CN115851556B - Streptococcus agalactiae and application thereof - Google Patents
Streptococcus agalactiae and application thereof Download PDFInfo
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- CN115851556B CN115851556B CN202310133921.4A CN202310133921A CN115851556B CN 115851556 B CN115851556 B CN 115851556B CN 202310133921 A CN202310133921 A CN 202310133921A CN 115851556 B CN115851556 B CN 115851556B
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Abstract
The invention discloses non-lactose-decomposing streptococcus and application thereof, and relates to the technical field of microorganisms. The non-lactose-decomposing streptococcusStreptococcus alactolyticus) The non-lactose-decomposing streptococcus strain PD-O-14 and the protein feed are fed together to a mouse, so that the content of various essential amino acids in jejunal chyme of the mouse can be improved, crude protein is converted into various essential amino acids, the intake of the essential amino acids of the mouse is improved, the addition of the essential amino acids in the protein feed can be reduced, and the feed cost is reduced.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to non-lactose-decomposing streptococcus and application thereof.
Background
The essential amino acid is an amino acid which cannot be synthesized by an animal or has a synthesis rate which is far from suitable for the needs of an organism and must be taken in from the outside.
The species of animals vary, as do the required essential amino acid species. Such as: total 8 essential amino acids for adults; pigs have 10 kinds of essential amino acids in total; chickens have a total of 11 essential amino acids.
In the conventional feed for livestock and poultry, the raw material components such as bean pulp, fish meal and the like often lack enough essential amino acids, and in order to ensure the normal growth of livestock and poultry animals, the breeders need to add artificially synthesized essential amino acids into the feed. The price of the artificially synthesized essential amino acid is high, and the cultivation cost can be greatly increased. It can be seen that the prior art is in need of improvement.
Disclosure of Invention
In view of the above-mentioned shortcomings of the prior art, it is an object of the present invention to provide a streptococcus agalactiae and its use, aimed at increasing the intake of essential amino acids in animals.
In order to achieve the above purpose, the invention adopts the following technical scheme:
a non-lactose-decomposing streptococcus which is [ ] aStreptococcus alactolyticus) The strain is named as PD-O-14, wherein the strain is preserved in the microorganism strain preservation center of Guangdong province at the 10 th month 13 of 2022, and the preservation number is GDMCC NO.62865.
The application of the streptococcus agalactiae in preparing animal protein feed.
The application of the streptococcus agalactiae in preparing pig protein feed.
The application of the streptococcus agalactiae in preparing poultry protein feed.
The application of the non-lactose-degrading streptococcus in preparing intestinal probiotics.
The use of said non-lactose degrading streptococcus for increasing the concentration of essential amino acids in animal intestinal chyme.
The beneficial effects are that: the invention provides a non-lactose-decomposing streptococcus which is named as PD-O-14 and can improve the content of various essential amino acids in jejunal chyme of a mouse fed with protein feed. The non-lactose-decomposing streptococcus strain PD-O-14 can convert crude protein into a plurality of essential amino acids in the intestinal tract of an animal, so that the intake of the essential amino acids of the animal is improved.
The non-lactose-decomposing streptococcus strain PD-O-14 can be used by being compounded with protein feed, so that the intake of essential amino acids of animals after eating the protein feed is improved, and the addition of the essential amino acids in the feed is reduced.
The streptococcus agalactiae can also be applied to preparing intestinal probiotics, and after the animal eats protein feed, the bacteria containing the streptococcus agalactiae strain PD-O-14 can be taken, so that the content of various essential amino acids in the intestinal chyme of the animal can be improved.
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FIG. 1 shows the results of detection of methionine synthesis pathway-associated enzyme gene of strain PD-O-14.
FIG. 2 shows the results of detection of leucine synthesis pathway-related enzyme genes of strain PD-O-14.
FIG. 3 shows the results of detection of tryptophan synthesis pathway related enzyme genes of strain PD-O-14.
FIG. 4 shows the results of detection of threonine-pathway-associated enzyme genes of strain PD-O-14.
