CN115869234A - Recombinant human collagen facial mask with repairing activity and preparation method thereof - Google Patents
Recombinant human collagen facial mask with repairing activity and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a recombinant human collagen mask with repairing activity and a preparation method thereof, wherein the mask comprises a close-contact mask carrier and a mask body, and the mask body comprises the following components in parts by weight: 3-10 parts of recombinant humanized collagen solution; 1-5 parts of a synergistic composition; 0.01-0.5 part of hydroxyethyl cellulose; 0.01-0.5 part of carbomer; 0.01-0.2 part of sodium methyl p-hydroxybenzoate; 0.01-0.2 part of sodium propyl p-hydroxybenzoate; 0.1-0.8 part of phenoxyethanol; 1-5 parts of glycerol; and (4) purifying the balance of water. In order to solve the problem of poor skin barrier repair effect in the prior art, the invention uses the effect promoting composition and the recombinant humanized collagen solution in combination, so that the moisture content of the stratum corneum of the skin is obviously improved, the reddening area a value, the transepidermal water loss value and the skin heme value of the skin are reduced, and the skin barrier repair, moisture retention and stability maintenance effects are achieved.
Description
Technical Field
The invention belongs to the technical field of mask preparation, and particularly relates to a recombinant human collagen mask with repairing activity and a preparation method thereof.
Background
The skin is the largest human organ, covers the surface of the human body, is directly contacted with the external environment, has important barrier protection effect, is structurally divided into epidermis, dermis, subcutaneous tissues and various skin appendages, is positioned at the outermost layer of the skin and has important skin barrier function; the skin barrier damage is mainly caused by facial skin diseases such as facial dermatitis eczema, rose acne and the like, repeated attack for a long time and unreasonable treatment are easy to cause skin sensitivity, the treatment difficulty of sensitive skin is high, the disease course is long, the skin barrier damage is caused by methods such as medicines, medical skin care products, photons and the like in clinic, the curative effect is not exact, and once the skin barrier damage is caused, the skin defense function is reduced, so that the problems of increase of percutaneous moisture loss, dry and sensitive skin, delayed wound healing, microbial imbalance, reduction of immune function, accelerated aging and the like are caused.
The recombinant human collagen is human collagen produced by fermentation, has a unique chemical structure, overcomes the inherent defects of animal collagen, has the advantages of low cost, no virus hidden trouble, good processability, good water solubility, low rejection reaction and the like, and has wide application prospects in the fields of biomedical materials, beauty cosmetics, food health care and the like, for example, chinese patent publication No. CN115400260A discloses a repair gel containing the recombinant human collagen and a preparation method thereof, and the repair gel comprises the following components in percentage by mass: 0.01-2% of efficacy substance, 0.5-8% of thickening agent, 2-10% of humectant, 0.1-1% of coupling agent, 0.01-1% of medical preservative and the balance of purified water, and the recombined human-derived collagen III and the recombined human-derived collagen IV are supplemented to promote skin cell regeneration and form an IV-type collagen protective film with a macromolecular reticular structure.
Disclosure of Invention
In view of the deficiencies in the prior art, the inventors of the present invention have made extensive studies and extensive practices to provide technical solutions of the present invention. The technical solution, its implementation and principles, etc. will be further explained as follows.
The technical scheme of the invention is as follows: the invention provides a recombinant human-derived collagen mask with repairing activity and a preparation method thereof.
In some embodiments of the present invention, the mask body comprises the following components in parts by weight: 3-10 parts of recombinant humanized collagen solution; 1-5 parts of a synergistic composition; 0.01-0.5 part of hydroxyethyl cellulose; 0.01-0.5 part of carbomer; 0.01-0.2 part of sodium methyl p-hydroxybenzoate; 0.01-0.2 part of sodium propyl p-hydroxybenzoate; 0.1-0.8 part of phenoxyethanol; 1-5 parts of glycerol; and (4) purifying the balance of water.
