CN115381749A

CN115381749A - Motherwort fermented raw stock and preparation method and application thereof

Info

Publication number: CN115381749A
Application number: CN202211164445.4A
Authority: CN
Inventors: 刘青; 周娟; 闫利娟
Original assignee: Calais Jinan Life Technology Co ltd
Current assignee: Calais Jinan Life Technology Co ltd
Priority date: 2022-09-23
Filing date: 2022-09-23
Publication date: 2022-11-25

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to motherwort fermented protoplasm as well as a preparation method and application thereof. The motherwort herb fermented primary pulp is obtained by mixing motherwort herb powder, bacterial liquid and water to obtain an initial system, fermenting and culturing the initial system to obtain fermentation liquor, sterilizing and centrifuging the fermentation liquor to obtain supernatant. The preparation process provided by the invention has high extraction rate, and the fermentation extraction method has the advantages that the production process can be effectively simplified, so that the reaction process is carried out under mild conditions. Meanwhile, the fermentation extraction method can also change the molecular structure of the components of the raw materials, improve the efficacy of the raw materials, reduce toxicity and irritation, optimize the color, taste and other advantages, is an ideal new process for extracting the traditional Chinese medicine, and is beneficial to popularization and application. When the motherwort herb fermented raw stock is used in cosmetics, the skin color can be effectively brightened, the obvious effective antioxidant effect is achieved, the whitening effect is good, and the market value is huge.

Description

Motherwort fermented raw stock and preparation method and application thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to motherwort fermented raw pulp as well as a preparation method and application thereof.

Background

In recent years, with the rapid development of the cosmetic industry and the wide application of the green concept in various industries, plant extracts have the obvious advantages of natural safety and small toxic and side effects, so that the plant extracts are widely applied to anti-aging whitening cosmetics as active raw materials. Along with consumer demand, the cosmetic industry is emerging with an increasing array of natural herbal skin care. The extraction of functional effective substances from natural plants is a hot spot of the research on the components of the current cosmetics. The Chinese herbal medicine serving as a natural antioxidant, a whitening agent and a preservative has wide development and application prospects in the field of cosmetics.

Motherwort is a cosmetic product handed down from ancient palace, a formula for beautifying motherwort after Tianda Shenghuang with similar effect is recorded in Tang Dynasty's medical classics of exterior station secretary, and according to the statement in the formula, motherwort ash not only has the effect of whitening, but also can eliminate wrinkles of the skin of old people, so that the motherwort has the magical effect of returning old people to young people for more than middle-aged women. In addition, it can also erode the old, rough stratum corneum layer of the skin, allowing the dead skin to slough off. Motherwort, which is a very popular beauty herb for women, is a beauty product for ancient people. Modern pharmacopoeia also determines that motherwort contains various trace elements and has the effects of oxidation resistance, aging resistance and even cancer resistance.

Motherwort has the radiation protection function, and the motherwort can reduce apoptosis by regulating the expression of Bax/Bcl-2 protein. Rutin in herba Leonuri has strong absorption effect on ultraviolet rays and X rays, and is added into sunscreen agent in an amount of 10% to achieve ultraviolet absorption rate of 98%. Herba Leonuri can improve survival rate of keratinocytes after UVB irradiation. Therefore, the cosmetic prepared from the traditional Chinese medicine motherwort has good sun-proof and ultraviolet-proof effects.

Herba Leonuri has free radical scavenging effect, and herba Leonuri polysaccharide has effects of scavenging hydroxy free radical and superoxide radical, and protecting DNA from oxidation damage, and has positive correlation with polysaccharide concentration. Researches find that motherwort contains abundant flavones, and the total flavones extracted from motherwort have good scavenging effect on hydroxyl radicals.

The motherwort has the functions of nourishing and moistening skin, contains components such as protein, various amino acids, lipids, vitamins and the like, is used for cosmetics, can nourish skin and hair, can enhance the oxidation resistance of organisms, improve skin microcirculation, increase the content of collagen fibers and collagen protein of the skin and the like. And has moisture keeping effect on skin, and makes the cosmetic cream soft and comfortable.

The motherwort has the whitening effect, and researches show that the fresh motherwort juice can increase the content of hydroxyproline and fibroblasts in aged skin, and can also inhibit the activity of tyrosinase and the proliferation of B-16 melanoma cells, thereby playing the roles of eliminating blackness and facial spots and beautifying. Leonurine, stachydrine, lauric acid, oleic acid and other substances contained in the motherwort can promote the metabolism of the skin, fully nourish the skin, and make the skin white and moist, thereby playing a good role in nutrition and health care on the skin.