FIG. 5 shows the results of detection of valine synthesis pathway-associated enzyme gene of strain PD-O-14.
FIG. 6 shows the results of detection of isoleucine-synthesis pathway-related enzyme genes of strain PD-O-14.
Fig. 7 is a comparison of valine abundance in mouse jejunal chyme of LC and LS groups.
Fig. 8 is a comparison of the abundance of tyrosine in mouse jejunal chyme in LC and LS groups.
Fig. 9 is a comparison of the abundance of isoleucine in mouse jejunal chyme in LC and LS groups.
Fig. 10 is a comparison of methionine abundance in jejunal chyme in mice of LC and LS groups.
Fig. 11 is a comparison of the abundance of phenylalanine in the mouse jejunal chyme of LC and LS groups.
Fig. 12 is a comparison of tryptophan abundance in jejunal chyme in mice of LC and LS groups.
Detailed Description
The invention provides a streptococcus agalactiae and application thereof, and further details of the invention are described below with reference to the accompanying drawings and examples in order to make the purpose, technical scheme and effect of the invention clearer and more definite. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Example 1 screening and identification of strains
1. 7 parts of panda feces are mixed, sterile PBS is added to 45ml, and then the mixture is fully shaken, and then the bacterial suspension is diluted to 100 times and plated. The culture was carried out at 37℃for 24 hours in each of aerobic and anaerobic conditions.
The culture medium adopts brain heart extract agar (BHIA).
The formula of brain-heart extract agar (BHIA) is as follows: animal tissue gastric enzyme digest 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, calf brain extract 12.5g, calf heart extract 5.0g, agar 15.0g, pH 7.4+ -0.2 (25 ℃).
2. And (3) selecting a single colony on a flat plate for identification, extracting DNA of the separated strain, carrying out 16S rDNA sequencing by adopting a sanger sequencing method, and carrying out colony classification identification on a sequencing result by on-line blast comparison (https:// blast. Ncbi.lm. Nih. Gov). The non-lactose-decomposing streptococcus strains are selected for preservation, 12 non-lactose-decomposing streptococcus strains are obtained in the screening and separation, and the same strains are found through multiple comparison (forward sequence). The non-lactose-decomposing streptococcus strain with the strain number of PD-O-14 is taken and sent to the Guangdong province microorganism strain collection center for preservation.
EXAMPLE 2 Whole genome assay
The whole genome sequence of the strain PD-O-14 is determined (sequencing company is Beijing qing department biotechnology Co., ltd.), and the determination result shows that the strain PD-O-14 comprises an integral anabolic pathway of eight human essential amino acids and also comprises a KEGG metabolic pathway of three synthetic essential amino acids such as map00290, map00300 and map00400, which shows that the strain PD-O-14 has the capability of synthesizing eight human essential amino acids. FIGS. 1 to 6 show the results of detection of a pathway-related enzyme gene for synthesis of a part of essential amino acids in human body by strain PD-O-14. Taking fig. 1 as an example, the numbers inside the boxes in the figure represent the EC numbers of the enzymes, wherein the underlined boxes represent the genes encoding the enzymes contained in the PD-O-14 genome, and the blank boxes without the ground color represent the genes encoding the enzymes were not detected.
FIG. 1 shows the results of detection of methionine synthesis pathway-associated enzyme genes of strain PD-O-14, in which only the gene encoding the enzyme having EC number 4.4.1.13 was not detected in strain PD-O-14, and the remaining genes encoding enzymes involved in methionine synthesis were detected, indicating that strain PD-O-14 has the possibility of synthesizing methionine.
FIGS. 2 to 5 show the results of detection of leucine, tryptophan, threonine and valine synthesis pathway-related enzyme genes of the strain PD-O-14, respectively, and show that the genes encoding the enzymes involved in leucine, tryptophan, threonine and valine synthesis can be detected, indicating that the strain PD-O-14 has the ability to synthesize leucine, tryptophan, threonine and valine.
