CN115867651A - 葡糖淀粉酶及其使用方法 - Google Patents

葡糖淀粉酶及其使用方法 Download PDF

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CN115867651A
CN115867651A CN202180043425.9A CN202180043425A CN115867651A CN 115867651 A CN115867651 A CN 115867651A CN 202180043425 A CN202180043425 A CN 202180043425A CN 115867651 A CN115867651 A CN 115867651A
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唐忠美
张正宏
葛晶
L·巴贝
奚兴祥
郝贺龙
C·黄
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Danisco US Inc
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Abstract

描述了重组宿主细胞、包含葡糖淀粉酶的组合物和使用葡糖淀粉酶糖化淀粉底物的方法。此外，本公开还涉及生产发酵产物的工艺和用于提高在动物中的淀粉消化率的方法以及生产发酵饮料的方法。该葡糖淀粉酶或1,4‑α‑D‑葡聚糖葡糖水解酶(EC 3.2.1.3)优选地来自毛霉菌目进化枝，特别地与来自瓶霉的葡糖淀粉酶具有高序列同一性。

Description

葡糖淀粉酶及其使用方法
相关申请的交叉引用
本申请要求2020年4月17日提交的国际专利申请号PCT/CN 2020/085393的优先权，该国际专利申请的公开内容通过引用以其全文并入本文。
技术领域
本公开涉及重组宿主细胞、包含葡糖淀粉酶的组合物和使用葡糖淀粉酶糖化淀粉底物的方法。此外，本公开还涉及生产发酵产物的工艺和用于提高在动物中的淀粉消化率的方法以及生产发酵饮料的方法。
背景技术
葡糖淀粉酶(1,4-α-D-葡聚糖葡糖水解酶，EC 3.2.1.3)是催化从淀粉或相关寡糖和多糖分子的非还原端释放D-葡萄糖的酶。葡糖淀粉酶由几种丝状真菌和酵母产生。
葡糖淀粉酶的主要应用是将部分加工的淀粉/糊精糖化成葡萄糖，葡萄糖是许多发酵工艺的必需底物。然后可以使用发酵生物将葡萄糖直接或间接地转化成发酵产物。商业发酵产物的实例包括醇类(例如乙醇、甲醇、丁醇、1,3-丙二醇)；有机酸(例如柠檬酸、乙酸、衣康酸、乳酸、葡糖酸、葡糖酸盐、乳酸、琥珀酸、2,5-二酮基-D-葡糖酸)；酮(例如丙酮)；氨基酸(例如谷氨酸)；气体(例如H2和CO2)以及更复杂的化合物。
终产物还可以是糖浆。例如，终产物可以是葡萄糖，但还可以(例如通过葡萄糖异构酶)转化成果糖或由几乎相等地葡萄糖和果糖构成的混合物。这种混合物，或进一步富集果糖的混合物，是在世界范围内商业上最常用的高果糖玉米糖浆(HFCS)。
尽管据报道，多种微生物产生葡糖淀粉酶(因为它们在细胞外分泌大量的酶)，但用于商业目的的葡糖淀粉酶传统上使用丝状真菌生产。然而，商业上使用的真菌葡糖淀粉酶具有某些限制因素，如缓慢的催化活性或缺乏稳定性，这增加了工艺成本。
仍然需要新的葡糖淀粉酶以提高糖化效率并且提供高产发酵产物。
发明内容
本公开涉及重组宿主细胞、包含葡糖淀粉酶的组合物和使用葡糖淀粉酶糖化淀粉底物的方法。此外，本公开还涉及生产发酵产物的工艺和用于提高在动物中的淀粉消化率的方法以及生产发酵饮料的方法。
1.在一个方面，一种用于糖化淀粉底物的方法，所述方法包括将所述淀粉底物与葡糖淀粉酶接触，所述葡糖淀粉酶选自由以下组成的组：
(a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
(b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
(c)包含一种或多种序列基序的多肽，所述序列基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
2.在如段落1所述的方法的一些实施例中，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
3.在如段落2所述的方法的一些实施例中，其中所述多肽包含取代，该取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
4.在如段落1-3中任一项所述的方法的一些实施例中，所述多肽在对应于SEQ IDNO:61的多肽的位置66的位置处包含取代、缺失或添加。
5.在如段落4所述的方法的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
6.在如段落1-5中任一项所述的方法的一些实施例中，其中所述多肽包含SEQ IDNO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ IDNO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ IDNO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ IDNO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ IDNO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ IDNO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ IDNO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
7.在如段落1-6中任一项所述的方法的一些实施例中，其中所述淀粉底物是约15％至65％、15％至60％或15％至35％的干固体(DS)。
8.在如段落1-7中任一项所述的方法的一些实施例中，其中所述淀粉底物包含液化淀粉、糊化淀粉或颗粒状淀粉。
9.在如段落1-8中任一项所述的方法的一些实施例中，所述方法进一步包括向所述淀粉底物中添加己糖激酶、木聚糖酶、葡萄糖异构酶、木糖异构酶、磷酸酶、植酸酶、支链淀粉酶、β-淀粉酶、α-淀粉酶、蛋白酶、纤维素酶、半纤维素酶、脂肪酶、角质酶、海藻糖酶、异淀粉酶、氧化还原酶、酯酶、转移酶、果胶酶、水解酶、α-葡糖苷酶、β-葡糖苷酶或其组合。
10.在如段落1-9中任一项所述的方法的一些实施例中，其中糖化所述淀粉底物产生高葡萄糖糖浆，所述高葡萄糖糖浆包含一定量的选自由以下组成的组的葡萄糖：至少95.5％葡萄糖、至少95.6％葡萄糖、至少95.7％葡萄糖、至少95.8％葡萄糖、至少95.9％葡萄糖、至少96％葡萄糖、至少96.1％葡萄糖、至少96.2％葡萄糖、至少96.3％葡萄糖、至少96.4％葡萄糖、至少96.5％葡萄糖和至少97％葡萄糖。
11.在如段落1-10中任一项所述的方法的一些实施例中，所述方法进一步包括将所述高葡萄糖糖浆发酵成终产物。
12.在如段落11所述的方法的一些实施例中，其中糖化和发酵作为同时糖化和发酵(SSF)工艺进行。
13.在如段落11或12所述的方法的一些实施例中，其中所述终产物是醇，任选地乙醇。
14.在如段落11或12所述的方法的一些实施例中，其中所述终产物是选自由以下组成的组的生物化学品：氨基酸、有机酸、柠檬酸、乳酸、琥珀酸、谷氨酸一钠、葡糖酸、葡萄糖酸钠、葡萄糖酸钙、葡萄糖酸钾、葡萄糖酸δ-内酯、异抗坏血酸钠、ω3脂肪酸、丁醇、赖氨酸、衣康酸、1,3-丙二醇、生物柴油和异戊二烯。
15.在另一个方面，一种由淀粉底物生产发酵产物的工艺，所述工艺包括以下步骤：
1)液化所述淀粉底物；
2)糖化所述液化的淀粉底物；以及
3)用发酵生物发酵；
其中至少使用选自由以下组成的组的葡糖淀粉酶进行步骤2)：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
16.在如段落15所述的工艺的一些实施例中，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
17.在如段落16所述的工艺的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
18.在如段落15-17中任一项所述的工艺的一些实施例中，所述多肽在对应于SEQID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
19.在如段落18所述的工艺的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
20.在如段落15-19中任一项所述的工艺的一些实施例中，其中所述多肽包含SEQID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ IDNO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
21.在另一个方面，一种由淀粉底物生产发酵产物的工艺，所述工艺包括以下步骤：
1)在低于所述淀粉底物的初始糊化温度的温度糖化所述淀粉底物；以及
2)用发酵生物发酵，
其中至少使用选自由以下组成的组的葡糖淀粉酶进行步骤1)：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
22.在如段落21所述的工艺的一些实施例中，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
23.在如段落22所述的工艺的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
24.在如段落21-23中任一项所述的工艺的一些实施例中，所述多肽在对应于SEQID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
25.在如段落24所述的工艺的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
26.在如段落21-25中任一项所述的工艺的一些实施例中，其中所述多肽包含SEQID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ IDNO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
27.在另一个方面，一种用于提高在动物中的淀粉消化率的方法，所述方法包括添加至少一种葡糖淀粉酶，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
28.在如段落27所述的方法的一些实施例中，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
29.在如段落28所述的方法的一些实施例中，其中所述多肽包含取代，该取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
30.在如段落27-29中任一项所述的方法的一些实施例中，所述多肽在对应于SEQID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
31.在如段落30所述的方法的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
32.在如段落27-31中任一项所述的方法的一些实施例中，其中所述多肽包含SEQID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ IDNO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
33.在另一个方面，一种生产发酵饮料的方法，其中所述方法使醪液和/或麦芽汁与葡糖淀粉酶接触的步骤，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
34.在如段落33所述的方法的一些实施例中，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
35.在如段落34所述的方法的一些实施例中，其中所述多肽包含取代，该取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
36.在如段落33-35中任一项所述的方法的一些实施例中，所述多肽在对应于SEQID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
37.在如段落36所述的方法的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
38.在如段落33-37中任一项所述的方法的一些实施例中，其中所述多肽包含SEQID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ IDNO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
39.在另一个方面，一种包含淀粉底物和葡糖淀粉酶的组合物，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性；其中所述组合物的温度是约4℃-40℃并且pH是约3-7。
40.在如段落39所述的组合物的一些实施例中，其中所述多肽在对应于SEQ IDNO:61的多肽的位置102的位置处包含取代、缺失或添加。
41.在如段落40所述的组合物的一些实施例中，其中包含多肽包含取代，包含取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
42.在如段落39-41中任一项所述的组合物的一些实施例中，包含多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
43.在如段落42所述的组合物的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
44.在如段落39-43中任一项所述的组合物的一些实施例中，其中所述多肽包含SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ IDNO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ IDNO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ IDNO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ IDNO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ IDNO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
45.在另一个方面，一种包含葡糖淀粉酶的重组宿主细胞，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
46.在如段落45所述的重组宿主细胞的一些实施例中，其中所述多肽在对应于SEQID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
47.在如段落46所述的重组宿主细胞的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
48.在如段落45-47中任一项所述的重组宿主细胞的一些实施例中，所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
49.在如段落48所述的重组宿主细胞的一些实施例中，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
50.在如段落45-49中任一项所述的重组宿主细胞的一些实施例中，其中所述多肽包含SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ IDNO:66、SEQ ID NO:67、SEQ ID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ IDNO:72、SEQ ID NO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ IDNO:78、SEQ ID NO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ IDNO:84、SEQ ID NO:85、SEQ ID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ IDNO:90、SEQ ID NO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ IDNO:96、SEQ ID NO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
51.在如段落45-50中任一项所述的重组宿主细胞的一些实施例中，所述重组宿主细胞是产乙醇微生物。
52.在如段落51所述的重组宿主细胞的一些实施例中，所述重组宿主细胞是酵母细胞。
53.在如段落45-52中任一项所述的重组宿主细胞的一些实施例中，其中所述宿主细胞不是瓶霉(Saksenaea vasiformis)。
本文所述的方面和实施例中的每个能够一起使用，除非明确地或清楚地从实施例或方面的上下文中排除。
在整个说明书中，引用了各种专利、专利申请和其他类型的出版物(例如，期刊文章、电子数据库条目等)。出于所有目的，本文所引用的所有专利、专利申请和其他出版物的公开内容通过引用以其全文特此并入。
附图说明
图1提供了毛霉菌目(Mucorales)进化枝葡糖淀粉酶和各种参考真菌葡糖淀粉酶的催化结构域区域的多重氨基酸序列比对。
图2提供了毛霉菌目进化枝葡糖淀粉酶和其他真菌葡糖淀粉酶的系统发生树。
图3提供了毛霉菌目进化枝GA氨基酸序列(根据SvaGa1催化结构域区域进行编号，SEQ ID NO:81)在跨残基50至70的区域的比对，该区域显示基序1：57Y-58Xa-59Xb-60T-61X-62X-63Xc-64Xd，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S。
图4提供了毛霉菌目进化枝GA氨基酸序列(根据SvaGa1催化结构域区域进行编号，SEQ ID NO:81)在跨残基240至260的区域的比对，该区域显示基序2：244Xa-245Xb-246Xc-247Xc-248A-249A-250N-251X-252Xd，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G。
图5提供了毛霉菌目进化枝GA氨基酸序列(根据SvaGa1催化结构域区域进行编号，SEQ ID NO:81)在跨残基299至315的区域的比对，该区域显示基序3：304Xa-305G-306X-307G-308N-309Xb-310Xc，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E。
图6提供了另外的毛霉菌目进化枝葡糖淀粉酶和各种参考真菌葡糖淀粉酶的催化结构域区域的多重氨基酸序列比对。
图7提供了另外的毛霉菌目进化枝葡糖淀粉酶和其他真菌葡糖淀粉酶的系统发生树。
具体实施方式
本公开涉及重组宿主细胞、包含葡糖淀粉酶的组合物和使用葡糖淀粉酶糖化淀粉底物的方法。此外，本公开还涉及生产发酵产物的工艺和用于提高在动物中的淀粉消化率的方法以及生产发酵饮料的方法。
I.定义
在详细描述组合物和方法之前，定义了如下的术语和缩写。
除非另有定义，否则所用的所有技术和科学术语具有其在相关科学领域的普通含义。Singleton,等人,Dictionary of Microbiology and Molecular Biology[微生物学和分子生物学词典],第2版,John Wiley and Sons[约翰·威利父子出版公司],纽约(1994)，以及Hale和Markham,Harper Collins Dictionary of Biology[哈伯科林斯生物学词典],Harper Perennial[哈珀永久出版社],纽约(1991)提供了描述本发明的许多术语的普通含义。
术语“葡糖淀粉酶(1,4-α-D-葡聚糖葡糖水解酶，EC 3.2.1.3)活性”在本文被定义为催化从淀粉或相关寡糖和多糖分子的非还原端释放D-葡萄糖的酶活性。
术语“氨基酸序列”与术语“多肽”、“蛋白质”和“肽”同义，并且可互换地使用。当此类氨基酸序列展现出活性时，它们可以被称为“酶”。使用针对氨基酸残基的常规单字母或三字母密码，采用标准氨基端-至-羧基端取向(即N→C)表示氨基酸序列。
术语“成熟多肽”在本文被定义为在翻译和任何翻译后修饰(如N-末端加工、C-末端截短、糖基化、磷酸化等)之后呈其最终形式的多肽。在一个方面，基于SignalP软件版本4.0(Nordahl Petersen等人(2011)Nature Methods[自然方法],8:785-786)的分析，预测的成熟多肽是SEQ ID NO:61并且SEQ ID NO:41是信号肽。在另一个方面，成熟多肽包含SEQID NO:142的氨基酸位置20-468。在另一个方面，成熟多肽包含SEQ ID NO:142的氨基酸位置21-468。在另一个方面，成熟多肽包含SEQ ID NO:142的氨基酸位置22-468。在另一个方面，成熟多肽包含SEQ ID NO:142的氨基酸位置23-468。在另一个方面，成熟多肽包含SEQID NO:142的氨基酸位置24-468。在另一个方面，成熟多肽包含SEQ ID NO:142的氨基酸位置25-468。
“信号序列”或“信号肽”是附接到蛋白质的N-末端部分的氨基酸序列，该氨基酸序列有利于蛋白质在细胞外的分泌。细胞外蛋白质的成熟形式缺乏在分泌过程中被切除的信号序列。在一些实施例中，SEQ ID NO:41是信号肽。在其他实施例中，信号肽包含SEQ IDNO:142的氨基酸位置1-20。在其他实施例中，信号肽包含SEQ ID NO:142的氨基酸位置1-21。在其他实施例中，信号肽包含SEQ ID NO:142的氨基酸位置1-22。在其他实施例中，信号肽包含SEQ ID NO:142的氨基酸位置1-23。在其他实施例中，信号肽包含SEQ ID NO:142的氨基酸位置1-24。
术语“核酸”涵盖能够编码多肽的DNA、RNA、异源双链体、以及合成分子。核酸可以是单链的或双链的，并且可以被化学修饰。术语“核酸”和“多核苷酸”可互换地使用。由于遗传密码是简并的，因此可以使用多于一个密码子来编码特定氨基酸，并且本发明的组合物和方法涵盖编码特定氨基酸序列的核苷酸序列。除非另有说明，否则核酸序列以5′-至-3′取向呈现。
术语“编码序列”意指直接指明蛋白产物的氨基酸序列的核苷酸序列。编码序列的边界通常由可读框决定，该可读框通常以ATG起始密码子或替代起始密码子(如GTG和TTG)开始，并且以终止密码子(如TAA、TAG、和TGA)结束。编码序列可以是DNA、cDNA、合成的或重组的核苷酸序列。
术语“cDNA”在本文被定义为可以通过从真核细胞中获得的成熟的已剪接的mRNA分子逆转录制备的DNA分子。cDNA缺乏可以存在于相应基因组DNA中的内含子序列。早先的初始RNA转录物是mRNA的前体，其在呈现为成熟的剪接的mRNA之前要经一系列的步骤进行加工。这些步骤包括通过称为剪接的过程去除内含子序列。因此，衍生自mRNA的cDNA缺乏任何内含子序列。
“合成”分子通过体外化学或酶促合成而不是通过生物产生。
“宿主菌株”或“宿主细胞”是已经引入了表达载体、噬菌体、病毒或其他DNA构建体，包括编码目的多肽(例如，淀粉酶)的多核苷酸的生物。示例性宿主菌株是能够表达目的多肽和/或发酵糖类的微生物细胞(例如，细菌、丝状真菌和酵母)。术语“宿主细胞”包括由细胞产生的原生质体。
术语“表达”是指基于核酸序列产生多肽的过程。该过程包括转录和翻译两者。
术语“载体”是指设计用于将核酸引入一种或多种细胞类型的多核苷酸序列。载体包括克隆载体、表达载体、穿梭载体、质粒、噬菌体颗粒、盒等。
“表达载体”是指包含编码目的多肽的DNA序列的DNA构建体，该编码序列与能够在合适的宿主中实现DNA表达的合适的控制序列可操作地连接。此类控制序列可包括实现转录的启动子、控制转录的任选操纵子序列、编码mRNA上合适的核糖体结合位点的序列、增强子以及控制转录和翻译终止的序列。
术语“控制序列”在本文被定义为包括编码本发明多肽的多核苷酸表达所必需的所有组分。每个控制序列对于编码该多肽的核苷酸序列可以是天然的或外源的，或每个控制序列对于彼此可以是天然的或外源的。此类控制序列包括但不限于，前导序列、多腺苷酸化序列、前肽序列、启动子、信号肽序列和转录终止子。至少，控制序列包括启动子以及转录和翻译终止信号。出于引入有利于将控制序列与编码多肽的核苷酸序列的编码区连接的特异性限制酶切位点的目的，控制序列可以提供有多个接头。
术语“可操作地连接”意指指定组分处于允许它们以预期方式起作用的关系(包括但不限于并列)。例如，调控序列与编码序列可操作地连接，使得编码序列的表达受调控序列的控制。
术语“序列基序”是广泛的并且已被证明或假定具有生物学意义的核苷酸或氨基酸序列模式。在本发明中，序列基序是在毛霉菌目进化枝葡糖淀粉酶中鉴定的氨基酸序列基序。
“生物学活性的”是指具有指定生物活性，如酶活性的序列。
术语“比活性”是指在特定条件下每单位时间通过酶或酶制剂可转化为产物的底物的摩尔数。比活性通常表示为单位(U)/mg蛋白质。
“序列同一性百分比”是指当使用具有默认参数的CLUSTAL W算法比对时，特定序列具有与指定参考序列中的氨基酸残基同一的至少一定百分比的氨基酸残基。参见Thompson等人(1994)Nucleic Acids Res.[核酸研究]22:4673-4680。CLUSTAL W算法的默认参数是：
Figure BDA0003996176830000211
术语“同源序列”在本文被定义为在用SEQ ID NO:61的葡糖淀粉酶的tfasty检索(Pearson,W.R.,1999,在Bioinformatics Methods and Protocols[生物信息学方法和协议]中，S.Misener和S.A.Krawetz,编辑,第185-219页)中具有小于0.001的E值(或期望分数)的预测蛋白。
如本文所用，关于氨基酸残基位置，“对应于”(corresponding to或correspondsto或correspond to)或“对应”是指在蛋白质或肽中所列举位置处的氨基酸残基，或者与蛋白质或肽中所列举的残基类似、同源或等同的氨基酸残基。如本文所用，“对应区域”通常是指相关蛋白质或参比蛋白质中的类似位置。
关于多肽，术语“野生型”、“亲本”或“参照”是指在一个或多个氨基酸位置处不包含人为取代、插入或缺失的天然存在的多肽。类似地，关于多核苷酸，术语“野生型”、“亲本”或“参照”是指不包括人为核苷变化的天然存在的多核苷酸。然而，注意编码野生型、亲本、或参照多肽的多核苷酸不限于天然存在的多核苷酸，并且涵盖编码野生型、亲本、或参照多肽的任何多核苷酸。
短语“同时糖化和发酵(SSF)”是指生物化学品的生产工艺，其中在同一工艺步骤中存在微生物，例如产乙醇微生物和至少一种酶，例如淀粉酶。SSF包括在相同的反应容器中同时将淀粉底物(颗粒状、液化的或溶解的)水解为糖类(包括葡萄糖)和将糖类发酵为醇类或其他的生物化学品或生物材料。
“浆料”是水中含有不溶性淀粉颗粒的水性混合物。
术语“总糖含量”是指包括单糖、寡糖和多糖的淀粉组合物中存在的总可溶性糖含量。
术语“干固体”(ds)是指溶解于水的干固体、分散于水中的干固体或二者的组合。因此干固体包括颗粒状淀粉及其水解产物，包括葡萄糖。
术语“高DS”是指具有大于38％(wt/wt)的干固体含量的水性淀粉浆料。
“聚合度(DP)”是指给定的糖中脱水吡喃葡萄糖单元的数目(n)。DP1的实例是单糖，如葡萄糖和果糖。DP2的实例是二糖，如麦芽糖和蔗糖。DP4+(>DP3)表示聚合度大于3的聚合物。
术语“接触”是指将参考组分(包括但不限于酶、底物和发酵生物)充分靠近放置以影响预期结果，如该酶作用于底物上或发酵生物发酵底物。
如本文所用，术语“酵母细胞”、“酵母菌株”，或简称“酵母”是指来自子囊菌门(Ascomycota)和担子菌门(Basidiomycota)的生物。示例性酵母是来自酵母目(Saccharomycetales)的芽殖酵母。酵母的特定实例是酵母属物种，包括但不限于酿酒酵母(S.cerevisiae)。酵母包括用于生产燃料醇的生物以及用于生产可饮用醇的生物，包括用于制备独特味道的啤酒、葡萄酒和其他发酵饮料的特种和专有酵母菌株。
“产乙醇微生物”是指具有将糖或其他糖类转化为乙醇的能力的微生物。
术语“生物化学品”是指微生物的代谢物，如柠檬酸、乳酸、琥珀酸、谷氨酸一钠、葡糖酸、葡萄糖酸钠、葡萄糖酸钙、葡萄糖酸钾、葡萄糖酸δ-内酯、异抗坏血酸钠、ω3脂肪酸、丁醇、异丁醇、氨基酸、赖氨酸、衣康酸、其他有机酸、1,3-丙二醇、维生素、或异戊二烯、或其他生物材料。
术语“支链淀粉酶”也称为脱支酶(E.C.3.2.1.41，支链淀粉6-葡聚糖水解酶)，其能够水解支链淀粉分子中的α1-6糖苷键。
某些范围在本文中以数值前加术语“约”呈现。术语“约”在本文用于为其后面的准确数字以及接近或近似于该术语后面的数字的数字提供字面支持。在判定数字是否接近或近似于特定叙述的数字时，接近或近似的未叙述的数字可以是在呈现其的上下文中提供特定叙述的数字的实质性等效物的数字。例如，关于数值，术语“约”是指数值的-15％至+15％的范围，除非术语在上下文中另有特别定义。
除非另外说明，否则以下缩写/首字母缩略词具有以下含义：
EC 酶学委员会
CAZy 碳水化合物活性酶
w/v 重量/体积
w/w 重量/重量
v/v 体积/体积
wt％ 重量百分比
℃ 摄氏度
g或gm 克
μg 微克
mg 毫克
kg 千克
μL和μl 微升
mL和ml 毫升
mm 毫米
μm 微米
mol 摩尔
mmol 毫摩尔
M 摩尔
mM 毫摩尔
μM 微摩尔
nm 纳米
U 单位
ppm 百万分率
hr和h 小时
EtOH 乙醇
除非上下文另有明确指示，否则如本文所用，单数术语“一个/一种(a/an)”和“该/所述(the)”包括复数指示物。
应进一步注意，可以撰写权利要求书以排除任何任选的要素。因此，该陈述旨在作为使用与权利要求要素的叙述有关的排他性术语如“单独”、“仅”等或使用“否定型”限定的前提基础。
术语“包含”及其同源词以其包含的含义使用；即相当于术语“包括”及其相应的同源词。进一步注意到，如本文所用，术语“包含(comprising)”意指包括但不限于在术语“包含”之后的一种或多种组分。在术语“包含”之后的一种或多种组分是必需的或强制性的，但是包含该一种或多种组分的组合物可以进一步包括其他非强制性或任选的一种或多种组分。
仍需注意的是，如本文所用，术语“基本上由……组成(consisting essentiallyof)”是指组合物，其中该术语之后的一种或多种组分在存在其他已知的一种或多种组分的情况下为总组合物的小于30％重量的总量，并且不会影响或干扰一种或多种组分的作用或活性。
还要注意的是，如本文所用，术语“由……组成(consisting of)”意指包括且限于术语“由……组成”之后的一种或多种组分。因此，术语“由……组成”之后的一种或多种组分是必需的或强制性的，且组合物中不存在一种或多种其他组分。
在本说明书通篇中给出的每一最大数值限度旨在包括每一较低数值限度，如同此类较低数值限度在本文中明确写出一样。在本说明书通篇中给出的每一最小数值限度将包括每一较高数值限度，如同此类较高数值限度在本文中明确写出一样。在本说明书通篇中给出的每一数值范围将包括落入此类较宽数值范围内的每一较窄数值范围，如同此类较窄数值范围在本文中全部明确写出一样。
除非本文另外定义，否则本文所用的所有技术与科学术语均具有与本发明所属领域的普通技术人员通常理解的相同含义。
术语的其他定义可在本说明书通篇出现。
II.具有葡糖淀粉酶活性的多肽
在第一方面，本发明涉及包含如下氨基酸序列并且具有葡糖淀粉酶活性的多肽，该氨基酸序列与SEQ ID NO:61或SEQ ID NO:142的多肽具有优选地至少70％、至少75％、至少80％、至少85％、至少90％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、和甚至至少99％的氨基酸序列同一性。在另一个方面，本文提供了包含如下氨基酸序列的多肽，该氨基酸序列与包含SEQ ID NO:142的氨基酸位置20-468、SEQ IDNO:142的氨基酸位置21-468、SEQ ID NO:142的氨基酸位置22-468、SEQ ID NO:142的氨基酸位置23-468、SEQ ID NO:142的氨基酸位置24-468、或SEQ ID NO:142的氨基酸位置25-468的多肽具有优选地至少70％、至少75％、至少80％、至少85％、至少90％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、和甚至至少99％的氨基酸序列同一性。
在一些实施例中，多肽包含与SEQ ID NO:61或SEQ ID NO:142的多肽具有至少70％但小于100％的序列同一性的氨基酸序列。在其他实施例中，多肽包含如下氨基酸序列，该氨基酸序列与包含SEQ ID NO:142的氨基酸位置20-468、SEQ ID NO:142的氨基酸位置21-468、SEQ ID NO:142的氨基酸位置22-468、SEQ ID NO:142的氨基酸位置23-468、SEQID NO:142的氨基酸位置24-468、或SEQ ID NO:142的氨基酸位置25-468的多肽具有至少70％但小于100％的序列同一性。在一些实施例中，多肽是非天然存在的(即在自然中不存在并且是人类创造力的产物)。
在一些实施例中，本发明的多肽是包含如下氨基酸序列的同源多肽，这些氨基酸序列与SEQ ID NO:61的多肽、SEQ ID NO:142的多肽、包含SEQ ID NO:142的氨基酸位置20-468的多肽、包含SEQ ID NO:142的氨基酸位置21-468的多肽、包含SEQ ID NO:142的氨基酸位置22-468的多肽、包含SEQ ID NO:142的氨基酸位置23-468的多肽、包含SEQ ID NO:142的氨基酸位置24-468的多肽、包含SEQ ID NO:142的氨基酸位置25-468的多肽相差不超过十个氨基酸、不超过九个氨基酸、不超过八个氨基酸、不超过七个氨基酸、不超过六个氨基酸、不超过五个氨基酸、不超过四个氨基酸、不超过三个氨基酸、不超过两个氨基酸、并且甚至不超过一个氨基酸。
在一些实施例中，本发明的多肽是包含SEQ ID NO:61的氨基酸18至449的催化区域，通过ClustalX安全超文本传输协议://world wide web.ncbi.nlm.nih.gov/pubmed/17846036预测的。
在一些实施例中，本发明的多肽具有支链淀粉水解活性。
在第二个方面，本文公开的葡糖淀粉酶，相对于SEQ ID NO:61的氨基酸序列、SEQID NO:142的多肽、包含SEQ ID NO:142的氨基酸位置20-468的多肽、包含SEQ ID NO:142的氨基酸位置21-468的多肽、包含SEQ ID NO:142的氨基酸位置22-468的多肽、包含SEQ IDNO:142的氨基酸位置23-468的多肽、包含SEQ ID NO:142的氨基酸位置24-468的多肽、包含SEQ ID NO:142的氨基酸位置25-468的多肽包含一个或几个氨基酸残基的一个或多个保守取代。以下列出了示例性保守氨基酸取代。一些保守取代(即突变)可以通过基因操作产生而其他保守取代通过用其他方式将合成的氨基酸引入多肽中产生。
Figure BDA0003996176830000271
在一些实施例中，本发明的多肽是SEQ ID NO:61的多肽、SEQ ID NO:142的多肽、包含SEQ ID NO:142的氨基酸位置20-468的多肽、包含SEQ ID NO:142的氨基酸位置21-468的多肽、包含SEQ ID NO:142的氨基酸位置22-468的多肽、包含SEQ ID NO:142的氨基酸位置23-468的多肽、包含SEQ ID NO:142的氨基酸位置24-468的多肽、或包含SEQ ID NO:142的氨基酸位置25-468的多肽的变体或其具有葡糖淀粉酶活性的片段。该变体葡糖淀粉酶相对于SEQ ID NO:61或SEQ ID NO:142的氨基酸序列或其同源序列包含一个或几个氨基酸残基的缺失、取代、插入或添加。在所有情况下，表述“一个或几个氨基酸残基”是指10个或更少，即1、2、3、4、5、6、7、8、9或10个氨基酸残基。SEQ ID NO:61的多肽、SEQ ID NO:142的多肽、包含SEQ ID NO:142的氨基酸位置20-468的多肽、包含SEQ ID NO:142的氨基酸位置21-468的多肽、包含SEQ ID NO:142的氨基酸位置22-468的多肽、包含SEQ ID NO:142的氨基酸位置23-468的多肽、包含SEQ ID NO:142的氨基酸位置24-468的多肽、包含SEQ ID NO:142的氨基酸位置25-468的多肽的氨基酸取代、缺失和/或插入可以是最多10个、最多9个、最多8个、最多7个、最多6个、最多5个、最多4个、最多3个、最多2个、并且甚至最多1个。
在一些实施例中，变体改变包含在对应于SEQ ID NO:61的多肽的位置102的位置处的取代或由其组成。在一些实施例中，在对应于SEQ ID NO:61的多肽的位置102的位置处的氨基酸被Ala、Arg、Asn、Asp、Cys、Gln、Glu、Gly、His、Leu、Ile、Lys、Met、Phe、Pro、Thr、Trp、Tyr、或Val取代，优选地被Pro取代。在一些实施例中，变体改变包含SEQ ID NO:61的多肽的取代S102P或由其组成。在另外的实施例中，变体包含SEQ ID NO:104或SEQ ID NO:141的氨基酸序列或由其组成。
在一些实施例中，变体改变包含在对应于SEQ ID NO:61的多肽的位置66的位置处的取代或由其组成。在一些实施例中，在对应于SEQ ID NO:61的多肽的位置85的位置处的氨基酸被Ala、Arg、Asn、Asp、Cys、Gln、Glu、Gly、His、Leu、Ile、Lys、Met、Phe、Pro、Ser、Thr、Trp、或Tyr取代。在一些实施例中，变体改变包含SEQ ID NO:61的多肽的取代V66A或由其组成。
在另一个实施例中，变体改变包含在对应于SEQ ID NO:61的多肽的位置66和位置102的位置处的取代或由其组成。在一些实施例中，变体改变包含SEQ ID NO:61的多肽的取代V66A和S102P或由其组成。在另外的实施例中，变体包含SEQ ID NO:140的氨基酸序列或由其组成。
可替代地，这些氨基酸改变具有这样一种性质：改变多肽的物理化学特性。例如，氨基酸改变可以提高多肽的热稳定性、改变底物特异性、改变最佳pH等。
使用已知的诱变、重组和/或改组方法，随后进行相关的筛选程序可以做出单一或多种氨基酸取代、缺失和/或插入并对其进行测试，该相关的筛选程序是例如由Reidhaar-Olson和Sauer,1988,Science[科学]241:53-57；Bowie和Sauer,1989,Proc.Natl.Acad.Sci.USA[美国科学院院报]86:2152-2156；WO 95/17413；或WO 95/22625公开的那些。可以使用的其他方法包括易错PCR、噬菌体展示(例如，Lowman等人,1991,Biochem.[生物化学]30:10832-10837；美国专利号5,223,409；WO 92/06204)以及区域定向诱变(Derbyshire等人,1986,Gene[基因]46:145；Ner等人,1988,DNA 7:127)。
III.葡糖淀粉酶的生产
本发明的葡糖淀粉酶可以在宿主细胞中产生，例如通过分泌或细胞内表达。在将葡糖淀粉酶分泌到细胞培养基中后，可以获得包含葡糖淀粉酶的培养细胞材料(例如，全细胞培养液)。任选地，葡糖淀粉酶可以从宿主细胞中分离，或甚至从细胞培养液中分离，这取决于最终葡糖淀粉酶所需的纯度。可以根据本领域熟知的方法克隆和表达编码葡糖淀粉酶的基因。合适的宿主细胞包括细菌、真菌(包括酵母和丝状真菌)和植物细胞(包括藻类)。特别有用的宿主细胞包括黑曲霉(Aspergillus niger)、米曲霉(Aspergillus oryzae)、里氏木霉(Trichoderma reesi)、或嗜热毁丝霉(Myceliopthora thermophila)。其他宿主细胞包括细菌细胞，例如枯草芽孢杆菌(Bacillus subtilis)或地衣芽孢杆菌(B.licheniformis)，以及链霉菌属(Streptomyces)。合适的酵母宿主生物可以选自裂殖酵母属(Schizosaccharomyces)物种或酵母属(Saccharomyces)物种，包括酿酒酵母(Saccharomyces cerevisiae)或属于裂殖酵母属的物种，如例如，粟酒裂殖酵母(S.pombe)物种。甲基营养型酵母物种菌种巴斯德毕赤酵母(Pichia pastoris)可以用作宿主生物。
另外，宿主可以表达一种或多种辅酶、蛋白质、肽。这些可以有益于液化、糖化、发酵、SSF和下游处理。此外，除了用于消化各种原料的酶之外，宿主细胞还可以产生乙醇和其他生物化学品或生物材料。此类宿主细胞可用于发酵或同时糖化和发酵工艺，以减少或消除添加酶的需要。
A.载体
可以构建包含编码葡糖淀粉酶多肽的核酸的DNA构建体，使得其适合在宿主细胞中表达。由于遗传密码中已知的简并性，编码相同氨基酸序列的不同多核苷酸可以用常规技术进行设计和制备。也已知的是，取决于期望的宿主细胞，在尝试表达之前可能需要密码子优化。
可以将编码本公开的葡糖淀粉酶多肽的多核苷酸结合到载体中。可以使用已知的转化技术(例如以下公开的那些)将载体转移至宿主细胞中。
合适的载体可以是可被转化为宿主细胞和/或在宿主细胞内复制的载体。例如，包含编码本公开的葡糖淀粉酶多肽的核酸的载体可以在作为繁殖和扩增载体的手段的细菌宿主细胞中转化和/或复制。也可以将载体适当地转化到表达宿主内，使得编码多核苷酸表达为功能性葡糖淀粉酶。
代表性的有用载体是pTrex3gM(参见，公开的美国专利申请20130323798)和pTTT(参见，公开的美国专利申请20110020899)，它们可以被插入宿主的基因组中。可以用常规技术修饰载体pTrex3gM和pTTT两者，使得它们包含并表达编码本发明的葡糖淀粉酶多肽的多核苷酸。
表达载体通常包含控制核苷酸序列，如启动子、操纵子、核糖体结合位点、翻译起始信号以及任选地抑制子基因或一个或多个激活子基因。另外，表达载体可包含编码氨基酸序列的序列，该氨基酸序列能够将葡糖淀粉酶靶向宿主细胞细胞器(如过氧化物酶体)或靶向特定宿主细胞区室。对于在控制序列的指导下进行表达，将葡糖淀粉酶的核酸序列以关于表达的适当方式与控制序列可操作地连接。
可以将编码本发明的葡糖淀粉酶多肽的多核苷酸与允许在宿主细胞中转录的启动子可操作地连接。启动子可以是在选择的宿主细胞中显示转录活性的任何DNA序列，并且可衍生自编码与该宿主细胞同源的或异源的蛋白质的基因。用于定向编码葡糖淀粉酶的DNA序列的转录的启动子的实例，特别是在细菌宿主中，包括大肠杆菌(E.coli)的乳糖操纵子的启动子、天蓝色链霉菌(Streptomyces coelicolor)琼脂酶基因dagA或celA启动子、地衣芽孢杆菌(Bacillus licheniformis)淀粉酶基因(amyL)启动子、嗜热脂肪芽孢杆菌(Bacillus stearothermophilus)生麦芽糖淀粉酶基因(amyM)的启动子、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)淀粉酶(amyQ)的启动子、枯草芽孢杆菌(Bacillussubtilis)xylA和xylB基因的启动子等。
对于在真菌宿主中的转录，有用的启动子的实例包括衍生自编码以下的基因的那些：米曲霉TAKA淀粉酶、米黑根毛霉(Rhizomucor miehei)天冬氨酸蛋白酶、黑曲霉中性α-淀粉酶、黑曲霉酸性稳定α-淀粉酶、黑曲霉葡糖淀粉酶、米黑根毛霉脂肪酶、米曲霉碱性蛋白质、米曲霉磷酸丙糖异构酶、构巢曲霉(Aspergillus nidulans)乙酰胺酶等。当编码葡糖淀粉酶的基因在细菌物种(如大肠杆菌)中表达时，可以从，例如，包括T7启动子和噬菌体λ启动子的噬菌体启动子中选择合适的启动子。沿着这些思路，用于在酵母物种中表达的合适启动子的实例包括但不限于酿酒酵母的Gal 1和Gal 10启动子和巴斯德毕赤酵母AOX1或AOX2启动子。在丝状真菌宿主细胞中的表达通常涉及cbh1，其是来自里氏木霉的内源诱导型启动子。参见Liu等人(2008)Acta Biochim.Biophys.Sin(Shanghai)[生物化学与生物物理学报(上海)]40(2):158-65。
编码序列可以与信号序列可操作地连接。编码信号序列的DNA可以是与待表达的目的葡糖淀粉酶基因天然相关的DNA序列或可以是来自与该葡糖淀粉酶不同的属或物种。可以将包含DNA构建体或载体的信号序列和启动子序列引入真菌宿主细胞，并且可以衍生自相同的来源。例如，信号序列可以是与cbh1启动子可操作地连接的里氏木霉(Trichoderma reesei)cbh1信号序列。
表达载体还可以包含合适的转录终止子，以及在真核生物中，包含与编码葡糖淀粉酶的DNA序列可操作地连接的聚腺苷酸化序列。终止序列和聚腺苷酸化序列可以适当地衍生自与启动子相同的来源。
载体还可以包含可选择标记，例如其产物弥补分离的宿主细胞中的缺陷的基因，如来自枯草芽孢杆菌或地衣芽孢杆菌的dal基因，或者赋予抗生素抗性(如例如氨苄青霉素、卡那霉素、氯霉素或四环素抗性)的基因。此外，载体可以包含曲霉属选择标记，如amdS、argB、niaD和xxsC，引起潮霉素抗性的标记，或者选择可以通过共转化(如本领域已知的)实现。参见，例如，公开的国际PCT申请WO 91/17243。
B.宿主细胞的转化和培养
包含DNA构建体或表达载体的分离的细胞有利地用作重组产生葡糖淀粉酶的宿主细胞。通过将DNA构建体(以一或多个拷贝)整合到宿主染色体中，可以方便地用编码酶的DNA构建体转化细胞。通常认为这种整合是有利的，因为DNA序列更可能在细胞中稳定保持。可以根据常规方法，例如，通过同源或异源重组，将DNA构建体整合到宿主染色体中。可替代地，可以用与不同类型的宿主细胞相关的表达载体转化细胞。
合适的细菌宿主生物的实例是革兰氏阳性细菌物种，例如芽孢杆菌属(Bacillaceae)，包括枯草芽孢杆菌、地衣芽孢杆菌、迟缓芽孢杆菌(Bacillus lentus)、短芽孢杆菌(Bacillus brevis)、嗜热脂肪地芽孢杆菌(Geobacillus stearothermophilus)(原嗜热脂肪芽孢杆菌)、嗜碱芽孢杆菌(Bacillus alkalophilus)、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)、凝结芽孢杆菌(Bacillus coagulans)、灿烂芽孢杆菌(Bacillus lautus)、巨大芽孢杆菌(Bacillus megaterium)和苏云金芽孢杆菌(Bacillusthuringiensis)；链霉菌属(Streptomyces)物种，如鼠灰链霉菌(Streptomyces murinus)；乳酸细菌物种，包括乳球菌属物种(Lactococcus sp.)，如乳酸乳球菌(Lactococcuslactis)；乳杆菌属物种(Lactobacillus sp)，包括罗伊氏乳杆菌(Lactobacillusreuteri)；明串珠菌属物种(Leuconostoc sp.；)；片球菌属物种(Pediococcus sp.)；和链球菌属物种(Streptococcus sp.)。可替代地，可以选择属于肠杆菌科(Enterobacteriaceae)(包括大肠杆菌)或假单胞菌科(Pseudomonadaceae)的革兰氏阴性细菌物种作为宿主生物。
适合的酵母宿主生物可以选自生物技术相关的酵母物种，如但不限于酵母物种，如毕赤酵母属物种(Pichia sp.)，汉逊酵母属物种(Hansenula sp.)或克鲁维酵母属(Kluyveromyces)、耶氏酵母属(Yarrowinia)、裂殖酵母属(Schizosaccharomyces)物种或者酵母属(Saccharomyces)物种，包括酿酒酵母(Saccharomyces cerevisiae)，或者属于裂殖酵母属的物种，如例如，粟酒裂殖酵母(S.pombe)物种。甲基营养型酵母物种菌种巴斯德毕赤酵母可以用作宿主生物。可替代地，宿主生物可以是汉逊酵母属(Hansenula)物种。
丝状真菌中合适的宿主生物包括曲霉属(Aspergillus)的物种，例如，黑曲霉、米曲霉、塔宾曲霉(Aspergillus tubigensis)、泡盛曲霉(Aspergillus awamori)或构巢曲霉。可替代地，镰刀菌属(Fusarium)物种(例如，尖孢镰刀菌(Fusarium oxysporum))或根瘤菌属(Rhizomucor)物种(如米氏根瘤菌(Rhizomucor miehei))的菌种可以用作宿主生物。其他合适的菌株包括嗜热菌属(Thermomyces)和毛霉菌属(Mucor)物种。此外，木霉属物种(Trichoderma sp.)可以用作宿主。真菌宿主细胞表达的葡糖淀粉酶可以被糖基化，即，将包含糖基部分。糖基化模式可以与野生型葡糖淀粉酶中存在的相同或不同。糖基化的类型和/或程度可能改变酶和/或生物化学特性。
从表达宿主缺失基因是有利的，其中可以通过转化的表达载体治愈基因缺陷。已知方法可用于获得具有一种或多种失活基因的真菌宿主细胞。已克隆的、来自木霉属物种或其他丝状真菌宿主的任何基因均可以缺失，例如，cbh1、cbh2、egl1和egl2基因。基因缺失可通过本领域已知方法通过将待失活的所需基因的形式插入质粒中来完成。
通用转化技术是本领域已知的。参见，例如Sambrook等人(2001)，同上。异源蛋白质在木霉属中的表达描述于例如美国专利号6,022,725。对于曲霉属菌种的转化，还参考了Cao等人(2000)Science[科学]9:991-1001。可以用载体系统构建遗传稳定的转化体，由此将编码葡糖淀粉酶的核酸稳定整合到宿主细胞染色体中。然后通过已知技术选择并纯化转化体。
C.表达和发酵
产生葡糖淀粉酶的方法可以包括在有利于产生该酶的条件下培养宿主细胞，并从细胞和/或培养基中回收该酶。
用于培养细胞的培养基可以是适合于宿主细胞生长并获得葡糖淀粉酶多肽表达的任何常规培养基。合适的培养基和培养基组分可从商业供应商获得或可以根据公开的配方(例如，如在美国典型培养物保藏中心(American Type Culture Collection)的目录中所述)来制备。
本领域熟知的任何发酵方法都可以合适用来使如上所述的转化的或衍生的真菌菌株发酵。在一些实施例中，真菌细胞在分批或连续发酵条件下生长。
D.用于富集和纯化的方法
分离和浓缩技术是本领域已知的，并且常规方法可用于制备包含本发明的葡糖淀粉酶多肽的浓缩溶液或肉汤。
发酵后，获得发酵液，通过常规分离技术去除微生物细胞和各种悬浮固体(包括剩余的粗发酵材料)，以便获得葡糖淀粉酶溶液。通常使用过滤、离心、微滤、旋转真空鼓式过滤、超滤、离心后超滤、萃取或色谱法等。
有时可能需要浓缩包含葡糖淀粉酶多肽的溶液或肉汤以优化回收。使用未浓缩的溶液或肉汤典型地会增加孵育时间，以便收集富集或纯化的酶沉淀。
IV.组合物
本发明还涉及包含多肽和/或淀粉底物的组合物。在一些实施例中，也可以将包含与SEQ ID NO:61的氨基酸序列具有至少约80％、至少约85％、至少约90％、至少约95％、至少约96％、至少约97％、至少约98％、至少约99％同一性的氨基酸序列的多肽用于酶组合物中。优选地，配制这些组合物以提供所希望的特性，如浅颜色、低气味、以及可接受的储存稳定性(在约4℃-40℃的温度和约3-7的pH下)。
组合物可以包含本发明的多肽作为主要酶组分。可替代地，该组合物可包含多重酶活性，如氨肽酶、淀粉酶、糖酶、羧肽酶、过氨化氢酶、纤维素酶、壳多糖酶、角质酶、环糊精糖基转移酶、脱氧核糖核酸酶、酯酶、α-半乳糖苷酶、β-半乳糖苷酶、α-葡糖苷酶、β-葡糖苷酶、β-淀粉酶、异淀粉酶、卤代过氧化物酶、转化酶、漆酶、脂肪酶、甘露糖苷酶、氧化酶、果胶分解酶、肽谷氨酰胺酶(peptidoglutaminase)、过氧化物酶、植酸酶、多酚氧化酶、蛋白水解酶、支链淀粉酶、核糖核酸酶、转谷氨酰胺酶、木聚糖酶或其组合，该酶可以按本领域技术人员熟知的有效量添加。
可以根据本领域已知的方法来制备多肽组合物，并且这些多肽组合物可以处于液体或干燥组合物的形式。例如，包含本发明葡糖淀粉酶的组合物可以是水性或非水性配制品、颗粒、粉末、凝胶、浆料、糊剂等，这些组合物可进一步包含本文列出的另外的酶中的任何一种或多种，以及缓冲液、盐、防腐剂、水、共溶剂、表面活性剂等。此类组合物可与内源酶或已存在于浆料、水浴、洗衣机、食品或饮料产品等中的其他成分(例如，内源植物(包括藻类)酶，来自先前加工步骤的残留酶等)组合起作用。包括在组合物中的多肽可以根据本领域已知的方法稳定化。
组合物可以是表达多肽的细胞，包括能够从发酵产生产物的细胞。此类细胞能以液体或干燥形式与合适的稳定剂一起提供。此类细胞可以进一步表达另外的多肽，如上面提到的多肽。
以下给出了本发明的多肽或组合物的优选用途的实例。本发明的多肽组合物的剂量以及使用该组合物的其他条件可以基于本领域中已知的方法进行确定。
以上组合物适合在液化、糖化、和/或发酵工艺中使用，优选地在淀粉转化中使用，尤其是用于产生糖浆和发酵产物(如乙醇)。组合物还适用于在动物营养和发酵饮料中使用。
V.用途
本发明还涉及本发明的多肽或组合物在液化工艺、糖化工艺、和/或发酵工艺中的用途。可以在单个工艺中，例如在液化工艺、糖化工艺、或发酵工艺中使用该多肽或组合物。也可以在工艺的组合中(例如在液化和糖化工艺、在液化和发酵工艺中、或者在糖化和发酵工艺中(优选地与淀粉转化有关))使用该多肽或组合物。
A.糖化
可以使用α-淀粉酶和葡糖淀粉酶(任选地在另外一种或多种酶的存在下)，将液化淀粉糖化成富含低DP(例如DP1+DP2)糖的糖浆。糖化的产物的确切组成取决于所使用的酶的组合以及所加工的淀粉的类型。有利地，使用所提供的葡糖淀粉酶可获得的糖浆中含有的DP1占糖化淀粉中总寡糖的重量百分比可以超过90％，例如90％-98％或95％-97％。糖化淀粉中DP2的重量百分比可以尽可能低，低于约3％，例如，0％-3％或0％-2.8％。
液化通常作为连续工艺进行，而糖化通常作为分批工艺进行。糖化条件取决于液化物的性质和可用的酶类型。在一些情况下，糖化工艺可涉及约60℃-65℃的温度和约4.0-4.5的pH(例如pH 4.3)。糖化可以在例如约40℃、约50℃、或约55℃至约60℃或约65℃之间的温度下进行，有必要冷却液化物。可以根据需要调节pH。糖化通常在搅拌槽中进行，这可能需要几个小时来填充或清空。酶典型地以固定比率添加到干燥固体中(槽被填充时)，或者以单剂量添加(在填充阶段开始时)。制备糖浆的糖化反应典型地进行约24-72小时，例如24-48小时。可以在同时糖化和发酵(SSF)中的糖化之前添加预糖化，该预糖化在30℃-65℃之间的温度(典型地约60℃)典型地持续40-90分钟。
B.粗淀粉水解
本发明提供了本发明的葡糖淀粉酶用于从生淀粉或颗粒状淀粉中产生葡萄糖等的用途。通常，本发明的葡糖淀粉酶单独或在α-淀粉酶存在下可用于生淀粉水解(RSH)或颗粒状淀粉水解(GSH)工艺中以产生所需的糖和发酵产物。颗粒状淀粉通过在糊化温度以下酶法水解来溶解。据报道，此类“低温”系统(也称为“无蒸煮”或“冷蒸煮”)能够处理比常规系统更高浓度的干固体(例如，高至45％)。
“生淀粉水解”工艺(RSH)不同于常规淀粉处理工艺，包括典型地在至少葡糖淀粉酶和/或淀粉酶存在下，在淀粉底物的糊化温度或低于淀粉底物的糊化温度顺序或同时糖化和发酵颗粒状淀粉。
本发明的葡糖淀粉酶还可以结合仅水解在包含至少四个葡糖残基的分子中的α-(1,6)-糖苷键的酶来使用。优选地，本发明的葡糖淀粉酶与支链淀粉酶或异淀粉酶组合使用。在G.M.A.van Beynum等人,Starch Conversion Technology[淀粉转换技术],MarcelDekker[马塞尔·德克尔],纽约,1985,101-142中描述了异淀粉酶和支链淀粉酶用于淀粉脱支的用途、酶的分子性质、以及酶与葡糖淀粉酶一起使用的潜在用途。
C.发酵
典型地在约32℃的温度(如从30℃至35℃)，可以通过使淀粉水解产物与发酵生物接触来发酵可溶性淀粉水解产物(特别是富含葡萄糖的糖浆)。“发酵生物”是指适用于发酵工艺并且能够产生所需发酵产物的任何生物(包括细菌和真菌生物)。尤其适合的发酵生物能够直接地或间接地将糖(如葡萄糖或麦芽糖)发酵(即，转化)成所需的发酵产物。发酵生物的实例包括表达乙醇脱氢酶和丙酮酸脱羧酶的酵母(如酿酒酵母)和细菌(例如运动发酵单胞菌(Zymomonas mobilis))。产乙醇微生物可以表达木糖还原酶和木糖醇脱氢酶，这两种酶将木糖转化为木酮糖。例如，可以承受更高温度的改善的产乙醇微生物菌株是本领域已知的并且可以使用。参见Liu等人(2011)Sheng Wu Gong Cheng Xue Bao[生物工程学报]27:1049-56。可以用于醇生产的酵母包括但不限于酵母属物种，包括酿酒酵母、以及克鲁维酵母属(Kluyveromyces)、拉茜斯酵母属(Lachancea)和裂殖酵母属物种。许多酵母菌株是可商购的，其中许多已被选择或基因工程化以获得所需的特征，如高醇生产、快速生长速率等。发酵的温度和pH将取决于发酵生物。通过发酵产生其他代谢物(如柠檬酸和乳酸)的微生物也是本领域已知的。参见，例如，Papagianni(2007)Biotechnol.Adv.[生物技术进展]25:244-63；John等人(2009)Biotechnol.Adv.[生物技术进展]27:145-52。
糖化和发酵工艺可以作为SSF工艺进行。可以用在整个SSF中连续表达和分泌葡糖淀粉酶的真菌细胞进行SSF工艺。表达葡糖淀粉酶的真菌细胞也可以是发酵微生物，例如产乙醇微生物。因此，可以使用表达足够葡糖淀粉酶的真菌细胞进行乙醇生产，从而更少地需要或不需要外源地添加酶。真菌宿主细胞可以选自适当工程化的真菌菌株。除了葡糖淀粉酶之外，还可以使用表达和分泌其他酶的真菌宿主细胞。此类细胞可以表达淀粉酶和/或支链淀粉酶、植酸酶、α-葡糖苷酶、异淀粉酶、β-淀粉酶纤维素酶、木聚糖酶、其他半纤维素酶、蛋白酶、β-葡糖苷酶、果胶酶、酯酶、氧化还原酶、转移酶、或其他酶。发酵后可以回收乙醇。
D.发酵产物
术语“发酵产物”意指通过包括使用发酵生物的发酵工艺的工艺产生的产物。根据本发明考虑的发酵产物包括醇类(例如，阿拉伯糖醇、丁醇、乙醇、丙三醇(glycerol)、甲醇、乙二醇、丙烯乙二醇、丁二醇、甘油(glycerin)、山梨醇、以及木糖醇)；有机酸类(例如，乙酸、醋酮酸、己二酸、抗坏血酸、柠檬酸、2,5-二酮-D-葡糖酸、甲酸、反丁烯二酸、葡糖二酸、葡糖酸、葡糖醛酸、戊二酸、3-羟基丙酸、衣康酸、乳酸、苹果酸、丙二酸、草酸、草酰乙酸、丙酸、丁二酸、以及木糖酸)；酮类(例如，丙酮)；氨基酸类(例如，天冬氨酸、谷氨酸、甘氨酸、赖氨酸、丝氨酸、以及苏氨酸)；烷烃类(例如，戊烷、己烷、庚烷、辛烷、壬烷、癸烷、十一烷、以及十二烷)；环烷烃类(例如，环戊烷、环己烷、环庚烷、以及环辛烷)；烯烃类(例如，戊烯、己烯、庚烯、以及辛烯)；气体类(例如，甲烷、氢气(H2)、二氧化碳(CO2)、以及一氧化碳(CO))；抗生素类(例如，盘尼西林和四环素)；酶类；维生素类(例如，核黄素、B12、β-胡萝卜素)以及激素类。
在优选的方面，发酵产物是乙醇，例如，燃料乙醇；饮用乙醇，即可饮用的中性酒精；或工业乙醇或消费醇类工业(例如啤酒和白酒)、乳制品工业(例如发酵乳制品)、皮革工业和烟草工业中使用的产品。优选使用的发酵工艺包括本领域熟知的醇发酵工艺。优选的发酵工艺为厌氧发酵方法，该方法是为本领域熟知的。
E.酿造
用于制造啤酒的工艺在本领域中是熟知的。参见例如Wolfgang Kunze(2004)“Technology Brewing and Malting[技术酿造与制麦芽]”Research and TeachingInstitute of Brewing,Berlin[柏林酿酒研究与教学研究所](VLB),第3版。简而言之，该工艺涉及：(a)制备醪液，(b)过滤醪液以制备麦芽汁，和(c)发酵麦芽汁以获得发酵饮料(如啤酒)。
可以将包含与淀粉酶和任选地支链淀粉酶和/或异淀粉酶组合的葡糖淀粉酶的酿造组合物添加到上述步骤(a)的醪液中，即在醪液的制备期间添加。可替代地或除此之外，可以将酿造组合物添加到上述步骤(b)的醪液中，即在醪液的过滤期间添加。可替代地或除此之外，可以将酿造组合物添加到上述步骤(c)的麦芽汁中，即在麦芽汁的发酵期间添加。
F.动物营养
本文所述的葡糖淀粉酶和组合物可以用作动物的饲料添加剂以提高淀粉消化率。本文所述的是一种用于提高在动物中的淀粉消化率的方法。
术语“动物”是指属于动物界的任何生物，且包括但不限于哺乳动物(不包括人)、非人动物、家畜、牲畜、农场动物、动物园动物、种畜等。例如，可以提及所有非反刍动物和反刍动物。在实施例中，动物是非反刍动物，即单胃动物。单胃动物的实例包括但不限于猪(pigs和swine)，如仔猪、生长猪、母猪；家禽，如火鸡、鸭、鸡、肉仔鸡、下蛋鸡；鱼，如鲑鱼、鳟鱼、罗非鱼、鲶鱼和鲤鱼；以及甲壳动物，如虾和对虾。在另外的实施例中，该动物是反刍动物，包括但不限于牛、小牛、山羊、绵羊、长颈鹿、北美野牛、驼鹿、麋鹿、牦牛、水牛、鹿、骆驼、羊驼、美洲驼、羚羊、叉角羚和鹿牛羚。
术语“动物饲料”、“饲料”、“喂养料”和“秣料”可互换使用，并且可包含选自下组的一种或多种饲料材料，该组包含：a)谷类，如小粒谷物(例如小麦、大麦、黑麦、燕麦及其组合)和/或大粒谷物，如玉蜀黍或高粱；b)来自谷类的副产物，如玉米麸质粉、含可溶物的干酒糟(DDGS)(特别是基于玉米的含可溶物的干酒糟(cDDGS))、麦麸、粗小麦粉、小麦次粉、米糠、稻壳、燕麦皮、棕榈仁和柑橘渣；c)获自以下来源的蛋白质：如大豆、向日葵、花生、羽扇豆、豌豆、蚕豆、棉花、低芥酸菜籽、鱼粉、干血浆蛋白质、肉和骨粉、马铃薯蛋白、乳清、干椰肉、芝麻；d)获自植物和动物来源的油和脂肪；和/或e)矿物质和维生素。
淀粉在饲料中的消化率高度可变，并且取决于许多因素，包括淀粉和饲料基质两者的物理结构。已经发现，通过使用至少一种葡糖淀粉酶作为饲料添加剂可以改善动物饮食中的淀粉消化率。
当用作或用于制备饲料(例如功能性饲料)时，本发明的酶或饲料添加剂组合物可以与以下中的一种或多种结合使用：营养上可接受的载剂、营养上可接受的稀释剂、营养上可接受的赋形剂、营养上可接受的辅剂、营养活性成分。例如，可以提及选自由以下组成的组的至少一种组分：蛋白质、肽、蔗糖、乳糖、山梨糖醇、甘油、丙二醇、氯化钠、硫酸钠、乙酸钠、柠檬酸钠、甲酸钠、山梨酸钠、氯化钾、硫酸钾、乙酸钾、柠檬酸钾、甲酸钾、乙酸钾、山梨酸钾、氯化镁、硫酸镁、乙酸镁、柠檬酸镁、甲酸镁、山梨酸镁、偏亚硫酸氢钠、对羟基苯甲酸甲酯和对羟基苯甲酸丙酯。
也可能的是，本文所述的至少一种葡糖淀粉酶(或包含如本文所述的至少一种葡糖淀粉酶的酶组合物)可以均化以产生粉末。粉末可与本领域已知的其他组分混合。任选地，喂养料还可含有另外的矿物质(如，例如钙)和/或另外的维生素。在一些实施例中，喂养料为玉米大豆粉混合物。
在替代性优选的实施例中，可以将包含至少一种葡糖淀粉酶的酶组合物配制成如WO 2007/044968(称作TPT颗粒)或W01997/016076或W01992/012645中所述的颗粒，将这些文献通过引用并入本文。“TPT”意指热保护技术。当将饲料添加剂组合物配制成颗粒时，这些颗粒包含包覆在蛋白质核心上的水合屏障盐。这样的盐涂层的优点是改善的耐热性、改善的保藏稳定性和保护免受其他原本会对该酶有不利影响的饲料添加剂的影响。优选地，用于盐涂层的盐在20℃具有大于0.25的水活度或大于60％的恒定湿度。在一些实施例中，盐涂层包含Na2S04。
替代性地，该组合物处于适合于消费的液体制剂中，优选地，此类液体消费品含有以下中的一种或多种：缓冲液、盐、山梨糖醇和/或甘油。
本文所述的任何用作饲料添加剂的葡糖淀粉酶可单独使用或与至少一种直接饲喂的微生物组合使用。DFM的类别包括芽孢杆菌、乳酸菌和酵母。进一步地，本文所述的任何用作饲料添加剂的葡糖淀粉酶可单独使用或与至少一种精油(例如，肉桂醛和/或麝香草酚)组合使用。还进一步地，本文所述的任何用作饲料添加剂的葡糖淀粉酶可单独使用或与至少一种另外的酶组合使用。此类酶的实例包括，但不限于，植酸酶、木聚糖酶、蛋白酶、淀粉酶、葡聚糖酶、或其他葡糖淀粉酶。
还公开了一种用于提高动物饲料的营养价值的方法，其中可将有效量的本文所述的葡糖淀粉酶中的任一种添加到动物饲料中。
如本文所用，短语“有效量”是指单独地或与一种或多种其他活性剂(如但不限于一种或多种另外的酶、一种或多种DFM、一种或多种精油等)组合赋予动物在一个或多个指标上改善的性能所需的活性剂(如本文公开的葡糖淀粉酶多肽中的任一种)的量。
如本文所用，术语“动物性能”可通过任何指标如但不限于饲料效率和/或动物的增重、和/或通过饲料转化率和/或通过饲料中营养物的消化率和/或饲料中可消化能量或代谢能量、和/或通过动物避免疾病负面作用的能力或通过受试对象的免疫应答来确定。
动物性能特性可包括但不限于：体重；增重；质量；体脂百分比；身高；体脂分布；生长；生长速率；蛋大小；蛋重量；蛋质量；产蛋率；矿物质吸收；矿物质排泄、矿物质保留；骨密度；骨强度；饲料转化率(FCR)；平均每日饲料摄入量(ADFI)；平均日增重(ADG)；铜、钠、磷、氮和钙中的任何一种或多种的保留和/或分泌；氨基酸保留或吸收；矿化、骨矿化屠体产量和屠体品质。
“在一个或多个指标上改善的动物性能”意指与不包含饲料添加剂组合物的饲料相比，由于使用本文所述的包含所述饲料添加剂组合物的饲料，受试者中的饲料效率增加和/或增重增加和/或饲料转化率降低和/或饲料中营养物或能量的消化率改善和/或氮保留量改善和/或避免坏死性肠炎负面作用的能力改善和/或免疫应答改善。
出于所有目的，本文引用的所有参考文献均通过引用以其全文并入本文。为了进一步说明组合物和方法及其优点，给出以下特定实例，应理解它们是说明性的而不是限制性的。
实例
实例1
毛霉菌目进化枝葡糖淀粉酶的鉴定
通过扫描标注的接合菌(Zygomycetes)门的蛋白质序列使用dbCAN(Yin等人(2012)“dbCAN:a web resource for automated carbohydrate-active enzymeannoatation:[dbCAN：用于自动化糖类活性酶标注的网络资源].Nucleic Acids Research[核酸研究]40:W4450-451)寻找接合菌门的葡糖淀粉酶，以基于CAZY家族分析鉴定所有GH15蛋白质。在毛霉菌目生物的基因组中鉴定了基因数并进一步分析了序列。从表1中列出的来源中鉴定了编码毛霉菌目进化枝葡糖淀粉酶的基因，并向这些基因分配表1中所示的SEQ ID NO。
Figure BDA0003996176830000431
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Figure BDA0003996176830000441
N-末端信号肽通过SignalP软件版本4.0(Nordahl Petersen等人(2011)NatureMethods[自然方法],8:785-786)预测。编码各种毛霉菌目进化枝葡糖淀粉酶的基因经密码子修饰以用于在里氏木霉中表达。
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Figure BDA0003996176830000451
实例2
毛霉菌目进化枝葡糖淀粉酶在里氏木霉中的表达
编码毛霉菌目进化枝葡糖淀粉酶的基因的多核苷酸(用作表达盒的密码子修饰的序列)(SEQ ID NO:21、SEQ ID NO:22、SEQ ID NO:23、SEQ ID NO:24、SEQ ID NO:25、SEQ IDNO:26、SEQ ID NO:27、SEQ ID NO:28、SEQ ID NO:29、SEQ ID NO:30、SEQ ID NO:31、SEQ IDNO:32、SEQ ID NO:33、SEQ ID NO:34、SEQ ID NO:35、SEQ ID NO:36、SEQ ID NO:37、SEQ IDNO:38、SEQ ID NO:39、和SEQ ID NO:40)由捷瑞(捷瑞生物工程有限公司(Generay BiotechCo.,Ltd)，上海，中国)合成并插入pGX256表达载体(来自pTTT(参见公开的美国专利申请20110020899)的衍生载体)。
构建了编码SvaGA1葡糖淀粉酶(SEQ ID NO:61)的变体的多核苷酸，其中密码子改变引入了一个突变，氨基酸位置102的Ser被Pro代替(SvaGA1v2，S102P)。将编码SvaGA1v2的表达盒插入pGX256(如上所述)。
使用原生质体转化将所有质粒转化到合适的里氏木霉菌株中(Te’o等人,J.Microbiol.Methods[微生物方法杂志]51:393-99,2002)。通过WO 2016/138315中描述的方法选择并发酵这些转化体。通过SDS-PAGE分析，将来自这些培养物的上清液用于确认蛋白质表达。
如Lv等人(2012)在“Construction of teo vectors for gene expression inTrichoderma reesei[用于在里氏木霉中基因表达的teo载体的构建]”Plasmids[质粒]67:67-71中所述，将真菌细胞培养物在确定成分培养基中生长。96小时后，通过离心收集澄清的培养液。通过本领域已知的方法纯化毛霉菌目进化枝葡糖淀粉酶。使用配备有10K MWCO的Amicon Ultra-15设备将含有靶蛋白的柱层析级分合并、浓缩并平衡至20mM乙酸钠(pH5.0)、150mM氯化钠。经纯化的样品纯度大约为99％(通过SDS-PAGE分析)，并在-80℃下储存在40％的甘油中直至使用。
实例3
SvaGA1和SvaGA1v2葡糖淀粉酶在pH 4.5、60℃下糖化的评估
在pH 4.5和60℃评估SvaGA1和SvaGA1v2变体的糖化性能。将GC126(杜邦公司(DuPont)/IFF(国际香精香料公司)产品)预处理的玉米淀粉液化物(在38％ds、pH 3.3制备)的样品用作起始底物。在30μg/gds的剂量下测试葡糖淀粉酶的性能。包括来自
Figure BDA0003996176830000461
XTRA(诺维信公司(Novozymes)产品)的葡糖淀粉酶密褐褶菌(Gloeophyllumtrabeum)葡糖淀粉酶(GtGA)以进行比较。对于此评估，用于每次孵育的支链淀粉酶OPTIMAXTM L 1000(杜邦公司产品)以10μg/gds给药，并且α-淀粉酶白曲霉(Aspergilluskawachii)淀粉酶(AkAA，描述于WO 2013169645中，将其通过引用并入本文)以5μg/gds给药。将玉米淀粉液化物底物和酶(葡糖淀粉酶、α-淀粉酶和支链淀粉酶)在pH 4.5、60℃分别孵育48小时和65小时。通过在100℃加热15min来淬灭所有孵育。取出等分试样并在5mMH2SO4中稀释40倍，以通过使用配备有Phenomenex Rezex-RFQ Fast Fruit柱(cat#00D-0223-K0)的安捷伦(Agilent)1200系列系统的HPLC，在80℃运行进行产物分析。将10μL样品加载到柱上，并用等梯度的5mM H2SO4作为流动相以1.0mL/min的流速分离。使用折光率检测器检测寡糖产物，并且运行标准溶液以确定每种目的DP(n)糖(DP3+、DP3、DP2和DP1)的洗脱时间。表3中显示的值反映了作为总DP1至DP3+的分数的每个DP(n)的峰面积百分比。在pH4.5、60℃，通过SvaGA1和SvaGA1v2葡糖淀粉酶的DP1产生和DP3+水解的结果优于参考GtGA酶的那些。
Figure BDA0003996176830000471
实例4
毛霉菌目进化枝葡糖淀粉酶在可溶性淀粉上的比活性
使用偶联的葡萄糖氧化酶/过氧化物酶(GOX/HRP)和2,2'-联氮基-双3-乙基苯噻唑啉-6-磺酸(ABTS)法(Anal.Biochem.[分析生物化学]105(1980),389-397)，基于从可溶性淀粉释放的葡萄糖来测定葡糖淀粉酶比活性。通过将9mL可溶性淀粉(1％水溶液，w/w)和1mL 0.5M pH 5.0的乙酸钠缓冲液在15mL锥形管中混合来制备底物溶液。在50mM乙酸钠缓冲液(pH 5.0)中制备具有ABTS的偶联酶(GOX/HRP)溶液，其中终浓度为2.74mg/mL ABTS、0.1U/mL HRP、和1U/mL GOX。在纯化水中制备待评估的每种葡糖淀粉酶样品和葡萄糖标准品的连续稀释液。将每种葡糖淀粉酶样品(10μL)转移到新的含有90μL在50℃下以600rpm预孵育5min的底物溶液的微量滴定板(康宁公司(Corning)3641)中。反应在50℃下进行10min(在热混合器(Eppendorf)中振荡(600rpm))，将10μL反应混合物以及10μL葡萄糖标准品的连续稀释液分别快速转移至新的微量滴定板(康宁公司3641)，然后，添加100μL ABTS/GOX/HRP溶液。立即使用SoftMax Pro酶标仪(分子装置公司(Molecular Device))以11秒间隔持续5min测量405nm处的吸光度。输出是每个酶浓度的反应速率(Vo)。线性回归用于确定Vo相比于酶剂量的曲线的斜率。使用以下等式基于葡萄糖标准曲线计算每种葡糖淀粉酶的比活性：
比活性(单位/mg)＝斜率(酶)/斜率(标准)×1000(1)，
其中1单位＝1μmol葡萄糖/分钟。
使用上述方法，确定毛霉菌目进化枝葡糖淀粉酶和基准的比活性。
结果示于表4中。
Figure BDA0003996176830000481
实例5
同源毛霉菌目进化枝葡糖淀粉酶序列的分析
针对涵盖毛霉菌目进化枝葡糖淀粉酶的催化结构域的区域，构建多重氨基酸序列比对：SvaGA1 SEQ ID NO:81、BciGA1 SEQ ID NO:82、BciGA2 SEQ ID NO:83、BpoGA1 SEQID NO:84、CcuGA1 SEQ ID NO:85、RstGA1 SEQ ID NO:86、MciGA5 SEQ ID NO:87、DelGA1SEQ ID NO:88、FspGA3 SEQ ID NO:89、GpeGA1 SEQ ID NO:90、MciGA3 SEQ ID NO:91、CumGA1 SEQ ID NO:92、McoGA1 SEQ ID NO:93、ParGA1 SEQ ID NO:94、RmiGA1 SEQ ID NO:95、SfuGA2 SEQ ID NO:96、SraGA1 SEQ ID NO:97、SraGA3 SEQ ID NO:98、TinGA1 SEQ IDNO:99、和ZmeGA1 SEQ ID NO:100。在SvaGa1情况下，催化结构域的长度为432个残基并且跨越预测的成熟蛋白质序列的氨基酸18至449。基于泡盛曲霉(A.awamori)(Aleshin等人,1994,J.Mol.Bio[分子生物学杂志].238:575-591)和黑曲霉(Lee和Paetzel,2011,ActaCryst[晶体学报]F67:188-192)葡糖淀粉酶序列的研究，上述区域与先前定义的真菌葡糖淀粉酶的催化结构域重叠。使用Geneious 10.2软件内的MUSCLE比对工具(默认参数)来比对这些序列。在公共结构域中鉴定了另外的同源序列：GAN00808.1 SEQ ID NO:101、ORE14155.1 SEQ ID NO:102、和RCH88939.1 SEQ ID NO:103，并且它们的重叠序列也包括在此分析中。此外，以下比对中包括其他葡糖淀粉酶(不是真菌的毛霉菌目成员)的催化结构域：黑曲霉葡糖淀粉酶(AnGA)SEQ ID NO:105、烟曲霉(Aspergillus fumigatus)葡糖淀粉酶(AfuGA)SEQ ID NO:106、根状索孔菌(Fibroporia radiculosa)TFFH 294葡糖淀粉酶(FraGA1)SEQ ID NO:107、轮枝样镰刀菌(Fusarium verticillioides)葡糖淀粉酶(FveABC11)SEQ ID NO:108、密褐褶菌葡糖淀粉酶(GtGA)SEQ ID NO:109、草酸青霉菌(Penicillium oxalicum)葡糖淀粉酶(PoxGA)SEQ ID NO:110、里氏木霉葡糖淀粉酶(TrGA)SEQ ID NO:111、和茯苓(Wolfiporia cocos)MD-104SS10葡糖淀粉酶(WcoGA1)SEQ ID NO:112，以确定具有序列相似性和差异的区域。多重序列比对示于图1的分图A-K。使用Geneious 10.2软件由图1的比对生成系统发生树并示于图2。
