CN115844955A

CN115844955A - Chinese veterinary drug 'Tekang' compound preparation and preparation method and application thereof

Info

Publication number: CN115844955A
Application number: CN202211727376.3A
Authority: CN
Inventors: 陈伟; 陈阳; 吴润; 金彦西; 郑涛
Original assignee: Southwest Jiaotong University
Current assignee: Southwest Jiaotong University
Priority date: 2022-12-31
Filing date: 2022-12-31
Publication date: 2023-03-28

Abstract

The invention belongs to the technical field of traditional Chinese veterinary medicines, and particularly relates to a traditional Chinese veterinary medicine 'tianzhi' compound preparation as well as a preparation method and application thereof. The traditional Chinese veterinary medicine 'Tekang' compound preparation is prepared from the following raw materials in parts by weight: 10-20 parts of lonicera confusa extract, 20-40 parts of macleaya cordata extract, 10-20 parts of scutellaria baicalensis extract, 10-20 parts of astragalus membranaceus extract, 5-20 parts of desmodium extract and 5-20 parts of cassia twig extract. The veterinary drug 'tiantikang' compound preparation provided by the invention can effectively prevent common pathogenic bacteria in the chicken breeding process: escherichia coli, staphylococcus aureus, salmonella, and pasteurella. Has important research value in the field of veterinary drugs, improves the level of 'green' cultivation in China and ensures the food safety.

Description

Chinese veterinary drug 'Tekang' compound preparation and preparation method and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese veterinary medicines, and particularly relates to a veterinary medicine 'tianzhi' compound preparation as well as a preparation method and application thereof.

Background

Since ancient times, chickens have taken an important position in the dietary structure of humans, not only providing high quality protein, but also being rich in lysine and threonine. The chicken breeding is the most active growth point and the main supporting industry in rural economy in China and is an important component of animal husbandry. However, in the process of chicken breeding, the chicken is easily attacked by pathogenic microorganisms such as escherichia coli, staphylococcus aureus, salmonella, pasteurella, rotavirus and the like, so that diseases such as coccidiosis, bronchitis, red and white dysentery, proventriculitis and the like are caused, and the chicken grows slowly and even dies. Particularly, the method is accompanied with the change of the free-ranging mode of small-scale and large-population in the chicken industry in China to intensive and organized scale breeding, and brings huge damage and disasters to the breeding industry.

Antibiotics are often added to chicken feed to prevent disease. The use of the antibiotic feed additive greatly improves the production efficiency of chicken and eggs and promotes the high-speed development of chicken breeding industry. However, driven by interest, more and more antibiotics are used in chicken farming and are gradually evolving to be unjustified or even abused. Antibiotics become a major problem in the global public health field, not only pollute the environment and harm human health, but also cause bacteria to generate drug resistance and the like. In order to promote the green sustainable development of the breeding industry in China, the food safety is practically guaranteed, and the addition of antibiotics is completely forbidden. Therefore, under the condition of 'banning resistance', how to keep the continuous and efficient production of the breeding industry needs to be solved urgently to find a high-efficiency and safe 'substitute resistance' product.

Disclosure of Invention

In order to solve the shortage and the deficiency of the existing 'anti' -product and develop and create a green and safe 'anti' -compound preparation of a traditional Chinese veterinary medicine, the invention aims to provide a 'anti' -compound preparation of a traditional Chinese veterinary medicine and a preparation method and application thereof.

The invention provides the following technical scheme: a veterinary drug 'Tekang' compound preparation comprises 10-20 parts of lonicera confusa extract, 20-40 parts of macleaya cordata extract, 10-20 parts of scutellaria baicalensis extract, 10-20 parts of astragalus membranaceus extract, 5-20 parts of desmodium extract and 5-20 parts of cassia twig extract.

Preferably, the composition is prepared from the following raw materials in parts by weight: 15 parts of lonicera confusa extract, 35 parts of macleaya cordata extract, 15 parts of scutellaria baicalensis extract, 15 parts of astragalus membranaceus extract, 10 parts of desmodium extract and 10 parts of cassia twig extract.

The invention also aims to provide a preparation method of the traditional Chinese medicine compound preparation, which comprises the following steps: extracting the effective components of the Chinese medicinal materials with water and/or ethanol as extraction solvent by one or more of water extraction, acid extraction and alkali precipitation, ultrasonic-assisted extraction, and water extraction and alcohol precipitation, concentrating, and drying to obtain the Chinese medicinal extract.

Preferably, the water extraction method comprises the steps of adding 5-10L of water into 1kg of medicinal materials, soaking for 1-2 h, heating and refluxing for extraction for 1-3 times, each time for 1-3 h, combining filtrates, concentrating and drying to obtain the extract.

Preferably, the acid-base precipitation method comprises the steps of adding 1kg of medicinal materials into 5-10L of dilute hydrochloric acid with the pH value of 1-3, soaking for 1-2 h, heating, refluxing and extracting for 1-3 times, wherein each time is 1-3 h, adding NaOH solution with the pH value of 12-14 to adjust the pH value to 10-12 when the extracting solution is concentrated to 10-30% of the initial volume, precipitating, carrying out suction filtration to collect solids, and drying to obtain the extract.