FIG. 6 shows the results of detection of the enzyme gene associated with the isoleucine synthesis pathway of strain PD-O-14, which shows that the gene encoding the enzyme with EC number 2.3.3.21 is not detected in strain PD-O-14, indicating that the intermediate metabolite 2-oxobutyrate may not be synthesized by the pyruvate pathway, but it may be obtained by threonine synthesis, indicating that strain PD-O-14 also has at least one complete isoleucine synthesis pathway.
In addition, all genes encoding enzymes involved in lysine, phenylalanine and tyrosine synthesis were detected in the strain PD-O-14.
Example 3 animal experiments
24 female BALB/c mice were purchased from Guangzhou Qing le life sciences Co., ltd. Mice were treated withPlacing in standard cages (1 per cage), and pre-feeding with corncob and wood shavings as padding at 25deg.C. After 7 days of pre-feeding, the mice were randomly divided into LC and LS groups of 12 mice each. LC and LS mice were fed low protein feed with a crude protein content of 10% and were continuously fed with stomach (1 time daily) for seven days, wherein LS mice were fed with 0.5mL of a culture broth of Streptococcus agalactiae strain PD-O-14 having a cell concentration of 1X 10 8 CFU/mL; LC group mice served as negative controls and were perfused with 0.5mL of sterile PBS (phosphate buffer).
The mice were given protein feed (2.6 g) in an amount of about 70% of the required during the trial period, based on the daily feed requirements of the mice under free feeding conditions during the pre-feed period. After the 21d collection of the mouse faeces after the stomach filling, the mice are dislocation killed, then intestinal chyme in the jejunum of the mice is collected and stored in a 2ml cryopreservation tube, the cryopreservation tube is placed in liquid ammonia for quick freezing, and then the mice are transferred into a refrigerator at the temperature of minus 80 ℃ for preservation, and the mice are used for sequencing of metabonomics.
EXAMPLE 4 metabolite profiling
The detection of non-targeted metabonomics of jejunal chyme was performed by the Beijing norelvan source technologies Co.
Sample preparation: jejunal chyme samples (100 mg) were first separately ground with liquid nitrogen and then resuspended in pre-frozen 80% methanol and 0.1% formic acid for homogenization. The treated sample was incubated at 4℃for 5 minutes, centrifuged at 4℃for 5 minutes at a high speed (15,000 r/min), the supernatant was collected, and the supernatant was diluted with liquid chromatography mass spectrometry (LC-MS) grade water to a final concentration of 53% methanol in the supernatant, thereby completing the preparation of the sample.
The prepared samples were subjected to non-targeted metabonomic analysis using an LC-MS system and data were determined using a UHPLC system (Thermo Fisher Scientific, germany) and an Orbitrap Q ACTIVETM HF mass spectrometer (Thermo Fisher Scientific). The raw data were further processed using compound finder 3.1 (CD 3.1, thermo Fisher Scientific) to obtain a quantification of the metabolites.
The corresponding measurement results are as follows:
in the metabonomics of mouse jejunum chyme, 5 of 8 essential amino acids required by the human body were detected, valine, isoleucine, methionine, phenylalanine and tryptophan, respectively, and 1 of the conditionally essential amino acids, tyrosine, were also detected.
Fig. 7 shows comparison of the abundance of valine in the jejunal chyme of mice in LC and LS groups, and from the results in the figures, the abundance of valine in the jejunal chyme of mice in LS group is significantly higher than that in LC group (p=0.039), which indicates that the valine content in jejunal chyme can be significantly increased and the valine absorption capacity of mice can be increased by feeding the mice with the bacterial liquid of the non-lactose degrading streptococcus strain PD-O-14 while feeding the low protein feed. Valine is a common essential amino acid for human, chicken and pigs, is the 4 th restriction amino acid of broiler chickens, and is the 5 th restriction amino acid of pigs. Valine also significantly increases milk production for lactating sows, and is a major limiting amino acid in lactating sow feed.
