CN115919704A - Saururus chinensis fermented raw stock as well as preparation method and application thereof - Google Patents
Saururus chinensis fermented raw stock as well as preparation method and application thereof Download PDFInfo
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- CN115919704A CN115919704A CN202211259033.9A CN202211259033A CN115919704A CN 115919704 A CN115919704 A CN 115919704A CN 202211259033 A CN202211259033 A CN 202211259033A CN 115919704 A CN115919704 A CN 115919704A
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Abstract
A saururus chinensis fermented raw stock and a preparation method and application thereof belong to the technical field of cosmetics. The invention obtains the saururus chinensis fermentation raw pulp by fermenting the raw material saururus chinensis through Bifidobacterium bifidum, and particularly obtains the saururus chinensis fermentation raw pulp by mixing saururus chinensis powder, a Bifidobacterium bifidum bacterial liquid and water to obtain an initial system, fermenting and culturing the initial system to obtain a fermentation liquid, and obtaining the saururus chinensis fermentation raw pulp by centrifugally filtering the fermentation liquid. The saururus chinensis fermented raw pulp obtained by the invention has the effects of resisting aging and whitening, and is safe to apply to cosmetics.
Description
Technical Field
The invention relates to saururus chinensis fermented raw pulp as well as a preparation method and application thereof, and belongs to the technical field of cosmetics.
Background
In recent years, there has been an increasing interest in "natural" ingredients in cosmetics, and not only is the effect of cosmetics sought, but also the safety of cosmetics is becoming a concern. Along with the needs of sellers, an increasing series of natural herbal skin care products are emerging in the cosmetics industry. The extraction of functional effective substances from natural plants is a hot spot of the research on the components of the current cosmetics. More and more products are also starting to pursue plant formulations. The saururus chinensis extract serving as a natural antioxidant, a whitening agent and a preservative has wide development and application prospects in the field of cosmetics.
(1) Saururus chinensis Baill extract has effects of scavenging free radicals, hydroxyl free radicals and superoxide free radicals, and the clearance rate increases with the increase of concentration. Therefore, the saururus chinensis extract has good capacity of removing free radicals and is a natural antioxidant with good activity.
(2) The saururus chinensis extract can inhibit proliferation of melanoma cells and activity of tyrosinase in the cells to different degrees, and can obviously reduce the amount of melanin in the cells. And the whitening performance of the saururus chinensis extract is superior to that of arbutin which is a common chemical whitening agent in cosmetics. Therefore, the saururus chinensis extract is a natural whitening additive with better performance.
(3) The saururus chinensis extract has good inhibition effect on escherichia coli and staphylococcus aureus. Can be used as a natural preservative to be applied to cosmetics.
(4) The saururus chinensis extract has very good light stability and heat stability. After the saururus chinensis extract is irradiated by ultraviolet light for different time and treated at different temperatures, the antioxidant activity of the saururus chinensis extract is basically kept unchanged.
The saururus chinensis baill extract serving as a natural antioxidant, a natural whitening agent and a natural preservative has a certain application prospect in the cosmetic industry. The Saururus chinensis Baill extract has high stability, can ensure long-term storage of the extract, and has little influence on the performance of the skin care product even if the Saururus chinensis Baill extract is added into the skin care product for heat treatment.
By adopting a microbial fermentation technology, the structure of active matters in plant raw materials can be modified and reformed by utilizing microbial cells or an enzyme catalytic reaction system in the cells, so that valuable new products are obtained. In addition, fermentation is also a process of converting carbohydrates and sugars into skin-preferred enzymes and amino acids for our skin through the metabolic activity of probiotics such as lactic acid bacteria. For example, the yeast fermentation product filtrate is not added with a drop of water, the components of the yeast fermentation product filtrate are close to skin cells, and sensitive muscles can also accept the yeast fermentation product filtrate. Therefore, the manufacturing process of the biological fermentation skin care product is different from the manufacturing process of the traditional skin care product which mixes active substances with other costs under the high temperature condition, the skin care product prepared by fermentation needs to be subjected to a slow fermentation process in a specific environment, the content of the obtained active substances is higher, and the regulating effect on the skin micro-ecology of people is better.