Motherwort has the functions of diminishing inflammation and inhibiting bacteria, and the anti-inflammatory function of motherwort may be related to the improvement of local blood circulation, the reduction of exudation and the acceleration of absorption. The alkaloid in herba Leonuri has good effects of killing and inhibiting various bacteria and fungi.

By adopting a microbial fermentation technology, the structure of active matters in plant raw materials can be modified and reformed by utilizing microbial cells or an enzyme catalytic reaction system in the cells, so that valuable new products are obtained. In addition, fermentation is also a process of converting carbohydrates and sugars into skin-preferred enzymes and amino acids for our skin through the metabolic activity of probiotics such as lactic acid bacteria. For example, the yeast fermentation product filtrate is not added with a drop of water, the components of the yeast fermentation product filtrate are close to skin cells, and sensitive muscles can also accept the yeast fermentation product filtrate. Thus, unlike the manufacturing process of conventional skin care products in which active substances and other costs are mixed at high temperatures, bio-fermented skin care products are produced which undergo a slow fermentation process in a clean, oxygen-free environment for several weeks. The obtained active substance has higher content and better regulating effect on the skin micro-ecology.

The invention screens natural traditional Chinese medicine components with the effects of resisting oxidation and whitening, provides a safe and effective cosmetic from the perspective of fermenting, resisting oxidation and whitening Chinese herbal medicines, and has development prospect and economic value.

Disclosure of Invention

Aiming at the vacancy of the existing market and the technical defects, the invention provides the motherwort herb fermented raw stock with the effects of resisting oxidation and whitening, and the preparation method and the application thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

a herba Leonuri fermented protoplasm: mixing motherwort powder, bacterial liquid and water to obtain an initial system, and performing fermentation culture on the initial system to obtain motherwort fermentation raw stock;

the initial system comprises the following motherwort in parts by mass: 10-80 parts of motherwort;

the effective component is supernatant fluid obtained by sterilizing and centrifuging fermentation liquor obtained by fermenting an initial system obtained by mixing traditional Chinese medicines, water and bacterial liquid.

The bifidobacterium bifidum is used for fermenting the traditional Chinese medicine composition, so that the efficacy and activity of the traditional Chinese medicine composition are retained, the loss of active ingredients caused by the traditional extraction mode is avoided, the preparation process is simple, the condition is mild, the industrial large-scale production can be realized, good economic benefits can be brought to enterprises, and the bifidobacterium bifidum has a wide prospect. The invention also discloses a preparation method of the motherwort herb fermented primary pulp, which comprises the following steps:

(1) Preparing materials: removing impurities from herba Leonuri, cleaning, oven drying, and pulverizing to 100 mesh;

(2) Strain culture

Activating strains: selecting a bacterial colony I, placing the bacterial colony I in a liquid culture medium, and activating the strain in a shaking table to obtain activated bacterial liquid;

and (3) strain purification: diluting and plating the activated bacteria liquid in a gradient manner so as to obtain a single bacterial colony and obtain a purified strain;

and (3) strain amplification culture: inoculating the purified strain to BS culture medium (pH 7.0), standing in 37 deg.C incubator, and obtaining zymocyte liquid when OD =0.6-1.0, the strain is in log phase and has concentration of (10) ⁶ -10 ⁸ CFU/mL)；

(3) Fermentation of

The preparation concentration is 10 ⁶ ～10 ⁸ cfu/mL zymocyte liquid, and adjusting the pH value to 6.5-7.5;

mixing the zymophyte liquid, the motherwort powder and water according to the proportion, and fermenting for 40 hours in a constant-temperature incubator at 37 ℃ to obtain fermentation liquid;

(4) Purification of

Filtering the obtained fermentation liquor, sterilizing the filtrate for 30min under high pressure at the temperature of 121 ℃ to inactivate strains, centrifuging the sterilized fermentation filtrate for 30min under the conditions of 12000r/min and a centrifugation radius of 9cm, removing the precipitate, and collecting the supernatant, namely the motherwort herb fermentation protoplasm provided by the invention.

The dosage ratio of zymophyte liquid, motherwort and water in the motherwort primary fermentation pulp is 0-30 mL: 10-80 g: 50-600 g.

In the bacterial liquid, the strain is Bifidobacterium bifidum (Bifidobacterium bifidum).

