CN115845011B - Traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver and preparation method thereof - Google Patents
Traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which comprises 3-6 parts of kudzuvine root, 3-8 parts of hovenia dulcis thunb and 1-5 parts of Tibetan tea; 1 to 3 parts of cogongrass rhizome; 2-5 parts of mulberry and 1-3 parts of liquorice; 0.5 to 2 parts of dried orange peel and 1 to 3 parts of tuckahoe; 1 to 3 parts of medlar; 2 to 5 parts of cardamon, 1 to 3 parts of alisma orientale and 1 to 3 parts of coix seed; 1 to 3 parts of eupatorium; 1 to 3 parts of ginseng; 1 to 3 parts of platycodon grandiflorum; 0.5 to 1 part of madder root and 1 to 5 parts of perilla; 1 to 3 parts of pipewort; 1 to 3 parts of lycopodium clavatum. The invention also discloses a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition disclosed by the invention is prepared according to the characteristics among medicines, and the traditional Chinese medicine composition is synergistic mutually, so that the anti-alcohol effect can be achieved, the activity of acetaldehyde dehydrogenase can be improved, the accumulation effect of acetaldehyde in the liver can be reduced, and the liver can be protected while the anti-alcohol effect can be improved.
Description
Technical Field
The invention relates to the technical field of anti-alcoholic traditional Chinese medicines, in particular to a traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver.
Background
Alcohol is a common living habit of people, ethanol in alcoholic beverages is mainly absorbed in the stomach of people, and because ethanol is a fat-soluble small molecule, the ethanol rapidly enters the blood circulation system of the human body in an active diffusion mode, so that the reaction after drinking is faster.
Alcohol is absorbed into blood by gastrointestinal tract after entering human body, most of the alcohol is metabolized in liver, and only 2% -10% of alcohol is excreted by kidney and lung. In the liver, alcohol first turns into acetaldehyde and then into acetic acid. Among them, acetaldehyde is the main cause of liver damage, and its toxicity is 15 times that of ethanol. Therefore, the liver must immediately convert acetaldehyde into acetic acid which is nontoxic to the human body, and finally oxidize the acetaldehyde into carbon dioxide and water to be discharged outside the body.
Human cells secrete two enzymes during alcohol metabolism, one of which is called alcohol dehydrogenase ADH, which converts ethanol to acetaldehyde using Zn as an active center. Another is the aldehyde dehydrogenase ALDH, which converts acetaldehyde to acetic acid using cysteine (Cys) residues. The alcohol dehydrogenase and the acetaldehyde dehydrogenase can catalyze reversible reactions with Nicotinamide Adenine Dinucleotide (NAD) as a coenzyme.
The alcohol dehydrogenase in the liver is responsible for oxidizing alcohol to acetaldehyde, and the generated acetaldehyde is further used as a substrate to be converted into harmless acetic acid under the catalysis of the acetaldehyde dehydrogenase. Acetaldehyde toxicity is higher than ethanol, and is one of the main causes of hangover. The acetaldehyde conversion in human body is mainly acetaldehyde dehydrogenase (ALDH) in liver, the ALDH1 and the ALDH2 have obvious difference in catalysis rate, the KM of the ALDH2 to acetaldehyde is lower than that of the ALDH1, about 1/10 of the latter is the isozyme mainly responsible for acetaldehyde conversion.
In the conversion of ethanol, acetaldehyde and acetic acid, the biochemical efficiency of the conversion of acetaldehyde to acetic acid is actually lower than the efficiency of the conversion of ethanol to acetaldehyde; after alcohol dehydrogenase ADH rapidly converts ethanol into acetaldehyde, acetaldehyde cannot be rapidly converted into acetic acid, so that acetaldehyde accumulates in the liver, and finally, the liver is damaged by acetaldehyde.
Acetaldehyde has high toxicity and can react with protein, and the nearby liver cells are firstly attacked, so that people drinking wine to hurt the liver and people drinking wine to flush are more likely to hurt the liver. Not only can the gene inhibit the activity of acetaldehyde dehydrogenase, but also some medicines such as cefuroxime can inhibit the activity of acetaldehyde dehydrogenase, so that the acetaldehyde content in the human body is excessive and toxic.
For biochemical reactions of the human body, the reaction efficiency of the enzymatic reaction is affected by the enzyme concentration, the enzyme activity and the reaction environment. Both the enzyme concentration and the enzyme activity can be influenced by gene expression, and the reaction environment can also have a great influence on the enzyme activity. The prior art discloses that the effects of alleviating hangover and protecting liver are achieved by regulating the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase.
Document 1: a medicinal preparation containing herba Cichorii for relieving alcoholic intoxication and protecting liver, and its preparation method
The following technical matters are disclosed in document 1: the invention utilizes the enzymolysis method to prepare the micromolecular oligopeptide with high efficiency and simple process, fully utilizes the abandoned soluble chicory protein during the conventional extraction of chicory polysaccharide, has small molecular weight and easy absorption by human body, is rich in amino acids such as alanine, leucine, glutamic acid and the like, and can obviously improve the content of alanine and leucine in serum by orally taking the anti-alcoholic liver-protecting agent, thereby generating stable alcohol-decomposing auxiliary dehydrogenase, enhancing the activities of alcohol dehydrogenase and aldehyde dehydrogenase of the liver and promoting the decomposition and metabolism of alcohol in vivo.
The anti-alcoholic liver protection agent containing chicory oligopeptide in the document 1 can promote the activity of alcohol dehydrogenase by increasing the content and activity of coenzyme NAD+ and NAD+ of alcohol dehydrogenase by increasing the content of alanine and leucine in serum, thereby facilitating the catabolism of alcohol in vivo, inhibiting the rise of alcohol in blood and achieving the aim of anti-alcoholic. The drug without addition of chicory oligopeptide or only addition of chicory extract does not have the efficacy of efficiently decomposing ethanol, further illustrating the remarkable anti-alcoholic effect of chicory oligopeptide.
It is seen from document 1 that, in document 1, the activity of ADH and ALDH can be improved by adding other small-molecule amino acids such as chicory polysaccharide, and by increasing the content of NAD, which is a co-dehydrogenase in human body, after oral administration, since ADH and ALDH require the participation of NAD during the enzymatic reaction.
