicated with the # 6 port of the second six-way valve 400, the # 5 port of the second six-way valve 400 is communicated with the # 4 port of the second six-way valve 400, and the # 3 port of the second six-way valve 400 is communicated with the # 2 port of the second six-way valve 400; the # 1 interface of the third six-way valve 700 is communicated with the # 6 interface of the third six-way valve 700, the # 6 interface of the third six-way valve 700 is communicated with the # 4 interface of the third six-way valve 700, the # 4 interface of the third six-way valve 700 is communicated with the # 5 interface of the third six-way valve 700, the # 3 interface of the third six-way valve 700 is communicated with the # 2 interface of the third six-way valve 700, the second pump body 200 transmits eluent to elute the second enrichment column 800 to realize online measurement, and meanwhile, the first pump body 100 transmits a mobile phase flushing quantification ring 600 to enable a first sample to be enriched to the first enrichment column 500.

It can be understood that, referring to fig. 8 or fig. 10, when the first six-way valve 300 is located at the first position, the # 6 port of the first six-way valve 300 is communicated with the # 1 port of the first six-way valve 300, the # 3 port of the first six-way valve 300 is communicated with the # 2 port of the first six-way valve 300, the quantitative ring 600 is disposed between the # 3 port of the first six-way valve 300 and the # 6 port of the first six-way valve 300, the # 5 port of the first six-way valve 300 is communicated with the first pump body 100, the # 4 port of the first six-way valve 300 is communicated with the second six-way valve 400, and the sample is injected into the quantitative ring 600, in this embodiment, a sample with a sample amount less than 15ml may be suitable;

referring to fig. 10, when the first six-way valve 300 is located at the first position, the second six-way valve 400 is located at the third position, and the third six-way valve 700 is located at the fifth position; a # 5 interface of the second six-way valve 400 is communicated with a # 4 interface of the first six-way valve 300, a # 6 interface of the second six-way valve 400 is communicated with a # 5 interface of the second six-way valve 400, a # 4 interface of the second six-way valve 400 is communicated with a # 6 interface of the second six-way valve 400, a # 3 interface of the second six-way valve 400 is communicated with a # 4 interface of the second six-way valve 400, a # 1 interface of the second six-way valve 400 is communicated with a # 2 interface of the second six-way valve 400, a # 1 interface of the second six-way valve 400 is connected with an outlet end of the first enrichment column 500, a # 2 interface of the second six-way valve 400 is connected with a detection device, and a # 3 interface of the second six-way valve 400 is connected with an inlet end of the second enrichment column 800; a # 1 port of the third six-way valve 700 is connected to an inlet end of the first enrichment column 500, a # 2 port of the third six-way valve 700 is communicated with a # 1 port of the third six-way valve 700, a # 2 port of the third six-way valve 700 is connected to the second pump body 200, a # 3 port of the third six-way valve 700 is connected to an outlet end of the second enrichment column 800, a # 4 port of the third six-way valve 700 is communicated with a # 3 port of the third six-way valve 700, a # 6 port of the third six-way valve 700 is communicated with a # 4 port of the third six-way valve 700, a # 5 port of the third six-way valve 700 is communicated with a # 6 port of the third six-way valve 700, and a # 5 port of the third six-way valve 700 is connected to the waste liquid. The second pump 200 delivers eluent to elute the first enrichment column 500 for on-line measurement, while a second sample is injected into the quantification loop 600.

Referring to fig. 9, 11, or 12, when the first six-way valve 300 is located at the second position, the # 6 port of the first six-way valve 300 is communicated with the # 5 port of the first six-way valve 300, the # 3 port of the first six-way valve 300 is communicated with the # 4 port of the first six-way valve 300, the # 1 port of the first six-way valve 300 is communicated with the # 2 port of the first six-way valve 300, the dosing ring 600 is disposed between the # 3 port of the first six-way valve 300 and the # 6 port of the first six-way valve 300, the # 5 port of the first six-way valve 300 is connected to the first pump body 100, and the # 4 port of the first six-way valve 300 is communicated with the second six-way valve 400 to deliver the mobile phase to flush the dosing ring 600.