在图1A-图1K显示的多重序列比对中观察到一系列插入和缺失以及高序列变异性区域。毛霉菌目进化枝葡糖淀粉酶中的几个高相似性区域变得明显，并且已鉴定了序列基序。图3、图4和图5显示3种不同的毛霉菌目进化枝葡糖淀粉酶催化结构域的区域的比对，并突出显示共同的序列基序。图3显示毛霉菌目进化枝GA氨基酸序列(根据SEQ ID NO:81进行编号)在跨残基50至70的区域的比对，突出显示毛霉菌目进化枝GA序列基序1(SEQ ID NO:113)：57Y-58Xa-59Xb-60T-61X-62X-63Xc-64Xd，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S。此区域进一步细化的基序是毛霉菌目进化枝GA基序1A(SEQ ID NO:114)：57Y-58N-59T-60T-61X-62A-63G-64D，其中X是任何氨基酸。图4显示毛霉菌目进化枝GA氨基酸序列(根据SEQ ID NO:81进行编号)在跨残基240至260的区域的比对，描述了毛霉菌目进化枝GA序列基序2(SEQ ID NO:115)：244Xa-245Xb-246Xc-247Xc-248A-249A-250N-251X-252Xd，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G。此区域进一步细化的基序是毛霉菌目进化枝GA序列基序2A(SEQ ID NO:116)：244S-245T-246L-247I-248A-249A-250N-251X-252A，其中X是任何氨基酸。图5显示毛霉菌目进化枝GA氨基酸序列(根据SEQ ID NO:81进行编号)在跨残基299至315的区域的比对，描述了毛霉菌目进化枝GA序列基序3(SEQ ID NO:117)：304Xa-305G-306X-307G-308N-309Xb-310Xc，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E。此区域进一步细化的基序是毛霉菌目进化枝GA基序3A(SEQ ID NO:118)：304N-305G-306N-307G-308N-309S-310Q。
实例6
葡糖淀粉酶的热稳定性评估
在60℃的稳定性比较
比较SvaGA1v2和SvaGA1v3的热稳定性，其中将酶样品(20ppm)在60℃预孵育10min。将在4℃预孵育10min包括在内，并且设置为每个葡糖淀粉酶样品的100％活性。然后使用如实例4中所述的相同的方法(除了pH是4.5并且孵育温度是60℃)测量预孵育后葡糖淀粉酶的残余活性。如表5所示，在这些条件下，SvaGA1v3保留了55％的活性。
Figure BDA0003996176830000511
使用DSC的Tm测量
使用超灵敏MicroCalTM VP-毛细管DSC系统(通用电气医疗公司(GE Healthcare))进行差示扫描量热法(DSC)测量。将纯化的SvaGA1、SvaGA1v2和SvaGA1v3在100mM、pH 4.5的乙酸钠缓冲液中稀释至0.4mg/mL的终浓度。将400μL酶溶液以及含有相同量的无酶缓冲液的参考样品添加到96孔板中。然后，将该板放置在保持在10℃的热控自动进样器室中。分别将酶样品和缓冲液参考样品以2℃/分钟的扫描速率从20℃至100℃扫描。将Tm确定为从折叠状态到未折叠状态的转变的最大峰处的温度。Tm的最大变化为±0.2℃。将ORIGIN软件包(微量热公司(MicroCal)，通用电气医疗公司)用于基线减法和数据的图示。
表6中的DSC结果显示SvaGA1v3的Tm比SvaGA1v2的高3度。
Figure BDA0003996176830000512
实例7
糖化活性确定
在pH 4.5，分别在60℃、62℃、65℃评估SvaGA1v2和SvaGA1v3的糖化性能。所有的孵育条件都与实例3中所述的相同，除了玉米淀粉液化物购买自嘉吉公司(Cargill)。支链淀粉酶OPTIMAXTM L 1000(杜邦公司产品)以4μg/gds给药，并且α-淀粉酶土曲霉(Aspergillus terreus)淀粉酶(AtAA，描述于，例如，国际专利申请公开号WO 2017112635A1和WO 2014099415，通过引用并入本文)以1μg/gds给药。表7中显示的值反映了作为总DP1至DP3+的分数的每个DP(n)的峰面积百分比。在所有选择的条件下，当与参考GtGA酶比较时，通过SvaGA1v3进行的DP1产生和DP3+水解表明优越的性能。
Figure BDA0003996176830000521
实例8
另外的同源毛霉菌目进化枝葡糖淀粉酶的鉴定
在毛霉菌目生物的基因组中鉴定了基因数并进一步分析了序列。从表8中列出的来源鉴定了编码毛霉菌目进化枝葡糖淀粉酶的基因，并且向这些基因分配表9中显示的SEQID NO。
Figure BDA0003996176830000531
N-末端信号肽通过SignalP软件版本4.0(Nordahl Petersen等人(2011)NatureMethods[自然方法],8:785-786)预测。编码各种毛霉菌目进化枝葡糖淀粉酶的基因经密码子修饰以用于在里氏木霉中表达。至少部分基于此分析，制备了名为SvaGA1v3的SvaGA1的新变体，并且向该变体分配表9中显示的SEQ ID NO:140。
Figure BDA0003996176830000532
Figure BDA0003996176830000541
实例9
毛霉菌目进化枝葡糖淀粉酶在里氏木霉中的表达
编码毛霉菌目进化枝葡糖淀粉酶基因(SEQ ID NO:124、SEQ ID NO:127、SEQ IDNO:130、SEQ ID NO:133、SEQ ID NO:136)的多核苷酸(用作表达盒的密码子修饰的序列)由捷瑞(捷瑞生物工程有限公司，上海，中国)合成并插入pGX256表达载体(来自pTTT(参见公开的美国专利申请20110020899)的衍生载体)。
使用原生质体转化将所有质粒转化到合适的里氏木霉菌株中(Te’o等人,J.Microbiol.Methods[微生物方法杂志]51:393-99,2002)。通过WO 2016/138315中描述的方法选择并发酵这些转化体。通过SDS-PAGE分析，将来自这些培养物的上清液用于确认蛋白质表达。
如Lv等人(2012)在“Construction of teo vectors for gene expression inTrichoderma reesei[用于在里氏木霉中基因表达的teo载体的构建]”Plasmids[质粒]67:67-71中所述，将真菌细胞培养物在确定成分培养基中生长。96小时后，通过离心收集澄清的培养液。通过本领域已知的方法纯化毛霉菌目进化枝葡糖淀粉酶。使用配备有10K MWCO的Amicon Ultra-15设备将含有靶蛋白的柱层析级分合并、浓缩并平衡至20mM乙酸钠(pH5.0)、150mM氯化钠。经纯化的样品纯度大约为99％(通过SDS-PAGE分析)，并在-80℃下储存在40％的甘油中直至使用。
实例10
糖化中另外的同源毛霉菌目进化枝葡糖淀粉酶的评估
在pH 4.5，分别在60℃、62℃和65℃评估另外的同源毛霉菌目进化枝葡糖淀粉酶的糖化性能，持续48h。所有孵育条件都和实例7中所述的相同，除了葡糖淀粉酶样品以25μg/gds给药。表10中显示的值反映了作为总DP1至DP3+的分数的每个DP(n)的峰面积百分比。当在pH 4.5，60℃持续48h评估时，当与参考GtGA酶相比时，通过毛霉菌目进化枝葡糖淀粉酶的DP1产生和DP3+水解的结果表明优越的性能。当孵育温度升至62℃时，所有毛霉菌目进化枝GA也显示出比GtGA酶更好的糖化性能。当孵育温度升至65℃时，AtrGA1可维持其优越性能，而GtGA不能。
Figure BDA0003996176830000551
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实例11
另外的同源毛霉菌目进化枝葡糖淀粉酶序列的序列分析
构建涵盖毛霉菌目进化枝葡糖淀粉酶的催化结构域的区域的多重氨基酸序列比对：如实例5中所述的SvaGA1 SEQ ID NO:81、SobGA1 SEQ ID NO:119、AosGA3 SEQ ID NO:120、AelGA1 SEQ ID NO:121、AvaGA1 SEQ ID NO:122、和AtrGA1 SEQ ID NO:123。使用Geneious 10.2软件内的MUSCLE比对工具(默认参数)来比对这些序列。此外，以下比对中包括其他葡糖淀粉酶(不是真菌的毛霉菌目成员)的催化结构域：黑曲霉葡糖淀粉酶(AnGA)SEQ ID NO:105、烟曲霉葡糖淀粉酶(AfuGA)SEQ ID NO:106、根状索孔菌TFFH 294葡糖淀粉酶(FraGA1)SEQ ID NO:107、轮枝样镰刀菌葡糖淀粉酶(FveABC11)SEQ ID NO:108、密褐褶菌葡糖淀粉酶(GtGA)SEQ ID NO:109、草酸青霉菌葡糖淀粉酶(PoxGA)SEQ ID NO:110、里氏木霉葡糖淀粉酶(TrGA)SEQ ID NO:111、和茯苓MD-104SS10葡糖淀粉酶(WcoGA1)SEQ IDNO:112，以确定具有序列相似性和差异的区域。多重序列比对如图6的分图A-D所示。另外的新同源物(SobGA1、AosGA3、AelGA1、AvaGA1、AtrGA1)落在序列SEQ ID NO:113、SEQ ID NO:115、和SEQ ID NO:117中概述的基序内。使用Geneious 10.2软件从以下序列的比对生成系统发生树：SvaGA1 SEQ ID NO:81、BciGA1 SEQ ID NO:82、BciGA2 SEQ ID NO:83、BpoGA1SEQ ID NO:84、CcuGA1 SEQ ID NO:85、RstGA1 SEQ ID NO:86、MciGA5 SEQ ID NO:87、DelGA1 SEQ ID NO:88、FspGA3 SEQ ID NO:89、GpeGA1 SEQ ID NO:90、MciGA3 SEQ ID NO:91、CumGA1 SEQ ID NO:92、McoGA1 SEQ ID NO:93、ParGA1 SEQ ID NO:94、RmiGA1 SEQ IDNO:95、SfuGA2 SEQ ID NO:96、SraGA1 SEQ ID NO:97、SraGA3 SEQ ID NO:98、TinGA1 SEQID NO:99、ZmeGA1 SEQ ID NO:100、GAN00808.1 SEQ ID NO:101、ORE14155.1 SEQ ID NO:102、和RCH88939.1 SEQ ID NO:103、黑曲霉葡糖淀粉酶(AnGA)SEQ ID NO:105、烟曲霉葡糖淀粉酶(AfuGA)SEQ ID NO:106、根状索孔菌TFFH 294葡糖淀粉酶(FraGA1)SEQ ID NO:107、轮枝样镰刀菌葡糖淀粉酶(FveABC11)SEQ ID NO:108、密褐褶菌葡糖淀粉酶(GtGA)SEQ IDNO:109、草酸青霉菌葡糖淀粉酶(PoxGA)SEQ ID NO:110、里氏木霉葡糖淀粉酶(TrGA)SEQID NO:111、和茯苓MD-104SS10葡糖淀粉酶(WcoGA1)SEQ ID NO:112、SobGA1 SEQ ID NO:119、AosGA3 SEQ ID NO:120、AelGA1 SEQ ID NO:121、AvaGA1 SEQ ID NO:122、和AtrGA1SEQ ID NO:123，并示于图7中。
序列表
<110> 美国丹尼斯科公司（Danisco Us Inc.）
<120> 葡糖淀粉酶及其使用方法
<130> NB41777-WO-PCT
<160> 118
<170> PatentIn 3.5版
<210> 1
<211> 1633
<212> DNA
<213> 瓶霉（Saksenaea vasiformis）
<400> 1
atgccatcct ggaaaaccct tttcttgctc cttgggccga ttgcgactgc agcagctgct 60
ccagtggaca agcagtctct tccaaccgga aattctacca ttagctcctg ggtatccaaa 120
caagaagata ttagcttttc ggaaatgctt cgcaatgtaa accctgaggg aactgcaaaa 180
ggatttgtag ctgcatcttt atccaccgcc ggcccagatt acttctacac atggacacgc 240
gacgctgctt tggtttctcg ggtgattgct tacaagtaca acaccaccaa cgctggcgac 300
agtaagatcc acggggtcct tgatgattat gtcaacttcc aaatcaacac gcaatcggag 360
agtacccctt gcaattgcct tggcgaacca aaatttaatc ccgatggatc aagctttacg 420
ggcccctggg gaaggtaata ctgacctcat tgtgcatggg aaagatacta aataattatt 480
tgttgaaact catcagacca caaaacgatg gtccagctga gcgagcttca agcttcatgt 540
tgattgccga tagcttcctg agccaaacaa aaaatgcgtc gtacttcacg aatacattaa 600
agccaggtac gtctaacctc tccggaaata cgttttcccc tttaatcacc gtctttatta 660
gctatctata aggatctcga ttacgtcgtc gatacctgga gcaatccatg cttcgatctg 720
tggtaagcac acacacttta aaaagttggc gaataaggcc tctaattata ttatagggag 780
gaggtcaatg gtatccattt ctataccttg atggtgatgc gacgtagcct cttggatggt 840
gcaaactttg ccacgcgtaa tggtgacaac tccaaggcca gtacctatag tggcgtcgct 900
gccaaaatcc aagcaagact taattccttc tgggacgctg gcaagaatta tattactgtc 960
acacaagact ataagaacgg tgtcgaaaag ccatctggat tggatgtctc tacattgatt 1020
gctgctaatg tagcaggcat gggcgatggt aacaataacg tagtaaagtc tccaacctta 1080
tactgacata ctattttgct taggcttcta cactcccgga tctgaaagaa ttctcgcaac 1140
tgctctagcc ttcgagaagt ccatggcaag tctataccct cttaacaaca atttgccttc 1200
tcaccttggt aatgccatcg gtcgctaccc tgaagacact tacaatggca acggaaattc 1260
ccagggcaac ccgtggttcc tggctactac tgcctttaca gaactttact atcgtgccat 1320
tctcgaatgg aagaacacag gtgtgacagt gacgccaatc agcaaagact tctttgtcag 1380
atttgattcc agtgctgcac ccggcaagaa atacaacccc ggctctcaag aatttgccac 1440
gttgacccag agcatcgccg cagctgccga ccgttttatg tcgacagtac aatatcacca 1500
gaatccaaac ggatctcttt ctgaggagtt tgaccgctcc tcaggataca tgaccggtgc 1560
tcgcgatctc acctggtccc acgctgcttt tatcacagct gctcaagcaa gagctggctc 1620
accatccttc taa 1633
<210> 2
<211> 2498
<212> DNA
<213> 螺旋巴克斯霉（Backusella circina）
<400> 2
atgaaacttt ttggtactct aaaggcatct tttgtcttcc ttattggatg catagtgttt 60
gctatggcag acactgtccc tactacccag gttaaactcc agtcctacac ttattctgga 120
ggtgtattgt ctggtacaat ctatgtaaga gaatgattgc ccaagtctat ttcgtttttt 180
tctcttccaa actcatatat tattttgata ggttcaaaat atagactaca ctaaggttgt 240
cactgttatc tattctgatg gttctggtaa ctggaataac aacggcaata ctatctccgc 300
ttcctactct tcctccattt ctggaacaaa ctatgaatac tggacattct cagcatcagt 360
tagtagcatc cagcaatttt acatctcagt aagtgtaaaa cacaaaaaaa gtaggaaatt 420
tcagttattt atgcattaat cgattctttc tttttttttt agtatcttgt agatggtact 480
acctattatg acaacaatgg tggctatggc gccaactatg ctggtaagct caaattcaaa 540
ttttattttc atttacttaa agatgaataa atagtcactg caaccaccac aactgcttca 600
tctactacta aaaccacttc tggtacaaca aagacatcta ctacgtcaac agccactgct 660
acttcaacag gcacatcctc ttttccttct ggtaattcta ccatcagtac ttggagcccc 720
agtcaacaca gtatcagtct ttacgctatg cttcgtaata tcaatcctcc aggaagtgct 780
gctggattca tttctgcctc cttgtctacc tctggacctg attattatta ttcctggaca 840
agagtaaata gttaaaattt aaaatacatc atgctgtttg agtgctcatt ttttgatatt 900
taggattctg cacttgttgc tcatgtcatt gtcaacgaat ataatactac atatcaagga 960
aacagcaccc ttctcggtat tttgaaggat tatgttacct attctcttaa cgctcaaact 1020
acctctactg tttgtaactg tcttggtgag ccaaaattca accctgatgg ttcgagcttc 1080
actggtgcct ggggaaggta aacaagattc atgaagagag acgaaaagat attaaccgtt 1140
tatttagacc tcaaaatgat ggtccagctg agcgtgctgt cagttttatt tattttgcag 1200
atagttatct cactcaaact agcgactcat cttatgttac tggcacactt gcacctggta 1260
agagtcaatt ttttttatga tttttgcaaa tttcaggaat cttattttgt ttcataagct 1320
atttataagg atttggatta tgttgttagc gtatggagta acggttgttt cgatctttgg 1380
taaattgtat ttactttttt ccttaattgt aatactaata tatgtttgaa tttttttttt 1440
agggaggaag taaatggtat ccatttttac acactgatgt ttatgcgcag aggtctcctt 1500
gatggagcca actttgcctc acgaaacggt gactctactc gtgcttccac ttatacctct 1560
accgctgctt ctattaagac caagattgat ggcttctggg tatcgagtgg aaactatgtt 1620
caggtcagtc aaagtgttac aagcggtgtc agcaaagctg gatatgacgc ttctacctta 1680
attgctgcaa atcaagccag tcggggtgat ggtaagttgt gtcttttttt ttttaccaaa 1740
aaataaaaaa aagaatctca attaaacaaa attgatgttt aggtttctat acacctggat 1800
ccgataaggt aacgcaatag ataaagttaa aacctctata tgactaacat aaagattaca 1860
gatgctggct acagctgttg ctattgaatc ccaattctca agcttgtact caatcaacac 1920
caacaaggct tcttatctag gaaatgctat tggcaggtga gttcctttaa cactacattt 1980
tcttctaaaa ttctgataat atcatattaa taccttatta aaagatatcc cgaagatacc 2040
tataacggca atggtaactc tcaaggcaac ccttggttct tgtaagtaaa tcattctcat 2100
aaattatata aattgcatat ctttaatttt atttttataa aaggtgcact aatgcatttg 2160
gagagctttt ctatcgcgcc atcagtgaat ggaacgctgc tggtagcgtc accgtaaaca 2220
gcgtcaatct tgccttcttc aagaaatacg attcttctac ctcctctggt acaacgtata 2280
cagttggaac aagtgcttac aataatctcg ttcagaatgt tgctcttgct gctgatgcct 2340
acttctccac cgtcaaatat catgctctta ctaatggttc catgtctgaa caatacgatc 2400
gtagctctgg tatggctaca ggtgcaagag atcttacatg gtcacacgcc gctatgatta 2460
ctgccttgaa ggcaaaatct ggaacaccag tttactaa 2498
<210> 3
<211> 2550
<212> DNA
<213> 螺旋巴克斯霉（Backusella circina）
<400> 3
atgaagcctt tcggaccaat caagaccact ctttttatta ttattagcca cttctctttg 60
aattctttag ctgatacagt tcctactacc caggtagagg tcaaaactta tacttactcg 120
ggtggagagc tgtctggtac aatctacgta agtttctcgc tctaactttg gctttcttct 180
gtatatcaaa atcttattta aatacacttc cttcatatgt ttaggtcgag aacattgatt 240
atactaaggt tgttacggtc atttatgctg atggttcaga tgactggaat aacaatggca 300
ataccattgc tgccgccttc tctgaatcaa tctctgatac taattacgaa tactggactt 360
tctccagttc tgttaacagc attaaggagt tctatgttaa ggtgaatagt ttattcaata 420
cttttaatta tgattttacc atataaagat tatacatatg cttatcattc acttattaaa 480
tagtatgatg ttgatggaaa cacctactat gacaataatg gaaatgccaa ctaccaaggt 540
attttttttt atcaaactta atatttgtca atgttaacgc taacacatga tcatatagtc 600
tctgcttctt ccactaccac aactactgct acaaagacta caaccactgc aacaaaaacg 660
actgctactg ccacatctac tgccactggt tcaacttcat tcccttctgg taactccact 720
atctcttcct ggattaaggg tcaagaagat accagtcgat ctgttatgct tggcaacatt 780
aacccacctg gaagtgccac tggttttatc tctgcttctt tgtcaactag tggtcctgat 840
tattattatc attggacacg cgtaagtcaa ttccaatttg aacattatag aagttacctc 900
tctaatattc gaaaactaac ttttctcatt tatctatttt catcaggatg ctgctcttgt 960
tgctcacgtt attgtcaatg attataatac tactctttct ggagatagct ctactcttca 1020
agttattaag gattatgtta ctttctctgt taacagccag tctgaatcta ccgtatgcaa 1080
ctgccttggt gagcctaaat tcaatccgga tggatcaagc tacactggtg catggggcag 1140
gtaagatatt atagcattta ataaaatcat gcgcatgtta tattcattat taattttgta 1200
taacattaat cagacctcaa aacgatggtc ctgctgaacg tgccacaaca ttcattttct 1260
ttgctgacac ttatctcgca cagggtggtg actctagcta tgtgactggt acacttgccc 1320
caggtatata tagcctttta aaaaacaaat tactttaaaa agtgaatagt attactatgc 1380
taactacatt tatatattct ttactatttt gatttaaata gctatctatg ctgatctgga 1440
ttatgttgta aataactgga gcactggatg ttacgatttg tggtaaagca tctaatatga 1500
agttaatttt tgtgaagtat actcaccaaa agctgatata gggaggaagt taatggtatt 1560
catttctaca ctttgatggt tatgcgcagg ggtcttattg acggtgccag ctttgcctct 1620
cgtaatggtg actctactcg ttcaagcagc tatacttcca ctgctaaatc aattgctacc 1680
aagattgata gtttctggtc agcctccaac aactatgttg ctgtcagtca aagtgtaact 1740
tctggtgtca gtaaggctgg atatgatgct tctaccatta ttgctgctaa ccaagctagt 1800
cttggtgatg gtttctatac tcctggttca gataaggtag gctaatctta atgcaatatt 1860
ataaacgtag aaaaagaaac atgtatatta atgccaattg agtagatgct tgccacctcc 1920
gtcgctgttg agaatgcttt ctcttctctt tacaccgtta atactggaaa ggcttcttac 1980
ttaggaaatg ctattggcag gtgagtgcat ttgacacttc attttcttcg tataatttct 2040
cactaaaaaa actactacca tattaaaaga taccccgaag atacctataa tggtaatggc 2100
aactctcaag gcaacccttg gtatttgtaa gtaaaactct tttatgctcg ataattatcc 2160
cttcctaaca catcattttt atttttaaaa aaaaggtgta ctaacgctct tggtgagtta 2220
tactaccgtg caattaagga atggaatgcc gctggatccg ttacggtcaa cagtgtcaat 2280
ctcggcttct tccaaaagat tgattcctca atttcatctg gcaccacctt taccttaggc 2340
acttctgact acagcaccct cgttgacaat gttgcacttg ctgctgataa gttcttcgcc 2400
gttgtccagt atcatgaacg cagtaatgga tctatgcctg agcaatttgg ccgtgaggac 2460
ggtcttccta ctggtgctag agatcttaca tggtctcatg ctgccatgat ttctgctgcc 2520
cgtgctaagg ctggtactcc tgtgtattag 2550
<210> 4
<211> 2256
<212> DNA
<213> Benjaminiella poitrasii
<400> 4
atgaagtttt ctcatctctt caggaagcca acgcttctta ttgtgacctt tttgaccgct 60
acggtttttg cagaaactgt tcctacaact tctcaagtca agcttaagag ttatacatat 120
gatggttcca ccttatccgg ccagatttat gtaagttata ttaattattc actttatatt 180
ttttttttcc cctgacctgt caaaaatata ctaattttta tttcaaattt taccagattc 240
aaaatattgc ttatacaaag gtggttactg taatctactc tgatgcgtct agcaactgga 300
acaacaatgg aaacacaatt gctgcttcat atactaatgg tatctcagga accaattatg 360
aatactggaa tttcactggt cctgtcagca gcatcaatgc attttacatc aaagtaaatt 420
actgaataat aatgttcaca agcaattata aattctcact ttaattgatt atatagtatg 480
atgttagcgg aaatacatat tatgataaca acaataatgc aaactatcaa ggtaaaatag 540
tcaagagaaa aaaaaaaaga cgcattaacc gaactcaatt tattaatgca ttcttataat 600
agttacaaag acatcaacta caacgactac ttccactgct actgctacta ctactaccaa 660
aacttccaca ccgacttcta ctggtgtccc ctccaatttc cccaccggta acagtactat 720
tagcacatgg ttgaaatcac aaattggcat cagtcgctat gctatgctcc gtaacatcaa 780
tcctgctggt tccgctgttg gttttattgc tgcctcttta tcgactgcta accctgacta 840
ctattatgcc tggactcgtg attctgcttt gacctcatat gttattgcca acgattataa 900
ctctactttg gctggtaact caacaatcct tcaaatcatg aaagattacg ttactttctc 960
tgttaagagt caatcggttt ctactgcctg taactgcctt ggtgaaccca aattcaatcc 1020
tgatggatct agttacactg gcgcttgggg aaggtatgta gataatacca tacctttttt 1080
ttttttttca taatgatata catgtaaaaa gtttaactaa catgatcaat atgtaaatac 1140
aaagacccca aaatgatggt cctgctgagc gtgctactac atttatatta tttgccgata 1200
gttatcttaa gcaaacaggc gatgctagct atgttactgg aacccttaag ccaggtaaag 1260
ttttttcctt tattacggca tgtatattat gttaacatat acacgtaatt tgaatagcta 1320
ttttcaagga tttggactat gttgtcaaca catggactaa tggatgcttt gatttatggg 1380
aagaggttaa cggtgttcac ttctatacct tgatggtaat gcgtaagggt ttgatcagag 1440
gagctaactt tgccacccgc aacggcgact ctacacgtgc ttctacttac accaacacag 1500
ctgcatccat caaaacaaag atggattcat tctggtcatc tggcaacaac tacattgctg 1560
ttagtcaaag tgttactggt ggtgtcagta aggctggtta cgatgttgct aacattattg 1620
ctgctaatgt tggcagtctc caagatggcg tatatactcc tggttctgat agagtatgta 1680
tcaacagtaa ctttatagta tcaacagaga caatcatatt aaccattgca cccccccttt 1740
tttttctaga ttcttgctac tgctgttgct gttgaagcta aatttgcttc tttatatggt 1800
gttaactcaa acttaccagg ctaccttggt aactccatcg gtcgttatcc cgaagacaca 1860
tataacggta acggaaactc ccaaggtaac ccatggttct tggccacaaa tgcttacgca 1920
gaactttact accgtgccat cagagaatgg tatgacaatg gtggtgttac agtcaacagt 1980
gttaacttgc cgttcttcaa gaaattcgat agttccgctg cttctggtac aacctacact 2040
gtaggtacga ccgccttcaa cacgatggtt tccaatgttg cagctgcagc tgataagttt 2100
ttctcgactg tcaaattcca tgcttacacc aatggctcca tgagcgaaca attcggtcgt 2160
aatgatggtt tatgtacggg ggctcgtgat cttacatggt ctcacgcttc tttgatttct 2220
gctgctcttg caaaggctgg tactccttca gtctaa 2256
<210> 5
<211> 1968
<212> DNA
<213> 瓜笄霉（Choanephora cucurbitarum）
<400> 5
atggtttctt ttaacttcct taaaaagcca gtattcattg tgattacttg cttgacagtc 60
tctgtctgtg ctcaatctgt gcctaccagt gaccctgtca aggtcaagac tttcagctac 120
gatggtaact ctttctctgg tcaaatttat gtaagtgtca atacttattt ggaaggtacg 180
ggggtttaat tgtttaattg gatttcgcag atcaaaaata ttgcttatga aaagaccgtt 240
actgtcatct actctgatgg tactggtaat tggaacaaca acaacaacaa gatcgctgct 300
gctttctctg aagccatctc tggttccaac tatgaatact ggactttctc tgcctctgtc 360
cctagcatta aacaatttta tgtcaaggta aagaaacaat agttttaaac ataccaatct 420
aatgtgcctt catagtatga tgtttctggt aaaacttatt acgacaacaa tggtagcaag 480
gactataatg gtaaaatcaa attttcacat gaattaagag tatgttacac taaacctttt 540
ttttttttaa tagttgttac ctctggtcct accaccactt ccagtggtcc ctcaaagaca 600
accaccaatg gtcccgctcc tacctccacc aacactaact tcccctccgg taacccttcc 660
atcacttcat ggatcgataa gcaaattgac atcagtcgct ctgccatgct taagaacatc 720
aaccctgccg gtaccgtcaa gggtttcatt gctgcctctc tctctacctc aaaccccgat 780
tacttctacg cctggacccg tgacgctgcc ttagttgccc atgttgttgc taacgactac 840
aaccgtacca agagcggtga tgccacttac ctcggcctcc tcaaggatta cgttaccttc 900
tctatcaaca gtcaaaacac gcctactgcc tgtaactgtc tcggtgaacc caagttcaac 960
aaggatggtt ctggttacaa cggtccctgg ggtcgtcctc aaaacgatgg tcctgctgaa 1020
cgtgctgaca ctttcgtctt gattgccgac agtatcttga cccaaaccaa ggacgtcagc 1080
tatgtcactg gtaccttgcg ccctgctatc tacactgatc ttgactatgt cgtcagaacc 1140
tggtccaacg gctgtttcga tctctgggaa gaagtcaacg gtgttcactt ctacaccttg 1200
atggtcatgc gtcgtgcctt gcttgtcggt gccaactttg cctctcgtaa cggtgacagt 1260
gctcgtgcca gtaactacaa caacgctgct aactccatca agtccaagat cgacagcttc 1320
tggtcttcca acaacaacta tgttgccgtc agccaaagtg tcactggtgg tgtcagcaag 1380
gccggttacg atgtctctac cttgattgct gccaatgtcg gtagtctctc tgacggtttc 1440
tacacccctg gttctgaaag aatgcttgct actgccgttg ctatcgaaga caagtttgcc 1500
aacctctatg gtatcaaccg taacttggac agcagccttg gtaacgctat cggtcgttac 1560
cctgaagaca cctacaacgg taacggtaac tctcaaggta acccctggtt cattgctacc 1620
aacgcctatg ctgaactcta ctaccgtgcc atcaaggaat ggaacaacaa cggtggtgtg 1680
actgtcacca acgtcaacct caacttcttc aagaagttcg atggtagtgc ctctgtcggt 1740
accaagtaca ctgctggttc tgctgcctac aacaccttga ctcaaaacat tgctcttgct 1800
gctgataagt tcttcaacac cgtcaaggtc catgctgcca ccaacggttc catgtctgaa 1860
caataccacc gtgacaccgg ttccatgaca ggtgctcgtg acttgacttg gtctcatgcc 1920
agtttgatta ctgctgccct tgccaaggct ggtactcccg tagcttaa 1968
<210> 6
<211> 2204
<212> DNA
<213> 匍枝根霉（Rhizopus stolonifer）
<220>
<221> 尚未归类的特征
<222> (171)..(190)
<223> 任何核苷酸
<400> 6
atgaaactgt ttggtttgcc attgaagcct tcgctctttt ttgtggtctc ttatctttct 60
ttcctcgcct ctgctgctag tattcctagt agttctgaag ttcagcttga cacatacgcg 120
tatgatggtg ctaaattttc aggaaaaatt tatgtgagtt tttttttttt nnnnnnnnnn 180
nnnnnnnnnn tttttttttt tttttttttt ttcttctaaa ggaaggtatc tgatctcaaa 240
taaatttagg tcaaaaacat tgcttatgaa aagactgtca ctgtggtata cgctgatggt 300
tctgataact ggaataacaa tggaaatata atttctgcta ctttctctac cccaatctct 360
ggatctaact acgaatactg gaccttttct tcttctatta gtggtattaa agagttttac 420
attaaggtat agttcatttt ctttatatgt ataatttacc aaccttactt tttacagtat 480
gtggttagtg gtaaaaccta ttacgataac aacggcacta aaaactatca agtttctacc 540
acttcctcta ctaccactgc ttccaccaca accgctactc gtacaactac agctggaact 600
acttcgacct ctactacggc tgcacctaca agcacttcat ccggttcttt cccttcaggt 660
aactcgacgg tatcctcttg gattaaacgt caagaaaaaa taagtcgctt cgctatgctt 720
cgtaatatca accctcccgg aagcgctgct ggttttatcg ctgcttctct tagtacctct 780
ggccctgact actactactc atggactcgc gactctgcct taacttccaa cttgattgcc 840
tatgaatata acaccacttt gtctggaaac accactatcc ttaacatcct taaggattac 900
gtcaagttct ctatcagctc tcaaacaact tccactgtat gtaattgtct tggtgaaccc 960
aagttcaacc atgatggctc aagttacaca ggcgcttggg gaaggtagtt acacatattt 1020
ttttctggat gagaacacct gtttattaat attaacttag acctcaaaat gacggacctg 1080
ctgaacgtgc aaatactttt atcttgttcg ctgacagtta ccttgatcaa acaaaggatg 1140
catcttatgt cactggcact cttaaacctg gtaatttttt tgaattttat ttattgtata 1200
gatatgtgta cttatagttt cttaaagcca tttttaagga tttggactac gttgtcaatg 1260
tatggtctaa cggttgttac gatttatggt aaaaaaaaat atgatgtttc tttataggat 1320
cttattgaca gtagaaaata gggaagaagt caacggagtt catttttaca cattgatggt 1380
tatgcgcaag ggcttacttc ttggtgctga ctttgccaag cgtaacggtg actctacacg 1440
tgcatctact tacaccaata ctgcttctac tattgctaca aagatttcta gcttctgggt 1500
atcctctagc aactggattc aagtcagtca aagtgttact gctggcgtta gcaaaaaggg 1560
attggacgta tcaaccttat tggctgctaa tcttggaagt gttgaagacg gtttctttac 1620
cccaggttct gaaaaggtaa ttgtagttta tcatggataa gagtatggtt ttcgagctaa 1680
aatgcacatt taaatagatc cttgctaccg ctgttgctat tgaagatgct tttgcttcat 1740
tgtatcctat caactccaac ttaccttctt accttggtaa ttccatcgga agatatcctg 1800
aagacaccta caacggcaat ggaaattctc aaggcaaccc ttggttcttg gccgtcaatg 1860
gctttgctga gctttactac cgtgctatca aggaatgggt caacaatggt agtgttactg 1920
tgagcaacat cagcttgtct ttttttaaga aatttgactc atctgctact gctggtaaga 1980
cctatactgc cggtactgca gactttaaca atctcgcaca aaacattgca cttggcgccg 2040
atcgtttctt atcaactgtc cagactcatg cctttaacaa cggatctctt gctgaagaat 2100
atgatcgtac aactggagta tctacaggtg ctagagactt gacttggtct catgcctctt 2160
tgattactgc tgcttatgca aaagctggtt cacctgctgc ttaa 2204
<210> 7
<211> 2015
<212> DNA
<213> 卷枝毛霉（Mucor circinelloides）
<400> 7
atgaagttct ctaaccttct caagaagcct ctccttttga tcgctggtat tttggccgct 60
actgttgtcg ctgagactgt acctactacc gctgcagtca aagtcaagtc cttcacatat 120
gatggatcca ccttcgctgg tcaaatctac gtacgtacta aaatttcgat ttattgaatt 180
caatgctaaa cccgcattct tcattctata caggtcaaga acattgctta cactaaaact 240
gttaccgtga tttactctga cgcttctaat aattggaata acaatggtaa cacaatcgct 300
gcttcttaca gcgaaggcat ttcaggcacc aattacgaat actggacttt cagtgcccct 360
gtcagtggca tcaagcaatt ctacgtcaag gtaagtctgg gatcactcag tggtcggttg 420
caagtattga gcctataatc tattagtatg ttgtaagcgg taccacctac tatgacaaca 480
acaacagcgg aaactaccaa ggtatttaca ctttcacatg ttgcattagt attgtcatac 540
tgaaaaaaaa attccgtggc attcctttag ttaccactac taccactact actgctccta 600
cctctactac ttctggcggt tcttctacaa ccaccggcgg ctctactacc actgctacta 660
gcgtcccaac tggtgttcct tctggtttcc ctactggtaa ctcttctatt agctcttgga 720
tcgacggcca aacatctgtc agtcgttatg ccatgctccg caacatcaac cctgctggcg 780
cagtttctgg tttcattgct gcttccatgt ctacctctgg acctgattac ttttatgcct 840
ggactcgtga ttctgccctg acctctcatg ttgttgctta tgattacaac actactttgg 900
ctggtaactc taccattctc ggcctcttga agaactatgt tactttctct atcaacagtc 960
aaactacttc cactgtttgt aactgtctcg gtgagcccaa gttcaacaag gatggtagca 1020
gttacactgg tgcctggggt agacctcaaa atgatggccc tgctagtcgt gccgatactt 1080
tcattttaat tgctgacagt atcctcaagc aaaccggtga tgctacttat gttactggca 1140
ctcttgctcc tgccatctac aaggatttgg attatgttgt ctcaacttgg tccaacggtt 1200
gtttcgattt atgggaagaa gtcaatggcg ttcactttta cactttgatg gtgatgcgta 1260
gaggtttgat caagggtgct aactttgcct ctcgcaacgg tgacaacact cgtgccaaca 1320
cttacaccaa cactgccgcc tccatcaaga ccaagattga tagcttctgg aactccaatg 1380
gcaactatgt cactgtcagc caaagtgtca ctggcggtgt cagcaaggct ggttatgatg 1440
cctctgtgtt gattgctgcc aacttgggta gtgttcaaga tggtttctac actcctggtt 1500
ctgacaagat gcttgctact gctgttgcca ttgagagcaa gtttgcttcc ctctattcta 1560
tcaaccaaaa cttaaacggt taccttggta atgctatcgg ccgctatcct gaagatactt 1620
acaatggtaa tggtaactct caaggcaacc cttggttcat ctgtaccaat gcctttgctg 1680
agctctacta ccgtgctatc aaggaatggt ttaacaatgg tggtgttact gtcactagta 1740
tcagtttgaa cttcttcaag aaatttgatt cctctgctgc cgttggtacc aaatacactg 1800
ttggtacctc tgctttcaac agcttggtcc aaaatgttgc tgttgctgct gatgccttct 1860
tctccaccgt caagttccat gctgctacta acggctccat gagcgagcaa tatggtcgta 1920
gtgatggttt gatgaccggt gctcgtgatt tgacttggtc tcacgcttca ttgatttctg 1980
cttcttacgc aaaggctggt tcccctgctg cttaa 2015
<210> 8
<211> 2398
<212> DNA
<213> Dichotomocladium elegans
<400> 8
atgaaactta acagtgtttg gaaagcattc agtattttgc ttatggccac catggctttt 60
gctgccaccg ttcctaaaac gcaagtgaag cttgaggcgt acacatactc ggattccata 120
ttctctggta gaattttcgt aagaatctat atctcctcaa atcgtttgta tgcataaagg 180
ctcatatatt gtgaataggt gcaaaacatc gactacacca agattgtaac cgtttactgg 240
tctgatgcat cgaataaatg ggactccagc aaatattata ctgaagctgc ttacacccat 300
tcgatccctg gaactaatta cgaatactgg gatttctcag ccaccattgg tccttcagga 360
atcaagcagt tctacatttc ggtaaccatt tattgaggag aatcggtaaa tccacgcacc 420
tcttttactc atcgtgttcg gatgactttc agtatcaagt ccggggtgtc acttattatg 480
ataacaacgg cggctatggt gtcaattacg gtgagcaaaa cactgtcagc tagtagctct 540
tcatgcccta cttacaaatg cgttgtagac gtcatctcgt cacccccgac cacgtccgat 600
gccactacca ttcctactac taccactagt gctattcctt cttccactac cactattccc 660
gccagtactg gggttccttc tggaaactcc actatcaccg tttgggctga ttcgcagcat 720
aagatcagct ggaaagcaat gctcgccaat attaatccac ccggttctgc tactggtttt 780
attgccgcct cgctttcgac ttctggtcca gactactact atgcctggac tcgtgatgct 840
gcgatggttg cgcacgttat tgtcaacgct tacaacacaa ccaaagcagg tgatgcaact 900
actcttggcg ttctgaagga ctttgttacc ttccagatta aggctatggc taccagcacc 960
gtctgcaact gtctcggtga acctaaattc aaccctgtaa gttaccagat agtacagcag 1020
aaagaaaata ccgtctttac catcggtcgt aggatggctc cagctatact ggtccttggg 1080
ggagaccaca aaatgatgga cccgctgagc gtgctactac tttcattcta tttgcggaca 1140
gctatctttc ccagaccgga gacgccgctt atgtcactac cttgaaaaga ggtaagcatc 1200
ctggaagctt caagtgtgca gtgattaacc tgtaaattat gcttcttcca cttcattagc 1260
tatctttacc gatcttgact atgtggtgac cacctggcag gataattgtt ttgatctctg 1320
ggaagaagtg aacggacttc acatgtatac gtaagttgtt attcctcttt ttctcgtatg 1380
cacacgcata cccgcgcccg gagtattagc agaaaaaagc ttaagctact aatggaactc 1440
ttatttcagt ttggcggtaa tgcgccgaag tctggttgat ggagcaagct ttgccgcccg 1500
caatggtgac aatacacgcg ccagtaccta taccaatact gccaaatcca tcgagaccaa 1560
gcttgcaagc ttttacaact cgagtagcaa ctatgtcgtt gtcaccaaga acttcgcagg 1620
tggtgtaaac aaggctggtt tagatacctc cacattgatc gccgcgaaca ctgccggttt 1680
gggtgatggt aagcgggaag catgatgatt aatctgaccc ttagagaggg agaaaataga 1740
tagctctctt ttcaatatgt gattctgact gccagccaac ctctccccaa ggtttcttca 1800
cccccggttc accggagatt ctagctaccg ccgccgcaat cgagaagtca ttcgctgatc 1860
tatacggcat caacaaacaa attcccaact ggttaggcac tgcaattggc cgatacccgg 1920
aagacactta caatggcaac ggcaactcac agggtaaccc ggtaagtatc cctccaacct 1980
ttgtctctct cttcgttgtg ggacctataa tgcgatgctt atctatttaa cctagtggtt 2040
catctgtact gctaccttcg ccgaactcta ttatcgcgcc attctggaat ggcaacacgc 2100
tggctccgtc acagtgaaca acgtgaactt gccattcttc aaaaaattcg atagctccac 2160
ttcctctggc accacctaca ctgtcggcac tagtagcttt gattcactta tctcaaaagt 2220
agcctatgct gcggataact tcttttctac gatcaaatat cacgccgcta cgaatggttc 2280
catgagcgag cagttcaatc gtgataccgg ttttatgact ggcgctagag acttgacctg 2340
gtctcacgcg gcattcatca ccgctgctaa agccaaggct ggcactcctg tatactag 2398
<210> 9
<211> 2311
<212> DNA
<213> Fennellomyces属物种
<400> 9
atgagaccaa gtcttgtttg gaaaagcctg cttatggttg tgcttaccgc agccatgggc 60
acatatgctc aaactgttcc atctgtgcct atcaagttgg aaagttacac ttactcggat 120
aacgtattcg ccggtcgaat ctttgtaagt agttcgataa tttctgtttg ttaaagagaa 180
aaaaaaactc accccttttt ttcgtcacgt agattcgaaa catcgcttat acaaaagtgg 240
tcaaggtttt ctggtctgat gaatctggca attggaacgg taacggaaac tatgttgatg 300
cctcgtattc ggggtctatt tccggtacca actatgaata ctgggaattt tcaacaacca 360
tcggatccgc cggtattagc caaagctatt tgagagtaaa gagtttgtcc tttttaagtg 420
tttcgcaagt ggggaagaga gactaaacag aatattttct ttttagtatg atgtttcagg 480
ttctacgtac tatgataaca atggaaatgc aaactatggt aacgtattaa ctagaagcgg 540
tcaaatttga aatcactcaa agccttcttt tacgtgtttc agatatcgag gaagactcca 600
cacctacgcc caccaccacc actaccagta ctacgacaat aaccactacc actaccagtg 660
gaacttcaac tcctacttct gtccccactg aagttcctgc tggcaatacc actgtcactg 720
actgggtgaa tgatcaattg gaaattagtt ggccatcgct tttaaagaat gtcaatcctt 780
ccggcgcagt aaccggtttc atcgctgctt cgctttccac aaatgatccc gattatttct 840
actgttggac gcgtgatgcc gctttggtcg cacgagtgat ggtttacatg tacaacacaa 900
ccgaagctgg tgacaccagt ttgagaagca agctgcaaga ttatgttaca ttccagatca 960
actccatgaa gactgcaaca gtatgcaact gcttgggtga acccaaattc aataaggtta 1020
gtagtttttt ttttaaatga tgatacactt ttgacaaaaa agaattagga tggttcgggt 1080
tactcgggag catggggtcg cccacaaaat gatggcccgg ctgatcgtgc tatcacgttg 1140
atcctgtttg cggacagttt cattgctcaa ggcggtgatg tctcgtacat taccaacaca 1200
ttgaagccag gtaagtaatc gggtatcacc gtaatccggt ctccaattac taatgtatgc 1260
cacagctatc tacaccaatc tcgactatgt tgttaacacc tggagcaatg tttgctttga 1320
tctatgggaa gaagtcaatg gtgtccatat ctatacgtag gttttttcgg gggagaggga 1380
aacgttaatg tgaaactttt tttttgaagt actcattgaa aatgcttatt tagactttcg 1440
gtcatgcgta agggtttgct tgaaggtgcc gactttgctt ctcgaaacgg cgattccacg 1500
cgtgccaaca cttatagaag caccgcttct tccatcaaga cccgtcttga atctttctgg 1560
tcatcgagca ataattacat cactgttact caatcatact ctggtggtgt gaacaaggcc 1620
ggtttggatg tatccacctt acttgcagcc aacggagcaa gcatgaatga tggttagttt 1680
ttgcttaaaa aaattgactt tgatatttac caaaagaata ctgcgtaggc ttctatactc 1740
ctggctctga caagatgttg gcaaccgctg ttgctattga aaattcgttt gccggtattt 1800
acgctgttaa ccagaatcga cctgattgga aaggtactgc tattggtcga tatcccgaag 1860
atacttatga tggccatggc aattcccaag gcaaccctgt aagtcatata ttccttatca 1920
caagaatgaa ttctaactta gaaaatagtg gttcattgcc acagctgcgt atgctgaaat 1980
gtactaccgt gccatcttgg aatggcaaca gcaaccgtct atcactgtga actcgatcaa 2040
cctgagcttt ttcaaaaagt ttgactcgtc cgccgctgtt ggtactgtgt acaagcccgg 2100
tacccaagct ttcaacaaca tggtttccaa tgttgcgttc gctgctgatg agttcttttc 2160
tactatgaat ttccattccg ccaccaacgg atccatgagc gaacaataca accgcaatac 2220
tggtattatg caaggtgctc gtgatcttac ttggtcccat gctgccttca tcaccgctgc 2280
caaagctaag cttggcacgc cagtattcta g 2311
<210> 10
<211> 1985
<212> DNA
<213> 桃吉尔霉（Gilbertella persicaria）
<400> 10
atggttttct ccaagctttt taagaagcct atccttctca ttgtcactta cttgactgtt 60
actgttgtag ctgaaactgt tcctacttca gctcaagttc aagttaagtc cttcaactat 120
gatggctcta ctctttcagg tcaaatttat gtaagtaatt gagaggaagc gaatagtcat 180
tctgaatttg ctttaggttc aaaacattgc ttacgaaaag actgttactg tcgtttactc 240
tgatggctct gataactgga ataacaatgg taacaccatt gctgccagct attcttcttc 300
tatttcaggt agcaattacg aatactggac tttctcttct tctgttccta gcatcaagca 360
attctatatc aaggtataaa aaaaaaagga aacatgtatg acaaattagc tttactaaca 420
tcatcatctg tattcagtat gtcgttgctg gcaagactta ctatgataac aatggtacaa 480
agaactatca aggtatgtaa ttcacatgtt gcatgaaata tcagttacac taacacaaag 540
gctttaattg tatcaattta gtgagtgcaa gcacccctac taccactact accactactt 600
cttctggagc caccaagact actactacca ttggccctac ttctacaagt actgtcttcc 660
cctctggcaa ctctaccatt tcttcttggc tcaagggtca aattgaaacc agtcgttttg 720
ccatgctccg taacatcaac cctgctggta ctgtcaaggg tttcattgct gcttctttgt 780
ctactgctaa ccctgactat ttctatgcct ggacccgtga tgctgcttta gtcggccatg 840
ttattgccaa tgactacaac cgtaccttag ctggtaacag cacttacctt ggtcttttga 900
aggactatgt tactttctct gtcaactctc aatctacttc tactatctgt aactgtctcg 960
gtgaacccaa gtttaacaag gatggttctg gctacagcgg tgcatggggc cgtcctcaaa 1020
atgatggccc tgctgaacgt gctgatacat tcatcttgat tgctgacagt atcttgaaac 1080
aaactggtga tgctacctat gtaactggta ccttgcgtcc tgctatctac aaggatttgg 1140
actacgttgt caatgtttgg tctaacggtt gtttcgattt gtgggaagaa gttaatggtg 1200
ttcactttta cactttgatg gtcatgcgtc gttctttgat tttgggtgcc aactttgctt 1260
ctcgtaacgg tgattctact cgtgccagta cttacaccaa cactgccaac tctatcaaga 1320
ccaagattga caccttctgg tcttcttcca acaactatgt tgctgtcagt caaagcgtca 1380
ctggtggtgt caacaaggct ggttatgatg ttgctaactt gattgctgct aatgttggta 1440
gtctcgatga cggtttctac actcctggtt ccgaaagaat tctcgctact gctgttgctg 1500
ttgaaagtaa atttgcttct atttacggtg ttaaccaaaa cttgcccagc tggctcggta 1560
actctatcgg tagataccct gaagacactt acaatggtaa cggtaactct caaggtaacc 1620
cttggttcat tgccactaat acttatgctg agctctacta ccgtgctatc aaggaatgga 1680
ccaacaacgg tggtgtcacc gtcactaatg ttaacttcaa cttcttcaag aagtttgata 1740
gcagtgcttc tgttggtacc aagtacactg ttggtacctc tgcctttaac accttgactc 1800
aaaatgttgc tcttgctgct gacaatttct tctctactgt taaggttcat gctgccacca 1860
atggttctat gtctgaacaa ttcggtcgtg atagcggtgt catgactggt gctcgcgatt 1920
tgacttggtc tcacgccagt ttgattactg ctgctcttgc caagacaggt gctcctgtag 1980
cttaa 1985
<210> 11
<211> 2018
<212> DNA
<213> 卷枝毛霉（Mucor circinelloides）
<400> 11
atgaagttct caaatcttct caagaagcct ctccttctga tcgctggtat cttggctgtc 60
actgtagtcg ctgaaactgt ccctactact gaagcagtca aagtcaagtc ctttacctat 120
gatggatcta ccttggctgg tcaaatctat gtaagtgcta gaatcagtac caatcccttc 180
aataaacaca atgctaaata catgctctca acgaatagat caagaacatt gcttacacca 240
agacggttac tgtaatctac tctgatgcct ctgataattg gaacaacaat ggtaacacga 300
tcgctgcttc ttacagtgca gccatcgcag gcaccaacta tgaatactgg actttcagtg 360
ctcctgtaag cggcatcaag caattctacg tcaaggtaag tttgggattc ctgtatgctg 420
ggtgctcaga tattaagcct ctgatccgta ttagtatgtt gtcagcggta ctacctacta 480
tgacaacaac aacagcggaa actaccaagg tatattgatt ttcacatgct ggcatttgca 540
ctgtcatact aaacgagctg aatcctctag tttcagtcac caccactacc actactgctc 600
ctaccactac cacttctggc ggttcatcca ctaccactgg cggctccact actactgcta 660
ctagcgtccc cactggtgtt ccttctggtt tccctacagg taactctacc attagctctt 720
ggatcgatgg tcaaacctct gtcagccgtt atgccatgct ccgcaacatc aaccctgctg 780
gtgccgtcac tggtttcatt gctgcctcta tgtctacttc tggtcctgac tacttttatg 840