However, the coenzyme NAD acts only as a coenzyme in the enzymatic reaction of ethanol and acetaldehyde, and the content of the coenzyme itself is considered, so actually increasing the content of the coenzyme NAD may be able to improve the activity of the enzyme, but not significantly improve the enzymatic reaction.
As is well known, enzyme activity or enzyme activity refers to the amount of enzyme required to convert 1. Mu. Mole of substrate to one unit of enzyme activity in 1 minute under optimal conditions. I.e., the amount of enzyme required to convert a unit substrate in a unit time. The enzyme activity affects the overall rate of the enzymatic reaction, but the rate of the enzymatic reaction is affected by a variety of factors, such as enzyme concentration, substrate concentration, pH, temperature, inhibitors and activators, etc. For example, when the substrate concentration is low, the enzymatic reaction rate increases in a linear proportion as the substrate concentration increases, whereas when the substrate concentration continues to increase, the tendency of the reaction rate to increase gradually becomes gentle, and once the substrate concentration reaches a relatively high level, the reaction rate no longer increases to reach a limit maximum, called the maximum reaction rate. Substances that change from inactive to active or increase the activity of enzymes are called activators (activators are mostly metal ions). Substances that decrease the activity of an enzyme but do not cause denaturation of the enzyme protein are known as inhibitors. Inhibitors can be further classified into reversible inhibitors and irreversible inhibitors. For example, cephalosporin is an enzyme inhibitor, which reacts with alcohol in a disulfiram-like manner, inhibits acetaldehyde dehydrogenase, and inhibits the normal metabolic processes of alcohol, so that even drinking small amounts of alcohol can cause acetaldehyde poisoning.
Document 2: anti-alcohol liver-protecting composition, anti-alcohol liver-protecting beverage and preparation method and application thereof
Disclosed in document 2 are: the high, medium and low dose groups of the anti-alcoholic liver protection composition were administered with different concentrations of the anti-alcoholic liver protection composition solutions (the anti-alcoholic liver protection composition prepared in example 3 was mixed with water to obtain the anti-alcoholic liver protection composition solutions of 1.572g/ml,0.786g/ml and 0.393g/ml, respectively) by the stomach irrigation of 0.1ml/10g 1 hour after the administration, and the administration was continued for 14 d.
The results showed that the mice with different concentrations of the anti-hangover and hepatoprotective composition had elevated ADH activity in the liver, a very significant difference (P < 0.01) with prolonged latency to loss of regular reflection and a shortened recovery time compared to the model group, wherein the recovery time was significantly shortened in the high dose group compared to the model group and a significant difference (P < 0.05) (table 3).
It can be seen from document 2 that the activity of alcohol dehydrogenase in mouse liver is improved by optimizing the formulation in document 2; the activity of alcohol dehydrogenase is improved relative to normal mice; further, alcohol metabolism was improved, but the specific effect on acetaldehyde dehydrogenase was not exhibited in document 2, and it can be seen from table 3 in document 2 that the dose group had a smaller increase in the activity of alcohol dehydrogenase than that of the model group, so that the actual anti-alcoholic effect was general.
Document 3: study of anti-hangover effect of Mel on acute alcoholism rats, author Liang Lulu, etc.
Disclosed in document 3 is: a rat model with alcoholism is constructed by using 75% edible ethanol, 3 concentrations of honey are adopted for gastric lavage intervention, and the serum ethanol concentration, liver ethanol dehydrogenase, acetaldehyde dehydrogenase and the like of the rat are used as investigation indexes. The results prove that the honey can have obvious anti-alcohol effect by improving the ADH and ALDH activities in the liver and the stomach. However, from the experimental data in document 3, the ADH and ALDH activities in the liver were 16-30 times higher than those in the stomach, indicating that the liver is the main site for alleviating hangover.
Document 4: influence of mung bean, honey and yogurt on alcohol dehydrogenase and acetaldehyde dehydrogenase Activity
Disclosed in document 4 is: mice were divided into 4 groups, and mung bean juice, honey water and yogurt were infused into the stomach of the mice, and the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase was measured in each group. As a result, mung beans, honey and yoghurt have certain promotion effects on the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase, the effects are ordered as mung beans, honey and yoghurt respectively, and the mung beans can improve the activities of the alcohol dehydrogenase and the acetaldehyde dehydrogenase by 22.98U/ml and 6.02U/ml respectively.
It is clear from documents 3 and 4 that there are some substances in the prior art that can promote the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase.
Document 5: effect of alcohol consumption on the expression of rat liver alcohol dehydrogenase 1 and acetaldehyde dehydrogenase 2
Document 5 discloses: the wine has different flavor types, different production process, different quality and different content components, and the influence of the drinking white wine on alcohol dehydrogenase ADH and acetaldehyde dehydrogenase ALDH, which are two key enzymes of alcohol metabolism, is discussed by observing whether the metabolic process of the drinking white wine in the body is different from that of pure alcohol.
The experimental results disclosed in document 5 show that ADH and ALDH are expressed with gene polymorphism, 8 isozymes are present in human ADH, and 4 isozymes are present in human ALDH. In this experiment, the alcohol group for medium and high dose experiments and the alcohol group rats showed significantly lower expression of ADH1 compared to the blank control group, whereas the alcohol group for medium and high dose experiments showed no significant difference in ADH1 expression from the alcohol group.
Compared with a blank control group, the activity of ALDH of a middle-high dose experimental wine group and the activity of ALDH of an ethanol group rat are obviously lower, the influence of the experimental wine group on the ALDH and the ethanol group are obviously different, and the ALDH expression of 3 experimental wine groups is higher than that of the ethanol group; the experimental alcoholic group showed lower consumption of ALDH than the alcoholic group during metabolism.
The experiment shows that: ALT, AST, ethanol and acetaldehyde in serum; also, there was a difference between the 3 dose groups of experimental wine with increasing amounts of alcohol consumption, with increasing ALT and AST, and decreasing ADH1 and ALDH2 expression levels, as well as the effect on ADH and ALDH mRNA and protein expression in the liver.
Document 6: the principle of protecting liver and dispelling effects of alcohol of acetaldehyde dehydrogenase with high temperature resistance and acid and alkali resistance is written in the author Ge Xin.
Literature 6 discloses that: the human ADH is mainly beta-chain dimer, comes from ADH2 gene, most white people are ADH2-1, yellow people are ADH2-2, and the enzyme activity generated by ADH2-2 is about 100 times higher than that generated by ADH2-1, so that most white people produce acetaldehyde slowly after drinking, and yellow people produce acetaldehyde faster.
