CN115919696A - Composition with effects of removing dandruff and relieving itching, preparation method and application - Google Patents
Composition with effects of removing dandruff and relieving itching, preparation method and application Download PDFInfo
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- CN115919696A CN115919696A CN202310185519.0A CN202310185519A CN115919696A CN 115919696 A CN115919696 A CN 115919696A CN 202310185519 A CN202310185519 A CN 202310185519A CN 115919696 A CN115919696 A CN 115919696A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention provides a composition with the effects of removing dandruff and relieving itching, a preparation method and application thereof, wherein the composition with the effects of removing dandruff and relieving itching comprises tea tree essential oil and prinsepia utilis royle oil; the composition with dandruff removing and itching relieving effects also comprises lavender essential oil and/or litsea cubeba oil. According to the invention, tea tree essential oil, prinsepia utilis royle oil, lavender essential oil and/or litsea cubeba oil are creatively applied to the preparation of the composition with the effects of removing dandruff and relieving itching, the composition with the effects of removing dandruff and relieving itching is safe, efficient, low in toxicity and environment-friendly, and is safer compared with traditional chemical bacteriostatic agents and antibiotics, and the problems of environmental pollution and the occurrence of drug-resistant bacteria are avoided.
Description
Technical Field
The invention belongs to the technical field of daily chemicals, and relates to a composition with effects of removing dandruff and relieving itching, a preparation method and application thereof.
Background
The problems of dandruff and scalp itching often bring negative influence to the work and life of people. Malassezia (Malassezia) is widely recognized as the major pathogenic microorganism causing dandruff, scalp itching, and other problems. Malassezia is a lipidophilic fungus that does not have the ability to synthesize fatty acids by itself. However, malassezia bacteria have the ability to secrete lipases, phospholipases and sphingomyelin hydrolases, allowing them to use the lipids produced by the host to produce free fatty acids for their own use. Therefore, a large amount of unsaturated fatty acid, free fatty acid and indole substances are metabolized in the growth and propagation processes of the Chinese herbal medicine, and the substances can stimulate the skin, damage the skin barrier, interfere the differentiation of sebaceous gland cells and accelerate the proliferative metabolism of cutin, so that a large amount of surface cells are abnormally dropped, and the problem of dandruff is caused. Its mass propagation can induce skin to secrete more oil and fat, and cause skin barrier damage, further cause skin inflammation, scalp itching, alopecia, etc., and form malignant circulation. In conclusion, the malassezia bacteriostat and the anti-inflammatory agent are effective ways for removing dandruff and relieving itching.
Many malassezia inhibitors are currently used in personal care products, such as: zinc Pyrithione (ZPT), ketoconazole (Ketoconazole), climbazole (Climbazole), pyridone ethanolamine (OCT), hexamidine bis (hydroxyethyl) sulfonate, undecene derivatives and the like all have good malassezia antibacterial activity and have a good anti-dandruff effect, but these substances are environmentally unfriendly, strongly irritant or antibiotic substances, and easily cause the problems of allergy, environmental pollution, drug-resistant bacteria generation and the like, so that the search for a natural and safe malassezia antibacterial agent is a problem to be solved urgently at present.
The plant essential oil is a natural antibacterial agent, is used as a metabolite of plants, has the advantages of high safety, low toxicity, environmental friendliness and difficulty in occurrence of microbial drug resistance, and has great development potential in the field of personal care products. However, when a single essential oil is used as a bacteriostatic agent, the problem of large dosage is caused, and the problems of potential sensitization, odor irritation, single odor and the like exist, so that the application of the essential oil in the field of personal care products is limited.
CN105997596A discloses an antidandruff composition with synergistic effect of plant essential oil, which shows that the plant essential oil and ZPT have synergistic bacteriostatic effect, but do not have anti-inflammatory and antipruritic effects. The plant essential oil mainly contains terpenes, phenols, aldehydes and alcohols, but the species and content proportion of substances contained in different plant essential oils are different, and the antibacterial mechanisms of the plant essential oils are different, so that the compound essential oil can show synergistic antibacterial activity, thereby improving the antibacterial efficiency of the essential oil and the odor of products.
The study of Wanggui et al shows that after the litsea cubeba essential oil and the cinnamon essential oil are prepared according to the proportion of 2.
CN112315835A discloses an oil-controlling and dandruff-removing composition, wherein the composition contains zinc pyrithione, melaleuca alternifolia oil (tea tree oil), saccharide isomerate, caprylyl glycine and eucalyptus oil, which has dandruff-removing and oil-controlling effects, but does not have anti-inflammatory and antipruritic effects, and ZPT is still added as an inhibitor for inhibiting malassezia.
Therefore, the existing anti-dandruff nursing products containing malassezia inhibitors in the market have the problems of being environment-friendly, strong in sensitization, easy to generate drug resistance and the like. Along with the continuous improvement of the living standard of people and the continuous improvement of the requirements on health and environment, the research and development of a itching-relieving and dandruff-removing product which has strong curative effect, is environment-friendly, mild and non-irritating is very meaningful.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a composition with the efficacy of removing dandruff and relieving itching, and a preparation method and application thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a composition with anti-dandruff and anti-itching effects, which comprises tea tree essential oil and prinsepia utilis royle oil; the composition with dandruff removing and itching relieving effects also comprises lavender essential oil and/or litsea cubeba oil.