Preferably, the mask body comprises the following components in parts by weight: 5-10 parts of recombinant humanized collagen solution; 1-3 parts of an effect promoting composition; 0.05-0.25 part of hydroxyethyl cellulose; 0.05-0.15 part of carbomer; 0.03-0.13 part of sodium methyl p-hydroxybenzoate; 0.02-0.1 part of sodium propyl p-hydroxybenzoate; 0.2-0.5 part of phenoxyethanol; 3-5 parts of glycerol; the balance of purified water.
Preferably, the concentration of the contained recombinant humanized collagen solution is 1-15mg/mL.
Preferably, the close-fitting type mask carrier is selected from one of non-woven fabrics, silk or biological fibers.
In some embodiments of the invention, the synergistic composition comprises the following components in parts by weight: 1-5 parts of gamma-oryzanol; 0.1-0.8 part of cubilose acid; 0.1-2 parts of butterflybush flower extract.
Preferably, the promoting composition comprises the following components in parts by weight: 1-3 parts of gamma-oryzanol; 0.1-0.5 part of cubilose acid; 0.5-2 parts of butterflybush flower extract.
Preferably, the preparation method of the buddleja officinalis extract comprises the following steps: soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting under air pressure for 3-4h, wherein the weight ratio of the flos Buddlejae powder to lactic acid is 1.
Further, the preparation method of the recombinant humanized collagen solution comprises the following steps: preparing the recombinant human collagen into a solution of 10-12g/L, adjusting the pH to 8-9, and adding 0.1% by volume of protease and 0.1% by volume of trypsin for hydrolysis for 3 hours; and inactivating enzyme after the reaction is finished, centrifuging, collecting supernate, and freeze-drying to obtain the recombinant humanized collagen solution.
Preferably, the molecular weight of the recombinant humanized collagen solution is in the range of 204-2530Da.
In some embodiments of the present invention, the present invention also provides a preparation method of a recombinant human collagen mask having a repairing activity, the preparation method comprising the steps of:
(1) Weighing hydroxyethyl cellulose, carbomer, sodium methyl paraben, sodium propyl paraben, phenoxyethanol, glycerol and purified water, stirring and dissolving at 80 deg.C, sterilizing at 115 deg.C for 30min, and cooling to below 40 deg.C to obtain mixture A;
(2) Filtering the recombinant humanized collagen solution through a 0.22-micron filter membrane, adding the filtered recombinant humanized collagen solution into the mixture A obtained in the step (1), and stirring and mixing to obtain a mixture B;
(3) Adding the effect promoting composition into the mixture B, stirring and mixing to obtain the mask body, and filling the mask body into a sterile film bag product;
(4) And folding the close-fitting type mask carrier, soaking the close-fitting type mask carrier in 25g of mask body, and sealing to obtain the recombinant human collagen mask.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a recombinant human collagen facial mask with repairing activity and a preparation method thereof, after the recombinant human collagen facial mask is used, the moisture content of the stratum corneum of the skin is increased, the value of a red area a of the skin, the value of water loss through the epidermis and the value of skin heme of the skin are reduced, so that the recombinant human collagen facial mask can be used for repairing the skin barrier, increasing the moisture content of the skin, and relieving and improving the red state of the skin;
the recombinant human collagen type III human collagen sequence of the active component recombinant human collagen solution in each facial mask is a sequence which is screened and optimized for a long time, the preparation method is described in CN107033238B which is an authorization of Chinese patent and a purification method and a preparation method of the recombinant human collagen type III, and fermentation liquor which is obtained by fermenting recombinant yeast with the capacity of expressing the recombinant human collagen type III is subjected to heat treatment at the temperature of 65-75 ℃, so that the recombinant human collagen type III in the fermentation liquor is effectively prevented from being degraded into small molecular peptides, the production of endotoxin in the fermentation liquor is reduced, and the purity of the recombinant human collagen type III is greatly improved;