There are more than ten aldehyde dehydrogenases in humans, and acetaldehyde oxidation in humans is mainly accomplished by ALDH1 and ALDH2, whereas ALDH2 is about 10-50 times more active than ALDH 1.
The ALDH2 gene of most eastern species is mutant, i.e., amino acid 487 of ALDH is changed from glutamic acid to lysine, which results in loss of enzymatic activity of ALDH2, which also results in lower ALDH activity in yellow species than white species and more likely to produce symptoms of acetaldehyde poisoning.
From documents 1 to 6, it can be seen that:
(1) Alcohol dehydrogenase ADH and acetaldehyde dehydrogenase ALDH are key enzymes for alcohol metabolism, and in the metabolic process, the enzyme activity, the enzyme concentration and coenzyme NAD can have influence on the metabolic efficiency of the enzymatic reaction; and because the eastern population has the defect of acetaldehyde dehydrogenase gene, the expression of acetaldehyde dehydrogenase is limited, the efficiency of converting acetaldehyde into acetic acid is lower than the efficiency of converting ethanol into acetaldehyde, and the accumulation of acetaldehyde is caused. Because acetaldehyde is far more toxic than ethanol, the activity of acetaldehyde dehydrogenase is a key to protecting the liver.
(2) The anti-alcoholic substances such as mung bean, honey and anti-alcoholic drugs in the prior art can improve the activities of ADH and ALDH in human bodies, and can obviously improve the activities of ADH and ALDH in human bodies after taking the anti-alcoholic substances, for example, in document 4, the liver ADH activity of a normal group is 5.31U/mg, and the liver ADH activity of a honey group is the lowest 12.58U/mg. ADH activity and ALDH activity of the high, medium and low groups taking honey are higher than those of the normal group; however, honey belongs to fructose substances, and after taking the honey, the honey can influence blood sugar, so that diabetics cannot take the honey. Mung beans and yogurt, although not affecting blood sugar, are often used as a food; in the document 4, mung beans are boiled for 10min to obtain mung bean juice, and the mung bean juice has better effect than honey; through experience, the mung beans should be fresh mung beans, and if the mung beans are dried, the mung beans cannot be obtained with corresponding mung bean juice after being boiled for 10 minutes. However, fresh mung beans are not suitable for industrial production because of short storage time, and require additional refrigeration or preservation, and even so, the preservation time is limited, so that the fresh mung beans are inconvenient to produce in pharmaceutical factories.
(3) It can be seen from document 5 that different types of alcohol sources or ethanol can have an effect on the expression of alcohol dehydrogenase 1 and acetaldehyde dehydrogenase 2.
In patent document 1, chicory polysaccharide and chicory coumarin need to be prepared, and the preparation method of the chicory extract is different from the extraction method of other traditional Chinese medicines in the formula, and cannot be prepared by adopting a uniform method, so that the preparation difficulty is greatly increased. For example, in the preparation process, the enzymolysis protein obtained in the enzymolysis liquid is classified by utilizing SephadexG-25, chicory oligopeptide with the molecular weight of 500-1000 Da is collected, and chicory oligopeptide powder is prepared by adopting a vacuum drying method; such process requirements also result in reduced efficiency of the preparation.
In addition, patent document 1 has a problem that the content of alanine and leucine in serum can be significantly increased to produce stable alcohol-decomposing co-dehydrogenase, and the activity of alcohol dehydrogenase is increased by increasing the amount of coenzyme, however, the effect of the amount of coenzyme on the efficiency of alcohol conversion is limited and other interference factors are liable to be received. Patent document 2 can increase the activity of alcohol dehydrogenase in the liver of a mouse, but the effect of the increase is general.
The inventor applies for a medicinal composition for dispelling the effects of alcohol and protecting liver in 2020, and a preparation method and an application of the medicinal composition for dispelling the effects of alcohol and protecting liver are disclosed in the invention patent with the patent number of 2020102771112. The formula of the traditional Chinese medicine composition is further optimized through the adjustment of the inventor, the problems are solved, and the traditional Chinese medicine composition can show better effects of dispelling the effects of alcohol and protecting the liver.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver, which can solve the following technical problems:
(1) The expression of human liver to the acetaldehyde dehydrogenase is improved, the enzymatic reaction efficiency of the acetaldehyde dehydrogenase is improved, and the decomposition efficiency of acetaldehyde in the liver is reduced so as to achieve the effect of protecting the liver.
(2) The production process is simplified, and the industrial production is facilitated; is suitable for more extensive people.
(3) The stimulation degree of alcohol to intestines and stomach is reduced.
In order to achieve the above purpose, an embodiment of the invention provides a traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver, which is prepared from the following raw materials:
3-6 parts of kudzuvine root, 3-8 parts of hovenia dulcis thunb and 1-5 parts of Tibetan tea;
1 to 3 parts of cogongrass rhizome; 2-5 parts of mulberry and 1-3 parts of liquorice;
0.5 to 2 parts of dried orange peel and 1 to 3 parts of tuckahoe; 1 to 3 parts of medlar;
2 to 5 parts of cardamon, 1 to 3 parts of alisma orientale and 1 to 3 parts of coix seed;
1 to 3 parts of eupatorium; 1 to 3 parts of ginseng; 1 to 3 parts of platycodon grandiflorum;
0.5 to 1 part of madder root and 1 to 5 parts of perilla;
1 to 3 parts of pipewort; 1 to 3 parts of lycopodium clavatum.
In the preferred scheme of the invention, the traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver is prepared from the following raw materials:
4 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 2 parts of Tibetan tea;
2 parts of cogongrass rhizome; 3 parts of mulberry and 2 parts of liquorice;
0.8 parts of dried orange peel and 2 parts of poria cocos; 1 part of medlar;
2 parts of cardamon, 2 parts of alisma orientale and 1.8 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
0.8 part of madder and 2.6 parts of perilla;
2 parts of pipewort; 2 parts of lycopodium clavatum.
In the preferred scheme of the invention, the traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver is prepared from the following raw materials:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of pipewort; 3 parts of lycopodium clavatum.