Disclosure of Invention
The invention aims at the problems and provides the saururus chinensis fermented raw pulp and the preparation method and the application thereof. The technical scheme of the invention is as follows:
a preparation method of saururus chinensis fermented raw pulp comprises the following steps:
mixing Saururi herba powder, bifidobacterium bifidum liquid and water to obtain initial system, performing fermentation culture on the initial system to obtain fermentation broth, and centrifuging and filtering the fermentation broth to obtain Saururi herba fermented raw stock. The saururus chinensis powder and the water mixed solution are used as a control, and other variables are kept consistent. The data show that bifidobacterium bifidum can biologically synthesize micromolecules such as amino acid, organic acid, flavone, polyphenol and the like which are easy to absorb, and can also produce macromolecules such as polysaccharide, polypeptide or protein and the like with important effects by fermenting the saururus chinensis.
Further, the concentration of the bifidobacterium bifidum bacterial liquid is 10 6 ～10 8 cfu/ml, pH 6.5-7.5.
Further, the mass volume ratio of the saururus chinensis powder to the bifidobacterium bifidum liquid to the water is 10-80; preferably, the ratio of 20:10:150,g/ml/ml.
Further, the particle size of the saururus chinensis powder is 80-200 meshes; preferably 100 mesh.
Further, the conditions of fermentation culture are as follows: fermenting in a constant temperature incubator at 37 ℃ for 40 hours;
the invention also comprises the steps of carrying out centrifugal filtration on the fermentation liquor, carrying out high-pressure sterilization on the filtrate for 30min at the temperature of 110 ℃ to inactivate strains, centrifuging the sterilized fermentation filtrate for 30min under the conditions of 12000r/min and the centrifugal radius of 9cm, removing the precipitate, and collecting the supernatant, namely the saururus chinensis benth fermentation raw stock.
The invention also comprises the saururus chinensis fermented raw pulp prepared by the preparation method and application of the saururus chinensis fermented raw pulp in preparing cosmetics; preferably, the cosmetic is any one selected from a lotion, an emulsion, a cream, a mask, a cleanser, or a BB cream.
Furthermore, the addition amount of the saururus chinensis fermented raw pulp in the cosmetic is 5 percent by mass. Compared with the prior art, the invention has the following advantages:
(1) The preparation process provided by the invention has high extraction rate, and the fermentation extraction method has the advantages that the production process can be effectively simplified, so that the reaction process is carried out under mild conditions. Meanwhile, the fermentation extraction method can also change the molecular structure of the components of the raw materials, improve the efficacy of the raw materials, reduce toxicity and irritation, optimize the color, taste and other advantages, is an ideal new process for extracting the traditional Chinese medicine, and is beneficial to popularization and application.
(2) The fermented raw juice of the traditional Chinese medicine composition can effectively brighten skin color when being used in cosmetics, has obvious and effective antioxidant effect, has good whitening effect and has huge market value.
Drawings
FIG. 1 is a graph showing the effect of different dilution factors of saururus chinensis fermented puree on DPPH free radical scavenging ability;
FIG. 2 is a graph comparing the removal rates of DPPH free radicals by VE, astaxanthin, coenzyme Q10, VC, fullerene, carnosine, ergothioneine and the P.lizardtail fermentation puree of the present invention;
FIG. 3 is a graph showing the effect of different dilution times of saururus chinensis fermented raw stock on tyrosinase inhibition ability according to the present invention;
FIG. 4 is a comparison graph of the inhibition effect of arbutin with a mass fraction of 1%, alpha arbutin with a mass fraction of 3%, nicotinamide with a mass fraction of 1%, nicotinamide with a mass fraction of 2%, nicotinamide with a mass fraction of 5%, saururi herba extract, motherwort extract, sparrow extract, and sapphire blackening extract with a mass fraction of 1.5% on tyrosinase; the Saururi herba extract, herba Leonuri extract, and flos Caraganae Sinicae extract are obtained from Guangzhou Zhenghe Co., ltd, and have models of L1-SCB1503, L2-SCB2465, and L1-SCB1674.