The motherwort herb is prepared from dry powder of the motherwort herb, and the particle size of the motherwort herb is 80-200 meshes, and particularly 100 meshes.

The invention also discloses application of the motherwort herb fermented raw pulp in cosmetics. The cosmetic contains the motherwort fermented raw stock, and the dosage of the motherwort fermented raw stock is 5%.

Preferably, the cosmetic comprises a lotion, an emulsion, a cream, a mask, a cleanser or a BB cream.

Advantageous effects

The invention discloses motherwort fermented protoplasm and a preparation method and application thereof, and compared with the prior art, the motherwort fermented protoplasm has the following advantages:

(1) The preparation process provided by the invention has high extraction rate, and the fermentation extraction method has the advantages that the production process can be effectively simplified, and the reaction process is carried out under mild conditions. Meanwhile, the fermentation extraction method can also change the molecular structure of the components of the raw materials, improve the efficacy of the raw materials, reduce toxicity and irritation, optimize the color, taste and other advantages, is an ideal new process for extracting the traditional Chinese medicine, and is beneficial to popularization and application.

(2) When the motherwort herb fermented raw stock is used in cosmetics, the skin color can be effectively brightened, the obvious effective antioxidant effect is achieved, the whitening effect is good, and the market value is huge.

Drawings

FIG. 1: a graph of the effect of different dilution times of bifidobacterium bifidum and motherwort herb fermentation raw stock on DPPH free radical scavenging capacity;

FIG. 2: the DPPH free radical clearance rate comparison graph of the motherwort fermentation raw stock and the market raw material is shown in the invention;

FIG. 3: a graph of the effect of different dilution times of bifidobacterium bifidum and motherwort herb fermentation raw stock on tyrosinase inhibition capability;

FIG. 4 is a schematic view of: the invention relates to a comparison graph of the inhibition rate of fermented motherwort herb puree and tyrosinase which is a commercially available whitening raw material.

Detailed Description

Hereinafter, the present invention will be described in detail. Before the description is made, it should be understood that the terms used in the present specification and the appended claims should not be construed as limited to general and dictionary meanings, but interpreted based on the meanings and concepts corresponding to technical aspects of the present invention on the basis of the principle that the inventor is allowed to define terms appropriately for the best explanation. Accordingly, the description proposed herein is just a preferable example for the purpose of illustrations only, not intended to limit the scope of the invention, so it should be understood that other equivalents and modifications could be made thereto without departing from the spirit and scope of the invention.

The following examples are given by way of illustration of embodiments of the invention and are not to be construed as limiting the invention, and it will be understood by those skilled in the art that modifications may be made without departing from the spirit and scope of the invention. Unless otherwise specified, reagents and equipment used in the following examples are commercially available products.

Example 1

A preparation method of motherwort fermented protoplasm comprises the following steps:

(2) Culture of bacterial species

Activating strains: selecting a colony, namely, putting the colony in a liquid culture medium, and activating a strain in a shaking table to obtain activated bacterial liquid;

(3) Fermentation of

The preparation concentration is 10 ⁶ ～10 ⁸ cfu/mL zymocyte liquid, and adjusting the pH value to 6.5;

(4) Purification of

The dosage ratio of zymophyte liquid, motherwort and water in the motherwort fermentation protoplasm is 30mL:80g:600g.

Example 2

(2) Strain culture

and (3) strain amplification culture: inoculating the purified strain to BS culture medium (pH 7.0), standing in 37 deg.C incubator, and obtaining zymocyte liquid when OD =0.6-1.0, the strain is in log phase and has concentration of 10 ⁶ -10 ⁸ CFU/mL)；

(3) Fermentation of

The preparation concentration is 10 ⁶ ～10 ⁸ Adjusting the pH value of the cfu/mL zymocyte liquid to 7.5;

(4) Purification of

The dosage ratio of zymophyte liquid, motherwort and water in the motherwort fermentation protoplasm is 20mL:50g:200g.

Example 3

(2) Culture of bacterial species

(3) Fermentation of

The preparation concentration is 10 ⁶ ～10 ⁸ cfu/mL zymophyte liquid, and adjusting the pH value to 7;

(4) Purification of

Filtering the obtained fermentation liquor, sterilizing the filtrate at 121 ℃ for 30min under high pressure to inactivate strains, centrifuging the sterilized fermentation filtrate for 30min under the conditions of 12000r/min and the centrifugal radius of 9cm, removing the precipitate, and collecting the supernatant, namely the motherwort herb fermentation raw stock.