Based on the traditional Chinese medicine composition disclosed by the invention, the invention also discloses a preparation method of the traditional Chinese medicine composition, which comprises the following steps:
(1) Pretreatment: cutting and pulverizing the Chinese medicinal materials except Tibetan tea to refine the Chinese medicinal materials;
(2) Decocting: soaking the refined Chinese medicinal materials in water for soft swelling, adding Tibetan tea, and decocting under heating; decocting for several times, filtering the decoction after each decoction, mixing decoctions, cooling, and standing for use;
(3) Concentrating: filtering the cooled and standing decoction, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation.
The preparation method of the invention has the following optimization scheme: soaking the thinned Chinese medicine material in water for 1-2 hr, adding Tibetan tea, decocting for 30-60 min for 2-3 times, and merging the filtrates.
The preparation method of the invention has the following optimization scheme: cooling the decoction to normal temperature, refining for 6-8 h, and filtering with a filtering pore diameter of 60-100 meshes.
The preparation method of the invention has the following optimization scheme: the Chinese medicinal preparation comprises paste, pill, granule, capsule and oral liquid.
In summary, the invention has the following advantages:
1. the traditional Chinese medicine composition disclosed by the invention is prepared according to the characteristics among medicines, and the traditional Chinese medicine composition is synergistic mutually, so that the alcohol can be disintoxicated, the inhibition effect of alcohol on the expression of mRNA and protein of alcohol dehydrogenase is lightened, and the enzyme activity concentration of the alcohol dehydrogenase is improved; secondly, the activity of acetaldehyde dehydrogenase can be improved, the accumulation effect of acetaldehyde in the liver can be reduced, and the liver can be protected while the anti-alcohol effect can be improved.
2. The invention can be prepared by adopting a decoction method, and the prepared extract can be converted into various conventional dosage forms, has simple production process, is convenient for simplifying operation procedures and is beneficial to saving production cost.
3. The invention can reduce gastrointestinal reaction and diarrhea symptoms after optimizing the formula of the traditional Chinese medicine composition.
Drawings
FIG. 1 is a diagram showing a liver pathological section of a normal mouse according to an embodiment of the present invention;
FIG. 2 is a diagram of liver pathology in a model group of mice according to one embodiment of the present invention;
FIG. 3 is a diagram showing liver pathological section of mice in example 1 according to one embodiment of the present invention.
Detailed Description
The invention provides a traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver, which is prepared from the following raw materials:
3-6 parts of kudzuvine root, 3-8 parts of hovenia dulcis thunb and 1-5 parts of Tibetan tea;
1 to 3 parts of cogongrass rhizome; 2-5 parts of mulberry and 1-3 parts of liquorice;
0.5 to 2 parts of dried orange peel and 1 to 3 parts of tuckahoe; 1 to 3 parts of medlar;
2 to 5 parts of cardamon, 1 to 3 parts of alisma orientale and 1 to 3 parts of coix seed;
1 to 3 parts of eupatorium; 1 to 3 parts of ginseng; 1 to 3 parts of platycodon grandiflorum;
0.5 to 1 part of madder root and 1 to 5 parts of perilla;
1 to 3 parts of pipewort; 1 to 3 parts of lycopodium clavatum.
The traditional Chinese medicine composition is prepared from the raw materials in weight, and the traditional Chinese medicine is prepared from the traditional Chinese medicine product sold in the market, namely all traditional Chinese medicines can be purchased in the market.
Example 1:
the embodiment of the invention provides a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which is prepared from the following raw materials:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of pipewort; 3 parts of lycopodium clavatum.
The preparation method of the traditional Chinese medicine composition comprises the following steps:
(1) Pretreatment: cutting and pulverizing the Chinese medicinal materials except Tibetan tea to refine the Chinese medicinal materials;
(2) Decocting: soaking the refined Chinese medicinal materials in water for soft swelling, adding Tibetan tea, and decocting under heating; decocting for several times, filtering the decoction after each decoction, mixing decoctions, cooling, and standing for use;
(3) Concentrating: filtering the cooled and standing decoction, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation.
The invention comprises a plurality of embodiments, and the core of the invention is a traditional Chinese medicine composition, the traditional Chinese medicine preparation method adopted by the invention is a decoction method, the method is a conventional preparation method of the traditional Chinese medicine composition, and the process and parameters of the decoction method are optimized according to the characteristics of the traditional Chinese medicine composition. The corresponding product can be obtained by the following method in each embodiment of the invention.
The preparation method of the traditional Chinese medicine composition comprises the following steps:
(1) Pretreatment: the traditional Chinese medicine raw materials except the Tibetan tea are cut and crushed into fine particles or powder, so that the traditional Chinese medicine raw materials are thinned, the thinned raw materials mainly improve the surface area and destroy cell walls, and the traditional Chinese medicine active ingredients are more easy to overflow during decoction.
(2) Decocting: adding the refined traditional Chinese medicine raw materials into water with the weight being 20 times of that of the traditional Chinese medicine raw materials, soaking for 2 hours until the traditional Chinese medicine raw materials are soft, adding Tibetan tea after soaking, and heating and decocting; decocting for 50min, pouring out decoction, adding water, decocting, and repeating the operation. After 3 times of decoction, filtering the decoction after each time of decoction by using an 80-mesh filter screen, then combining the decoctions, cooling and standing for 6 hours for later use.
(3) Concentrating: heating and boiling the cooled and standing decoction, filtering with a filter screen, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation. The Chinese medicinal preparation comprises paste, pill, granule, capsule and oral liquid.
Example 2:
the embodiment of the invention provides a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which is prepared from the following raw materials:
4 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 2 parts of Tibetan tea;
2 parts of cogongrass rhizome; 3 parts of mulberry and 2 parts of liquorice;
0.8 parts of dried orange peel and 2 parts of poria cocos; 1 part of medlar;
2 parts of cardamon, 2 parts of alisma orientale and 1.8 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
0.8 part of madder and 2.6 parts of perilla;
2 parts of pipewort; 2 parts of lycopodium clavatum.
The preparation method of the traditional Chinese medicine composition comprises the following steps:
(1) Pretreatment: cutting and pulverizing the Chinese medicinal materials except Tibetan tea to refine the Chinese medicinal materials;
(2) Decocting: soaking the refined Chinese medicinal materials in water for soft swelling, adding Tibetan tea, and decocting under heating; decocting for several times, filtering the decoction after each decoction, mixing decoctions, cooling, and standing for use;
(3) Concentrating: filtering the cooled and standing decoction, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation.
