CN115885997B - Large-grain transverse ditch image pheromone attractant and application thereof - Google Patents
Large-grain transverse ditch image pheromone attractant and application thereof Download PDFInfo
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- CN115885997B CN115885997B CN202211693721.6A CN202211693721A CN115885997B CN 115885997 B CN115885997 B CN 115885997B CN 202211693721 A CN202211693721 A CN 202211693721A CN 115885997 B CN115885997 B CN 115885997B
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- 239000003016 pheromone Substances 0.000 title claims abstract description 42
- 239000005667 attractant Substances 0.000 title claims abstract description 36
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 36
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- 240000001717 Vaccinium macrocarpon Species 0.000 description 1
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- UAYKGOMDUQLCJS-UHFFFAOYSA-N ethylsulfanyl acetate Chemical compound CCSOC(C)=O UAYKGOMDUQLCJS-UHFFFAOYSA-N 0.000 description 1
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- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 description 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a large-grain transverse ditch elephant pheromone attractant and application thereof, belonging to the technical field of pheromone biological control. The macrogranulesten pheromone attractant comprises geraniol ethyl ester, cis-9-octadecenol and cis-2- (3, 3-dimethylcycloethyl) ethyl acetate. The large-grain transverse ditch image information attractant is added into a release carrier to prepare the large-grain transverse ditch image information attractant attracting core, so that a large amount of large-grain transverse ditch image adults can be attracted, and a green and safe biological prevention and control measure is provided for preventing and controlling the large-grain transverse ditch images.
Description
Technical Field
The application relates to the technical field of pest control, in particular to a large-grain transverse ditch elephant pheromone attractant and application thereof.
Background
Olive Olea europaea L is a famous subtropical tree and an important economic forest, and is mainly distributed in Gansu, guangdong, guangxi, yunnan, sichuan and other provinces in China. The olive oil contains rich nutrition and functional active ingredients, has the functions of preventing cardiovascular and cerebrovascular diseases, resisting tumors and the like, and has the reputation of vegetable oil queen. The large grain transverse ditch image Dyscerus cribripennis is the phloem and xylem of the pest olive, various woods and fruit trees, and is mainly used for eating the stem and pest trunk, branch and the like by adults and larvae, and damaging the guide tissue to cause necrosis of the branch, so that the plant is dried and dead. The large-grain cross-furrow elephant mostly uses adults in soil and larvae in bark to overwinter, and has the phenomenon of generation overlapping. The existing method for preventing and treating the large-grain transverse ditch image mainly comprises the steps of artificial physical and chemical pesticide prevention and treatment, and the artificial physical prevention and treatment are time-consuming and labor-consuming, and the large-area prevention and treatment are not practical. Chemical pesticide control is quick and efficient, but a large amount of pesticide residues can be increased after long-term use, the final product of the olive is edible olive oil, and the pesticide residues can influence the quality of the oil.
Along with the increase of the landscaping area of towns, the large-grain transverse ditch image is endangered from single host olive to multiple hosts. Large grain cross ditch damage occurs to the Fraxinus mandshurica in 2006, the Sichuan Cinnamomum camphora in 2010 and the Ningbo elephant mountain osmanthus fragrans in 2016.
The insect's own information chemical substance is used to control the harm of insect pest or to interfere with and prevent insect pest, so as to achieve the purpose of directly or indirectly controlling insect pest. The product has the advantages of environmental friendliness, no residue, safety and no toxicity to human and livestock, and has wide application prospect.
