CN112889821B

CN112889821B - Sex pheromone composition of pinworm red bean and pink rice borer as well as lure and application thereof

Info

Publication number: CN112889821B
Application number: CN202110116611.2A
Authority: CN
Inventors: 黄敏佳; 张金桐; 宗世祥; 骆有庆; 黄建东
Original assignee: Beijing Changhui Linhai Ecological Technology Co ltd; Beijing Forestry University; Shanxi Agricultural University
Current assignee: Beijing Changhui Linhai Ecological Technology Co ltd; Beijing Forestry University; Shanxi Agricultural University
Priority date: 2021-01-28
Filing date: 2021-01-28
Publication date: 2021-12-14
Anticipated expiration: 2041-01-28
Also published as: CN112889821A

Abstract

The invention provides a sex pheromone composition for pine-stem-borer, and a lure and application thereof. The sex pheromone composition for pine-stem-borer comprises trans-11-hexadecenal, cis-11-hexadecene acetate and cis-3, cis-6, cis-9, cis-12 and cis-15-pentacarbon pentaene. The lure for the sex pheromone composition for pine-stem-borer is developed on the basis of the sex pheromone composition; the lure can effectively trap and kill male pine-stem-borer with a trap; the trap effect on the male pine-stem-borer is significantly higher than that of female pine-stem-borer (up to 3 times); and the lure can be applied to pest situation prediction and green prevention and control of pine-stem-borer, and avoids or reduces application of chemical pesticides, so pollution is reduced, the environment is protected, and significant economic, social and ecological comprehensive benefits are obtained.

Description

Sex pheromone composition of pinworm red bean and pink rice borer as well as lure and application thereof

Technical Field

The invention relates to the field of prevention and control of agricultural and forestry pests, and particularly relates to a sex pheromone composition of pinkeye of camphor trees, a lure and application thereof.

Background

The Latin literature name of the Pinus sylvestris is Diorcria mongolica Wang & Sung, and belongs to the genus Diaryotria Zeller (Diorcria) of the family Bombycidae (Pyralidae) of Lepidoptera (Lepidotera). China is mainly distributed in Heilongjiang, Jilin, inner Mongolia and other places. When a plurality of pine trees are damaged, larvae invade from wounds of branches and trunks, take phloem eating the trunks and boughs, the damaged parts are heavily fatted, and a coagulated fat mass mixed with tan insect feces is formed outside the wormhole. When seriously harmed, the coagulum can agglomerate into piles. After the pine tree is damaged, normal growth is influenced, and the trunk and the boughs are easy to break off from the damaged part or wither from the damaged part, so that withered tops and trunk shapes are seriously bent. Hosts of Pinus sylvestris L.var.mongolica, Pinus koraiensis (Pinus koraiensis) and grafted Pinus koraiensis with Pinus sylvestris as a rootstock and the like.

The larva of the pinworm pinkeye canker has the characteristic of being hidden as a pest, the larva is not easy to attract people's attention at the initial stage of infection, and the infection symptom can be considered as common wind break or wound fat flowing and is ignored. The pinworm eartip of the pinworm camphorata is a harmful pinworm camphorata and has the characteristic of low density, the pinworm camphorata is transferred into harmful substances, the diffusion speed is high, and once the forest stand is infected, the whole forest stand can be infected within 3-5 years generally. By the end of 2017 years, according to the survey and statistics of relevant departments, the emergence area of the pinkeye canker of the Pinus sylvestris in Heilongjiang province reaches more than 1.7 ten thousand hectares, more than 40 counties, regions or cities are covered, and the plant damage rate of individual forest stand reaches more than 90%. Although the pinkeye has a parasitic natural enemy, the natural control force is weaker.