The tea tree essential oil is extract of Australian tea tree (or Melaleuca alternifolia), and has effects of killing bacteria, relieving inflammation, astringing follicular orifices, treating common cold, cough, rhinitis, asthma, and improving dysmenorrhea, menoxenia and genital infection. It is suitable for oily and acne skin, and can be used for treating suppurative wound, burn, sunburn and dandruff.
The Prinsepia utilis Royle ex Fr.T. Chen has a long history of eating and medicinal use among Yunnan folks, the kernels of the Prinsepia utilis Royle ex Fr are rich in natural oil, the natural vegetable oil has the effects of resisting inflammation, preserving moisture, improving skin smoothness and improving skin toughness, and has great development value in the field of personal care products, and researches of Cao Roxburgh anoectochilus ex Fr and other people find that the Prinsepia utilis Royle ex Fr oil can effectively inhibit the auricle swelling of mice, and the highest inhibition rate is 23.57% ([ Cao Roxburgh, and Lisheng, liu Yinhua, and the like; experimental researches on the gel anti-inflammatory and antipruritic effects of the Prinsepia utilis Royle ex Fr et T. [ J ]. Yunnan TCM journal of 2021, 42 (02): 64-66). Therefore, the prinsepia utilis royle oil has obvious anti-inflammatory and detumescence effects, but the anti-inflammatory and itching-relieving mechanism of the prinsepia utilis royle oil is not analyzed in the research.
The lavender essential oil has the effects of clearing away heat and toxic materials, cleaning skin, removing freckles, whitening, balancing grease secretion, disinfecting and resisting bacteria, astringing pores and the like, and is a common additive component of a cleaning and nursing product.
The pungent litse fruit oil is extracted from pungent litse fruit, contains a large amount of citral, has fragrant and sweet fruity flavor, and also has antibacterial effect on Staphylococcus aureus, typhoid bacillus, pseudomonas aeruginosa and Malassezia.
According to the invention, tea tree essential oil, prinsepia utilis royle oil, lavender essential oil and/or litsea cubeba oil are creatively applied to the preparation of the composition with the effects of removing dandruff and relieving itching, the composition with the effects of removing dandruff and relieving itching is safe, efficient, low in toxicity and environment-friendly, and is safer compared with traditional chemical bacteriostatic agents and antibiotics, and the problems of environmental pollution and the occurrence of drug-resistant bacteria are avoided. The composition can effectively inhibit the proliferation of malassezia bacteria after being added for 5min at the adding amount of 1wt%, and the bacteriostasis rate can reach 85%; the composition can also obviously inhibit the secretion of TNF-alpha and IL-6 in cells, thereby achieving the effects of resisting inflammation, relieving itching and reducing scalp barrier injury.
Preferably, the composition with the effects of removing dandruff and relieving itching further comprises lavender essential oil or litsea cubeba oil.
The composition with the effects of removing dandruff and relieving itching also comprises lavender essential oil or litsea cubeba oil, wherein when the composition with the effects of removing dandruff and relieving itching also comprises the lavender essential oil, the lavender essential oil can be combined with tea tree essential oil for application, the lavender essential oil and the tea tree essential oil supplement each other and have a better bacteriostatic effect than a single component, in addition, the combination of the lavender essential oil and the tea tree essential oil is combined with prinsepia utilis royle oil for application, the prinsepia utilis royle oil can enhance the bacteriostatic effect of the other two components, and the effects of the three on inhibiting the proliferation of malassezia;
when the composition with the effects of removing dandruff and relieving itching also comprises litsea cubeba oil, the litsea cubeba oil and tea tree essential oil can be combined and applied, the two components supplement each other, the synergistic effect is achieved in the aspect of bacteriostasis, the prinsepia utilis royle oil can also enhance the bacteriostasis of the litsea cubeba oil and the tea tree essential oil, and the effect of inhibiting the proliferation of malassezia is stronger when the three components are combined and applied.
When the composition with the effects of removing dandruff and relieving itching also comprises lavender essential oil or litsea cubeba oil, the composition with the effects of removing dandruff and relieving itching has obvious anti-inflammatory and bacteriostatic effects.
Preferably, the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil is 1 (0.5-4.5).
The specific value of (0.5-4.5) can be selected from 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 or 4.5, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the mass ratio of the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil is 1 (0.5-4.5) to 0.5-4.
Specific values of (0.5-4.5) can be selected from 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 or 4.5, etc.
The specific value of (0.5-4) can be selected from 0.5, 1, 1.5, 2, 2.5, 3, 3.5 or 4, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the litsea cubeba essential oil is 1 (0.5-4.5) to 0.5-4.
The specific value of (0.5-4.5) can be selected from 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4 or 4.5, etc.
The specific value of (0.5-4) can be selected from 0.5, 1, 1.5, 2, 2.5, 3, 3.5 or 4, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
When the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil is 1 (0.5-4.5), the composition has a stronger inhibiting effect on malassezia. When the mass ratio of the tea tree essential oil, the prinsepia utilis royle oil and the litsea cubeba essential oil is specially selected from 1 (0.5-4.5) to 0.5-4) or the mass ratio of the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil is specially selected from 1 (0.5-4.5) to 0.5-4), the antibacterial effect of the composition is further enhanced.
In a second aspect, the present invention provides a method for preparing the composition with anti-dandruff and anti-itching effects according to the first aspect, wherein the preparation method comprises the following steps:
and uniformly mixing the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil or uniformly mixing the tea tree essential oil, the prinsepia utilis royle oil and the litsea cubeba oil to prepare the composition with the effects of removing dandruff and relieving itching.