Insects of the family Amanidae are important pests of many agriculture and forestry, and about 27 species of Amanidae have been identified as pheromone components (Xiufang et al, forestry and environmental science 2019,35 (3): 107-115; yang Yi et al, university of Zhejiang agriculture and forestry, newspaper 2012, 29 (1): 5). The pheromones of most weevils are secreted by males and those of cotton boll weevils consist of (+) -cis-2-isopropenyl-1-methylcyclobutyl ethanol, cis-2- (3, 3-dimethyl-cyclohexylidene) acetaldehyde and trans-2- (3, 3-dimethyl-cyclohexylidene) acetaldehyde (hein P a et al Journal of Chemical Ecology,1979,5 (4): 617-627;MCKIBBN G H et al,Journal of Chemical Ecology,1977 (3): 331-335). The constituents of the walnut weevil and the cotton bollworm weevil are identical (HEDIN P A et al Journal of Chemical Ecology,1997, 23 (4): 965-978). The bark beetle, northern bark beetle and bark beetle pheromone are the same as each other, and are two substances, namely methanol and formaldehyde, but have different proportions (BOOTH D C et al Journal of Chemical Ecology,1983,9 (1): 1-12). The cranberry pheromone component cis-2- (3, 3-dimethyl-cyclohexylidene) ethanol, cis- (3, 3-dimethylcyclohexylidene) acetaldehyde, trans- (3, 3-dimethylcyclohexylidene) acetaldehyde is released by males, whereas geraniol females and males are both releasable (BIANCA G et al Journal of Chemical Ecology,2012, 38 (3): 272-277). However, the comprehensive control of the insects of the family Emamonaceae by utilizing pheromone is not applied much, and only the pheromone of the cotton boll weevil, the sweet potato weevil, the Asian nasal leucocoral weevil and the red palm weevil is used for pest monitoring and control, so that a certain control effect is achieved. According to the knowledge and verification of the prevention and treatment technology of the large-grain transverse ditch image, the large-grain transverse ditch image pheromone has not been identified, and no cases and records for preventing and treating the large-grain transverse ditch image by using the pheromone exist at home and abroad. Therefore, research and application of the large-grain transverse ditch pheromone product for prevention and control are of great significance.
Disclosure of Invention
The invention aims at providing a large-grain transverse ditch image pheromone attractant, a preparation method and application thereof.
The large-grain transverse ditch image pheromone component is identified by a gas chromatography and gas phase-antenna potential method.
The invention provides a large-grain transverse ditch elephant pheromone attractant, which comprises geraniol ethyl ester, cis-9-octadecenol and cis-2- (3, 3-dimethylethylene) ethyl acetate.
Preferably, the mass ratio of the geraniol ethyl ester, the cis-9-octadecenol and the cis-2- (3, 3-dimethylcycloethyl) ethyl acetate is 1:0.1 to 3:0.1 to 3.
Preferably, the mass ratio of the geraniol ethyl ester, the cis-9-octadecenol and the cis-2- (3, 3-dimethylcycloethyl) ethyl acetate is 1:2:2.
the invention also provides application of the large-grain transverse ditch image pheromone attractant in biological control of large-grain transverse ditch images. Specifically, the application is that geraniol ethyl ester, cis-9-octadecenol and cis-2- (3, 3-dimethylcycloethyl) ethyl acetate are added into a carrier to prepare the large-grain transverse ditch-like pheromone attractant core. The carrier is any one of a polyethylene pipe, a polyvinyl chloride pipe, a polyethylene bottle, a polyethylene slow-release bag, a polyvinyl chloride slow-release bag and a rubber plug. Preferably, the carrier is a polyethylene bottle.
When in use, the large-grain transverse ditch image information attractant is made into an attracting core, and the trapper with the large-grain transverse ditch image information attractant attracting core is hung in a hazard area of the large-grain transverse ditch image for monitoring and trapping the insect condition of the large-grain transverse ditch image in large quantity. The trap is a net-shaped trap. Preferably, the hanging height of the attractant is higher than the height of 1.5 m. Each of the lures contained 5000ug of the pheromone attractant, and the average spacing between the lures was 50m.
The beneficial effects of the invention include:
the large-grain transverse ditch image pheromone attractant provided by the invention can attract large-grain transverse ditch image adults in a large quantity, provides a nuisanceless pheromone prevention and control technology for preventing and controlling large-grain transverse ditch images, can monitor and trap large-grain transverse ditch image insects in a large quantity, has the advantages of environmental friendliness and no pollution, and is one of the technical keys for producing organic or green foods in economic forests such as olive.