The prior prevention and treatment technologies for the pinkeye of the camphor tree seeds mainly comprise three types: 1. and (3) manually removing the curdlan generated in the current year or cutting off the damaged lateral branches and intensively destroying the curdlan and the larvae and pupae in the galleries by combining forest tending. 2. The mixture of dichlorvos and diesel oil is applied on the fresh coagulated grease to kill the larvae in the galleries. 3. The prevention and treatment are carried out in a mode of injecting systemic medicament by punching a hole in a trunk. However, grease congeals and damaged lateral branches are manually removed, and the middle-upper part of the trunk is frequently removed; moreover, the wound caused at the joint of the lateral branch and the trunk is an important path for invasion of the pinkeye of the cinnamomum camphora. The point painting of the mixture of the dichlorvos and the diesel consumes manpower and has the condition of missing painting. In forests where the pinkeye moths in the camphor trees seriously happen, a large amount of diesel oil is used, and the hidden danger of forest fire exists. The trunk injection prevention and treatment cost is high, and the prevention and treatment effect is not obvious. In view of the serious threat of the pinkeye of the cinnamomum camphora in the northeast China to the healthy growth of the key tree species pinkeye of the sand fixation and forestation, the research and development of a technology for preventing and controlling the pinkeye of the cinnamomum camphora are urgently needed.

Disclosure of Invention

The invention aims to provide a sex pheromone composition of pinkeye of camphor trees, a lure and application thereof.

In order to achieve the object of the present invention, in a first aspect, the present invention provides a sex pheromone composition of pinkeye of cinnamomum camphora, which comprises trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosacene.

The mass ratio of trans-11-hexadecene aldehyde to cis-11-hexadecene acetate to cis-3, cis-6, cis-9, cis-12 and cis-15-pentacosatetraene in the composition is 1-5: 0.5-3: 0.5-2.

Preferably, the mass ratio of trans-11-hexadecene aldehyde to cis-11-hexadecene acetate to cis-3, cis-6, cis-9, cis-12, cis-15-pentacosapentenoic acid in the composition is 500:300: 200.

Further, the composition may also contain an antioxidant, such as 2, 6-di-tert-butyl-p-cresol.

In a second aspect, the invention provides a sex pheromone lure of the pinkeye of the cinnamomum camphora, which is made of rubber (such as organic silicon rubber), and a sex pheromone solution of the pinkeye of the cinnamomum camphora is added into the lure.

The Pink pin borer sex pheromone solution is prepared by taking the Pink pin borer sex pheromone composition as a solute and taking n-hexane, petroleum ether or dichloromethane as a solvent. Preferably, the solvent is n-hexane.

The concentration of the sex pheromone solution of the pinkeye of the camphor tree in the lure core is 0.001-0.01 mg/mu L.

In a third aspect, the invention provides a controlled release system for sex pheromone of the pinkeye of the cinnamomum camphorate of the pinkeye of the cinnamomum camphore of the pinkeye of the seed of the controlled release system of the controlled release of the pinkeye of the controlled release of the seed of the control release of the seed of the third aspect of the invention.

In a fourth aspect, the invention provides the application of the composition, the lure of the sex pheromone of the pinworm borer, or the controlled release system of the sex pheromone of the pinworm borer in trapping of the pinworm borer and the insect pest survey of the pinworm borer.

In a fifth aspect, the invention provides an application of the sex pheromone composition of the pinkeye, the sex pheromone lure core of the pinkeye, or the sex pheromone controlled release system of the pinkeye in preparation of the pinkeye attractant.

In the present invention,

the chemical structure of trans-11-hexadecenal is as follows:

the cis-11-hexadecene acetate has the following chemical structure:

the chemical structural formula of cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentaene is as follows:

the CAS numbers of trans-11-hexadecenal, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosatetraene are 57491-33-5, 34010-21-4 and 854201-96-0, respectively.

Compared with the prior art, the invention has at least the following advantages:

the invention discovers and identifies the sex pheromone of the pinworm borer on the pinworm tips of the cinnamomum camphora for the first time, which comprises trans-11-hexadecene aldehyde, cis-11-hexadecene acetate, cis-3, cis-6, cis-9, cis-12 and cis-15-pentacene, develops the sex pheromone luring core of the pinworm borer on the basis of the sex pheromone composition, can effectively lure and kill the male moths on the pinworm tips of the pinworm borer on the pinworm tips of the cinnamomum camphora by combining with a trapper, has the trapping effect on the male moths on the pinworm tips of the pinworm borer which is obviously higher than that of female moths (up to 3 times), can be applied to insect condition detection and green prevention and control of the pinworm borers on the pinworm tips of the pinworm borers of the cinnamomum camphora, avoids or reduces the application of chemical pesticides, thereby reducing pollution and protecting the environment, and has obvious economic, social and ecological comprehensive benefits.