According to the preparation method of the composition with the effects of removing dandruff and relieving itching, the composition with the effects of removing dandruff and relieving itching can be prepared only by simply mixing the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil or the tea tree essential oil, the prinsepia utilis royle oil and the litsea cubeba oil; therefore, the composition has simple preparation process and is easy to be industrially amplified.
Preferably, the preparation method of the tea tree essential oil comprises the following steps:
pulverizing Melaleuca alternifolia, sieving, soaking in water, heating to boil, refluxing, and collecting distillate; adding sodium chloride into the distillate, standing, and collecting an oil phase layer to obtain the tea tree essential oil;
preferably, the mass of the added water is 5-20 times of the mass of the leaf of the melaleuca alternifolia;
the specific value of 5-20 can be selected from 5, 7, 9, 11, 13, 15, 17, 19 or 20, etc.
Other specific values within the above ranges can be selected, and are not described in detail herein.
Preferably, the mass of the added water is 10-18 times of the mass of the leaf of the melaleuca alternifolia.
The specific value of 10-18 can be selected from 10, 11, 12, 13, 14, 15, 16, 17 or 18, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the preparation method of the lavender essential oil comprises the following steps:
pulverizing stems and leaves of Lavender, sieving, soaking in water, heating to boil, refluxing, and collecting distillate; adding sodium chloride into the distillate, standing, and collecting oil phase layer to obtain lavender essential oil;
preferably, the mass of the added water is 10-30 times of that of the lavender stems and leaves.
The specific value of 10-30 can be selected from 10, 13, 16, 19, 21, 24, 27 or 30, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the preparation method of the litsea cubeba oil comprises the following steps:
pulverizing fructus Litseae, sieving, soaking in water, heating to boil, refluxing, and collecting distillate; adding sodium chloride into the distillate, standing, and collecting oil phase layer to obtain pungent litse fruit oil;
preferably, the mass of the added water is 5-20 times of that of the litsea cubeba fruits;
the specific value of 5-20 can be selected from 5, 7, 9, 11, 13, 15, 17, 19 or 20, etc.
Other specific values within the above ranges can be selected, and are not described in detail herein.
Preferably, the mass of the added water is 8-15 times of that of the litsea cubeba fruits.
The specific numerical value in the range from 8 to 15 can be selected from 8, 9, 10, 11, 12, 13, 14 or 15, and the like.
Other specific values within the above ranges can be selected, and are not described in detail herein.
Preferably, the mesh number of the sieved mesh is independently 10-100 mesh; more preferably, the mesh number of the sieved mesh is independently 10 to 40 mesh;
the specific numerical value in the 10-100 meshes can be 10 meshes, 20 meshes, 30 meshes, 40 meshes, 50 meshes, 60 meshes, 70 meshes, 80 meshes, 90 meshes, 100 meshes and the like.
The specific numerical value in the 10-40 meshes can be selected from 10 meshes, 15 meshes, 20 meshes, 25 meshes, 30 meshes, 35 meshes, 40 meshes and the like.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the soaking time is independently 8-20h;
the specific value of 8-20h can be selected from 8h, 10h, 12h, 14h, 16h, 18h or 20h, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the boiling reflux time is independently 1-4h;
the specific value of 1-4h can be selected from 1h, 1.3h, 1.6h, 1.9h, 2.2h, 2.5h, 2.8h, 3.1h, 3.4h, 3.7h or 4h, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the heating to boiling reflux and the collection of distillate are independently repeated for 1-3 times;
the specific value of the 1-3 times can be selected from 1 time, 2 times and 3 times.
Preferably, the concentration of sodium chloride in the distillate is independently 1-5wt%;
specific values among the 1-5wt% can be selected from 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, or the like.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the standing is independently 8-20h;
the specific value of 8-20h can be selected from 8h, 10h, 12h, 14h, 16h, 18h or 20h, etc.
Other specific point values within the above numerical ranges can be selected, and are not described in detail herein.
Preferably, the operation of adding anhydrous sodium sulfate to remove residual moisture is independently included after the oil phase layer is collected;
preferably, the anhydrous sodium sulfate is added in an amount of 5 to 25wt% of the oil phase layer liquid independently;
specific values of 5 to 25wt% can be selected from 5wt%, 8wt%, 11wt%, 13wt%, 16wt%, 19wt%, 22wt%, 23wt%, 25wt%, etc.
Other specific values within the above ranges can be selected, and are not described in detail herein.
Preferably, the operation of filtering by using a sterilizing filter membrane is independently further included after the addition of anhydrous sodium sulfate to remove residual water.
In a third aspect, the invention provides the composition with the efficacy of removing dandruff and relieving itching or the preparation method of the second aspect, and the composition or the preparation method is applied to daily care products for removing dandruff, relieving itching and inhibiting bacteria.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, tea tree essential oil, prinsepia utilis royle oil, lavender essential oil and/or litsea cubeba oil are creatively applied to the preparation of the composition with the effects of removing dandruff and relieving itching, the composition with the effects of removing dandruff and relieving itching is safe, efficient, low in toxicity and environment-friendly, is safer compared with traditional chemical bacteriostatic agents and antibiotics, and cannot cause the problem of environmental pollution and cause the appearance of drug-resistant bacteria. The composition can effectively inhibit the proliferation of malassezia bacteria after being added for 5min at the adding amount of 1wt%, and the bacteriostasis rate can reach 85%; the composition can also obviously inhibit the secretion of TNF-alpha and IL-6 in cells, thereby achieving the effects of resisting inflammation, relieving itching and reducing scalp barrier injury.