cctggacgcg tgattctgcc ttgacctctc atgtcgttgc ctacgactac aataccactt 900
tggccggtaa ctctaccatt ctcggtctct tgaagaacta tgttaccttc tctctcaaca 960
gccaaactac ctccactgtc tgtaactgtc tcggtgagcc caagttcaac aaagatggca 1020
gcggttacag cggcgcctgg ggtagacctc aaaatgacgg acctgccagc cgtgccgaca 1080
ctttcatctt gattgccgac agtatcctca agcaaaccgg cgatgctacc tatgttactg 1140
gtaccctcgc tcctgccatc tacaaggatt tggattacgt tgtctctact tggtccaacg 1200
gctgtttcga tttgtgggag gaggtcaacg gtgttcactt ttacactttg atggtgatgc 1260
gcagaggttt ggttaagggt gctaactttg cttctcgcaa tggtgacagc acacgtgcca 1320
ccacttacac caacacagct gcttccatca agaccaagat tgacagcttc tggaactcca 1380
acggtcaata tgtctctgtc agccaaagcg tcactggtgg cgtcagcaag gctggttatg 1440
atgcctctgt cttgattgct tccaacttgg gtagtcttca agatggtttc tatactcctg 1500
gctctgacaa gatgcttgct actgctgttg ccattgagag caagtttgct tctctctact 1560
ctatcaacca aaacttgaac ggttaccttg gtaatgctat cggtcgctac cctgaagaca 1620
cctacaacgg caacggtaac tctcaaggta acccttggtt catctgtacc aacgcctttg 1680
ccgagctcta ctaccgcgct atcaaggaat ggttcaacaa tggcggtgtc actgtcacta 1740
gcatcagttt gaacttcttc aagaagtttg attcctctgc tgctgttggc accaagtaca 1800
ctgtcggcac ctcttctttc aacagcttgg ttcaaaatgt tgctgtcgct gctgatgcct 1860
tcttctctac tatcaagttc catgctgcca ccaacggttc catgagcgag caatacggcc 1920
gcactgatgg cttgatgact ggtgctcgtg atttgacttg gtctcacgct tcattgatct 1980
ctgcttctta tgcaaaggct ggttctcctg ctgcctaa 2018
<210> 12
<211> 2946
<212> DNA
<213> 伞形卷霉（Circinella umbellata）
<400> 12
atgatgccga atcatatttg gaagtgtctg tttctagcca tgaccacagt tttcatgact 60
gtactggtac aaggtgctcc tacttcagaa attgaattgg actcttatac ctacaccggt 120
ggagtatttt ctggtcggtt atatgtatgt ggtatctatt tgtacataat taaaagaaaa 180
attcaagaga atacagtttt gttaatgttg gtaattccca atatatacag gttaaaaata 240
ttgcgtacac caaggaagtg aatgtctatt ggtctgatgc ttctgaagac tgggccaata 300
atggtaatta tgtggcagct acgtattctg aagccatttc gggtacaaat tacgaatatt 360
gggaattctc tgccaaaatt ggatcttctg gtattagtga aagttacata aaggtaaatt 420
actataaatt gtttgaaact ccaaaataca aaataaaaaa tccggcatag ttattaatta 480
ttctttattt ttttcaaata aattcagtac actgtgtctg gctccactta ttatgataat 540
aatgggggta aaaattatgg tatggtcaaa acattttaca cagtaataac gcctttcttg 600
catcatcgaa cttttaaagt atatgcttat ttttttttcg tcattttaca atagctatta 660
ctgagacaac tacacccaca ataactgcaa ccccgacatc aacaacggcg acaacgacca 720
tttcttcaac aactactacg gctacttcca ttcctacctc tgttccttcc actgttcctg 780
agggaaatgt tactgtcaca gaatgggtca ataagcaact aaaaatcagc tggtccgatc 840
ttctgcaaaa tgtaggcgta taataactaa aaatacctta tattatattc tatgcatact 900
atctgtaaaa aaaaccttaa aaattattga aaacctgttt ctcattattt atattacttc 960
tatcttatat ataggttaac ccttcaggta cagttactgg ttttattgct gcaagtttaa 1020
gtaccagcaa ccctgattac ttttactgtt ggacaagaga tgctgccatg gttgcacgtg 1080
ttatgactta tatgtataat actactgaag ctggcgactc aagtcttgaa agtgcattaa 1140
aagattatat tacgttccaa atcaattcaa tgaagactgc cactgcatgt aactgcttag 1200
gtgagcccaa gtttaatact gtaagttacg tattagagta agaaataaaa ccccctcgat 1260
tgatcactag acatgttatc tgtatcacgt atcattacaa tgctaatttt ttatttattg 1320
acaaatgtgg tactgtagga tggaagtggt tatagtggcc cttggggtag gcctcaaaat 1380
gatgggcctg gtaagtttaa actatttttt ctattttatt ttactttata agatttttaa 1440
aaatatgttg atcttatgat gatcttatgc cttaacaaaa atgttttttc atatttatac 1500
atatagctga acgtgccact acaatgattt tgtttgctga tagttatctc gctcaagggg 1560
gtgatacttc ctacgttaca aatacattaa aacctggtaa atcattataa taatattttg 1620
tatactataa caaacacaga tagatgtaaa aaaaggcttt ctgtttctca tgtatattaa 1680
caaaaacaat ttaccttttt tttttgttat taaaacgatt atatagctat ctataccaat 1740
cttgactatg ttgttggtac ctggtcaaat aattgtttcg atttatggga agaagttaat 1800
ggtgttcata tttgtaagca atatcatgat atttttctaa aaagaacaat acacacacat 1860
acacacacac acacacacac acacacacac acacatatat atatatatat atatatatta 1920
actgctatca acatttttac ttcataaaaa ctaaaataac aataataata ataatagtta 1980
ctctggctgt tatgcgcaag tcattacttg atggtgctga ttttgctgca cgtaatggcg 2040
atacatctcg tgtcagtggt tatcaaagca cagcttcatc tatcaagact aagttagaat 2100
cattctggtc gtccagtaac aattatatca ctgtaactca atcctattct ggaggtgttc 2160
aaaaggccgg attagatgtt tctactttaa ttgctgccaa ccaaggaagt atgggtgatg 2220
gtaagttttt atttttattt tactataata aagtagtttg taatataaaa tacgtaaaaa 2280
agaaaagacg gattttttta tatgaaatat aactattgct ctgtaggatt ctatactcct 2340
ggctctgata aaatgctggc tactgccgtc gctattgaaa actcatttgc aaatatttat 2400
actatcaata aaaacaaacc ttcttggctt ggtactgcta tcgggcgtta tcctgaagac 2460
acatataatg gtaacggcaa tggtcaaggc aacccggtaa gtttatatat aacccattta 2520
ctaaaagaag gtttcatttt atatatgtat ctaatatatt atatggtcac tactattatt 2580
tagtggttta tcgcaacagc aacctatgcc gaattatact accgagctat tttggaatgg 2640
caacaacaag agtctgttac tgtaaactct gtcaactttg atttctttag caagtttgat 2700
tcatcggcta aggttggcac tgtttataca ccaggtacag atacctttaa caccatggtt 2760
tcaaacgtag cttttgccgc tgatgaattc ctttcaacaa tggaacatta tgctgctaca 2820
aacgggtcca tgtctgaaca atttaatcga gagactggct ctctcaccgg agcccgtgat 2880
ttgacatggt cccatgccgc atttatcaca gctggtaaag ctaaattagg tattccatca 2940
ttctga 2946
<210> 13
<211> 2054
<212> DNA
<213> Mucor cordense
<400> 13
atgaagtttt ccaagctctt tacgaagcca actctcttca tagttagcat catcatttct 60
gttgcttcct ctgaaactgt ccctactact gccaatgtcc ttgtcaaatc ttacacttgg 120
gacggtgcca ctttatctgg tcaaatttac gtaagtatat tatgtaatgg cgtgttatag 180
caaaaattga cactgcttgt ctatagatta aaaatcttgc ctatgccaag gttgtctctg 240
tcatttactc tgatgctaat gataactgga ataacaatgg taacaaagta gctgcctcct 300
actctgctgg aattgatgga acaaactacg aatactggac cttttctggc gctgttagcg 360
gtatcaagca attttatgtc aaagtaagta tagatcttct atattcaaat tgaatatata 420
aactaactgt ataatgtagt atgatgttag cggtactagt tactatgata acaatggtac 480
caagaactac caaggtaaaa attagataat gaagtaatcg tatacagatc cttacatgtc 540
gtcttcatag ttaccaagac ctctacaacc accactgctg ctactaccac taccactgcc 600
actactacta ctggtggtac tggtaccact actacaacta ccgctactgc cactgctaca 660
tctactgatt tccctacagg taacagcact atcaccactt ggctcaaatc tcaagaagac 720
atcagtcgtg gtgctatgct ccgcaacatc aaccctcctg gtgccgctac aggtttcatc 780
gccgcctcat tatccacttc cggacctgac tactactatg cctggactcg tgattccgcc 840
ttaacctccc acgtcattgc tcacgactac aacactacat tagccggtaa caccaccatt 900
ctcaacatcc tcaaggacta cgtcactttc tctgtcaaga gtcaatctgt ttccaccgtc 960
tgtaactgtc tcggtgaacc caagttcaac cctgacggct cttcttacac cggcgcttgg 1020
ggtcgtccac aaaacgacgg tcctgctgaa cgtgcttcca cattcatcct ctttggtgac 1080
agttacctca agcaaactgg cgatgctact tacgtcacgg gcacattggc tcctgctatc 1140
tacaaggatt tggactatgt cgtgaatact tggtcaaatg gttgctttga tttatgggaa 1200
gaagtcaatg gtgttcactt ttacaccttg atgagcatgc gtagaggttt gcttgatggt 1260
gctaatttcg ctaagcgtaa tggagatact acacgtgcca ctacttacac aaataccgct 1320
gcttctattg ctacaaagat cgataccttc tgggtctctt ctggtaacta tgttcaagtc 1380
agtcaaagtg tgactggtgg tgtaagcaag gctggttatg attgtgctaa tattctcgct 1440
gccaacatgg ccgccaacca cgatggtttc tatactcctg gttcaagcaa ggtaaatata 1500
catgactcac tatctcttta tgaaagtata ctaaacgttt catatagatt cttgctactg 1560
ctgttgctat tgaatccaag tttgcttcct tatacagcat taactctggt ttggctagct 1620
ggctcggtac agctattggt cgttatcctg aagacactta caacggtaat ggtaactctc 1680
aaggtaaccc ttggttcatc tgtaccaacg cctttgctga actctactac cgcgctatca 1740
aggaatggac tcaagctggt tccgtcactg ttgacagcac tagtcttaac ttctttaaga 1800
aattcgatag ctctgctgct gcaggcacga aatataccgt tggtacttct gctttcacta 1860
atttggttca aaatattgct aatggtgctg ataagttttt gtctacttcc aagttccatg 1920
ccgctaccaa tggttccatg tctgaacaat ataaccgtga ttctggtttg atgacgggtg 1980
ctcgtgattt gacttggtct catgcttctt tgatctctgc ttctagagct aaggctggct 2040
ctccttctct ttaa 2054
<210> 14
<211> 2709
<212> DNA
<213> Phascolomyces articulosus
<400> 14
atgggtgtat tagcagacgt ccctacctct gccaatattg aactggatta ttatacttat 60
aaggataaag tattctctgg tagaatttat gtatgaattt taaaatagag taaaatgaaa 120
gaaaaaaaag atagaagaat gggaatatat aataattaaa ggttataata tatattttat 180
taaataggtt aaaaacattg catatgagaa aacagtagct gtctattggt ccgatgcttc 240
tggtgattgg aataataacg gaaataatgt tgcagcttcg tactctgaat ctatttcagg 300
tactgactat gaatattggg atttctcaac cacaattgga tctggtggta tcaagcaaag 360
ttacttgaag gtacacttga ttgttatcat cctgttattt ttcaaattat aaatatatat 420
actaaaagta cacatactca atagtatact gtatctggta atacttacta tgataataat 480
ggaagcaata actatggtac gtctcaattt aaaaaccata taaacaagta tatttctgta 540
ttcgtattca ggaacgtatg aagctgacat ataagttttt gattatagat attaccgaga 600
ccactgctac tactactact accacaacaa caacaaccac tactggctcc accgcaacaa 660
catcaacaac ttctggtcct accagtacta ccagtaccac acccggccct acacctacca 720
atgttcctga tggcaatgtc actgttacag agtgggttaa cactcaattt gccatcagct 780
ggcctaccct cttgaaaaat gtatctatca aaaaaatata atccttctcc aaaatactca 840
catgttcatt acttatatat cgttttcttt ttaatactaa aggtcaaccc atctggtaca 900
gtgaaaggtt ttatagctgc aagtttaagt accaacaatc ccgattactt ttactcctgg 960
actcgtgaca gtgccttagt ggctcataca atgacatatc tttataatac atcggaagcc 1020
ggtgactcta ctatcgaaag tgcattgaag gattatgtta cattctctat caacgctatg 1080
aatgctgcta ctgtttgtaa ttgtttaggt gaaccgaagt tcaatactgt aagtataagg 1140
ggaaaaaaga gacgatactt taaaaatgat agacttatca ttggatgaaa cttttttttt 1200
aacaaatagg atggaagtgg ttatactgga gcatggggaa ggtaggtttt ttatttacat 1260
gctggctcca gtgcttaaat gatatgatta tttgggagtg aaaaataaat gttgtgagca 1320
accttgctaa acttgtttct attattatta gaccacaaaa tgatggccct gcttcaagag 1380
caacaaccat gattttgttt gctgatagtt tccttgctca aggaggtgat gtttcctatg 1440
tcatcaacac actgaagcca ggtatatact attatatgtc ccaactacat ttattgaaat 1500
ataaagtact aatcttaaca aatattatta tttatcttgc ccaatgatat ttagccatct 1560
ataaagatct tgactatgtt gttagtacct ggtccaatac ttgttatgat ttatgggaag 1620
aagtcaacgg tgttcatatc tgtaagtagt agtatatctc ttaaataatg catcattctg 1680
aatatacata tttatcttca attttttttt taaatagata cattatccgt tatgcgcaga 1740
gcactgattg atggagctaa ctttgctcaa cgtaatggtg atacttctcg cgtgagcggt 1800
tatactagta ctgcaaccac aatcaagaca cgtctcgaat ccttctggtc agatagcaat 1860
aattatatta ctgtcactca atcctattca ggtggtgttc aaaaggctgg attggatgtc 1920
tctactttga ttgcagccaa tatcggaagc gtgggtgatg gtatatttca tctattacta 1980
ctttatttcc atattattgc gttatcggat cgatcttctc tgtcatttta taacgtaata 2040
tttattaata tctcttttct aatatttata ataaggtttc tatactcctg gttcggataa 2100
agtcttggct actgctgttg ctattgaaaa atcatttgcc aatctttaca ccatcaacca 2160
aaacaagcct tcatggcttg gtaatgctat tggtcgttat cccgaagata cttatgatgg 2220
ttatggtaat tccaagggta atcccgtaag tatttttttt tatctcttcc gtttatactt 2280
ttaatgcaga taatgatgat atcatcacta tgtccggtcg aatagtaatc catttgttgt 2340
atatagtggt ttattgctac tgcaacttat gccgaattat actatcgtgc cattttggaa 2400
tggcaacaac aagcttctgt tactgtcaac tctatcaatc ttggcttctt tagcaagttt 2460
gattcctctg ctagtgtggg tacagtctat acacctggta cagatagctt tgccaacatg 2520
gtatccaatg tcgcctttgc tgccgacgaa ttcttgtcca ccattgacta tcatgccatg 2580
aacaatggat ccatgcatga acaatacaat agagatactg gtatctctca aggtgctcgt 2640
gatttgactt ggagtcatgc tgctttcatt acagctgcca aagccaaatt aggtgcccca 2700
gcattttaa 2709
<210> 15
<211> 2033
<212> DNA
<213> 小孢根霉（Rhizopus microsporus）
<400> 15
atgaagttaa tgaactcgtc gatgaagacc tgtgtcttct ttattctctc ctacttttct 60
ttgttggtct cttctgctgc agtccctact agtgctgctg tacaggttga atcttacaaa 120
tatgatggca ctaccttctc tggtcgtatc tttgtaaggc taattgatct catttaaaga 180
aaaagagaga aactgatctc gtcaattagg ttaaaaacat cgcctactca aaggtagtca 240
ctgtcattta ttctgatggc tctgataact ggaacaacaa caataacaag atttctgctg 300
cttactctga agctatctct ggttcaaatt acgaatactg gacattctct gccaagttga 360
gtggtatcaa gcaattctac gtcaaggtaa ttcttttttt ttctctctat ttattgtgta 420
tactaacgcg acctttgata gtatgaagta agtggctcta cctactatga caacaacggt 480
acgaagaatt atcaaggtaa gcatatgctt aagcaatagc atttctttac tgacctatca 540
aatagtccaa gcaacaagcg ctacctctac tactgctact gctactacta ctacatccac 600
cagcactact accactagca ctggcccaac aagcactgcc tccgtctctt tccccactgg 660
taactctact atctcatctt ggattaagaa ccaagaagaa attagccgtt ttgccatgct 720
tcgtaacatc aatcctcccg gaagtgccac aggatttatt gccgcttctt tatctaccgc 780
tggaccagac tactactatt cttggacccg tgattctgca ttgacagcca atgtgatcgc 840
atatgaatac aacaccacct tcgctggtaa caccaccctt ttgaaatacc tcaaggacta 900
cgtcacattc tctgtcaagt cccaatccgt ttctactgtc tgtaactgcc ttggtgaacc 960
caaattcaac gctgatggct ctagctacac tggtccatgg ggaaggcccc aaaacgatgg 1020
acctgctgag cgtgctgtta cttttatgct gattgctgac agctacctta ctcaaacaaa 1080
ggacgcttcc tatgttaccg gtaccttaaa gccagctatc tttaaggatt tggattatgt 1140
tgttagcgta tggtcaaatg gatgttatga cttgtgggaa gaggttaatg gtgttcattt 1200
ctacactttg atggttatgc gcaagggttt gattcttggt gctgattttg ctgctcgtaa 1260
tggtgactct tctcgtgctt ctacctacaa gaacactgct tccacaatgg aatctaagat 1320
atccagtttc tggagtgact ccaacaacta tgttcaagtc agtcaaagcg ttactgctgg 1380
tgtcagcaag aagggtttgg atgtttctac tctgctggct gcaaacattg gtagtcttcc 1440
agatggtttt ttcactcctg gttctgaaaa ggtatttgca aaaagtcgct caatatttca 1500
tagattgtaa acgtgatttt atctagatcc ttgctactgc tgtcgctctt gaaaatgcct 1560
ttgcttcatt gtatcccatc aactctaacc ttccctcata cctcggtaac tctattggaa 1620
gatatcctga agacacatac aacggtaatg gtaactctca aggtaaccca tggttcttag 1680
ctgtcaatgc ctacgctgag ctctactacc gtgccatcaa ggaatggatc agcaatggta 1740
aggttactgt gagcaacatc agcttgccct tctttaagaa attcgactcc tctgctactt 1800
ctggaaagac ttacactgct ggcacttctg actttaacaa ccttgctcaa aacattgcct 1860
tgggtgctga tcgcttcttg tccactgtca agttccatgc ctataacaat ggctctttgt 1920
ctgaggagta tgatcgctct actggtatgt ccactggtgc cagagatttg acttggtccc 1980
atgcctcttt gatcactgct gcttatgcaa aggctggctc tcctgctgct taa 2033
<210> 16
<211> 1469
<212> DNA
<213> 伞菌霉（Spinellus fusiger）
<400> 16
atgaagtcac cttatagcat gaaaaccgtt ttggcattgt tgtctcttac cccgcttttc 60
acgtcagatg ttgttgctgt gccttctggt aactccacca tcactgcctg gggaagcaag 120
caggacggca ttagtttctc caccatgctt ggtaacatca accctcctgg atctagcaag 180
ggattcattg ctgcctctct ctctactgct ggtcccaatt actactactc atggacccgt 240
gactctgctt tggtagctcg tgccatcacc tacaaataca gcacttctta ccagaacgac 300
cccaagattc tcggtctctt gaaagactat gttacctacc aggtgaacga acaaacagaa 360
agcaccgtct gcaactgtct cggtgagccc aagttcaacc ccgatggttc tagcttcagt 420
ggtccatggg gaagacccca gaatgatggt cctgctgaac gtgcctccac catgattctg 480
tttgccaaga gttactatgc tcagaccaat gatgttggct atgtcagcaa tacactgaag 540
cctgccattt acaaggactt ggattacatt gtgaatgtgt ggggtaacaa ctgctttgat 600
ctctgggaag aagtcaatgg tgtccacttc tataccttga tgatgatgcg ccatggtctt 660
gtccagggct ctatctttgc taacactctc ggtgattcta ccagagccaa cacctacaag 720
acagctgccc agaacatcaa gaacagaatt gatactttct gggaatctgg aagcaactac 780
attgtcgtca ctcagaacca gagtgcaggt gttaacaagc cctctggatt ggatgttgct 840
gttttgcttg ctgccaacca aggtggtctg ggagatggtg tctatactcc tggctctgac 900
aaggtaaatg attatctgct ggtcttttta gacacaccaa actcatccat tttatatgat 960
aggttcttgc tactgctgtt gctcttgaga aatcttttgc cagcttatac cctatcaaca 1020
agaaccttcc ttcttactac ggtactgcca ttggaagata ccccgaagac acctacaatg 1080
gcaatggcaa ctctgaggcc aacccatggt tcattgccac caccacttat gctgagttgt 1140
actaccgtgc tatcagtgaa tggaccaatg gctcaggtgt cactgtaaac agcatcaaca 1200
aggagttctt cagcaagttt gatgccagtg ccaccaacgg aaaggtctac acacctggat 1260
ccgatagctt caacagcctg gtcaacaacg ttgccattgc tgctgataac ttcctttcta 1320
ctgtgagata ccaccaaacc agcaatggct ctttatctga acagttcaac cgctacactg 1380
gattcatgac aggtgcccgt gatcttacct ggtcccatgc tgccatggtc actgctcttg 1440
ctgccaaggc cggtactcct tccccttaa 1469
<210> 17
<211> 2402
<212> DNA
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 17
atgcagctaa acctttactg gaaaagtctg gccgtgctcg ccctgattca gatggtcatg 60
gcggccactg tgccaaccac tcaggttcag ctagactact acacgtactc caacaatgta 120
ctgtcgggcc gcatctatgt aagccgagat agaccaggaa acgccgctga aagagtgctc 180
attacatata tatcttttct tgcaggtgca aaatattgca tactcaaagg tggtcaaagt 240
gatttactca gacgcgtctg gaaactggaa caacaacggc aatactatct ccgcaagcta 300
tgtcgagagt atctctggta ccaactatga gtattgggat ttctcggcca ccattggcac 360
ggctggtatc aagcaattct atcttcgcgt gagtttgtca cctgccttgt ctcttattat 420
taagccatta ggctcgagtg acattaatgc tgtatttcat gtgcatatta gtatgacgta 480
tcaggtagca cttactatga caacaatggt ggggataata acaactacag tacgtattat 540
cggacagcga gagtcactta gccacttatg taacatatta cacatataga cgtcgtcgcg 600
acgacttcaa caacctcgtc atccacaacc acgactacgg ctacaacgac aaccgccaca 660
aagactacta gcaccacaag tgccacagct acgcccacgt catctgcgac ttttcctagc 720
ggcaattcaa cgatcaccac ttgggcaaaa tctcaaaagg atattagctg gaagacactg 780
ctcaccagcc tgaatccctc cggcactgcc aaaggcttca ttgctgcatc gctttccaca 840
tcgaacccag actattatta tgcttggacc cgcgactcgg ccctggttgc caggttggtc 900
gactccattt attgcacttg ggcaagcacc cttcttacgc gacgaattga tggttagaac 960
catggtcaac atgtacaaca ccacggaagc tggaagtgcg tcggttctag gccttttgca 1020
agactatgtc accttccaaa ttaatgccat gagcacttct acggtatgca actgccttgg 1080
tgagcccaag ttcaacccgg atggctcgag ttacaccggt gcctggggcc ggtaattgca 1140
aaatcacgac ggtttgcgtt tcgtaatggt tgatttctgt gtttgtagtc cccagaacga 1200
tggccccgct gaacgtgcca gtaccttcat tctgtttgcc gatagttata tcgctcaagg 1260
tggccagctc tcatatgtga caggaactct cgctcctgct atctacaagg atctcaacta 1320
tgtcgtgagc acctggtcaa ataactgctt tgatctttgg tatgatattg gtcgatattg 1380
catatttccg tcacgtattt ctaactcgtt ctaatatagg gaggaagtca atggccgaca 1440
tatgttcacg taaggaagga tatgcgctaa cgttcctttc tcagatttga tctcatttct 1500
tgtagccttg ctgtcatgcg ccgtgctctc ttggatggtg tcaacttcgc ttcgcgcatc 1560
ggcgatacga cttactcgag cacgtggtcc agtaccgcat cgtctatcca gtcaactttg 1620
tccggctact atttgtcgag cggcaattac attactacgg tgcagaactt ccagtcaggc 1680
gtcagcaaag ccggttatga cgtctcgact ttgattgctg ctaatgtggc cggtatgggc 1740
gatggcttct ttacgcctgg ctcggacgag gtgaaccatc aaaccgcgta aacttcgtag 1800
tagaagactg accgtggtgt aggtactcgc cactgctgtg gctatcgaga acaaattctc 1860
cagcttgtac ggcgtcaacg cgaacaaagc ttcttatctc ggcactgcca tcggacgcta 1920
cccagaggat acctataatg gctatggtaa cggccaagga aacccggtaa gtattactgc 1980
acttttcgtt tgtactcaaa catgcagaac cgcgatctaa atggcaggtc cgtatgtagt 2040
ggtttattgc aacggcggca tatgctgagc tgtactatcg tgccattttg gagtggcaga 2100
ccaagtcctc catcgtagtc aactccaaaa acctcggttt cttctccaag tttgattcat 2160
ccgctgcagt gggtactacc tatacgcctg gcaccacagc ttacagcaac atggtgcaaa 2220
atgttgcctt ggctgccgac cggttcttgt cgaccgtaca gctccacgcc gctaccaatg 2280
ggtccatgag cgaacagttc aatcgtgata caggcgttat gcagggtgca cgggatttga 2340
cttggtctca ctctgctttc attaccgctg cgcgcgccaa gcttggttcg cccgtctact 2400
ag 2402
<210> 18
<211> 1827
<212> DNA
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 18
atgcgcgcta caacagctat tctgtctctc tttaccactg cgtccatggc gctggccgcc 60
aacagcactg tcccggatgg caactcaacc acgaccgcct gggtcaagaa gcaagaagcc 120
atctcttgga cagatctcaa gaccaatgtc aaccctgaag gtgcggccaa gggctttatt 180
gctgcgtctc tttccacatc ggagccagat tattactatg cttggacgcg tgattctgct 240
cttgttgcgc ggtatgctca acgtgacatt tacaatgcct aatactgaca taataatatt 300
cagcgtcatg gtcaacaagt acaacacgac cgatgcgggt gacgccaacc tgctaggtct 360
tttgcaagac tatgtgtcgt ttcagatcaa tgccatgggt gaaagcactg tctgcaactg 420
cttgggtgag cccaagttta acccggtaag aagacaaaaa acataagaca gaaaagaaca 480
atcggctgat tctgggacct tgttacacac aggatggctc tagctacact ggagcctggg 540
gccggtgcgt atttgattag atgctcctgt accacgttaa tgttaatatt tgcccacata 600
tagccctcag aacgatggcc ccgcggagcg tgcttcgact tttatcttgc ttgctgatag 660
catgattgct caaaagtcag cgaatggttc ttatgtctcg gataccttag cccctgccat 720
ttacaaggac ttggcctatg ttgcctccac ctgggagaat gcttgctacg atctttggta 780
aggctattcc aatgcaaatt atgtctgcta gcttcatttc taacgaaccg gttctttaat 840
cttttaggga agaagtcaat ggcaagcaca tgtacacgta agtactatat attctttgaa 900
tgattattag taaagtatat atatgactaa tgatgctttg tctttgtatc gctagcctgt 960
ccgtgatgcg ccgcgctctt ctagatggtg ctgattttgc tagccgccag ggtcagacgg 1020
cgaatgtgac ctcgtggaag tcaaccgccg acaagatcaa gtcttcgctc gagggtttct 1080
tctcatccga taatggttac attgaggtga cccaagatat gcagggcggt gtccaaaaga 1140
agggcctcga tgtgtcgacc ttgatcgcag ccaatattgg cagcatgggc gacggtaata 1200
tcttcttatg cgagctatat gcctgcgctc aacaacatta ttaggcttct acaccccggg 1260
ctcagacgag gtgctagcca ctgccgtggc tgtcgaggct gcgttcgctg acctctacaa 1320
gatcaatcag aacacgaact ccaccggcct tggtaccgcc atcggccggt accccgaaga 1380
cacttacaat ggtgtcggca acagccaagg caaccccgta agtcactatc atctttgttg 1440
attatctatt ttaacttcta ttagtggttc attgcaacca acacgtttgc tgaactctac 1500
tatcgcgcta ttcttgagtg gcagaacaag ggtacaatca cggtcaactc ggtcaacgcg 1560
gccttcttct ccaagtttga ttcctcggct aaggctggca ctacatacaa gtcaggcagc 1620
actgaatttg acagcttgat caataaggtg gctttggctg ccgatgcctt cttgaacaca 1680
gtccagacgt atgctgcaag caacggctcg atgagtgagc aatacaaccg tgacaccggt 1740
gcgctgacgg gtgctcgcga ccttacttgg tcacacgcca gcttgatcac tgctgcaaac 1800
gccaagctcg gtactcctta taactag 1827
<210> 19
<211> 1796
<212> DNA
<213> Thermomucor属物种
<400> 19
atgaagcttg gccgtatcgg attcagtatc ctgtcagttg cctccgtctt ttctcaagct 60
gttgttactg ctgctgctgc tgcttcggta ccctccggaa acgcgaccat cacgtcgtgg 120
atccaagagc aactggccat tagctggaac accatgctga cgagcgtcaa tccggttggc 180
gccgtgaccg gcttcattgc cgcgtcgtta tcgaccgcga acccggacta ctactactgc 240
tggacgcgcg acgctgcgct ggtggcacgc gtcatgacgt tcatgtacaa caccacccaa 300
gccggcgaca gtggcctttt gggaatcctg caagactatg tgtcgttcca gatccatgcc 360
atgggtgaat ccacggtttg caactgcctt ggtgagccca aatttaatcc ggtaaatgcg 420
cggccaatcc cccaaacttt ctatacgtat atatattggt tcaagctgaa cgatggttta 480
ggatggttcg agttacactg gcgcatgggg ccggccccag aacgatggac ctgccgagcg 540
ggcgtcgacg tttatcaaaa ttgcagatag ttaccttacc caaaccggtg acgtttcgta 600
cgtcacgaat acgctgaaac caggtgcgta tatatatata tatatatatg tatatatata 660
tagaacagga tacaacaaaa ggtagtagac gaagaactca actttttttc ggacaaagcg 720
atctatgagg atctggacta tatcgtgaat gtttggcaaa atacgtgctt tgatctgtgg 780
gaagaagtct atggcatgca tatgtacacg taagttgtat tctctctatt atgtccattc 840
ctgtggatct tcgtacttat gatgtatgtc catgtaaaga ttggccgtga tgcgccgcgg 900
gttactggac ggcgcggatt tcgcgacgcg caacggcgac accgacaaag ccagcaccta 960
caccagcacg gcaacgtcca tccagacccg gctcgcgacg ttctggtcgg atagcaatgg 1020
ctacattact gtgacgcaag attaccaaag tggggttagc aaagccggac ttgacgtctc 1080
gactctgatt gctgcgaatg tggccggcat gaacgatggg ttctttacgc cgggctcgga 1140
tgaggttcgt gttatacata cttgtacacc aaggaggatg cctattgaca aaaaaagcgc 1200
ggttgttcta gatacttgct acggctgtca aggtggaagc tgcgtttgca aacctttacg 1260
gtatcaacat caacaaggcc agttatcttg gtaatgcaat tggtcggtat cctgaagaca 1320
cgtataacgg caacggcaac tcggaaggca acccggtaag ctatcccatt tttgtccccc 1380
gatttctaag tttgcaactt tctctaacca atgttgcttg gtgttaaaag tggttcattg 1440
ccacagcggc gttcgcggag ctttactacc gggcgatcat ggagtggcaa ttgcgcggca 1500
gcagcatcac ggtgaactcg gtcaaccagg gcttcttcac caagtttgat ccgtcggcta 1560
cggctggcac gacgtatacg ccaggcacgg acgcgtttaa ctcgctgatt gacaatgtgg 1620
cgctggcagc cgatcagttc ttctcgacca tccacctgca ccgtgcgacg aacggctcca 1680
tgagtgagca gtataaccgc gatacggggt tcatgcaagg cgcgcgggat ctgacttggt 1740
cccatgcggc gtttatcacg gccgccaagg cgaagcaggg caccccgtca ttctag 1796
<210> 20
<211> 2883
<212> DNA
<213> Zychaea mexicana
<400> 20
atgagaccag ttcatctttg gaaaagcctg cttttagctg cggctactgc agtcacgggt 60
acaatagctg ttgatgtacc ttcggtgcct attcagttgg agtcctatac ttattccgaa 120
aatgtattcg ctggtcgaat tttcgtaagt tatggaagcc ataataataa agtggcagga 180
tctatgatgc acaataacta aactctctga ttactcgtta actccctttg tattttgata 240
tctaggttca aaacattgcg tacaccaaag aagtcaacgt gttctggtcc gatgcttctg 300
atgactggaa cgacaatggc aactctgtgg cagctagtta ttccgaatcc atcgcagaca 360
ccaactatga gtactgggaa ttttcaacca cgattggatc tgccggtatc agccaaagtt 420
atttgagagt aagtcccaat cgccatttta tatatattgt gcacgtcagc aatagagctc 480
tgcagtaaag atataacttc agataacctt gacatctatg tctgaacaat ttcgggtttt 540
agtatgacgt atctggatca atctattatg acaataacga cagccagaac tacggtaagc 600
agagtatgag ctacatcttt gaagccttca taatgaagca gctgcacata tactgcctcc 660
taattgggtt gcacgtacaa cgggaaggat tattgctaac tttatatgtt acgtttgtac 720
cagatatcac cgagacatcg acccctacca caaccactgc tgctcccact tctaccacta 780
gcactcctac tactactacg gatggtggat cgacaactac taccactgcg acatctgttc 840
ctacgtctac tggtgttcca gagggcaatg ccaccatcag cgaatgggca agcgctcaat 900
tggatatcag ctggccaaac cttatgatga atgtaagtca acaatactct gtatagtgca 960
gcatacatta caagccattc aattaatcga attttcttac attctctttt tttgttccca 1020
ggtaaaccca tcaggtgcag tgactggctc tatcgttgcc tctttgtcga ctagtaaccc 1080
agattatttt tacatttgga cgcgagatgc tgcaatggtt gcacgcgtga tggtatacat 1140
gtacaacaca accgaagctg gtgacacaaa tctgttaaat gccttgaccg actatgttac 1200
gttctccatc agctccatga atgtggatac cgtctgcgac tgtttgggcg agcccaaatt 1260
caatgtggta agtagagaaa ttttataaaa acagggctaa gtgatactga agagcaagga 1320
taaaaaagtg aggacaaaaa gagtagcaat actagtgtta ataatatacg attcattata 1380
ggatggaagc ggttacaccg gtgcgtgggg aaggtacagt aattatggaa ctagaggaca 1440
gaaaaggctg tattttttgt attatgtgat atgcactgaa aggttggatg aatcttttgt 1500
ttgcaacaat gtactcacat tcatttatgc attagacccc aaaatgatgg acctgcagag 1560
cgtgcttcaa ccatgatttt gatcgcagac agctatatcg ctcagggcgg tgatgtttcc 1620
tatgttactg acacgctgaa gccaggtatg taaattttct atactttact caccatcagc 1680
aacacggagt tttcctgtca tagataacaa acgatcattg acacattatg gtctgtgatc 1740
ttttttatag ccatttacac cgatcttgat tatgttgttg atacttggtc taacgtttgc 1800
ttcgatcttt gggaagaagt gaatggcatt catatgtgta agctatttgt gataatcatt 1860
tttatatgat gtaaaaggaa agtctcttgg ctcactagta tgatgaaaca tagacacctt 1920
gtcggtgatg cgcaaggctc ttctcgatgg cgccaatttt gctacccgca atggcgacac 1980
ctcgcgtgtc agcggttatg aaagcaccgc ttcatccatc aagaccagac tcgaatcatt 2040
ctggtcctct agcaacaact acatcaccgt tacccaatcg ttctcgggag gtgtttcaaa 2100
ggccggcttg gatgtctcta cattgattgc tgccaacctt gcaagcatga acgatggtaa 2160
gtgattactt cacatgaaaa aggtgtatat ttttactcac atatgtctgt cccttgatgg 2220
ttgtaattta ggtttctata ctcctggctc tgatgaaatc ttggccacgg ctgtcgctat 2280
tgaaaactct tttataagtg agtatacgct caatcaaaac agaccatcgt ggctcagcac 2340
agctattggc cgatatcctg aagattctta tgatggctat ggtaactcgc aaggcaatcc 2400
agtaagtaaa aagagtagtt cctttacgta acgctactac tcgtagtgat gatgtgcaca 2460
agagcagcaa atccaattca gctgtagttc gattactgac aatgcaatta tgtcctctag 2520
tggttcattg ccactgccac atatgctgag ctctactacc gtgccatttt ggagtggcaa 2580
caacaagcat ccatcagtgt caactctgtc aacctgggct tctttagcaa gtttgattcc 2640
gatgcttctg tcggcactgt gtacacaccg ggcactgaag attttgccaa catggtctcc 2700
aacgtagcct tcgctgctga cgaattcttg gctaccattg agaaccattc tgccgttaat 2760
ggatccctct ctgagcaata taacagagat actggtatta tgcaaggcgc cagagatctc 2820
acctggtccc atgccgcatt tattactgct gcgaaggcaa agcagggtgc accaatacac 2880
tga 2883
<210> 21
<211> 1407
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 21
atgccttctt ggaaaaccct gttcctcctc ctgggcccta tcgccaccgc tgccgctgct 60
cccgttgaca agcagtctct gcctaccggc aacagcacca ttagctcttg ggtcagcaag 120
caggaggaca tcagcttcag cgagatgctc cgaaacgtca accctgaggg caccgccaag 180
ggcttcgttg ccgcgtcgct cagcaccgct ggccccgact acttctacac ctggacccga 240
gacgccgctc tcgtttcccg agttattgct tacaagtaca acaccaccaa cgctggcgac 300
tctaagatcc acggcgttct ggacgactac gttaacttcc agattaacac ccagtctgag 360
tctacccctt gcaactgcct gggcgagcct aagttcaacc ctgacggcag ctctttcacc 420
ggcccttggg gccgccccca gaacgacggc cccgctgagc gagcctccag tttcatgctg 480
attgccgact ctttcctcag ccagaccaag aacgcctctt acttcaccaa caccctcaag 540
cctgccattt acaaggacct cgactacgtc gttgacacct ggtctaaccc ttgcttcgac 600
ctgtgggagg aggttaacgg catccacttc tacaccctca tggttatgcg acgatctctc 660
ctcgacggcg ctaacttcgc cacccgaaac ggcgacaact ccaaggcctc aacctactcc 720
ggcgtcgccg ccaagattca ggctcgactg aactctttct gggacgccgg caagaactac 780
attaccgtta cccaggacta caagaacggc gttgagaagc ctagcggcct cgacgttagc 840
accctcattg ctgctaacgt cgctggcatg ggcgacggct tctacacccc cggcagcgag 900
cgcatcctcg ctaccgctct ggctttcgag aagagcatgg caagtctgta ccctctgaac 960
aacaacctgc ctagccacct gggcaacgcc attggccgat accccgagga cacctacaac 1020
ggcaacggca actcccaggg caacccttgg ttcctcgcta ccaccgcttt caccgagctg 1080
tactaccgcg ccattctgga gtggaagaac accggcgtta ccgtcacccc tatttctaag 1140
gacttcttcg tccgattcga cagcagcgct gctcctggca agaagtacaa ccccggctcc 1200
caggagttcg ctaccctgac ccagtctatt gctgccgccg ctgaccgatt catgtccacc 1260
gtccagtacc accagaaccc taacggctct ctgtctgagg agttcgaccg atctagcggc 1320
tacatgaccg gcgcccgaga cctgacctgg agtcacgctg ctttcattac cgctgctcag 1380
gcccgagctg gctctccttc tttctaa 1407
<210> 22
<211> 1818
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 22
atgaagctgt tcggcaccct gaaggcttct ttcgttttcc tgattggctg cattgttttc 60
gctatggccg acaccgtccc taccacccag gttaagctgc agtcttacac ctactctggc 120
ggcgtcctgt ctggcaccat ctacgtccag aacattgact acaccaaggt tgttaccgtt 180
atatattccg acggctctgg caactggaac aacaacggca acaccattag cgcttcttac 240
agcagctcca tttctggcac caactacgag tactggacct tctctgccag cgtcagctct 300
attcagcagt tctacatctc ttacctcgtt gacggcacca cctactacga caacaacggc 360
ggctacggcg ctaactacgc cgttaccgcc accaccacca ccgcttcttc caccaccaag 420
accacctccg gcaccaccaa gacctccacc accagcaccg ctaccgccac cagcaccggc 480
acctcttcct tccctagcgg caactctacc attagcacct ggagcccttc ccagcacagc 540
attagcctgt acgccatgct gcgaaacatt aaccctcccg gctctgctgc tggcttcatc 600
tctgctagtc tgtctacctc cggccccgac tactactact cttggacccg agacagcgcc 660
ctcgttgctc acgtcatcgt taacgagtac aacaccacct accagggcaa ctccaccctg 720
ctgggcatcc tcaaggacta cgtcacctac agcctgaacg cccagaccac ctctaccgtt 780
tgcaactgcc tgggcgagcc taagttcaac cccgacggct cctccttcac cggcgcttgg 840
ggccgccctc agaacgacgg ccccgctgag cgagctgttt ctttcatata tttcgctgac 900
tcttacctga cccagacctc tgacagctct tacgtcaccg gcaccctcgc ccccgctata 960
tacaaggacc tcgactacgt tgtttccgtt tggtctaacg gctgcttcga cctgtgggag 1020
gaagttaacg gcattcactt ctacaccctc atgttcatgc gacgaggcct gctcgacggc 1080
gctaacttcg caagccgaaa cggcgactct acccgagcga gcacctacac ctctaccgct 1140
gctagtatca agaccaagat tgacggcttc tgggtcagca gcggcaacta cgtccaggtt 1200
tcccagtctg ttacctctgg cgtctctaag gctggctacg acgcttccac cctgattgcc 1260
gctaaccagg cctctcgggg cgacggcttc tacacccccg gctccgacaa gatgctcgct 1320
accgccgttg ctattgagtc tcagttcagc agcctgtact ctattaacac caacaaggct 1380
tcttacctgg gcaacgccat tggccgatac cccgaggaca cctacaacgg caacggcaac 1440
tcccagggca acccttggtt cctgtgcacc aacgctttcg gcgagctgtt ctaccgcgcc 1500
attagcgagt ggaacgccgc tggctccgtt accgttaaca gcgttaacct cgctttcttc 1560
aagaagtacg actctagcac cagcagcggc accacctaca ccgttggcac cagcgcttac 1620
aacaacctgg ttcagaacgt tgccctcgct gccgacgctt acttctctac cgtcaagtac 1680
cacgccctga ccaacggctc catgtctgag cagtacgacc gatctagcgg catggctacc 1740
ggcgcaagag acctgacctg gtctcacgcc gccatgatta ccgccctgaa ggccaagtcc 1800
ggcacccccg tttactaa 1818
<210> 23
<211> 1809
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 23
atgaagcctt tcggccctat caagaccacc ctgttcatta ttatctctca cttcagcctg 60
aactccctcg ctgacaccgt tcctaccacc caggttgagg tcaagaccta cacctactct 120
ggcggcgagc tgtctggcac catatacgta gagaacatag actacaccaa ggttgttacc 180
gtcatctacg ctgacggctc tgacgactgg aacaacaacg gcaacaccat tgctgccgct 240
ttcagcgagt ccatctctga caccaactac gagtactgga ccttctctag ctctgttaac 300
tctattaaag agttctacgt caagtacgac gttgacggca acacctacta cgacaacaac 360
ggcaacgcta actaccaggt ttctgctagt agcaccacca ccaccaccgc caccaagacc 420
accaccaccg ctaccaagac caccgccacc gctaccagca ccgccaccgg cagcacctct 480
ttccctagcg gcaacagcac catcagctct tggattaagg gccaggaaga cacctcccgg 540
tccgtcatgc tcggcaacat taaccctcct ggctctgcta ccggcttcat tagcgccagc 600
ctgtctacct ctggccccga ctactactac cactggaccc gagacgctgc cctcgttgct 660
cacgttattg ttaacgacta caacaccacc ctgtctggcg actctagcac cctgcaggtc 720
atcaaggact acgttacctt cagcgtcaac tcccagtccg agtctaccgt ttgcaactgc 780
ctgggcgagc ctaagttcaa ccccgacggc tcctcttaca ccggcgcttg gggccgcccc 840
cagaacgacg gccccgctga gcgagctacc accttcattt tcttcgccga cacctacctc 900
gcccagggcg gcgacagctc ttacgttacc ggcaccctcg cccccgctat atatgctgac 960
ctggactacg ttgttaacaa ctggtctacc ggctgctacg acctgtggga agaggttaac 1020
ggcattcact tctacaccct catggttatg cgccgaggcc tcatagatgg cgcttccttc 1080
gcttcccgaa acggcgacag cacccgaagc tcctcttaca cctccaccgc caagtctatt 1140
gctaccaaga ttgactcttt ctggtctgct tctaacaact acgttgctgt ttcccagagc 1200
gttacctctg gcgtctccaa ggctggctac gacgcctcta ctattattgc tgctaaccag 1260
gcgtcgctgg gcgacggctt ctacaccccc ggctccgaca agatgctcgc tacctccgtt 1320
gccgttgaga acgctttcag cagcctgtac accgttaaca ccggcaaggc ttcttacctg 1380
ggcaacgcca ttggccgata ccctgaggac acctacaacg gcaacggcaa ctctcagggc 1440
aacccttggt atctgtgcac caacgccctg ggcgagctgt actaccgcgc catcaaagag 1500
tggaacgctg cgggaagcgt taccgtcaac tccgttaacc tgggcttctt ccagaagatt 1560
gactccagca ttagcagcgg caccaccttc accctgggca cctccgacta cagcaccctc 1620
gttgacaacg tcgccctcgc cgctgacaag ttcttcgccg tcgtccagta ccacgagcga 1680
tctaacggct ccatgcctga gcagttcggc cgagaggacg gcctgcctac cggcgcgaga 1740
gacctgacct ggtctcacgc cgccatgatt agcgctgccc gagctaaggc tggcaccccc 1800
gtttactaa 1809
<210> 24
<211> 1806
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 24
atgaagttct ctcacctgtt ccgaaagcct accctcctca ttgttacctt cctgaccgcc 60
accgttttcg ccgagaccgt ccctaccacc tcccaggtta agctcaagtc ttacacctac 120
gacggctcta ccctgtctgg ccagatttac atccagaaca ttgcttacac caaggttgtt 180
accgtcattt actctgacgc cagctctaac tggaacaaca acggcaacac cattgctgcc 240
tcttacacca acggcattag cggcaccaac tacgagtact ggaacttcac cggccccgtc 300
agctctatta acgccttcta cattaagtac gacgtttctg gcaacaccta ctacgacaac 360
aacaacaacg ctaactacca ggttaccaag acctccacca ccaccaccac cagcaccgcc 420
accgctacca ccaccaccaa gacctctacc cctacctcca ccggcgttcc ttctaacttc 480
cctaccggca actctaccat cagcacctgg ctcaagtccc agattggcat ctctcgatac 540
gccatgctgc gaaacattaa ccccgctggc tctgccgttg gcttcattgc tgctagcctc 600
agcaccgcta accccgacta ctactacgct tggacccgag acagcgccct gacctcttac 660
gtcatcgcta acgactacaa ctctaccctc gctggcaaca gcaccatcct gcagattatg 720
aaggactacg ttaccttcag cgtcaagtct cagtccgttt ctaccgcttg caactgcctg 780
ggcgagccta agttcaaccc cgacggcagc tcttacaccg gcgcttgggg ccgcccccag 840
aacgacggcc ccgccgagcg agctaccacc ttcatcctgt tcgctgactc ttacctgaag 900