Drawings
FIG. 1 is a GC-EAD electrophysiological response of large grain transverse furrow image adults to volatile organic compounds;
FIG. 2 is a graph of the ability of macrogranulesten pheromone attractant to trap male and female adults.
Detailed Description
The present invention is further illustrated and described below with reference to the following examples, which are but some, but not all, examples of the invention. All other inventions and embodiments, based on this invention and described herein, which would be apparent to one of ordinary skill in the art without undue burden are within the scope of this invention.
The experimental methods used in the following examples are conventional methods unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified. The synthesis of the pheromone component cis-2- (3, 3-dimethylcycloethyl) acetate was obtained by the synthesis method described in the publication "Kazuhiko Tanaka, et al," New Methods for Synthesis of a, β -Unsaturated Carboxylic Esters from Carbonyl Compounds Using Monoanions of Dithiocarbonates, and Dianions of Ethyl Mercaptoacetate and Ethyl 2-Mercaptopropionate ", bulletin of the Chemical Society of Japan,1979,52 (12), 3619-3625".
Example 1 identification of the large-grain transverse ditch image pheromone component
(1) Volatile organic component extraction
Sampling by a solvent extraction method: pulling out large transverse furrows like female and male adult hindgut with pointed tweezers, placing each 10 adult hindgut in a 1ml glass bottle, adding 100ul of n-hexane for extraction for 40min, concentrating n-hexane extract into 1ul, sealing in capillary glass tube, and preserving at-20deg.C for use. The male and female treatments were repeated 5 times.
(2) Identification of the Compound Components by GC-MS
The hindgut extract was analyzed by gas chromatography (Agilent 7890B-5977) and the column was DB-5MS
(30 m×0.25mm×0.25 μm), the carrier gas was high purity helium gas at a flow rate of 1ml/min, the sample was not split, and the split valve was opened after 1 min. Sample injection amount 1ul, sample inlet temperature 250 ℃, temperature raising program: the initial temperature is 80 ℃, the temperature is kept for 1min, the temperature is increased to 280 ℃ at 10 ℃/min, and the temperature is kept for 10min. MS analysis conditions: EI ion source, electron energy 70ev, transmission line temperature 250 ℃, ion source temperature 220 ℃, scanning range 41-650amu. Compound identification the structural identification of the compounds was carried out by comparing retention time and mass spectrum with standard compounds and with standard spectra in Nist02 spectra library, the results are shown in table 1 below.
TABLE 1 identification of volatile organics
Example 2 GC-EAD analysis of large grain transverse ditch image adults on volatile organic compounds
The above identified components were weighed 1000ug respectively, dissolved in 1ml of n-hexane to prepare a mixed solution with a concentration of 1000ug/ml, and then 25ul of the mixed solution was added to 475ul of n-hexane, diluted 20 times, and the concentration of each compound was 50ug/ml. And taking a large-grain transverse ditch image antenna, connecting two ends of the antenna with a glass electrode and a reference electrode which are provided with silver wires, and filling the glass electrode with insect physiological saline. The antenna was suspended 0.5cm from the orifice of an L-shaped glass tube in a continuous, moist air stream (air flow rate: 900 ml/min-1). The sample loading was 1ul. Using a B-5MS column, the temperature program for the GC was an initial temperature of 80℃for 1 minute, then 10℃per minute up to 280℃and 10 minutes. High purity helium was used as carrier gas at a flow rate of 20mL/min and post detector temperature of 300 ℃. The FID temperature was 300 ℃, helium was added to the diverter at 39mL/min and equally split between the Flame Ionization Detector (FID) and the EAD of the GC by a press-fit Y-separator (Agilent 7890). The GC effluent for the EAD was passed through a heater and mixed with humid air (300 mL/min,20 ℃). The EAD and FID signals obtained from the interface are displayed and analyzed by GC-EAD software. The mixtures were tested separately for large transverse furrows like four-headed males and four-headed females, each mixture was repeated 3 times, and the results are shown in fig. 1. As can be seen from FIG. 1, the large transverse furrows like female and male feelers are all for geraniol ethyl ester: cis-9-octadecenol: cis-2- (3, 3-dimethylethylene) ethyl acetate has obvious antennal potential reaction, which shows that the three components can be active components of the macropore transverse ditch image pheromone.