Detailed Description
The invention is further described below in conjunction with specific embodiments, and the advantages and features of the invention will become more apparent as the description proceeds. The examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Example 1: saururus chinensis fermented raw stock and preparation method thereof
The method comprises the following steps:
mixing Saururi herba powder, bifidobacterium bifidum liquid and water to obtain an initial system, fermenting the initial system in a constant temperature incubator at 37 ℃ for 40 hours to obtain a fermentation liquid, performing centrifugal filtration on the fermentation liquid, performing high pressure sterilization on the filtrate at 110 ℃ for 30min to inactivate strains, centrifuging the sterilized fermentation filtrate for 30min under the conditions of 12000r/min and a centrifugal radius of 9cm, discarding the precipitate, and collecting the supernatant to obtain Saururi herba fermentation raw pulp;
the concentration of the bifidobacterium bifidum liquid is 10 7 cfu/ml, pH 7; the mass volume ratio of the saururus chinensis powder to the bifidobacterium bifidum liquid to the water is 20:10:150,g/ml/ml; the particle size of the saururus chinensis powder is 100 meshes.
Example 2: saururus chinensis fermented raw stock and preparation method thereof
The concentration of the bifidobacterium bifidum liquid is 10 8 cfu/ml, pH 6.5; the mass volume ratio of the saururus chinensis powder to the bifidobacterium bifidum liquid to the water is 50:20:350,g/ml/ml; the saururus chinensis powder has a particle size of 100 meshes, and the method is otherwise the same as that of example 1.
Example 3: saururus chinensis fermented raw stock and preparation method thereof
The concentration of the bifidobacterium bifidum liquid is 10 6 cfu/ml, pH 7.5; the mass-volume ratio of the saururus chinensis powder to the bifidobacterium bifidum liquid to the water is 80:30:550,g/ml/ml; the saururus chinensis powder has a particle size of 100 meshes, and the method is otherwise the same as that of example 1.
Experimental example 1 a mixture of saururus chinensis powder and water and a fermented puree obtained according to the present invention were tested for DPPH radical scavenging and tyrosinase inhibition, wherein the proportions of saururus chinensis powder and water were the same as those of the present invention. The results are shown in table 1:
table 1 test example 1 test results
| DPPH radical clearance | Tyrosinase inhibition rate | |
| Saururi herba powder and water mixed solution | 57.38％ | 45.88％ |
| The invention ferments the original pulp | 71.17％ | 65.10％ |
As can be seen from Table 1, the clearance of free radicals was greatly improved after fermentation of Saururi herba with Bifidobacterium, and compared with the positive control, the clearance was 0.03mg/ml V E Has the same antioxidant effect. It can be seen that the bifidobacterium bifidum saururus chinensis fermented raw pulp greatly improves the antioxidant effect of saururus chinensis.
The saururus chinensis has low tyrosinase inhibition capability, and after the fermentation of bifidobacterium bifidum, the tyrosinase inhibition capability is 1.5 times that of the saururus chinensis. The tyrosinase inhibition ability obtained after fermentation is equivalent to the tyrosinase inhibition rate of 0.1mg/ml kojic acid. Therefore, the bifidobacterium bifidum and saururus chinensis fermented raw pulp is greatly improved in whitening and antioxidant effects.
Experimental method for scavenging free radicals (DPPH)
The positive control is dissolved and diluted by 95% ethanol to form: the test system was verified by using a series of concentration gradients of 0.08mg/mL, 0.04mg/mL, 0.02mg/mL, and 0.01 mg/mL.