Drawings

FIG. 1 is a total ion flow diagram and mass spectrum of sex pheromone extract of Pinctada sylvestris.

FIG. 2 is a gas chromatogram of the sex pheromone extract of Pinctada sylvestris (top) and the standard (bottom) of Pinctada sylvestris in the preferred embodiment of the present invention.

FIG. 3 is a forest test comparing moth induction levels for different combinations of compounds and different dosages in accordance with a preferred embodiment of the present invention. Different lower case letters indicate significant differences.

FIG. 4 shows the standard and the inspection results for differentiating the sex of the adult Pinctada camphorata.

Figure 5 shows a trap and rubber plug lure for forest testing in accordance with a preferred embodiment of the present invention.

FIG. 6 is a diagram of the adult Zhangzi Pinctada maxima trapped in the forest test in the preferred embodiment of the present invention.

FIG. 7 shows a method of installing traps in the pine forest land of Cinnamomum camphora according to a preferred embodiment of the present invention.

Fig. 8 shows the arrangement method (top) and moth-trapping effect (bottom) of the live female moth trap on the camphor pine forest land.

FIG. 9 is the chemical structural formula of sex pheromone of Dioscorea gynura.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.

The trans-11-hexadecenal, cis-11-hexadecene acetate, and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentenoic acid standards used in the following examples were purchased from Bedoukian Research Inc.

Example 1 extraction and identification of sex pheromone of Pinus sylvestris

1. Extraction of sex pheromones

Collecting a grease congee containing high-age larvae and pupae in a place where the zang-cinnamon twig borers seriously grow, putting the grease congee into a large net cage in a forest, and waiting for the larvae to pupate and eclose under natural environmental conditions. Adopting virgin female moth 2-3 days after eclosion, lightly pressing abdomen end with finger to extend sex pheromone gland in 5-6 hours of entering dark phase, then cutting off gland, rapidly placing into pointed test tube containing 1.0ml n-hexane, leaching for half an hour, concentrating with nitrogen gas to 20-50 μ l, and storing at-20 deg.C for use.

2. Analysis and identification of sex pheromones

(1) Analysis conditions of the gas chromatography-mass spectrometer are as follows: gas chromatograph Thermo scientific TRACETM1300, column DA-FFAP (30 m. times.0.25 mm. times.0.25 μm). The mass spectrometer is a Thermo scientific ISQTM single quadrupole GC-MS system, and the electron energy is 70 eV.

(2) Gas chromatography-flame ionization detector analysis conditions: gas chromatography Agilent 6890, column DB-1301(30 m.times.0.25 mm.times.0.25 μm).

The sex pheromone extract of the pinkeye of the cinnamomum camphora is analyzed by a gas chromatography-mass spectrometer (GC-MS), and the characteristic fragment ions of two substances in the extract are identified to be matched with the characteristic fragment ions of known compounds in a spectrum library through NIST17 spectrum library retrieval. Fig. 1 is a total ion flow diagram and a mass spectrum of a sex pheromone extract of pinkeye chufa, and the extract is preliminarily determined to contain two components, namely a component I: trans-11-hexadecenal and component II: cis-11-hexadecene acetate. And comparing whether the Retention Time (RT) of the sex pheromone extract of the pinkeye of the cinnamomum camphora with that of the standard substance by using a gas chromatography-flame ionization detector (GC-FID), and obtaining three components by comparing, wherein the three components comprise trans-11-hexadecene aldehyde (24.497min), cis-11-hexadecene acetate (27.627min) and cis 3, cis 6, cis 9, cis 12 and cis 15-pentacosaene (31.468min) in sequence according to the retention time. Fig. 2 is a gas chromatogram of sex pheromone extract of pinkeye of cinnamomum camphora and a standard substance, wherein the component I: trans-11-hexadecenal, component II: cis-11-hexadecene acetate, component III: cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentaene.