the recombinant human III-type collagen has strong water solubility and bioactivity, has hydrophilic amino acid residues, strong hydrophilicity and adhesiveness, and is favorable for adhesion growth of fibroblasts; a microenvironment is created for the cells in a short time, and the cells are guided to rapidly enter the damaged part to be repaired of the skin for growth, so that the skin barrier is repaired; the butterflybush flower extract in the efficacy promoting composition is rich in sodium hyaluronate and glucoside substances, and can promote the efficacy of a recombined humanized collagen solution when being used in combination with gamma-oryzanol and cubilose acid, so that the skin barrier repair is cooperatively performed, the moisture is effectively locked, and the synthesis of collagen of human skin is promoted;
in addition, the hydroxyethyl cellulose has good solubility and viscosity, can keep the original shape of the cosmetic in cold and hot alternate seasons, and has the characteristic of moisture retention; carbomer has good affinity to skin, is a common thickening agent in cosmetics and increases the viscosity of the cosmetics; the sodium methyl p-hydroxybenzoate and the sodium propyl p-hydroxybenzoate are common preservatives for cosmetics, have broad-spectrum balanced inhibiting effect on bacteria, saccharomycetes and mould, are typical antibacterial and antiseptic products, and have certain bactericidal capacity; the phenoxyethanol acts on cell membranes to increase the passing rate of potassium ions and reduce the activity of enzyme, thereby inhibiting the growth of cells, achieving the antibacterial effect and having the antiseptic effect;
meanwhile, the softest and exquisite silk is adopted as the clingy facial mask carrier, and the clingy facial mask can cling to the texture of the skin, so that the degree of fitting of the applied face is very high, the facial mask is thin, transparent and breathable, the corners of the nose, the wings and the mouth can be fitted, water is locked and is replenished for a long time, the silk facial mask can accelerate the repair of the skin, and the damaged skin can be repaired quickly through the exquisite fitting of silk fiber.
Drawings
FIG. 1 is a graph showing the measurement result of the moisture content of the stratum corneum after the recombinant human collagen facial mask prepared by the invention is used;
FIG. 2 is a diagram showing the result of measuring the water loss value of epidermis after the recombinant human collagen facial mask prepared by the invention is used;
FIG. 3 is a graph of the measurement result of the skin hemoglobin value of the recombinant human collagen facial mask prepared by the invention;
fig. 4 is a graph showing a measurement result of a value of a reddish area after the recombinant human collagen facial mask prepared by the present invention is used.
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Moreover, any methods and materials similar or equivalent to those described herein can be used in the practice of the present invention. The preferred embodiments and materials described herein are exemplary only, and are not intended to limit the scope of the present application.
The experimental methods in the following examples are all conventional methods unless otherwise specified; the test materials and test strains used in the following examples were all commercially available unless otherwise specified.
Example 1
Recombinant human collagen mask with repairing activity and preparation method thereof
The recombinant humanized collagen mask comprises a close-contact mask carrier and a mask body, and the mask body is prepared from the raw materials of a recombinant humanized collagen solution, hydroxyethyl cellulose, carbomer, a promoter composition, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water.
The mask body comprises the following components in parts by weight (g/part): 10 parts of recombinant humanized collagen solution; 1 part of a synergistic composition; 0.1 part of hydroxyethyl cellulose; 0.15 part of carbomer; 0.04 part of sodium methylparaben; 0.02 part of sodium propyl p-hydroxybenzoate; 0.3 part of phenoxyethanol; 3 parts of glycerol; 85.39 parts of purified water.
The concentration of the contained recombinant humanized collagen solution is 8mg/mL.
The close-fitting type mask carrier is one of non-woven fabrics.
The promoting composition comprises the following components in parts by weight: 1 part of gamma-oryzanol; 0.1 part of cubilose acid; and 0.5 part of butterflybush flower extract.