The dosage ratio of zymophyte liquid, motherwort and water in the motherwort fermentation protoplasm is 10mL:10g:50g.

Experimental example 1 Effect of Bifidobacterium bifidum fermented motherwort

1. Experimental method for scavenging free radicals (DPPH)

The positive control is dissolved and diluted by 95% ethanol to form: the test system was verified by using a series of concentration gradients of 0.08mg/mL, 0.04mg/mL, 0.02mg/mL, and 0.01 mg/mL.

Treating a test object: the water-soluble test substance is diluted with water to obtain a multi-concentration sample, and the oil-soluble test substance is diluted with 95% ethanol to obtain a multi-concentration sample.

Referring to Table 2, a 10mL test tube was used to set up the sample tube (T), sample background (T) ₀ ) DPPH tube (C) and solvent bookBottom (C) ₀ ) For each sample concentration to be tested, 3 parallel tubes are required for each sample tube (T), and 3 parallel tubes are required for DPPH tube (C).

In the sample tube (T) and sample background (T) ₀ ) 1mL of the same concentration of sample solution was added to each of them.

In all test tubes (T, T) ₀ And C, C) supplementing a solvent, using water for a water-soluble sample, using 95% ethanol for an oil-soluble sample to supplement 3mL, and uniformly mixing.

1mL of DPPH ethanol solution was added to the sample tubes (T) and DPPH tube (C), the sample background (T) ₀ ) And solvent background (C) ₀ ) Replace with 95% ethanol, shake gently, and stand at room temperature for 5 minutes.

Each reaction solution was transferred to a 1cm cuvette and absorbance was measured at 517 nm.

TABLE 2 sample addition requirements

Calculating DPPH free radical clearance rate:

2. tyrosinase activity inhibition experimental method

The positive control was diluted with disodium phosphate-citric acid buffer ph6.8 to: a series of concentration gradients of 1mg/mL, 0.2mg/mL, 0.04mg/mL, 0.008mg/mL were used to validate the assay system.

Treating a test object: the test substance is diluted into a multi-concentration sample by using a disodium hydrogen phosphate-citric acid buffer solution.

Referring to Table 2, a 10mL test tube was used to set up the sample tube (T), sample background (T) ₀ ) Enzyme reaction tube (C) and solvent background (C) ₀ ) 3 parallel tubes are required to be set up for each sample tube (T) of each concentration to be tested for each sample, and 3 parallel tubes are required to be set up for the enzyme reaction tube (C).

In the sample tube (T) and sample background (T) ₀ ) In each case 1mL of phase was addedSame concentration of sample solution, enzyme reaction tube (C) and solvent background (C) ₀ ) Then 1mL of disodium hydrogen phosphate-citric acid buffer was added.

Adding 0.5mL of tyrosinase solution into each of the sample tube (T) and the enzyme reaction tube (C), and obtaining a sample background (T) ₀ ) With background of solvent (C) ₀ ) Replace with 0.5mL disodium hydrogen phosphate-citric acid buffer, mix the sample and tyrosinase well, put in 37 ℃ water bath and incubate for 10 minutes.

And sequentially adding 2mL of levodopa solution into each tube, controlling the reaction time of each tube to be 5 minutes, immediately transferring each tube of reaction solution into a cuvette, and measuring the light absorption value at 475 nm.

TABLE 2 sample addition requirements

Calculation of tyrosinase inhibition:

3. results of the experiment

TABLE 3 comparison of antioxidant Capacity of motherwort before and after fermentation with Bifidobacterium

As shown in Table 3 above, the DPPH radical scavenging ability of motherwort after fermentation with Bifidobacterium was not significantly improved, but was comparable to 0.03mg/ml V _E Has equivalent antioxidant effect. Therefore, the motherwort fermented primary pulp has stronger oxidation resistance.

Meanwhile, the Chinese herbal medicine motherwort herb has low tyrosinase inhibition capability, but after fermentation, the tyrosinase inhibition rate is 1.24 times that before fermentation. The tyrosinase inhibition ability obtained after fermentation is equivalent to the tyrosinase inhibition rate of 0.1mg/ml kojic acid. It can be seen that bifidobacterium bifidum fermentation has a higher effect of improving the tyrosinase inhibition ability of motherwort.