Referring to fig. 9, with the first six-way valve 300 in the second position, the second six-way valve 400 is in the fourth position, and the third six-way valve 700 is in the sixth position; a # 5 interface of the second six-way valve 400 is communicated with a # 4 interface of the first six-way valve 300, a # 4 interface of the second six-way valve 400 is communicated with a # 5 interface of the second six-way valve 400, a # 6 interface of the second six-way valve 400 is communicated with a # 4 interface of the second six-way valve 400, a # 1 interface of the second six-way valve 400 is communicated with a # 6 interface of the second six-way valve 400, a # 2 interface of the second six-way valve 400 is communicated with a # 3 interface of the second six-way valve 400, the # 1 interface of the second six-way valve 400 is connected with an inlet end of the first enrichment column 500, the # 2 interface of the second six-way valve 400 is connected with a detection device, and the # 3 interface of the second six-way valve 400 is connected with an outlet end of the second enrichment column 800; the 1# interface of the third six-way valve 700 is connected with the outlet end of the first enrichment column 500, the 6# interface of the third six-way valve 700 is communicated with the 1# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is connected with the inlet end of the second enrichment column 800, and the 5# interface of the third six-way valve 700 is connected with waste liquid. The first pump 100 delivers a mobile phase flush dosing ring 600 to enrich the first sample to the first enrichment column 500.

Referring to fig. 11, when the first six-way valve 300 is located at the second position, the second six-way valve 400 is located at the third position, and the third six-way valve 700 is located at the fifth position; a # 5 interface of the second six-way valve 400 is communicated with a # 4 interface of the first six-way valve 300, a # 6 interface of the second six-way valve 400 is communicated with a # 5 interface of the second six-way valve 400, a # 4 interface of the second six-way valve 400 is communicated with a # 6 interface of the second six-way valve 400, a # 3 interface of the second six-way valve 400 is communicated with a # 4 of the second six-way valve 400, a # 1 interface of the second six-way valve 400 is communicated with a # 2 interface of the second six-way valve 400, a # 1 interface of the second six-way valve 400 is connected with an outlet end of the first enrichment column 500, a # 2 interface of the second six-way valve 400 is connected with a detection device, and a # 3 interface of the second six-way valve 400 is connected with an inlet end of the second enrichment column 800; a # 1 port of the third six-way valve 700 is connected to an inlet of the first enrichment column 500, a # 2 port of the third six-way valve 700 is communicated with a # 1 port of the third six-way valve 700, a # 2 port of the third six-way valve 700 is connected to the second pump body 200, a # 3 port of the third six-way valve 700 is connected to an outlet of the second enrichment column 800, a # 4 port of the third six-way valve 700 is communicated with a # 3 port of the third six-way valve 700, a # 6 port of the third six-way valve 700 is communicated with a # 4 port of the third six-way valve 700, a # 5 port of the third six-way valve 700 is communicated with a # 6 port of the third six-way valve 700, and a # 5 port of the third six-way valve 700 is connected to the waste liquid. The second pump 200 delivers eluent to elute the first enrichment column 500 for on-line measurement, while a second sample is injected into the quantification loop 600. The first pump 100 delivers a mobile phase flush dosing ring 600 to enrich the second sample to the second enrichment column 800.