2. According to the preparation method of the composition with the effects of removing dandruff and relieving itching, the composition with the effects of removing dandruff and relieving itching can be prepared only by simply mixing the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil or the tea tree essential oil, the prinsepia utilis royle oil and the litsea cubeba oil; therefore, the composition has simple preparation process and is easy to be industrially amplified.
Drawings
FIG. 1 is an appearance diagram of the composition with anti-dandruff and anti-itch effects prepared in example 1.
FIG. 2 is a graph showing the results of the inhibition zones in comparative examples 1 to 6, positive control group and negative control group in test example 1.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitation of the present invention.
Modified Dixon agar medium referred to below is a product purchased from Gibco; DMEM basal medium, DMEM complete medium were purchased from Gibco products; MTT reagent is a product purchased from solibao; dexamethasone was purchased from Shanghai leaf Biotech Co., ltd; the ELISA kit is purchased from Saimeishafil; LPS is a product number L4516-1MG purchased from Sigma company; prinsepia utilis Royle oil is from Yunnan England Biotechnology limited.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.
Preparation example 1
The preparation example provides Australia tea tree essential oil, and the preparation method of the Australia tea tree essential oil comprises the following steps:
weighing 10 parts of dried melaleuca alternifolia dry leaves, crushing, sieving with a 20-mesh sieve, soaking in 15 times of purified water by weight of leaf powder for 12 hours, boiling and refluxing for 3 hours, collecting distillate, then boiling and refluxing for 2 hours with 10 times of purified water by weight of leaf powder, collecting distillate, combining the two collected distillates, adding 3% sodium chloride of the distillate, placing in a separating funnel, and standing for 12 hours. Discharging the lower layer of water, collecting the upper layer of essential oil, adding anhydrous sodium sulfate according to 15% of the mass of the essential oil to remove water in the essential oil, filtering the collected essential oil by using a 0.22 mu m sterilizing filter membrane, collecting the filtrate, and subpackaging to obtain the Australian tea tree essential oil.
Preparation example 2
The preparation example provides lavender essential oil, and the preparation method of the lavender essential oil comprises the following steps:
weighing 10 parts of lavender stems, leaves and flowers, crushing, sieving with a 30-mesh sieve, soaking in 20 times of purified water by weight of dried flowers for 12 hours, boiling and refluxing for 4 hours, collecting distillate, then taking 20 times of purified water by weight of dried flowers, boiling and refluxing for 1 hour, collecting distillate, combining the two collected distillates, adding 3% of sodium chloride of the distillate, placing in a separating funnel, and standing for 12 hours. Discharging lower layer water, collecting upper layer essential oil, adding anhydrous sodium sulfate according to 20% of the essential oil by mass to remove water in the essential oil, filtering the collected essential oil with 0.22 μm sterilizing filter membrane, collecting filtrate, and packaging to obtain lavender essential oil.
Preparation example 3
The preparation example provides litsea cubeba oil, and the preparation method of the litsea cubeba oil comprises the following steps:
weighing 10 parts of dried litsea cubeba fruits, crushing, sieving with a 50-mesh sieve, soaking in purified water 15 times the weight of dry powder for 12 hours, boiling and refluxing for 4 hours, collecting distillate, then boiling and refluxing for 2 hours in purified water 15 times the weight of leaf powder, and collecting distillate. The two collected distillates are combined and placed in a separating funnel to stand for 12 hours. Discharging lower layer water, collecting upper layer essential oil, adding anhydrous sodium sulfate at an amount of 20% of the essential oil by mass to remove water in the essential oil, filtering the collected essential oil with 0.22 μm sterilizing filter membrane, collecting filtrate, and packaging to obtain pungent litse fruit oil.
Example 1
The present example provides a composition with anti-dandruff and anti-itch effects, comprising: 35 parts of tea tree essential oil, 30 parts of prinsepia utilis royle oil and 35 parts of lavender essential oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the lavender essential oil is 1.
Example 2
The present example provides a composition with anti-dandruff and anti-itch effects, comprising: 18 parts of tea tree essential oil, 9 parts of prinsepia utilis royle oil and 73 parts of lavender essential oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the lavender essential oil is about 1.
Example 3
The present example provides a composition with anti-dandruff and anti-itch effects, comprising: 16.7 parts of tea tree essential oil, 75 parts of prinsepia utilis royle oil and 8.3 parts of lavender essential oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the lavender essential oil is about 1.
Example 4
The present example provides a composition with anti-dandruff and anti-itch effects, comprising: 20 parts of tea tree essential oil, 53.3 parts of prinsepia utilis royle oil and 26.7 parts of litsea cubeba oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the litsea cubeba oil is 1.7.
Example 5
The present example provides a composition with anti-dandruff and anti-itch effects, comprising: 18 parts of tea tree essential oil, 9 parts of prinsepia utilis royle oil and 73 parts of litsea cubeba oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the litsea cubeba oil is about 1.
Example 6
The present embodiment provides a composition with anti-dandruff and anti-itch effects, comprising: 16.7 parts of tea tree essential oil, 75 parts of prinsepia utilis royle oil and 8.3 parts of litsea cubeba oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the litsea cubeba oil is about 1.