The preparation method of the buddleja officinalis extract comprises the following steps: soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting for 3 hours under air pressure, wherein the weight ratio of the butterflybush flower powder to the lactic acid is 1.
The preparation method of the recombinant humanized collagen solution comprises the following steps: preparing 10g/L solution of the recombinant human collagen, adjusting the pH to 8-9, and adding 0.1% by volume of protease and 0.1% by volume of trypsin for hydrolysis for 3h; and inactivating enzyme after the reaction is finished, centrifuging, collecting supernatant, and freeze-drying to obtain a recombinant humanized collagen solution, wherein the molecular weight range of the recombinant humanized collagen solution is 204-2530Da.
The invention also provides a preparation method of the recombinant human collagen facial mask with repairing activity, which comprises the following steps: (1) Weighing hydroxyethyl cellulose, carbomer, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water, stirring at 80 deg.C for dissolving, sterilizing at 115 deg.C for 30min, and cooling to below 40 deg.C to obtain mixture A; (2) Filtering the recombinant humanized collagen solution through a 0.22-micron filter membrane, adding the filtered recombinant humanized collagen solution into the mixture A obtained in the step (1), and stirring and mixing to obtain a mixture B; (3) Adding the effect promoting composition into the mixture B, stirring and mixing to obtain the mask body, and filling the mask body into a sterile film bag product; (4) And folding the close-contact type mask carrier, soaking the close-contact type mask carrier in 25g of mask material, and sealing to obtain the recombinant human collagen mask.
Example 2
Recombinant human collagen facial mask with repairing activity and preparation method thereof
The recombinant human collagen facial mask comprises a close-contact facial mask carrier and a facial mask body, and the raw materials for preparing the facial mask body comprise a recombinant human collagen solution, hydroxyethyl cellulose, carbomer, an effect promoting composition, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water.
The mask body comprises the following components in parts by weight (g/part): 5 parts of recombinant humanized collagen solution; 3 parts of an effect promoting composition; 0.2 part of hydroxyethyl cellulose; 0.1 part of carbomer; 0.1 part of sodium methyl p-hydroxybenzoate; 0.02 part of sodium propyl p-hydroxybenzoate; 0.2 part of phenoxyethanol; 5 parts of glycerol; 86.38 parts of purified water.
The concentration of the contained recombinant humanized collagen solution is 15mg/mL.
The close-fitting type mask carrier is selected from one of silk.
The promoting composition comprises the following components in parts by weight: 3 parts of gamma-oryzanol; 0.5 part of cubilose acid; and 2 parts of butterflybush flower extract.
Soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting for 4h under air pressure, wherein the weight ratio of the butterflybush flower powder to the lactic acid is 1.
The preparation method of the recombinant humanized collagen solution comprises the following steps: preparing the recombinant human collagen into a solution of 12g/L, adjusting the pH to 8-9, and adding protease with the volume ratio of 0.1% and trypsin with the volume ratio of 0.1% for hydrolysis for 3 hours; inactivating enzyme after the reaction is finished, centrifuging, collecting supernate, and freeze-drying to obtain a recombinant humanized collagen solution, wherein the molecular weight range of the recombinant humanized collagen solution is 204-2530Da.
The invention also provides a preparation method of the recombinant human collagen facial mask with repairing activity, which is carried out according to the embodiment 1.
Example 3
Recombinant human collagen facial mask with repairing activity and preparation method thereof
The recombinant humanized collagen mask comprises a close-contact mask carrier and a mask body, and the mask body is prepared from the raw materials of a recombinant humanized collagen solution, hydroxyethyl cellulose, carbomer, a promoter composition, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water.
The mask body comprises the following components in parts by weight (g/part): 8 parts of recombinant humanized collagen solution; 2 parts of a synergistic composition; 0.2 part of hydroxyethyl cellulose; 0.05 part of carbomer; 0.1 part of sodium methyl p-hydroxybenzoate; 0.1 part of sodium propyl p-hydroxybenzoate; 0.3 part of phenoxyethanol; 5 parts of glycerol; 84.25 parts of purified water.