Referring to fig. 12, with the first six-way valve 300 in the second position, the second six-way valve 400 is in the fourth position, and the third six-way valve 700 is in the sixth position; a # 5 interface of the second six-way valve 400 is communicated with a # 4 interface of the first six-way valve 300, a # 4 interface of the second six-way valve 400 is communicated with a # 5 interface of the second six-way valve 400, a # 6 interface of the second six-way valve 400 is communicated with a # 4 interface of the second six-way valve 400, a # 1 interface of the second six-way valve 400 is communicated with a # 6 interface of the second six-way valve 400, a # 2 interface of the second six-way valve 400 is communicated with a # 3 interface of the second six-way valve 400, the # 1 interface of the second six-way valve 400 is connected with an inlet end of the first enrichment column 500, the # 2 interface of the second six-way valve 400 is connected with a detection device, and the # 3 interface of the second six-way valve 400 is connected with an outlet end of the second enrichment column 800; the 1# interface of the third six-way valve 700 is connected with the outlet end of the first enrichment column 500, the 6# interface of the third six-way valve 700 is communicated with the 1# interface of the third six-way valve 700, the 4# interface of the third six-way valve 700 is communicated with the 6# interface of the third six-way valve 700, the 5# interface of the third six-way valve 700 is communicated with the 4# interface of the third six-way valve 700, the 2# interface of the third six-way valve 700 is connected with the second pump body 200, the 3# interface of the third six-way valve 700 is connected with the inlet end of the second enrichment column 800, and the 5# interface of the third six-way valve 700 is connected with waste liquid. The second pump block 200 delivers eluent to elute the second enrichment column 800 for on-line measurement, while the first pump block 100 delivers mobile phase wash dosing ring 600 to enrich the first sample to the first enrichment column 500.

The embodiment of the invention also provides an application method of the online enrichment treatment system, which is based on any one of the online enrichment treatment systems and comprises the following steps:

a first sample is injected into the quantitative loop 600;

starting the first pump 100 to deliver mobile phase flushing quantification ring 600 to enrich the first sample to the first enrichment column 500;

starting the second pump body 200 to deliver eluent to elute the first enrichment column 500, realizing on-line measurement, and simultaneously, feeding a second sample to the quantitative ring 600;

starting the first pump body 100 to convey the mobile phase to wash the quantitative ring 600, so that the second sample is enriched to the second enrichment column 800;

starting a second pump body 200 to deliver eluent to elute the second enrichment column 800, realizing on-line measurement, and simultaneously, feeding a first sample to a quantitative ring 600;

and circulating the steps.

The embodiment can realize accurate sample introduction through the quantitative ring, is also suitable for samples with the sample amount less than 15ml, and can realize the switching between the states of the six-way valve by arranging three six-way valves so as to enrich two samples on line.

Referring to fig. 13, in some embodiments of the present invention, the online enrichment processing system further comprises a programmable logic controller 11, and the state and position switching of each component in the online enrichment processing system are automatically controlled by the programmable logic controller 11.

The first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400, and the third six-way valve 700 are electrically connected to the programmable logic controller 11, respectively, the programmable logic controller 11 can control start and stop of the first pump body 100 and the second pump body 200, and can control switching of the first six-way valve 300 between the first position and the second position, the second six-way valve 400 between the third position and the fourth position, and the third six-way valve 700 between the fifth position and the sixth position, and under a preset program, a plurality of different control programs can be combined to meet detection requirements of the online enrichment processing system.

Referring to fig. 14, in particular, in some embodiments of the present invention, the programmable logic controller 11 includes a power module 111, a central processing unit 112, a storage module 113, an input module 114, an output module 115, and a peripheral interface module 116; the power module 111, the storage module 113, the input module 114, the output module 115 and the peripheral interface module 116 are electrically connected to the central processor 112, and the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400 and the third six-way valve 700 are electrically connected to the output module 115.

The peripheral interface module 116 may be connected to a computer or other device, and writes the online enrichment process program into the programmable logic controller 11, and stores the online enrichment process program written into the programmable logic controller 11 in the storage module 113, and when the online enrichment process program is activated, the central processor 112 invokes the online enrichment process program, and outputs a signal from the output module 115 to drive the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400, and the third six-way valve 700 to operate.

In some embodiments of the present invention, the output module 115 may be a pulse output unit. That is, the programmable logic controller 11 can drive the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400, and the third six-way valve 700 by using pulse signals.