Example 7
This example provides a composition with anti-dandruff and anti-itching effect, and the rest of the examples 1 are different only in that: the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil are replaced by 68 parts of tea tree essential oil, 16 parts of prinsepia utilis royle oil and 16 parts of lavender essential oil (the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the lavender essential oil is 1.
Example 8
This example provides a composition with anti-dandruff and anti-itch effects, and the remaining examples 4 differ only in that: the following components in part by weight were replaced with 20 parts of tea tree essential oil, 53.3 parts of prinsepia utilis royle oil, 26.7 parts of litsea cubeba oil (the mass ratio of tea tree essential oil, prinsepia utilis royle oil to litsea cubeba oil is 1.7.
Comparative example 1
The comparative example provides a composition with the effects of removing dandruff and relieving itching, which is different from the composition in example 1 only in that lavender essential oil is not contained in the composition, the parts by weight of the lavender essential oil are distributed to tea tree essential oil, and the rest is the same as the composition in example 1.
Comparative example 2
This comparative example provides a composition having anti-dandruff and anti-itch effects, which is different from example 1 only in that the composition does not contain tea tree essential oil, and the tea tree essential oil is distributed to lavender essential oil in parts by mass, and the rest is the same as example 1.
Comparative example 3
This comparative example provides a composition having anti-dandruff and antipruritic effects, which is different from example 1 only in that the composition does not contain prinsepia utilis royle oil, and the rest is the same as example 1.
Comparative example 4
The comparative example provides a composition with the effects of removing dandruff and relieving itching, which is different from the composition in example 4 only in that litsea cubeba oil is not contained in the composition, the parts by mass of the litsea cubeba oil are distributed to tea tree essential oil, and the rest is the same as the composition in example 4.
Comparative example 5
The comparative example provides a composition with the effects of removing dandruff and relieving itching, which is different from the composition in example 4 only in that tea tree essential oil is not contained in the composition, the tea tree essential oil is distributed to litsea cubeba oil in parts by mass, and the rest is the same as the composition in example 4.
Comparative example 6
This comparative example provides a composition having anti-dandruff and antipruritic effects, which is different from example 4 only in that the composition does not contain prinsepia utilis royle oil, and the rest is the same as example 4.
Test example 1
The test example evaluates the bacteriostatic effect of the compositions with the efficacy of removing dandruff and relieving itching prepared in the examples and the comparative examples.
1. The inhibition zones of the compositions prepared in examples 1 and 4 and comparative examples 1 to 6 were evaluated first, and the specific evaluation methods were as follows:
(1) Preparing a bacterial suspension: the frozen Malassezia species (ATCC 44344) was inoculated on olive oil Sasa medium under aseptic conditions, and placed in an incubator to be cultured at 36 ℃ for 6 days. Diluting activated Malassezia with sterilized normal saline to obtain 10 6 cfu/mL of bacterial suspension (used up within 6 hours).
(2) Sucking 0.1mL of the prepared bacterial suspension, uniformly coating the bacterial suspension on a culture medium, placing a sterilized Oxford cup in the middle of the flat plate, and then taking 0.2mL of a sample to be placed in the Oxford cup. The plate loaded with 1% ZPT solution was taken as a positive control, and the plate loaded with physiological saline was taken as a negative control.
(3) And (3) placing each group of culture medium in an incubator, culturing for 5d at 36 ℃, observing the growth condition of colonies and recording the size of a bacteriostatic zone. Image analysis was performed using Image-Pro-Plus 6.0 software. The results of the experiment are shown in table 1 and fig. 2.
2. The compositions prepared in examples 1 to 8 and comparative examples 3 and 6 were evaluated for the bacteriostatic ratio. The specific evaluation method comprises the following steps:
(1) Sterile PBS buffer was used to prepare a 10 concentration solution 6 CFU/mL of Malassezia (ATCC 44344) suspension; add 10. Mu.L of the bacterial suspension to a 1cm diameter pad of sterile dry filter paper and dry at 36 ℃ until use.
(2) The composition of each example or comparative example was filtered through a 0.22 μm sterile filter, and the filtrate was stored at 25 ℃ in a sealed and dark state. The composition prepared in each example or comparative example was added to a PBS buffer solution to prepare a concentration of 1wt%.
(3) And (3) adding the composition solution prepared in the step (2) into a 100g sterile bottle, adding a bacteria-staining filter paper sheet according to the proportion of 5 g/sheet, completely immersing the bacteria-staining carrier into the solution, and immediately timing for 5min. After timing is finished, adding the bacteria-infected filter paper sheet into 5mL PBS buffer solution, mixing uniformly, fully vibrating, washing the bacteria from the filter paper sheet carrier, diluting by 10 times, sequentially diluting by 5, 10 and 100 times, respectively taking 1mL bacterial liquid for each dilution, adding the bacterial liquid into a solid modified Dixon agar culture medium, carrying out inverted culture at 36 ℃ for 7d, and counting. Wherein 5mL of PBS buffer was used as a positive control group, and the same lot of modified Dixon agar medium was used as a negative control group.
(4) Counting colonies of each group of solid culture medium, calculating the average value of the number of the colonies of each group of sterile bottles, and calculating the bacteriostasis rate (the percentage of the colony count of the positive control group-the colony count of the experimental group)/the colony count of the positive control group is recorded as the bacteriostasis rate, and the result is shown in table 2.