Experimental example 2 Effect of different dilution factors of Bifidobacterium bifidum and motherwort fermentation protoplasm on DPPH free radical scavenging ability

As can be seen from fig. 1, the clearance rate of bifidobacterium bifidum and motherwort fermentation raw stock DPPH is not significantly changed when diluted by 2 times, 4 times and 8 times, and is always higher than the half-inhibitory concentration, which indicates that the effect of reducing the concentration on the antioxidant effect of the motherwort fermentation raw stock is small. Meanwhile, when diluted 16 times, the DPPH free radical clearance rate is reduced but is equal to 0.02mg/ml V _E Has the same antioxidant effect. Therefore, the bifidobacterium bifidum and motherwort fermented primary pulp has good oxidation resistance and strong oxidation resistance stability.

Experimental example 3 comparison with DPPH free radical scavenging ratio of commercial raw material

As can be seen from FIG. 2, the difference of DPPH free radical scavenging rate between the motherwort herb fermentation raw stock and VE, VC and ergothioneine is not significant and reaches more than 70%. The common antioxidants astaxanthin, coenzyme Q10, fullerene and carnosine are far lower than those of the motherwort fermentation raw stock, which shows that the motherwort fermentation raw stock has good oxidation resistance.

Experimental example 4 Effect of Bifidobacterium bifidum and motherwort fermented raw stock on tyrosinase inhibition ability by different dilution times

From fig. 3, it can be seen that the difference of the clearance rate change of the bifidobacterium bifidum and motherwort fermentation raw stock tyrosinase inhibition rate is not significant when diluted by 2 times and is higher than the half inhibition concentration. Meanwhile, when diluted by 2 times, the tyrosinase inhibition rate is equivalent to the whitening effect of 0.05mg/ml kojic acid. Along with the increase of the dilution factor, the tyrosinase inhibition rate is gradually reduced, and when the dilution factor is more than 8 times, the tyrosinase inhibition rate basically tends to be stable. Therefore, the bifidobacterium bifidum and motherwort fermented primary pulp has strong whitening capacity, the influence on the whitening performance can be ignored by diluting 2 times, and the cost can be saved in the actual production.

Experimental example 5 compares the tyrosinase inhibition rate with that of the commercial whitening raw material

As can be seen from FIG. 4, the inhibition ability of tyrosinase of the motherwort herb fermentation raw stock is far higher than that of arbutin, 1% of nicotinamide, 2% of nicotinamide, commercially available Saururus chinensis plant extract, motherwort herb plant extract, broom cypress plant extract and 1.5% of sapphire, and the difference is very obvious. The effect of the fermented motherwort primary pulp is equivalent to that of the highest addition concentration of 5% of nicotinamide in cosmetics, and the fermented motherwort primary pulp has a good whitening effect.

Example 4 whitening double-layer base essence added with motherwort Chinese herbal medicine fermentation raw stock

Comprises the following components:

group A

Group B

Group C

1 part of sodium hydroxide

1 part of deionized water

Group D

0.15 portion of sodium citrate

0.19 part of citric acid

1 part of deionized water

Group E

The preparation method comprises the following steps:

(1) Mixing group A uniformly in advance for later use;

(2) Scattering the group B powder into water, uniformly stirring, heating to 80-82 ℃, completely dissolving uniformly, homogenizing for 1-2 minutes, and defoaming for later use;

(3) Dissolving group C and D in advance for later use;

(4) Cooling group B to below 40 deg.C, sequentially adding groups C, D and E, and stirring;

(5) Group A was added slowly to (4).

Example 5 whitening face cream added with motherwort herb fermented primary pulp

Comprises the following components:

group A

Group B

Group C

Motherwort fermented raw stock 8 parts

Group D

Spectrastat PHL 1 part

The preparation method comprises the following steps:

(1) Mixing and heating the group A to 80-85 ℃, stirring until the solid is completely melted into liquid, and keeping the temperature for later use;

(2) Mixing group B, heating to 80-85 deg.C, stirring until the solid is completely melted into liquid, and keeping the temperature for use;

(3) Slowly adding the group A into the group B under weak and medium-speed stirring, and homogenizing at medium speed for 1-3 minutes;

(4) Adding the group C and the group D into the mixture obtained in the step (3), and stirring at a low speed for 5 minutes;

experimental example 6 Patch test on human body

Test method

The selected area is 40mm ² And qualified spot test equipment with the depth of about 1 mm. And (3) test treatment: 0.025mL of the test cosmetic was placed in the chamber of the patch tester, and the patch tester with the test substance applied thereto was applied to the curved side of the forearm of the subject with a hypoallergenic tape, and gently pressed with the palm of the hand to apply the test cosmetic evenly to the skin for 24 hours. Blank control treatment is set for each test, no substance is placed in the control spot tester hole, and other processes are the same as the testAnd (4) processing the groups. The skin reaction was observed according to the standard of table 1 30min (after disappearance of the indentation), 24h and 48h after removal of the test substance plaque test device, respectively, and the observation results were recorded.