Example 3:
the embodiment of the invention provides a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which is prepared from the following raw materials:
3 parts of kudzuvine root, 5 parts of hovenia dulcis thunb and 2 parts of Tibetan tea;
2 parts of cogongrass rhizome; 3 parts of mulberry and 2 parts of liquorice;
0.9 parts of dried orange peel and 2 parts of poria cocos; 1 part of medlar;
2 parts of cardamon, 2 parts of alisma orientale and 2 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
0.8 part of madder and 2.5 parts of perilla;
1.6 parts of pipewort; 2 parts of lycopodium clavatum.
The preparation method of the traditional Chinese medicine composition in the embodiment 3 comprises the following steps:
(1) Pretreatment: the traditional Chinese medicine raw materials except the Tibetan tea are cut and crushed into fine particles or powder, so that the traditional Chinese medicine raw materials are thinned, the thinned raw materials mainly improve the surface area and destroy cell walls, and the traditional Chinese medicine active ingredients are more easy to overflow during decoction.
(2) Decocting: adding the refined traditional Chinese medicine raw materials into water with the weight being 20 times of that of the traditional Chinese medicine raw materials, soaking for 2 hours until the traditional Chinese medicine raw materials are soft, adding Tibetan tea after soaking, and heating and decocting; decocting for 50min, pouring out decoction, adding water, decocting, and repeating the operation. After 3 times of decoction, filtering the decoction after each time of decoction by using an 80-mesh filter screen, then combining the decoctions, cooling and standing for 6 hours for later use.
(3) Concentrating: heating and boiling the cooled and standing decoction, filtering with a filter screen, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation. The Chinese medicinal preparation comprises paste, pill, granule, capsule and oral liquid.
Example 4:
the embodiment of the invention provides a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which is prepared from the following raw materials:
5 parts of kudzuvine root, 7 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 5 parts of mulberry and 1 part of liquorice;
1 part of dried orange peel and 2 parts of poria cocos; 3 parts of medlar;
2 parts of cardamon, 1 part of alisma orientale and 1 part of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of pipewort; 3 parts of lycopodium clavatum.
The preparation method of the traditional Chinese medicine composition in the embodiment 4 comprises the following steps:
(1) Pretreatment: the traditional Chinese medicine raw materials except the Tibetan tea are cut and crushed into fine particles or powder, so that the traditional Chinese medicine raw materials are thinned, the thinned raw materials mainly improve the surface area and destroy cell walls, and the traditional Chinese medicine active ingredients are more easy to overflow during decoction.
(2) Decocting: adding the refined traditional Chinese medicine raw materials into water with the weight being 20 times of that of the traditional Chinese medicine raw materials, soaking for 2 hours until the traditional Chinese medicine raw materials are soft, adding Tibetan tea after soaking, and heating and decocting; decocting for 50min, pouring out decoction, adding water, decocting, and repeating the operation. After 3 times of decoction, filtering the decoction after each time of decoction by using an 80-mesh filter screen, then combining the decoctions, cooling and standing for 6 hours for later use.
(3) Concentrating: heating and boiling the cooled and standing decoction, filtering with a filter screen, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation. The Chinese medicinal preparation comprises paste, pill, granule, capsule and oral liquid.
Example 5:
the embodiment of the invention provides a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which is prepared from the following raw materials:
4 parts of kudzuvine root, 3 parts of hovenia dulcis thunb and 5 parts of Tibetan tea;
2 parts of cogongrass rhizome; 3 parts of mulberry and 3 parts of liquorice;
1.2 parts of dried orange peel and 2.2 parts of poria cocos; 1.7 parts of medlar;
2.8 parts of cardamon, 1.6 parts of alisma orientale and 2 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 35 parts of perilla;
3 parts of pipewort; 3 parts of lycopodium clavatum.
The preparation method of the traditional Chinese medicine composition in the embodiment 5 comprises the following steps:
(1) Pretreatment: the traditional Chinese medicine raw materials except the Tibetan tea are cut and crushed into fine particles or powder, so that the traditional Chinese medicine raw materials are thinned, the thinned raw materials mainly improve the surface area and destroy cell walls, and the traditional Chinese medicine active ingredients are more easy to overflow during decoction.
(2) Decocting: adding the refined traditional Chinese medicine raw materials into water with the weight being 20 times of that of the traditional Chinese medicine raw materials, soaking for 2 hours until the traditional Chinese medicine raw materials are soft, adding Tibetan tea after soaking, and heating and decocting; decocting for 50min, pouring out decoction, adding water, decocting, and repeating the operation. After 3 times of decoction, filtering the decoction after each time of decoction by using an 80-mesh filter screen, then combining the decoctions, cooling and standing for 6 hours for later use.
(3) Concentrating: heating and boiling the cooled and standing decoction, filtering with a filter screen, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract.
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation. The Chinese medicinal preparation comprises paste, pill, granule, capsule and oral liquid.
Experimental example one, the anti-hangover and liver-protecting effects of the Chinese medicinal composition of the invention are checked
1. ICR mice were randomly assigned to a blank group, a model group, and a group of example 1 of the present invention, 10 animals per group. In addition to the blank group and the model group, distilled water was administered by gavage every day at 0.2ml/10g of body weight, example 1 of the present invention was administered as the corresponding drug in the group of example 1 at a crude drug concentration of 0.2g/ml, once daily, the gavage dose was 0.2ml/10g of body weight, and the body weight was weighed 1 time every 3 days for continuous administration for 12 days to adjust the administration dose. After 15 days of administration, molding and administration are carried out simultaneously, 56-degree Erguotou white spirit is administered at 9 am, the dosage is 12mL/kg, the administration is continued at 5 night, the molding is continued for 10 days, after the last administration, the blood sample and liver tissue of each group of mice are taken for index detection after 12 hours, and no water is forbidden after the last administration.
2. Serum ALT and AST detection results of mice with acute alcoholic liver injury
Conclusion of experiment:
the serum index of the blank group was significantly lower than that of the model group and the example group, indicating that the mice liver had been damaged after prolonged gastric gavage.
Compared with a blank group, the ALT and AST contents and liver indexes of the embodiment 1 of the invention are higher than those of the model group, but lower than those of the model group, and are between the model group and the blank group; and the data parameters have significant differences; therefore, the pharmaceutical composition of the embodiment 1 of the invention has good protection effect on liver injury after drinking and can reduce liver injury.