TABLE 1
TABLE 2
| Group of | Bacteriostatic ratio (%) |
| Example 1 | 85.0 |
| Example 2 | 81.5 |
| Example 3 | 75.8 |
| Example 4 | 84.6 |
| Example 5 | 79.7 |
| Example 6 | 75.4 |
| Example 7 | 46.7 |
| Example 8 | 44.3 |
In Table 1, ND represents no inhibition zone detected.
As can be seen from the data in table 1, the comparative examples 1 and 2 lack one of lavender essential oil and tea tree essential oil respectively compared with the example 1, and the diameter of the inhibition zone is smaller than that of the example 1, which shows that the mutual matching and combination of the two components of the lavender essential oil and the tea tree essential oil have a synergistic effect in inhibiting malassezia;
compared with the example 4, the comparative examples 4 and 5 lack one of the litsea cubeba oil and the tea tree essential oil respectively, and the diameter of the inhibition zone is smaller than that of the example 4, which shows that the litsea cubeba oil and the tea tree essential oil have synergistic effect when being applied in the aspect of improving the malassezia inhibition effect.
Comparative example 3 or comparative example 6 lacked the prinsepia utilis royle oil as compared to example 1 or example 4, respectively, and the results show that: comparative example 3 and comparative example 6 show that the diameters of the inhibition zones are lower than those of the examples 1 and 4, respectively, and that the prinsepia utilis royle oil is added on the basis of the combination of the tea tree essential oil and the lavender essential oil or the tea tree essential oil and the litsea cubeba oil, and the prinsepia utilis royle oil can assist the inhibition component in improving the inhibition effect, so that the effect of remarkably improving the inhibition effect of the composition is achieved.
As can be seen from the data in Table 2, the composition of the present invention can achieve a bacteriostatic rate of about 85% after 5min treatment. Example 7 compared with example 1, the mass ratio of the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil is not in the preferable range 1 (0.5-4.5): within 0.5-4), the bacteriostatic rate is obviously lower than that of example 1, and the composition with the effects of removing dandruff and relieving itching has an excellent bacteriostatic effect when the mixture ratio of the three components is in the preferable range.
Compared with the example 4, the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil to the litsea cubeba oil is not in the preferred range of 1 (0.5-4.5): (0.5-4), the antibacterial rate is obviously lower than that of the example 4, and the antibacterial effect of the composition with the effects of removing dandruff and relieving itching is obviously improved when the mixture ratio of the three components is in the preferred range.
Test example 2
The test example evaluates the anti-inflammatory and antipruritic effects of the compositions prepared in examples 1 and 4, and the specific evaluation method comprises the following steps:
1. TNF-alpha and IL-6 inhibitory Activity assay
(1) Cell culture: culturing mouse macrophage RAW264.7 cell (purchased from Guangzhou Saiku biotechnology Co., ltd.) until the density exceeds 70%, discarding the old culture medium, adding 5mL PBS buffer solution to clean the cell, adding 4mL DMEM basal medium again, blowing the cell by using a pipetting gun to resuspend, transferring the cell into a 2mL centrifuge tube,centrifuging at 1000rpm for 4min, and discarding the supernatant; adding 1mL complete culture medium again, blowing, mixing, diluting by appropriate times, counting, and adjusting cell density to 8 × 10 according to counting result 4 cell/mL, inoculating cells into 96-well plates at a volume of 200. Mu.L per well, placing back into the incubator for incubation (37 ℃,5% 2 )。
(2) Grouping experiments: in the experiment, a blank control group, a negative control group, a positive control group (100 mg/mL of dexamethasone) and an experimental group are arranged, 3 non-cell holes are additionally arranged as zero-setting holes, the composition corresponding to example 1 in the experimental group is prepared into 2 concentration gradients (1: 0.0016 wt.%), (2: 0.0004 wt.%), the composition corresponding to example 4 is prepared into 2 concentration gradients (0.0125 wt.% (1); (2); 0.00156wt.%) by using DMEM basic culture medium, and 4 multiple holes are arranged under each concentration gradient;
(3) Cell safety test: and (3) testing the cell safety of the composition with the concentration of 0.0016wt% or 0.0125wt% by using an MTT method, taking out a 96-well plate 24 hours after the operation of the step (2), discarding the old culture medium, adding 200 mu L of DMEM basic culture medium into each well of a zero setting well and a blank control group, adding 200 mu L of sample prepared by the DMEM basic culture medium into each well of a cell activity experiment group, and distributing the plate according to 2 repeated wells of each group. Placing the mixture back into an incubator for culture (37 ℃,5% CO) 2 ) (ii) a And (3) taking out the 96-well plate 24h after the administration treatment, adding 20 mu L of MTT working solution (5 mg/mL) into each well, putting the plate back to the incubator for further culture for 4h, then removing the liquid in the wells, adding 150 mu L of DMSO into each well again, shaking for 10min, measuring the absorbance at 490nm wavelength, and calculating the relative activity of the cells: cell viability (%) = (cell activity assay absorbance-absorbance in wells set to zero)/(blank control absorbance-absorbance in wells set to zero) × 100%, and the results are shown in table 3.