The concentration of the contained recombinant humanized collagen solution is 1mg/mL.
The close-fitting mask carrier is selected from biological fibers.
The effect promoting composition comprises the following components in parts by weight: 2 parts of gamma-oryzanol; 0.3 part of cubilose acid; 1.2 parts of butterflybush flower extract.
Soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting for 3h under air pressure, wherein the weight ratio of the powder of the buddleja officinalis to the lactic acid is 1.
The preparation method of the recombinant humanized collagen solution comprises the following steps: preparing the recombinant human collagen into a solution of 10g/L, adjusting the pH to 8-9, and adding 0.1% by volume of protease and 0.1% by volume of trypsin for hydrolysis for 3 hours; and inactivating enzyme after the reaction is finished, centrifuging, collecting supernatant, and freeze-drying to obtain a recombinant humanized collagen solution, wherein the molecular weight range of the recombinant humanized collagen solution is 204-2530Da.
The invention also provides a preparation method of the recombinant human collagen facial mask with repairing activity, which is carried out according to the embodiment 1.
Example 4
Recombinant human collagen mask with repairing activity and preparation method thereof
The recombinant human collagen facial mask comprises a close-contact facial mask carrier and a facial mask body, and the raw materials for preparing the facial mask body comprise a recombinant human collagen solution, hydroxyethyl cellulose, carbomer, an effect promoting composition, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water.
The mask body comprises the following components in parts by weight (g/part): 10 parts of recombinant humanized collagen solution; 1 part of an effect promoting composition; 0.25 part of hydroxyethyl cellulose; 0.1 part of carbomer; 0.13 part of sodium methyl p-hydroxybenzoate; 0.02 part of sodium propyl p-hydroxybenzoate; 0.2 part of phenoxyethanol; 4 parts of glycerol; 84.3 parts of purified water.
The concentration of the contained recombinant humanized collagen solution is 5mg/mL.
The close-fitting type mask carrier is selected from silk.
The promoting composition comprises the following components in parts by weight: 3 parts of gamma-oryzanol; 0.3 part of cubilose acid; 1 part of butterflybush flower extract.
Soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting for 3h under air pressure, wherein the weight ratio of the powder of the buddleja officinalis to the lactic acid is 1.
The preparation method of the recombinant humanized collagen solution comprises the following steps: preparing 10g/L solution of the recombinant human collagen, adjusting the pH to 8-9, and adding 0.1% by volume of protease and 0.1% by volume of trypsin for hydrolysis for 3h; inactivating enzyme after the reaction is finished, centrifuging, collecting supernate, and freeze-drying to obtain a recombinant humanized collagen solution, wherein the molecular weight range of the recombinant humanized collagen solution is 204-2530Da.
The invention also provides a preparation method of the recombinant human collagen facial mask with repairing activity, which is carried out according to the embodiment 1.
Example 5
Recombinant human collagen facial mask with repairing activity and preparation method thereof
The recombinant human collagen facial mask comprises a close-contact facial mask carrier and a facial mask body, and the raw materials for preparing the facial mask body comprise a recombinant human collagen solution, hydroxyethyl cellulose, carbomer, an effect promoting composition, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water.
The mask body comprises the following components in parts by weight (g/part): 5 parts of recombinant humanized collagen solution; 2 parts of a synergistic composition; 0.05 part of hydroxyethyl cellulose; 0.15 part of carbomer; 0.03 part of sodium methylparaben; 0.1 part of sodium propyl p-hydroxybenzoate; 0.5 part of phenoxyethanol; 3 parts of glycerol; 89.17 parts of purified water.
The concentration of the contained recombinant humanized collagen solution is 10mg/mL.
The close-fitting facial mask carrier is selected from silk.
The promoting composition comprises the following components in parts by weight: 2 parts of gamma-oryzanol; 0.3 part of cubilose acid; 1 part of butterflybush flower extract.
Soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting for 3h under air pressure, wherein the weight ratio of the powder of the buddleja officinalis to the lactic acid is 1.
The preparation method of the recombinant humanized collagen solution comprises the following steps: preparing 10g/L solution of the recombinant human collagen, adjusting the pH to 8-9, and adding 0.1% by volume of protease and 0.1% by volume of trypsin for hydrolysis for 3h; and inactivating enzyme after the reaction is finished, centrifuging, collecting supernatant, and freeze-drying to obtain a recombinant humanized collagen solution, wherein the molecular weight range of the recombinant humanized collagen solution is 204-2530Da.
The invention also provides a preparation method of the recombinant human collagen facial mask with repairing activity, which is carried out according to the embodiment 1.
Comparative example 1
The comparative example provides a recombinant human collagen facial mask with repairing activity and a preparation method thereof, which are different from the facial mask in example 4 only in that all components do not contain the effect promoting composition, and the rest components and the content of the components are the same as those in example 4.
Comparative example 2
The comparative example provides a recombinant humanized collagen mask with repairing activity and a preparation method thereof, and the facial mask is different from the facial mask in example 4 only in that all components do not contain a recombinant humanized collagen solution, and the rest components and the component content are the same as those in example 4.
Performance testing
In order to verify the repairing performance of the recombinant human collagen facial mask prepared by the invention, the recombinant human collagen facial masks prepared in example 4, comparative example 1 and comparative example 2 are sent to Hangzhou Saiviu quality technical service company Limited for testing.
The test method and the process are as follows: recruiting qualified subjects, under the guidance of detection personnel, cleaning the faces of the subjects by using a laboratory facial cleaning product, washing the faces with clear water, then sucking the faces with a non-scrap water-absorbing dry paper towel, sitting still for 20 minutes in a rest waiting area with the temperature of 21 +/-1 ℃ and the relative humidity of 50 +/-10%, exposing and keeping the faces relaxed, and measuring the numerical values before use by using the method shown in the table 1; the test subjects used the recombinant human collagen facial mask prepared by the invention, and the water loss value of the facial skin of the test subjects after use, the water content of the skin stratum corneum, the skin heme value and the reddening area a after 14 and 28 days of the use are measured * Values, data recorded before, 14 days, 28 days of use, test environment: temperature: 20-22 ℃, relative humidity: 40% -60%, and the test indexes and instruments are shown in table 1.
Inclusion criteria were: 1) Healthy subjects between 18 and 60 years of age (except pregnant or lactating women); 2) Transepidermal water loss value of cheekbones>15g/h/m 2 A step of; 3) The skin of the face is easy to be dry, itching, reddish, fragile, sensitive and rough; 4) The facial skin has no obvious skin damage, scar and the like(ii) a 5) The test can be well matched according to the requirements of the scheme, and the regularity of life can be kept during the test; 6) Voluntarily participate in the trial, being able to read and understand all the contents of the informed consent and sign the informed consent.
Exclusion criteria: 1) Those who use antihistamines for nearly one week or immunosuppressants for nearly one month; 2) Patients with skin diseases such as psoriasis, eczema, atopic dermatitis, severe acne, etc., or other judgers which may affect the experimental results; 3) Allergic diseases, people with allergy history of cosmetics or other external preparations; 4) Patients who have undergone facial cosmetic surgery, other cosmetic modes or external medicine within nearly three months; 5) Insulin dependent diabetes mellitus patients; 6) Patients suffering from asthma or other chronic respiratory diseases undergoing treatment; 7) Those who receive anti-cancer chemotherapy within approximately six months; 8) Patients with immunodeficiency or an autoimmune disease; 9) Lactating or pregnant women, or those with a pregnancy plan in the near future; 10 Face attending other clinical trials in or near bimonths; 11 Those with high constitutional sensitivity; 12 Those who cannot complete the specified content according to the test requirements; 13 In addition to the above, the worker judges it is not suitable for the efficacy tester.