Example 2 preparation of sex pheromone lure of Pinctada sylvestris and forest moth-trapping test

Experimental example 1:

1. making lure (without antioxidant)

Rubber (figure 5) is adopted as a lure slow-release carrier, and a solvent is n-hexane. 100 mul of prepared sex pheromone composition solution of the pinkeye of the cinnamomum camphora is added into each lure.

2. Forest moth trapping test

The forest moth trapping test is carried out simultaneously in two places, namely a Xinjiang experimental forest land (A) and a Yun forest land (B) in the Qiqianhal city of Heilongjiang province, the test date is from the beginning of 7 months to the end of 7 months in 2020, the traps are triangular traps, each formula of the Pink pin moth sex pheromone composition is repeated for 6 times, the traps are hung at about 1.8 meters away from the ground of the Pink camphorata, the distance between the traps is about 40 meters, and the moth trapping amount is recorded every 3 days. The formula of the sex pheromone composition of the pinworm red bean is shown in the table 1:

TABLE 1

Note: e11-16: Ald, Z11-16: OAc and 25Hy respectively represent trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapene. A single newly emerged live female moth served as a control.

FIG. 3 shows the comparison of moth trapping amounts of different combinations of compounds and different dosages in forest tests (A: Xinjiang experimental forest farm; B: affluent forest farm). From the trapping data, the moth trapping amount of the attracting core containing trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosacene is obviously higher than that of the attracting core of other formulas and single-head live female moths no matter at the test point of the experimental forest farm in the New river or the test point of the forest farm in the affluence.

FIG. 4 shows the standard and the inspection results for differentiating the sex of the adult Pinctada camphorata. When the moth trapping amount is counted, the sex of adults on the armyworm plate is also synchronously examined, and the adults are male pink pine tip spotted borers (figure 4).

Experimental example 2:

1. making lure (with antioxidant)

Rubber is used as a lure core slow-release carrier, and a solvent is n-hexane. 100 mul of prepared sex pheromone composition solution of the pinkeye of the cinnamomum camphora is added into each lure.

A represents trans-11-hexadecene aldehyde, B represents cis-11-hexadecene acetate, C represents cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentaene, D represents solvent n-hexane, and E represents antioxidant 2, 6-di-tert-butyl-p-cresol.

Preparing a sex pheromone induction core of the pinkeye with the content of A being 500 mu g, the content of B being 300 mu g and the content of C being 200 mu g, wherein the solvent is n-hexane (D), the antioxidant is 2, 6-di-tert-butyl-p-cresol (E), and 3 groups of prepared sex pheromone induction cores are as follows:

in group 1, the weight parts of the sex pheromone composition D and E are 1:50:0.03

In group 2, the weight parts of the sex pheromone composition D and E are 1:50:0.04

In group 3, the weight parts of the sex pheromone composition D and E are 1:50:0.05

2. Forest moth trapping test

The results of the trapping test from 7 months 15 days to 7 months 25 days in 2020 are shown in Table 2:

TABLE 2

The adult Zhangzi Pinctada acutifolia (Roxb.) kurz trapped in forest test is shown in figure 6, and the method for arranging the trap in Pinus sylvestris forest is shown in figure 7. The trapper provided with the sex pheromone lure core of the pinkeye of the invention is hung on the big branch of the damaged pinkeye of the pinkeye. Each set of experiments was set to 3 replicates. Meanwhile, 1 trapper for hymen female moths of the pinkeye on camphor trees is arranged (figure 8), and the specific method is that the moths emerging on the same day are placed in a spherical metal net cage with the diameter of 6cm and then hung inside the trapper with a viscose plate, and the newly emerging moths are replaced every 2 days. The control is a blank lure to which only 50. mu.l of n-hexane was added. Counting the number of male moths on the sticky trap every 3 days and replacing the sticky trap in the trap.