(4) The administration scheme is as follows: and (3) taking out the 96-well plate 24 hours after the operation in the step (2), discarding the old culture medium, adding 180 mu L of DMEM basic culture medium into each well of the zero-setting well and the blank control group, adding 180 mu L of dexamethasone (100 mu g/mL) into each well of the positive control group, and adding 180 mu L of DMEM basic culture medium into each well of the experimental group. Placing the mixture back into an incubator for culture (37 ℃,5% CO) 2 ) And taking out 96-well plate after 1h, adding 20 μ L DMEM basal medium into each well of the zero-setting well and the blank control group, and adding a negative control group and a positive control groupAnd the experimental group was incubated with lipopolysaccharide LPS (20. Mu.L) per well (1. Mu.g/mL), followed by returning to the incubator.
(5) Collecting cell supernatants: and taking out the 96-well plate after administration for 24h, respectively collecting cell supernatants corresponding to the sample groups into a centrifuge tube, centrifuging at 1000rpm for 10min, collecting the supernatants, placing the supernatants into a 1.5mL centrifuge tube, and storing at-20 ℃ for later use.
(6) The concentrations of TNF-. Alpha.and IL-6 of interest in the cell supernatants were determined according to the ELISA kit instructions.
TABLE 3
| Group of | Concentration (wt%) | Cell viability (%) |
| Example 1 | 0.0016 | 99.4 |
| Example 4 | 0.0125 | 93.035 |
TABLE 4
As can be seen from the data in Table 3, the cell viability of the composition of example 1 was close to 90% at a concentration of 0.0016wt% or less, and the composition was tested at a concentration of 0.0016wt% or less; the cell viability of the composition described in example 4 is close to 90% at concentrations not exceeding 0.0125 wt.%, and the composition can be tested at concentrations not exceeding 0.0125wt%
As can be seen from the data in Table 4, the experimental groups with different concentrations corresponding to the compositions with the efficacy of removing dandruff and relieving itching in examples 1 and 4 have lower TNF-alpha and IL-6 than the negative control group, which indicates that the compositions with the efficacy of removing dandruff and relieving itching can reduce the activity of secreting TNF-alpha and IL-6 by cells, thereby playing the roles of resisting inflammation and relieving itching.
When the concentration of the composition described in example 1 is 0.0016wt%, the IL-6 result is close to that of dexamethasone in the positive control group, and when the addition amount of the composition described in example 4 is 0.00156wt%, the TNF-alpha result is close to that of the dexamethasone in the positive control group, which shows that the composition with the functions of removing dandruff and relieving itching can achieve good anti-inflammatory activity when being added in a trace amount.
Test example 3
The compositions obtained in examples 1 to 8 were evaluated for safety. The specific evaluation method comprises the following steps: the test was performed according to the human skin patch test method in the seventh chapter of human safety test methods in the cosmetic safety technical Specification 2015 edition. (1) Selecting female volunteers 18-60 years old according with experimental requirements, and excluding the following criteria: (a) Those who use antihistamines for nearly one week or immunosuppressants for nearly one month; (b) In the last two months, any anti-inflammatory drug is applied to the tested part; (c) a subject who has a clinical non-cure for an inflammatory skin disease; (d) insulin-dependent diabetic patients; (e) Patients suffering from asthma or other chronic respiratory diseases undergoing therapy; (f) subjects receiving anti-cancer chemotherapy within approximately 6 months; (g) patients with immunodeficiency or autoimmune disease; (h) lactating or pregnant women; (i) Bilateral mastectomy and bilateral underarm lymph node resection; (n) determining whether the skin area to be tested is affected by scar, pigment, atrophy, nevus flammeus or other blemishes; (o) participation in other clinical trial investigators; (p) those with a high predisposition; (q) non-volunteer participants or those who are unable to complete the prescribed content on trial. (2) one group for each example, 10 volunteers in each group; the selected area is not more than 50mm 2 And qualified spot test equipment with the depth of about 1 mm. The composition solution prepared in each examplePut into a chamber of a spot tester, and the amount of the solution is about 0.025g (solvent is GTCC, and the concentration of the test substance is 1 wt%). Control wells were blanked with caprylic/capric triglyceride (GTCC, available from Shanghai Helin commerce, inc.). The spot test device with the test substance is applied to the back or the forearm curve of the subject with hypoallergenic tape, and is applied to the skin uniformly by pressing with the palm for 24h. (3) Skin reactions were observed at 30min (after disappearance of the indentation), 24h, and 48h after removal of the test article plaque test, respectively, according to the criteria of table 5. And evaluated according to the criteria of table 5.
TABLE 5
The result shows that the composition with the effects of removing dandruff and relieving itching, which is prepared by the invention, is safe and non-irritant at the concentration of not more than 1wt%, and has higher use value.
The applicant states that the invention is illustrated by the above examples, but the invention is not limited to the above examples, i.e. it is not intended that the invention must rely on them to be practiced. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (10)
1. A composition with the effects of removing dandruff and relieving itching is characterized by comprising tea tree essential oil and prinsepia utilis royle oil; the composition with dandruff removing and itching relieving effects also comprises lavender essential oil and/or litsea cubeba oil.
2. The composition with anti-dandruff and antipruritic effects according to claim 1, wherein the composition with anti-dandruff and antipruritic effects further comprises lavender essential oil or litsea cubeba oil;
preferably, the mass ratio of the tea tree essential oil to the prinsepia utilis royle oil is 1 (0.5-4.5).
3. The composition with the effects of removing dandruff and relieving itching as claimed in claim 1 or 2, wherein the mass ratio of the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil is 1 (0.5-4.5) to (0.5-4).