EXAMPLE 3 screening of the optimal formulation of the macrogranulesten attractant
The test is set in the olive industry park in the Sichuan Lizhou area, the average distance between the traps is 50m, the height of the trapping cores is set to be 1.5m from the ground, and the type of the traps is a net-shaped trap. Experiments were performed on the susceptibility design lures of male and female adults to volatile components, setting a total of 11 treatment groups and 1 blank group as shown in table 2 below, each treatment being repeated 3 times. The pheromone components were filled in polyethylene bottles (PE bottles, 15ml,Contech Enterprises,Delta,BC,Canada) according to a mass ratio, and each lure contained 5000ug of pheromone. The number and variety of trapped insects were collected and recorded once every 3 days for a continuous survey for 1 month. And (5) hanging the attractant at 9-11 am points when the weather is clear. When the attractant is hung, the sealing film is removed, the attractant is hung in the center of the trapper by using a thin iron wire, and trapping experimental data are recorded.
TABLE 2 Large grain transverse ditch image attractant treatment group trapping experimental data for different mass ratios of substances
The data in table 2 indicate that: (1) The trapping effect of the unit component pheromones (treatments 1-3) is general; (2) The trapping effect of the binary component pheromone (processing 4-6) is stronger than that of the unit component pheromone; (3) The ternary pheromone component ((treatments 7-11)) has the best trapping effect and is better than the formulas of the unit component and the binary component; the composite attractant prepared by the three components has the best attracting effect when the ratio of the three components is 1:2:2, and has important application value in the aspects of monitoring, preventing and controlling the large-grain transverse ditch image.
EXAMPLE 4 selection of optimal Release vector for pheromone attractant
The large-grain transverse ditch-like pheromone attractant prepared by the treatment group 10 in the example 3 is respectively prepared by adopting different slow release carriers including a rubber plug, a polyethylene bottle and a polyethylene slow release bag, respectively preparing lures according to the same method as the example 3, placing the lures into a trapping device with the same structure as the example 3 for test, setting the average distance between the traps to be 50m, setting the height of the lures to be 1.5m from the ground, setting the type of the traps to be a net-shaped trap, and repeating for 3 times. After the trap was set, the number of large transverse furrows trapped was investigated every 3 days, and the positions of the traps were randomly changed, and the investigation was continued for one month, with the results shown in table 3.
TABLE 3 trapping of large transverse ditch images by different release vectors
| Average trapping quantity (head/trap) | |
| Rubber plug | 33.67±0.57b |
| Polyethylene bottle | 56.33±0.32a |
| Polyethylene slow-release bag | 40.00±0.26b |
As can be seen from Table 3, the macrotransverse ditch-like pheromone attractant was best supported on polyethylene bottles.
Example 5 optimal suspension height screening of traps
The effect of different suspension heights on trapping effect was investigated using the macroreticular lateral furrows polyethylene bottle pheromone attractant in example 4.
The test method comprises the following steps: the lures were each prepared in the same manner as in treatment group 10 of example 3, placed in the same structure of trap apparatus as in example 3 for test, the average spacing between traps was 50m, the type of trap was a net-shaped trap, and 3 replicates were performed. After the trap was set, the number of large transverse furrows trapped was investigated every 3 days, and the positions of the traps were randomly changed, and the investigation was continued for one month, with the results shown in table 4.