ALT and AST are bright phase indexes in liver function detection, wherein the normal value of glutamic-oxaloacetic transaminase AST is 8-40u/L (various liver diseases, early myocardial infarction and liver cirrhosis liver cancer with necrotic liver cells are abnormally high). The normal value of the glutamic-pyruvic transaminase ALT is 5-40u/L (the obstructive jaundice caused by hepatitis drug poisoning and the cholangitis cholangioma caused by hepatic cirrhosis have abnormal high). Liver index (%) =liver weight (g)/body weight (g) ×100%, which is a main appearance index of an animal model of alcoholic liver injury, and liver index increase generally represents liver weight increase and occurrence of hepatocyte edema.
3. Acute alcoholic liver injury mouse liver pathology experiment
The liver of the mice in 3 groups were HE-stained to observe pathological sections by selecting the blank group, model group and example 1 group according to the present invention, and the observation results are shown in the figure.
Observation conclusion:
(1) Blank group: referring to fig. 1, the liver lobule has clear structure, the hepatic cell chordae are orderly arranged, the liver sinus is normal, the nuclear structure is clear, and no pathological change exists.
(2) Model group: referring to fig. 2, the hepatic cell cord disorder, the hepatic cell swelling, the hepatic sinus stenosis, the cytoplasmic loosening, the variable size and the variable quantity of fatty vacuoles and water sample denaturation are visible in cytoplasm, a plurality of punctate or focal necrosis occurs in lobules, and a great amount of inflammatory cell infiltration exists in a collecting duct region.
(3) Example 1: referring to fig. 3, the pathological damage of the liver cells of the mice is improved, the swelling of the liver cells is reduced, the fat vacuoles in cytoplasm are reduced, the denaturation of water samples is obviously reduced, and the inflammatory cell infiltration in liver lobules is obviously improved.
The pathological results show that: the model composition is successful, and the medicine has the improvement effects on liver cell swelling, inflammatory cell infiltration and the like, and the medicine has the liver protection effect.
4. Example 1 Effect of the invention on alcohol dehydrogenase and acetaldehyde dehydrogenase
The alcohol dehydrogenase and the acetaldehyde dehydrogenase of the invention can be quantitatively detected by spectrophotometry in the prior art; the specific method is as follows:
(1) Self-contained instruments and supplies:
ultraviolet spectrophotometers, table centrifuges, water baths, 1mL quartz cuvettes, adjustable pipette guns, mortar/homogenizer, ice and distilled water.
(2) Extracting tissue fluid according to volume (mL) 1: 5-10, homogenizing in ice bath, centrifuging at 4 deg.c for 20min, and setting the supernatant on ice to be measured. Preheating an ultraviolet spectrophotometer for more than 30min, adjusting the wavelength to 340nm, and zeroing distilled water; the reagent was preheated for 15min.
(3) Reagents are respectively added into A1 mL quartz cuvette, after the reagents are fully mixed, the absorbance A1 at the position of 340nm is measured for 30s, the quartz cuvette is quickly placed in a water bath at 37 ℃ for 1min, the absorbance A2 at the position of 90s is quickly wiped out, the measurement of delta A measuring tube = A2 measuring-A1, the measurement of delta A blank tube = A2 blank-A1 blank, and the measurement of delta A = delta A measuring tube-delta A blank tube are calculated.
(4) Detection of enzyme Activity in different groups
Definition of enzyme activity: the generation of 1nmol of NADH per minute per mL of sample is defined as 1 enzyme activity unit.
ALDH enzyme Activity (U/mL) =ΔA ∈xd ×109×V inverse total ≡V sample ≡T
Wherein ε: NADH molar extinction coefficient, 6.22X103L/mol/cm; d: cuvette optical path, 1cm; v inverse total: 0.001L of total volume of the reaction system; v sample: sample volume in reaction system, 0.2mL; sample V total: the volume of the extracting solution is 1mL; cpr: the concentration of the sample protein, mg/mL, needs to be determined by oneself; w: sample mass, g; t: reaction time: 1min; .
The ethanol dehydrogenase and acetaldehyde dehydrogenase detection results of the present invention are shown in the following table:
| group of | Ethanol dehydrogenase u/mg | Acetaldehyde dehydrogenase u/mg |
| Blank group | 3.75±1.15 | 1.56±0.68 |
| Model group | 1.38±0.72 | 0.68±0.45 |
| Example 1 | 3.15±1.04 | 3.15±0.92 |
Conclusion of experiment:
(1) From the above table, it can be seen that after the mice are successfully modeled, the model group does not take any anti-hangover and liver-protecting drugs, the liver is damaged, the expression of the protein of alcohol dehydrogenase and acetaldehyde dehydrogenase of the model group is limited, the activity is the lowest, and the liver metabolism of the model group is affected, which is consistent with the experimental conclusion in document 5.
(2) Compared with the blank group alcohol dehydrogenase and the acetaldehyde dehydrogenase, the activity of the example 1 is improved, but the amplification of the acetaldehyde dehydrogenase is larger, which proves that the pharmaceutical composition of the invention can remarkably improve the expression of the acetaldehyde dehydrogenase in the liver, so that the reactivity of the acetaldehyde dehydrogenase is improved, and more substrate acetaldehyde can be converted in unit time; the protection of the liver is more beneficial.
Experimental example II, test for anti-hangover effect of the drug in example 1 of the present invention
Mice weighing 20+ -2 g were selected and randomly divided into a blank group, a group of the invention of example 1, a group of Pueraria lobata decoction, and 10 mice each. After mice fasted for 6 hours, the mice in the blank group are filled with gastric distilled water, the other groups are respectively filled with corresponding tested medicines, the gastric dosage is 0.2mL/10g of body weight, each group is respectively filled with 56 DEG Erguotor 15mL/kg after 30 minutes, then gait conditions of the mice are observed and recorded, the disappearance of the eversion front (drunk) and the recovery time (sober-up) are recorded, and the drunk latency (the time from the gastric filling Erguotor to the complete disappearance of the eversion front) and the drunk duration (the time from the complete disappearance of the eversion front to the complete recovery) are calculated; the test results are shown in the following table:
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experimental example III, influence of Chinese medicinal composition on expression of mouse alcohol dehydrogenase and acetaldehyde dehydrogenase under different conditions
(1) The effect of a pair of alcohol dehydrogenase and acetaldehyde dehydrogenase in combination with the experimental examples of the present invention can be found: compared to the model group and the blank group, the blank group was not dosed with the drug, and the model group showed liver damage after modeling, and the total amount of enzyme activities of alcohol dehydrogenase and acetaldehyde dehydrogenase was increased from the data, but the alcohol dehydrogenase in example 1 was lower than the blank group, and the acetaldehyde dehydrogenase in example 1 was higher than the blank group. It is known from the reference 5 that alcohol can inhibit the expression of alcohol dehydrogenase and acetaldehyde dehydrogenase by the liver, and therefore it is necessary to verify whether the composition of example 1 of the present invention directly increases the expression of acetaldehyde dehydrogenase or decreases the inhibitory effect of alcohol on the production of alcohol dehydrogenase by the liver.