Treating a test object: the water-soluble test substance is diluted into a multi-concentration sample by water, and the oil-soluble test substance is diluted into a multi-concentration sample by 95% ethanol.
Referring to Table 2, a 10mL test tube was used to set up the sample tube (T), sample background (T) 0 ) DPPH tube (C) and solvent background (C) 0 ) For each sample concentration to be tested, 3 parallel tubes are required for each sample tube (T), and 3 parallel tubes are required for DPPH tube (C).
In the sample tube (T) and sample background (T) 0 ) 1mL of the same concentration of each sample solution was added.
In all test tubes (T, T) 0 And C, C) supplementing a solvent, using water for a water-soluble sample, using 95% ethanol for an oil-soluble sample to supplement 3mL, and uniformly mixing.
1mL of DPPH ethanol solution was added to the sample tubes (T) and DPPH tube (C), the sample background (T) 0 ) And solvent background (C) 0 ) Replacing with 95% ethanol, shaking, and standing at room temperatureFor 5 minutes. Each reaction solution was transferred to a 1cm cuvette and absorbance was measured at 517 nm.
TABLE 2DPPH test sample liquid addition requirements
| T-sample tube | T 0 Background of sample | C-DPPH tube | C 0 Background of solvent | |
| Sample solution (mL) | 1 | 1 | — | — |
| Water or 95% ethanol solvent (mL) | 2 | 2 | 3 | 3 |
| DPPH ethanol solution (mL) | 1 | — | 1 | — |
| 95% ethanol (mL) | — | 1 | — | 1 |
| Number of parallels | 3/sample | 1/sample | 3/test | 1/test |
Calculating DPPH free radical clearance rate:
tyrosinase activity inhibition experimental method
The positive control was diluted with disodium hydrogen phosphate-citric acid buffer ph6.8 to: a series of concentration gradients of 1mg/mL, 0.2mg/mL, 0.04mg/mL, 0.008mg/mL were used to validate the assay system.
Treating a test object: the test substance is diluted into a multi-concentration sample by using a disodium hydrogen phosphate-citric acid buffer solution.
Referring to Table 3, a 10mL test tube was used to set up the sample tube (T), sample background (T) 0 ) Enzyme reaction tube (C) and solvent background (C) 0 ) For each sample, 3 parallel tubes are required to be set for each sample tube (T) of each concentration to be tested, and 3 parallel tubes are required to be set for the enzyme reaction tube (C).
In the sample tube (T) and sample background (T) 0 ) 1mL of the same concentration of each sample solution, enzyme reaction tube (C) and solvent background (C) 0 ) 1mL of disodium hydrogen phosphate-citric acid buffer was added.
Adding 0.5mL of tyrosinase solution into each of the sample tube (T) and the enzyme reaction tube (C), and obtaining a sample background (T) 0 ) With background of solvent (C) 0 ) With 0.5mL disodium hydrogen phosphate-citric acid bufferInstead, the sample and tyrosinase were mixed well and incubated in a 37 ℃ water bath for 10 minutes.
And sequentially adding 2mL of levodopa solution into each tube, controlling the reaction time of each tube to be 5 minutes, immediately transferring each tube of reaction solution into a cuvette, and measuring the light absorption value at 475 nm.
TABLE 3 tyrosinase inhibition test sample addition requirements
Calculation of tyrosinase inhibition:
test example 2: the influence of different dilution times of the saururus chinensis fermented raw pulp on the DPPH free radical scavenging capacity
As shown in figure 1, the DPPH free radical clearance rate is more than 50% when the fermentation broth is diluted by 2 times, 4 times and 8 times, namely, the DPPH free radical clearance rate exceeds half of the inhibiting concentration, and the antioxidant effect of the fermentation broth is still equivalent to that of 0.02mg/ml VE when the fermentation broth is diluted by 16 times, so that the antioxidant effect of the fermentation broth of the saururus chinensis.