cagaccggcg acgcttctta cgttaccggc accctgaagc ccgctatttt caaggacctc 960
gactacgttg ttaacacctg gaccaacggc tgcttcgacc tgtgggagga ggttaacggc 1020
gtccacttct acaccctcat ggtcatgcga aagggcctga tccgaggcgc caacttcgct 1080
acccgaaacg gcgacagcac ccgagctagc acctacacca acaccgctgc tagcatcaag 1140
accaagatgg actctttctg gagctctggc aacaactaca ttgctgtctc ccagtctgtt 1200
accggcggcg tctccaaggc tggctacgac gttgctaaca ttattgccgc taacgttggc 1260
agcctgcagg acggcgttta cacccccggc tccgaccgca ttctcgccac cgccgttgcc 1320
gttgaggcta agttcgccag cctgtacggc gttaactcta acctccccgg ctacctgggc 1380
aactctattg gccgataccc tgaggacacc tacaacggca acggcaactc ccagggcaac 1440
ccttggttcc tcgccaccaa cgcttacgcc gagctgtact accgcgccat tcgcgagtgg 1500
tacgacaacg gcggcgtcac cgttaactct gttaacctcc ctttcttcaa gaagttcgac 1560
agcagcgctg ctagcggcac cacctacacc gttggcacca ccgctttcaa caccatggtt 1620
tctaacgttg ctgctgccgc tgacaagttc ttcagcaccg tcaagttcca cgcttacacc 1680
aacggctcca tgtctgagca gttcggccga aacgacggcc tgtgcaccgg cgcaagagac 1740
ctgacctggt ctcacgctag cctgattagc gctgccctcg ccaaggctgg cacccctagc 1800
gtctaa 1806
<210> 25
<211> 1797
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 25
atggtttctt tcaacttcct gaagaagcct gttttcattg ttatcacctg cctcaccgtc 60
agcgtttgcg cccagtctgt tcctaccagc gaccccgtta aggttaagac cttctcttac 120
gacggcaact ctttctctgg ccagatttac atcaagaaca ttgcttacga gaagaccgtt 180
accgttattt actccgacgg caccggcaac tggaacaaca acaacaacaa gattgccgct 240
gctttcagcg aggccatctc cggctctaac tacgagtact ggaccttcag cgctagcgtt 300
cctagcatca agcagttcta cgtcaagtac gacgtttccg gcaagaccta ctacgacaac 360
aacggctcta aggactacaa cgtcgttacc agcggcccta ccaccaccag ctctggccct 420
agcaagacca ccaccaacgg ccccgctcct accagcacca acaccaactt ccctagcggc 480
aaccctagca ttacctcttg gattgacaag cagattgaca tctcccgatc tgccatgctg 540
aagaacatta accccgctgg caccgttaag ggcttcattg ccgccagcct cagcacctct 600
aaccccgact acttctacgc ttggacccga gacgccgctc tcgttgccca cgttgttgct 660
aacgactaca accgaaccaa gtctggcgac gccacctacc tgggcctcct gaaggactac 720
gtcaccttct ctattaactc ccagaacacc cctaccgctt gcaactgcct gggcgagcct 780
aagttcaaca aggacggctc cggctacaac ggcccttggg gccgccctca gaacgacggc 840
cccgccgagc gagctgacac cttcgtcctc attgccgact ccattctgac ccagaccaag 900
gacgtctctt acgttaccgg caccctgcgc cccgctattt acaccgacct cgactacgtt 960
gtccgaacct ggtctaacgg ctgcttcgac ctgtgggagg aggttaacgg cgtccacttc 1020
tacaccctca tggttatgcg acgagctctc ctcgttggcg ctaacttcgc tagccgaaac 1080
ggcgactctg cccgagcttc taactacaac aacgctgcta actccattaa gtctaagatt 1140
gactctttct ggtcctctaa caacaactac gttgctgttt cccagtctgt taccggcggc 1200
gtttccaagg ctggctacga cgtcagcacc ctcatcgctg ctaacgttgg cagcctgtct 1260
gacggcttct acacccctgg ctctgagcgc atgctcgcca ccgccgtcgc tattgaggac 1320
aagttcgcta acctgtacgg cattaaccga aacctcgaca gcagcctggg caacgccatt 1380
ggccgatacc ccgaggacac ctacaacggc aacggcaact cccagggcaa cccttggttc 1440
atcgccacca acgcttacgc tgagctgtac taccgagcta ttaaggagtg gaacaacaac 1500
ggcggcgtca ccgtcaccaa cgttaacctg aacttcttca agaagttcga cggcagcgct 1560
agcgttggca ccaagtacac cgctggctct gctgcttaca acaccctgac ccagaacatc 1620
gccctcgccg ctgacaagtt cttcaacacc gtcaaggtcc acgctgccac caacggctcc 1680
atgtctgagc agtaccaccg agacaccggc agcatgaccg gcgcaaggga cctgacctgg 1740
tctcacgcta gcctgattac cgccgccctc gccaaggctg gcacccccgt cgcctaa 1797
<210> 26
<211> 1830
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 26
atgaagctgt tcggcctgcc cctcaagcct agcctgttct tcgtcgtctc ttacctgtct 60
ttcctcgctt ctgctgccag catccctagc agcagcgagg tccagctcga cacctacgct 120
tacgacggcg ccaagttctc tggcaagatt tacgtcaaga acattgctta cgagaagacc 180
gttaccgttg tttacgctga cggctctgac aactggaaca acaacggcaa cattattagc 240
gctaccttca gcacccctat ttccggctct aactacgagt actggacctt cagctctagc 300
attagcggca ttaaggagtt ctacatcaag tacgtcgttt ccggcaagac ctactacgac 360
aacaacggca ccaagaacta ccaggtttcc accaccagct ctaccaccac cgctagcacc 420
accaccgcta cccgaaccac caccgctggc accaccagca cctctaccac cgctgcccct 480
acctctacct ccagcggctc cttccctagc ggcaactcta ccgttagctc ttggattaag 540
cgacaggaga agatctcccg attcgccatg ctgcgaaaca ttaacccccc tggcagcgct 600
gctggcttca ttgctgctag cctgtctacc tctggccccg actactacta ctcttggacc 660
cgcgacagcg ccctgacctc taacctgatt gcttacgagt acaacaccac cctgtctggc 720
aacaccacca ttctgaacat cctgaaggac tacgttaagt tctccatcag ctcccagacc 780
accagcaccg tttgcaactg cctgggcgag cctaagttca accacgacgg cagctcttac 840
accggcgctt ggggccgccc ccagaacgac ggccccgctg agcgagctaa caccttcatc 900
ctgttcgccg actcttacct cgaccagacc aaggacgctt cttacgtcac cggcaccctg 960
aagcccgcta ttttcaagga cctcgactac gttgttaacg tttggtctaa cggctgctac 1020
gacctgtggg aggaggttaa cggcgttcac ttctacaccc tcatggtcat gcgaaagggc 1080
ctcctgctgg gcgctgactt cgctaagcga aacggcgact ctacccgagc tagcacctac 1140
accaacaccg ctagcaccat tgctaccaag atctcctctt tctgggttag cagctctaac 1200
tggatccagg tttctcagtc tgttaccgcc ggcgtttcca agaagggcct cgacgtcagc 1260
accctcctcg ctgccaacct gggctccgtt gaggacggct tcttcacccc cggctccgag 1320
aagattctgg ccaccgccgt tgccattgag gacgctttcg ctagcctgta ccctattaac 1380
tctaacctgc cttcttacct gggcaactcc attggccgat accccgagga cacctacaac 1440
ggcaacggca actcccaggg caacccttgg ttcctcgccg ttaacggctt cgccgagctg 1500
tactaccgag ctatcaagga gtgggttaac aacggctccg ttaccgtctc taacatctcc 1560
ctgtccttct tcaagaagtt cgacagcagc gccaccgccg gcaagaccta caccgccggc 1620
accgctgact tcaacaacct cgcccagaac attgctctgg gcgctgaccg attcctgtcc 1680
accgtccaga cccacgcttt caacaacggc tccctcgccg aggagtacga ccgaaccacc 1740
ggcgtcagca ccggcgcccg agacctgacc tggtctcacg ctagcctcat taccgctgct 1800
tacgccaagg ctggcagccc tgccgcctaa 1830
<210> 27
<211> 1827
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 27
atgaagttct ctaacctcct gaagaagccc ctcctgctca ttgctggcat tctcgctgcc 60
accgtcgttg ccgagaccgt ccctaccacc gctgctgtta aggttaagtc tttcacctac 120
gacggcagca ccttcgctgg ccagatttac gtcaagaaca tcgcttacac caagaccgtt 180
accgttattt actctgacgc ctctaacaac tggaacaaca acggcaacac cattgccgcc 240
tcttacagcg agggcatctc tggcaccaac tacgagtact ggaccttcag cgcccctgtt 300
tccggcatca agcagttcta cgtcaagtac gttgtcagcg gcaccaccta ctacgacaac 360
aacaactctg gcaactacca ggttaccacc accaccacca ccaccgcccc taccagcacc 420
acctccggcg gctctagcac caccaccggc ggctccacca ccaccgccac ctctgtccct 480
accggcgtcc ctagcggctt ccctaccggc aacagctcta ttagctcttg gattgacggc 540
cagacctccg tttctcgata cgccatgctg cgaaacatta accccgctgg cgctgtttcc 600
ggcttcattg ctgctagcat gtctacctct ggccccgact acttctacgc ttggacccga 660
gacagcgccc tgacctctca cgtcgttgct tacgactaca acaccaccct cgctggcaac 720
tctaccatcc tgggcctcct gaagaactac gttaccttct ctattaactc tcagaccacc 780
agcaccgttt gcaactgcct gggcgagcct aagttcaaca aggacggcag ctcctacacc 840
ggcgcttggg gccgacccca gaacgacggc cctgctagcc gagctgacac cttcatcctc 900
attgccgact ctattctgaa gcagaccggc gacgctacct acgttaccgg caccctcgcc 960
cccgctattt acaaggacct cgactacgtt gtcagcacct ggtctaacgg ctgcttcgac 1020
ctgtgggagg aggttaacgg cgttcacttc tacaccctca tggtcatgcg acgaggcctg 1080
attaagggcg ctaacttcgc ttcccgaaac ggcgacaaca cccgagctaa cacctacacc 1140
aacaccgccg ctagcatcaa gaccaagatt gactctttct ggaactctaa cggcaactac 1200
gttaccgttt cccagtctgt taccggcggc gtctccaagg ctggctacga cgctagcgtc 1260
ctgattgctg ctaacctggg ctctgtccag gacggcttct acacccctgg ctccgacaag 1320
atgctcgcta ccgctgttgc cattgagtct aagttcgcca gcctgtactc cattaaccag 1380
aacctgaacg gctacctggg caacgccatt ggccgatacc ccgaggacac ctacaacggc 1440
aacggcaact cccagggcaa cccttggttc atttgcacca acgctttcgc cgagctgtac 1500
taccgcgcca ttaaggagtg gttcaacaac ggcggcgtta ccgttacctc catcagcctg 1560
aacttcttca agaagttcga cagctctgct gccgttggca ccaagtacac cgttggcacc 1620
tccgccttca acagcctcgt ccagaacgtc gccgtcgctg ccgacgcttt cttcagcacc 1680
gtcaagttcc acgccgctac caacggctcc atgagcgagc agtacggccg atctgacggc 1740
ctcatgaccg gcgccagaga cctgacctgg tctcacgcta gcctgattag cgcttcttac 1800
gccaaggctg gcagccccgc cgcctaa 1827
<210> 28
<211> 1809
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 28
atgaagctga actccgtttg gaaggctttc agcattctgc tcatggctac tatggctttc 60
gctgctaccg tccccaagac ccaggttaag ttagaggctt acacctactc tgactctatt 120
ttctctggcc gaattttcgt ccagaacatc gactacacca agattgttac cgtttactgg 180
tctgacgcct ctaacaagtg ggacagctct aagtactaca ccgaggccgc ttacacccac 240
agcatccccg gcaccaacta cgagtactgg gacttcagcg ctaccattgg ccctagcggc 300
atcaagcagt tctacatctc ttaccaggtc cgaggcgtca cctactacga caacaacggc 360
ggctacggcg tcaactacga cgtcatctcc agccctccta ccacctctga cgccaccacc 420
attcctacca ccaccacctc cgctattcct agctctacca ccaccatccc tgccagcacc 480
ggcgtcccta gcggcaactc caccattacc gtttgggctg actcccagca caagatttct 540
tggaaggcta tgctcgctaa cattaaccct cccggcagcg ccaccggctt cattgctgcc 600
tccctgtcta cctccggccc tgactactac tacgcttgga cccgagacgc cgcaatggtt 660
gctcacgtta tcgttaacgc ttacaacacc accaaggctg gcgacgctac caccctgggc 720
gttctgaagg acttcgttac cttccagatc aaggctatgg ctacctctac cgtttgcaac 780
tgcctgggcg agcctaagtt caaccccgac ggctcctctt acaccggccc ttggggccgc 840
ccccagaacg acggccctgc tgagcgcgct accaccttca tcctgttcgc cgactcttac 900
ctgtctcaga ccggcgacgc tgcttacgtc accaccctga agcgagccat tttcaccgac 960
ctcgactacg ttgttaccac ctggcaggac aactgcttcg acctgtggga ggaggttaac 1020
ggcctgcaca tgtacaccct cgctgttatg cgacgatctc tcgttgacgg cgcctctttc 1080
gccgctcgaa acggcgacaa cacccgagcc tctacctaca ccaacaccgc caagtctatt 1140
gagaccaagc tcgcctcttt ctacaacagc tcctctaact acgtcgttgt taccaagaac 1200
ttcgctggcg gcgttaacaa ggctggcctg gacacctcca ccctgattgc tgccaacacc 1260
gctggcctgg gcgacggctt cttcaccccc ggcagccccg agatcctcgc taccgccgct 1320
gccattgaga agtctttcgc cgacctgtac ggcattaaca agcagattcc taactggctg 1380
ggcaccgcta ttggccgata ccccgaggac acctacaacg gcaacggcaa ctctcagggc 1440
aacccttggt tcatttgcac cgccaccttc gccgagctgt actaccgagc tattctggag 1500
tggcagcacg ccggcagcgt taccgtcaac aacgttaacc tgcctttctt caagaagttc 1560
gactctagca cctccagcgg caccacctac accgttggca ccagctcctt cgacagcctg 1620
attagcaagg ttgcttacgc cgctgacaac ttcttctcta ccattaagta ccacgctgcc 1680
accaacggca gcatgtctga gcagttcaac cgagacaccg gcttcatgac cggcgccagg 1740
gacctgacct ggtcgcacgc tgccttcatt accgccgcta aggctaaggc tggcaccccc 1800
gtttactaa 1809
<210> 29
<211> 1821
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 29
atgcgcccta gcctcgtttg gaagtctctc ctgatggttg tcctgaccgc tgcaatgggc 60
acctacgccc agaccgtccc tagcgttcct attaagttag aatcttacac ctactctgac 120
aacgttttcg ctggccgaat tttcatccga aacattgctt acaccaaggt tgtcaaggtt 180
ttctggtctg acgagtccgg caactggaac ggcaacggca actacgttga cgcctcttac 240
tctggctcca tctccggcac caactacgag tactgggagt tcagcaccac cattggctct 300
gccggcatct cccagtctta cctgcgatac gacgtcagcg gctctaccta ctacgacaac 360
aacggcaacg ctaactacga cattgaggag gacagcaccc ctacccctac caccaccacc 420
acctctacca ccaccatcac caccaccacc acctccggca cctctacccc tacctctgtt 480
cctaccgagg tccctgccgg caacaccacc gtcaccgact gggttaacga ccagttagaa 540
atttcttggc ctagcctcct caagaacgtc aaccctagcg gcgctgttac cggcttcatt 600
gctgcatctc tgtctaccaa cgaccccgac tacttctact gctggacccg agacgccgcc 660
ctcgttgccc gcgtcatggt ttacatgtac aacaccaccg aggctggcga cacctccctg 720
cgatctaagt tacaggacta cgttaccttc cagattaact ccatgaaaac cgccaccgtt 780
tgcaactgcc tgggcgagcc taagttcaac aaggacggct ccggctacag cggcgcttgg 840
ggccgccctc agaacgacgg ccccgctgac cgagctatta ccctgattct gttcgctgac 900
tctttcatcg cccagggcgg cgatgtttct tacatcacca acaccctgaa gcctgctatt 960
tacaccaacc tggactacgt tgttaacacc tggtctaacg tttgcttcga cctgtgggag 1020
gaggttaacg gcgtccacat ttacaccctg tctgttatgc gaaagggcct cctggaaggc 1080
gctgacttcg cttctcgaaa cggcgacagc acccgcgcca acacctaccg atctaccgcg 1140
tcctccatca agacccgact ggagtccttc tggagcagct ctaacaacta cattaccgtc 1200
acccagtctt actctggcgg cgttaacaag gctggcctgg acgtcagcac cctcctcgcc 1260
gctaacggcg ccagcatgaa cgacggcttc tacacccccg gctctgacaa gatgctcgct 1320
accgccgttg ccattgagaa ctccttcgcc ggcatttacg ccgttaacca gaaccgcccc 1380
gactggaagg gcaccgctat tggccgatac cctgaggaca cctacgacgg ccacggcaac 1440
tcccagggca acccttggtt cattgctacc gccgcttacg ctgagatgta ctaccgagct 1500
atactggaat ggcagcagca gccttccatt accgttaact ctattaacct cagcttcttc 1560
aagaagttcg actctagcgc tgctgttggc accgtttaca agcccggcac ccaggctttc 1620
aacaacatgg tttctaacgt tgctttcgct gccgacgagt tcttcagcac catgaacttc 1680
cacagcgcta ccaacggctc tatgagcgag cagtacaacc gaaacaccgg cattatgcag 1740
ggcgcaagag acctgacctg gagccacgct gctttcatta ccgctgctaa ggccaagctg 1800
ggcacccccg ttttctaata a 1821
<210> 30
<211> 1809
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 30
atggttttca gcaagctgtt caagaagcct attctcctga ttgttaccta cctgaccgtt 60
accgtcgtcg ccgagaccgt tcctacctct gctcaggtcc aggttaagtc tttcaactac 120
gacggcagca ccctgtccgg ccagatttac gtccagaaca ttgcttacga aaagaccgtt 180
accgttgttt actctgacgg ctccgacaac tggaacaaca acggcaacac cattgctgcc 240
tcttacagct ctagcattag cggctctaac tacgagtact ggaccttcag ctctagcgtc 300
cccagcatta agcagttcta catcaagtac gttgtcgctg gcaagaccta ctacgacaac 360
aacggcacca agaactacca ggtttccgct tccaccccta ccaccaccac caccaccacc 420
tcctctggcg ccaccaagac caccaccacc atcggcccta ccagcaccag caccgttttc 480
cctagcggca acagcaccat cagctcttgg ctgaagggcc agattgagac ctcccgattc 540
gccatgctgc gaaacattaa ccccgctggc accgtcaagg gcttcatcgc cgcctcgctg 600
tctaccgcta accccgacta cttctacgct tggacccgag acgctgctct cgtcggccac 660
gtcattgcta acgactacaa ccgaaccctc gctggcaaca gcacctacct gggcctcctg 720
aaggactacg tcaccttctc tgttaactcc cagtctacct ccaccatttg caactgcctg 780
ggcgagccta agttcaacaa ggacggctct ggctactctg gcgcttgggg ccgccctcag 840
aacgacggcc ccgccgagcg agctgacacc ttcatcctca ttgccgactc catcctgaag 900
cagaccggcg acgccaccta cgttaccggc accctgcgcc ctgctattta caaggacctc 960
gactacgtcg ttaacgtttg gtctaacggc tgcttcgacc tgtgggagga ggttaacggc 1020
gtccacttct acaccctcat ggttatgcga cgatctctga tcctgggcgc taacttcgct 1080
tctcgaaacg gcgacagcac ccgagcgtcc acctacacca acaccgctaa ctctattaag 1140
accaagattg acaccttctg gtcttcctct aacaactacg ttgctgtttc ccagtctgtt 1200
accggcggcg ttaacaaggc tggctacgac gttgctaacc tcatcgccgc taacgttggc 1260
agcctcgacg acggcttcta cacccccggc agcgagcgaa tactcgccac cgctgttgct 1320
gttgagtcta agttcgcttc gatttacggc gtcaaccaga acctgccttc ttggctgggc 1380
aactccattg gccgataccc cgaggacacc tacaacggca acggcaactc tcagggcaac 1440
ccttggttca ttgctaccaa cacctacgcc gagctgtact accgcgctat taaggagtgg 1500
accaacaacg gcggcgttac cgtcaccaac gttaacttca acttcttcaa gaagttcgac 1560
agctccgctt ctgttggcac caagtacacc gttggcacca gcgctttcaa caccctgacc 1620
cagaacgttg ccctcgctgc cgacaacttc ttctctaccg tcaaggttca cgctgccacc 1680
aacggctcca tgtctgagca gttcggccga gactccggcg tcatgaccgg cgcccgcgac 1740
ctgacctgga gtcacgcatc actgattacc gccgccctcg ccaagaccgg cgcccctgtt 1800
gcctaataa 1809
<210> 31
<211> 1833
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 31
atgaagttct ctaacctcct gaagaagccc ctgctcctga ttgccggcat tctcgctgtt 60
accgtcgttg ccgagaccgt ccctaccacc gaggctgtta aggtcaagtc tttcacctac 120
gacggcagca ccctcgctgg ccagatttac atcaagaaca tcgcttacac caagaccgtt 180
accgttattt actccgacgc ctccgacaac tggaacaaca acggcaacac cattgctgct 240
tcttactccg ctgctattgc tggcaccaac tacgagtact ggaccttcag cgcccccgtg 300
tccggcatta agcagttcta cgtcaagtac gttgtttctg gcaccaccta ctacgacaac 360
aacaactctg gcaactacca ggtttccgtt accaccacca ccaccaccgc ccctaccacc 420
accacctctg gcggctctag caccaccacc ggcggcagca ccaccaccgc cacctccgtc 480
cctaccggcg tccctagcgg cttccctacc ggcaacagca ccattagctc ttggattgac 540
ggccagacct ctgtttcccg atacgccatg ctgcgaaaca ttaaccctgc tggcgctgtt 600
accggcttca ttgccgcatc catgtctacc tctggccccg actacttcta cgcttggacc 660
cgagacagcg ccctgacctc tcacgtcgtt gcttacgact acaacaccac cctcgccggc 720
aacagcacca tcctgggcct cctgaagaac tacgttacct tcagcctgaa ctctcagacc 780
acctctaccg tttgcaactg cctgggcgag cctaagttca acaaggacgg ctctggctac 840
tccggcgctt ggggccgccc ccagaacgac ggccccgcta gtcgagctga caccttcatc 900
ctcatcgctg acagcattct gaagcagacc ggcgacgcca cctacgtcac cggcaccctc 960
gcccccgcta tttacaagga cctcgactac gttgtctcta cctggtctaa cggctgcttc 1020
gacctgtggg aggaggttaa cggcgttcac ttctacaccc tcatggttat gcgacgaggc 1080
ctcgtcaagg gcgctaactt cgcatcccga aacggcgact ctacccgagc taccacctac 1140
accaacaccg ccgcgagcat taagaccaag attgactcct tctggaactc taacggccag 1200
tacgtcagcg tttcccagtc tgttaccggc ggcgtttcca aggctggcta cgacgccagc 1260
gtcctgattg cttctaacct gggctctctc caggacggct tctacacccc tggcagcgac 1320
aagatgctcg ccaccgctgt cgccattgag tccaagttcg ccagcctgta ctccattaac 1380
cagaacctga acggctacct gggcaacgct attggccgat accccgagga cacctacaac 1440
ggcaacggca actcccaggg caacccttgg ttcatttgca ccaacgcttt cgccgagctg 1500
tactaccgag ctattaagga gtggttcaac aacggcggcg ttaccgttac ctccattagc 1560
ctgaacttct tcaagaagtt cgacagctct gccgccgttg gcaccaagta caccgttggc 1620
accagctctt tcaacagcct cgtccagaac gttgctgttg ctgccgacgc tttcttcagc 1680
accatcaagt tccacgccgc taccaacggc tccatgagcg agcagtacgg ccgaaccgac 1740
ggcctcatga ccggcgctag agacctgacc tggagtcacg catctctgat cagcgcctct 1800
tacgctaagg ctggctctcc cgctgcctaa taa 1833
<210> 32
<211> 1830
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 32
atgatgccta accacatttg gaagtgcctg ttcctcgcca tgaccaccgt tttcatgacc 60
gtcctcgtcc agggcgctcc cacctctgag attgaactag actcttacac ctacaccggc 120
ggcgttttct ccggccgact gtacgtcaag aacatcgctt acaccaagga ggttaacgtt 180
tactggtctg acgccagcga ggactgggct aacaacggca actacgttgc cgctacctac 240
tctgaggcta tctccggcac caactacgag tactgggagt tcagcgctaa gattggctct 300
agcggcatca gcgagtctta catcaagtac accgtttccg gctctaccta ctacgacaac 360
aacggcggca agaactacgc catcaccgag accaccaccc ctaccattac cgccacccct 420
acctctacca ccgctaccac caccattagc tctaccacca ccaccgccac ctccatccct 480
acctctgttc ctagcaccgt ccccgagggc aacgttaccg tcaccgagtg ggttaacaag 540
cagctcaaaa tatcttggtc cgacctcttg caaaacgtta acccttctgg caccgttacc 600
ggcttcattg ctgcctcact cagcacctcc aaccccgact acttctactg ctggacccgc 660
gacgccgcaa tggttgcccg cgttatgacc tacatgtaca acaccaccga ggctggcgac 720
agcagcctgg agagcgccct gaaggactac attaccttcc agattaactc tatgaaaacc 780
gctaccgctt gcaactgcct gggcgagcct aagttcaaca ccgacggctc cggctactcc 840
ggcccttggg gccgccccca gaacgacggc cctgctgagc gcgctaccac catgattctg 900
ttcgctgact cttacctcgc ccagggcggc gacacctctt acgttaccaa caccctgaag 960
cctgctattt acaccaacct ggactacgtt gttggcacct ggtctaacaa ctgcttcgac 1020
ctgtgggagg aggttaacgg cgttcacatt ttcaccctcg ccgtcatgcg aaagtctctc 1080
ctcgacggcg ctgacttcgc tgcccgaaac ggcgacacct cccgcgttag cggctaccag 1140
agcaccgctt ctagcattaa gaccaagctg gagtctttct ggtctagctc caacaactac 1200
attaccgtca cccagtctta ctctggcggc gtccagaagg ccggcctgga cgtcagcacc 1260
ctgattgctg ctaaccaggg ctcgatgggc gacggcttct acacccctgg ctctgacaag 1320
atgctcgcca ccgctgttgc tattgagaac tctttcgcta acatttacac cattaacaag 1380
aacaagcctt cttggctggg caccgctatt ggccgatacc ccgaggacac ctacaacggc 1440
aacggcaacg gccagggcaa cccttggttc atcgctaccg ccacctacgc cgagctgtac 1500
taccgagcta ttttggaatg gcagcagcag gagtctgtta ccgttaacag cgtcaacttc 1560
gacttcttct ctaagttcga cagcagcgcc aaggttggca ccgtttacac ccctggcacc 1620
gacaccttca acactatggt ttctaacgtt gctttcgctg ccgacgagtt cctcagcact 1680
atggagcact acgctgccac caacggctct atgtctgagc agttcaaccg agagaccggc 1740
agcctgaccg gcgcccgaga cctgacctgg tcgcacgctg ctttcattac cgctggcaag 1800
gccaagctgg gcattcctag cttctaataa 1830
<210> 33
<211> 1830
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 33
atgaagttca gcaagctgtt caccaagcct accctgttca tcgtttctat tattatcagc 60
gttgcttcta gcgagaccgt ccctaccacc gctaacgtcc tcgttaagtc ttacacctgg 120
gacggcgcta ccctgtctgg ccagatttac attaagaacc tcgcttacgc caaggttgtt 180
agcgttattt actctgacgc taacgacaac tggaacaaca acggcaacaa ggttgccgct 240
tcttactctg ctggcattga cggcaccaac tacgagtact ggaccttcag cggcgccgtt 300
tccggcatca agcagttcta cgtcaagtac gacgtcagcg gcacctctta ctacgacaac 360
aacggcacca agaactacca ggttaccaag acctctacca ccaccaccgc cgctaccacc 420
accaccaccg ctaccaccac caccggcggc accggcacca ccaccaccac caccgccacc 480
gccaccgcta ccagcaccga cttccctacc ggcaacagca ccattaccac ctggctgaag 540
tcccaggagg acatctcccg aggcgccatg ctccgaaaca ttaacccccc tggcgctgct 600
accggcttca ttgctgccag cctgtctacc tccggccccg actactacta cgcttggacc 660
cgagactccg ccctgacctc tcacgtcatc gctcacgact acaacaccac cctcgctggc 720
aacaccacca tcctgaacat cctgaaggac tacgttacct tctccgtcaa gtcccagtcc 780
gtttctaccg tttgcaactg cctgggcgag cctaagttca accccgacgg cagctcttac 840
accggcgctt ggggccgccc ccagaacgac ggccctgccg agcgagcctc caccttcatc 900
ctgttcggcg actcttacct gaagcagacc ggcgacgcta cctacgttac cggcaccctc 960
gctcctgcca tttacaagga cctcgactac gttgttaaca cctggtctaa cggctgcttc 1020
gacctgtggg aggaggttaa cggcgttcac ttctacaccc tcatgtctat gcgacgaggc 1080
ctcctcgacg gcgccaactt cgccaagcga aacggcgaca ccacccgagc taccacctac 1140
accaacaccg ccgcttccat tgctaccaag attgacacct tctgggttag ctctggcaac 1200
tacgtccagg tttcccagtc tgttaccggc ggcgtcagca aggctggcta cgactgcgct 1260
aacattctcg ctgctaacat ggctgctaac cacgacggct tctacacccc tggcagctct 1320
aagattctcg ctaccgccgt tgccattgag tctaagttcg ccagcctgta ctctattaac 1380
tctggcctcg cttcttggct gggcaccgcc attggccgat accccgagga cacctacaac 1440
ggcaacggca actctcaggg caacccttgg ttcatttgca ccaacgcttt cgctgagctg 1500
tactaccgag ccatcaagga gtggacccag gctggctccg ttaccgttga cagcacctct 1560
ctgaacttct tcaagaagtt cgacagctct gctgccgccg gcaccaagta caccgttggc 1620
acctctgctt tcaccaacct cgtccagaac attgctaacg gcgctgacaa gttcctgtct 1680
acctccaagt tccacgccgc taccaacggc tccatgtctg agcagtacaa ccgagacagc 1740
ggcctcatga ccggcgcccg agacctgacc tggtcgcacg cctcactgat tagcgcctcc 1800
cgagctaagg ctggctctcc tagcctctaa 1830
<210> 34
<211> 1845
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 34
atgaagccta ttcacctgtg gcactacctg ttcctcgcca tgaccgctgc cttcatgggc 60
gtcctcgctg acgtccctac ctccgccaac attgaactcg actactacac ctacaaggac 120
aaggttttct ccggccgaat ttacgtcaag aacatcgctt acgaaaaaac cgttgctgtt 180
tactggtctg acgctagtgg cgactggaac aacaacggca acaacgttgc cgcttcttac 240
tctgagtcca tctccggcac cgactacgag tactgggact tcagcaccac cattggctct 300
ggcggcatca agcagtctta cctcaagtac accgttagcg gcaacaccta ctacgacaac 360
aacggctcta acaactacga cattaccgag accaccgcta ccaccaccac caccaccacc 420
accaccacca ccaccggctc taccgccacc acctctacca cctctggccc taccagcacc 480
acctctacca cccccggccc tacccctacc aacgttcccg acggcaacgt taccgtcacc 540
gagtgggtta acacccagtt cgctatttct tggcctaccc tcctgaagaa cgtcaaccct 600
agcggcaccg tcaagggctt cattgctgca tcactcagca ccaacaaccc tgactacttc 660
tactcttgga cccgagacag cgctctcgtt gctcacacca tgacctacct gtacaacacc 720
tctgaggctg gcgacagcac cattgagtct gccctgaagg actacgttac cttcagcatt 780
aacgctatga acgctgctac cgtttgcaac tgcctgggcg agcctaagtt caacaccgac 840
ggctccggct acaccggcgc ttggggccgc ccccagaacg acggccccgc aagtcgagct 900
accaccatga ttctgttcgc cgactccttc ctcgcccagg gcggcgatgt ttcttacgtt 960
attaacaccc tcaagcctgc tatttacaag gacctggact acgtcgttag cacctggtct 1020
aacacctgct acgacctgtg ggaggaggtt aacggcgttc acatttacac cctgtctgtt 1080
atgcgccgcg ccctgattga cggcgctaac ttcgcccagc gaaacggcga cacctcccgc 1140
gtcagcggct acaccagcac cgccaccacc atcaagaccc gactggagtc tttctggagc 1200
gactctaaca actacattac cgttacccag tcttactccg gcggcgtcca gaaggccggc 1260
ctggacgtca gcaccctgat tgctgccaac attggcagcg ttggcgacgg cttctacacc 1320
cccggctctg acaaggtcct cgccaccgcc gtcgccatcg agaagtcctt cgctaacctg 1380
tacaccatta accagaacaa gccttcttgg ctgggcaacg ccattggccg ataccccgag 1440
gacacctacg acggctacgg caactccaag ggcaaccctt ggttcattgc taccgctacc 1500
tacgctgagc tgtactaccg agctatccta gagtggcagc agcaggcttc cgttaccgtt 1560
aactctatta acctgggctt cttctccaag ttcgacagct ctgcctctgt tggcaccgtt 1620
tacacccccg gcaccgactc tttcgccaac atggtttcta acgttgcttt cgctgctgac 1680
gagttcctgt ctaccatcga ctaccacgcc atgaacaacg gctctatgca cgagcagtac 1740
aaccgagaca ccggcatctc ccagggcgca cgcgacctga cctggtcaca cgctgctttc 1800
attaccgctg ccaaggctaa gctgggcgct cccgctttct aataa 1845
<210> 35
<211> 1818
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 35
atgaagctca tgaactctag catgaagacc tgcgttttct tcatcctgtc ttacttcagc 60
ctcctcgtca gcagcgctgc cgttcctacc tctgctgctg ttcaggttga gtcctacaag 120
tacgacggca ccaccttcag cggccgaatt ttcgtcaaga acattgctta ctccaaggtt 180
gttaccgtca tatatagcga cggctctgac aactggaaca acaacaacaa caagatcagc 240
gctgcttaca gcgaggctat tagcggctcc aactacgagt actggacctt ctctgctaag 300
ctgtccggca ttaagcagtt ctacgtcaag tacgaggttt ctggctctac ctactacgac 360
aacaacggca ccaagaacta ccaggtccag gctacctctg ccaccagcac caccgccacc 420
gccaccacca ccacctctac cagcaccacc accaccagca ccggccctac cagcaccgca 480
agtgtttctt tccctaccgg caactccacc attagctctt ggattaagaa ccaagaagaa 540
atttcccgat tcgccatgct gcgaaacatt aacccccccg gctccgccac cggcttcatt 600
gctgcctctc tgagcaccgc gggacccgac tactactact cttggacccg agacagcgcc 660
ctgaccgcta acgttattgc ttacgagtac aacaccacct tcgctggcaa caccaccctc 720
ctgaagtacc tgaaggacta cgttaccttc tccgtcaagt ctcagtctgt tagcaccgtt 780
tgcaactgcc tgggcgagcc taagttcaac gccgacggca gctcttacac cggcccttgg 840
ggccgacccc agaacgacgg ccccgccgaa cgggccgtca ccttcatgct cattgctgac 900
tcttacctga cccagaccaa ggacgcttct tacgtcaccg gcaccctgaa gcccgccatt 960
ttcaaggacc tcgactacgt tgtttccgtt tggtctaacg gctgctacga cctgtgggaa 1020
gaagttaacg gcgttcactt ctacaccctg atggttatgc gaaagggcct catcctgggc 1080
gctgacttcg ctgctcgaaa cggcgactct agccgagcct ctacctacaa gaacaccgcg 1140
agcaccatgg agtccaagat ctctagcttc tggtccgact ctaacaacta cgtccaggtt 1200
tcccagtctg ttaccgctgg cgtctccaag aagggcctcg acgttagcac cctcctcgcc 1260
gctaacattg gctccctgcc cgacggcttc ttcacccccg gcagcgagaa gattctggct 1320
accgccgttg ccctagaaaa cgctttcgca tctctgtacc ctattaactc taacctgcct 1380
tcttacctgg gcaactccat tggccgatac cccgaggaca cctacaacgg caacggcaac 1440
tcccagggca acccttggtt cctcgccgtt aacgcttacg ctgagctgta ctaccgagct 1500
attaaagagt ggatctctaa cggcaaggtt accgtctcta acatcagcct ccctttcttc 1560
aagaagttcg acagctccgc cacctctggc aagacctaca ccgctggcac cagcgacttc 1620
aacaacctcg ctcagaacat tgccctgggc gctgaccgat tcctgtctac cgttaagttc 1680
cacgcttaca acaacggcag cctgtctgaa gagtacgacc gatctaccgg catgtctacc 1740
ggcgcacgcg acctgacctg gtctcacgcc tcgctcatca ccgccgctta cgccaaggct 1800
ggcagccccg ctgcctaa 1818
<210> 36
<211> 1413
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 36
atgaagtctc cttacagcat gaaaaccgtc ctcgctctcc tgtccctgac ccctctgttc 60
accagcgacg ttgttgccgt tcctagcggc aactccacca tcaccgcttg gggctccaag 120
caggacggca tctctttcag caccatgctg ggcaacatca accctcccgg cagctctaag 180
ggcttcattg ctgcctcgct gtctaccgct ggccctaact actactactc ttggacccga 240
gacagcgctc tcgtcgcccg cgccattacc tacaagtact ccacctctta ccagaacgac 300
cccaagattc tgggcctcct gaaggactac gttacctacc aggttaacga gcagaccgag 360
tctaccgttt gcaactgcct cggcgagcct aagttcaacc ccgacggctc tagcttcagc 420
ggcccttggg gccgccctca gaacgacggc cctgctgagc gcgcttccac catgattctg 480
ttcgccaagt cttactacgc ccagaccaac gacgttggct acgtttctaa caccctgaag 540
cccgctattt acaaggacct ggactacatc gttaacgttt ggggcaacaa ctgcttcgac 600
ctgtgggagg aggttaacgg cgtccacttc tacaccctca tgatgatgcg acacggcctc 660
gtccagggca gcattttcgc taacaccctc ggcgacagca cccgagctaa cacctacaag 720
accgccgctc agaacattaa gaaccgaatt gacaccttct gggagtctgg ctctaactac 780
attgttgtta cccagaacca gtctgctggc gttaacaagc cttccggcct cgacgttgcc 840
gtcctgctgg ctgccaacca gggcggcctg ggcgacggcg tttacacccc cggctctgac 900
aaggtcctcg ccaccgccgt cgctctggag aagtctttcg caagcctgta ccccattaac 960
aagaacctgc cttcttacta cggcaccgcc attggccgat accccgagga cacctacaac 1020
ggcaacggca acagcgaggc taacccttgg ttcattgcca ccaccaccta cgctgagctg 1080
tactaccgag ctatttccga gtggaccaac ggctccggcg tcaccgtcaa ctctattaac 1140
aaggagttct tctctaagtt cgacgcctct gccaccaacg gcaaggttta cacccccggc 1200
agcgactctt tcaactccct ggtcaacaac gttgctattg ctgctgacaa cttcctcagc 1260
accgtccgat accaccagac ctccaacggc agcctgtctg agcagttcaa ccgatacacc 1320
ggcttcatga ccggcgcacg ggacctgacc tggtcccacg ccgctatggt taccgctctc 1380
gccgctaagg ctggcacccc tagcccttaa taa 1413
<210> 37
<211> 1830
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 37
atgcagctga acctgtactg gaagagcctc gccgtcctcg ctctgattca gatggttatg 60
gctgctaccg tccctaccac ccaggtccag ctcgactact acacctactc taacaacgtc 120
ctgtctggcc gaatctacgt tcagaacatt gcttacagca aggttgttaa ggttatctat 180
agcgacgcct ccggcaactg gaacaacaac ggcaacacca tctccgcttc ttacgtcgag 240
tctatttccg gcaccaacta cgagtactgg gacttcagcg ccaccattgg caccgctggc 300
atcaagcagt tctacctgcg atacgacgtt tccggctcca cctactacga caacaacggc 360
ggcgacaaca acaactacaa cgttgttgct accacctcta ccacctctag ctctaccacc 420
accaccaccg ctaccaccac caccgccacc aagaccacca gcaccacctc tgccaccgct 480
acccctacct ctagcgccac cttccctagc ggcaactcta ccattaccac ctgggccaag 540
tctcagaagg acatttcttg gaagaccctc ctgaccagcc tgaaccctag cggcaccgcc 600
aagggcttca ttgctgcgag tctgtctacc tctaaccctg actactacta cgcttggacc 660
cgagacagcg ctctcgttgc ccgaaccatg gttaacatgt acaacaccac cgaggctggc 720
tctgcttcag tcctgggcct cctgcaggac tacgttacct tccagattaa cgccatgagc 780
accagcaccg tttgcaactg cctgggcgag cctaagttca accccgacgg cagctcttac 840
accggcgctt ggggccgccc ccagaacgac ggccccgctg aacgtgcaag caccttcatc 900
ctgttcgctg actcttacat tgcccagggc ggccagctgt cttacgtcac cggcaccctc 960
gctcccgcca tctacaagga cctgaactac gtcgtttcta cctggtctaa caactgcttc 1020
gacctgtggg aagaggttaa cggccgacac atgttcaccc tcgccgtcat gcgacgcgcc 1080
ctcctggacg gcgttaactt cgcctcccga attggcgaca ccacctactc tagcacctgg 1140
agctctaccg cttctagcat ccagtctacc ctgtctggct actacctgtc ttccggcaac 1200
tacattacca ccgttcagaa cttccagtct ggcgtttcca aggctggcta cgacgtcagc 1260
accctcatcg ctgctaacgt tgcgggcatg ggcgacggct tcttcacccc cggctctgac 1320
gaggttctcg ctaccgccgt tgctattgag aacaagttct ctagcctgta cggcgtcaac 1380
gctaacaagg cttcttacct cggcaccgcc attggccgat accccgagga cacctacaac 1440
ggctacggca acggccaggg caacccttgg ttcattgcta ccgctgctta cgccgagctg 1500
tactaccgag ctattctgga gtggcagacc aagagcagca tcgttgttaa ctccaagaac 1560
ctcggcttct tctccaagtt cgactcttct gccgccgttg gcaccaccta cacccccggc 1620
accaccgctt actctaacat ggtccagaac gtcgccctcg ctgctgaccg attcctcagc 1680
accgtccagc tgcacgctgc caccaacggc tccatgtctg agcagttcaa ccgagacacc 1740
ggcgttatgc agggagcccg tgacctgacc tggtctcaca gcgctttcat taccgctgcc 1800
cgagctaagc tgggcagccc cgtttactaa 1830
<210> 38
<211> 1404
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 38
atgcgagcta ccaccgctat tctgtctctg ttcaccaccg cctctatggc tctggctgcc 60
aactctaccg tccctgacgg caactccacc accaccgctt gggtcaagaa gcaagaagcc 120
atctcttgga ccgacctgaa gaccaacgtc aaccctgagg gcgctgctaa gggcttcatt 180
gctgcctctc tgtctaccag cgagcctgac tactactacg cttggacccg agactctgcc 240
ctcgttgctc gcgttatggt taacaagtac aacaccaccg acgctgggga cgccaacctc 300
ctcggcctcc tgcaggacta cgtttctttc cagatcaacg caatgggcga gtctaccgtt 360
tgcaactgcc tgggcgagcc taagttcaac cccgacggca gctcttacac cggcgcttgg 420
ggccgccccc agaacgacgg ccccgccgag cgagcttcta ccttcattct cctcgccgac 480
agcatgattg cccagaagtc tgctaacggc tcttacgtca gcgacaccct ggctcccgct 540
atctacaagg acctggctta cgttgcaagc acctgggaga acgcttgcta cgacctgtgg 600
gaagaggtta acggcaagca catgtacacc ctcagcgtca tgcgacgagc cctcctggac 660
ggcgccgact tcgcttctcg gcagggccag accgccaacg ttacctcttg gaagtctacc 720
gctgacaaga ttaagtctag cctggagggc ttcttcagct ccgacaacgg ctacattgag 780
gttacccagg acatgcaggg cggcgtccag aagaagggcc tggacgtttc caccctcatc 840
gctgctaaca ttggcagcat gggcgacggc ttctacaccc ccggcagcga cgaggttctc 900
gccaccgctg ttgccgttga ggctgccttc gctgacctgt acaagattaa ccagaacacc 960
aactccaccg gcctcggcac cgccatcggc cgataccccg aggacaccta caacggcgtt 1020
ggcaactccc agggcaaccc ttggttcatt gctaccaaca ccttcgctga gctgtactac 1080
cgcgccatcc tggagtggca gaacaagggc accattaccg ttaactccgt taacgctgct 1140
ttcttcagca agtttgactc tagcgctaag gccggtacaa cctacaagtc tggctctacc 1200
gagtttgata gcctcattaa caaggttgct ctggctgctg acgctttcct gaacaccgtc 1260
cagacctacg ctgcttctaa cggctctatg tctgagcagt acaaccgaga caccggcgcc 1320
ctgaccggcg cccgagacct gacctggtct cacgcaagtc tcattaccgc cgctaacgcc 1380
aagctcggca ccccttacaa ctaa 1404
<210> 39
<211> 1419
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 39
atgaagctcg gccgaattgg cttctctatt ctgtctgttg cctccgtttt ctctcaggcc 60
gtcgttaccg ctgccgctgc cgcctctgtc cctagcggca acgccaccat tacctcttgg 120
attcaggagc agctcgctat ttcttggaac accatgctga ccagcgttaa ccccgttggc 180
gccgtcaccg gcttcatcgc tgcgagtctg tctaccgcta accccgacta ctactactgc 240
tggacccgag acgctgctct cgtcgcgaga gttatgacct tcatgtacaa caccacccag 300
gctggcgaca gcggcctcct gggcatcctc caggactacg tttccttcca gatccacgca 360
atgggcgagt ctaccgtttg caactgcctg ggcgagccca agttcaaccc cgacggcagc 420
tcttacaccg gcgcttgggg ccgccctcag aacgacggcc ctgctgagcg agcctctacc 480
ttcatcaaga ttgctgactc ttacctgacc cagaccggcg atgtatccta cgttaccaac 540
accctcaagc ccgctattta cgaggacctg gactacattg ttaacgtttg gcagaacacc 600
tgcttcgacc tgtgggagga ggtttacgga atgcacatgt acaccctggc tgttatgcga 660
cgaggcctcc tcgacggcgc cgacttcgct acccgaaacg gcgacaccga caaggcctct 720
acctacacca gcaccgccac cagcatccag acccgactgg ccaccttctg gtctgactcc 780
aacggctaca ttaccgttac ccaggactac cagtccggcg tcagcaaggc tggcctggac 840
gttagcaccc tgattgctgc taacgttgcc ggcatgaacg acggcttctt cacccctggc 900
tctgacgaga ttctcgctac cgccgtcaag gttgaggctg ctttcgctaa cctgtacggc 960
attaacatta acaaggcctc ttacctgggc aacgccattg gccgataccc cgaggacacc 1020
tacaacggca acggcaactc tgagggcaac ccttggttca ttgccaccgc tgctttcgct 1080
gagctgtact accgcgccat tatggagtgg cagctccgag gcagctctat taccgtcaac 1140
tctgttaacc agggcttctt caccaagttc gaccctagcg ccaccgccgg caccacctac 1200
acccccggca ccgacgcttt caactccctc attgacaacg ttgccctcgc tgctgaccag 1260
ttcttcagca ccatccacct ccaccgcgcc accaacggct ctatgtctga gcagtacaac 1320
cgagacaccg gcttcatgca gggcgctcgg gacctgacct ggtcccacgc cgctttcatc 1380
accgccgcta aggctaagca gggcacccct agcttctaa 1419
<210> 40
<211> 1842
<212> DNA
<213> 人工序列
<220>
<223> .