4. The composition with the effects of removing dandruff and relieving itching as claimed in any one of claims 1 to 3, wherein the mass ratio of the tea tree essential oil, the prinsepia utilis royle oil and the litsea cubeba essential oil is 1 (0.5-4.5) to (0.5-4).
5. The method for preparing the composition with the efficacy of removing dandruff and relieving itching according to any one of claims 1 to 4, wherein the preparation method comprises the following steps:
and uniformly mixing the tea tree essential oil, the prinsepia utilis royle oil and the lavender essential oil or uniformly mixing the tea tree essential oil, the prinsepia utilis royle oil and the litsea cubeba oil to prepare the composition with the effects of removing dandruff and relieving itching.
6. The method for preparing a composition with anti-dandruff and antipruritic effects according to claim 5, wherein the tea tree essential oil is prepared by a method comprising:
pulverizing Melaleuca alternifolia, sieving, soaking in water, heating to boil, refluxing, and collecting distillate; adding sodium chloride into the distillate, standing, and collecting an oil phase layer to obtain the tea tree essential oil;
preferably, the mass of the added water is 5 to 20 times of the mass of the leaf of the phyllanthus alternatus;
preferably, the mass of the added water is 10-18 times of the mass of the leaf of the melaleuca alternifolia.
7. The method for preparing the composition with the effects of removing dandruff and relieving itching according to claim 5 or 6, wherein the method for preparing the lavender essential oil comprises the following steps:
pulverizing stems and leaves of Lavender, sieving, soaking in water, heating to boil, refluxing, and collecting distillate; adding sodium chloride into the distillate, standing, and collecting oil phase layer to obtain lavender essential oil;
preferably, the mass of the added water is 10-30 times of that of the lavender stems and leaves.
8. The method for preparing the composition with the efficacy of removing dandruff and relieving itching according to any one of claims 5 to 7, wherein the method for preparing the litsea cubeba oil comprises the following steps:
pulverizing fructus Litseae, sieving, soaking in water, heating to boil, refluxing, and collecting distillate; adding sodium chloride into the distillate, standing, and collecting oil phase layer to obtain pungent litse fruit oil;
preferably, the mass of the added water is 5-20 times of the mass of the litsea cubeba fruits;
preferably, the mass of the added water is 8-15 times of that of the litsea cubeba fruits.
9. The method for preparing a composition with dandruff-removing and antipruritic effects according to any one of claims 6 to 8, wherein the mesh number of the sieved screen is independently 10 to 100 mesh; more preferably, the mesh number of the sieved mesh is independently 10 to 40 mesh;
preferably, the soaking time is independently 8-20h;
preferably, the boiling reflux time is independently 1-4h;
preferably, the heating to boiling reflux and the collection of distillate are independently repeated for 1-3 times;
preferably, the concentration of sodium chloride in the distillate is independently 1-5wt%;
preferably, the standing is independently 8-20h;
preferably, the operation of adding anhydrous sodium sulfate to remove residual moisture is independently included after the oil phase layer is collected;
preferably, the addition amount of the anhydrous sodium sulfate is independently 5 to 25wt% of the oil phase layer liquid;
preferably, the operation of filtering by using a sterilizing filter membrane is also independently included after the anhydrous sodium sulfate is added to remove residual water.
10. Use of the composition with anti-dandruff and anti-itch effects of any one of claims 1-4 or the preparation method of any one of claims 5-9 in anti-dandruff, anti-itch, bacteriostatic daily care products.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199461A (en) * | 2006-12-12 | 2008-06-18 | 王洪飞 | Skin-protecting and hair-protecting products containing prinsepia utilis royle oil |
| US20120308637A1 (en) * | 2009-11-03 | 2012-12-06 | Guy Chamberland | Compositions comprising extracts of boswellia, tea tree, aloe and lavender oil and methods of treating wounds, burns and skin injuries therewith |
| CN104288003A (en) * | 2014-09-18 | 2015-01-21 | 青岛永通电梯工程有限公司 | Hair care essential oil with effects of relieving itching and removing dandruff |
| CN105997596A (en) * | 2016-07-08 | 2016-10-12 | 拉芳家化股份有限公司 | Dandruff removing composition containing plant essential oil having synergistic effect and application thereof |
| CN107468571A (en) * | 2017-08-25 | 2017-12-15 | 广西和桂集团有限公司 | A kind of shampoo containing Chinese herbal medicine and preparation method thereof |
-
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- 2023-03-01 CN CN202310185519.0A patent/CN115919696B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199461A (en) * | 2006-12-12 | 2008-06-18 | 王洪飞 | Skin-protecting and hair-protecting products containing prinsepia utilis royle oil |
| US20120308637A1 (en) * | 2009-11-03 | 2012-12-06 | Guy Chamberland | Compositions comprising extracts of boswellia, tea tree, aloe and lavender oil and methods of treating wounds, burns and skin injuries therewith |
| CN104288003A (en) * | 2014-09-18 | 2015-01-21 | 青岛永通电梯工程有限公司 | Hair care essential oil with effects of relieving itching and removing dandruff |
| CN105997596A (en) * | 2016-07-08 | 2016-10-12 | 拉芳家化股份有限公司 | Dandruff removing composition containing plant essential oil having synergistic effect and application thereof |
| CN107468571A (en) * | 2017-08-25 | 2017-12-15 | 广西和桂集团有限公司 | A kind of shampoo containing Chinese herbal medicine and preparation method thereof |
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