Test example 3: VE, astaxanthin, coenzyme Q10, VC, fullerene, carnosine and ergothioneine compared with the saururus chinensis fermented raw pulp of the invention on the DPPH free radical clearance
As shown in figure 2, the difference of DPPH free radical scavenging rate between the saururus chinensis fermentation raw stock and VE, VC and ergothioneine is not significant and reaches more than 70 percent. The common antioxidants astaxanthin, coenzyme Q10, fullerene and carnosine are far lower than the saururus chinensis fermentation raw stock, which shows that the saururus chinensis fermentation raw stock has good oxidation resistance.
Test example 4: the inhibition capability of the Chinese lizardtail herb fermented raw pulp on tyrosinase by different dilution times
As shown in figure 3, the feed has a high tyrosinase inhibition rate of 65.10% when the feed is not diluted. The tyrosinase inhibition rate is gradually reduced along with the increase of the dilution concentration, and when the dilution concentration is more than 8 times, the change of the tyrosinase inhibition rate is reduced slightly. Therefore, in practical applications, the smaller the dilution factor, the better the whitening effect.
Test example 5: the method is used for detecting the tyrosinase inhibition with 1% of arbutin, 3% of alpha arbutin, 1% of nicotinamide, 2% of nicotinamide, 5% of nicotinamide, a Saururi herba extracting solution, a motherwort extracting solution, a sparrow extracting solution and 1.5% of sapphire after black fading and refining; the Saururi herba extract, herba Leonuri extract, and flos Caraganae Sinicae extract are obtained from Guangzhou Zhenghe Co., ltd, and have models of L1-SCB1503, L2-SCB2465, and L1-SCB1674.
As shown in fig. 4, the inhibition ability of the saururus chinensis fermented raw pulp obtained by the invention on tyrosinase is far higher than that of arbutin, 1% nicotinamide, 2% nicotinamide, a saururus chinensis extracting solution on the market, a motherwort extracting solution, a sparrow extracting solution and 1.5% sapphire black fading and refining, and the difference is very obvious; the cosmetic effect is equivalent to that of a cosmetic with 5% of the addition amount of nicotinamide, so that the saururus chinensis fermented raw stock disclosed by the invention has a very good whitening effect.
Application example 1: whitening, brightening and skin softening emulsion added with Chinese herbal medicine fermented primary pulp of saururus chinensis
The preparation method comprises the following steps:
(1) Uniformly mixing the group A in advance for later use;
(2) Scattering the group B powder into water, stirring uniformly, heating to 80-82 ℃, dissolving completely and uniformly, homogenizing for 1-2 minutes, and defoaming for later use;
(3) Dissolving group C and group D in advance for later use;
(4) Cooling group B to below 40 deg.C, sequentially adding groups C, D and E, and stirring;
(5) Slowly adding group A into (4).
Group A
Group B
Group C
AM 91 0.4 parts
0.4 part of 1, 2-hexanediol
Group D
Promosorb G (plant penetration enhancer) 3 parts
10 portions of saururus chinensis fermented raw stock
Application example 2: whitening face cream added with saururus chinensis fermented raw juice
The preparation method comprises the following steps:
(1) Mixing group A, heating to 80-85 deg.C, stirring until the solid is completely melted into liquid, and keeping the temperature for use;
(2) Mixing and heating the group B to 80-85 ℃, stirring until the solid is completely melted into liquid, and keeping the temperature for later use;
(3) Slowly adding the group A into the group B under weak and medium speed stirring, and homogenizing at medium speed for 1-3 minutes;
(4) Adding the group C and the group D into the step (3), and stirring at a low speed for 5 minutes to obtain the compound;
group A
Group B
Group C
AM 91 0.4 part
0.4 part of 1, 2-hexanediol
Group D
7.6 parts of three-hundred-herb fermented raw stock
Test example 6: human body patch test
Test method
The selected area is 40mm 2 And qualified spot test equipment with the depth of about 1 mm. And (3) test treatment: 0.025mL of moisturizing cosmetic was placed in a chamber of a patch tester, the patch tester with the test substance added thereto was applied to the curved side of the forearm of the subject with hypoallergenic tape, and gently pressed with the palm to be uniformly applied to the skin for 24 hours. Blank control treatment is set for each test, no substance is placed in the control spot tester hole, and other processes are the same as those of the test group.