The sex pheromone attracting core of the pinworm earworm of the invention has a significantly higher trapping effect on male moths of the pinworm earworm of the camphor, which can reach 3 times at most, and can be applied to the following control fields:

1) monitoring insect pest situation: the sex pheromone lure of the pinworm moth in the invention can be used for monitoring insect situations, and the occurrence and growth conditions of the pinworm moth in the forest can be known timely and accurately according to the trapping condition of male moths, thereby providing scientific basis for timely and effective comprehensive control.

2) Mass trapping: according to the insect situation monitoring result, the sex pheromone lure core of the pinworm earworm provided by the invention is hung in a damaged pine forest in the eclosion period of the pinworm earworm, so that a large amount of male moths can be continuously trapped, the mating chance of female moths is reduced, and the insect population density of offspring is greatly reduced, thereby achieving the purpose of reducing harm and being suitable for general popularization.

3) And (3) interfering mating: in the eclosion mating period of the pinkeye, sex pheromone lure cores provided by the invention are arranged among the damaged pine forests at a large density, so that the sex pheromone is diffused in the atmosphere of forest regions, the directional action of the sex pheromone released by female insects is disturbed, and male insects can not find the female insects and lose mating opportunities. Finally, the population quantity of pinus sylvestris offspring is reduced, and the damage degree of pinus sylvestris is reduced.

Example 3 orthogonal experiments

1. Making lure (without antioxidant)

2. Forest moth trapping test

The concentration gradient test of the Pinctada camphorata Pink-Shao pheromone composition is carried out in Xinjiang experimental forest farms in the Qizihaer city of Heilongjiang province, the test date is 7-15-7-25 days in 2020, the trap is a triangular trap, each formula of the Pinctada camphorata Pink-Shao pheromone composition is repeated for 6 times, and 50 mul of n-hexane is used as a blank control. The traps are hung at a place which is about 1.8 meters away from the ground, the distance between the traps is about 40 meters, and the moth trapping amount is recorded every 3 days. The concentration gradient test and moth-luring results of the sex pheromone composition of the pinworm earworm of the camphor tree are shown in the table 3:

TABLE 3 concentration gradient test (orthogonal design)

Note: e11-16: Ald, Z11-16: OAc and 25Hy respectively represent trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapene. 50 μ l n-hexane was used as a blank. Different lower case letters indicate significant differences (P < 0.01).

As can be seen from table 3, both the average moth-trapping amount and the total moth-trapping amount of formulation 9 were significantly higher than those of the other formulations. When the moth trapping amount is counted, the sex of adults on the armyworm plate is synchronously inspected, and the adults are male pink pine tip spotted borers. A single lure was charged with 500. mu.g of trans-11-hexadecenal and 300. mu.g of cis-11-hexadecene acetate as a preliminary formulation.

Example 4 Single factor investigation experiment

1. Making lure (without antioxidant)

2. Forest moth trapping test

The concentration gradient test of the sex pheromone composition of the pinkeye is carried out in a Xinjiang experimental forest farm in the Qizihaer city of Heilongjiang province, the test date is from 7 month 25 days to 7 month 30 days in 2020, the trap is a triangular trap, each formula of the sex pheromone composition of the pinkeye is repeated for 6 times, and 50 mul of n-hexane is used as a blank control. The traps are hung at a place which is about 1.8 meters away from the ground, the distance between the traps is about 40 meters, and the moth trapping amount is recorded every 3 days. The concentration gradient test and moth-luring results of the sex pheromone composition of the pinworm earworm of the camphor tree are shown in the table 4:

TABLE 4 Single factor investigation

Note: e11-16: Ald, Z11-16: OAc and 25Hy respectively represent trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentene. 50 μ l n-hexane was used as a blank. Different lower case letters indicate significant difference (P < 0.05).

As can be seen from table 4, both the average moth-trapping amount and the total moth-trapping amount of formulation 2 were significantly higher than those of the other formulations. When the moth trapping amount is counted, the sex of adults on the armyworm plate is synchronously inspected, and the adults are male pink pine tip spotted borers. A single lure was charged with 500. mu.g of trans-11-hexadecenal, 300. mu.g of cis-11-hexadecene acetate, and 200. mu.g of cis 3, cis 6, cis 9, cis 12, cis 15-pentacosatetraene as the final preferred formulation.