Skin closed patch test skin reaction degree grading standard is as follows:

"-" = negative reaction;

"±" = suspicious reaction, only weak erythema;

"+" = weak positive reaction (erythema reaction); erythema, infiltration, edema, and possibly pimples;

"++" = strong positive reaction (herpetic response); erythema, infiltration, edema, papules, herpes; the reaction may be beyond the test area;

"+ + + +" = very strong positive reaction (fusogenic herpes response); obvious erythema, severe infiltration, edema, and fusional herpes; the reaction was beyond the test area.

The judgment standard of the human body patch test is as follows: the number of plus or minus adverse reactions in 30 subjects is more than 5, or the number of plus or minus adverse reactions is more than 2, or any skin adverse reaction above 1 plus or minus plus is judged to have adverse reactions on human body, otherwise, the human body is judged to have no adverse reactions.

As can be seen from table 4: the obtained test results do not produce adverse reactions, which indicates that the motherwort herb fermented raw pulp provided by the invention has safety and does not bring adverse reactions to human bodies.

TABLE 4 test results of the small arm patch test

The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for some of the features thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.

Claims

1. A motherwort herb fermented protoplasm is characterized in that motherwort herb powder, bacterial liquid and water are mixed to obtain an initial system, the initial system is subjected to fermentation culture to obtain fermentation liquor, and the fermentation liquor is subjected to sterilization and centrifugation to obtain supernatant, so that the motherwort herb fermented protoplasm is obtained.

2. The motherwort fermented puree according to claim 1, wherein the preparation method comprises the following steps:

(1) Preparing materials: removing impurities from herba Leonuri, cleaning, oven drying, and pulverizing;

(2) And (3) strain culture: activating the strain to obtain activated bacterial liquid; purifying the strain to obtain a purified strain; performing enlarged culture on the strains to obtain zymocyte liquid;

(3) And (3) fermentation: mixing the zymophyte liquid, the motherwort powder and water according to the proportion, and fermenting to obtain fermentation liquid;

(4) And (3) purification: filtering the obtained fermentation liquor, sterilizing the filtrate to inactivate the strains, centrifuging the sterilized fermentation filtrate, removing the precipitate, and collecting the supernatant, namely the traditional Chinese medicine composition fermentation raw stock provided by the invention.

3. The fermented motherwort syrup according to claim 2, wherein in the step (1), the motherwort raw material is crushed to 100 mesh.

4. The motherwort fermented puree according to claim 2, wherein in the step (2), the strain culture specifically comprises:

and (3) strain amplification culture: inoculating the purified strain to a BS medium (C), (C)pH 7.0), standing in an incubator at 37 deg.C, and obtaining zymocyte liquid with strain in logarithmic phase and concentration of (10) when OD =0.6-1.0 ⁶ -10 ⁸ CFU/mL); the preparation concentration is 10 ⁶ ～10 ⁸ The pH value of the cfu/mL zymocyte liquid is adjusted to 6.5-7.5.

5. The motherwort fermented puree according to claim 2, wherein in the step (3), the fermentation conditions are as follows: fermenting at 37 deg.C for 40 hr.

6. The motherwort fermented puree according to claim 2, wherein in the step (4), the specific purification process comprises the following steps: filtering the obtained fermentation liquor, autoclaving the filtrate at 121 deg.C for 30min to inactivate the strain, centrifuging the sterilized fermentation filtrate at 12000r/min and centrifugation radius of 9cm for 30min, removing precipitate, and collecting supernatant to obtain herba Leonuri fermented raw stock.

7. The motherwort fermented raw stock according to claim 2, wherein the ratio of the amount of the zymophyte liquid, the amount of the motherwort and the amount of water in the motherwort fermented raw stock is 0-30 mL: 10-80 g: 50-600 g.

8. The motherwort fermented puree according to claim 2, wherein the bacterial species in the bacterial liquid are bifidobacterium bifidum.

9. The use of the fermented motherwort puree of any one of claims 1 to 8 in cosmetics, wherein the cosmetics comprise the fermented motherwort puree in an amount of 5%.

10. The use of the motherwort fermented puree in cosmetics according to claim 9, wherein the cosmetics comprise astringent, lotion, cream, mask, cleanser or BB cream.