Fig. 8 is a comparison of the abundance of tyrosine in the jejunal chyme of mice in LC and LS groups, and from the results in the figures, it can be seen that the abundance of tyrosine in the jejunal chyme of mice in LS group is significantly higher than that in LC group (p=0.027), which indicates that feeding the mice with the bacterial liquid of the streptococcus non-lactose-dissolving strain PD-O-14 while feeding the low protein feed can significantly increase the content of tyrosine in jejunal chyme, thereby increasing the tyrosine absorption of mice. Tyrosine is a necessary amino acid, and tyrosine is converted from phenylalanine, which is an essential amino acid, so that the requirement of animals for phenylalanine intake can be reduced by increasing the intake of tyrosine. Tyrosine is an essential amino acid for chicks.
Fig. 9 is a comparison of the abundance of isoleucine in the mice jejunum of LC and LS groups, and from the results in the figure, it can be seen that the abundance of isoleucine in the mice jejunum of LS group is higher than that of LC group (p=0.059), indicating that the content of isoleucine in the mice jejunum can be increased by feeding the mice with the bacterial liquid of the non-lactose degrading streptococcus strain PD-O-14 while feeding the mice with the low protein feed. Isoleucine is a limiting amino acid of low-protein feeds, and the deletion of isoleucine can lead to the reduction of feed intake of nursery pigs, and can also have significant influence on feed intake and weight gain of piglets.
Fig. 10 is a comparison of the abundance of methionine in the mice jejunum of LC and LS groups, and from the results in the figures, it can be seen that the abundance of methionine in the mice jejunum of LS group is higher than that in LC group (p=0.068), indicating that the methionine content in the mice jejunum can be increased by feeding the mice with the bacterial liquid of the non-lactose degrading streptococcus strain PD-O-14 while feeding the mice with the low protein feed. Methionine is the second limiting amino acid of pigs and is also the first limiting amino acid of poultry ration, and is commonly absent in vegetable protein feeds, so that additional methionine is commonly required in ration. The non-lactose-dissolving streptococcus strain PD-O-14 can improve the methionine content in the jejunal chyme of animals, reduce the methionine addition amount in protein feed and reduce the feed cost.
Fig. 11 is a comparison of the abundance of phenylalanine in the mice jejunal chyme of LC and LS groups, and from the results in the figures, it can be seen that the abundance of phenylalanine in the mice jejunal chyme of LS group is higher than that in LC group (p=0.37), indicating that the content of phenylalanine in the mice jejunal chyme can be increased by feeding the mice with the bacterial liquid of the non-lactose degrading streptococcus strain PD-O-14 while feeding the mice with the low protein feed.
Fig. 12 is a comparison of the tryptophan abundance in the mice jejunum of LC and LS groups, and from the results in the graph, it can be seen that the tryptophan abundance in the mice jejunum of LS group is higher than that in LC group (p=0.28), indicating that the tryptophan content in the mice jejunum can be increased by feeding the mice with the bacterial liquid of the non-lactose degrading streptococcus strain PD-O-14 while feeding the mice with the low protein feed.
Tryptophan and phenylalanine are essential amino acids in the growth process of chickens and pigs, so that proper amounts of tryptophan and phenylalanine are added into daily ration of chickens and pigs, and the measurement results show that the content of various essential amino acids in jejunum chyme of mice fed with the non-lactose degrading streptococcus strain PD-O-14 bacterial liquid is increased, wherein the abundance of valine and tyrosine is obviously increased relative to that of a negative control LC group, and the abundance of isoleucine and methionine is also obviously increased. While the abundance of phenylalanine and tryptophan in the LS group was not significantly increased compared to the LC group, the tendency to enrichment also occurred, with higher abundance. The non-lactose-decomposing streptococcus strain PD-O-14 can convert and utilize crude protein in the intestinal tract environment of mice to generate various essential amino acids, so that the intake of the essential amino acids of animals is improved.