<400> 40
atgcgccccg ttcacctgtg gaagagcctg ctcctcgccg ctgctaccgc tgttaccggc 60
accatcgccg ttgatgttcc ttctgttcct atccagctag agtcttacac ctactctgag 120
aacgttttcg ccggccgaat tttcgtccag aacattgctt acaccaagga ggttaacgtt 180
ttctggtctg acgcttccga cgactggaac gacaacggca actccgttgc tgcttcttac 240
tccgagtcta ttgctgacac caactacgag tactgggagt tcagcaccac cattggctct 300
gccggcatct cccagtctta cctgcgatac gatgtgtctg gctctattta ctacgacaac 360
aacgactccc agaactacga cattaccgag accagcaccc ctaccaccac caccgctgcc 420
cctacctcta ccacctctac ccctaccacc accaccgacg gcggcagcac caccaccacc 480
accgccacct ccgtccctac ctctaccggc gtccctgagg gcaacgctac cattagcgag 540
tgggcatctg cccagctgga catctcttgg cctaacctca tgatgaacgt taaccctagc 600
ggcgctgtta ccggctccat tgttgcctca ctgtctacct ctaaccctga ctacttctac 660
atttggaccc gagacgctgc gatggttgcc cgcgttatgg tttacatgta caacaccacc 720
gaggctggcg acaccaacct gctcaacgcc ctgaccgact acgttacctt ctctatttcc 780
agcatgaacg ttgacaccgt ttgcgactgc ctgggcgagc ctaagttcaa cgttgacggc 840
tctggctaca ccggcgcttg gggccgcccc cagaacgacg gccccgccga gcgcgcctct 900
accatgatcc tgattgctga ctcttacatc gcccagggcg gcgatgtatc ttacgtcacc 960
gacaccctca agcctgctat ttacaccgac ctggactacg tcgttgacac ctggtctaac 1020
gtttgcttcg acctgtggga ggaggttaac ggcattcaca tgtacaccct cagcgtcatg 1080
cgaaaggccc tcctggacgg cgccaacttc gctacccgaa acggcgacac ctcccgcgtt 1140
agcggctacg agagcaccgc ttcatccatc aagacccgac tggagtcttt ctggtctagc 1200
tctaacaact acattaccgt cacccagtcc ttcagcggcg gcgtttccaa ggccggcctg 1260
gacgtcagca ccctgattgc tgccaacctc gcttccatga acgacggctt ctacaccccc 1320
ggctctgacg agattctcgc taccgccgtt gctattgaga actctttcat ctccgagtac 1380
accctcaacc agaaccgccc ttcttggctg tccaccgcca ttggccgata ccctgaggac 1440
tcttacgacg gctacggcaa ctcccagggc aacccttggt tcatcgctac cgctacctac 1500
gctgagctgt actaccgagc tatcctagag tggcagcagc aggcttcaat tagcgttaac 1560
agcgttaacc tgggcttctt cagcaagttc gactctgacg cttctgttgg caccgtttac 1620
acccccggca ccgaggactt cgccaacatg gtttctaacg tcgctttcgc tgctgacgag 1680
ttcctcgcca ccattgagaa ccacagcgcc gtcaacggca gcctcagcga gcagtacaac 1740
cgagacaccg gcattatgca gggcgcaaga gacctgacct ggtcacacgc tgccttcatt 1800
accgctgcca aggccaagca gggcgctcct attcactaat aa 1842
<210> 41
<211> 19
<212> PRT
<213> 瓶霉（Saksenaea vasiformis）
<400> 41
Met Pro Ser Trp Lys Thr Leu Phe Leu Leu Leu Gly Pro Ile Ala Thr
1 5 10 15
Ala Ala Ala
<210> 42
<211> 23
<212> PRT
<213> 螺旋巴克斯霉（Backusella circina）
<400> 42
Met Lys Leu Phe Gly Thr Leu Lys Ala Ser Phe Val Phe Leu Ile Gly
1 5 10 15
Cys Ile Val Phe Ala Met Ala
20
<210> 43
<211> 24
<212> PRT
<213> 螺旋巴克斯霉（Backusella circina）
<400> 43
Met Lys Pro Phe Gly Pro Ile Lys Thr Thr Leu Phe Ile Ile Ile Ser
1 5 10 15
His Phe Ser Leu Asn Ser Leu Ala
20
<210> 44
<211> 24
<212> PRT
<213> Benjaminiella poitrasii
<400> 44
Met Lys Phe Ser His Leu Phe Arg Lys Pro Thr Leu Leu Ile Val Thr
1 5 10 15
Phe Leu Thr Ala Thr Val Phe Ala
20
<210> 45
<211> 24
<212> PRT
<213> 瓜笄霉（Choanephora cucurbitarum）
<400> 45
Met Val Ser Phe Asn Phe Leu Lys Lys Pro Val Phe Ile Val Ile Thr
1 5 10 15
Cys Leu Thr Val Ser Val Cys Ala
20
<210> 46
<211> 25
<212> PRT
<213> 匍枝根霉（Rhizopus stolonifer）
<400> 46
Met Lys Leu Phe Gly Leu Pro Leu Lys Pro Ser Leu Phe Phe Val Val
1 5 10 15
Ser Tyr Leu Ser Phe Leu Ala Ser Ala
20 25
<210> 47
<211> 24
<212> PRT
<213> 卷枝毛霉（Mucor circinelloides）
<400> 47
Met Lys Phe Ser Asn Leu Leu Lys Lys Pro Leu Leu Leu Ile Ala Gly
1 5 10 15
Ile Leu Ala Ala Thr Val Val Ala
20
<210> 48
<211> 21
<212> PRT
<213> Dichotomocladium elegans
<400> 48
Met Lys Leu Asn Ser Val Trp Lys Ala Phe Ser Ile Leu Leu Met Ala
1 5 10 15
Thr Met Ala Phe Ala
20
<210> 49
<211> 23
<212> PRT
<213> Fennellomyces属物种
<400> 49
Met Arg Pro Ser Leu Val Trp Lys Ser Leu Leu Met Val Val Leu Thr
1 5 10 15
Ala Ala Met Gly Thr Tyr Ala
20
<210> 50
<211> 24
<212> PRT
<213> 桃吉尔霉（Gilbertella persicaria）
<400> 50
Met Val Phe Ser Lys Leu Phe Lys Lys Pro Ile Leu Leu Ile Val Thr
1 5 10 15
Tyr Leu Thr Val Thr Val Val Ala
20
<210> 51
<211> 24
<212> PRT
<213> 卷枝毛霉（Mucor circinelloides）
<400> 51
Met Lys Phe Ser Asn Leu Leu Lys Lys Pro Leu Leu Leu Ile Ala Gly
1 5 10 15
Ile Leu Ala Val Thr Val Val Ala
20
<210> 52
<211> 25
<212> PRT
<213> 伞形卷霉（Circinella umbellata）
<400> 52
Met Met Pro Asn His Ile Trp Lys Cys Leu Phe Leu Ala Met Thr Thr
1 5 10 15
Val Phe Met Thr Val Leu Val Gln Gly
20 25
<210> 53
<211> 24
<212> PRT
<213> Mucor cordense
<400> 53
Met Lys Phe Ser Lys Leu Phe Thr Lys Pro Thr Leu Phe Ile Val Ser
1 5 10 15
Ile Ile Ile Ser Val Ala Ser Ser
20
<210> 54
<211> 23
<212> PRT
<213> Phascolomyces articulosus
<400> 54
Met Lys Pro Ile His Leu Trp His Tyr Leu Phe Leu Ala Met Thr Ala
1 5 10 15
Ala Phe Met Gly Val Leu Ala
20
<210> 55
<211> 27
<212> PRT
<213> 小孢根霉（Rhizopus microsporus）
<400> 55
Met Lys Leu Met Asn Ser Ser Met Lys Thr Cys Val Phe Phe Ile Leu
1 5 10 15
Ser Tyr Phe Ser Leu Leu Val Ser Ser Ala Ala
20 25
<210> 56
<211> 26
<212> PRT
<213> 伞菌霉（Spinellus fusiger）
<400> 56
Met Lys Ser Pro Tyr Ser Met Lys Thr Val Leu Ala Leu Leu Ser Leu
1 5 10 15
Thr Pro Leu Phe Thr Ser Asp Val Val Ala
20 25
<210> 57
<211> 21
<212> PRT
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 57
Met Gln Leu Asn Leu Tyr Trp Lys Ser Leu Ala Val Leu Ala Leu Ile
1 5 10 15
Gln Met Val Met Ala
20
<210> 58
<211> 19
<212> PRT
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 58
Met Arg Ala Thr Thr Ala Ile Leu Ser Leu Phe Thr Thr Ala Ser Met
1 5 10 15
Ala Leu Ala
<210> 59
<211> 20
<212> PRT
<213> Thermomucor属物种
<400> 59
Met Lys Leu Gly Arg Ile Gly Phe Ser Ile Leu Ser Val Ala Ser Val
1 5 10 15
Phe Ser Gln Ala
20
<210> 60
<211> 23
<212> PRT
<213> Zychaea mexicana
<400> 60
Met Arg Pro Val His Leu Trp Lys Ser Leu Leu Leu Ala Ala Ala Thr
1 5 10 15
Ala Val Thr Gly Thr Ile Ala
20
<210> 61
<211> 449
<212> PRT
<213> 瓶霉（Saksenaea vasiformis）
<400> 61
Ala Pro Val Asp Lys Gln Ser Leu Pro Thr Gly Asn Ser Thr Ile Ser
1 5 10 15
Ser Trp Val Ser Lys Gln Glu Asp Ile Ser Phe Ser Glu Met Leu Arg
20 25 30
Asn Val Asn Pro Glu Gly Thr Ala Lys Gly Phe Val Ala Ala Ser Leu
35 40 45
Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ala Ala
50 55 60
Leu Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr Asn Ala Gly
65 70 75 80
Asp Ser Lys Ile His Gly Val Leu Asp Asp Tyr Val Asn Phe Gln Ile
85 90 95
Asn Thr Gln Ser Glu Ser Thr Pro Cys Asn Cys Leu Gly Glu Pro Lys
100 105 110
Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln
115 120 125
Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Phe Met Leu Ile Ala Asp
130 135 140
Ser Phe Leu Ser Gln Thr Lys Asn Ala Ser Tyr Phe Thr Asn Thr Leu
145 150 155 160
Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asp Thr Trp Ser
165 170 175
Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr
180 185 190
Thr Leu Met Val Met Arg Arg Ser Leu Leu Asp Gly Ala Asn Phe Ala
195 200 205
Thr Arg Asn Gly Asp Asn Ser Lys Ala Ser Thr Tyr Ser Gly Val Ala
210 215 220
Ala Lys Ile Gln Ala Arg Leu Asn Ser Phe Trp Asp Ala Gly Lys Asn
225 230 235 240
Tyr Ile Thr Val Thr Gln Asp Tyr Lys Asn Gly Val Glu Lys Pro Ser
245 250 255
Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Gly
260 265 270
Asp Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala Thr Ala Leu
275 280 285
Ala Phe Glu Lys Ser Met Ala Ser Leu Tyr Pro Leu Asn Asn Asn Leu
290 295 300
Pro Ser His Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
305 310 315 320
Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr
325 330 335
Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Lys Asn Thr
340 345 350
Gly Val Thr Val Thr Pro Ile Ser Lys Asp Phe Phe Val Arg Phe Asp
355 360 365
Ser Ser Ala Ala Pro Gly Lys Lys Tyr Asn Pro Gly Ser Gln Glu Phe
370 375 380
Ala Thr Leu Thr Gln Ser Ile Ala Ala Ala Ala Asp Arg Phe Met Ser
385 390 395 400
Thr Val Gln Tyr His Gln Asn Pro Asn Gly Ser Leu Ser Glu Glu Phe
405 410 415
Asp Arg Ser Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
420 425 430
His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly Ser Pro Ser
435 440 445
Phe
<210> 62
<211> 582
<212> PRT
<213> 螺旋巴克斯霉（Backusella circina）
<400> 62
Asp Thr Val Pro Thr Thr Gln Val Lys Leu Gln Ser Tyr Thr Tyr Ser
1 5 10 15
Gly Gly Val Leu Ser Gly Thr Ile Tyr Val Gln Asn Ile Asp Tyr Thr
20 25 30
Lys Val Val Thr Val Ile Tyr Ser Asp Gly Ser Gly Asn Trp Asn Asn
35 40 45
Asn Gly Asn Thr Ile Ser Ala Ser Tyr Ser Ser Ser Ile Ser Gly Thr
50 55 60
Asn Tyr Glu Tyr Trp Thr Phe Ser Ala Ser Val Ser Ser Ile Gln Gln
65 70 75 80
Phe Tyr Ile Ser Tyr Leu Val Asp Gly Thr Thr Tyr Tyr Asp Asn Asn
85 90 95
Gly Gly Tyr Gly Ala Asn Tyr Ala Val Thr Ala Thr Thr Thr Thr Ala
100 105 110
Ser Ser Thr Thr Lys Thr Thr Ser Gly Thr Thr Lys Thr Ser Thr Thr
115 120 125
Ser Thr Ala Thr Ala Thr Ser Thr Gly Thr Ser Ser Phe Pro Ser Gly
130 135 140
Asn Ser Thr Ile Ser Thr Trp Ser Pro Ser Gln His Ser Ile Ser Leu
145 150 155 160
Tyr Ala Met Leu Arg Asn Ile Asn Pro Pro Gly Ser Ala Ala Gly Phe
165 170 175
Ile Ser Ala Ser Leu Ser Thr Ser Gly Pro Asp Tyr Tyr Tyr Ser Trp
180 185 190
Thr Arg Asp Ser Ala Leu Val Ala His Val Ile Val Asn Glu Tyr Asn
195 200 205
Thr Thr Tyr Gln Gly Asn Ser Thr Leu Leu Gly Ile Leu Lys Asp Tyr
210 215 220
Val Thr Tyr Ser Leu Asn Ala Gln Thr Thr Ser Thr Val Cys Asn Cys
225 230 235 240
Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Ala
245 250 255
Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Val Ser Phe
260 265 270
Ile Tyr Phe Ala Asp Ser Tyr Leu Thr Gln Thr Ser Asp Ser Ser Tyr
275 280 285
Val Thr Gly Thr Leu Ala Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val
290 295 300
Val Ser Val Trp Ser Asn Gly Cys Phe Asp Leu Trp Glu Glu Val Asn
305 310 315 320
Gly Ile His Phe Tyr Thr Leu Met Phe Met Arg Arg Gly Leu Leu Asp
325 330 335
Gly Ala Asn Phe Ala Ser Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr
340 345 350
Tyr Thr Ser Thr Ala Ala Ser Ile Lys Thr Lys Ile Asp Gly Phe Trp
355 360 365
Val Ser Ser Gly Asn Tyr Val Gln Val Ser Gln Ser Val Thr Ser Gly
370 375 380
Val Ser Lys Ala Gly Tyr Asp Ala Ser Thr Leu Ile Ala Ala Asn Gln
385 390 395 400
Ala Ser Arg Gly Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu
405 410 415
Ala Thr Ala Val Ala Ile Glu Ser Gln Phe Ser Ser Leu Tyr Ser Ile
420 425 430
Asn Thr Asn Lys Ala Ser Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro
435 440 445
Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe
450 455 460
Leu Cys Thr Asn Ala Phe Gly Glu Leu Phe Tyr Arg Ala Ile Ser Glu
465 470 475 480
Trp Asn Ala Ala Gly Ser Val Thr Val Asn Ser Val Asn Leu Ala Phe
485 490 495
Phe Lys Lys Tyr Asp Ser Ser Thr Ser Ser Gly Thr Thr Tyr Thr Val
500 505 510
Gly Thr Ser Ala Tyr Asn Asn Leu Val Gln Asn Val Ala Leu Ala Ala
515 520 525
Asp Ala Tyr Phe Ser Thr Val Lys Tyr His Ala Leu Thr Asn Gly Ser
530 535 540
Met Ser Glu Gln Tyr Asp Arg Ser Ser Gly Met Ala Thr Gly Ala Arg
545 550 555 560
Asp Leu Thr Trp Ser His Ala Ala Met Ile Thr Ala Leu Lys Ala Lys
565 570 575
Ser Gly Thr Pro Val Tyr
580
<210> 63
<211> 578
<212> PRT
<213> 螺旋巴克斯霉（Backusella circina）
<400> 63
Asp Thr Val Pro Thr Thr Gln Val Glu Val Lys Thr Tyr Thr Tyr Ser
1 5 10 15
Gly Gly Glu Leu Ser Gly Thr Ile Tyr Val Glu Asn Ile Asp Tyr Thr
20 25 30
Lys Val Val Thr Val Ile Tyr Ala Asp Gly Ser Asp Asp Trp Asn Asn
35 40 45
Asn Gly Asn Thr Ile Ala Ala Ala Phe Ser Glu Ser Ile Ser Asp Thr
50 55 60
Asn Tyr Glu Tyr Trp Thr Phe Ser Ser Ser Val Asn Ser Ile Lys Glu
65 70 75 80
Phe Tyr Val Lys Tyr Asp Val Asp Gly Asn Thr Tyr Tyr Asp Asn Asn
85 90 95
Gly Asn Ala Asn Tyr Gln Val Ser Ala Ser Ser Thr Thr Thr Thr Thr
100 105 110
Ala Thr Lys Thr Thr Thr Thr Ala Thr Lys Thr Thr Ala Thr Ala Thr
115 120 125
Ser Thr Ala Thr Gly Ser Thr Ser Phe Pro Ser Gly Asn Ser Thr Ile
130 135 140
Ser Ser Trp Ile Lys Gly Gln Glu Asp Thr Ser Arg Ser Val Met Leu
145 150 155 160
Gly Asn Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe Ile Ser Ala Ser
165 170 175
Leu Ser Thr Ser Gly Pro Asp Tyr Tyr Tyr His Trp Thr Arg Asp Ala
180 185 190
Ala Leu Val Ala His Val Ile Val Asn Asp Tyr Asn Thr Thr Leu Ser
195 200 205
Gly Asp Ser Ser Thr Leu Gln Val Ile Lys Asp Tyr Val Thr Phe Ser
210 215 220
Val Asn Ser Gln Ser Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro
225 230 235 240
Lys Phe Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro
245 250 255
Gln Asn Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe Ile Phe Phe Ala
260 265 270
Asp Thr Tyr Leu Ala Gln Gly Gly Asp Ser Ser Tyr Val Thr Gly Thr
275 280 285
Leu Ala Pro Ala Ile Tyr Ala Asp Leu Asp Tyr Val Val Asn Asn Trp
290 295 300
Ser Thr Gly Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe
305 310 315 320
Tyr Thr Leu Met Val Met Arg Arg Gly Leu Ile Asp Gly Ala Ser Phe
325 330 335
Ala Ser Arg Asn Gly Asp Ser Thr Arg Ser Ser Ser Tyr Thr Ser Thr
340 345 350
Ala Lys Ser Ile Ala Thr Lys Ile Asp Ser Phe Trp Ser Ala Ser Asn
355 360 365
Asn Tyr Val Ala Val Ser Gln Ser Val Thr Ser Gly Val Ser Lys Ala
370 375 380
Gly Tyr Asp Ala Ser Thr Ile Ile Ala Ala Asn Gln Ala Ser Leu Gly
385 390 395 400
Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ser Val
405 410 415
Ala Val Glu Asn Ala Phe Ser Ser Leu Tyr Thr Val Asn Thr Gly Lys
420 425 430
Ala Ser Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
435 440 445
Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Tyr Leu Cys Thr Asn
450 455 460
Ala Leu Gly Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Asn Ala Ala
465 470 475 480
Gly Ser Val Thr Val Asn Ser Val Asn Leu Gly Phe Phe Gln Lys Ile
485 490 495
Asp Ser Ser Ile Ser Ser Gly Thr Thr Phe Thr Leu Gly Thr Ser Asp
500 505 510
Tyr Ser Thr Leu Val Asp Asn Val Ala Leu Ala Ala Asp Lys Phe Phe
515 520 525
Ala Val Val Gln Tyr His Glu Arg Ser Asn Gly Ser Met Pro Glu Gln
530 535 540
Phe Gly Arg Glu Asp Gly Leu Pro Thr Gly Ala Arg Asp Leu Thr Trp
545 550 555 560
Ser His Ala Ala Met Ile Ser Ala Ala Arg Ala Lys Ala Gly Thr Pro
565 570 575
Val Tyr
<210> 64
<211> 577
<212> PRT
<213> Benjaminiella poitrasii
<400> 64
Glu Thr Val Pro Thr Thr Ser Gln Val Lys Leu Lys Ser Tyr Thr Tyr
1 5 10 15
Asp Gly Ser Thr Leu Ser Gly Gln Ile Tyr Ile Gln Asn Ile Ala Tyr
20 25 30
Thr Lys Val Val Thr Val Ile Tyr Ser Asp Ala Ser Ser Asn Trp Asn
35 40 45
Asn Asn Gly Asn Thr Ile Ala Ala Ser Tyr Thr Asn Gly Ile Ser Gly
50 55 60
Thr Asn Tyr Glu Tyr Trp Asn Phe Thr Gly Pro Val Ser Ser Ile Asn
65 70 75 80
Ala Phe Tyr Ile Lys Tyr Asp Val Ser Gly Asn Thr Tyr Tyr Asp Asn
85 90 95
Asn Asn Asn Ala Asn Tyr Gln Val Thr Lys Thr Ser Thr Thr Thr Thr
100 105 110
Thr Ser Thr Ala Thr Ala Thr Thr Thr Thr Lys Thr Ser Thr Pro Thr
115 120 125
Ser Thr Gly Val Pro Ser Asn Phe Pro Thr Gly Asn Ser Thr Ile Ser
130 135 140
Thr Trp Leu Lys Ser Gln Ile Gly Ile Ser Arg Tyr Ala Met Leu Arg
145 150 155 160
Asn Ile Asn Pro Ala Gly Ser Ala Val Gly Phe Ile Ala Ala Ser Leu
165 170 175
Ser Thr Ala Asn Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala
180 185 190
Leu Thr Ser Tyr Val Ile Ala Asn Asp Tyr Asn Ser Thr Leu Ala Gly
195 200 205
Asn Ser Thr Ile Leu Gln Ile Met Lys Asp Tyr Val Thr Phe Ser Val
210 215 220
Lys Ser Gln Ser Val Ser Thr Ala Cys Asn Cys Leu Gly Glu Pro Lys
225 230 235 240
Phe Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln
245 250 255
Asn Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe Ile Leu Phe Ala Asp
260 265 270
Ser Tyr Leu Lys Gln Thr Gly Asp Ala Ser Tyr Val Thr Gly Thr Leu
275 280 285
Lys Pro Ala Ile Phe Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Thr
290 295 300
Asn Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr
305 310 315 320
Thr Leu Met Val Met Arg Lys Gly Leu Ile Arg Gly Ala Asn Phe Ala
325 330 335
Thr Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Thr Asn Thr Ala
340 345 350
Ala Ser Ile Lys Thr Lys Met Asp Ser Phe Trp Ser Ser Gly Asn Asn
355 360 365
Tyr Ile Ala Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly
370 375 380
Tyr Asp Val Ala Asn Ile Ile Ala Ala Asn Val Gly Ser Leu Gln Asp
385 390 395 400
Gly Val Tyr Thr Pro Gly Ser Asp Arg Ile Leu Ala Thr Ala Val Ala
405 410 415
Val Glu Ala Lys Phe Ala Ser Leu Tyr Gly Val Asn Ser Asn Leu Pro
420 425 430
Gly Tyr Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn
435 440 445
Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Asn Ala
450 455 460
Tyr Ala Glu Leu Tyr Tyr Arg Ala Ile Arg Glu Trp Tyr Asp Asn Gly
465 470 475 480
Gly Val Thr Val Asn Ser Val Asn Leu Pro Phe Phe Lys Lys Phe Asp
485 490 495
Ser Ser Ala Ala Ser Gly Thr Thr Tyr Thr Val Gly Thr Thr Ala Phe
500 505 510
Asn Thr Met Val Ser Asn Val Ala Ala Ala Ala Asp Lys Phe Phe Ser
515 520 525
Thr Val Lys Phe His Ala Tyr Thr Asn Gly Ser Met Ser Glu Gln Phe
530 535 540
Gly Arg Asn Asp Gly Leu Cys Thr Gly Ala Arg Asp Leu Thr Trp Ser
545 550 555 560
His Ala Ser Leu Ile Ser Ala Ala Leu Ala Lys Ala Gly Thr Pro Ser
565 570 575
Val
<210> 65
<211> 574
<212> PRT
<213> 瓜笄霉（Choanephora cucurbitarum）
<400> 65
Gln Ser Val Pro Thr Ser Asp Pro Val Lys Val Lys Thr Phe Ser Tyr
1 5 10 15
Asp Gly Asn Ser Phe Ser Gly Gln Ile Tyr Ile Lys Asn Ile Ala Tyr
20 25 30
Glu Lys Thr Val Thr Val Ile Tyr Ser Asp Gly Thr Gly Asn Trp Asn
35 40 45
Asn Asn Asn Asn Lys Ile Ala Ala Ala Phe Ser Glu Ala Ile Ser Gly
50 55 60
Ser Asn Tyr Glu Tyr Trp Thr Phe Ser Ala Ser Val Pro Ser Ile Lys
65 70 75 80
Gln Phe Tyr Val Lys Tyr Asp Val Ser Gly Lys Thr Tyr Tyr Asp Asn
85 90 95
Asn Gly Ser Lys Asp Tyr Asn Val Val Thr Ser Gly Pro Thr Thr Thr
100 105 110
Ser Ser Gly Pro Ser Lys Thr Thr Thr Asn Gly Pro Ala Pro Thr Ser
115 120 125
Thr Asn Thr Asn Phe Pro Ser Gly Asn Pro Ser Ile Thr Ser Trp Ile
130 135 140
Asp Lys Gln Ile Asp Ile Ser Arg Ser Ala Met Leu Lys Asn Ile Asn
145 150 155 160
Pro Ala Gly Thr Val Lys Gly Phe Ile Ala Ala Ser Leu Ser Thr Ser
165 170 175
Asn Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ala Ala Leu Val Ala
180 185 190
His Val Val Ala Asn Asp Tyr Asn Arg Thr Lys Ser Gly Asp Ala Thr
195 200 205
Tyr Leu Gly Leu Leu Lys Asp Tyr Val Thr Phe Ser Ile Asn Ser Gln
210 215 220
Asn Thr Pro Thr Ala Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Lys
225 230 235 240
Asp Gly Ser Gly Tyr Asn Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly
245 250 255
Pro Ala Glu Arg Ala Asp Thr Phe Val Leu Ile Ala Asp Ser Ile Leu
260 265 270
Thr Gln Thr Lys Asp Val Ser Tyr Val Thr Gly Thr Leu Arg Pro Ala
275 280 285
Ile Tyr Thr Asp Leu Asp Tyr Val Val Arg Thr Trp Ser Asn Gly Cys
290 295 300
Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met
305 310 315 320
Val Met Arg Arg Ala Leu Leu Val Gly Ala Asn Phe Ala Ser Arg Asn
325 330 335
Gly Asp Ser Ala Arg Ala Ser Asn Tyr Asn Asn Ala Ala Asn Ser Ile
340 345 350
Lys Ser Lys Ile Asp Ser Phe Trp Ser Ser Asn Asn Asn Tyr Val Ala
355 360 365
Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly Tyr Asp Val
370 375 380
Ser Thr Leu Ile Ala Ala Asn Val Gly Ser Leu Ser Asp Gly Phe Tyr
385 390 395 400
Thr Pro Gly Ser Glu Arg Met Leu Ala Thr Ala Val Ala Ile Glu Asp
405 410 415
Lys Phe Ala Asn Leu Tyr Gly Ile Asn Arg Asn Leu Asp Ser Ser Leu
420 425 430
Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly
435 440 445
Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Asn Ala Tyr Ala Glu
450 455 460
Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Asn Asn Asn Gly Gly Val Thr
465 470 475 480
Val Thr Asn Val Asn Leu Asn Phe Phe Lys Lys Phe Asp Gly Ser Ala
485 490 495
Ser Val Gly Thr Lys Tyr Thr Ala Gly Ser Ala Ala Tyr Asn Thr Leu
500 505 510
Thr Gln Asn Ile Ala Leu Ala Ala Asp Lys Phe Phe Asn Thr Val Lys
515 520 525
Val His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr His Arg Asp
530 535 540
Thr Gly Ser Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser
545 550 555 560
Leu Ile Thr Ala Ala Leu Ala Lys Ala Gly Thr Pro Val Ala
565 570
<210> 66
<211> 584
<212> PRT
<213> 匍枝根霉（Rhizopus stolonifer）
<400> 66
Ala Ser Ile Pro Ser Ser Ser Glu Val Gln Leu Asp Thr Tyr Ala Tyr
1 5 10 15
Asp Gly Ala Lys Phe Ser Gly Lys Ile Tyr Val Lys Asn Ile Ala Tyr
20 25 30
Glu Lys Thr Val Thr Val Val Tyr Ala Asp Gly Ser Asp Asn Trp Asn
35 40 45
Asn Asn Gly Asn Ile Ile Ser Ala Thr Phe Ser Thr Pro Ile Ser Gly
50 55 60
Ser Asn Tyr Glu Tyr Trp Thr Phe Ser Ser Ser Ile Ser Gly Ile Lys
65 70 75 80
Glu Phe Tyr Ile Lys Tyr Val Val Ser Gly Lys Thr Tyr Tyr Asp Asn
85 90 95
Asn Gly Thr Lys Asn Tyr Gln Val Ser Thr Thr Ser Ser Thr Thr Thr
100 105 110
Ala Ser Thr Thr Thr Ala Thr Arg Thr Thr Thr Ala Gly Thr Thr Ser
115 120 125
Thr Ser Thr Thr Ala Ala Pro Thr Ser Thr Ser Ser Gly Ser Phe Pro
130 135 140
Ser Gly Asn Ser Thr Val Ser Ser Trp Ile Lys Arg Gln Glu Lys Ile
145 150 155 160
Ser Arg Phe Ala Met Leu Arg Asn Ile Asn Pro Pro Gly Ser Ala Ala
165 170 175
Gly Phe Ile Ala Ala Ser Leu Ser Thr Ser Gly Pro Asp Tyr Tyr Tyr
180 185 190
Ser Trp Thr Arg Asp Ser Ala Leu Thr Ser Asn Leu Ile Ala Tyr Glu
195 200 205
Tyr Asn Thr Thr Leu Ser Gly Asn Thr Thr Ile Leu Asn Ile Leu Lys
210 215 220
Asp Tyr Val Lys Phe Ser Ile Ser Ser Gln Thr Thr Ser Thr Val Cys
225 230 235 240
Asn Cys Leu Gly Glu Pro Lys Phe Asn His Asp Gly Ser Ser Tyr Thr
245 250 255
Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Asn
260 265 270
Thr Phe Ile Leu Phe Ala Asp Ser Tyr Leu Asp Gln Thr Lys Asp Ala
275 280 285
Ser Tyr Val Thr Gly Thr Leu Lys Pro Ala Ile Phe Lys Asp Leu Asp
290 295 300
Tyr Val Val Asn Val Trp Ser Asn Gly Cys Tyr Asp Leu Trp Glu Glu
305 310 315 320
Val Asn Gly Val His Phe Tyr Thr Leu Met Val Met Arg Lys Gly Leu
325 330 335
Leu Leu Gly Ala Asp Phe Ala Lys Arg Asn Gly Asp Ser Thr Arg Ala
340 345 350
Ser Thr Tyr Thr Asn Thr Ala Ser Thr Ile Ala Thr Lys Ile Ser Ser
355 360 365
Phe Trp Val Ser Ser Ser Asn Trp Ile Gln Val Ser Gln Ser Val Thr
370 375 380
Ala Gly Val Ser Lys Lys Gly Leu Asp Val Ser Thr Leu Leu Ala Ala
385 390 395 400
Asn Leu Gly Ser Val Glu Asp Gly Phe Phe Thr Pro Gly Ser Glu Lys
405 410 415
Ile Leu Ala Thr Ala Val Ala Ile Glu Asp Ala Phe Ala Ser Leu Tyr
420 425 430
Pro Ile Asn Ser Asn Leu Pro Ser Tyr Leu Gly Asn Ser Ile Gly Arg
435 440 445
Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro
450 455 460
Trp Phe Leu Ala Val Asn Gly Phe Ala Glu Leu Tyr Tyr Arg Ala Ile
465 470 475 480
Lys Glu Trp Val Asn Asn Gly Ser Val Thr Val Ser Asn Ile Ser Leu
485 490 495
Ser Phe Phe Lys Lys Phe Asp Ser Ser Ala Thr Ala Gly Lys Thr Tyr
500 505 510
Thr Ala Gly Thr Ala Asp Phe Asn Asn Leu Ala Gln Asn Ile Ala Leu
515 520 525
Gly Ala Asp Arg Phe Leu Ser Thr Val Gln Thr His Ala Phe Asn Asn
530 535 540
Gly Ser Leu Ala Glu Glu Tyr Asp Arg Thr Thr Gly Val Ser Thr Gly
545 550 555 560
Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu Ile Thr Ala Ala Tyr
565 570 575
Ala Lys Ala Gly Ser Pro Ala Ala
580
<210> 67
<211> 584
<212> PRT
<213> 卷枝毛霉（Mucor circinelloides）
<400> 67
Glu Thr Val Pro Thr Thr Ala Ala Val Lys Val Lys Ser Phe Thr Tyr
1 5 10 15
Asp Gly Ser Thr Phe Ala Gly Gln Ile Tyr Val Lys Asn Ile Ala Tyr
20 25 30
Thr Lys Thr Val Thr Val Ile Tyr Ser Asp Ala Ser Asn Asn Trp Asn
35 40 45
Asn Asn Gly Asn Thr Ile Ala Ala Ser Tyr Ser Glu Gly Ile Ser Gly
50 55 60
Thr Asn Tyr Glu Tyr Trp Thr Phe Ser Ala Pro Val Ser Gly Ile Lys
65 70 75 80
Gln Phe Tyr Val Lys Tyr Val Val Ser Gly Thr Thr Tyr Tyr Asp Asn
85 90 95
Asn Asn Ser Gly Asn Tyr Gln Val Thr Thr Thr Thr Thr Thr Thr Ala
100 105 110
Pro Thr Ser Thr Thr Ser Gly Gly Ser Ser Thr Thr Thr Gly Gly Ser
115 120 125
Thr Thr Thr Ala Thr Ser Val Pro Thr Gly Val Pro Ser Gly Phe Pro
130 135 140
Thr Gly Asn Ser Ser Ile Ser Ser Trp Ile Asp Gly Gln Thr Ser Val
145 150 155 160
Ser Arg Tyr Ala Met Leu Arg Asn Ile Asn Pro Ala Gly Ala Val Ser
165 170 175
Gly Phe Ile Ala Ala Ser Met Ser Thr Ser Gly Pro Asp Tyr Phe Tyr
180 185 190
Ala Trp Thr Arg Asp Ser Ala Leu Thr Ser His Val Val Ala Tyr Asp
195 200 205
Tyr Asn Thr Thr Leu Ala Gly Asn Ser Thr Ile Leu Gly Leu Leu Lys
210 215 220
Asn Tyr Val Thr Phe Ser Ile Asn Ser Gln Thr Thr Ser Thr Val Cys
225 230 235 240
Asn Cys Leu Gly Glu Pro Lys Phe Asn Lys Asp Gly Ser Ser Tyr Thr
245 250 255
Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Ser Arg Ala Asp
260 265 270
Thr Phe Ile Leu Ile Ala Asp Ser Ile Leu Lys Gln Thr Gly Asp Ala
275 280 285
Thr Tyr Val Thr Gly Thr Leu Ala Pro Ala Ile Tyr Lys Asp Leu Asp
290 295 300
Tyr Val Val Ser Thr Trp Ser Asn Gly Cys Phe Asp Leu Trp Glu Glu
305 310 315 320
Val Asn Gly Val His Phe Tyr Thr Leu Met Val Met Arg Arg Gly Leu
325 330 335
Ile Lys Gly Ala Asn Phe Ala Ser Arg Asn Gly Asp Asn Thr Arg Ala
340 345 350
Asn Thr Tyr Thr Asn Thr Ala Ala Ser Ile Lys Thr Lys Ile Asp Ser
355 360 365
Phe Trp Asn Ser Asn Gly Asn Tyr Val Thr Val Ser Gln Ser Val Thr
370 375 380
Gly Gly Val Ser Lys Ala Gly Tyr Asp Ala Ser Val Leu Ile Ala Ala
385 390 395 400
Asn Leu Gly Ser Val Gln Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys
405 410 415
Met Leu Ala Thr Ala Val Ala Ile Glu Ser Lys Phe Ala Ser Leu Tyr
420 425 430
Ser Ile Asn Gln Asn Leu Asn Gly Tyr Leu Gly Asn Ala Ile Gly Arg
435 440 445
Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro
450 455 460
Trp Phe Ile Cys Thr Asn Ala Phe Ala Glu Leu Tyr Tyr Arg Ala Ile
465 470 475 480
Lys Glu Trp Phe Asn Asn Gly Gly Val Thr Val Thr Ser Ile Ser Leu
485 490 495
Asn Phe Phe Lys Lys Phe Asp Ser Ser Ala Ala Val Gly Thr Lys Tyr
500 505 510
Thr Val Gly Thr Ser Ala Phe Asn Ser Leu Val Gln Asn Val Ala Val
515 520 525
Ala Ala Asp Ala Phe Phe Ser Thr Val Lys Phe His Ala Ala Thr Asn
530 535 540
Gly Ser Met Ser Glu Gln Tyr Gly Arg Ser Asp Gly Leu Met Thr Gly
545 550 555 560
Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu Ile Ser Ala Ser Tyr
565 570 575
Ala Lys Ala Gly Ser Pro Ala Ala
580
<210> 68
<211> 581
<212> PRT
<213> Dichotomocladium elegans
<400> 68
Ala Thr Val Pro Lys Thr Gln Val Lys Leu Glu Ala Tyr Thr Tyr Ser
1 5 10 15
Asp Ser Ile Phe Ser Gly Arg Ile Phe Val Gln Asn Ile Asp Tyr Thr
20 25 30
Lys Ile Val Thr Val Tyr Trp Ser Asp Ala Ser Asn Lys Trp Asp Ser
35 40 45
Ser Lys Tyr Tyr Thr Glu Ala Ala Tyr Thr His Ser Ile Pro Gly Thr
50 55 60
Asn Tyr Glu Tyr Trp Asp Phe Ser Ala Thr Ile Gly Pro Ser Gly Ile
65 70 75 80
Lys Gln Phe Tyr Ile Ser Tyr Gln Val Arg Gly Val Thr Tyr Tyr Asp
85 90 95
Asn Asn Gly Gly Tyr Gly Val Asn Tyr Asp Val Ile Ser Ser Pro Pro
100 105 110
Thr Thr Ser Asp Ala Thr Thr Ile Pro Thr Thr Thr Thr Ser Ala Ile
115 120 125
Pro Ser Ser Thr Thr Thr Ile Pro Ala Ser Thr Gly Val Pro Ser Gly
130 135 140
Asn Ser Thr Ile Thr Val Trp Ala Asp Ser Gln His Lys Ile Ser Trp
145 150 155 160
Lys Ala Met Leu Ala Asn Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe
165 170 175
Ile Ala Ala Ser Leu Ser Thr Ser Gly Pro Asp Tyr Tyr Tyr Ala Trp
180 185 190
Thr Arg Asp Ala Ala Met Val Ala His Val Ile Val Asn Ala Tyr Asn
195 200 205
Thr Thr Lys Ala Gly Asp Ala Thr Thr Leu Gly Val Leu Lys Asp Phe
210 215 220
Val Thr Phe Gln Ile Lys Ala Met Ala Thr Ser Thr Val Cys Asn Cys
225 230 235 240
Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser Tyr Thr Gly Pro
245 250 255
Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe
260 265 270
Ile Leu Phe Ala Asp Ser Tyr Leu Ser Gln Thr Gly Asp Ala Ala Tyr
275 280 285
Val Thr Thr Leu Lys Arg Ala Ile Phe Thr Asp Leu Asp Tyr Val Val
290 295 300
Thr Thr Trp Gln Asp Asn Cys Phe Asp Leu Trp Glu Glu Val Asn Gly
305 310 315 320
Leu His Met Tyr Thr Leu Ala Val Met Arg Arg Ser Leu Val Asp Gly
325 330 335
Ala Ser Phe Ala Ala Arg Asn Gly Asp Asn Thr Arg Ala Ser Thr Tyr
340 345 350
Thr Asn Thr Ala Lys Ser Ile Glu Thr Lys Leu Ala Ser Phe Tyr Asn
355 360 365
Ser Ser Ser Asn Tyr Val Val Val Thr Lys Asn Phe Ala Gly Gly Val
370 375 380
Asn Lys Ala Gly Leu Asp Thr Ser Thr Leu Ile Ala Ala Asn Thr Ala
385 390 395 400
Gly Leu Gly Asp Gly Phe Phe Thr Pro Gly Ser Pro Glu Ile Leu Ala
405 410 415
Thr Ala Ala Ala Ile Glu Lys Ser Phe Ala Asp Leu Tyr Gly Ile Asn
420 425 430
Lys Gln Ile Pro Asn Trp Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu
435 440 445
Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile
450 455 460
Cys Thr Ala Thr Phe Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp
465 470 475 480
Gln His Ala Gly Ser Val Thr Val Asn Asn Val Asn Leu Pro Phe Phe
485 490 495
Lys Lys Phe Asp Ser Ser Thr Ser Ser Gly Thr Thr Tyr Thr Val Gly
500 505 510
Thr Ser Ser Phe Asp Ser Leu Ile Ser Lys Val Ala Tyr Ala Ala Asp
515 520 525
Asn Phe Phe Ser Thr Ile Lys Tyr His Ala Ala Thr Asn Gly Ser Met
530 535 540
Ser Glu Gln Phe Asn Arg Asp Thr Gly Phe Met Thr Gly Ala Arg Asp
545 550 555 560
Leu Thr Trp Ser His Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys Ala
565 570 575
Gly Thr Pro Val Tyr
580
<210> 69
<211> 582
<212> PRT
<213> Fennellomyces属物种
<400> 69
Gln Thr Val Pro Ser Val Pro Ile Lys Leu Glu Ser Tyr Thr Tyr Ser
1 5 10 15
Asp Asn Val Phe Ala Gly Arg Ile Phe Ile Arg Asn Ile Ala Tyr Thr
20 25 30
Lys Val Val Lys Val Phe Trp Ser Asp Glu Ser Gly Asn Trp Asn Gly
35 40 45
Asn Gly Asn Tyr Val Asp Ala Ser Tyr Ser Gly Ser Ile Ser Gly Thr
50 55 60
Asn Tyr Glu Tyr Trp Glu Phe Ser Thr Thr Ile Gly Ser Ala Gly Ile
65 70 75 80
Ser Gln Ser Tyr Leu Arg Tyr Asp Val Ser Gly Ser Thr Tyr Tyr Asp
85 90 95
Asn Asn Gly Asn Ala Asn Tyr Asp Ile Glu Glu Asp Ser Thr Pro Thr
100 105 110
Pro Thr Thr Thr Thr Thr Ser Thr Thr Thr Ile Thr Thr Thr Thr Thr
115 120 125
Ser Gly Thr Ser Thr Pro Thr Ser Val Pro Thr Glu Val Pro Ala Gly
130 135 140
Asn Thr Thr Val Thr Asp Trp Val Asn Asp Gln Leu Glu Ile Ser Trp
145 150 155 160
Pro Ser Leu Leu Lys Asn Val Asn Pro Ser Gly Ala Val Thr Gly Phe
165 170 175
Ile Ala Ala Ser Leu Ser Thr Asn Asp Pro Asp Tyr Phe Tyr Cys Trp
180 185 190
Thr Arg Asp Ala Ala Leu Val Ala Arg Val Met Val Tyr Met Tyr Asn
195 200 205
Thr Thr Glu Ala Gly Asp Thr Ser Leu Arg Ser Lys Leu Gln Asp Tyr
210 215 220
Val Thr Phe Gln Ile Asn Ser Met Lys Thr Ala Thr Val Cys Asn Cys
225 230 235 240
Leu Gly Glu Pro Lys Phe Asn Lys Asp Gly Ser Gly Tyr Ser Gly Ala
245 250 255
Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Asp Arg Ala Ile Thr Leu
260 265 270
Ile Leu Phe Ala Asp Ser Phe Ile Ala Gln Gly Gly Asp Val Ser Tyr
275 280 285
Ile Thr Asn Thr Leu Lys Pro Ala Ile Tyr Thr Asn Leu Asp Tyr Val
290 295 300
Val Asn Thr Trp Ser Asn Val Cys Phe Asp Leu Trp Glu Glu Val Asn
305 310 315 320
Gly Val His Ile Tyr Thr Leu Ser Val Met Arg Lys Gly Leu Leu Glu
325 330 335
Gly Ala Asp Phe Ala Ser Arg Asn Gly Asp Ser Thr Arg Ala Asn Thr
340 345 350
Tyr Arg Ser Thr Ala Ser Ser Ile Lys Thr Arg Leu Glu Ser Phe Trp
355 360 365
Ser Ser Ser Asn Asn Tyr Ile Thr Val Thr Gln Ser Tyr Ser Gly Gly
370 375 380
Val Asn Lys Ala Gly Leu Asp Val Ser Thr Leu Leu Ala Ala Asn Gly
385 390 395 400
Ala Ser Met Asn Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu
405 410 415
Ala Thr Ala Val Ala Ile Glu Asn Ser Phe Ala Gly Ile Tyr Ala Val
420 425 430
Asn Gln Asn Arg Pro Asp Trp Lys Gly Thr Ala Ile Gly Arg Tyr Pro
435 440 445
Glu Asp Thr Tyr Asp Gly His Gly Asn Ser Gln Gly Asn Pro Trp Phe
450 455 460
Ile Ala Thr Ala Ala Tyr Ala Glu Met Tyr Tyr Arg Ala Ile Leu Glu
465 470 475 480
Trp Gln Gln Gln Pro Ser Ile Thr Val Asn Ser Ile Asn Leu Ser Phe
485 490 495
Phe Lys Lys Phe Asp Ser Ser Ala Ala Val Gly Thr Val Tyr Lys Pro
500 505 510
Gly Thr Gln Ala Phe Asn Asn Met Val Ser Asn Val Ala Phe Ala Ala
515 520 525
Asp Glu Phe Phe Ser Thr Met Asn Phe His Ser Ala Thr Asn Gly Ser
530 535 540
Met Ser Glu Gln Tyr Asn Arg Asn Thr Gly Ile Met Gln Gly Ala Arg
545 550 555 560
Asp Leu Thr Trp Ser His Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys
565 570 575
Leu Gly Thr Pro Val Phe
580
<210> 70
<211> 577
<212> PRT
<213> 桃吉尔霉（Gilbertella persicaria）
<400> 70
Glu Thr Val Pro Thr Ser Ala Gln Val Gln Val Lys Ser Phe Asn Tyr
1 5 10 15
Asp Gly Ser Thr Leu Ser Gly Gln Ile Tyr Val Gln Asn Ile Ala Tyr
20 25 30
Glu Lys Thr Val Thr Val Val Tyr Ser Asp Gly Ser Asp Asn Trp Asn
35 40 45
Asn Asn Gly Asn Thr Ile Ala Ala Ser Tyr Ser Ser Ser Ile Ser Gly
50 55 60
Ser Asn Tyr Glu Tyr Trp Thr Phe Ser Ser Ser Val Pro Ser Ile Lys
65 70 75 80
Gln Phe Tyr Ile Lys Tyr Val Val Ala Gly Lys Thr Tyr Tyr Asp Asn
85 90 95
Asn Gly Thr Lys Asn Tyr Gln Val Ser Ala Ser Thr Pro Thr Thr Thr
100 105 110
Thr Thr Thr Thr Ser Ser Gly Ala Thr Lys Thr Thr Thr Thr Ile Gly
115 120 125
Pro Thr Ser Thr Ser Thr Val Phe Pro Ser Gly Asn Ser Thr Ile Ser
130 135 140
Ser Trp Leu Lys Gly Gln Ile Glu Thr Ser Arg Phe Ala Met Leu Arg
145 150 155 160
Asn Ile Asn Pro Ala Gly Thr Val Lys Gly Phe Ile Ala Ala Ser Leu
165 170 175
Ser Thr Ala Asn Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ala Ala
180 185 190
Leu Val Gly His Val Ile Ala Asn Asp Tyr Asn Arg Thr Leu Ala Gly
195 200 205
Asn Ser Thr Tyr Leu Gly Leu Leu Lys Asp Tyr Val Thr Phe Ser Val
210 215 220
Asn Ser Gln Ser Thr Ser Thr Ile Cys Asn Cys Leu Gly Glu Pro Lys
225 230 235 240
Phe Asn Lys Asp Gly Ser Gly Tyr Ser Gly Ala Trp Gly Arg Pro Gln
245 250 255
Asn Asp Gly Pro Ala Glu Arg Ala Asp Thr Phe Ile Leu Ile Ala Asp
260 265 270
Ser Ile Leu Lys Gln Thr Gly Asp Ala Thr Tyr Val Thr Gly Thr Leu
275 280 285
Arg Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Val Trp Ser
290 295 300
Asn Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr
305 310 315 320
Thr Leu Met Val Met Arg Arg Ser Leu Ile Leu Gly Ala Asn Phe Ala
325 330 335
Ser Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Thr Asn Thr Ala
340 345 350
Asn Ser Ile Lys Thr Lys Ile Asp Thr Phe Trp Ser Ser Ser Asn Asn
355 360 365
Tyr Val Ala Val Ser Gln Ser Val Thr Gly Gly Val Asn Lys Ala Gly
370 375 380
Tyr Asp Val Ala Asn Leu Ile Ala Ala Asn Val Gly Ser Leu Asp Asp
385 390 395 400
Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala Thr Ala Val Ala
405 410 415
Val Glu Ser Lys Phe Ala Ser Ile Tyr Gly Val Asn Gln Asn Leu Pro
420 425 430
Ser Trp Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn
435 440 445
Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Asn Thr
450 455 460
Tyr Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Thr Asn Asn Gly
465 470 475 480
Gly Val Thr Val Thr Asn Val Asn Phe Asn Phe Phe Lys Lys Phe Asp
485 490 495
Ser Ser Ala Ser Val Gly Thr Lys Tyr Thr Val Gly Thr Ser Ala Phe
500 505 510
Asn Thr Leu Thr Gln Asn Val Ala Leu Ala Ala Asp Asn Phe Phe Ser
515 520 525
Thr Val Lys Val His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Phe
530 535 540
Gly Arg Asp Ser Gly Val Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
545 550 555 560
His Ala Ser Leu Ile Thr Ala Ala Leu Ala Lys Thr Gly Ala Pro Val
565 570 575
Ala
<210> 71
<211> 585
<212> PRT
<213> 卷枝毛霉（Mucor circinelloides）
<400> 71
Glu Thr Val Pro Thr Thr Glu Ala Val Lys Val Lys Ser Phe Thr Tyr
1 5 10 15
Asp Gly Ser Thr Leu Ala Gly Gln Ile Tyr Ile Lys Asn Ile Ala Tyr
20 25 30
Thr Lys Thr Val Thr Val Ile Tyr Ser Asp Ala Ser Asp Asn Trp Asn
35 40 45
Asn Asn Gly Asn Thr Ile Ala Ala Ser Tyr Ser Ala Ala Ile Ala Gly
50 55 60
Thr Asn Tyr Glu Tyr Trp Thr Phe Ser Ala Pro Val Ser Gly Ile Lys
65 70 75 80
Gln Phe Tyr Val Lys Tyr Val Val Ser Gly Thr Thr Tyr Tyr Asp Asn
85 90 95
Asn Asn Ser Gly Asn Tyr Gln Val Ser Val Thr Thr Thr Thr Thr Thr
100 105 110
Ala Pro Thr Thr Thr Thr Ser Gly Gly Ser Ser Thr Thr Thr Gly Gly
115 120 125
Ser Thr Thr Thr Ala Thr Ser Val Pro Thr Gly Val Pro Ser Gly Phe
130 135 140
Pro Thr Gly Asn Ser Thr Ile Ser Ser Trp Ile Asp Gly Gln Thr Ser
145 150 155 160
Val Ser Arg Tyr Ala Met Leu Arg Asn Ile Asn Pro Ala Gly Ala Val
165 170 175
Thr Gly Phe Ile Ala Ala Ser Met Ser Thr Ser Gly Pro Asp Tyr Phe
180 185 190
Tyr Ala Trp Thr Arg Asp Ser Ala Leu Thr Ser His Val Val Ala Tyr
195 200 205
Asp Tyr Asn Thr Thr Leu Ala Gly Asn Ser Thr Ile Leu Gly Leu Leu
210 215 220
Lys Asn Tyr Val Thr Phe Ser Leu Asn Ser Gln Thr Thr Ser Thr Val
225 230 235 240
Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Lys Asp Gly Ser Gly Tyr
245 250 255
Ser Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Ser Arg Ala
260 265 270
Asp Thr Phe Ile Leu Ile Ala Asp Ser Ile Leu Lys Gln Thr Gly Asp
275 280 285
Ala Thr Tyr Val Thr Gly Thr Leu Ala Pro Ala Ile Tyr Lys Asp Leu
290 295 300
Asp Tyr Val Val Ser Thr Trp Ser Asn Gly Cys Phe Asp Leu Trp Glu
305 310 315 320
Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Val Met Arg Arg Gly
325 330 335
Leu Val Lys Gly Ala Asn Phe Ala Ser Arg Asn Gly Asp Ser Thr Arg
340 345 350
Ala Thr Thr Tyr Thr Asn Thr Ala Ala Ser Ile Lys Thr Lys Ile Asp
355 360 365
Ser Phe Trp Asn Ser Asn Gly Gln Tyr Val Ser Val Ser Gln Ser Val
370 375 380
Thr Gly Gly Val Ser Lys Ala Gly Tyr Asp Ala Ser Val Leu Ile Ala
385 390 395 400
Ser Asn Leu Gly Ser Leu Gln Asp Gly Phe Tyr Thr Pro Gly Ser Asp
405 410 415
Lys Met Leu Ala Thr Ala Val Ala Ile Glu Ser Lys Phe Ala Ser Leu
420 425 430
Tyr Ser Ile Asn Gln Asn Leu Asn Gly Tyr Leu Gly Asn Ala Ile Gly
435 440 445
Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn
450 455 460
Pro Trp Phe Ile Cys Thr Asn Ala Phe Ala Glu Leu Tyr Tyr Arg Ala
465 470 475 480
Ile Lys Glu Trp Phe Asn Asn Gly Gly Val Thr Val Thr Ser Ile Ser
485 490 495
Leu Asn Phe Phe Lys Lys Phe Asp Ser Ser Ala Ala Val Gly Thr Lys
500 505 510
Tyr Thr Val Gly Thr Ser Ser Phe Asn Ser Leu Val Gln Asn Val Ala
515 520 525
Val Ala Ala Asp Ala Phe Phe Ser Thr Ile Lys Phe His Ala Ala Thr
530 535 540
Asn Gly Ser Met Ser Glu Gln Tyr Gly Arg Thr Asp Gly Leu Met Thr
545 550 555 560
Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu Ile Ser Ala Ser
565 570 575
Tyr Ala Lys Ala Gly Ser Pro Ala Ala
580 585
<210> 72
<211> 583
<212> PRT
<213> 伞形卷霉（Circinella umbellata）
<400> 72
Ala Pro Thr Ser Glu Ile Glu Leu Asp Ser Tyr Thr Tyr Thr Gly Gly
1 5 10 15
Val Phe Ser Gly Arg Leu Tyr Val Lys Asn Ile Ala Tyr Thr Lys Glu
20 25 30
Val Asn Val Tyr Trp Ser Asp Ala Ser Glu Asp Trp Ala Asn Asn Gly
35 40 45
Asn Tyr Val Ala Ala Thr Tyr Ser Glu Ala Ile Ser Gly Thr Asn Tyr
50 55 60
Glu Tyr Trp Glu Phe Ser Ala Lys Ile Gly Ser Ser Gly Ile Ser Glu
65 70 75 80
Ser Tyr Ile Lys Tyr Thr Val Ser Gly Ser Thr Tyr Tyr Asp Asn Asn
85 90 95
Gly Gly Lys Asn Tyr Ala Ile Thr Glu Thr Thr Thr Pro Thr Ile Thr
100 105 110
Ala Thr Pro Thr Ser Thr Thr Ala Thr Thr Thr Ile Ser Ser Thr Thr
115 120 125
Thr Thr Ala Thr Ser Ile Pro Thr Ser Val Pro Ser Thr Val Pro Glu
130 135 140
Gly Asn Val Thr Val Thr Glu Trp Val Asn Lys Gln Leu Lys Ile Ser
145 150 155 160
Trp Ser Asp Leu Leu Gln Asn Val Asn Pro Ser Gly Thr Val Thr Gly
165 170 175
Phe Ile Ala Ala Ser Leu Ser Thr Ser Asn Pro Asp Tyr Phe Tyr Cys
180 185 190
Trp Thr Arg Asp Ala Ala Met Val Ala Arg Val Met Thr Tyr Met Tyr
195 200 205
Asn Thr Thr Glu Ala Gly Asp Ser Ser Leu Glu Ser Ala Leu Lys Asp
210 215 220
Tyr Ile Thr Phe Gln Ile Asn Ser Met Lys Thr Ala Thr Ala Cys Asn
225 230 235 240
Cys Leu Gly Glu Pro Lys Phe Asn Thr Asp Gly Ser Gly Tyr Ser Gly
245 250 255
Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Thr Thr
260 265 270
Met Ile Leu Phe Ala Asp Ser Tyr Leu Ala Gln Gly Gly Asp Thr Ser
275 280 285
Tyr Val Thr Asn Thr Leu Lys Pro Ala Ile Tyr Thr Asn Leu Asp Tyr
290 295 300
Val Val Gly Thr Trp Ser Asn Asn Cys Phe Asp Leu Trp Glu Glu Val
305 310 315 320
Asn Gly Val His Ile Phe Thr Leu Ala Val Met Arg Lys Ser Leu Leu
325 330 335
Asp Gly Ala Asp Phe Ala Ala Arg Asn Gly Asp Thr Ser Arg Val Ser
340 345 350
Gly Tyr Gln Ser Thr Ala Ser Ser Ile Lys Thr Lys Leu Glu Ser Phe
355 360 365
Trp Ser Ser Ser Asn Asn Tyr Ile Thr Val Thr Gln Ser Tyr Ser Gly
370 375 380
Gly Val Gln Lys Ala Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn
385 390 395 400
Gln Gly Ser Met Gly Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys Met
405 410 415
Leu Ala Thr Ala Val Ala Ile Glu Asn Ser Phe Ala Asn Ile Tyr Thr
420 425 430
Ile Asn Lys Asn Lys Pro Ser Trp Leu Gly Thr Ala Ile Gly Arg Tyr
435 440 445
Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Gly Gln Gly Asn Pro Trp
450 455 460
Phe Ile Ala Thr Ala Thr Tyr Ala Glu Leu Tyr Tyr Arg Ala Ile Leu
465 470 475 480
Glu Trp Gln Gln Gln Glu Ser Val Thr Val Asn Ser Val Asn Phe Asp
485 490 495
Phe Phe Ser Lys Phe Asp Ser Ser Ala Lys Val Gly Thr Val Tyr Thr
500 505 510
Pro Gly Thr Asp Thr Phe Asn Thr Met Val Ser Asn Val Ala Phe Ala
515 520 525
Ala Asp Glu Phe Leu Ser Thr Met Glu His Tyr Ala Ala Thr Asn Gly
530 535 540
Ser Met Ser Glu Gln Phe Asn Arg Glu Thr Gly Ser Leu Thr Gly Ala
545 550 555 560
Arg Asp Leu Thr Trp Ser His Ala Ala Phe Ile Thr Ala Gly Lys Ala
565 570 575
Lys Leu Gly Ile Pro Ser Phe
580
<210> 73
<211> 585
<212> PRT
<213> Mucor cordense
<400> 73
Glu Thr Val Pro Thr Thr Ala Asn Val Leu Val Lys Ser Tyr Thr Trp
1 5 10 15
Asp Gly Ala Thr Leu Ser Gly Gln Ile Tyr Ile Lys Asn Leu Ala Tyr
20 25 30
Ala Lys Val Val Ser Val Ile Tyr Ser Asp Ala Asn Asp Asn Trp Asn
35 40 45
Asn Asn Gly Asn Lys Val Ala Ala Ser Tyr Ser Ala Gly Ile Asp Gly
50 55 60
Thr Asn Tyr Glu Tyr Trp Thr Phe Ser Gly Ala Val Ser Gly Ile Lys
65 70 75 80
Gln Phe Tyr Val Lys Tyr Asp Val Ser Gly Thr Ser Tyr Tyr Asp Asn
85 90 95
Asn Gly Thr Lys Asn Tyr Gln Val Thr Lys Thr Ser Thr Thr Thr Thr
100 105 110
Ala Ala Thr Thr Thr Thr Thr Ala Thr Thr Thr Thr Gly Gly Thr Gly
115 120 125
Thr Thr Thr Thr Thr Thr Ala Thr Ala Thr Ala Thr Ser Thr Asp Phe
130 135 140
Pro Thr Gly Asn Ser Thr Ile Thr Thr Trp Leu Lys Ser Gln Glu Asp
145 150 155 160
Ile Ser Arg Gly Ala Met Leu Arg Asn Ile Asn Pro Pro Gly Ala Ala
165 170 175
Thr Gly Phe Ile Ala Ala Ser Leu Ser Thr Ser Gly Pro Asp Tyr Tyr
180 185 190
Tyr Ala Trp Thr Arg Asp Ser Ala Leu Thr Ser His Val Ile Ala His
195 200 205
Asp Tyr Asn Thr Thr Leu Ala Gly Asn Thr Thr Ile Leu Asn Ile Leu
210 215 220
Lys Asp Tyr Val Thr Phe Ser Val Lys Ser Gln Ser Val Ser Thr Val
225 230 235 240
Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser Tyr
245 250 255
Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala
260 265 270
Ser Thr Phe Ile Leu Phe Gly Asp Ser Tyr Leu Lys Gln Thr Gly Asp
275 280 285
Ala Thr Tyr Val Thr Gly Thr Leu Ala Pro Ala Ile Tyr Lys Asp Leu
290 295 300
Asp Tyr Val Val Asn Thr Trp Ser Asn Gly Cys Phe Asp Leu Trp Glu
305 310 315 320
Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Ser Met Arg Arg Gly
325 330 335
Leu Leu Asp Gly Ala Asn Phe Ala Lys Arg Asn Gly Asp Thr Thr Arg
340 345 350
Ala Thr Thr Tyr Thr Asn Thr Ala Ala Ser Ile Ala Thr Lys Ile Asp
355 360 365
Thr Phe Trp Val Ser Ser Gly Asn Tyr Val Gln Val Ser Gln Ser Val
370 375 380
Thr Gly Gly Val Ser Lys Ala Gly Tyr Asp Cys Ala Asn Ile Leu Ala
385 390 395 400
Ala Asn Met Ala Ala Asn His Asp Gly Phe Tyr Thr Pro Gly Ser Ser
405 410 415
Lys Ile Leu Ala Thr Ala Val Ala Ile Glu Ser Lys Phe Ala Ser Leu
420 425 430
Tyr Ser Ile Asn Ser Gly Leu Ala Ser Trp Leu Gly Thr Ala Ile Gly
435 440 445
Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn
450 455 460
Pro Trp Phe Ile Cys Thr Asn Ala Phe Ala Glu Leu Tyr Tyr Arg Ala
465 470 475 480
Ile Lys Glu Trp Thr Gln Ala Gly Ser Val Thr Val Asp Ser Thr Ser
485 490 495
Leu Asn Phe Phe Lys Lys Phe Asp Ser Ser Ala Ala Ala Gly Thr Lys
500 505 510
Tyr Thr Val Gly Thr Ser Ala Phe Thr Asn Leu Val Gln Asn Ile Ala
515 520 525
Asn Gly Ala Asp Lys Phe Leu Ser Thr Ser Lys Phe His Ala Ala Thr
530 535 540
Asn Gly Ser Met Ser Glu Gln Tyr Asn Arg Asp Ser Gly Leu Met Thr
545 550 555 560
Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu Ile Ser Ala Ser
565 570 575
Arg Ala Lys Ala Gly Ser Pro Ser Leu
580 585
<210> 74
<211> 590
<212> PRT
<213> Phascolomyces articulosus
<400> 74
Asp Val Pro Thr Ser Ala Asn Ile Glu Leu Asp Tyr Tyr Thr Tyr Lys
1 5 10 15
Asp Lys Val Phe Ser Gly Arg Ile Tyr Val Lys Asn Ile Ala Tyr Glu
20 25 30
Lys Thr Val Ala Val Tyr Trp Ser Asp Ala Ser Gly Asp Trp Asn Asn
35 40 45
Asn Gly Asn Asn Val Ala Ala Ser Tyr Ser Glu Ser Ile Ser Gly Thr
50 55 60
Asp Tyr Glu Tyr Trp Asp Phe Ser Thr Thr Ile Gly Ser Gly Gly Ile
65 70 75 80
Lys Gln Ser Tyr Leu Lys Tyr Thr Val Ser Gly Asn Thr Tyr Tyr Asp
85 90 95
Asn Asn Gly Ser Asn Asn Tyr Asp Ile Thr Glu Thr Thr Ala Thr Thr
100 105 110
Thr Thr Thr Thr Thr Thr Thr Thr Thr Thr Gly Ser Thr Ala Thr Thr
115 120 125
Ser Thr Thr Ser Gly Pro Thr Ser Thr Thr Ser Thr Thr Pro Gly Pro
130 135 140
Thr Pro Thr Asn Val Pro Asp Gly Asn Val Thr Val Thr Glu Trp Val
145 150 155 160
Asn Thr Gln Phe Ala Ile Ser Trp Pro Thr Leu Leu Lys Asn Val Asn
165 170 175
Pro Ser Gly Thr Val Lys Gly Phe Ile Ala Ala Ser Leu Ser Thr Asn
180 185 190
Asn Pro Asp Tyr Phe Tyr Ser Trp Thr Arg Asp Ser Ala Leu Val Ala
195 200 205
His Thr Met Thr Tyr Leu Tyr Asn Thr Ser Glu Ala Gly Asp Ser Thr
210 215 220
Ile Glu Ser Ala Leu Lys Asp Tyr Val Thr Phe Ser Ile Asn Ala Met
225 230 235 240
Asn Ala Ala Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Thr
245 250 255
Asp Gly Ser Gly Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly
260 265 270
Pro Ala Ser Arg Ala Thr Thr Met Ile Leu Phe Ala Asp Ser Phe Leu
275 280 285
Ala Gln Gly Gly Asp Val Ser Tyr Val Ile Asn Thr Leu Lys Pro Ala
290 295 300
Ile Tyr Lys Asp Leu Asp Tyr Val Val Ser Thr Trp Ser Asn Thr Cys
305 310 315 320
Tyr Asp Leu Trp Glu Glu Val Asn Gly Val His Ile Tyr Thr Leu Ser
325 330 335
Val Met Arg Arg Ala Leu Ile Asp Gly Ala Asn Phe Ala Gln Arg Asn
340 345 350
Gly Asp Thr Ser Arg Val Ser Gly Tyr Thr Ser Thr Ala Thr Thr Ile
355 360 365
Lys Thr Arg Leu Glu Ser Phe Trp Ser Asp Ser Asn Asn Tyr Ile Thr
370 375 380
Val Thr Gln Ser Tyr Ser Gly Gly Val Gln Lys Ala Gly Leu Asp Val
385 390 395 400
Ser Thr Leu Ile Ala Ala Asn Ile Gly Ser Val Gly Asp Gly Phe Tyr
405 410 415
Thr Pro Gly Ser Asp Lys Val Leu Ala Thr Ala Val Ala Ile Glu Lys
420 425 430
Ser Phe Ala Asn Leu Tyr Thr Ile Asn Gln Asn Lys Pro Ser Trp Leu
435 440 445
Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asp Gly Tyr Gly
450 455 460
Asn Ser Lys Gly Asn Pro Trp Phe Ile Ala Thr Ala Thr Tyr Ala Glu
465 470 475 480
Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Gln Gln Ala Ser Val Thr
485 490 495
Val Asn Ser Ile Asn Leu Gly Phe Phe Ser Lys Phe Asp Ser Ser Ala
500 505 510
Ser Val Gly Thr Val Tyr Thr Pro Gly Thr Asp Ser Phe Ala Asn Met
515 520 525
Val Ser Asn Val Ala Phe Ala Ala Asp Glu Phe Leu Ser Thr Ile Asp
530 535 540
Tyr His Ala Met Asn Asn Gly Ser Met His Glu Gln Tyr Asn Arg Asp
545 550 555 560
Thr Gly Ile Ser Gln Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala
565 570 575
Phe Ile Thr Ala Ala Lys Ala Lys Leu Gly Ala Pro Ala Phe
580 585 590
<210> 75
<211> 578
<212> PRT
<213> 小孢根霉（Rhizopus microsporus）
<400> 75
Val Pro Thr Ser Ala Ala Val Gln Val Glu Ser Tyr Lys Tyr Asp Gly
1 5 10 15
Thr Thr Phe Ser Gly Arg Ile Phe Val Lys Asn Ile Ala Tyr Ser Lys
20 25 30
Val Val Thr Val Ile Tyr Ser Asp Gly Ser Asp Asn Trp Asn Asn Asn
35 40 45
Asn Asn Lys Ile Ser Ala Ala Tyr Ser Glu Ala Ile Ser Gly Ser Asn
50 55 60
Tyr Glu Tyr Trp Thr Phe Ser Ala Lys Leu Ser Gly Ile Lys Gln Phe
65 70 75 80
Tyr Val Lys Tyr Glu Val Ser Gly Ser Thr Tyr Tyr Asp Asn Asn Gly
85 90 95
Thr Lys Asn Tyr Gln Val Gln Ala Thr Ser Ala Thr Ser Thr Thr Ala
100 105 110
Thr Ala Thr Thr Thr Thr Ser Thr Ser Thr Thr Thr Thr Ser Thr Gly
115 120 125
Pro Thr Ser Thr Ala Ser Val Ser Phe Pro Thr Gly Asn Ser Thr Ile
130 135 140
Ser Ser Trp Ile Lys Asn Gln Glu Glu Ile Ser Arg Phe Ala Met Leu
145 150 155 160
Arg Asn Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser
165 170 175
Leu Ser Thr Ala Gly Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser
180 185 190
Ala Leu Thr Ala Asn Val Ile Ala Tyr Glu Tyr Asn Thr Thr Phe Ala
195 200 205
Gly Asn Thr Thr Leu Leu Lys Tyr Leu Lys Asp Tyr Val Thr Phe Ser
210 215 220
Val Lys Ser Gln Ser Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro
225 230 235 240
Lys Phe Asn Ala Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro
245 250 255
Gln Asn Asp Gly Pro Ala Glu Arg Ala Val Thr Phe Met Leu Ile Ala
260 265 270