TABLE 4 trapping of large transverse ditch images at different suspension heights of the trap
| Average trapping quantity (head/trap) | |
| 1m | 42±0.27b |
| 1.5m | 57.33±0.23ab |
| 2.0m | 63±0.43a |
As can be seen from Table 4, the trapping effect is better when the suspension height of the trap is higher than 1.5 m.
EXAMPLE 6 comparison of sex attractant Capacity of sex-male adults with large-grain transverse ditch image
The ability of the attractant to trap male and female adults was analyzed using the macrotransverse ditch image polyethylene bottle pheromone attractant of example 3.
The test method comprises the following steps: the lures were each prepared in the same manner as in the treatment group 10 of example 3, were put into a trap device of the same structure as in example 3 for test, the average spacing between the traps was 50m, the lure height was set to 1.5m from the ground, the trap type was a net-shaped trap, and 8 sets of traps were provided in total. After the trap was set, the number of the large transverse furrows trapped was investigated every 3 days, and the positions of the traps were randomly changed, and the investigation was continued for one month, and the results are shown in table 5 and fig. 2.
TABLE 5 statistics of sex attractant trapping of large-grain transverse ditch elephant female and male adults
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | Total number of | |
| Female adult | 43 | 28 | 15 | 24 | 40 | 33 | 41 | 35 | 259 |
| Male adult | 39 | 34 | 21 | 30 | 37 | 30 | 39 | 40 | 270 |
From the data analysis in Table 5 and FIG. 2, the large-grain transverse ditch image sex attractant has no significant difference (P is more than 0.05) on trapping of male and female adults, which indicates that the large-grain transverse ditch image sex attractant can simultaneously trap and kill male and female adults and can meet the large-grain transverse ditch image sex attractant for large-scale prevention and treatment.
Claims (8)
1. A macrogranulesten pheromone attractant, characterized in that the attractant comprises geraniol ethyl ester, cis-9-octadecenol and cis-2- (3, 3-dimethylcycloethyl) acetate;
the mass ratio of the geraniol ethyl ester to the cis-9-octadecenol to the cis-2- (3, 3-dimethylcycloethyl) ethyl acetate is 1:1 to 3:1 to 3.
2. An attractant according to claim 1, wherein the mass ratio of ethyl geraniol, cis-9-octadecenol, cis-2- (3, 3-dimethylcycloethyl) acetate is 1:2:2.
3. use of an pheromone attractant according to claim 1 or 2 for biological control of large transverse ditch images.
4. The use according to claim 3, wherein the use is to add geraniol ethyl ester, cis-9-octadecenol and cis-2- (3, 3-dimethylcycloethyl) acetate to a carrier to prepare a macrogranulesten pheromone attractant core.
5. The use according to claim 4, wherein the carrier is any one of a polyethylene tube, a polyvinyl chloride tube, a polyethylene bottle, a polyethylene slow release bag, a polyvinyl chloride slow release bag, and a rubber stopper.
6. The use according to claim 5, wherein the carrier is a polyethylene bottle.
7. The use according to claim 4, wherein the attractant is suspended at a height above 1.5m of the hazard zone of the macrogranulosa transverse ditch.
8. The use according to claim 4, wherein each of said lures comprises 5000ug of said pheromone attractant, and the average spacing between said lures is 50m.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6505434B1 (en) * | 1998-01-26 | 2003-01-14 | Lts Lohmann Therapie-Systeme Ag | Device for luring and catching insects |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6505434B1 (en) * | 1998-01-26 | 2003-01-14 | Lts Lohmann Therapie-Systeme Ag | Device for luring and catching insects |
Non-Patent Citations (2)
| Title |
|---|
| 大粒横沟象的生活习性及其防治;余光荣;四川林业科技(01);全文 * |
| 绿色威雷与噻虫啉防治油橄榄大粒横沟象试验;苏瑾;赵梦炯;刘鸿源;姜成英;芦娟;;甘肃林业科技(03);全文 * |
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