Example 5 species-specific investigation of Pinus sylvestris Dioryza punctiferalis sex pheromone composition

The known serious-damage yellow-eared rice borers in northeast China are mainly 6 types, namely, the pinkeye (D.mongoliella) pinkeye, the pinkeye (D.abietella), the pinkeye (D.sylvestrella), the pinkeye (D.mendacella), the pinkeye (D.pryeri) and the pinkeye (D.rubella). The sex attractant for the yellow tip borer was developed by woodmine et al in 1986, but there is no disclosure of the identification result of sex pheromone of the yellow tip borer. Before the present invention is disclosed, the sex pheromone of only fir tip spot borer and pine cone borer in the 6 kinds of tip spot borer has been reported publicly. There are 8 sex pheromones reported worldwide (table 5), and 6 sex pheromone components in total (fig. 9). The invention discloses and reports that trans-11-hexadecene aldehyde (E11-16: Ald), cis-11-hexadecene acetate (Z11-16: OAc) and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosacene are main components of sex pheromone of pinocembria cinnabaris. Among them, trans-11-hexadecenal is a new record of sex pheromone of female moth of Pinctada of the family Bombycidae.

As is clear from Table 5, the sex pheromone of the Selaginella fir (D.abietella) and the sex pheromone of the Dendrolimus punctatus (D.mendacella) were the same and both contained cis 9Trans 11-tetradecene acetate (Z9, E11-14: OAc) and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentenyl. Although the sex pheromone composition and the lure of the sex pheromone composition contain cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentaene, the sex pheromone composition does not contain cis 9, trans 11-tetradecene acetate. Furthermore, Strong et al (2008),

The others (2012) and the Hall et al (2017) respectively report that only cis 3, cis 6, cis 9, cis 12, cis 15-pentacosapentaene is added into the lure, and 3 pinkeye such as D.abitivorella, fir pinkeye and pinkeye can not be lured. Forest tests also show that the sex pheromone composition of the pinworm red cedar tip striped rice borer and the lure core thereof have no luring effect on the pinworm red cedar tip striped rice borer and the pinworm male moth. Furthermore, Hanula et al (1984) have reported that cis-11-hexadecene acetate (Z11-16: OAc) in the sex pheromone composition and the lure of the sex pheromone composition has obvious inhibition effect on male moths of the two American fruit tip ball borers, namely D.disclusa and D.clavaria.

The sex pheromone and the lure of the sex pheromone comprise trans-11-hexadecene aldehyde (E11-16: Ald) and cis-3, cis-6, cis-9, cis-12, cis-15-pentacosaene, and the sex attractant of the D.rubella (D.rubella) comprises cis-9, trans-11-tetradecene acetate and cis-11-hexadecene aldehyde (Z11-16: Ald). The sex pheromone of the pinkeye of the cinnamomum camphora and the lure thereof and the sex attractant of the pinkeye of the reddish tip are respectively provided with two compounds which are different from each other and one same compound. Woodmine et al (1986) reported that the sex attractant for the yellow tip borer (D.rubella) consisted of 3 compounds cis-11-hexadecene aldehyde (Z11-16: Ald), cis 9, trans 11-tetradecene acetate (Z9, E11-14: OAc) and cis-11-hexadecene acetate (Z11-16: OAc) in a ratio of 7:2: 1. The preferable proportion of trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosacene in the sex pheromone of the pinocembria camphorata walker and the lure core thereof is 5:3:2 (Table 5).