The embodiment of the invention provides a traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver, which is prepared from the following raw materials:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of pipewort; 3 parts of lycopodium clavatum; the composition was the same as in example 1.
(2) ICR mice were randomly assigned to a blank group a, a blank group B, and a group of example 1 according to the present invention, 10 mice per group. The blank A was given 0.2ml/10g of distilled water per day by gavage, the blank B and the example 1 were given the example 1 of the present invention as the respective drugs at the concentration of 0.2g/ml crude drug, once per day, the gavage dose was 0.2ml/10g of body weight, and the body weight was weighed 1 time every 3 days for 12 days continuously to adjust the dose. After 15 days of administration, molding and administration were performed simultaneously, and the mice of example 1 were molded, and the mice of the blank group B were not molded, and were subjected to normal metering of the gastric lavage drug. The mice of example 1 were given 56 ° Erguotou white spirit at 9 am every day, the dose was 12mL/kg, the administration was continued at 5 pm, the molding was continued for 10 days, after the last administration, no water was forbidden in the fasting, and after 12 hours, blood samples and liver tissues of each group of mice were taken for index detection. The blank group a and the example group in the third experimental example were treated in the same manner as the blank group of the first experimental example and the example 1 group.
The results of the ethanol dehydrogenase and acetaldehyde dehydrogenase assays of experimental example three of the present invention are shown in the following table:
| group of | Ethanol dehydrogenase u/mg | Acetaldehyde dehydrogenase u/mg |
| Blank group A | 3.75±1.15 | 1.56±0.68 |
| Blank B | 3.82±1.17 | 4.25±1.34 |
| Experimental example one model set | 1.38±0.72 | 0.68±0.45 |
| Example 1 | 3.15±1.04 | 3.15±0.92 |
In the third experimental example, a blank control group B is actually added on the basis of the first experimental example, the mice in the blank control group B are normal mice, and the blank control group B is actually that the normal mice are directly filled with the drug in the example 1 so as to test the expression effect of the drug on liver enzymes. From the expression results, the normal mice in blank group B were not able to increase the effect of alcohol dehydrogenase in normal mice after the gastric lavage, but it can be seen that the mice after molding (administration of alcohol) were indeed reduced compared to the comparison of example 1 and model group, which can result in that the traditional Chinese medicine composition of the present invention was not able to directly increase the expression of enzyme in normal mice, but was able to reduce the negative effect of alcohol on the expression of alcohol dehydrogenase in liver.
Also, it can be seen that the components of the present invention can promote expression of acetaldehyde dehydrogenase in the liver of mice, both in model mice (example 1 vs model group) and in normal mice (comparison of blank A and blank B).
Experimental example four, influence of different Chinese medicinal compositions on ethanol dehydrogenase and acetaldehyde dehydrogenase of model-building mice
1. Preparing a plurality of groups of traditional Chinese medicine compositions in different experimental examples, wherein the traditional Chinese medicine compositions in different experimental examples are different in composition, and the contents of the components are kept similar; the composition of each experimental example is specifically shown below.
Experimental example 41:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; and 2 parts of platycodon grandiflorum.
Experimental example 42:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla.
Experimental example 42 is based on experimental example 41, 1 part of radix Rubiae and 2 parts of Perillae herba are added.
Experimental example 43 (same as example 1):
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of pipewort; 3 parts of lycopodium clavatum.
Experiment example 43 is obtained by adding 1 part of radix Rubiae and 2 parts of Perillae herba on the basis of experiment example 41; 2 parts of pipewort; 3 parts of lycopodium clavatum.
Experimental example 44:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of selfheal; 3 parts of pine knots.
Experimental example 44 is to use Spica Prunellae instead of flos Eriocauli and lignum Pini nodi instead of herba Lycopodii.
Experimental example 45:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of semen cassiae; and 3 parts of kadsura pepper stem.
Experimental example 45 is to replace flos Eriocauli with semen Cassiae and herba Piperis Futokadsurae with herba Lycopodii.
Experimental example 46:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of buddleja officinalis; 3 parts of gentiana macrophylla.
Experimental example 46 is to replace flos Eriocauli with flos Buddlejae and herba Lycopodii with radix Gentianae Marcrophyllae.
Experimental example 47:
5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb and 3 parts of Tibetan tea;
2 parts of cogongrass rhizome; 4 parts of mulberry and 3 parts of liquorice;
1 part of dried orange peel and 3 parts of poria cocos; 2.5 parts of medlar;
2.5 parts of cardamon, 1.6 parts of alisma orientale and 1.9 parts of coix seed;
2 parts of eupatorium; 1 part of ginseng; 2 parts of platycodon grandiflorum;
1 part of madder and 2 parts of perilla;
2 parts of feather cockscomb seed; 3 parts of gentiana macrophylla.
Experimental example 47 is to replace flos Eriocauli with semen Celosiae and herba Lycopodii with radix Angelicae Pubescentis.
2. The corresponding Chinese medicinal extracts were prepared according to the method shown in example 1 from the Chinese medicaments in experimental examples 21 to 27, and then the contents of alcohol dehydrogenase and acetaldehyde dehydrogenase of mice were measured according to the method of experimental example one or the method of experimental example three.
The pipewort of the invention has the effects of dispelling wind and dissipating heat, improving eyesight and removing nebula. Treating nebula, sparrow, headache, odontalgia, pharyngitis, epistaxis; in the prior art, the pipewort has partial similar efficacy with the selfheal, the cassia seed, the buddleja officinalis, the feather cockscomb seed and the like, so the invention is verified by using the substances. In the same way, the lycopodium clavatum has the effects of dispelling wind and removing dampness and relaxing tendons and activating collaterals; in the prior art, the traditional Chinese medicine has partial similar efficacy with pine knots, kadsura pepper stems, large-leaved gentian and radix angelicae pubescentis, so the invention is verified by using the substances.