The skin reactions were observed according to the standard of table 1 30min (after disappearance of the indentation), 24h and 48h after removal of the test article plaque test device, respectively, and the observation results were recorded.
Skin closed patch test skin reaction degree grading criteria are as follows:
"-" = negative reaction;
"±" = suspicious reaction, only weak erythema;
"+" = weak positive reaction (erythema reaction); erythema, infiltration, edema, and possibly pimples;
"++" = strong positive reaction (herpetic response); erythema, infiltration, edema, pimples, herpes; the reaction may be beyond the test area;
"+ + + +" = very strong positive reaction (fusogenic herpes response); obvious erythema, severe infiltration, edema, and fusional herpes; the reaction goes beyond the test area.
The judgment standard of the human body patch test is as follows: the number of plus or minus adverse reactions in 30 subjects is more than 5, or the number of plus or minus adverse reactions is more than 2, or any skin adverse reaction above 1 plus or minus plus is judged to have adverse reactions on human body, otherwise, the human body is judged to have no adverse reactions.
As can be seen from table 4: the test results obtained in the application examples 1 and 2 do not produce adverse reactions, which shows that the saururus chinensis fermented raw pulp provided by the invention has safety and does not bring adverse reactions to human bodies.
Table 4 test results of the patch test for forearm obtained in application example 1 and application example 2
Claims (10)
1. A preparation method of saururus chinensis fermented raw pulp is characterized by comprising the following steps:
bifidobacterium bifidum is fermented by taking Saururi herba as a raw material to obtain Saururi herba fermented raw pulp.
2. The method of claim 1, comprising the steps of:
mixing Saururi herba powder, bifidobacterium bifidum liquid and water to obtain initial system, performing fermentation culture on the initial system to obtain fermentation broth, and centrifuging and filtering the fermentation broth to obtain Saururi herba fermented raw stock.
3. The method according to claim 1, wherein the concentration of the Bifidobacterium bifidum bacterial liquid is 10 6 ～10 8 cfu/ml, pH 6.5-7.5.
4. The preparation method according to claim 2, wherein the mass-volume ratio of the saururus chinensis powder to the bifidobacterium bifidum liquid to the water is 10-80; the particle size of the saururus chinensis powder is 80-200 meshes.
5. The method according to claim 3, wherein the mass-to-volume ratio of the Saururus chinensis powder, the Bifidobacterium bifidum solution, and water is 20:10:150,g/ml/ml; the particle size of the saururus chinensis powder is 100 meshes.
6. The method according to claim 2, wherein the conditions of the fermentation culture are: fermenting in a constant temperature incubator at 37 ℃ for 40 hours.
7. The preparation method of claim 2, wherein the fermentation broth is centrifuged and filtered, the filtrate is autoclaved at 110 ℃ for 30min to inactivate the strains, the sterilized fermentation filtrate is centrifuged at 12000r/min with a centrifugation radius of 9cm for 30min, the precipitate is discarded, and the supernatant is collected to obtain the saururus chinensis fermented puree.
8. Saururus chinensis fermented puree obtained by the preparation method according to any one of claims 1 to 7.
9. The use of the saururus chinensis fermented puree of claim 8 in the preparation of cosmetics.
10. The use according to claim 9, wherein the cosmetic is selected from any one of a lotion, an emulsion, a cream, a mask, a face cleanser or a BB cream; preferably, the addition amount of the saururus chinensis fermented raw pulp in the cosmetic is 5% by mass.
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