The non-lactose-degrading streptococcus strain PD-O-14 acts after entering the intestinal tract of a mouse, and in practical application, the non-lactose-degrading streptococcus strain PD-O-14 can be compounded with protein feed to form protein feed containing the non-lactose-degrading streptococcus strain PD-O-14.
The non-lactose-decomposing streptococcus strain PD-O-14 can be independently used as a probiotic bacterial agent for animals to eat, so that the addition of various essential amino acids in protein feed can be reduced, the cost of the protein feed is reduced, and the low-protein feed is facilitated. The low-protein feed can reduce nitrogen emission in the cultivation process, reduce sewage and waste gas generation, and improve the utilization rate of protein in the feed. The non-lactose-decomposing streptococcus strain PD-O-14 is matched with low-protein feed for animals to eat, so that the deficiency of essential amino acids in the low-protein feed can be effectively overcome.
It will be understood that equivalents and modifications will occur to those skilled in the art in light of the present invention and their spirit, and all such modifications and substitutions are intended to be included within the scope of the present invention as defined in the following claims.
Claims (5)
1. A non-lactose-decomposing streptococcus is characterized in thatStreptococcus alactolyticus) The strain is named as PD-O-14 and is deposited with the microorganism strain collection of Guangdong province on the 10 th month 13 of 2022 under the deposit number GDMCC No.62865.
2. Use of the non-lactose degrading streptococcus according to claim 1 for preparing animal protein feed.
3. Use of the non-lactose degrading streptococcus according to claim 1 for preparing pig protein feed.
4. Use of the non-lactose degrading streptococcus according to claim 1 for preparing a poultry protein feed.
5. Use of the non-lactose degrading streptococcus according to claim 1 for preparing a bacterial agent.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805041A (en) * | 2015-04-03 | 2015-07-29 | 中国农业科学院兰州畜牧与兽药研究所 | Streptococcus alactolyticus strain and application thereof |
| JP2020191793A (en) * | 2019-05-24 | 2020-12-03 | 日環科学株式会社 | Experiment non-human animal and use thereof |
| CN112273558A (en) * | 2019-07-24 | 2021-01-29 | 武汉天龙饲料有限公司 | Low-protein and amino acid-balanced biological feed and preparation method thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104805041A (en) * | 2015-04-03 | 2015-07-29 | 中国农业科学院兰州畜牧与兽药研究所 | Streptococcus alactolyticus strain and application thereof |
| JP2020191793A (en) * | 2019-05-24 | 2020-12-03 | 日環科学株式会社 | Experiment non-human animal and use thereof |
| CN112273558A (en) * | 2019-07-24 | 2021-01-29 | 武汉天龙饲料有限公司 | Low-protein and amino acid-balanced biological feed and preparation method thereof |
Non-Patent Citations (5)
| Title |
|---|
| Jingyan Zhang et al..The safety and potential probiotic properties analysis of Streptococcus alactolyticus strain FGM isolated from the chicken cecum.《Annals of Microbiology 》.2021,第71卷(第1期),第1-14页. * |
| Kelang Kang et al..Multi-Omics Analysis of the Microbiome and Metabolome Reveals the Relationship Between the Gut Microbiota and Wooden Breast Myopathy in Broilers.《The veterinarian friendly holds the cat's paw in the river outdoors. The concept of a veterinary clinic. Doctor services for animals, treatment of pets. Frontiers in Veterinary Science》.2022,第9卷第1-13页. * |
| 刘璐 等.饲粮蛋白质水平降低对成年大鼠生长性能、氮平衡和骨骼肌蛋白质周转的影响.动物营养学报.2019,第31卷(第5期),第2222-2231页. * |
| 吴开开 等.非解乳糖链球菌FGM对大肠杆菌感染肉鸡肠道健康的影响.中国兽医科学.2020,第50卷(第5期),第631-637页. * |
| 韩佳临.种猪肠道微生物分析、益生菌筛选及发酵豆粕的初步研究.《中国优秀硕士学位论文全文数据库 基础科学辑》.2021,(第7期),A006-225. * |
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