The experimental results of experimental example four of the present invention are shown in the following table:
| group of | Ethanol dehydrogenase u/mg | Acetaldehyde dehydrogenase u/mg |
| Blank group | 3.75±1.15 | 1.56±0.68 |
| Building module | 1.38±0.72 | 0.68±0.45 |
| Experimental example 41 | 1.58±0.86 | 1.54±0.58 |
| Experimental example 42 | 1.64±0.75 | 1.50±0.49 |
| Experimental example 43 | 3.15±1.04 | 3.15±0.92 |
| Experimental example 44 | 1.72±0.95 | 1.46±0.46 |
| Experimental example 45 | 1.62±0.93 | 1.47±0.53 |
| Experimental example 46 | 1.63±0.82 | 1.62±0.79 |
| Experimental example 47 | 1.83±0.91 | 1.45±0.67 |
From the above, it can be seen that in experimental examples 44-48, the replacement of the eriocaulon henryi with other traditional Chinese medicines was performed, and the replacement of the eriocaulon henryi with other traditional Chinese medicine components was performed, and the effect after the replacement was not significantly improved. Compared with the experimental example 42 and the modeling module, the experimental example 41 has smaller difference of the activity concentration of the alcohol dehydrogenase and a certain increase of the acetaldehyde dehydrogenase; but still to a much lesser extent than the components of experimental example 43 or example 1 of the present invention; through component comparison, the invention has remarkable influence on alcohol dehydrogenase and acetaldehyde dehydrogenase after adding the eriocaulon and the lycopodium clavatum, and particularly has larger influence on the acetaldehyde dehydrogenase.
Claims (7)
1. The traditional Chinese medicine composition for dispelling the effects of alcohol and protecting liver is characterized by comprising the following raw materials:
3-6 parts of kudzuvine root, 3-8 parts of hovenia dulcis thunb, 1-5 parts of Tibetan tea,
1 to 3 parts of cogongrass rhizome, 2 to 5 parts of mulberry, 1 to 3 parts of liquorice,
0.5 to 2 parts of dried orange peel, 1 to 3 parts of tuckahoe, 1 to 3 parts of medlar,
2 to 5 parts of cardamon, 1 to 3 parts of alisma orientale, 1 to 3 parts of coix seed,
1 to 3 parts of eupatorium, 1 to 3 parts of ginseng, 1 to 3 parts of platycodon grandiflorum,
0.5 to 1 part of madder, 1 to 5 parts of perilla,
1-3 parts of pipewort and 1-3 parts of lycopodium clavatum.
2. The traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver as claimed in claim 1, which is characterized by comprising the following raw materials: 4 parts of kudzuvine root, 6 parts of hovenia dulcis thunb, 2 parts of Tibetan tea,
2 parts of cogongrass rhizome, 3 parts of mulberry, 2 parts of liquorice,
0.8 part of dried orange peel, 2 parts of tuckahoe, 1 part of medlar,
2 parts of cardamon, 2 parts of alisma, 1.8 parts of coix seed,
2 parts of eupatorium, 1 part of ginseng, 2 parts of platycodon root,
0.8 part of madder, 2.6 parts of perilla,
2 parts of pipewort and 2 parts of lycopodium clavatum.
3. The traditional Chinese medicine composition for dispelling effects of alcohol and protecting liver as claimed in claim 1, which is characterized by comprising the following raw materials: 5 parts of kudzuvine root, 6 parts of hovenia dulcis thunb, 3 parts of Tibetan tea,
2 parts of cogongrass rhizome, 4 parts of mulberry, 3 parts of liquorice,
1 part of dried orange peel, 3 parts of tuckahoe, 2.5 parts of medlar,
2.5 parts of cardamon, 1.6 parts of alisma orientale, 1.9 parts of coix seed,
2 parts of eupatorium, 1 part of ginseng, 2 parts of platycodon root,
1 part of madder, 2 parts of perilla,
2 parts of pipewort and 3 parts of lycopodium clavatum.
4. The preparation method of the traditional Chinese medicine composition according to any one of claims 1-3, comprising the following steps:
(1) Pretreatment: cutting and pulverizing the Chinese medicinal materials except Tibetan tea to refine the Chinese medicinal materials;
(2) Decocting: soaking the refined Chinese medicinal materials in water for soft swelling, adding Tibetan tea, and decocting under heating; decocting for several times, filtering the decoction after each decoction, mixing decoctions, cooling, and standing for use;
(3) Concentrating: filtering the cooled and standing decoction, evaporating and concentrating with slow fire after filtering, and concentrating to obtain extract;
(4) Preparation: adding adjuvants into the extract, and making into Chinese medicinal preparation.
5. The method of manufacturing according to claim 4, wherein: adding the refined traditional Chinese medicine raw materials into water, soaking for 1-2 h, adding Tibetan tea, decocting for 30-60 min each time, decocting for 2-3 times, and combining the filtrates.
6. The method of manufacturing according to claim 4, wherein: cooling the decoction to normal temperature, standing for 6-8 hours, and filtering, wherein the filtering aperture is 60-100 meshes.
7. The method of manufacturing according to claim 4, wherein: the Chinese medicinal preparation comprises paste, pill, granule, capsule and oral liquid.
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| CN1108553A (en) * | 1994-03-17 | 1995-09-20 | 李玉芹 | Product for dispelling the effects of alcohol |
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| CN111481646A (en) * | 2020-04-10 | 2020-08-04 | 雅安职业技术学院 | Medicinal composition for dispelling effects of alcohol and protecting liver, preparation method and application |
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| CN1108553A (en) * | 1994-03-17 | 1995-09-20 | 李玉芹 | Product for dispelling the effects of alcohol |
| CN1113801A (en) * | 1994-05-11 | 1995-12-27 | 温贵明 | health medicine for preventing alcoholic intoxication and relieving acute alcoholism |
| CN1134282A (en) * | 1995-04-27 | 1996-10-30 | 贾毅 | Tea for sobering up |
| CN109999165A (en) * | 2018-11-09 | 2019-07-12 | 重庆涪陵李志沧中医骨伤医院有限公司 | One kind is sobered up Chinese medicine composition and its preparation and application |
| CN111481646A (en) * | 2020-04-10 | 2020-08-04 | 雅安职业技术学院 | Medicinal composition for dispelling effects of alcohol and protecting liver, preparation method and application |
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