Asp Ser Tyr Leu Thr Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr
275 280 285
Leu Lys Pro Ala Ile Phe Lys Asp Leu Asp Tyr Val Val Ser Val Trp
290 295 300
Ser Asn Gly Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Val His Phe
305 310 315 320
Tyr Thr Leu Met Val Met Arg Lys Gly Leu Ile Leu Gly Ala Asp Phe
325 330 335
Ala Ala Arg Asn Gly Asp Ser Ser Arg Ala Ser Thr Tyr Lys Asn Thr
340 345 350
Ala Ser Thr Met Glu Ser Lys Ile Ser Ser Phe Trp Ser Asp Ser Asn
355 360 365
Asn Tyr Val Gln Val Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys
370 375 380
Gly Leu Asp Val Ser Thr Leu Leu Ala Ala Asn Ile Gly Ser Leu Pro
385 390 395 400
Asp Gly Phe Phe Thr Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val
405 410 415
Ala Leu Glu Asn Ala Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu
420 425 430
Pro Ser Tyr Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
435 440 445
Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn
450 455 460
Ala Tyr Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Ile Ser Asn
465 470 475 480
Gly Lys Val Thr Val Ser Asn Ile Ser Leu Pro Phe Phe Lys Lys Phe
485 490 495
Asp Ser Ser Ala Thr Ser Gly Lys Thr Tyr Thr Ala Gly Thr Ser Asp
500 505 510
Phe Asn Asn Leu Ala Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu
515 520 525
Ser Thr Val Lys Phe His Ala Tyr Asn Asn Gly Ser Leu Ser Glu Glu
530 535 540
Tyr Asp Arg Ser Thr Gly Met Ser Thr Gly Ala Arg Asp Leu Thr Trp
545 550 555 560
Ser His Ala Ser Leu Ile Thr Ala Ala Tyr Ala Lys Ala Gly Ser Pro
565 570 575
Ala Ala
<210> 76
<211> 443
<212> PRT
<213> 伞菌霉（Spinellus fusiger）
<400> 76
Val Pro Ser Gly Asn Ser Thr Ile Thr Ala Trp Gly Ser Lys Gln Asp
1 5 10 15
Gly Ile Ser Phe Ser Thr Met Leu Gly Asn Ile Asn Pro Pro Gly Ser
20 25 30
Ser Lys Gly Phe Ile Ala Ala Ser Leu Ser Thr Ala Gly Pro Asn Tyr
35 40 45
Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu Val Ala Arg Ala Ile Thr
50 55 60
Tyr Lys Tyr Ser Thr Ser Tyr Gln Asn Asp Pro Lys Ile Leu Gly Leu
65 70 75 80
Leu Lys Asp Tyr Val Thr Tyr Gln Val Asn Glu Gln Thr Glu Ser Thr
85 90 95
Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser
100 105 110
Phe Ser Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg
115 120 125
Ala Ser Thr Met Ile Leu Phe Ala Lys Ser Tyr Tyr Ala Gln Thr Asn
130 135 140
Asp Val Gly Tyr Val Ser Asn Thr Leu Lys Pro Ala Ile Tyr Lys Asp
145 150 155 160
Leu Asp Tyr Ile Val Asn Val Trp Gly Asn Asn Cys Phe Asp Leu Trp
165 170 175
Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Met Met Arg His
180 185 190
Gly Leu Val Gln Gly Ser Ile Phe Ala Asn Thr Leu Gly Asp Ser Thr
195 200 205
Arg Ala Asn Thr Tyr Lys Thr Ala Ala Gln Asn Ile Lys Asn Arg Ile
210 215 220
Asp Thr Phe Trp Glu Ser Gly Ser Asn Tyr Ile Val Val Thr Gln Asn
225 230 235 240
Gln Ser Ala Gly Val Asn Lys Pro Ser Gly Leu Asp Val Ala Val Leu
245 250 255
Leu Ala Ala Asn Gln Gly Gly Leu Gly Asp Gly Val Tyr Thr Pro Gly
260 265 270
Ser Asp Lys Val Leu Ala Thr Ala Val Ala Leu Glu Lys Ser Phe Ala
275 280 285
Ser Leu Tyr Pro Ile Asn Lys Asn Leu Pro Ser Tyr Tyr Gly Thr Ala
290 295 300
Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn Ser Glu
305 310 315 320
Ala Asn Pro Trp Phe Ile Ala Thr Thr Thr Tyr Ala Glu Leu Tyr Tyr
325 330 335
Arg Ala Ile Ser Glu Trp Thr Asn Gly Ser Gly Val Thr Val Asn Ser
340 345 350
Ile Asn Lys Glu Phe Phe Ser Lys Phe Asp Ala Ser Ala Thr Asn Gly
355 360 365
Lys Val Tyr Thr Pro Gly Ser Asp Ser Phe Asn Ser Leu Val Asn Asn
370 375 380
Val Ala Ile Ala Ala Asp Asn Phe Leu Ser Thr Val Arg Tyr His Gln
385 390 395 400
Thr Ser Asn Gly Ser Leu Ser Glu Gln Phe Asn Arg Tyr Thr Gly Phe
405 410 415
Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala Met Val Thr
420 425 430
Ala Leu Ala Ala Lys Ala Gly Thr Pro Ser Pro
435 440
<210> 77
<211> 588
<212> PRT
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 77
Ala Thr Val Pro Thr Thr Gln Val Gln Leu Asp Tyr Tyr Thr Tyr Ser
1 5 10 15
Asn Asn Val Leu Ser Gly Arg Ile Tyr Val Gln Asn Ile Ala Tyr Ser
20 25 30
Lys Val Val Lys Val Ile Tyr Ser Asp Ala Ser Gly Asn Trp Asn Asn
35 40 45
Asn Gly Asn Thr Ile Ser Ala Ser Tyr Val Glu Ser Ile Ser Gly Thr
50 55 60
Asn Tyr Glu Tyr Trp Asp Phe Ser Ala Thr Ile Gly Thr Ala Gly Ile
65 70 75 80
Lys Gln Phe Tyr Leu Arg Tyr Asp Val Ser Gly Ser Thr Tyr Tyr Asp
85 90 95
Asn Asn Gly Gly Asp Asn Asn Asn Tyr Asn Val Val Ala Thr Thr Ser
100 105 110
Thr Thr Ser Ser Ser Thr Thr Thr Thr Thr Ala Thr Thr Thr Thr Ala
115 120 125
Thr Lys Thr Thr Ser Thr Thr Ser Ala Thr Ala Thr Pro Thr Ser Ser
130 135 140
Ala Thr Phe Pro Ser Gly Asn Ser Thr Ile Thr Thr Trp Ala Lys Ser
145 150 155 160
Gln Lys Asp Ile Ser Trp Lys Thr Leu Leu Thr Ser Leu Asn Pro Ser
165 170 175
Gly Thr Ala Lys Gly Phe Ile Ala Ala Ser Leu Ser Thr Ser Asn Pro
180 185 190
Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala Leu Val Ala Arg Thr
195 200 205
Met Val Asn Met Tyr Asn Thr Thr Glu Ala Gly Ser Ala Ser Val Leu
210 215 220
Gly Leu Leu Gln Asp Tyr Val Thr Phe Gln Ile Asn Ala Met Ser Thr
225 230 235 240
Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly
245 250 255
Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala
260 265 270
Glu Arg Ala Ser Thr Phe Ile Leu Phe Ala Asp Ser Tyr Ile Ala Gln
275 280 285
Gly Gly Gln Leu Ser Tyr Val Thr Gly Thr Leu Ala Pro Ala Ile Tyr
290 295 300
Lys Asp Leu Asn Tyr Val Val Ser Thr Trp Ser Asn Asn Cys Phe Asp
305 310 315 320
Leu Trp Glu Glu Val Asn Gly Arg His Met Phe Thr Leu Ala Val Met
325 330 335
Arg Arg Ala Leu Leu Asp Gly Val Asn Phe Ala Ser Arg Ile Gly Asp
340 345 350
Thr Thr Tyr Ser Ser Thr Trp Ser Ser Thr Ala Ser Ser Ile Gln Ser
355 360 365
Thr Leu Ser Gly Tyr Tyr Leu Ser Ser Gly Asn Tyr Ile Thr Thr Val
370 375 380
Gln Asn Phe Gln Ser Gly Val Ser Lys Ala Gly Tyr Asp Val Ser Thr
385 390 395 400
Leu Ile Ala Ala Asn Val Ala Gly Met Gly Asp Gly Phe Phe Thr Pro
405 410 415
Gly Ser Asp Glu Val Leu Ala Thr Ala Val Ala Ile Glu Asn Lys Phe
420 425 430
Ser Ser Leu Tyr Gly Val Asn Ala Asn Lys Ala Ser Tyr Leu Gly Thr
435 440 445
Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Tyr Gly Asn Gly
450 455 460
Gln Gly Asn Pro Trp Phe Ile Ala Thr Ala Ala Tyr Ala Glu Leu Tyr
465 470 475 480
Tyr Arg Ala Ile Leu Glu Trp Gln Thr Lys Ser Ser Ile Val Val Asn
485 490 495
Ser Lys Asn Leu Gly Phe Phe Ser Lys Phe Asp Ser Ser Ala Ala Val
500 505 510
Gly Thr Thr Tyr Thr Pro Gly Thr Thr Ala Tyr Ser Asn Met Val Gln
515 520 525
Asn Val Ala Leu Ala Ala Asp Arg Phe Leu Ser Thr Val Gln Leu His
530 535 540
Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Phe Asn Arg Asp Thr Gly
545 550 555 560
Val Met Gln Gly Ala Arg Asp Leu Thr Trp Ser His Ser Ala Phe Ile
565 570 575
Thr Ala Ala Arg Ala Lys Leu Gly Ser Pro Val Tyr
580 585
<210> 78
<211> 448
<212> PRT
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 78
Ala Asn Ser Thr Val Pro Asp Gly Asn Ser Thr Thr Thr Ala Trp Val
1 5 10 15
Lys Lys Gln Glu Ala Ile Ser Trp Thr Asp Leu Lys Thr Asn Val Asn
20 25 30
Pro Glu Gly Ala Ala Lys Gly Phe Ile Ala Ala Ser Leu Ser Thr Ser
35 40 45
Glu Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala Leu Val Ala
50 55 60
Arg Val Met Val Asn Lys Tyr Asn Thr Thr Asp Ala Gly Asp Ala Asn
65 70 75 80
Leu Leu Gly Leu Leu Gln Asp Tyr Val Ser Phe Gln Ile Asn Ala Met
85 90 95
Gly Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro
100 105 110
Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly
115 120 125
Pro Ala Glu Arg Ala Ser Thr Phe Ile Leu Leu Ala Asp Ser Met Ile
130 135 140
Ala Gln Lys Ser Ala Asn Gly Ser Tyr Val Ser Asp Thr Leu Ala Pro
145 150 155 160
Ala Ile Tyr Lys Asp Leu Ala Tyr Val Ala Ser Thr Trp Glu Asn Ala
165 170 175
Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Lys His Met Tyr Thr Leu
180 185 190
Ser Val Met Arg Arg Ala Leu Leu Asp Gly Ala Asp Phe Ala Ser Arg
195 200 205
Gln Gly Gln Thr Ala Asn Val Thr Ser Trp Lys Ser Thr Ala Asp Lys
210 215 220
Ile Lys Ser Ser Leu Glu Gly Phe Phe Ser Ser Asp Asn Gly Tyr Ile
225 230 235 240
Glu Val Thr Gln Asp Met Gln Gly Gly Val Gln Lys Lys Gly Leu Asp
245 250 255
Val Ser Thr Leu Ile Ala Ala Asn Ile Gly Ser Met Gly Asp Gly Phe
260 265 270
Tyr Thr Pro Gly Ser Asp Glu Val Leu Ala Thr Ala Val Ala Val Glu
275 280 285
Ala Ala Phe Ala Asp Leu Tyr Lys Ile Asn Gln Asn Thr Asn Ser Thr
290 295 300
Gly Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
305 310 315 320
Val Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Asn Thr Phe
325 330 335
Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Asn Lys Gly Thr
340 345 350
Ile Thr Val Asn Ser Val Asn Ala Ala Phe Phe Ser Lys Phe Asp Ser
355 360 365
Ser Ala Lys Ala Gly Thr Thr Tyr Lys Ser Gly Ser Thr Glu Phe Asp
370 375 380
Ser Leu Ile Asn Lys Val Ala Leu Ala Ala Asp Ala Phe Leu Asn Thr
385 390 395 400
Val Gln Thr Tyr Ala Ala Ser Asn Gly Ser Met Ser Glu Gln Tyr Asn
405 410 415
Arg Asp Thr Gly Ala Leu Thr Gly Ala Arg Asp Leu Thr Trp Ser His
420 425 430
Ala Ser Leu Ile Thr Ala Ala Asn Ala Lys Leu Gly Thr Pro Tyr Asn
435 440 445
<210> 79
<211> 452
<212> PRT
<213> Thermomucor属物种
<400> 79
Val Val Thr Ala Ala Ala Ala Ala Ser Val Pro Ser Gly Asn Ala Thr
1 5 10 15
Ile Thr Ser Trp Ile Gln Glu Gln Leu Ala Ile Ser Trp Asn Thr Met
20 25 30
Leu Thr Ser Val Asn Pro Val Gly Ala Val Thr Gly Phe Ile Ala Ala
35 40 45
Ser Leu Ser Thr Ala Asn Pro Asp Tyr Tyr Tyr Cys Trp Thr Arg Asp
50 55 60
Ala Ala Leu Val Ala Arg Val Met Thr Phe Met Tyr Asn Thr Thr Gln
65 70 75 80
Ala Gly Asp Ser Gly Leu Leu Gly Ile Leu Gln Asp Tyr Val Ser Phe
85 90 95
Gln Ile His Ala Met Gly Glu Ser Thr Val Cys Asn Cys Leu Gly Glu
100 105 110
Pro Lys Phe Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg
115 120 125
Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Ser Thr Phe Ile Lys Ile
130 135 140
Ala Asp Ser Tyr Leu Thr Gln Thr Gly Asp Val Ser Tyr Val Thr Asn
145 150 155 160
Thr Leu Lys Pro Ala Ile Tyr Glu Asp Leu Asp Tyr Ile Val Asn Val
165 170 175
Trp Gln Asn Thr Cys Phe Asp Leu Trp Glu Glu Val Tyr Gly Met His
180 185 190
Met Tyr Thr Leu Ala Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asp
195 200 205
Phe Ala Thr Arg Asn Gly Asp Thr Asp Lys Ala Ser Thr Tyr Thr Ser
210 215 220
Thr Ala Thr Ser Ile Gln Thr Arg Leu Ala Thr Phe Trp Ser Asp Ser
225 230 235 240
Asn Gly Tyr Ile Thr Val Thr Gln Asp Tyr Gln Ser Gly Val Ser Lys
245 250 255
Ala Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met
260 265 270
Asn Asp Gly Phe Phe Thr Pro Gly Ser Asp Glu Ile Leu Ala Thr Ala
275 280 285
Val Lys Val Glu Ala Ala Phe Ala Asn Leu Tyr Gly Ile Asn Ile Asn
290 295 300
Lys Ala Ser Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr
305 310 315 320
Tyr Asn Gly Asn Gly Asn Ser Glu Gly Asn Pro Trp Phe Ile Ala Thr
325 330 335
Ala Ala Phe Ala Glu Leu Tyr Tyr Arg Ala Ile Met Glu Trp Gln Leu
340 345 350
Arg Gly Ser Ser Ile Thr Val Asn Ser Val Asn Gln Gly Phe Phe Thr
355 360 365
Lys Phe Asp Pro Ser Ala Thr Ala Gly Thr Thr Tyr Thr Pro Gly Thr
370 375 380
Asp Ala Phe Asn Ser Leu Ile Asp Asn Val Ala Leu Ala Ala Asp Gln
385 390 395 400
Phe Phe Ser Thr Ile His Leu His Arg Ala Thr Asn Gly Ser Met Ser
405 410 415
Glu Gln Tyr Asn Arg Asp Thr Gly Phe Met Gln Gly Ala Arg Asp Leu
420 425 430
Thr Trp Ser His Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys Gln Gly
435 440 445
Thr Pro Ser Phe
450
<210> 80
<211> 589
<212> PRT
<213> Zychaea mexicana
<400> 80
Val Asp Val Pro Ser Val Pro Ile Gln Leu Glu Ser Tyr Thr Tyr Ser
1 5 10 15
Glu Asn Val Phe Ala Gly Arg Ile Phe Val Gln Asn Ile Ala Tyr Thr
20 25 30
Lys Glu Val Asn Val Phe Trp Ser Asp Ala Ser Asp Asp Trp Asn Asp
35 40 45
Asn Gly Asn Ser Val Ala Ala Ser Tyr Ser Glu Ser Ile Ala Asp Thr
50 55 60
Asn Tyr Glu Tyr Trp Glu Phe Ser Thr Thr Ile Gly Ser Ala Gly Ile
65 70 75 80
Ser Gln Ser Tyr Leu Arg Tyr Asp Val Ser Gly Ser Ile Tyr Tyr Asp
85 90 95
Asn Asn Asp Ser Gln Asn Tyr Asp Ile Thr Glu Thr Ser Thr Pro Thr
100 105 110
Thr Thr Thr Ala Ala Pro Thr Ser Thr Thr Ser Thr Pro Thr Thr Thr
115 120 125
Thr Asp Gly Gly Ser Thr Thr Thr Thr Thr Ala Thr Ser Val Pro Thr
130 135 140
Ser Thr Gly Val Pro Glu Gly Asn Ala Thr Ile Ser Glu Trp Ala Ser
145 150 155 160
Ala Gln Leu Asp Ile Ser Trp Pro Asn Leu Met Met Asn Val Asn Pro
165 170 175
Ser Gly Ala Val Thr Gly Ser Ile Val Ala Ser Leu Ser Thr Ser Asn
180 185 190
Pro Asp Tyr Phe Tyr Ile Trp Thr Arg Asp Ala Ala Met Val Ala Arg
195 200 205
Val Met Val Tyr Met Tyr Asn Thr Thr Glu Ala Gly Asp Thr Asn Leu
210 215 220
Leu Asn Ala Leu Thr Asp Tyr Val Thr Phe Ser Ile Ser Ser Met Asn
225 230 235 240
Val Asp Thr Val Cys Asp Cys Leu Gly Glu Pro Lys Phe Asn Val Asp
245 250 255
Gly Ser Gly Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro
260 265 270
Ala Glu Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Tyr Ile Ala
275 280 285
Gln Gly Gly Asp Val Ser Tyr Val Thr Asp Thr Leu Lys Pro Ala Ile
290 295 300
Tyr Thr Asp Leu Asp Tyr Val Val Asp Thr Trp Ser Asn Val Cys Phe
305 310 315 320
Asp Leu Trp Glu Glu Val Asn Gly Ile His Met Tyr Thr Leu Ser Val
325 330 335
Met Arg Lys Ala Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg Asn Gly
340 345 350
Asp Thr Ser Arg Val Ser Gly Tyr Glu Ser Thr Ala Ser Ser Ile Lys
355 360 365
Thr Arg Leu Glu Ser Phe Trp Ser Ser Ser Asn Asn Tyr Ile Thr Val
370 375 380
Thr Gln Ser Phe Ser Gly Gly Val Ser Lys Ala Gly Leu Asp Val Ser
385 390 395 400
Thr Leu Ile Ala Ala Asn Leu Ala Ser Met Asn Asp Gly Phe Tyr Thr
405 410 415
Pro Gly Ser Asp Glu Ile Leu Ala Thr Ala Val Ala Ile Glu Asn Ser
420 425 430
Phe Ile Ser Glu Tyr Thr Leu Asn Gln Asn Arg Pro Ser Trp Leu Ser
435 440 445
Thr Ala Ile Gly Arg Tyr Pro Glu Asp Ser Tyr Asp Gly Tyr Gly Asn
450 455 460
Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Ala Thr Tyr Ala Glu Leu
465 470 475 480
Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Gln Gln Ala Ser Ile Ser Val
485 490 495
Asn Ser Val Asn Leu Gly Phe Phe Ser Lys Phe Asp Ser Asp Ala Ser
500 505 510
Val Gly Thr Val Tyr Thr Pro Gly Thr Glu Asp Phe Ala Asn Met Val
515 520 525
Ser Asn Val Ala Phe Ala Ala Asp Glu Phe Leu Ala Thr Ile Glu Asn
530 535 540
His Ser Ala Val Asn Gly Ser Leu Ser Glu Gln Tyr Asn Arg Asp Thr
545 550 555 560
Gly Ile Met Gln Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala Phe
565 570 575
Ile Thr Ala Ala Lys Ala Lys Gln Gly Ala Pro Ile His
580 585
<210> 81
<211> 432
<212> PRT
<213> 瓶霉（Saksenaea vasiformis）
<400> 81
Trp Val Ser Lys Gln Glu Asp Ile Ser Phe Ser Glu Met Leu Arg Asn
1 5 10 15
Val Asn Pro Glu Gly Thr Ala Lys Gly Phe Val Ala Ala Ser Leu Ser
20 25 30
Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ala Ala Leu
35 40 45
Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr Asn Ala Gly Asp
50 55 60
Ser Lys Ile His Gly Val Leu Asp Asp Tyr Val Asn Phe Gln Ile Asn
65 70 75 80
Thr Gln Ser Glu Ser Thr Pro Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Ser Phe Met Leu Ile Ala Asp Ser
115 120 125
Phe Leu Ser Gln Thr Lys Asn Ala Ser Tyr Phe Thr Asn Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asp Thr Trp Ser Asn
145 150 155 160
Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Arg Ser Leu Leu Asp Gly Ala Asn Phe Ala Thr
180 185 190
Arg Asn Gly Asp Asn Ser Lys Ala Ser Thr Tyr Ser Gly Val Ala Ala
195 200 205
Lys Ile Gln Ala Arg Leu Asn Ser Phe Trp Asp Ala Gly Lys Asn Tyr
210 215 220
Ile Thr Val Thr Gln Asp Tyr Lys Asn Gly Val Glu Lys Pro Ser Gly
225 230 235 240
Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Gly Asp
245 250 255
Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala Thr Ala Leu Ala
260 265 270
Phe Glu Lys Ser Met Ala Ser Leu Tyr Pro Leu Asn Asn Asn Leu Pro
275 280 285
Ser His Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn
290 295 300
Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala
305 310 315 320
Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Lys Asn Thr Gly
325 330 335
Val Thr Val Thr Pro Ile Ser Lys Asp Phe Phe Val Arg Phe Asp Ser
340 345 350
Ser Ala Ala Pro Gly Lys Lys Tyr Asn Pro Gly Ser Gln Glu Phe Ala
355 360 365
Thr Leu Thr Gln Ser Ile Ala Ala Ala Ala Asp Arg Phe Met Ser Thr
370 375 380
Val Gln Tyr His Gln Asn Pro Asn Gly Ser Leu Ser Glu Glu Phe Asp
385 390 395 400
Arg Ser Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly Ser Pro Ser Phe
420 425 430
<210> 82
<211> 432
<212> PRT
<213> 螺旋巴克斯霉（Backusella circina）
<400> 82
Trp Ser Pro Ser Gln His Ser Ile Ser Leu Tyr Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Pro Gly Ser Ala Ala Gly Phe Ile Ser Ala Ser Leu Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu
35 40 45
Val Ala His Val Ile Val Asn Glu Tyr Asn Thr Thr Tyr Gln Gly Asn
50 55 60
Ser Thr Leu Leu Gly Ile Leu Lys Asp Tyr Val Thr Tyr Ser Leu Asn
65 70 75 80
Ala Gln Thr Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Phe Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Val Ser Phe Ile Tyr Phe Ala Asp Ser
115 120 125
Tyr Leu Thr Gln Thr Ser Asp Ser Ser Tyr Val Thr Gly Thr Leu Ala
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Ser Val Trp Ser Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr
165 170 175
Leu Met Phe Met Arg Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Ser
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Thr Ser Thr Ala Ala
195 200 205
Ser Ile Lys Thr Lys Ile Asp Gly Phe Trp Val Ser Ser Gly Asn Tyr
210 215 220
Val Gln Val Ser Gln Ser Val Thr Ser Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Ala Ser Thr Leu Ile Ala Ala Asn Gln Ala Ser Arg Gly Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Ser Gln Phe Ser Ser Leu Tyr Ser Ile Asn Thr Asn Lys Ala Ser
275 280 285
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Cys Thr Asn Ala Phe
305 310 315 320
Gly Glu Leu Phe Tyr Arg Ala Ile Ser Glu Trp Asn Ala Ala Gly Ser
325 330 335
Val Thr Val Asn Ser Val Asn Leu Ala Phe Phe Lys Lys Tyr Asp Ser
340 345 350
Ser Thr Ser Ser Gly Thr Thr Tyr Thr Val Gly Thr Ser Ala Tyr Asn
355 360 365
Asn Leu Val Gln Asn Val Ala Leu Ala Ala Asp Ala Tyr Phe Ser Thr
370 375 380
Val Lys Tyr His Ala Leu Thr Asn Gly Ser Met Ser Glu Gln Tyr Asp
385 390 395 400
Arg Ser Ser Gly Met Ala Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Met Ile Thr Ala Leu Lys Ala Lys Ser Gly Thr Pro Val Tyr
420 425 430
<210> 83
<211> 432
<212> PRT
<213> 螺旋巴克斯霉（Backusella circina）
<400> 83
Trp Ile Lys Gly Gln Glu Asp Thr Ser Arg Ser Val Met Leu Gly Asn
1 5 10 15
Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe Ile Ser Ala Ser Leu Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Tyr Tyr His Trp Thr Arg Asp Ala Ala Leu
35 40 45
Val Ala His Val Ile Val Asn Asp Tyr Asn Thr Thr Leu Ser Gly Asp
50 55 60
Ser Ser Thr Leu Gln Val Ile Lys Asp Tyr Val Thr Phe Ser Val Asn
65 70 75 80
Ser Gln Ser Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe Ile Phe Phe Ala Asp Thr
115 120 125
Tyr Leu Ala Gln Gly Gly Asp Ser Ser Tyr Val Thr Gly Thr Leu Ala
130 135 140
Pro Ala Ile Tyr Ala Asp Leu Asp Tyr Val Val Asn Asn Trp Ser Thr
145 150 155 160
Gly Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Arg Gly Leu Ile Asp Gly Ala Ser Phe Ala Ser
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ser Ser Ser Tyr Thr Ser Thr Ala Lys
195 200 205
Ser Ile Ala Thr Lys Ile Asp Ser Phe Trp Ser Ala Ser Asn Asn Tyr
210 215 220
Val Ala Val Ser Gln Ser Val Thr Ser Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Ala Ser Thr Ile Ile Ala Ala Asn Gln Ala Ser Leu Gly Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ser Val Ala Val
260 265 270
Glu Asn Ala Phe Ser Ser Leu Tyr Thr Val Asn Thr Gly Lys Ala Ser
275 280 285
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Tyr Leu Cys Thr Asn Ala Leu
305 310 315 320
Gly Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Asn Ala Ala Gly Ser
325 330 335
Val Thr Val Asn Ser Val Asn Leu Gly Phe Phe Gln Lys Ile Asp Ser
340 345 350
Ser Ile Ser Ser Gly Thr Thr Phe Thr Leu Gly Thr Ser Asp Tyr Ser
355 360 365
Thr Leu Val Asp Asn Val Ala Leu Ala Ala Asp Lys Phe Phe Ala Val
370 375 380
Val Gln Tyr His Glu Arg Ser Asn Gly Ser Met Pro Glu Gln Phe Gly
385 390 395 400
Arg Glu Asp Gly Leu Pro Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Met Ile Ser Ala Ala Arg Ala Lys Ala Gly Thr Pro Val Tyr
420 425 430
<210> 84
<211> 432
<212> PRT
<213> Benjaminiella poitrasii
<400> 84
Trp Leu Lys Ser Gln Ile Gly Ile Ser Arg Tyr Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Ala Gly Ser Ala Val Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ala Asn Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala Leu
35 40 45
Thr Ser Tyr Val Ile Ala Asn Asp Tyr Asn Ser Thr Leu Ala Gly Asn
50 55 60
Ser Thr Ile Leu Gln Ile Met Lys Asp Tyr Val Thr Phe Ser Val Lys
65 70 75 80
Ser Gln Ser Val Ser Thr Ala Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe Ile Leu Phe Ala Asp Ser
115 120 125
Tyr Leu Lys Gln Thr Gly Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys
130 135 140
Pro Ala Ile Phe Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Thr Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Lys Gly Leu Ile Arg Gly Ala Asn Phe Ala Thr
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Thr Asn Thr Ala Ala
195 200 205
Ser Ile Lys Thr Lys Met Asp Ser Phe Trp Ser Ser Gly Asn Asn Tyr
210 215 220
Ile Ala Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Val Ala Asn Ile Ile Ala Ala Asn Val Gly Ser Leu Gln Asp Gly
245 250 255
Val Tyr Thr Pro Gly Ser Asp Arg Ile Leu Ala Thr Ala Val Ala Val
260 265 270
Glu Ala Lys Phe Ala Ser Leu Tyr Gly Val Asn Ser Asn Leu Pro Gly
275 280 285
Tyr Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Asn Ala Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Arg Glu Trp Tyr Asp Asn Gly Gly
325 330 335
Val Thr Val Asn Ser Val Asn Leu Pro Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Ala Ser Gly Thr Thr Tyr Thr Val Gly Thr Thr Ala Phe Asn
355 360 365
Thr Met Val Ser Asn Val Ala Ala Ala Ala Asp Lys Phe Phe Ser Thr
370 375 380
Val Lys Phe His Ala Tyr Thr Asn Gly Ser Met Ser Glu Gln Phe Gly
385 390 395 400
Arg Asn Asp Gly Leu Cys Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Ser Ala Ala Leu Ala Lys Ala Gly Thr Pro Ser Val
420 425 430
<210> 85
<211> 432
<212> PRT
<213> 瓜笄霉（Choanephora cucurbitarum）
<400> 85
Trp Ile Asp Lys Gln Ile Asp Ile Ser Arg Ser Ala Met Leu Lys Asn
1 5 10 15
Ile Asn Pro Ala Gly Thr Val Lys Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Asn Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ala Ala Leu
35 40 45
Val Ala His Val Val Ala Asn Asp Tyr Asn Arg Thr Lys Ser Gly Asp
50 55 60
Ala Thr Tyr Leu Gly Leu Leu Lys Asp Tyr Val Thr Phe Ser Ile Asn
65 70 75 80
Ser Gln Asn Thr Pro Thr Ala Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Lys Asp Gly Ser Gly Tyr Asn Gly Pro Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Asp Thr Phe Val Leu Ile Ala Asp Ser
115 120 125
Ile Leu Thr Gln Thr Lys Asp Val Ser Tyr Val Thr Gly Thr Leu Arg
130 135 140
Pro Ala Ile Tyr Thr Asp Leu Asp Tyr Val Val Arg Thr Trp Ser Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Arg Ala Leu Leu Val Gly Ala Asn Phe Ala Ser
180 185 190
Arg Asn Gly Asp Ser Ala Arg Ala Ser Asn Tyr Asn Asn Ala Ala Asn
195 200 205
Ser Ile Lys Ser Lys Ile Asp Ser Phe Trp Ser Ser Asn Asn Asn Tyr
210 215 220
Val Ala Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Val Ser Thr Leu Ile Ala Ala Asn Val Gly Ser Leu Ser Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Glu Arg Met Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Asp Lys Phe Ala Asn Leu Tyr Gly Ile Asn Arg Asn Leu Asp Ser
275 280 285
Ser Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Asn Ala Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Asn Asn Asn Gly Gly
325 330 335
Val Thr Val Thr Asn Val Asn Leu Asn Phe Phe Lys Lys Phe Asp Gly
340 345 350
Ser Ala Ser Val Gly Thr Lys Tyr Thr Ala Gly Ser Ala Ala Tyr Asn
355 360 365
Thr Leu Thr Gln Asn Ile Ala Leu Ala Ala Asp Lys Phe Phe Asn Thr
370 375 380
Val Lys Val His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr His
385 390 395 400
Arg Asp Thr Gly Ser Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Thr Ala Ala Leu Ala Lys Ala Gly Thr Pro Val Ala
420 425 430
<210> 86
<211> 432
<212> PRT
<213> 匍枝根霉（Rhizopus stolonifer）
<400> 86
Trp Ile Lys Arg Gln Glu Lys Ile Ser Arg Phe Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Pro Gly Ser Ala Ala Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu
35 40 45
Thr Ser Asn Leu Ile Ala Tyr Glu Tyr Asn Thr Thr Leu Ser Gly Asn
50 55 60
Thr Thr Ile Leu Asn Ile Leu Lys Asp Tyr Val Lys Phe Ser Ile Ser
65 70 75 80
Ser Gln Thr Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn His Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Asn Thr Phe Ile Leu Phe Ala Asp Ser
115 120 125
Tyr Leu Asp Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys
130 135 140
Pro Ala Ile Phe Lys Asp Leu Asp Tyr Val Val Asn Val Trp Ser Asn
145 150 155 160
Gly Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Lys Gly Leu Leu Leu Gly Ala Asp Phe Ala Lys
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Thr Asn Thr Ala Ser
195 200 205
Thr Ile Ala Thr Lys Ile Ser Ser Phe Trp Val Ser Ser Ser Asn Trp
210 215 220
Ile Gln Val Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Leu Ala Ala Asn Leu Gly Ser Val Glu Asp Gly
245 250 255
Phe Phe Thr Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Asp Ala Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu Pro Ser
275 280 285
Tyr Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn Gly Phe
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Val Asn Asn Gly Ser
325 330 335
Val Thr Val Ser Asn Ile Ser Leu Ser Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Thr Ala Gly Lys Thr Tyr Thr Ala Gly Thr Ala Asp Phe Asn
355 360 365
Asn Leu Ala Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu Ser Thr
370 375 380
Val Gln Thr His Ala Phe Asn Asn Gly Ser Leu Ala Glu Glu Tyr Asp
385 390 395 400
Arg Thr Thr Gly Val Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Thr Ala Ala Tyr Ala Lys Ala Gly Ser Pro Ala Ala
420 425 430
<210> 87
<211> 432
<212> PRT
<213> 卷枝毛霉（Mucor circinelloides）
<400> 87
Trp Ile Asp Gly Gln Thr Ser Val Ser Arg Tyr Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Ala Gly Ala Val Ser Gly Phe Ile Ala Ala Ser Met Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ser Ala Leu
35 40 45
Thr Ser His Val Val Ala Tyr Asp Tyr Asn Thr Thr Leu Ala Gly Asn
50 55 60
Ser Thr Ile Leu Gly Leu Leu Lys Asn Tyr Val Thr Phe Ser Ile Asn
65 70 75 80
Ser Gln Thr Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Lys Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Ser Arg Ala Asp Thr Phe Ile Leu Ile Ala Asp Ser
115 120 125
Ile Leu Lys Gln Thr Gly Asp Ala Thr Tyr Val Thr Gly Thr Leu Ala
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Ser Thr Trp Ser Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Arg Gly Leu Ile Lys Gly Ala Asn Phe Ala Ser
180 185 190
Arg Asn Gly Asp Asn Thr Arg Ala Asn Thr Tyr Thr Asn Thr Ala Ala
195 200 205
Ser Ile Lys Thr Lys Ile Asp Ser Phe Trp Asn Ser Asn Gly Asn Tyr
210 215 220
Val Thr Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Ala Ser Val Leu Ile Ala Ala Asn Leu Gly Ser Val Gln Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Ser Lys Phe Ala Ser Leu Tyr Ser Ile Asn Gln Asn Leu Asn Gly
275 280 285
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Cys Thr Asn Ala Phe
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Phe Asn Asn Gly Gly
325 330 335
Val Thr Val Thr Ser Ile Ser Leu Asn Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Ala Val Gly Thr Lys Tyr Thr Val Gly Thr Ser Ala Phe Asn
355 360 365
Ser Leu Val Gln Asn Val Ala Val Ala Ala Asp Ala Phe Phe Ser Thr
370 375 380
Val Lys Phe His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr Gly
385 390 395 400
Arg Ser Asp Gly Leu Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Ser Ala Ser Tyr Ala Lys Ala Gly Ser Pro Ala Ala
420 425 430
<210> 88
<211> 431
<212> PRT
<213> Dichotomocladium elegans
<400> 88
Trp Ala Asp Ser Gln His Lys Ile Ser Trp Lys Ala Met Leu Ala Asn
1 5 10 15
Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ala Ala Met
35 40 45
Val Ala His Val Ile Val Asn Ala Tyr Asn Thr Thr Lys Ala Gly Asp
50 55 60
Ala Thr Thr Leu Gly Val Leu Lys Asp Phe Val Thr Phe Gln Ile Lys
65 70 75 80
Ala Met Ala Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Thr Thr Phe Ile Leu Phe Ala Asp Ser
115 120 125
Tyr Leu Ser Gln Thr Gly Asp Ala Ala Tyr Val Thr Thr Leu Lys Arg
130 135 140
Ala Ile Phe Thr Asp Leu Asp Tyr Val Val Thr Thr Trp Gln Asp Asn
145 150 155 160
Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Leu His Met Tyr Thr Leu
165 170 175
Ala Val Met Arg Arg Ser Leu Val Asp Gly Ala Ser Phe Ala Ala Arg
180 185 190
Asn Gly Asp Asn Thr Arg Ala Ser Thr Tyr Thr Asn Thr Ala Lys Ser
195 200 205
Ile Glu Thr Lys Leu Ala Ser Phe Tyr Asn Ser Ser Ser Asn Tyr Val
210 215 220
Val Val Thr Lys Asn Phe Ala Gly Gly Val Asn Lys Ala Gly Leu Asp
225 230 235 240
Thr Ser Thr Leu Ile Ala Ala Asn Thr Ala Gly Leu Gly Asp Gly Phe
245 250 255
Phe Thr Pro Gly Ser Pro Glu Ile Leu Ala Thr Ala Ala Ala Ile Glu
260 265 270
Lys Ser Phe Ala Asp Leu Tyr Gly Ile Asn Lys Gln Ile Pro Asn Trp
275 280 285
Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn
290 295 300
Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Cys Thr Ala Thr Phe Ala
305 310 315 320
Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln His Ala Gly Ser Val
325 330 335
Thr Val Asn Asn Val Asn Leu Pro Phe Phe Lys Lys Phe Asp Ser Ser
340 345 350
Thr Ser Ser Gly Thr Thr Tyr Thr Val Gly Thr Ser Ser Phe Asp Ser
355 360 365
Leu Ile Ser Lys Val Ala Tyr Ala Ala Asp Asn Phe Phe Ser Thr Ile
370 375 380
Lys Tyr His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Phe Asn Arg
385 390 395 400
Asp Thr Gly Phe Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala
405 410 415
Ala Phe Ile Thr Ala Ala Lys Ala Lys Ala Gly Thr Pro Val Tyr
420 425 430
<210> 89
<211> 432
<212> PRT
<213> Fennellomyces属物种
<400> 89
Trp Val Asn Asp Gln Leu Glu Ile Ser Trp Pro Ser Leu Leu Lys Asn
1 5 10 15
Val Asn Pro Ser Gly Ala Val Thr Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Asn Asp Pro Asp Tyr Phe Tyr Cys Trp Thr Arg Asp Ala Ala Leu
35 40 45
Val Ala Arg Val Met Val Tyr Met Tyr Asn Thr Thr Glu Ala Gly Asp
50 55 60
Thr Ser Leu Arg Ser Lys Leu Gln Asp Tyr Val Thr Phe Gln Ile Asn
65 70 75 80
Ser Met Lys Thr Ala Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Lys Asp Gly Ser Gly Tyr Ser Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Asp Arg Ala Ile Thr Leu Ile Leu Phe Ala Asp Ser
115 120 125
Phe Ile Ala Gln Gly Gly Asp Val Ser Tyr Ile Thr Asn Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Thr Asn Leu Asp Tyr Val Val Asn Thr Trp Ser Asn
145 150 155 160
Val Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Ile Tyr Thr
165 170 175
Leu Ser Val Met Arg Lys Gly Leu Leu Glu Gly Ala Asp Phe Ala Ser
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ala Asn Thr Tyr Arg Ser Thr Ala Ser
195 200 205
Ser Ile Lys Thr Arg Leu Glu Ser Phe Trp Ser Ser Ser Asn Asn Tyr
210 215 220
Ile Thr Val Thr Gln Ser Tyr Ser Gly Gly Val Asn Lys Ala Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Leu Ala Ala Asn Gly Ala Ser Met Asn Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Asn Ser Phe Ala Gly Ile Tyr Ala Val Asn Gln Asn Arg Pro Asp
275 280 285
Trp Lys Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asp Gly
290 295 300
His Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Ala Ala Tyr
305 310 315 320
Ala Glu Met Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Gln Gln Pro Ser
325 330 335
Ile Thr Val Asn Ser Ile Asn Leu Ser Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Ala Val Gly Thr Val Tyr Lys Pro Gly Thr Gln Ala Phe Asn
355 360 365
Asn Met Val Ser Asn Val Ala Phe Ala Ala Asp Glu Phe Phe Ser Thr
370 375 380
Met Asn Phe His Ser Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr Asn
385 390 395 400
Arg Asn Thr Gly Ile Met Gln Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys Leu Gly Thr Pro Val Phe
420 425 430
<210> 90
<211> 432
<212> PRT
<213> 桃吉尔霉（Gilbertella persicaria）
<400> 90
Trp Leu Lys Gly Gln Ile Glu Thr Ser Arg Phe Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Ala Gly Thr Val Lys Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ala Asn Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ala Ala Leu
35 40 45
Val Gly His Val Ile Ala Asn Asp Tyr Asn Arg Thr Leu Ala Gly Asn
50 55 60
Ser Thr Tyr Leu Gly Leu Leu Lys Asp Tyr Val Thr Phe Ser Val Asn
65 70 75 80
Ser Gln Ser Thr Ser Thr Ile Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Lys Asp Gly Ser Gly Tyr Ser Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Asp Thr Phe Ile Leu Ile Ala Asp Ser
115 120 125
Ile Leu Lys Gln Thr Gly Asp Ala Thr Tyr Val Thr Gly Thr Leu Arg
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Val Trp Ser Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Arg Ser Leu Ile Leu Gly Ala Asn Phe Ala Ser
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ala Ser Thr Tyr Thr Asn Thr Ala Asn
195 200 205
Ser Ile Lys Thr Lys Ile Asp Thr Phe Trp Ser Ser Ser Asn Asn Tyr
210 215 220
Val Ala Val Ser Gln Ser Val Thr Gly Gly Val Asn Lys Ala Gly Tyr
225 230 235 240
Asp Val Ala Asn Leu Ile Ala Ala Asn Val Gly Ser Leu Asp Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala Thr Ala Val Ala Val
260 265 270
Glu Ser Lys Phe Ala Ser Ile Tyr Gly Val Asn Gln Asn Leu Pro Ser
275 280 285
Trp Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Asn Thr Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Thr Asn Asn Gly Gly
325 330 335
Val Thr Val Thr Asn Val Asn Phe Asn Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Ser Val Gly Thr Lys Tyr Thr Val Gly Thr Ser Ala Phe Asn
355 360 365
Thr Leu Thr Gln Asn Val Ala Leu Ala Ala Asp Asn Phe Phe Ser Thr
370 375 380
Val Lys Val His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Phe Gly
385 390 395 400
Arg Asp Ser Gly Val Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Thr Ala Ala Leu Ala Lys Thr Gly Ala Pro Val Ala
420 425 430
<210> 91
<211> 432
<212> PRT
<213> 卷枝毛霉（Mucor circinelloides）
<400> 91
Trp Ile Asp Gly Gln Thr Ser Val Ser Arg Tyr Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Ala Gly Ala Val Thr Gly Phe Ile Ala Ala Ser Met Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ser Ala Leu
35 40 45
Thr Ser His Val Val Ala Tyr Asp Tyr Asn Thr Thr Leu Ala Gly Asn
50 55 60
Ser Thr Ile Leu Gly Leu Leu Lys Asn Tyr Val Thr Phe Ser Leu Asn
65 70 75 80
Ser Gln Thr Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Lys Asp Gly Ser Gly Tyr Ser Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Ser Arg Ala Asp Thr Phe Ile Leu Ile Ala Asp Ser
115 120 125
Ile Leu Lys Gln Thr Gly Asp Ala Thr Tyr Val Thr Gly Thr Leu Ala
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Ser Thr Trp Ser Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Arg Gly Leu Val Lys Gly Ala Asn Phe Ala Ser
180 185 190
Arg Asn Gly Asp Ser Thr Arg Ala Thr Thr Tyr Thr Asn Thr Ala Ala
195 200 205
Ser Ile Lys Thr Lys Ile Asp Ser Phe Trp Asn Ser Asn Gly Gln Tyr
210 215 220
Val Ser Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Ala Ser Val Leu Ile Ala Ser Asn Leu Gly Ser Leu Gln Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Ser Lys Phe Ala Ser Leu Tyr Ser Ile Asn Gln Asn Leu Asn Gly
275 280 285
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Cys Thr Asn Ala Phe
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Phe Asn Asn Gly Gly
325 330 335
Val Thr Val Thr Ser Ile Ser Leu Asn Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Ala Val Gly Thr Lys Tyr Thr Val Gly Thr Ser Ser Phe Asn
355 360 365
Ser Leu Val Gln Asn Val Ala Val Ala Ala Asp Ala Phe Phe Ser Thr
370 375 380
Ile Lys Phe His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr Gly
385 390 395 400
Arg Thr Asp Gly Leu Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Ser Ala Ser Tyr Ala Lys Ala Gly Ser Pro Ala Ala
420 425 430
<210> 92
<211> 432
<212> PRT
<213> 伞形卷霉（Circinella umbellata）
<400> 92
Trp Val Asn Lys Gln Leu Lys Ile Ser Trp Ser Asp Leu Leu Gln Asn
1 5 10 15
Val Asn Pro Ser Gly Thr Val Thr Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Asn Pro Asp Tyr Phe Tyr Cys Trp Thr Arg Asp Ala Ala Met
35 40 45
Val Ala Arg Val Met Thr Tyr Met Tyr Asn Thr Thr Glu Ala Gly Asp
50 55 60
Ser Ser Leu Glu Ser Ala Leu Lys Asp Tyr Ile Thr Phe Gln Ile Asn
65 70 75 80
Ser Met Lys Thr Ala Thr Ala Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Thr Asp Gly Ser Gly Tyr Ser Gly Pro Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Thr Thr Met Ile Leu Phe Ala Asp Ser
115 120 125
Tyr Leu Ala Gln Gly Gly Asp Thr Ser Tyr Val Thr Asn Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Thr Asn Leu Asp Tyr Val Val Gly Thr Trp Ser Asn
145 150 155 160
Asn Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Ile Phe Thr
165 170 175
Leu Ala Val Met Arg Lys Ser Leu Leu Asp Gly Ala Asp Phe Ala Ala
180 185 190
Arg Asn Gly Asp Thr Ser Arg Val Ser Gly Tyr Gln Ser Thr Ala Ser
195 200 205
Ser Ile Lys Thr Lys Leu Glu Ser Phe Trp Ser Ser Ser Asn Asn Tyr
210 215 220
Ile Thr Val Thr Gln Ser Tyr Ser Gly Gly Val Gln Lys Ala Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Ile Ala Ala Asn Gln Gly Ser Met Gly Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Asn Ser Phe Ala Asn Ile Tyr Thr Ile Asn Lys Asn Lys Pro Ser
275 280 285
Trp Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Gly Gln Gly Asn Pro Trp Phe Ile Ala Thr Ala Thr Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Gln Gln Glu Ser
325 330 335
Val Thr Val Asn Ser Val Asn Phe Asp Phe Phe Ser Lys Phe Asp Ser
340 345 350
Ser Ala Lys Val Gly Thr Val Tyr Thr Pro Gly Thr Asp Thr Phe Asn
355 360 365
Thr Met Val Ser Asn Val Ala Phe Ala Ala Asp Glu Phe Leu Ser Thr
370 375 380
Met Glu His Tyr Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Phe Asn
385 390 395 400
Arg Glu Thr Gly Ser Leu Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Phe Ile Thr Ala Gly Lys Ala Lys Leu Gly Ile Pro Ser Phe
420 425 430
<210> 93
<211> 432
<212> PRT
<213> Mucor cordense
<400> 93
Trp Leu Lys Ser Gln Glu Asp Ile Ser Arg Gly Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Pro Gly Ala Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Gly Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala Leu
35 40 45
Thr Ser His Val Ile Ala His Asp Tyr Asn Thr Thr Leu Ala Gly Asn
50 55 60
Thr Thr Ile Leu Asn Ile Leu Lys Asp Tyr Val Thr Phe Ser Val Lys
65 70 75 80
Ser Gln Ser Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Thr Phe Ile Leu Phe Gly Asp Ser
115 120 125
Tyr Leu Lys Gln Thr Gly Asp Ala Thr Tyr Val Thr Gly Thr Leu Ala
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn
145 150 155 160
Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Ser Met Arg Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Lys
180 185 190
Arg Asn Gly Asp Thr Thr Arg Ala Thr Thr Tyr Thr Asn Thr Ala Ala
195 200 205
Ser Ile Ala Thr Lys Ile Asp Thr Phe Trp Val Ser Ser Gly Asn Tyr
210 215 220
Val Gln Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Cys Ala Asn Ile Leu Ala Ala Asn Met Ala Ala Asn His Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Ser Lys Ile Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Ser Lys Phe Ala Ser Leu Tyr Ser Ile Asn Ser Gly Leu Ala Ser
275 280 285
Trp Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Cys Thr Asn Ala Phe
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Thr Gln Ala Gly Ser
325 330 335
Val Thr Val Asp Ser Thr Ser Leu Asn Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Ala Ala Gly Thr Lys Tyr Thr Val Gly Thr Ser Ala Phe Thr
355 360 365
Asn Leu Val Gln Asn Ile Ala Asn Gly Ala Asp Lys Phe Leu Ser Thr
370 375 380
Ser Lys Phe His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr Asn
385 390 395 400
Arg Asp Ser Gly Leu Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Ser Ala Ser Arg Ala Lys Ala Gly Ser Pro Ser Leu
420 425 430
<210> 94
<211> 432
<212> PRT
<213> Phascolomyces articulosus
<400> 94
Trp Val Asn Thr Gln Phe Ala Ile Ser Trp Pro Thr Leu Leu Lys Asn
1 5 10 15
Val Asn Pro Ser Gly Thr Val Lys Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Asn Asn Pro Asp Tyr Phe Tyr Ser Trp Thr Arg Asp Ser Ala Leu
35 40 45
Val Ala His Thr Met Thr Tyr Leu Tyr Asn Thr Ser Glu Ala Gly Asp
50 55 60
Ser Thr Ile Glu Ser Ala Leu Lys Asp Tyr Val Thr Phe Ser Ile Asn
65 70 75 80
Ala Met Asn Ala Ala Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Thr Asp Gly Ser Gly Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Ser Arg Ala Thr Thr Met Ile Leu Phe Ala Asp Ser
115 120 125
Phe Leu Ala Gln Gly Gly Asp Val Ser Tyr Val Ile Asn Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Ser Thr Trp Ser Asn
145 150 155 160
Thr Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Val His Ile Tyr Thr
165 170 175
Leu Ser Val Met Arg Arg Ala Leu Ile Asp Gly Ala Asn Phe Ala Gln
180 185 190
Arg Asn Gly Asp Thr Ser Arg Val Ser Gly Tyr Thr Ser Thr Ala Thr
195 200 205
Thr Ile Lys Thr Arg Leu Glu Ser Phe Trp Ser Asp Ser Asn Asn Tyr
210 215 220
Ile Thr Val Thr Gln Ser Tyr Ser Gly Gly Val Gln Lys Ala Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Ile Ala Ala Asn Ile Gly Ser Val Gly Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Lys Val Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Lys Ser Phe Ala Asn Leu Tyr Thr Ile Asn Gln Asn Lys Pro Ser
275 280 285
Trp Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asp Gly
290 295 300
Tyr Gly Asn Ser Lys Gly Asn Pro Trp Phe Ile Ala Thr Ala Thr Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Gln Gln Ala Ser
325 330 335
Val Thr Val Asn Ser Ile Asn Leu Gly Phe Phe Ser Lys Phe Asp Ser
340 345 350
Ser Ala Ser Val Gly Thr Val Tyr Thr Pro Gly Thr Asp Ser Phe Ala
355 360 365
Asn Met Val Ser Asn Val Ala Phe Ala Ala Asp Glu Phe Leu Ser Thr
370 375 380
Ile Asp Tyr His Ala Met Asn Asn Gly Ser Met His Glu Gln Tyr Asn
385 390 395 400
Arg Asp Thr Gly Ile Ser Gln Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys Leu Gly Ala Pro Ala Phe
420 425 430
<210> 95
<211> 432
<212> PRT
<213> 小孢根霉（Rhizopus microsporus）
<400> 95
Trp Ile Lys Asn Gln Glu Glu Ile Ser Arg Phe Ala Met Leu Arg Asn
1 5 10 15
Ile Asn Pro Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ala Gly Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu
35 40 45
Thr Ala Asn Val Ile Ala Tyr Glu Tyr Asn Thr Thr Phe Ala Gly Asn
50 55 60
Thr Thr Leu Leu Lys Tyr Leu Lys Asp Tyr Val Thr Phe Ser Val Lys
65 70 75 80
Ser Gln Ser Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Ala Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Val Thr Phe Met Leu Ile Ala Asp Ser
115 120 125
Tyr Leu Thr Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys
130 135 140
Pro Ala Ile Phe Lys Asp Leu Asp Tyr Val Val Ser Val Trp Ser Asn
145 150 155 160
Gly Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Val Met Arg Lys Gly Leu Ile Leu Gly Ala Asp Phe Ala Ala
180 185 190
Arg Asn Gly Asp Ser Ser Arg Ala Ser Thr Tyr Lys Asn Thr Ala Ser
195 200 205
Thr Met Glu Ser Lys Ile Ser Ser Phe Trp Ser Asp Ser Asn Asn Tyr
210 215 220
Val Gln Val Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Leu Ala Ala Asn Ile Gly Ser Leu Pro Asp Gly
245 250 255
Phe Phe Thr Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Leu
260 265 270
Glu Asn Ala Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu Pro Ser
275 280 285
Tyr Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn Ala Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Ile Ser Asn Gly Lys
325 330 335
Val Thr Val Ser Asn Ile Ser Leu Pro Phe Phe Lys Lys Phe Asp Ser
340 345 350
Ser Ala Thr Ser Gly Lys Thr Tyr Thr Ala Gly Thr Ser Asp Phe Asn
355 360 365
Asn Leu Ala Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu Ser Thr
370 375 380
Val Lys Phe His Ala Tyr Asn Asn Gly Ser Leu Ser Glu Glu Tyr Asp
385 390 395 400
Arg Ser Thr Gly Met Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ser Leu Ile Thr Ala Ala Tyr Ala Lys Ala Gly Ser Pro Ala Ala
420 425 430
<210> 96
<211> 433
<212> PRT
<213> 伞菌霉（Spinellus fusiger）
<400> 96
Trp Gly Ser Lys Gln Asp Gly Ile Ser Phe Ser Thr Met Leu Gly Asn
1 5 10 15
Ile Asn Pro Pro Gly Ser Ser Lys Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ala Gly Pro Asn Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu
35 40 45
Val Ala Arg Ala Ile Thr Tyr Lys Tyr Ser Thr Ser Tyr Gln Asn Asp
50 55 60
Pro Lys Ile Leu Gly Leu Leu Lys Asp Tyr Val Thr Tyr Gln Val Asn
65 70 75 80
Glu Gln Thr Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Phe Ser Gly Pro Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Thr Met Ile Leu Phe Ala Lys Ser
115 120 125
Tyr Tyr Ala Gln Thr Asn Asp Val Gly Tyr Val Ser Asn Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Ile Val Asn Val Trp Gly Asn
145 150 155 160
Asn Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr
165 170 175
Leu Met Met Met Arg His Gly Leu Val Gln Gly Ser Ile Phe Ala Asn
180 185 190
Thr Leu Gly Asp Ser Thr Arg Ala Asn Thr Tyr Lys Thr Ala Ala Gln
195 200 205
Asn Ile Lys Asn Arg Ile Asp Thr Phe Trp Glu Ser Gly Ser Asn Tyr
210 215 220
Ile Val Val Thr Gln Asn Gln Ser Ala Gly Val Asn Lys Pro Ser Gly
225 230 235 240
Leu Asp Val Ala Val Leu Leu Ala Ala Asn Gln Gly Gly Leu Gly Asp
245 250 255
Gly Val Tyr Thr Pro Gly Ser Asp Lys Val Leu Ala Thr Ala Val Ala
260 265 270
Leu Glu Lys Ser Phe Ala Ser Leu Tyr Pro Ile Asn Lys Asn Leu Pro
275 280 285
Ser Tyr Tyr Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn
290 295 300
Gly Asn Gly Asn Ser Glu Ala Asn Pro Trp Phe Ile Ala Thr Thr Thr
305 310 315 320
Tyr Ala Glu Leu Tyr Tyr Arg Ala Ile Ser Glu Trp Thr Asn Gly Ser
325 330 335
Gly Val Thr Val Asn Ser Ile Asn Lys Glu Phe Phe Ser Lys Phe Asp
340 345 350
Ala Ser Ala Thr Asn Gly Lys Val Tyr Thr Pro Gly Ser Asp Ser Phe
355 360 365
Asn Ser Leu Val Asn Asn Val Ala Ile Ala Ala Asp Asn Phe Leu Ser
370 375 380
Thr Val Arg Tyr His Gln Thr Ser Asn Gly Ser Leu Ser Glu Gln Phe
385 390 395 400
Asn Arg Tyr Thr Gly Phe Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
405 410 415
His Ala Ala Met Val Thr Ala Leu Ala Ala Lys Ala Gly Thr Pro Ser
420 425 430
Pro
<210> 97
<211> 432
<212> PRT
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 97
Trp Ala Lys Ser Gln Lys Asp Ile Ser Trp Lys Thr Leu Leu Thr Ser
1 5 10 15
Leu Asn Pro Ser Gly Thr Ala Lys Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Asn Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala Leu
35 40 45
Val Ala Arg Thr Met Val Asn Met Tyr Asn Thr Thr Glu Ala Gly Ser
50 55 60
Ala Ser Val Leu Gly Leu Leu Gln Asp Tyr Val Thr Phe Gln Ile Asn
65 70 75 80
Ala Met Ser Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Thr Phe Ile Leu Phe Ala Asp Ser
115 120 125
Tyr Ile Ala Gln Gly Gly Gln Leu Ser Tyr Val Thr Gly Thr Leu Ala
130 135 140
Pro Ala Ile Tyr Lys Asp Leu Asn Tyr Val Val Ser Thr Trp Ser Asn
145 150 155 160
Asn Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Arg His Met Phe Thr
165 170 175
Leu Ala Val Met Arg Arg Ala Leu Leu Asp Gly Val Asn Phe Ala Ser
180 185 190
Arg Ile Gly Asp Thr Thr Tyr Ser Ser Thr Trp Ser Ser Thr Ala Ser
195 200 205
Ser Ile Gln Ser Thr Leu Ser Gly Tyr Tyr Leu Ser Ser Gly Asn Tyr
210 215 220
Ile Thr Thr Val Gln Asn Phe Gln Ser Gly Val Ser Lys Ala Gly Tyr
225 230 235 240
Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Gly Asp Gly
245 250 255
Phe Phe Thr Pro Gly Ser Asp Glu Val Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Asn Lys Phe Ser Ser Leu Tyr Gly Val Asn Ala Asn Lys Ala Ser