TABLE 1 skin repair experiment test indexes
The data statistical analysis method comprises the following steps: performing descriptive statistics on the measured values of all the test areas by using SPSS software; the data improvement value normal distribution significance Test is carried out by Shapiro-Wilk Test, which indicates that the significance difference level reaches 0.05, and the difference is significant; * Indicates that the level of significant difference reaches 0.01, and the difference is extremely significant.
As shown in fig. 1, the moisture content of the stratum corneum of the skin is significantly increased after the recombinant human collagen facial mask of example 4 is used, the barrier function of the skin becomes good, while the difference of comparative example 2 is not obvious, which indicates that the recombinant human collagen solution can adjust the moisture content of the skin, and compared with comparative example 1, the effect of example 4 is better, which indicates that the effect promoting composition has the effect of promoting the recombinant human collagen solution to exert the effect, and similarly, as shown in fig. 2, fig. 3 and fig. 4, the redness area a value, the loss value of the moisture through the epidermis and the skin hemoglobin value of the skin can be reduced after the recombinant human collagen facial mask of example 4 is used, and in conclusion, the recombinant human collagen facial mask prepared by the invention can repair and care the barrier of the skin, increase the moisture content of the skin, and reduce and improve the redness state of the skin.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
The present invention and its embodiments have been described above, and the description is not intended to be limiting, and what is shown in the drawings is only one embodiment of the present invention, and the practical application is not limited thereto. In summary, those skilled in the art should be able to devise similar arrangements and embodiments without the inventive intent without departing from the spirit and scope of the present invention.
Claims (10)
1. A recombinant human collagen facial mask with repairing activity is characterized in that: the recombinant human-derived collagen mask comprises a close-contact mask carrier and a mask body, wherein the mask body comprises the following components in parts by weight: 3-10 parts of recombinant humanized collagen solution; 1-5 parts of a synergistic composition; 0.01-0.5 part of hydroxyethyl cellulose; 0.01-0.5 part of carbomer; 0.01-0.2 part of sodium methyl p-hydroxybenzoate; 0.01-0.2 part of sodium propyl p-hydroxybenzoate; 0.1-0.8 part of phenoxyethanol; 1-5 parts of glycerol; and (4) purifying the balance of water.
2. The recombinant human collagen facial mask with repairing activity according to claim 1, wherein: the mask body comprises the following components in parts by weight: 5-10 parts of recombinant humanized collagen solution; 1-3 parts of an effect promoting composition; 0.05-0.25 part of hydroxyethyl cellulose; 0.05-0.15 part of carbomer; 0.03-0.13 part of sodium methyl p-hydroxybenzoate; 0.02-0.1 part of sodium propyl p-hydroxybenzoate; 0.2-0.5 part of phenoxyethanol; 3-5 parts of glycerol; and (4) purifying the balance of water.
3. The recombinant human collagen facial mask with repairing activity as claimed in claim 2, wherein: the concentration of the contained recombinant humanized collagen solution is 1-15mg/mL.
4. The recombinant human collagen facial mask with repairing activity as claimed in claim 3, wherein: the close-fitting type mask carrier is selected from one of non-woven fabrics, silk and biological fibers.
5. The recombinant human collagen facial mask with repairing activity according to claim 4, wherein: the promoting composition comprises the following components in parts by weight: 1-5 parts of gamma-oryzanol; 0.1-0.8 part of cubilose acid; 0.1-2 parts of butterflybush flower extract.
6. The recombinant human collagen facial mask with repairing activity as claimed in claim 5, wherein: the promoting composition comprises the following components in parts by weight: 1-3 parts of gamma-oryzanol; 0.1-0.5 part of cubilose acid; 0.5-2 parts of butterflybush flower extract.