In some embodiments of the present invention, the output module 115 may also be a digital output unit. That is, the programmable logic controller 11 can drive the first pump body 100, the second pump body 200, the first six-way valve 300, the second six-way valve 400, and the third six-way valve 700 by using digital signals.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. An online enrichment treatment system is characterized by comprising two pump bodies, a first six-way valve, a second six-way valve and an enrichment column;

the first and second six-way valves are in fluid communication, wherein one pump body is in fluid communication with the first six-way valve, wherein the other pump body is in fluid communication with the second six-way valve, and both the inlet and outlet ends of the enrichment column are in fluid communication with the second six-way valve;

when the first six-way valve is switched to a first state and the second six-way valve is correspondingly positioned at a first position, a sample is fed into the quantitative ring; when the first six-way valve is switched to a second state and the second six-way valve is correspondingly positioned at a second position, the pump body which is in fluid communication with the first six-way valve transmits a mobile phase to flush the quantitative ring, so that the sample is enriched to the enrichment column; and when the state of the first six-way valve is kept unchanged and the second six-way valve is correspondingly positioned at the first position, a pump body in fluid communication with the second six-way valve transmits eluent to elute the enrichment column for online measurement.

2. The on-line enrichment treatment system of claim 1, wherein the # 1 interface of the first six-way valve is connected with a sample injection device, and the # 2 interface is connected with the waste liquid;

3. The on-line enrichment processing system of claim 1, wherein the second six-way valve has a # 6 interface in communication with the first six-way valve, a # 5 interface connected to waste, and a # 2 interface connected to a detection device;

4. An application method of an on-line enrichment treatment system based on any one of claims 1 to 3, which is characterized by comprising the following steps:

feeding a sample to a quantitative ring through a first six-way valve;

starting a pump body in fluid communication with the first six-way valve to convey a mobile phase to flush the dosing ring and enrich the sample to the enrichment column;

and starting a pump body which is in fluid communication with the second six-way valve to convey eluent to elute the enrichment column, wherein the pump body is suitable for online measurement of samples with the sample volume of less than 15 ml.

5. An online enrichment treatment system is characterized by comprising two pump bodies, a first six-way valve, a second six-way valve, a third six-way valve, a first enrichment column and a second enrichment column;

6. The on-line enrichment processing system of claim 5, wherein a dosing ring is provided between the two ports of the first six-way valve;

when the first six-way valve is switched to a first state, the second six-way valve is correspondingly positioned at a first position, and the third six-way valve is correspondingly positioned at a third position, a pump body which is in fluid communication with the third six-way valve is used for conveying eluent to elute the first enrichment column for on-line determination, and meanwhile, a second sample is injected into a quantitative ring;

7. The on-line enrichment treatment system of claim 6, wherein the # 1 interface of the first six-way valve is connected with a sample injection device, and the # 2 interface is connected with the waste liquid;

8. The on-line enrichment processing system of claim 6, wherein the # 5 interface of the two-six way valve is in communication with the first six way valve, and the # 2 interface is connected with a detection device;

when the second six-way valve is located at the first position, the 6# interface of the second six-way valve is communicated with the 5# interface, the 4# interface is communicated with the 6# interface, the 3# interface is communicated with the 4# interface, the 2# interface is communicated with the 1# interface, the inlet end of the second enrichment column is communicated with the 3# interface of the second six-way valve, the outlet end of the second enrichment column is communicated with the third six-way valve, and the 1# interface is communicated with the outlet end of the first enrichment column;

9. The on-line enrichment treatment system of claim 6, wherein the # 2 port of the third six-way valve is in communication with one of the pump bodies, and the # 5 port is connected to waste liquid;

when the third six-way valve is located at a third position, the 1# interface of the third six-way valve is communicated with the 2# interface, the 3# interface is communicated with the 4# interface, the 4# interface is communicated with the 6# interface, the 6# interface is communicated with the 5# interface, the outlet end of the second enrichment column is communicated with the 3# interface, and the inlet end of the first enrichment column is communicated with the 1# interface;

10. An application method of an on-line enrichment treatment system based on any one of claims 5 to 9, which is characterized by comprising the following steps:

feeding a first sample to a quantitative ring through a first six-way valve;

and the steps are circulated.