275 280 285
Tyr Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Tyr Gly Asn Gly Gln Gly Asn Pro Trp Phe Ile Ala Thr Ala Ala Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Thr Lys Ser Ser
325 330 335
Ile Val Val Asn Ser Lys Asn Leu Gly Phe Phe Ser Lys Phe Asp Ser
340 345 350
Ser Ala Ala Val Gly Thr Thr Tyr Thr Pro Gly Thr Thr Ala Tyr Ser
355 360 365
Asn Met Val Gln Asn Val Ala Leu Ala Ala Asp Arg Phe Leu Ser Thr
370 375 380
Val Gln Leu His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Phe Asn
385 390 395 400
Arg Asp Thr Gly Val Met Gln Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ser Ala Phe Ile Thr Ala Ala Arg Ala Lys Leu Gly Ser Pro Val Tyr
420 425 430
<210> 98
<211> 434
<212> PRT
<213> 总状共头霉（Syncephalastrum racemosum）
<400> 98
Trp Val Lys Lys Gln Glu Ala Ile Ser Trp Thr Asp Leu Lys Thr Asn
1 5 10 15
Val Asn Pro Glu Gly Ala Ala Lys Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ser Glu Pro Asp Tyr Tyr Tyr Ala Trp Thr Arg Asp Ser Ala Leu
35 40 45
Val Ala Arg Val Met Val Asn Lys Tyr Asn Thr Thr Asp Ala Gly Asp
50 55 60
Ala Asn Leu Leu Gly Leu Leu Gln Asp Tyr Val Ser Phe Gln Ile Asn
65 70 75 80
Ala Met Gly Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Thr Phe Ile Leu Leu Ala Asp Ser
115 120 125
Met Ile Ala Gln Lys Ser Ala Asn Gly Ser Tyr Val Ser Asp Thr Leu
130 135 140
Ala Pro Ala Ile Tyr Lys Asp Leu Ala Tyr Val Ala Ser Thr Trp Glu
145 150 155 160
Asn Ala Cys Tyr Asp Leu Trp Glu Glu Val Asn Gly Lys His Met Tyr
165 170 175
Thr Leu Ser Val Met Arg Arg Ala Leu Leu Asp Gly Ala Asp Phe Ala
180 185 190
Ser Arg Gln Gly Gln Thr Ala Asn Val Thr Ser Trp Lys Ser Thr Ala
195 200 205
Asp Lys Ile Lys Ser Ser Leu Glu Gly Phe Phe Ser Ser Asp Asn Gly
210 215 220
Tyr Ile Glu Val Thr Gln Asp Met Gln Gly Gly Val Gln Lys Lys Gly
225 230 235 240
Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Ile Gly Ser Met Gly Asp
245 250 255
Gly Phe Tyr Thr Pro Gly Ser Asp Glu Val Leu Ala Thr Ala Val Ala
260 265 270
Val Glu Ala Ala Phe Ala Asp Leu Tyr Lys Ile Asn Gln Asn Thr Asn
275 280 285
Ser Thr Gly Leu Gly Thr Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
290 295 300
Asn Gly Val Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Asn
305 310 315 320
Thr Phe Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Asn Lys
325 330 335
Gly Thr Ile Thr Val Asn Ser Val Asn Ala Ala Phe Phe Ser Lys Phe
340 345 350
Asp Ser Ser Ala Lys Ala Gly Thr Thr Tyr Lys Ser Gly Ser Thr Glu
355 360 365
Phe Asp Ser Leu Ile Asn Lys Val Ala Leu Ala Ala Asp Ala Phe Leu
370 375 380
Asn Thr Val Gln Thr Tyr Ala Ala Ser Asn Gly Ser Met Ser Glu Gln
385 390 395 400
Tyr Asn Arg Asp Thr Gly Ala Leu Thr Gly Ala Arg Asp Leu Thr Trp
405 410 415
Ser His Ala Ser Leu Ile Thr Ala Ala Asn Ala Lys Leu Gly Thr Pro
420 425 430
Tyr Asn
<210> 99
<211> 433
<212> PRT
<213> Thermomucor属物种
<400> 99
Trp Ile Gln Glu Gln Leu Ala Ile Ser Trp Asn Thr Met Leu Thr Ser
1 5 10 15
Val Asn Pro Val Gly Ala Val Thr Gly Phe Ile Ala Ala Ser Leu Ser
20 25 30
Thr Ala Asn Pro Asp Tyr Tyr Tyr Cys Trp Thr Arg Asp Ala Ala Leu
35 40 45
Val Ala Arg Val Met Thr Phe Met Tyr Asn Thr Thr Gln Ala Gly Asp
50 55 60
Ser Gly Leu Leu Gly Ile Leu Gln Asp Tyr Val Ser Phe Gln Ile His
65 70 75 80
Ala Met Gly Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Pro Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Thr Phe Ile Lys Ile Ala Asp Ser
115 120 125
Tyr Leu Thr Gln Thr Gly Asp Val Ser Tyr Val Thr Asn Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Glu Asp Leu Asp Tyr Ile Val Asn Val Trp Gln Asn
145 150 155 160
Thr Cys Phe Asp Leu Trp Glu Glu Val Tyr Gly Met His Met Tyr Thr
165 170 175
Leu Ala Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asp Phe Ala Thr
180 185 190
Arg Asn Gly Asp Thr Asp Lys Ala Ser Thr Tyr Thr Ser Thr Ala Thr
195 200 205
Ser Ile Gln Thr Arg Leu Ala Thr Phe Trp Ser Asp Ser Asn Gly Tyr
210 215 220
Ile Thr Val Thr Gln Asp Tyr Gln Ser Gly Val Ser Lys Ala Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly
245 250 255
Phe Phe Thr Pro Gly Ser Asp Glu Ile Leu Ala Thr Ala Val Lys Val
260 265 270
Glu Ala Ala Phe Ala Asn Leu Tyr Gly Ile Asn Ile Asn Lys Ala Ser
275 280 285
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
290 295 300
Asn Gly Asn Ser Glu Gly Asn Pro Trp Phe Ile Ala Thr Ala Ala Phe
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Met Glu Trp Gln Leu Arg Gly Ser
325 330 335
Ser Ile Thr Val Asn Ser Val Asn Gln Gly Phe Phe Thr Lys Phe Asp
340 345 350
Pro Ser Ala Thr Ala Gly Thr Thr Tyr Thr Pro Gly Thr Asp Ala Phe
355 360 365
Asn Ser Leu Ile Asp Asn Val Ala Leu Ala Ala Asp Gln Phe Phe Ser
370 375 380
Thr Ile His Leu His Arg Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr
385 390 395 400
Asn Arg Asp Thr Gly Phe Met Gln Gly Ala Arg Asp Leu Thr Trp Ser
405 410 415
His Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys Gln Gly Thr Pro Ser
420 425 430
Phe
<210> 100
<211> 432
<212> PRT
<213> Zychaea mexicana
<400> 100
Trp Ala Ser Ala Gln Leu Asp Ile Ser Trp Pro Asn Leu Met Met Asn
1 5 10 15
Val Asn Pro Ser Gly Ala Val Thr Gly Ser Ile Val Ala Ser Leu Ser
20 25 30
Thr Ser Asn Pro Asp Tyr Phe Tyr Ile Trp Thr Arg Asp Ala Ala Met
35 40 45
Val Ala Arg Val Met Val Tyr Met Tyr Asn Thr Thr Glu Ala Gly Asp
50 55 60
Thr Asn Leu Leu Asn Ala Leu Thr Asp Tyr Val Thr Phe Ser Ile Ser
65 70 75 80
Ser Met Asn Val Asp Thr Val Cys Asp Cys Leu Gly Glu Pro Lys Phe
85 90 95
Asn Val Asp Gly Ser Gly Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn
100 105 110
Asp Gly Pro Ala Glu Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser
115 120 125
Tyr Ile Ala Gln Gly Gly Asp Val Ser Tyr Val Thr Asp Thr Leu Lys
130 135 140
Pro Ala Ile Tyr Thr Asp Leu Asp Tyr Val Val Asp Thr Trp Ser Asn
145 150 155 160
Val Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Met Tyr Thr
165 170 175
Leu Ser Val Met Arg Lys Ala Leu Leu Asp Gly Ala Asn Phe Ala Thr
180 185 190
Arg Asn Gly Asp Thr Ser Arg Val Ser Gly Tyr Glu Ser Thr Ala Ser
195 200 205
Ser Ile Lys Thr Arg Leu Glu Ser Phe Trp Ser Ser Ser Asn Asn Tyr
210 215 220
Ile Thr Val Thr Gln Ser Phe Ser Gly Gly Val Ser Lys Ala Gly Leu
225 230 235 240
Asp Val Ser Thr Leu Ile Ala Ala Asn Leu Ala Ser Met Asn Asp Gly
245 250 255
Phe Tyr Thr Pro Gly Ser Asp Glu Ile Leu Ala Thr Ala Val Ala Ile
260 265 270
Glu Asn Ser Phe Ile Ser Glu Tyr Thr Leu Asn Gln Asn Arg Pro Ser
275 280 285
Trp Leu Ser Thr Ala Ile Gly Arg Tyr Pro Glu Asp Ser Tyr Asp Gly
290 295 300
Tyr Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Ala Thr Ala Thr Tyr
305 310 315 320
Ala Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Gln Gln Gln Ala Ser
325 330 335
Ile Ser Val Asn Ser Val Asn Leu Gly Phe Phe Ser Lys Phe Asp Ser
340 345 350
Asp Ala Ser Val Gly Thr Val Tyr Thr Pro Gly Thr Glu Asp Phe Ala
355 360 365
Asn Met Val Ser Asn Val Ala Phe Ala Ala Asp Glu Phe Leu Ala Thr
370 375 380
Ile Glu Asn His Ser Ala Val Asn Gly Ser Leu Ser Glu Gln Tyr Asn
385 390 395 400
Arg Asp Thr Gly Ile Met Gln Gly Ala Arg Asp Leu Thr Trp Ser His
405 410 415
Ala Ala Phe Ile Thr Ala Ala Lys Ala Lys Gln Gly Ala Pro Ile His
420 425 430
<210> 101
<211> 609
<212> PRT
<213> 不明毛霉（Mucor ambiguus）
<400> 101
Met Lys Phe Ser Ser Leu Leu Lys Lys Pro Leu Leu Leu Ile Ala Gly
1 5 10 15
Ile Leu Ala Ala Thr Val Val Ala Glu Thr Val Pro Thr Thr Ala Glu
20 25 30
Val Lys Val Lys Ser Phe Thr Tyr Asp Gly Ser Thr Phe Ala Gly Gln
35 40 45
Ile Tyr Ile Lys Asn Ile Ala Tyr Thr Lys Thr Val Thr Val Ile Tyr
50 55 60
Ser Asp Gly Ser Asn Asn Trp Asn Asn Asn Gly Asn Thr Ile Ala Ala
65 70 75 80
Ser Tyr Ser Ala Ala Ile Ser Gly Ser Asn Tyr Glu Tyr Trp Thr Phe
85 90 95
Ser Ala Pro Val Ser Ser Ile Lys Gln Phe Tyr Val Lys Tyr Val Val
100 105 110
Ser Gly Thr Thr Tyr Tyr Asp Asn Asn Ser Ser Gly Asn Tyr Gln Val
115 120 125
Ser Thr Thr Thr Thr Thr Thr Thr Ala Pro Thr Thr Thr Thr Ser Gly
130 135 140
Gly Ser Ser Thr Thr Thr Gly Gly Ser Thr Thr Thr Ala Thr Ser Val
145 150 155 160
Pro Thr Ala Val Pro Ser Gly Phe Pro Thr Gly Asn Ser Thr Ile Ser
165 170 175
Ser Trp Ile Asp Gly Gln Thr Ser Val Ser Arg Tyr Ala Met Leu Arg
180 185 190
Asn Ile Asn Pro Ala Gly Thr Val Thr Gly Phe Ile Ala Ala Ser Met
195 200 205
Ser Thr Ser Gly Pro Asp Tyr Phe Tyr Ala Trp Thr Arg Asp Ser Ala
210 215 220
Leu Thr Ser His Val Val Ala Tyr Asp Tyr Asn Thr Thr Leu Ala Gly
225 230 235 240
Asn Ser Thr Ile Leu Gly Leu Leu Lys Asn Tyr Val Thr Phe Ser Leu
245 250 255
Asn Ser Gln Thr Thr Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys
260 265 270
Phe Asn Lys Asp Gly Ser Ser Tyr Thr Gly Ala Trp Gly Arg Pro Gln
275 280 285
Asn Asp Gly Pro Ala Ser Arg Ala Asp Thr Phe Ile Leu Ile Ala Asp
290 295 300
Ser Ile Leu Lys Gln Thr Gly Asp Ala Thr Tyr Val Thr Gly Thr Leu
305 310 315 320
Ala Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Ser Thr Trp Ser
325 330 335
Asn Gly Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr
340 345 350
Thr Leu Met Val Met Arg Arg Gly Leu Val Lys Gly Ala Ser Phe Ala
355 360 365
Ser Arg Asn Gly Asp Ser Thr Arg Ala Asn Thr Tyr Thr Asn Thr Ala
370 375 380
Ala Ser Ile Lys Thr Lys Ile Asp Ser Phe Trp Asn Ser Asn Gly Gln
385 390 395 400
Tyr Val Ser Val Ser Gln Ser Val Thr Gly Gly Val Ser Lys Ala Gly
405 410 415
Tyr Asp Ala Ser Val Leu Ile Ala Ser Asn Leu Gly Ser Leu Gln Asp
420 425 430
Gly Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala
435 440 445
Ile Glu Ser Lys Phe Ala Ser Leu Tyr Ser Ile Asn Gln Asn Leu Asn
450 455 460
Gly Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn
465 470 475 480
Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Ile Cys Thr Asn Ala
485 490 495
Phe Ala Glu Leu Tyr Tyr Arg Ala Ile Lys Glu Trp Phe Asn Asn Gly
500 505 510
Gly Val Thr Val Thr Ser Ile Ser Leu Asn Phe Phe Lys Lys Phe Asp
515 520 525
Ser Ser Ala Ala Val Gly Thr Lys Tyr Thr Val Gly Thr Ser Ala Phe
530 535 540
Asn Ser Leu Val Gln Asn Val Ala Val Ala Ala Asp Ala Phe Phe Ser
545 550 555 560
Thr Ile Lys Phe His Ala Ala Thr Asn Gly Ser Met Ser Glu Gln Tyr
565 570 575
Gly Arg Thr Asp Gly Leu Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
580 585 590
His Ala Ser Leu Ile Ser Ala Ser Tyr Ala Lys Ala Gly Ser Pro Ala
595 600 605
Ala
<210> 102
<211> 605
<212> PRT
<213> 小孢根霉（Rhizopus microsporus）
<400> 102
Met Lys Leu Met Asn Pro Ser Ile Lys Ala Cys Val Phe Phe Ile Leu
1 5 10 15
Ser Tyr Leu Ser Leu Leu Val Ser Ser Ala Ala Val Pro Thr Ser Ala
20 25 30
Ala Val Gln Val Glu Ser Tyr Lys Tyr Asp Gly Thr Thr Phe Ser Gly
35 40 45
Arg Ile Phe Val Lys Asn Ile Ala Tyr Ser Lys Val Val Thr Val Ile
50 55 60
Tyr Ser Asp Gly Ser Asp Asn Trp Asn Asn Asn Asn Asn Lys Ile Ser
65 70 75 80
Ala Ala Tyr Ser Glu Ala Ile Ser Gly Ser Asn Tyr Glu Tyr Trp Thr
85 90 95
Phe Ser Ala Lys Val Ser Gly Ile Lys Gln Phe Tyr Val Lys Tyr Glu
100 105 110
Val Ser Gly Ser Thr Tyr Tyr Asp Asn Asn Gly Thr Lys Asn Tyr Gln
115 120 125
Val Gln Ala Thr Ser Ala Thr Ser Thr Thr Ala Thr Ala Thr Thr Thr
130 135 140
Thr Thr Thr Ser Thr Pro Ala Thr Ser Thr Gly Pro Ala Asn Asn Ala
145 150 155 160
Pro Val Ser Phe Pro Thr Gly Asn Ser Thr Ile Ser Ser Trp Ile Lys
165 170 175
Lys Gln Glu Glu Ile Ser Arg Phe Ala Met Leu Arg Asn Ile Asn Pro
180 185 190
Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser Thr Ala Gly
195 200 205
Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu Thr Ala Asn
210 215 220
Val Ile Ala Tyr Glu Tyr Asn Thr Thr Phe Thr Gly Asn Thr Thr Leu
225 230 235 240
Leu Lys Tyr Leu Lys Asp Tyr Val Thr Phe Ser Val Lys Ser Gln Ser
245 250 255
Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Ala Asp
260 265 270
Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro
275 280 285
Ala Glu Arg Ala Val Thr Phe Met Leu Ile Ala Asp Ser Tyr Leu Thr
290 295 300
Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys Pro Ala Ile
305 310 315 320
Phe Lys Asp Leu Asp Tyr Val Val Ser Val Trp Ser Asn Gly Cys Tyr
325 330 335
Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Val
340 345 350
Met Arg Lys Gly Leu Ile Leu Gly Ala Asp Phe Ala Ala Arg Asn Gly
355 360 365
Asp Ser Ser Arg Ala Ser Thr Tyr Lys Lys Thr Ala Ser Thr Met Glu
370 375 380
Ser Lys Ile Ser Ser Phe Trp Ser Asp Ser Asn Asn Tyr Ile Gln Val
385 390 395 400
Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys Gly Leu Asp Val Ser
405 410 415
Thr Leu Leu Ala Ala Asn Ile Gly Ser Leu Pro Asp Gly Phe Phe Thr
420 425 430
Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Leu Glu Asn Ala
435 440 445
Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu Pro Ser Tyr Leu Gly
450 455 460
Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn
465 470 475 480
Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn Ala Tyr Ala Glu Leu
485 490 495
Tyr Tyr Arg Ala Ile Ser Glu Trp Ile Ser Asn Gly Lys Val Thr Val
500 505 510
Ser Asn Ile Ser Leu Pro Phe Phe Lys Lys Phe Asp Pro Ser Ala Thr
515 520 525
Ser Gly Lys Thr Tyr Thr Ala Gly Thr Ser Asp Phe Asp Asn Leu Ala
530 535 540
Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu Ser Thr Val Lys Phe
545 550 555 560
His Ala Tyr Thr Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Ser Thr
565 570 575
Gly Met Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu
580 585 590
Ile Thr Ala Ala Tyr Ala Lys Ala Gly Ser Pro Ala Ala
595 600 605
<210> 103
<211> 605
<212> PRT
<213> 单孢根霉菌（Rhizopus azygosporus）
<400> 103
Met Lys Leu Met Asn Pro Ser Met Lys Ala Cys Val Phe Phe Ile Leu
1 5 10 15
Ser Tyr Phe Ser Leu Leu Val Ser Ser Ala Ala Val Pro Thr Ser Ala
20 25 30
Ala Val Gln Val Glu Ser Tyr Asn Tyr Asp Gly Thr Thr Phe Ser Gly
35 40 45
Arg Ile Phe Val Lys Asn Ile Ala Tyr Ser Lys Val Val Thr Val Ile
50 55 60
His Ser Asp Gly Ser Asp Asn Trp Asn Asn Asn Asn Asn Lys Val Ser
65 70 75 80
Ala Ala Tyr Ser Glu Ala Ile Ser Gly Ser Asn Tyr Glu Tyr Trp Thr
85 90 95
Phe Ser Ala Lys Leu Ser Gly Ile Lys Gln Phe Tyr Val Lys Tyr Glu
100 105 110
Val Ser Gly Ser Thr Tyr Tyr Asp Asn Asn Gly Thr Lys Asn Tyr Gln
115 120 125
Val Gln Ala Thr Ser Ala Thr Ser Thr Thr Ala Thr Ala Thr Thr Thr
130 135 140
Thr Ala Thr Gly Thr Thr Thr Thr Ser Thr Gly Pro Thr Ser Thr Ala
145 150 155 160
Ser Val Ser Phe Pro Thr Gly Asn Ser Thr Ile Ser Ser Trp Ile Lys
165 170 175
Asn Gln Glu Glu Ile Ser Arg Phe Ala Met Leu Arg Asn Ile Asn Pro
180 185 190
Pro Gly Ser Ala Thr Gly Phe Ile Ala Ala Ser Leu Ser Thr Ala Gly
195 200 205
Pro Asp Tyr Tyr Tyr Ser Trp Thr Arg Asp Ser Ala Leu Thr Ala Asn
210 215 220
Val Ile Ala Tyr Glu Tyr Asn Thr Thr Phe Thr Gly Asn Thr Thr Leu
225 230 235 240
Leu Lys Tyr Leu Lys Asp Tyr Val Thr Phe Ser Val Lys Ser Gln Ser
245 250 255
Val Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Ala Asp
260 265 270
Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro
275 280 285
Ala Glu Arg Ala Val Thr Phe Met Leu Ile Ala Asp Ser Tyr Leu Thr
290 295 300
Gln Thr Lys Asp Ala Ser Tyr Val Thr Gly Thr Leu Lys Pro Ala Ile
305 310 315 320
Phe Lys Asp Leu Asp Tyr Val Val Ser Val Trp Ser Asn Gly Cys Tyr
325 330 335
Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr Thr Leu Met Val
340 345 350
Met Arg Lys Gly Leu Ile Leu Gly Ala Asp Phe Ala Ala Arg Asn Gly
355 360 365
Asp Ser Ser Arg Ala Ser Thr Tyr Lys Lys Thr Ala Ser Thr Met Glu
370 375 380
Ser Lys Ile Ser Ser Phe Trp Ser Asp Ser Asn Asn Tyr Ile Gln Val
385 390 395 400
Ser Gln Ser Val Thr Ala Gly Val Ser Lys Lys Gly Leu Asp Val Ser
405 410 415
Thr Leu Leu Ala Ala Asn Ile Gly Ser Leu Pro Asp Gly Phe Phe Thr
420 425 430
Pro Gly Ser Glu Lys Ile Leu Ala Thr Ala Val Ala Leu Glu Asn Ala
435 440 445
Phe Ala Ser Leu Tyr Pro Ile Asn Ser Asn Leu Pro Ser Tyr Leu Gly
450 455 460
Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asn Gly Asn
465 470 475 480
Ser Gln Gly Asn Pro Trp Phe Leu Ala Val Asn Ala Tyr Ala Glu Leu
485 490 495
Tyr Tyr Arg Ala Ile Lys Glu Trp Ile Ser Asn Gly Lys Val Thr Val
500 505 510
Ser Asn Ile Ser Leu Pro Phe Phe Lys Lys Phe Asp Ser Ser Ala Thr
515 520 525
Ser Gly Lys Thr Tyr Thr Ala Gly Thr Ser Asp Phe Asn Asn Leu Ala
530 535 540
Gln Asn Ile Ala Leu Gly Ala Asp Arg Phe Leu Ser Thr Val Lys Phe
545 550 555 560
His Ala Tyr Thr Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Ser Thr
565 570 575
Gly Met Ser Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ser Leu
580 585 590
Ile Thr Ala Ala Tyr Ala Lys Ala Gly Ser Pro Ala Ala
595 600 605
<210> 104
<211> 468
<212> PRT
<213> 瓶霉（Saksenaea vasiformis）
<400> 104
Met Pro Ser Trp Lys Thr Leu Phe Leu Leu Leu Gly Pro Ile Ala Thr
1 5 10 15
Ala Ala Ala Ala Pro Val Asp Lys Gln Ser Leu Pro Thr Gly Asn Ser
20 25 30
Thr Ile Ser Ser Trp Val Ser Lys Gln Glu Asp Ile Ser Phe Ser Glu
35 40 45
Met Leu Arg Asn Val Asn Pro Glu Gly Thr Ala Lys Gly Phe Val Ala
50 55 60
Ala Ser Leu Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg
65 70 75 80
Asp Ala Ala Leu Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr
85 90 95
Asn Ala Gly Asp Ser Lys Ile His Gly Val Leu Asp Asp Tyr Val Asn
100 105 110
Phe Gln Ile Asn Thr Gln Ser Glu Pro Thr Pro Cys Asn Cys Leu Gly
115 120 125
Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly
130 135 140
Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Phe Met Leu
145 150 155 160
Ile Ala Asp Ser Phe Leu Ser Gln Thr Lys Asn Ala Ser Tyr Phe Thr
165 170 175
Asn Thr Leu Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asp
180 185 190
Thr Trp Ser Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile
195 200 205
His Phe Tyr Thr Leu Met Val Met Arg Arg Ser Leu Leu Asp Gly Ala
210 215 220
Asn Phe Ala Thr Arg Asn Gly Asp Asn Ser Lys Ala Ser Thr Tyr Ser
225 230 235 240
Gly Val Ala Ala Lys Ile Gln Ala Arg Leu Asn Ser Phe Trp Asp Ala
245 250 255
Gly Lys Asn Tyr Ile Thr Val Thr Gln Asp Tyr Lys Asn Gly Val Glu
260 265 270
Lys Pro Ser Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala
275 280 285
Gly Met Gly Asp Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala
290 295 300
Thr Ala Leu Ala Phe Glu Lys Ser Met Ala Ser Leu Tyr Pro Leu Asn
305 310 315 320
Asn Asn Leu Pro Ser His Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu
325 330 335
Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu
340 345 350
Ala Thr Thr Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp
355 360 365
Lys Asn Thr Gly Val Thr Val Thr Pro Ile Ser Lys Asp Phe Phe Val
370 375 380
Arg Phe Asp Ser Ser Ala Ala Pro Gly Lys Lys Tyr Asn Pro Gly Ser
385 390 395 400
Gln Glu Phe Ala Thr Leu Thr Gln Ser Ile Ala Ala Ala Ala Asp Arg
405 410 415
Phe Met Ser Thr Val Gln Tyr His Gln Asn Pro Asn Gly Ser Leu Ser
420 425 430
Glu Glu Phe Asp Arg Ser Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu
435 440 445
Thr Trp Ser His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly
450 455 460
Ser Pro Ser Phe
465
<210> 105
<211> 640
<212> PRT
<213> 黑曲霉（Aspergillus niger）
<400> 105
Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly
1 5 10 15
Leu Ala Asn Val Ile Ser Lys Arg Ala Thr Leu Asp Ser Trp Leu Ser
20 25 30
Asn Glu Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala
35 40 45
Asp Gly Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser
50 55 60
Pro Ser Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser
65 70 75 80
Gly Leu Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr
85 90 95
Ser Leu Leu Ser Thr Ile Glu Asn Tyr Ile Ser Ala Gln Ala Ile Val
100 105 110
Gln Gly Ile Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly Leu
115 120 125
Gly Glu Pro Lys Phe Asn Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp
130 135 140
Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile
145 150 155 160
Gly Phe Gly Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Thr Ala Thr
165 170 175
Asp Ile Val Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln
180 185 190
Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser
195 200 205
Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser
210 215 220
Ala Phe Ala Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln
225 230 235 240
Ala Pro Glu Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Phe
245 250 255
Ile Leu Ala Asn Phe Asp Ser Ser Arg Ser Gly Lys Asp Ala Asn Thr
260 265 270
Leu Leu Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp
275 280 285
Ser Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu
290 295 300
Val Val Asp Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser
305 310 315 320
Asp Ser Glu Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Thr Tyr Tyr
325 330 335
Asn Gly Asn Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu
340 345 350
Tyr Asp Ala Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Val Thr
355 360 365
Asp Val Ser Leu Asp Phe Phe Lys Ala Leu Tyr Ser Asp Ala Ala Thr
370 375 380
Gly Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Asp Ala
385 390 395 400
Val Lys Thr Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala
405 410 415
Ala Ser Asn Gly Ser Met Ser Glu Gln Tyr Asp Lys Ser Asp Gly Glu
420 425 430
Gln Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr
435 440 445
Ala Asn Asn Arg Arg Asn Ser Val Val Pro Ala Ser Trp Gly Glu Thr
450 455 460
Ser Ala Ser Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ile Gly
465 470 475 480
Thr Tyr Ser Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr
485 490 495
Gly Gly Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr
500 505 510
Ser Thr Ser Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr Ser Thr
515 520 525
Ser Ser Thr Ser Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp
530 535 540
Leu Thr Ala Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser
545 550 555 560
Ile Ser Gln Leu Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser
565 570 575
Ala Asp Lys Tyr Thr Ser Ser Asp Pro Leu Trp Tyr Val Thr Val Thr
580 585 590
Leu Pro Ala Gly Glu Ser Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser
595 600 605
Asp Asp Ser Val Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val
610 615 620
Pro Gln Ala Cys Gly Thr Ser Thr Ala Thr Val Thr Asp Thr Trp Arg
625 630 635 640
<210> 106
<211> 631
<212> PRT
<213> 烟曲霉（Aspergillus fumigatus）
<400> 106
Met Pro Arg Leu Ser Tyr Ala Leu Cys Ala Leu Ser Leu Gly His Ala
1 5 10 15
Ala Ile Ala Ala Pro Gln Leu Ser Ala Arg Ala Thr Gly Ser Leu Asp
20 25 30
Ser Trp Leu Gly Thr Glu Thr Thr Val Ala Leu Asn Gly Ile Leu Ala
35 40 45
Asn Ile Gly Ala Asp Gly Ala Tyr Ala Lys Ser Ala Lys Pro Gly Ile
50 55 60
Ile Ile Ala Ser Pro Ser Thr Ser Glu Pro Asp Tyr Tyr Tyr Thr Trp
65 70 75 80
Thr Arg Asp Ala Ala Leu Val Thr Lys Val Leu Val Asp Leu Phe Arg
85 90 95
Asn Gly Asn Leu Gly Leu Gln Lys Val Ile Thr Glu Tyr Val Asn Ser
100 105 110
Gln Ala Tyr Leu Gln Thr Val Ser Asn Pro Ser Gly Gly Leu Ala Ser
115 120 125
Gly Gly Leu Ala Glu Pro Lys Tyr Asn Val Asp Met Thr Ala Phe Thr
130 135 140
Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr
145 150 155 160
Ala Leu Ile Asp Phe Gly Asn Trp Leu Ile Asp Asn Gly Tyr Ser Ser
165 170 175
Tyr Ala Val Asn Asn Ile Trp Pro Ile Val Arg Asn Asp Leu Ser Tyr
180 185 190
Val Ser Gln Tyr Trp Ser Gln Ser Gly Phe Asp Leu Trp Glu Glu Val
195 200 205
Asn Ser Met Ser Phe Phe Thr Val Ala Val Gln His Arg Ala Leu Val
210 215 220
Glu Gly Ser Thr Phe Ala Lys Arg Val Gly Ala Ser Cys Ser Trp Cys
225 230 235 240
Asp Ser Gln Ala Pro Gln Ile Leu Cys Tyr Met Gln Ser Phe Trp Thr
245 250 255
Gly Ser Tyr Ile Asn Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp
260 265 270
Ala Asn Thr Val Leu Ala Ser Ile His Thr Phe Asp Pro Glu Ala Gly
275 280 285
Cys Asp Asp Thr Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn
290 295 300
His Lys Val Tyr Thr Asp Ser Phe Arg Ser Val Tyr Ala Ile Asn Ser
305 310 315 320
Gly Ile Pro Gln Gly Ala Ala Val Ser Ala Gly Arg Tyr Pro Glu Asp
325 330 335
Val Tyr Tyr Asn Gly Asn Pro Trp Phe Leu Thr Thr Leu Ala Ala Ala
340 345 350
Glu Gln Leu Tyr Asp Ala Ile Tyr Gln Trp Lys Lys Ile Gly Ser Ile
355 360 365
Ser Ile Thr Ser Thr Ser Leu Ala Phe Phe Lys Asp Ile Tyr Ser Ser
370 375 380
Ala Ala Val Gly Thr Tyr Ala Ser Ser Thr Ser Thr Phe Thr Asp Ile
385 390 395 400
Ile Asn Ala Val Lys Thr Tyr Ala Asp Gly Tyr Val Ser Ile Val Gln
405 410 415
Ala His Ala Met Asn Asn Gly Ser Leu Ser Glu Gln Phe Asp Lys Ser
420 425 430
Ser Gly Leu Ser Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala
435 440 445
Phe Leu Thr Ala Asn Met Arg Arg Asn Gly Val Val Pro Ala Pro Trp
450 455 460
Gly Ala Ala Ser Ala Asn Ser Val Pro Ser Ser Cys Ser Met Gly Ser
465 470 475 480
Ala Thr Gly Thr Tyr Ser Thr Ala Thr Ala Thr Ser Trp Pro Ser Thr
485 490 495
Leu Thr Ser Gly Ser Pro Gly Ser Thr Thr Thr Val Gly Thr Thr Thr
500 505 510
Ser Thr Thr Ser Gly Thr Ala Ala Glu Thr Ala Cys Ala Thr Pro Thr
515 520 525
Ala Val Ala Val Thr Phe Asn Glu Ile Ala Thr Thr Thr Tyr Gly Glu
530 535 540
Asn Val Tyr Ile Val Gly Ser Ile Ser Glu Leu Gly Asn Trp Asp Thr
545 550 555 560
Ser Lys Ala Val Ala Leu Ser Ala Ser Lys Tyr Thr Ser Ser Asn Asn
565 570 575
Leu Trp Tyr Val Ser Val Thr Leu Pro Ala Gly Thr Thr Phe Glu Tyr
580 585 590
Lys Tyr Ile Arg Lys Glu Ser Asp Gly Ser Ile Val Trp Glu Ser Asp
595 600 605
Pro Asn Arg Ser Tyr Thr Val Pro Ala Ala Cys Gly Val Ser Thr Ala
610 615 620
Thr Glu Asn Asp Thr Trp Gln
625 630
<210> 107
<211> 567
<212> PRT
<213> 根状索孔菌（Fibroporia radiculosa）
<400> 107
Met Leu Phe Leu Leu Ala Ala Leu Gly Leu Ala Cys Ser Ala Ala Ala
1 5 10 15
Gln Ser Thr Ser Val Ser Ala Tyr Ile Ala Ser Glu Ser Pro Val Ala
20 25 30
Lys Ala Gly Val Leu Ala Asn Ile Gly Thr Glu Gly Ser Leu Ser Ser
35 40 45
Gly Ala Tyr Ser Gly Val Val Ile Ala Ser Pro Ser Thr Val Asn Pro
50 55 60
Asp Tyr Leu Tyr Thr Trp Val Arg Asp Ser Ser Leu Thr Phe Gln Ala
65 70 75 80
Leu Ile Asp Gln Tyr Val Tyr Gly Glu Asp Pro Thr Leu Arg Ser Leu
85 90 95
Ile Asp Glu Phe Ile Thr Ala Glu Ser Ile Leu Gln Gln Thr Thr Asn
100 105 110
Pro Ser Gly Thr Val Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn
115 120 125
Ile Asn Glu Thr Ala Phe Thr Gly Pro Trp Gly Arg Pro Gln Arg Asp
130 135 140
Gly Pro Ala Leu Arg Ser Thr Ala Ile Ile Thr Tyr Ala Thr Tyr Leu
145 150 155 160
Trp Asn Ser Gly Asn Thr Ser Tyr Val Ser Asp Ser Leu Trp Pro Ile
165 170 175
Ile Glu Leu Asp Leu Asn Tyr Ile Ala Thr Tyr Trp Asn Phe Ser Thr
180 185 190
Phe Asp Leu Trp Glu Glu Ile Asp Ser Ser Ser Phe Trp Thr Thr Ala
195 200 205
Val Gln His Arg Ala Leu Arg Gln Gly Ile Thr Phe Ala Asn Leu Ile
210 215 220
Gly Gln Thr Ser Pro Val Ser Asn Tyr Glu Thr Gln Ala Gly Asp Ile
225 230 235 240
Leu Cys Phe Leu Gln Thr Tyr Trp Asn Pro Thr Gly Asn Tyr Met Thr
245 250 255
Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ser Asn Thr Val Leu
260 265 270
Ala Ser Val His Thr Phe Asp Pro Asp Ala Gly Cys Asp Ser Thr Thr
275 280 285
Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val
290 295 300
Asp Ser Phe Arg Ser Leu Tyr Ala Ile Asn Asp Gly Ile Ala Ser Asp
305 310 315 320
Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Tyr Tyr Gly Gly
325 330 335
Asn Pro Trp Tyr Leu Cys Thr Phe Ala Val Ala Glu Gln Leu Tyr Asp
340 345 350
Ala Leu Ile Val Trp Ser Ser Gln Gly Tyr Leu Glu Ile Thr Asp Leu
355 360 365
Ser Leu Ala Phe Phe Gln Gln Phe Asp Ser Asp Val Gly Thr Gly Thr
370 375 380
Tyr Asp Ser Gly Ser Ser Thr Tyr Ser Thr Leu Thr Ser Ala Ile Arg
385 390 395 400
Thr Phe Ala Asp Gly Phe Val Leu Thr Asn Ala Lys Tyr Thr Pro Thr
405 410 415
Asn Gly Ser Leu Ser Glu Glu Tyr Thr Ser Ala Asp Gly Thr Pro Ile
420 425 430
Ser Ala Tyr Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Val Phe
435 440 445
Ala Ala Glu Ala Gly Thr Thr Tyr Gly Ser Trp Gly Ala Ala Gly Leu
450 455 460
Thr Val Pro Ser Thr Cys Thr Ser Gly Val Ala Val Thr Phe Glu Val
465 470 475 480
Asp Tyr Asp Thr Glu Tyr Gly Glu Asn Val Tyr Ile Thr Gly Ser Val
485 490 495
Asn Ala Leu Glu Asn Trp Ser Ala Thr Asn Ala Leu Ile Met Ser Ala
500 505 510
Ala Asp Tyr Pro Thr Trp Ser Ile Thr Val Tyr Leu Pro Pro Ser Thr
515 520 525
Thr Ile Gln Tyr Lys Tyr Leu Thr Gln Tyr Asn Gly Glu Val Thr Trp
530 535 540
Glu Asp Asp Pro Asn Asn Glu Ile Thr Thr Pro Ala Ser Gly Ser Met
545 550 555 560
Thr Gln Val Asp Ser Trp His
565
<210> 108
<211> 582
<212> PRT
<213> 轮枝样镰刀菌（Fusarium verticillioides）
<400> 108
Met Phe Thr Gln Ile Leu Tyr Gly Leu Thr Ala Leu Ser Ala Leu Gln
1 5 10 15
Gly Gln Val Thr Ala Ser Pro Gly Gly Ser Ser Leu Asp Arg Phe Ile
20 25 30
Ser Lys Glu Ala Asp Ile Ser Ile Lys Gly Val Leu Ala Asn Ile Gly
35 40 45
Ala Asp Gly Lys Arg Ala Gln Gly Ala Ala Pro Gly Ala Val Val Ala
50 55 60
Ser Pro Ser Arg Thr Asp Pro Asp Tyr Trp Tyr Thr Trp Thr Arg Asp
65 70 75 80
Ser Ala Leu Thr Tyr Lys Val Leu Val Glu Arg Phe Ile His Gly Asp
85 90 95
Lys Ser Leu Gln Arg Lys Ile Asp Glu Tyr Val Ser Ala Gln Ala Lys
100 105 110
Leu Gln Gly Val Thr Asn Pro Ser Gly Gly Pro Glu Ser Gly Gly Leu
115 120 125
Gly Glu Pro Lys Phe His Val Asn Leu Thr Ala Phe Thr Gly Ser Trp
130 135 140
Gly Arg Pro Gln Arg Asp Gly Pro Pro Leu Arg Ala Thr Ala Leu Thr
145 150 155 160
Leu Tyr Ala Asn Trp Leu Val Ser His Gly Asp Arg Ser Lys Ala Val
165 170 175
Asn Lys Val Trp Pro Val Ile Glu Lys Asp Leu Ala Tyr Thr Val Lys
180 185 190
Phe Trp Asn Arg Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser
195 200 205
Ser Phe Phe Thr Leu Ser Ala Ser His Arg Ala Leu Val Glu Gly Ala
210 215 220
Ala Leu Ala Lys Lys Leu Gly Lys Ser Cys Ser Asp Cys Ala Thr Asn
225 230 235 240
Ala Pro Arg Val Leu Cys Phe Met Gln Ser Phe Trp Thr Gly Ser Tyr
245 250 255
Ile Asp Ser Asn Ile Asn Val Asn Asp Gly Arg Lys Gly Leu Asp Ala
260 265 270
Asn Ser Ile Leu Ser Ser Ile His Thr Phe Asp Pro Ser Ser Lys Cys
275 280 285
Thr Asp Ser Thr Phe Gln Pro Cys Ser Ser Arg Ala Leu Ala Asn His
290 295 300
Lys Glu Val Val Asp Ser Phe Arg Ser Ile Tyr Gly Val Asn Lys Asn
305 310 315 320
Arg Gly Lys Gly Lys Ala Ala Ala Val Gly Arg Tyr Ser Glu Asp Val
325 330 335
Tyr Tyr Asp Gly Asn Pro Trp Tyr Leu Ala Thr Leu Ala Ala Ala Glu
340 345 350
Gln Leu Tyr Ala Ala Val Tyr Gln Trp Asn Lys Ile Gly Ser Ile Thr
355 360 365
Val Asp Ser Val Ser Leu Pro Phe Phe Ser Asp Leu Val Pro Lys Val
370 375 380
Ser Lys Gly Thr Tyr Arg Lys Asn Ser Lys Thr Tyr Lys Ala Ile Ile
385 390 395 400
Lys Ala Val Thr Ser Tyr Ala Asp Gly Phe Val Ala Val Val Gln Thr
405 410 415
Tyr Thr Pro Lys Asp Gly Ser Leu Ala Glu Gln Phe Asp Lys Ser Thr
420 425 430
Gly Thr Pro Lys Ser Ala Val His Leu Thr Trp Ser Tyr Ala Ser Phe
435 440 445
Val Gly Ala Ala Glu Arg Arg Thr Gly Val Val Pro Pro Ala Trp Gly
450 455 460
Glu Ser Asn Ala Asn Lys Val Pro Ala Val Cys Glu Ala Ala Pro Ala
465 470 475 480
Cys Asp Thr Thr Ile Thr Phe Asn Val Lys Asn Val Asp Val Thr Ser
485 490 495
Asp Gln Lys Val Tyr Ile Val Gly Gly Ile Thr Gln Leu Ser Asn Trp
500 505 510
Ala Pro Ala Asp Gly Ile Ala Leu Glu Glu Ser Thr Ser Thr Lys Gly
515 520 525
Leu Trp Thr Val Lys Val Lys Ile Pro Ser Asp Thr Ser Phe Glu Tyr
530 535 540
Lys Tyr Ile Lys Lys Thr Ser Asp Gly Thr Val Thr Trp Glu Ser Asp
545 550 555 560
Pro Asn Asn Ser Ala Ala Thr Gly Ser Lys Cys Gly Ser Ser Ser Thr
565 570 575
Ile Asn Asp Glu Trp Arg
580
<210> 109
<211> 576
<212> PRT
<213> 密褐褶菌（Gloeophyllum trabeum）
<400> 109
Met Tyr Arg Phe Leu Val Cys Ala Leu Gly Leu Leu Gly Thr Val Leu
1 5 10 15
Ala Gln Ser Val Asp Ser Tyr Val Gly Ser Glu Gly Pro Ile Ala Lys
20 25 30
Ala Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly
35 40 45
Ala Ala Ala Gly Val Val Val Ala Ser Pro Ser Lys Ser Asp Pro Asp
50 55 60
Tyr Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu
65 70 75 80
Ile Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Ser
85 90 95
Leu Ile Asp Ser Phe Val Ile Ala Glu Ala Asn Ile Gln Gln Val Ser
100 105 110
Asn Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe
115 120 125
Asn Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg
130 135 140
Asp Gly Pro Ala Leu Arg Ala Thr Ala Leu Ile Thr Tyr Gly Asn Trp
145 150 155 160
Leu Leu Ser Asn Gly Asn Thr Thr Trp Val Thr Ser Thr Leu Trp Pro
165 170 175
Ile Ile Gln Asn Asp Leu Asn Tyr Val Val Gln Tyr Trp Asn Gln Thr
180 185 190
Thr Phe Asp Leu Trp Glu Glu Val Asn Ser Ser Ser Phe Phe Thr Thr
195 200 205
Ala Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Lys
210 215 220
Ile Gly Gln Thr Ser Ser Val Ser Ser Tyr Thr Thr Gln Ala Ala Asn
225 230 235 240
Leu Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Thr Ser Gly Tyr Ile
245 250 255
Thr Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu
260 265 270
Leu Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Thr
275 280 285
Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr
290 295 300
Val Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Ile Ala Ser
305 310 315 320
Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly
325 330 335
Gly Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr
340 345 350
Asp Ala Leu Asn Val Trp Ala Ala Gln Gly Ser Leu Asn Val Thr Ser
355 360 365
Ile Ser Leu Pro Phe Phe Gln Gln Phe Ser Ser Ser Val Thr Ala Gly
370 375 380
Thr Tyr Ala Ser Ser Ser Thr Thr Tyr Thr Thr Leu Thr Ser Ala Ile
385 390 395 400
Lys Ser Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Gln Tyr Thr Pro
405 410 415
Ser Asn Gly Gly Leu Ala Glu Gln Phe Ser Arg Ser Asn Gly Ala Pro
420 425 430
Val Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala
435 440 445
Phe Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Val Gly
450 455 460
Leu Thr Val Pro Thr Ser Cys Ser Ser Asn Ser Gly Gly Gly Gly Gly
465 470 475 480
Ser Thr Val Ala Val Thr Phe Asn Val Asn Ala Gln Thr Val Trp Gly
485 490 495
Glu Asn Ile Tyr Ile Thr Gly Ser Val Asp Ala Leu Ser Asn Trp Ser
500 505 510
Pro Asp Asn Ala Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser
515 520 525
Ile Thr Val Asn Leu Pro Ala Ser Thr Ala Ile Gln Tyr Lys Tyr Ile
530 535 540
Arg Lys Asn Asn Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser
545 550 555 560
Ile Thr Thr Pro Ala Ser Gly Ser Val Thr Glu Asn Asp Thr Trp Arg
565 570 575
<210> 110
<211> 616
<212> PRT
<213> 草酸青霉菌（Penicillium oxalicum）
<400> 110
Met Arg Leu Thr Leu Leu Ser Gly Val Ala Gly Val Leu Cys Ala Gly
1 5 10 15
Gln Leu Thr Ala Ala Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro
20 25 30
Phe Ile His Lys Glu Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn
35 40 45
Leu Gly Gly Arg Gly Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe
50 55 60
Ile Ala Ser Pro Asn Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr
65 70 75 80
Arg Asp Ser Ala Leu Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp
85 90 95
Ser Arg Ala Lys Phe Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile
100 105 110
Arg Asp Tyr Val Ser Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro
115 120 125
Ser Gly Thr Leu Lys Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu
130 135 140
Ile Asp Leu Asn Pro Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp
145 150 155 160
Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu
165 170 175
Ile Ser His Gly Gln Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile
180 185 190
Ile Ala Asn Asp Leu Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly
195 200 205
Phe Asp Leu Trp Glu Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala
210 215 220
Val Gln His Arg Ala Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu
225 230 235 240
Gly Lys Ser Cys Asp Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys
245 250 255
Phe Leu Gln Ser Phe Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn
260 265 270
Thr Gln Ala Ser Arg Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser
275 280 285
Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln
290 295 300
Pro Cys Ser Ala Arg Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser
305 310 315 320
Phe Arg Ser Ile Tyr Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala
325 330 335
Ala Asn Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro
340 345 350
Trp Tyr Leu Ala Thr Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu
355 360 365
Tyr Gln Trp Asp Arg Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu
370 375 380
Ser Phe Phe Lys Asp Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser
385 390 395 400
Arg Asn Ser Lys Thr Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr
405 410 415
Ala Asp Gly Phe Ile Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly
420 425 430
Ser Leu Ala Glu Gln Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala
435 440 445
Asn Asp Leu Thr Trp Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg
450 455 460
Arg Asp Ala Val Val Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys
465 470 475 480
Val Pro Thr Thr Cys Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala
485 490 495
Pro Thr Ala Thr Phe Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp
500 505 510
Ile Val Pro Ile Thr Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu
515 520 525
Asn Val Phe Met Ser Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala
530 535 540
Lys Lys Gly Phe Pro Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn
545 550 555 560
Leu Trp Phe Ala Ser Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu
565 570 575
Tyr Lys Tyr Tyr Lys Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys
580 585 590
Gly Pro Asn Arg Val Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro
595 600 605
His Ser Asn Asp Val Trp Gln Phe
610 615
<210> 111
<211> 632
<212> PRT
<213> 里氏木霉（Trichoderma reesei）
<400> 111
Met His Val Leu Ser Thr Ala Val Leu Leu Gly Ser Val Ala Val Gln
1 5 10 15
Lys Val Leu Gly Arg Pro Gly Ser Ser Gly Leu Ser Asp Val Thr Lys
20 25 30
Arg Ser Val Asp Asp Phe Ile Ser Thr Glu Thr Pro Ile Ala Leu Asn
35 40 45
Asn Leu Leu Cys Asn Val Gly Pro Asp Gly Cys Arg Ala Phe Gly Thr
50 55 60
Ser Ala Gly Ala Val Ile Ala Ser Pro Ser Thr Ile Asp Pro Asp Tyr
65 70 75 80
Tyr Tyr Met Trp Thr Arg Asp Ser Ala Leu Val Phe Lys Asn Leu Ile
85 90 95
Asp Arg Phe Thr Glu Thr Tyr Asp Ala Gly Leu Gln Arg Arg Ile Glu
100 105 110
Gln Tyr Ile Thr Ala Gln Val Thr Leu Gln Gly Leu Ser Asn Pro Ser
115 120 125
Gly Ser Leu Ala Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu Leu
130 135 140
Thr Leu Lys Pro Phe Thr Gly Asn Trp Gly Arg Pro Gln Arg Asp Gly
145 150 155 160
Pro Ala Leu Arg Ala Ile Ala Leu Ile Gly Tyr Ser Lys Trp Leu Ile
165 170 175
Asn Asn Asn Tyr Gln Ser Thr Val Ser Asn Val Ile Trp Pro Ile Val
180 185 190
Arg Asn Asp Leu Asn Tyr Val Ala Gln Tyr Trp Asn Gln Thr Gly Phe
195 200 205
Asp Leu Trp Glu Glu Val Asn Gly Ser Ser Phe Phe Thr Val Ala Asn
210 215 220
Gln His Arg Ala Leu Val Glu Gly Ala Thr Leu Ala Ala Thr Leu Gly
225 230 235 240
Gln Ser Gly Ser Ala Tyr Ser Ser Val Ala Pro Gln Val Leu Cys Phe
245 250 255
Leu Gln Arg Phe Trp Val Ser Ser Gly Gly Tyr Val Asp Ser Asn Ile
260 265 270
Asn Thr Asn Glu Gly Arg Thr Gly Lys Asp Val Asn Ser Val Leu Thr
275 280 285
Ser Ile His Thr Phe Asp Pro Asn Leu Gly Cys Asp Ala Gly Thr Phe
290 295 300
Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Val Val Asp
305 310 315 320
Ser Phe Arg Ser Ile Tyr Gly Val Asn Lys Gly Ile Pro Ala Gly Ala
325 330 335
Ala Val Ala Ile Gly Arg Tyr Ala Glu Asp Val Tyr Tyr Asn Gly Asn
340 345 350
Pro Trp Tyr Leu Ala Thr Phe Ala Ala Ala Glu Gln Leu Tyr Asp Ala
355 360 365
Ile Tyr Val Trp Lys Lys Thr Gly Ser Ile Thr Val Thr Ala Thr Ser
370 375 380
Leu Ala Phe Phe Gln Glu Leu Val Pro Gly Val Thr Ala Gly Thr Tyr
385 390 395 400
Ser Ser Ser Ser Ser Thr Phe Thr Asn Ile Ile Asn Ala Val Ser Thr
405 410 415
Tyr Ala Asp Gly Phe Leu Ser Glu Ala Ala Lys Tyr Val Pro Ala Asp
420 425 430
Gly Ser Leu Ala Glu Gln Phe Asp Arg Asn Ser Gly Thr Pro Leu Ser
435 440 445
Ala Leu His Leu Thr Trp Ser Tyr Ala Ser Phe Leu Thr Ala Thr Ala
450 455 460
Arg Arg Ala Gly Ile Val Pro Pro Ser Trp Ala Asn Ser Ser Ala Ser
465 470 475 480
Thr Ile Pro Ser Thr Cys Ser Gly Ala Ser Val Val Gly Ser Tyr Ser
485 490 495
Arg Pro Thr Ala Thr Ser Phe Pro Pro Ser Gln Thr Pro Lys Pro Gly
500 505 510
Val Pro Ser Gly Thr Pro Tyr Thr Pro Leu Pro Cys Ala Thr Pro Thr
515 520 525
Ser Val Ala Val Thr Phe His Glu Leu Val Ser Thr Gln Phe Gly Gln
530 535 540
Thr Val Lys Val Ala Gly Asn Ala Ala Ala Leu Gly Asn Trp Ser Thr
545 550 555 560
Ser Ala Ala Val Ala Leu Asp Ala Val Asn Tyr Ala Asp Asn His Pro
565 570 575
Leu Trp Ile Gly Thr Val Asn Leu Glu Ala Gly Asp Val Val Glu Tyr
580 585 590
Lys Tyr Ile Asn Val Gly Gln Asp Gly Ser Val Thr Trp Glu Ser Asp
595 600 605
Pro Asn His Thr Tyr Thr Val Pro Ala Val Ala Cys Val Thr Gln Val
610 615 620
Val Lys Glu Asp Thr Trp Gln Ser
625 630
<210> 112
<211> 569
<212> PRT
<213> 茯苓（Wolfiporia cocos）
<400> 112
Met Arg Leu Ser Leu Ala Ser Val Phe Ala Leu Ala Gly Gly Ala Leu
1 5 10 15
Ala Gln Thr Thr Ser Val Thr Ser Tyr Ile Ala Ser Glu Ser Pro Ile
20 25 30
Ala Lys Ala Gly Val Leu Ala Asn Ile Gly Ala Asp Gly Ser Leu Ser
35 40 45
Ser Gly Ala Tyr Ser Gly Ile Val Ile Ala Ser Pro Ser Thr Val Asn
50 55 60
Pro Asn Tyr Leu Tyr Thr Trp Thr Arg Asp Ser Ser Leu Thr Phe Met
65 70 75 80
Glu Leu Ile Asn Gln Tyr Ile Tyr Gly Glu Asp Asp Thr Leu Arg Thr
85 90 95
Leu Ile Asp Glu Phe Val Ser Ala Glu Ala Thr Leu Gln Gln Val Thr
100 105 110
Asn Pro Ser Gly Thr Val Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe
115 120 125
Asn Ile Asn Glu Thr Ala Phe Thr Gly Pro Trp Gly Arg Pro Gln Arg
130 135 140
Asp Gly Pro Ala Leu Arg Ala Thr Ala Ile Met Ala Tyr Ala Thr Tyr
145 150 155 160
Leu Tyr Glu Asn Gly Asn Thr Ser Tyr Val Thr Asp Thr Leu Trp Pro
165 170 175
Ile Ile Glu Leu Asp Leu Gly Tyr Val Ala Glu Tyr Trp Asn Glu Ser
180 185 190
Thr Phe Asp Leu Trp Glu Glu Ile Asp Ser Ser Ser Phe Phe Thr Thr
195 200 205
Ala Val Gln His Arg Ala Leu Arg Ala Gly Val Thr Phe Ala Asn Leu
210 215 220
Ile Gly Glu Thr Ser Asp Val Ser Asn Tyr Gln Glu Asn Ala Asp Asp
225 230 235 240
Leu Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Thr Gly Ser Tyr Val
245 250 255
Thr Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu
260 265 270
Leu Ala Ser Ile His Thr Phe Asp Pro Asp Ala Gly Cys Asn Ala Thr
275 280 285
Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn His Lys Val Tyr
290 295 300
Val Asp Ser Phe Arg Ser Leu Tyr Ala Ile Asn Asp Asp Ile Ser Ser
305 310 315 320
Asp Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Tyr Tyr Asn
325 330 335
Gly Asn Pro Trp Tyr Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu Tyr
340 345 350
Asp Ser Leu Ile Val Trp Lys Ala Gln Gly Tyr Ile Glu Val Thr Ser
355 360 365
Leu Ser Leu Ala Phe Phe Gln Gln Phe Asp Ala Ser Val Ser Ala Gly
370 375 380
Thr Tyr Asp Ser Ser Ser Asp Thr Tyr Thr Thr Leu Leu Asp Ala Val
385 390 395 400
Gln Thr Tyr Ala Asp Gly Phe Val Leu Met Val Ala Gln Tyr Thr Pro
405 410 415
Ala Asn Gly Ser Leu Ser Glu Gln Tyr Ala Lys Ala Asp Gly Ser Pro
420 425 430
Thr Ser Ala Tyr Asp Leu Thr Trp Ser Phe Ala Ala Ala Leu Thr Ala
435 440 445
Phe Ala Ala Arg Asp Gly Lys Thr Tyr Gly Ser Trp Gly Ala Ala Asp
450 455 460
Leu Ser Ser Thr Cys Ser Gly Ser Thr Asp Thr Val Ala Val Thr Phe
465 470 475 480
Glu Val Gln Tyr Asp Thr Gln Tyr Gly Glu Asn Leu Tyr Ile Thr Gly
485 490 495
Ser Val Ser Gln Leu Glu Asp Trp Ser Ala Asp Asp Ala Leu Ile Met
500 505 510
Ser Ser Ala Asp Tyr Pro Thr Trp Ser Ile Thr Val Asp Leu Pro Pro
515 520 525
Ser Thr Leu Ile Gln Tyr Lys Tyr Leu Thr Lys Tyr Asn Gly Asp Val
530 535 540
Thr Trp Glu Asp Asp Pro Asn Asn Glu Ile Thr Thr Pro Ala Ser Gly
545 550 555 560
Ser Tyr Thr Gln Val Asp Ser Trp His
565
<210> 113
<211> 8
<212> PRT
<213> 毛霉菌目（Mucorales）
<220>
<221> 尚未归类的特征
<222> (2)..(2)
<223> N或S
<220>
<221> 尚未归类的特征
<222> (3)..(3)
<223> T、S、或R
<220>
<221> 尚未归类的特征
<222> (5)..(6)
<223> 任何氨基酸
<220>
<221> 尚未归类的特征
<222> (7)..(7)
<223> G或N
<220>
<221> 尚未归类的特征
<222> (8)..(8)
<223> D、N、或S
<400> 113
Tyr Xaa Xaa Thr Xaa Xaa Xaa Xaa
1 5
<210> 114
<211> 8
<212> PRT
<213> 毛霉菌目（Mucorales）
<220>
<221> 尚未归类的特征
<222> (5)..(5)
<223> 任何氨基酸
<400> 114
Tyr Asn Thr Thr Xaa Ala Gly Asp
1 5
<210> 115
<211> 9
<212> PRT
<213> 毛霉菌目（Mucorales）
<220>
<221> 尚未归类的特征
<222> (1)..(1)
<223> S或A
<220>
<221> 尚未归类的特征
<222> (2)..(2)
<223> T、N、或V
<220>
<221> 尚未归类的特征
<222> (3)..(4)
<223> L或I
<220>
<221> 尚未归类的特征
<222> (8)..(8)
<223> 任何氨基酸
<220>
<221> 尚未归类的特征
<222> (9)..(9)
<223> A或G
<400> 115
Xaa Xaa Xaa Xaa Ala Ala Asn Xaa Xaa
1 5
<210> 116
<211> 9
<212> PRT
<213> 毛霉菌目（Mucorales）
<220>
<221> 尚未归类的特征
<222> (8)..(8)
<223> 任何氨基酸
<400> 116
Ser Thr Leu Ile Ala Ala Asn Xaa Ala
1 5
<210> 117
<211> 7
<212> PRT
<213> 毛霉菌目（Mucorales）
<220>
<221> 尚未归类的特征
<222> (1)..(1)
<223> N或D
<220>
<221> 尚未归类的特征
<222> (3)..(3)
<223> 任何氨基酸
<220>
<221> 尚未归类的特征
<222> (6)..(6)
<223> S或G
<220>
<221> 尚未归类的特征
<222> (7)..(7)
<223> Q、K、或E
<400> 117
Xaa Gly Xaa Gly Asn Xaa Xaa
1 5
<210> 118
<211> 7
<212> PRT
<213> 毛霉菌目（Mucorales）
<400> 118
Asn Gly Asn Gly Asn Ser Gln
1 5
<210> 119
<211> 468
<212> PRT
<213> Saksenaea oblongispora
<400> 119
Met Pro Ser Trp Lys Thr Leu Ile Phe Leu Leu Gly Pro Ile Thr Ala
1 5 10 15
Ala Leu Ala Ala Pro Val Asn Lys Gln Ser Phe Pro Thr Gly Asn Pro
20 25 30
Asp Ile Ser Ser Trp Val Ser Lys Gln Glu Gly Ile Ser Phe Ser Glu
35 40 45
Met Leu Arg Asn Val Asn Pro Pro Gly Thr Ala Lys Gly Phe Val Ala
50 55 60
Ala Ser Leu Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg
65 70 75 80
Asp Ala Ala Leu Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr
85 90 95
Asn Ala Gly Asp Asn Asn Ile His Gly Ala Leu Gln Asp Tyr Val Thr
100 105 110
Phe Gln Ile Asn Thr Gln Thr Glu Ser Thr Pro Cys Asn Cys Leu Gly
115 120 125
Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly
130 135 140
Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Met Ile Leu
145 150 155 160
Ile Ala Asp Ser Phe Leu Ala Gln Thr Lys Asp Thr Ala Tyr Val Thr
165 170 175
Asn Thr Leu Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn
180 185 190
Thr Trp Ser Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val
195 200 205
His Phe Tyr Thr Leu Met Val Met Arg Arg Gly Leu Leu Asp Gly Ala
210 215 220
Asp Phe Ala Thr Arg Asn Gly Asp Ala Ser Lys Ala Ser Ser Tyr Ser
225 230 235 240
Asp Ala Ala Ser Lys Ile Lys Thr Arg Leu Asp Ser Phe Trp Val Ser
245 250 255
Asp Lys Asn Tyr Ile Thr Val Thr Gln Asp Tyr Lys Glu Gly Val Lys
260 265 270
Lys Asp Thr Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala
275 280 285
Gly Lys Asp Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys Ile Leu Ala
290 295 300
Thr Ala Val Ala Phe Glu Asn Ala Met Ala Lys Leu Tyr Pro Leu Asn
305 310 315 320
Gln Asn Leu Asp Ser His Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu
325 330 335
Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu
340 345 350
Ala Thr Thr Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp
355 360 365
Lys Asp Ser Gly Val Thr Val Thr Pro Val Ser Lys Asp Phe Phe Ala
370 375 380
Lys Phe Asp Ser Asn Ala Val Ala Gly Lys Lys Tyr Ser Pro Asn Ser
385 390 395 400
Glu Glu Phe Ala Thr Leu Thr Ala Asn Ile Ala Ala Ala Ala Asp Arg
405 410 415
Phe Met Ser Thr Val Lys Tyr His Gln Asn Pro Asn Gly Ser Leu Ser
420 425 430
Glu Gln Phe Asp Arg His Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu
435 440 445
Thr Trp Ser His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly
450 455 460
Thr Pro Ser Phe
465
<210> 120
<211> 463
<212> PRT
<213> Apophysomyces ossiformis
<400> 120
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp
20 25 30
Val Ser Ser Gln Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile
35 40 45
Asn Pro Pro Gly Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr
50 55 60
Ala Gly Pro Asp Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val
65 70 75 80
Ser Arg Val Val Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser
85 90 95
Thr Val Ala Gly Phe Leu Glu Asp Tyr Val His Phe Gln Ile Asn Thr
100 105 110