Female lepidopteran sex pheromones are typically aliphatic compounds. The chemical structures of female moth sex pheromone of insects of different families are differentiated in the aspects of carbon chain length, double bond number and position, terminal functional groups, cis-trans isomers and the like (figure 9); insects of different species in the same genus often have the same or similar sex pheromone but the ratio of the components varies from species to species (Table 5). The small differences in chemical structure and component ratios, which determine the specificity of insect species in chemical communication, are a result of the long-term evolution of insects in nature. Forest tests of three continuous years in Qizihaer city, Heilongjiang province show that the sex pheromone lure of the pinkeye mothball of the invention has no luring effect on other snout moth's moths of the pinkeye moths in northeast China. The results show that the sex pheromone composition of the pinkeye of the cinnamomum camphora has stronger species specificity.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Reference documents:

[1] wudelmin, Dingmeirong, Chi Junrong, Hades and clouds, 1986 research on sex attractant of the pink-tip striped rice borer, forestry science 22, 368-372, http:// www.linyekexue.net/CN/Y1986/V22/I4/368

[2]Strong,W.B.,Millar,J.G.,Grant,G.G.,Moreira,J.A.,Michael Chong,J.,Rudolph,C.,2008.Optimization of Pheromone Lure and Trap Design for Monitoring the Fir Coneworm,Dioryctria abietivorella.Entomol.Exp.Appl.126,67–77.https://doi.org/10.1111/j.1570-7458.2007.00635.x

[3]

C.,Svensson,G.P.,Jirle,E.V.,Rosenberg,O.,Roques,A.,Millar,J.G.,2012.(3Z,6Z,9Z,12Z,15Z)-Pentacosapentaene and(9Z,11E)-Tetradecadienyl Acetate:Sex Pheromone of the Spruce Coneworm Dioryctria abietella(Lepidoptera:Pyralidae).J.Appl.Entomol.136,70–78.https://doi.org/10.1111/j.1439-0418.2011.01619.x

[4]Hall,D.R.,Farman,D.,Domínguez,J.C.,Pajares,J.A.,2017.Female Sex Pheromone of the Cone Moth,Dioryctria mendacella:Investigation of Synergism between Type I and Type II Pheromone Components.J.Chem.Ecol.43,433–442.https://doi.org/10.1007/s10886-017-0846-8

[5]Hanula,J.L.,Berisford,C.W.,Debarr,G.L.,1984.Pheromone Cross-attraction and Inhibition Among Four Coneworms,Dioryctria spp.(Lepidoptera:Pyralidae)in a Loblolly Pine Seed Orchard.Environ.Entomol.13,1298–1301.https://doi.org/10.1093/ee/13.5.1298

Claims

1. The sex pheromone composition of the pinkeye is characterized by comprising trans-11-hexadecene aldehyde, cis-11-hexadecene acetate and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosaene;

wherein the mass ratio of the trans-11-hexadecene aldehyde to the cis-11-hexadecene acetate to the cis-3, cis-6, cis-9, cis-12 and cis-15-pentacosatetraene is 1-5: 0.5-3: 0.5-2.

2. The composition of claim 1, wherein the composition has a mass ratio of trans-11-hexadecenal, cis-11-hexadecene acetate, and cis 3, cis 6, cis 9, cis 12, cis 15-pentacosadecene of 500:300: 200.

3. The composition according to claim 1 or 2, characterized in that it further comprises an antioxidant.

4. The composition of claim 3, wherein the antioxidant is 2, 6-di-tert-butyl-p-cresol.

5. The sex pheromone lure core of the pinworm webworm is characterized in that the lure core is made of rubber, and a pinworm webworm sex pheromone solution is added into the lure core;

wherein, the sex pheromone solution of the Pink pin borer of the camphor tree is prepared by taking the composition of any one of claims 1 to 4 as a solute and n-hexane, petroleum ether or dichloromethane as a solvent.

6. The lure according to claim 5, wherein the concentration of the sex pheromone solution of the Pinctada camphorata in the lure is 0.001-0.01 mg/μ L.

7. The sex pheromone controlled release system of the pinkeye of the cinnamomum camphora is characterized by comprising a sex pheromone solution of the pinkeye of the cinnamomum camphora and a slow release carrier;

8. Use of the composition according to any one of claims 1 to 4, the lure according to claim 5 or 6, or the controlled release system according to claim 7 for trapping Pinctada camphorata and Pinctada camphorata insect reports.

9. Use of the composition according to any one of claims 1 to 4, the lure according to claim 5 or 6, or the controlled release system according to claim 7 for the preparation of an attractant for the pinkeye.