7. The recombinant human collagen facial mask with repairing activity according to claim 6, wherein: the preparation method of the buddleja officinalis extract comprises the following steps: soaking the powder of the buddleja officinalis in water according to the weight ratio of 1 2 Extracting for 3-4 hours under air pressure, wherein the weight ratio of the butterflybush flower powder to the lactic acid is 1.
8. The recombinant human collagen facial mask with repairing activity according to claim 7, wherein: the preparation method of the recombinant humanized collagen solution comprises the following steps: preparing 10-12g/L solution of the recombinant human collagen, adjusting the pH to 8-9, and adding 0.1% by volume of protease and 0.1% by volume of trypsin for hydrolysis for 3h; and inactivating enzyme after the reaction is finished, centrifuging, collecting supernatant, and freeze-drying to obtain the recombinant humanized collagen solution.
9. The recombinant human collagen facial mask with repairing activity according to claim 8, wherein: the molecular weight range of the recombinant humanized collagen solution is 204-2530Da.
10. The preparation method of the recombinant human collagen facial mask with repairing activity as claimed in claim 9, wherein: the preparation method comprises the following steps:
(1) Weighing hydroxyethyl cellulose, carbomer, sodium methyl p-hydroxybenzoate, sodium propyl p-hydroxybenzoate, phenoxyethanol, glycerol and purified water, stirring at 80 deg.C for dissolving, sterilizing at 115 deg.C for 30min, and cooling to below 40 deg.C to obtain mixture A;
(2) Filtering the recombinant humanized collagen solution through a 0.22-micron filter membrane, adding the filtered recombinant humanized collagen solution into the mixture A obtained in the step (1), and stirring and mixing to obtain a mixture B;
(3) Adding the effect promoting composition into the mixture B, stirring and mixing to obtain the mask body, and filling the mask body into a sterile film bag product;
(4) And folding the close-fitting type mask carrier, soaking the close-fitting type mask carrier in 25g of mask body, and sealing to obtain the recombinant human collagen mask.
Priority Applications (1)
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| EP1165035A2 (en) * | 1999-03-26 | 2002-01-02 | Parfums Christian Dior | Cosmetic or dermatological compositions containing at least one substance for increasing the functionality and/or expression of cd44 membrane receptors of skin cells |
| CN110772444A (en) * | 2019-12-03 | 2020-02-11 | 广州留今科学研究有限公司 | Skin conditioner and mask for whitening and removing wrinkles and preparation method thereof |
| CN112043643A (en) * | 2020-09-29 | 2020-12-08 | 代晓娟 | Preparation method of natural anti-aging moisturizing mask based on human collagen |
| CN112891258A (en) * | 2021-02-03 | 2021-06-04 | 广州市科能化妆品科研有限公司 | Natural sun protection element and preparation method and application thereof |
| CN114159335A (en) * | 2021-12-13 | 2022-03-11 | 武汉百思凯瑞生物科技有限公司 | Cubilose acid moisturizing and repairing nano composition, preparation method thereof and cosmetic |
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|---|---|---|---|---|
| EP1165035A2 (en) * | 1999-03-26 | 2002-01-02 | Parfums Christian Dior | Cosmetic or dermatological compositions containing at least one substance for increasing the functionality and/or expression of cd44 membrane receptors of skin cells |
| CN110772444A (en) * | 2019-12-03 | 2020-02-11 | 广州留今科学研究有限公司 | Skin conditioner and mask for whitening and removing wrinkles and preparation method thereof |
| CN112043643A (en) * | 2020-09-29 | 2020-12-08 | 代晓娟 | Preparation method of natural anti-aging moisturizing mask based on human collagen |
| CN112891258A (en) * | 2021-02-03 | 2021-06-04 | 广州市科能化妆品科研有限公司 | Natural sun protection element and preparation method and application thereof |
| CN114159335A (en) * | 2021-12-13 | 2022-03-11 | 武汉百思凯瑞生物科技有限公司 | Cubilose acid moisturizing and repairing nano composition, preparation method thereof and cosmetic |
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