Gln Thr Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn
115 120 125
Pro Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp
130 135 140
Gly Pro Ala Glu Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe
145 150 155 160
Leu Thr Gln Thr Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro
165 170 175
Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro
180 185 190
Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu
195 200 205
Met Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg
210 215 220
Asn Gly Asp Thr Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln
225 230 235 240
Ile Gln Asn Lys Ile Asp Ser Phe Trp Ser Ser Asn Lys Asn Tyr Ile
245 250 255
Ile Val Thr Gln Asp Tyr Gln Asn Gly Val Gln Lys Pro Ser Gly Leu
260 265 270
Asp Ile Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly
275 280 285
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
290 295 300
Glu Asn Ala Met Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser
305 310 315 320
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
325 330 335
Asp Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe
340 345 350
Ser Glu Leu Tyr Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val
355 360 365
Thr Val Thr Ser Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn
370 375 380
Ala Ser Ala Gly Lys Lys Tyr Ser Pro Gly Ser Asn Glu Phe Ala Ser
385 390 395 400
Leu Ala Gln Asn Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val
405 410 415
Asn Tyr His Arg Asn Ser Asn Gly Ser Leu Ser Glu Glu Phe Asp Arg
420 425 430
Asn Thr Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala
435 440 445
Ala Phe Ile Thr Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser Phe
450 455 460
<210> 121
<211> 462
<212> PRT
<213> 雅致鳞质霉（Apophysomyces elegans）
<400> 121
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp
20 25 30
Val Ser Ser Gln Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile
35 40 45
Asn Pro Pro Gly Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr
50 55 60
Ala Gly Pro Asp Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val
65 70 75 80
Ser Arg Val Val Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser
85 90 95
Thr Val Ala Gly Phe Leu Gln Asp Tyr Val His Phe Gln Ile Asn Thr
100 105 110
Gln Thr Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn
115 120 125
Pro Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp
130 135 140
Gly Pro Ala Glu Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe
145 150 155 160
Leu Thr Gln Thr Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro
165 170 175
Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro
180 185 190
Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu
195 200 205
Met Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg
210 215 220
Asn Gly Asp Thr Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln
225 230 235 240
Ile Gln Asn Lys Ile Asp Ser Phe Trp Ser Ser Ser Lys Asn Tyr Ile
245 250 255
Met Val Thr Gln Asp Tyr Gln Asn Gly Ile Gln Lys Pro Ser Gly Leu
260 265 270
Asp Ile Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly
275 280 285
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
290 295 300
Glu Asn Ala Met Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser
305 310 315 320
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
325 330 335
Asp Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe
340 345 350
Ser Glu Leu Tyr Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val
355 360 365
Thr Val Thr Ser Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn
370 375 380
Ala Ser Ala Gly Lys Lys Tyr Ser Pro Gly Ser Asp Glu Phe Asn Ser
385 390 395 400
Leu Ala Gln Asn Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val
405 410 415
Asn Tyr His Arg Asn Ser Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg
420 425 430
Asn Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala
435 440 445
Ala Phe Ile Thr Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser
450 455 460
<210> 122
<211> 463
<212> PRT
<213> Apophysomyces variabilis
<400> 122
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp
20 25 30
Val Ser Ser Gln Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile
35 40 45
Asn Pro Pro Gly Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr
50 55 60
Ala Gly Pro Asp Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val
65 70 75 80
Ser Arg Val Val Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser
85 90 95
Thr Val Ala Gly Phe Leu Gln Asp Tyr Val His Phe Gln Ile Asn Thr
100 105 110
Gln Thr Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn
115 120 125
Pro Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp
130 135 140
Gly Pro Ala Glu Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe
145 150 155 160
Leu Ala Gln Thr Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro
165 170 175
Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro
180 185 190
Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu
195 200 205
Met Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg
210 215 220
Asn Gly Asp Thr Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln
225 230 235 240
Ile Gln Asn Lys Ile Asp Ser Phe Trp Ser Ser Ser Lys Asn Tyr Ile
245 250 255
Met Val Thr Gln Asp Tyr Gln Asn Gly Val Gln Lys Pro Ser Gly Leu
260 265 270
Asp Ile Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly
275 280 285
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
290 295 300
Glu Asn Ala Met Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser
305 310 315 320
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
325 330 335
Asp Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe
340 345 350
Ser Glu Leu Tyr Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val
355 360 365
Thr Val Thr Ser Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn
370 375 380
Ala Ser Ala Gly Lys Lys Tyr Ser Pro Gly Ser Asp Glu Phe Asn Ser
385 390 395 400
Leu Ala Gln Asn Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val
405 410 415
Asn Tyr His Arg Asn Ser Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg
420 425 430
Asn Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala
435 440 445
Ala Phe Ile Thr Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser Phe
450 455 460
<210> 123
<211> 463
<212> PRT
<213> Apophysomyces trapeziformis
<400> 123
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp
20 25 30
Val Ser Ser Gln Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile
35 40 45
Asn Pro Pro Gly Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr
50 55 60
Ala Gly Pro Asp Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val
65 70 75 80
Ser Arg Val Val Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser
85 90 95
Thr Val Ala Gly Phe Leu Gln Asp Tyr Val His Phe Gln Ile Asn Thr
100 105 110
Gln Thr Glu Ser Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn
115 120 125
Pro Asp Gly Ser Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp
130 135 140
Gly Pro Ala Glu Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe
145 150 155 160
Leu Thr Gln Thr Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro
165 170 175
Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro
180 185 190
Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu
195 200 205
Met Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg
210 215 220
Asn Gly Asp Thr Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln
225 230 235 240
Ile Gln Asn Lys Ile Asp Ser Phe Trp Ser Ser Ser Lys Asn Tyr Ile
245 250 255
Ile Val Thr Gln Asp Tyr Gln Asn Gly Val Gln Lys Pro Ser Gly Leu
260 265 270
Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly
275 280 285
Phe Tyr Thr Pro Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile
290 295 300
Glu Asn Ala Met Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser
305 310 315 320
Tyr Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly
325 330 335
Asp Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe
340 345 350
Ser Glu Leu Tyr Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val
355 360 365
Thr Val Thr Ser Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn
370 375 380
Ala Ser Ala Gly Lys Lys Tyr Ser Pro Gly Ser Asn Glu Tyr Ala Ser
385 390 395 400
Leu Ala Gln Asn Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val
405 410 415
Asn Tyr His Arg Asn Pro Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg
420 425 430
Asn Thr Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala
435 440 445
Ala Phe Ile Thr Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser Phe
450 455 460
<210> 124
<211> 1404
<212> DNA
<213> 人工序列
<220>
<223> 密码子修饰的
<400> 124
atgccttctt ggaagaccct gattttcctc ctgggcccta ttaccgccgc tctggctgct 60
cctgttaaca agcagtcttt ccctaccggc aaccccgaca tcagctcttg ggtttctaag 120
caggagggca ttagcttcag cgagatgctc cgaaacgtca accctcccgg caccgctaag 180
ggcttcgttg ctgctagcct cagcaccgcc ggccccgact acttctacac ctggacccga 240
gacgccgctc tcgtctcccg cgtcatcgct tacaagtaca acaccaccaa cgctggcgac 300
aacaacattc acggcgctct gcaggactac gtcaccttcc agattaacac ccagaccgag 360
tccacccctt gcaactgcct gggcgagcct aagttcaacc ccgacggcag ctctttcacc 420
ggcccttggg gccgacctca gaacgacggc cctgctgagc gcgctagcag catgattctc 480
attgctgaca gcttcctcgc ccagaccaag gacaccgctt acgttaccaa caccctgaag 540
cctgccattt acaaggacct ggactacgtt gttaacacct ggtctaaccc ttgcttcgac 600
ctgtgggagg aggttaacgg cgtccacttc tacaccctca tggttatgcg acgaggcctc 660
ctcgacggcg ctgacttcgc tacccgaaac ggcgacgcct ctaaggctag ctcttactct 720
gacgctgcta gcaagatcaa gacccgactg gactctttct gggtcagcga caagaactac 780
attaccgtta cccaggacta caaggagggc gtcaagaagg acaccggcct cgacgttagc 840
accctcattg ctgccaacgt tgccggcaag gacgacggct tctacacccc cggcagcgac 900
aagattctgg ctaccgctgt tgccttcgag aacgccatgg ctaagctgta ccctctgaac 960
cagaacctcg acagccacct gggcaactct attggccgat accctgagga cacctacaac 1020
ggcaacggca actcccaggg caacccttgg ttcctcgcca ccaccgcttt caccgagctg 1080
tactaccgag ccatcttgga atggaaggac agcggcgtta ccgtcacccc cgtttccaag 1140
gacttcttcg ccaagttcga ctccaacgcc gttgccggca agaagtactc tcctaactct 1200
gaggagttcg ccaccctgac cgctaacatc gctgctgccg ctgaccgatt catgtctacc 1260
gttaagtacc accagaaccc taacggctct ctgtccgagc agttcgaccg ccacagcggc 1320
tacatgaccg gcgcccgaga cctgacctgg tctcacgctg ctttcattac cgccgcccag 1380
gctcgcgctg gcacccctag cttc 1404
<210> 125
<211> 19
<212> PRT
<213> 人工序列
<220>
<223> 信号肽
<400> 125
Met Pro Ser Trp Lys Thr Leu Ile Phe Leu Leu Gly Pro Ile Thr Ala
1 5 10 15
Ala Leu Ala
<210> 126
<211> 449
<212> PRT
<213> Saksenaea oblongispora
<400> 126
Ala Pro Val Asn Lys Gln Ser Phe Pro Thr Gly Asn Pro Asp Ile Ser
1 5 10 15
Ser Trp Val Ser Lys Gln Glu Gly Ile Ser Phe Ser Glu Met Leu Arg
20 25 30
Asn Val Asn Pro Pro Gly Thr Ala Lys Gly Phe Val Ala Ala Ser Leu
35 40 45
Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ala Ala
50 55 60
Leu Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr Asn Ala Gly
65 70 75 80
Asp Asn Asn Ile His Gly Ala Leu Gln Asp Tyr Val Thr Phe Gln Ile
85 90 95
Asn Thr Gln Thr Glu Ser Thr Pro Cys Asn Cys Leu Gly Glu Pro Lys
100 105 110
Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln
115 120 125
Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Met Ile Leu Ile Ala Asp
130 135 140
Ser Phe Leu Ala Gln Thr Lys Asp Thr Ala Tyr Val Thr Asn Thr Leu
145 150 155 160
Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asn Thr Trp Ser
165 170 175
Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Val His Phe Tyr
180 185 190
Thr Leu Met Val Met Arg Arg Gly Leu Leu Asp Gly Ala Asp Phe Ala
195 200 205
Thr Arg Asn Gly Asp Ala Ser Lys Ala Ser Ser Tyr Ser Asp Ala Ala
210 215 220
Ser Lys Ile Lys Thr Arg Leu Asp Ser Phe Trp Val Ser Asp Lys Asn
225 230 235 240
Tyr Ile Thr Val Thr Gln Asp Tyr Lys Glu Gly Val Lys Lys Asp Thr
245 250 255
Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Lys Asp
260 265 270
Asp Gly Phe Tyr Thr Pro Gly Ser Asp Lys Ile Leu Ala Thr Ala Val
275 280 285
Ala Phe Glu Asn Ala Met Ala Lys Leu Tyr Pro Leu Asn Gln Asn Leu
290 295 300
Asp Ser His Leu Gly Asn Ser Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
305 310 315 320
Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr
325 330 335
Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Lys Asp Ser
340 345 350
Gly Val Thr Val Thr Pro Val Ser Lys Asp Phe Phe Ala Lys Phe Asp
355 360 365
Ser Asn Ala Val Ala Gly Lys Lys Tyr Ser Pro Asn Ser Glu Glu Phe
370 375 380
Ala Thr Leu Thr Ala Asn Ile Ala Ala Ala Ala Asp Arg Phe Met Ser
385 390 395 400
Thr Val Lys Tyr His Gln Asn Pro Asn Gly Ser Leu Ser Glu Gln Phe
405 410 415
Asp Arg His Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
420 425 430
His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly Thr Pro Ser
435 440 445
Phe
<210> 127
<211> 1389
<212> DNA
<213> 人工序列
<220>
<223> 密码子修饰的
<400> 127
atgatgatgt cttggaagtc tctgctcctc ctgctgggcc ctatcgccgc tgctaccgct 60
gccatcccta ccggcaacag cagcctgaac gcttgggtta gctcccagga ggacatctct 120
ttctccgtca tgctgggcaa cattaaccct cccggcaccg tcaagggctt cgttgccgct 180
agcctgtcca ccgctggccc cgactacttc tactcttggg tccgagacag cgccctcgtt 240
tcccgcgtcg tcacccacaa gtacaacacc accgagaccg gcaactctac cgttgccggc 300
ttcttggagg actacgttca cttccagatt aacacccaga ccgagtctac cgtttgcaac 360
tgcctgggcg agcctaagtt caaccccgac ggcagctctt acaccggccc ttggggccgc 420
cctcagaacg acggccccgc tgagcgagct agcaccatga ttctgattgc cgactctttc 480
ctgacccaga ccaagaacac ctcttacgtt gacaacaccc tgaagcccgc catttacaag 540
gacctggact acgttgttaa cacctggtct aacccttgct tcgacctgtg ggaggaggtt 600
aacggcattc acttctacac cctcatggtt atgcgacgag gcctcctcga cggcgccaac 660
ttcgccaccc gaaacggcga cacctctaag gctagctctt acagcagcac cgctagccag 720
attcagaaca agattgactc cttctggagc tccaacaaga actacattat tgttacccag 780
gactaccaga acggcgtcca gaagcctagc ggcctcgaca tcagcaccct cattgctgcc 840
aacgtcgctg gcatgaacga cggcttctac acccccggct ctgacaagat gctcgccacc 900
gccgttgcta ttgagaacgc catggctaac ctgtaccccc tcaacaagaa cctgccttct 960
tacctgggca acgctattgg ccgataccct gaggacacct acaacggcga cggcaactcc 1020
cagggcaacc cttggttcct cgccaccacc gctttcagcg agctgtacta ccgagctctg 1080
ctggagtggc aggagaccgg cgttaccgtt accagcattt ctaaggactt cttcagcaag 1140
ttcgacccta acgctagcgc tggcaagaag tactctcccg gctctaacga gttcgcttct 1200
ctggctcaga acattgctag cgctgctgac cgattcctgt ccaccgtcaa ctaccaccga 1260
aactccaacg gcagcctgtc tgaggagttc gaccgaaaca ccggctacat gaccggcgcc 1320
cgagacctga cctggtctca cgccgctttc attaccgctg gcctcgctcg cgctggcacc 1380
cctagcttc 1389
<210> 128
<211> 20
<212> PRT
<213> 人工序列
<220>
<223> 信号肽
<400> 128
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala
20
<210> 129
<211> 443
<212> PRT
<213> Apophysomyces ossiformis
<400> 129
Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp Val Ser Ser Gln
1 5 10 15
Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile Asn Pro Pro Gly
20 25 30
Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr Ala Gly Pro Asp
35 40 45
Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val Ser Arg Val Val
50 55 60
Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser Thr Val Ala Gly
65 70 75 80
Phe Leu Glu Asp Tyr Val His Phe Gln Ile Asn Thr Gln Thr Glu Ser
85 90 95
Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser
100 105 110
Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu
115 120 125
Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe Leu Thr Gln Thr
130 135 140
Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro Ala Ile Tyr Lys
145 150 155 160
Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro Cys Phe Asp Leu
165 170 175
Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu Met Val Met Arg
180 185 190
Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg Asn Gly Asp Thr
195 200 205
Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln Ile Gln Asn Lys
210 215 220
Ile Asp Ser Phe Trp Ser Ser Asn Lys Asn Tyr Ile Ile Val Thr Gln
225 230 235 240
Asp Tyr Gln Asn Gly Val Gln Lys Pro Ser Gly Leu Asp Ile Ser Thr
245 250 255
Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly Phe Tyr Thr Pro
260 265 270
Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile Glu Asn Ala Met
275 280 285
Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser Tyr Leu Gly Asn
290 295 300
Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asp Gly Asn Ser
305 310 315 320
Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe Ser Glu Leu Tyr
325 330 335
Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val Thr Val Thr Ser
340 345 350
Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn Ala Ser Ala Gly
355 360 365
Lys Lys Tyr Ser Pro Gly Ser Asn Glu Phe Ala Ser Leu Ala Gln Asn
370 375 380
Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val Asn Tyr His Arg
385 390 395 400
Asn Ser Asn Gly Ser Leu Ser Glu Glu Phe Asp Arg Asn Thr Gly Tyr
405 410 415
Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala Phe Ile Thr
420 425 430
Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser Phe
435 440
<210> 130
<211> 1386
<212> DNA
<213> 人工序列
<220>
<223> 密码子优化的
<400> 130
atgatgatgt cttggaagtc tctgctcctg ctcctgggcc ctattgctgc cgccaccgct 60
gccatcccta ccggcaactc tagcctcaac gcttgggtta gcagccagga ggacatttct 120
ttcagcgtca tgctgggcaa cattaaccct cccggcaccg tcaagggctt cgttgctgct 180
agcctgtcca ccgctggccc cgactacttc tactcttggg ttcgagacag cgctctcgtt 240
tctcgcgtcg ttacccacaa gtacaacacc accgagaccg gcaacagcac cgttgctggc 300
ttcctgcagg actacgttca cttccagatt aacacccaga ccgagtccac cgtttgcaac 360
tgcctgggcg agcctaagtt caaccccgac ggcagctctt acaccggccc ttggggccgc 420
cctcagaacg acggccccgc tgagcgagct agcaccatga ttctgattgc tgactccttc 480
ctgacccaga ccaagaacac ctcttacgtt gacaacaccc tgaagcccgc tatttacaag 540
gacctggact acgtcgtcaa cacctggtct aacccttgct tcgacctgtg ggaggaggtt 600
aacggcattc acttctacac cctcatggtt atgcgacgag gcctcctgga cggcgccaac 660
ttcgccaccc gaaacggcga caccagcaag gctagctctt acagcagcac cgcttctcag 720
atccagaaca agattgactc tttctggtct agctctaaga actacatcat ggttacccag 780
gactaccaga acggcattca gaagcctagc ggcctcgaca ttagcaccct catcgccgct 840
aacgtcgctg gcatgaacga cggcttctac acccccggct ccgacaagat gctcgctacc 900
gccgttgcta ttgagaacgc catggctaac ctgtaccctc tgaacaagaa cctgccttct 960
tacctgggca acgccattgg ccgatacccc gaggacacct acaacggcga cggcaactct 1020
cagggcaacc cttggttcct cgccaccacc gctttcagcg agctgtacta ccgcgctctc 1080
ctggagtggc aggagaccgg cgttaccgtc accagcattt ccaaggactt cttctctaag 1140
ttcgacccta acgctagcgc tggcaagaag tactcccccg gctctgacga gttcaactcc 1200
ctcgcccaga acattgccag cgctgccgac cgattcctgt ctaccgttaa ctaccaccga 1260
aactccaacg gctctctgtc tgaggagtac gaccgaaaca gcggctacat gaccggcgcc 1320
cgagacctga cctggtccca cgccgctttc attaccgccg gcctcgcgag agctggcacc 1380
cctagc 1386
<210> 131
<211> 20
<212> PRT
<213> 人工序列
<220>
<223> 信号肽
<400> 131
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala
20
<210> 132
<211> 442
<212> PRT
<213> 雅致鳞质霉（Apophysomyces elegans）
<400> 132
Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp Val Ser Ser Gln
1 5 10 15
Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile Asn Pro Pro Gly
20 25 30
Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr Ala Gly Pro Asp
35 40 45
Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val Ser Arg Val Val
50 55 60
Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser Thr Val Ala Gly
65 70 75 80
Phe Leu Gln Asp Tyr Val His Phe Gln Ile Asn Thr Gln Thr Glu Ser
85 90 95
Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser
100 105 110
Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu
115 120 125
Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe Leu Thr Gln Thr
130 135 140
Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro Ala Ile Tyr Lys
145 150 155 160
Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro Cys Phe Asp Leu
165 170 175
Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu Met Val Met Arg
180 185 190
Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg Asn Gly Asp Thr
195 200 205
Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln Ile Gln Asn Lys
210 215 220
Ile Asp Ser Phe Trp Ser Ser Ser Lys Asn Tyr Ile Met Val Thr Gln
225 230 235 240
Asp Tyr Gln Asn Gly Ile Gln Lys Pro Ser Gly Leu Asp Ile Ser Thr
245 250 255
Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly Phe Tyr Thr Pro
260 265 270
Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile Glu Asn Ala Met
275 280 285
Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser Tyr Leu Gly Asn
290 295 300
Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asp Gly Asn Ser
305 310 315 320
Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe Ser Glu Leu Tyr
325 330 335
Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val Thr Val Thr Ser
340 345 350
Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn Ala Ser Ala Gly
355 360 365
Lys Lys Tyr Ser Pro Gly Ser Asp Glu Phe Asn Ser Leu Ala Gln Asn
370 375 380
Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val Asn Tyr His Arg
385 390 395 400
Asn Ser Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Asn Ser Gly Tyr
405 410 415
Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala Phe Ile Thr
420 425 430
Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser
435 440
<210> 133
<211> 1389
<212> DNA
<213> 人工序列
<220>
<223> 密码子修饰的
<400> 133
atgatgatgt cttggaagtc tctgctcctc ctgctgggcc ctattgccgc tgccaccgct 60
gccattccta ccggcaacag cagcctcaac gcttgggtta gctcccagga ggacatttct 120
ttcagcgtca tgctgggcaa cattaaccct cccggcaccg ttaagggctt cgttgccgct 180
agcctgtcta ccgccggccc cgactacttc tactcttggg tccgagacag cgccctcgtt 240
tcccgcgtcg tcacccacaa gtacaacacc accgagaccg gcaacagcac cgttgctggc 300
ttcctgcagg actacgtcca cttccagatt aacacccaga ccgagtccac cgtttgcaac 360
tgcctgggcg agcctaagtt caaccccgac ggcagctctt acaccggccc ttggggccga 420
cctcagaacg acggccccgc tgagcgcgct agcaccatga ttctgattgc tgactccttc 480
ctcgctcaga ccaagaacac ctcttacgtt gacaacaccc tgaagcctgc catttacaag 540
gacctggact acgtcgttaa cacctggtct aacccttgct tcgacctgtg ggaggaggtt 600
aacggcattc acttctacac cctcatggtt atgcgacgag gcctcctgga cggcgccaac 660
ttcgccaccc gaaacggcga cacctccaag gctagctctt acagcagcac cgctagccag 720
atccagaaca agattgactc tttctggtct agctccaaga actacatcat ggttacccag 780
gactaccaga acggcgttca gaagcctagc ggcctcgaca ttagcaccct catcgccgct 840
aacgttgctg gcatgaacga cggcttctac acccctggct ctgacaagat gctcgccacc 900
gccgttgcta ttgagaacgc tatggctaac ctgtaccctc tgaacaagaa cctgccttct 960
tacctgggca acgccattgg ccgatacccc gaggacacct acaacggcga cggcaactcc 1020
cagggcaacc cttggttcct cgctaccacc gctttcagcg agctgtacta ccgagctctg 1080
ttagagtggc aggagaccgg cgtcaccgtt accagcatct ctaaggactt cttctccaag 1140
ttcgacccta acgctagcgc cggcaagaag tactcccccg gcagcgacga gttcaactct 1200
ctcgcccaga acatcgccag cgccgctgac cgattcctgt ctaccgtcaa ctaccaccga 1260
aactctaacg gctctctgtc tgaggagtac gaccgaaact ccggctacat gaccggcgcg 1320
agagacctga cctggtctca cgctgctttc attaccgctg gcctcgcccg agctggcacc 1380
ccttctttc 1389
<210> 134
<211> 20
<212> PRT
<213> 人工序列
<220>
<223> 信号肽
<400> 134
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala
20
<210> 135
<211> 443
<212> PRT
<213> Apophysomyces variabilis
<400> 135
Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp Val Ser Ser Gln
1 5 10 15
Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile Asn Pro Pro Gly
20 25 30
Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr Ala Gly Pro Asp
35 40 45
Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val Ser Arg Val Val
50 55 60
Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser Thr Val Ala Gly
65 70 75 80
Phe Leu Gln Asp Tyr Val His Phe Gln Ile Asn Thr Gln Thr Glu Ser
85 90 95
Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser
100 105 110
Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu
115 120 125
Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe Leu Ala Gln Thr
130 135 140
Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro Ala Ile Tyr Lys
145 150 155 160
Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro Cys Phe Asp Leu
165 170 175
Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu Met Val Met Arg
180 185 190
Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg Asn Gly Asp Thr
195 200 205
Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln Ile Gln Asn Lys
210 215 220
Ile Asp Ser Phe Trp Ser Ser Ser Lys Asn Tyr Ile Met Val Thr Gln
225 230 235 240
Asp Tyr Gln Asn Gly Val Gln Lys Pro Ser Gly Leu Asp Ile Ser Thr
245 250 255
Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly Phe Tyr Thr Pro
260 265 270
Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile Glu Asn Ala Met
275 280 285
Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser Tyr Leu Gly Asn
290 295 300
Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asp Gly Asn Ser
305 310 315 320
Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe Ser Glu Leu Tyr
325 330 335
Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val Thr Val Thr Ser
340 345 350
Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn Ala Ser Ala Gly
355 360 365
Lys Lys Tyr Ser Pro Gly Ser Asp Glu Phe Asn Ser Leu Ala Gln Asn
370 375 380
Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val Asn Tyr His Arg
385 390 395 400
Asn Ser Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Asn Ser Gly Tyr
405 410 415
Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala Phe Ile Thr
420 425 430
Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser Phe
435 440
<210> 136
<211> 1389
<212> DNA
<213> 人工序列
<220>
<223> 密码子修饰的
<400> 136
atgatgatgt cttggaagtc tctgctcctc ctgctgggcc ctatcgccgc tgccaccgct 60
gccatcccta ccggcaacag cagcctcaac gcttgggtta gctctcagga ggacatctct 120
ttcagcgtca tgctgggcaa catcaaccct cccggcaccg tcaagggctt cgttgccgct 180
agcctgtcta ccgctggccc cgactacttc tactcttggg tccgagacag cgctctcgtt 240
tctcgcgttg ttacccacaa gtacaacacc accgagaccg gcaacagcac cgttgctggc 300
ttcctgcagg actacgttca cttccagatt aacacccaga ccgagtccac cgtttgcaac 360
tgcctgggcg agcctaagtt caaccccgac ggcagctctt acaccggccc ttggggccgc 420
cctcagaacg acggccccgc tgagcgcgct agcaccatga ttctgatcgc tgactccttc 480
ctgacccaga ccaagaacac ctcttacgtt gacaacaccc tgaagcccgc tatttacaag 540
gacctcgact acgtcgttaa cacctggtct aacccttgct tcgacctgtg ggaggaggtt 600
aacggcattc acttctacac cctcatggtt atgcgacgag gcctcctgga cggcgccaac 660
ttcgccaccc gaaacggcga caccagcaag gctagctctt actccagcac cgcttcccag 720
attcagaaca agattgactc tttctggagc agctccaaga actacattat tgttacccag 780
gactaccaga acggcgtcca gaagcctagc ggcctcgacg tcagcaccct cattgctgcc 840
aacgtcgctg gcatgaacga cggcttctac acccccggca gcgacaagat gctcgccacc 900
gccgttgcca ttgagaacgc catggctaac ctgtaccctc tgaacaagaa cctgccttct 960
tacctgggca acgctattgg ccgatacccc gaggacacct acaacggcga cggcaactcc 1020
cagggcaacc cttggttcct cgccaccacc gctttcagcg agctgtacta ccgagctctg 1080
cttgaatggc aggagaccgg cgtcaccgtt accagcattt ctaaggactt cttctctaag 1140
ttcgacccta acgctagcgc cggcaagaag tactcccccg gctccaacga gtacgctagc 1200
ctcgcccaga acattgctag cgccgctgac cgattcctgt ctaccgttaa ctaccaccga 1260
aaccctaacg gctctctgtc tgaggagtac gaccgaaaca ccggctacat gaccggcgcc 1320
cgagacctga cctggtctca cgctgctttc attaccgccg gcctggccag ggctggcacc 1380
ccttctttc 1389
<210> 137
<211> 20
<212> PRT
<213> 人工序列
<220>
<223> 信号肽
<400> 137
Met Met Met Ser Trp Lys Ser Leu Leu Leu Leu Leu Gly Pro Ile Ala
1 5 10 15
Ala Ala Thr Ala
20
<210> 138
<211> 443
<212> PRT
<213> Apophysomyces trapeziformis
<400> 138
Ala Ile Pro Thr Gly Asn Ser Ser Leu Asn Ala Trp Val Ser Ser Gln
1 5 10 15
Glu Asp Ile Ser Phe Ser Val Met Leu Gly Asn Ile Asn Pro Pro Gly
20 25 30
Thr Val Lys Gly Phe Val Ala Ala Ser Leu Ser Thr Ala Gly Pro Asp
35 40 45
Tyr Phe Tyr Ser Trp Val Arg Asp Ser Ala Leu Val Ser Arg Val Val
50 55 60
Thr His Lys Tyr Asn Thr Thr Glu Thr Gly Asn Ser Thr Val Ala Gly
65 70 75 80
Phe Leu Gln Asp Tyr Val His Phe Gln Ile Asn Thr Gln Thr Glu Ser
85 90 95
Thr Val Cys Asn Cys Leu Gly Glu Pro Lys Phe Asn Pro Asp Gly Ser
100 105 110
Ser Tyr Thr Gly Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Glu
115 120 125
Arg Ala Ser Thr Met Ile Leu Ile Ala Asp Ser Phe Leu Thr Gln Thr
130 135 140
Lys Asn Thr Ser Tyr Val Asp Asn Thr Leu Lys Pro Ala Ile Tyr Lys
145 150 155 160
Asp Leu Asp Tyr Val Val Asn Thr Trp Ser Asn Pro Cys Phe Asp Leu
165 170 175
Trp Glu Glu Val Asn Gly Ile His Phe Tyr Thr Leu Met Val Met Arg
180 185 190
Arg Gly Leu Leu Asp Gly Ala Asn Phe Ala Thr Arg Asn Gly Asp Thr
195 200 205
Ser Lys Ala Ser Ser Tyr Ser Ser Thr Ala Ser Gln Ile Gln Asn Lys
210 215 220
Ile Asp Ser Phe Trp Ser Ser Ser Lys Asn Tyr Ile Ile Val Thr Gln
225 230 235 240
Asp Tyr Gln Asn Gly Val Gln Lys Pro Ser Gly Leu Asp Val Ser Thr
245 250 255
Leu Ile Ala Ala Asn Val Ala Gly Met Asn Asp Gly Phe Tyr Thr Pro
260 265 270
Gly Ser Asp Lys Met Leu Ala Thr Ala Val Ala Ile Glu Asn Ala Met
275 280 285
Ala Asn Leu Tyr Pro Leu Asn Lys Asn Leu Pro Ser Tyr Leu Gly Asn
290 295 300
Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr Asn Gly Asp Gly Asn Ser
305 310 315 320
Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr Ala Phe Ser Glu Leu Tyr
325 330 335
Tyr Arg Ala Leu Leu Glu Trp Gln Glu Thr Gly Val Thr Val Thr Ser
340 345 350
Ile Ser Lys Asp Phe Phe Ser Lys Phe Asp Pro Asn Ala Ser Ala Gly
355 360 365
Lys Lys Tyr Ser Pro Gly Ser Asn Glu Tyr Ala Ser Leu Ala Gln Asn
370 375 380
Ile Ala Ser Ala Ala Asp Arg Phe Leu Ser Thr Val Asn Tyr His Arg
385 390 395 400
Asn Pro Asn Gly Ser Leu Ser Glu Glu Tyr Asp Arg Asn Thr Gly Tyr
405 410 415
Met Thr Gly Ala Arg Asp Leu Thr Trp Ser His Ala Ala Phe Ile Thr
420 425 430
Ala Gly Leu Ala Arg Ala Gly Thr Pro Ser Phe
435 440
<210> 139
<211> 1404
<212> DNA
<213> 人工序列
<220>
<223> 人工序列
<400> 139
atgccttctt ggaaaaccct gttcctcctc ctgggcccta tcgccaccgc tgccgctgct 60
cccgttgaca agcagtctct gcctaccggc aacagcacca ttagctcttg ggtcagcaag 120
caggaggaca tcagcttcag cgagatgctc cgaaacgtca accctgaggg caccgccaag 180
ggcttcgttg ccgcgtcgct cagcaccgct ggccccgact acttctacac ctggacccga 240
gacgccgctc tcgcctcccg agttattgct tacaagtaca acaccaccaa cgctggcgac 300
tctaagatcc acggcgttct ggacgactac gttaacttcc agattaacac ccagtctgag 360
cccacccctt gcaactgcct gggcgagcct aagttcaacc ctgacggcag ctctttcacc 420
ggcccttggg gccgccccca gaacgacggc cccgctgagc gagcctccag tttcatgctg 480
attgccgact ctttcctcag ccagaccaag aacgcctctt acttcaccaa caccctcaag 540
cctgccattt acaaggacct cgactacgtc gttgacacct ggtctaaccc ttgcttcgac 600
ctgtgggagg aggttaacgg catccacttc tacaccctca tggttatgcg acgatctctc 660
ctcgacggcg ctaacttcgc cacccgaaac ggcgacaact ccaaggcctc aacctactcc 720
ggcgtcgccg ccaagattca ggctcgactg aactctttct gggacgccgg caagaactac 780
attaccgtta cccaggacta caagaacggc gttgagaagc ctagcggcct cgacgttagc 840
accctcattg ctgctaacgt cgctggcatg ggcgacggct tctacacccc cggcagcgag 900
cgcatcctcg ctaccgctct ggctttcgag aagagcatgg caagtctgta ccctctgaac 960
aacaacctgc ctagccacct gggcaacgcc attggccgat accccgagga cacctacaac 1020
ggcaacggca actcccaggg caacccttgg ttcctcgcta ccaccgcttt caccgagctg 1080
tactaccgcg ccattctgga gtggaagaac accggcgtta ccgtcacccc tatttctaag 1140
gacttcttcg tccgattcga cagcagcgct gctcctggca agaagtacaa ccccggctcc 1200
caggagttcg ctaccctgac ccagtctatt gctgccgccg ctgaccgatt catgtccacc 1260
gtccagtacc accagaaccc taacggctct ctgtctgagg agttcgaccg atctagcggc 1320
tacatgaccg gcgcccgaga cctgacctgg agtcacgctg ctttcattac cgctgctcag 1380
gcccgagctg gctctccttc tttc 1404
<210> 140
<211> 449
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 140
Ala Pro Val Asp Lys Gln Ser Leu Pro Thr Gly Asn Ser Thr Ile Ser
1 5 10 15
Ser Trp Val Ser Lys Gln Glu Asp Ile Ser Phe Ser Glu Met Leu Arg
20 25 30
Asn Val Asn Pro Glu Gly Thr Ala Lys Gly Phe Val Ala Ala Ser Leu
35 40 45
Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ala Ala
50 55 60
Leu Ala Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr Asn Ala Gly
65 70 75 80
Asp Ser Lys Ile His Gly Val Leu Asp Asp Tyr Val Asn Phe Gln Ile
85 90 95
Asn Thr Gln Ser Glu Pro Thr Pro Cys Asn Cys Leu Gly Glu Pro Lys
100 105 110
Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln
115 120 125
Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Phe Met Leu Ile Ala Asp
130 135 140
Ser Phe Leu Ser Gln Thr Lys Asn Ala Ser Tyr Phe Thr Asn Thr Leu
145 150 155 160
Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asp Thr Trp Ser
165 170 175
Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr
180 185 190
Thr Leu Met Val Met Arg Arg Ser Leu Leu Asp Gly Ala Asn Phe Ala
195 200 205
Thr Arg Asn Gly Asp Asn Ser Lys Ala Ser Thr Tyr Ser Gly Val Ala
210 215 220
Ala Lys Ile Gln Ala Arg Leu Asn Ser Phe Trp Asp Ala Gly Lys Asn
225 230 235 240
Tyr Ile Thr Val Thr Gln Asp Tyr Lys Asn Gly Val Glu Lys Pro Ser
245 250 255
Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Gly
260 265 270
Asp Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala Thr Ala Leu
275 280 285
Ala Phe Glu Lys Ser Met Ala Ser Leu Tyr Pro Leu Asn Asn Asn Leu
290 295 300
Pro Ser His Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
305 310 315 320
Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr
325 330 335
Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Lys Asn Thr
340 345 350
Gly Val Thr Val Thr Pro Ile Ser Lys Asp Phe Phe Val Arg Phe Asp
355 360 365
Ser Ser Ala Ala Pro Gly Lys Lys Tyr Asn Pro Gly Ser Gln Glu Phe
370 375 380
Ala Thr Leu Thr Gln Ser Ile Ala Ala Ala Ala Asp Arg Phe Met Ser
385 390 395 400
Thr Val Gln Tyr His Gln Asn Pro Asn Gly Ser Leu Ser Glu Glu Phe
405 410 415
Asp Arg Ser Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
420 425 430
His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly Ser Pro Ser
435 440 445
Phe
<210> 141
<211> 449
<212> PRT
<213> 人工序列
<220>
<223> 人工序列
<400> 141
Ala Pro Val Asp Lys Gln Ser Leu Pro Thr Gly Asn Ser Thr Ile Ser
1 5 10 15
Ser Trp Val Ser Lys Gln Glu Asp Ile Ser Phe Ser Glu Met Leu Arg
20 25 30
Asn Val Asn Pro Glu Gly Thr Ala Lys Gly Phe Val Ala Ala Ser Leu
35 40 45
Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ala Ala
50 55 60
Leu Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr Asn Ala Gly
65 70 75 80
Asp Ser Lys Ile His Gly Val Leu Asp Asp Tyr Val Asn Phe Gln Ile
85 90 95
Asn Thr Gln Ser Glu Pro Thr Pro Cys Asn Cys Leu Gly Glu Pro Lys
100 105 110
Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly Arg Pro Gln
115 120 125
Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Phe Met Leu Ile Ala Asp
130 135 140
Ser Phe Leu Ser Gln Thr Lys Asn Ala Ser Tyr Phe Thr Asn Thr Leu
145 150 155 160
Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asp Thr Trp Ser
165 170 175
Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile His Phe Tyr
180 185 190
Thr Leu Met Val Met Arg Arg Ser Leu Leu Asp Gly Ala Asn Phe Ala
195 200 205
Thr Arg Asn Gly Asp Asn Ser Lys Ala Ser Thr Tyr Ser Gly Val Ala
210 215 220
Ala Lys Ile Gln Ala Arg Leu Asn Ser Phe Trp Asp Ala Gly Lys Asn
225 230 235 240
Tyr Ile Thr Val Thr Gln Asp Tyr Lys Asn Gly Val Glu Lys Pro Ser
245 250 255
Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala Gly Met Gly
260 265 270
Asp Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala Thr Ala Leu
275 280 285
Ala Phe Glu Lys Ser Met Ala Ser Leu Tyr Pro Leu Asn Asn Asn Leu
290 295 300
Pro Ser His Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu Asp Thr Tyr
305 310 315 320
Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu Ala Thr Thr
325 330 335
Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp Lys Asn Thr
340 345 350
Gly Val Thr Val Thr Pro Ile Ser Lys Asp Phe Phe Val Arg Phe Asp
355 360 365
Ser Ser Ala Ala Pro Gly Lys Lys Tyr Asn Pro Gly Ser Gln Glu Phe
370 375 380
Ala Thr Leu Thr Gln Ser Ile Ala Ala Ala Ala Asp Arg Phe Met Ser
385 390 395 400
Thr Val Gln Tyr His Gln Asn Pro Asn Gly Ser Leu Ser Glu Glu Phe
405 410 415
Asp Arg Ser Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu Thr Trp Ser
420 425 430
His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly Ser Pro Ser
435 440 445
Phe
<210> 142
<211> 468
<212> PRT
<213> 瓶霉（Saksenaea vasiformis）
<400> 142
Met Pro Ser Trp Lys Thr Leu Phe Leu Leu Leu Gly Pro Ile Ala Thr
1 5 10 15
Ala Ala Ala Ala Pro Val Asp Lys Gln Ser Leu Pro Thr Gly Asn Ser
20 25 30
Thr Ile Ser Ser Trp Val Ser Lys Gln Glu Asp Ile Ser Phe Ser Glu
35 40 45
Met Leu Arg Asn Val Asn Pro Glu Gly Thr Ala Lys Gly Phe Val Ala
50 55 60
Ala Ser Leu Ser Thr Ala Gly Pro Asp Tyr Phe Tyr Thr Trp Thr Arg
65 70 75 80
Asp Ala Ala Leu Val Ser Arg Val Ile Ala Tyr Lys Tyr Asn Thr Thr
85 90 95
Asn Ala Gly Asp Ser Lys Ile His Gly Val Leu Asp Asp Tyr Val Asn
100 105 110
Phe Gln Ile Asn Thr Gln Ser Glu Ser Thr Pro Cys Asn Cys Leu Gly
115 120 125
Glu Pro Lys Phe Asn Pro Asp Gly Ser Ser Phe Thr Gly Pro Trp Gly
130 135 140
Arg Pro Gln Asn Asp Gly Pro Ala Glu Arg Ala Ser Ser Phe Met Leu
145 150 155 160
Ile Ala Asp Ser Phe Leu Ser Gln Thr Lys Asn Ala Ser Tyr Phe Thr
165 170 175
Asn Thr Leu Lys Pro Ala Ile Tyr Lys Asp Leu Asp Tyr Val Val Asp
180 185 190
Thr Trp Ser Asn Pro Cys Phe Asp Leu Trp Glu Glu Val Asn Gly Ile
195 200 205
His Phe Tyr Thr Leu Met Val Met Arg Arg Ser Leu Leu Asp Gly Ala
210 215 220
Asn Phe Ala Thr Arg Asn Gly Asp Asn Ser Lys Ala Ser Thr Tyr Ser
225 230 235 240
Gly Val Ala Ala Lys Ile Gln Ala Arg Leu Asn Ser Phe Trp Asp Ala
245 250 255
Gly Lys Asn Tyr Ile Thr Val Thr Gln Asp Tyr Lys Asn Gly Val Glu
260 265 270
Lys Pro Ser Gly Leu Asp Val Ser Thr Leu Ile Ala Ala Asn Val Ala
275 280 285
Gly Met Gly Asp Gly Phe Tyr Thr Pro Gly Ser Glu Arg Ile Leu Ala
290 295 300
Thr Ala Leu Ala Phe Glu Lys Ser Met Ala Ser Leu Tyr Pro Leu Asn
305 310 315 320
Asn Asn Leu Pro Ser His Leu Gly Asn Ala Ile Gly Arg Tyr Pro Glu
325 330 335
Asp Thr Tyr Asn Gly Asn Gly Asn Ser Gln Gly Asn Pro Trp Phe Leu
340 345 350
Ala Thr Thr Ala Phe Thr Glu Leu Tyr Tyr Arg Ala Ile Leu Glu Trp
355 360 365
Lys Asn Thr Gly Val Thr Val Thr Pro Ile Ser Lys Asp Phe Phe Val
370 375 380
Arg Phe Asp Ser Ser Ala Ala Pro Gly Lys Lys Tyr Asn Pro Gly Ser
385 390 395 400
Gln Glu Phe Ala Thr Leu Thr Gln Ser Ile Ala Ala Ala Ala Asp Arg
405 410 415
Phe Met Ser Thr Val Gln Tyr His Gln Asn Pro Asn Gly Ser Leu Ser
420 425 430
Glu Glu Phe Asp Arg Ser Ser Gly Tyr Met Thr Gly Ala Arg Asp Leu
435 440 445
Thr Trp Ser His Ala Ala Phe Ile Thr Ala Ala Gln Ala Arg Ala Gly
450 455 460
Ser Pro Ser Phe
465

Claims (53)

1.一种用于糖化淀粉底物的方法，所述方法包括将所述淀粉底物与葡糖淀粉酶接触，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种序列基序的多肽，所述序列基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
2.如权利要求1所述的方法，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
3.如权利要求2所述的方法，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
4.如权利要求1-3中任一项所述的方法，其中所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
5.如权利要求4所述的方法，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、V S66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
6.如权利要求1-5中任一项所述的方法，其中所述多肽包含SEQ IDNO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ IDNO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQ IDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ IDNO:79、SEQ IDNO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ ID NO:85、SEQ IDNO:86、SEQ ID NO:87、SEQ IDNO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQ IDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ IDNO:97、SEQ IDNO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQ IDNO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
7.如权利要求1-6中任一项所述的方法，其中所述淀粉底物是约15％至65％、15％至60％或15％至35％的干固体(DS)。
8.如权利要求1-7中任一项所述的方法，其中所述淀粉底物包含液化淀粉、糊化淀粉或颗粒状淀粉。
9.如权利要求1-8中任一项所述的方法，所述方法进一步包括向所述淀粉底物中添加己糖激酶、木聚糖酶、葡萄糖异构酶、木糖异构酶、磷酸酶、植酸酶、支链淀粉酶、β-淀粉酶、α-淀粉酶、蛋白酶、纤维素酶、半纤维素酶、脂肪酶、角质酶、海藻糖酶、异淀粉酶、氧化还原酶、酯酶、转移酶、果胶酶、水解酶、α-葡糖苷酶、β-葡糖苷酶或其组合。
10.如权利要求1-9中任一项所述的方法，其中糖化所述淀粉底物产生高葡萄糖糖浆，所述高葡萄糖糖浆包含一定量的选自由以下组成的组的葡萄糖：至少95.5％葡萄糖、至少95.6％葡萄糖、至少95.7％葡萄糖、至少95.8％葡萄糖、至少95.9％葡萄糖、至少96％葡萄糖、至少96.1％葡萄糖、至少96.2％葡萄糖、至少96.3％葡萄糖、至少96.4％葡萄糖、至少96.5％葡萄糖和至少97％葡萄糖。
11.如权利要求1-10中任一项所述的方法，所述方法进一步包括将所述高葡萄糖糖浆发酵成终产物。
12.如权利要求11所述的方法，其中糖化和发酵作为同时糖化和发酵(SSF)工艺进行。
13.如权利要求11或12所述的方法，其中所述终产物是醇，任选地乙醇。
14.如权利要求11或12所述的方法，其中所述终产物是选自由以下组成的组的生物化学品：氨基酸、有机酸、柠檬酸、乳酸、琥珀酸、谷氨酸一钠、葡糖酸、葡萄糖酸钠、葡萄糖酸钙、葡萄糖酸钾、葡萄糖酸δ-内酯、异抗坏血酸钠、ω3脂肪酸、丁醇、赖氨酸、衣康酸、1,3-丙二醇、生物柴油和异戊二烯。
15.一种由淀粉底物生产发酵产物的工艺，所述工艺包括以下步骤：
1)液化所述淀粉底物；
2)糖化所述液化的淀粉底物；以及
3)用发酵生物发酵；
其中至少使用选自由以下组成的组的葡糖淀粉酶进行步骤2)：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
16.如权利要求15所述的工艺，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
17.如权利要求16所述的工艺，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
18.如权利要求15-17中任一项所述的工艺，其中所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
19.如权利要求18所述的工艺，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
20.如权利要求15-19中任一项所述的工艺，其中所述多肽包含SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQIDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ IDNO:83、SEQ ID NO:84、SEQ ID NO:85、SEQID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQIDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ IDNO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ IDNO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQIDNO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ IDNO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ IDNO:142。
21.一种由淀粉底物生产发酵产物的工艺，所述工艺包括以下步骤：
1)在低于所述淀粉底物的初始糊化温度的温度糖化所述淀粉底物；以及
2)用发酵生物发酵，
其中至少使用选自由以下组成的组的葡糖淀粉酶进行步骤1)：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
22.如权利要求21所述的工艺，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
23.如权利要求22所述的工艺，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
24.如权利要求21-23中任一项所述的工艺，其中所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
25.如权利要求24所述的工艺，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
26.如权利要求21-25中任一项所述的工艺，其中所述多肽包含SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQIDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ IDNO:83、SEQ ID NO:84、SEQ ID NO:85、SEQID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQIDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ IDNO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ IDNO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQIDNO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ IDNO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ IDNO:142。
27.一种用于提高在动物中的淀粉消化率的方法，所述方法包括添加至少一种葡糖淀粉酶，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
28.如权利要求27所述的方法，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
29.如权利要求28所述的方法，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
30.如权利要求27-29中任一项所述的方法，其中所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
31.如权利要求30所述的方法，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
32.如权利要求27-31中任一项所述的方法，其中所述多肽包含SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQIDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ IDNO:83、SEQ ID NO:84、SEQ ID NO:85、SEQID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQIDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ IDNO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ IDNO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQIDNO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ IDNO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ IDNO:142。
33.一种生产发酵饮料的方法，其中所述方法包括使醪液和/或麦芽汁与葡糖淀粉酶接触的步骤，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
34.如权利要求33所述的方法，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
35.如权利要求34所述的方法，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
36.如权利要求33-35中任一项所述的方法，其中所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
37.如权利要求36所述的方法，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
38.如权利要求33-37中任一项所述的方法，其中所述多肽包含SEQ ID NO:61、SEQ IDNO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQ IDNO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQIDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ IDNO:83、SEQ ID NO:84、SEQ ID NO:85、SEQID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQIDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ IDNO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ IDNO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQIDNO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ IDNO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ IDNO:142。
39.一种包含淀粉底物和葡糖淀粉酶的组合物，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性；其中所述组合物的温度是约4℃-40℃并且pH是约3-7。
40.如权利要求39所述的组合物，其中所述多肽在对应于SEQ IDNO:61的多肽的位置102的位置处包含取代、缺失或添加。
41.如权利要求40所述的组合物，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
42.如权利要求39-41中任一项所述的组合物，其中所述多肽在对应于SEQ ID NO:61的多肽的位置66的位置处包含取代、缺失或添加。
43.如权利要求42所述的组合物，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
44.如权利要求39-43中任一项所述的组合物，其中所述多肽包含SEQ ID NO:61、SEQID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ IDNO:65、SEQ ID NO:66、SEQ ID NO:67、SEQID NO:68、SEQ ID NO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ ID NO:73、SEQIDNO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ ID NO:78、SEQ ID NO:79、SEQID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ IDNO:83、SEQ ID NO:84、SEQ ID NO:85、SEQID NO:86、SEQ ID NO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ ID NO:91、SEQIDNO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ ID NO:96、SEQ ID NO:97、SEQID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ IDNO:101、SEQ ID NO:102、SEQ ID NO:103、SEQ ID NO:104、SEQ IDNO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQIDNO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ IDNO:135、SEQ ID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ IDNO:142。
45.一种包含葡糖淀粉酶的重组宿主细胞，所述葡糖淀粉酶选自由以下组成的组：
a)具有与SEQ ID NO:61或SEQ ID NO:142具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的氨基酸序列的多肽；
b)与SEQ ID NO:81具有至少70％、至少75％、至少80％、至少85％、至少90％、至少91％、至少92％、至少93％、至少94％、至少95％、至少96％、至少97％、至少98％、或至少99％同一性的多肽；以及
c)包含一种或多种签名基序的多肽，所述签名基序选自由以下组成的组：
(i)YXaXbTXXXcXd(SEQ ID NO:113)，其中X是任何氨基酸并且Xa是N或S；Xb是T、S、或R；Xc是G或N；并且Xd是D、N、或S；
(ii)YNTTXAGD(SEQ ID NO:114)，其中X是任何氨基酸；
(iii)XaXbXcXcAANXXd(SEQ ID NO:115)，其中X是任何氨基酸并且Xa是S或A；Xb是T、N、或V；Xc是L或I；并且Xd是A或G；
(iv)STLIAANXA(SEQ ID NO:116)，其中X是任何氨基酸；
(v)XaGXGNXbXc(SEQ ID NO:117)，其中X是任何氨基酸并且Xa是N或D；Xb是S或G；并且Xc是Q、K、或E；以及
(vi)NGNGNSQ(SEQ ID NO:118)；
其中所述多肽与SEQ ID NO:61的催化结构域具有至少70％同一性。
46.如权利要求45所述的重组宿主细胞，其中所述多肽在对应于SEQ ID NO:61的多肽的位置102的位置处包含取代、缺失或添加。
47.如权利要求46所述的重组宿主细胞，其中所述多肽包含取代，所述取代选自由以下组成的组：S102P、S102G、S102A、S102V、S102L、S102I、S102F、S102Y、S102W、S102S、S102T、S102C、S102M、S102N、S102Q、S102D、S102E、S102K、S102R、和S102H。
48.如权利要求45-47中任一项所述的重组宿主细胞，其中所述多肽在对应于SEQ IDNO:61的多肽的位置85的位置处包含取代、缺失或添加。
49.如权利要求48所述的重组宿主细胞，其中所述多肽包含取代，所述取代选自由以下组成的组：V66P、VS66G、V66A、V66L、V66I、V66F、V66Y、V66W、V66S、V66T、V66C、V66M、V66N、V66Q、V66D、V66E、V66K、V66R、和V66H。
50.如权利要求45-49中任一项所述的重组宿主细胞，其中所述多肽包含SEQ ID NO:61、SEQ ID NO:62、SEQ ID NO:63、SEQ ID NO:64、SEQ ID NO:65、SEQ ID NO:66、SEQ IDNO:67、SEQ ID NO:68、SEQ IDNO:69、SEQ ID NO:70、SEQ ID NO:71、SEQ ID NO:72、SEQ IDNO:73、SEQ ID NO:74、SEQ ID NO:75、SEQ ID NO:76、SEQ ID NO:77、SEQ IDNO:78、SEQ IDNO:79、SEQ ID NO:80、SEQ ID NO:81、SEQ ID NO:82、SEQ ID NO:83、SEQ ID NO:84、SEQ IDNO:85、SEQ ID NO:86、SEQ IDNO:87、SEQ ID NO:88、SEQ ID NO:89、SEQ ID NO:90、SEQ IDNO:91、SEQ ID NO:92、SEQ ID NO:93、SEQ ID NO:94、SEQ ID NO:95、SEQ IDNO:96、SEQ IDNO:97、SEQ ID NO:98、SEQ ID NO:99、SEQ ID NO:100、SEQ ID NO:101、SEQ ID NO:102、SEQID NO:103、SEQ ID NO:104、SEQ ID NO:119、SEQ ID NO:120、SEQ ID NO:121、SEQ ID NO:122、SEQ ID NO:123、SEQ ID NO:126、SEQ ID NO:129、SEQ ID NO:132、SEQ ID NO:135、SEQID NO:138、SEQ ID NO:140、SEQ ID NO:141、或SEQ ID NO:142。
51.如权利要求45-50中任一项所述的重组宿主细胞，所述重组宿主细胞是产乙醇微生物。
52.如权利要求51所述的重组宿主细胞，所述重组宿主细胞是酵母细胞。
53.如权利要求45-52中任一项所述的重组宿主细胞，其中所述宿主细胞不是瓶霉(Saksenaea vasiformis)。
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