Preferably, the ultrasonic-assisted extraction method is that 3 to 8L of ethanol is added into 1kg of medicinal materials, and ultrasonic extraction is carried out for 1 to 3 times at the power of 25 to 55kHz and the temperature of 20 to 60 ℃, and each time lasts for 0.5 to 1.0 hour; concentrating the obtained extracting solution, standing for 10-24 h, centrifuging at 5000-10000 rpm, taking supernatant, concentrating and drying to obtain the extract.

Preferably, the water extraction and alcohol extraction method comprises the steps of adding 5-10L of water into 1kg of medicinal materials, soaking for 1-2 h, heating, refluxing and extracting for 1-3 times, wherein each time is 1-3 h, adding absolute ethyl alcohol until the alcohol content is 80-90% when the extracting solution is concentrated to 10-30% of the initial volume, standing for 10-24 h at the temperature of 2-8 ℃, precipitating, carrying out suction filtration to collect solids, and drying the extract.

The improvement of the veterinary drug 'Tekang' compound preparation is that the selected traditional Chinese medicine components are macleaya cordata extract, scutellaria baicalensis extract, astragalus mongholicus extract, desmodium extract and cassia twig extract.

The preparation method of the veterinary drug compound preparation is further improved in that one or more methods of a water extraction method, an acid-extraction-alkali precipitation method, an ultrasonic-assisted extraction method and a water extraction-alcohol precipitation method are adopted to extract the effective components of the traditional Chinese medicinal materials.

The invention further provides application of the traditional Chinese medicine extract in preparing a veterinary medicine compound preparation, wherein the compound traditional Chinese medicine 'tianti' compound preparation is used for preventing and treating common pathogenic bacteria in chicken breeding: escherichia coli, staphylococcus aureus, salmonella, and Pasteurella to improve chicken breeding efficiency.

Due to the adoption of the technical scheme, the invention has the following beneficial effects: (1) The traditional Chinese medicine components of the traditional Chinese veterinary medicine compound preparation are reasonable in compatibility, the effective components are clear, the system is stable, and the product quality is guaranteed. (2) The preparation method of the traditional Chinese medicine compound preparation is simple to operate, the technology is mature and stable, and reagents used in the whole process are safe and have no toxic or side effect. (3) The Chinese medicinal compound preparation is a pure Chinese medicinal extract, does not contain chemical medicaments and antibiotics, and is non-toxic and free from medicament residues. (4) The traditional Chinese medicinal materials applied in the invention are common in the market, are low in price and easy to obtain, have low cost and can be widely popularized and used. (5) The veterinary drug 'tianti' compound preparation has the effects of resisting bacteria and diminishing inflammation, exerts the effects similar to antibiotics, can effectively prevent common pathogenic bacteria in chicken breeding, improves the breeding efficiency of livestock and poultry, ensures food safety and the like.

These and other objects, features and advantages of the present invention will become more fully apparent from the following detailed description and appended claims, and may be realized by means of the techniques, procedures, and combinations particularly pointed out in the appended claims.

Detailed Description

Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by manufacturers, and are all conventional products available on the market.

The invention relates to a veterinary drug 'tianzhi' compound preparation, which is characterized by comprising the following substances in parts by weight: 10-20 parts of lonicera confusa extract, 20-40 parts of macleaya cordata extract, 10-20 parts of scutellaria baicalensis extract, 10-20 parts of astragalus membranaceus extract, 5-20 parts of desmodium extract and 5-20 parts of cassia twig extract.

Lonicera confusa (Lonicera hypoglauca) is a dried bud or a flower with initial blossom of Lonicera macranthoides, lonicera hypoglauca, lonicera confusa or Lonicera fulvescens belonging to Caprifoliaceae, and is mainly distributed in Sichuan, guizhou, guangdong, guangxi, etc. It is recorded in the miscellaneous records of famous physicians, its taste is sweet, warm and non-toxic, mainly indicated for cold and heat, swollen body, and is listed as the top grade. 2020, recorded in Chinese pharmacopoeia, mountain honeysuckle flower has effects of clearing heat, detoxicating, dispelling wind and dissipating heat, and is mainly used for treating carbuncle swelling and sore, erysipelas, wind-heat type common cold, pyretic toxicity bloody dysentery, etc. The lonicera confusa contains various bioactive substances such as organic acids, flavonoids, saponins, volatile oils and the like, wherein the organic acids have remarkable bioactivity, are considered as main active ingredients of plant-derived medicaments and are the basis for clearing heat and removing toxicity of the lonicera confusa.

Macleaya cordata (willld) r.br.) is commonly known as "earlap", haotong pole, shangchun, etc., is a perennial herb of macleaya genus of papaveraceae family, and is widely distributed in the middle and lower reaches of the Yangtze river and north of south ridge in China. The compendium of materia medica is recorded in: 'Macleaya cordata, raw Jiangnan valley' \8230thatwhen folded, people die immediately and cannot enter the mouth. Has antiinflammatory, antibacterial, and pesticidal effects. The main active component of the compound is alkaloid, and representative compounds comprise sanguinarine, chelerythrine, protopine, allocryptopine and the like.

Scutellaria baicalensis Georgi (Scutellaria baicalensis Georgi), also known as root of Camellia japonica, root of Thalictrum japonicum, is a perennial herb of the genus Scutellaria of the family Labiatae. The Chinese medicinal composition is recorded in Shennong Bencao Jing for the first time, is bitter in taste and cold in nature, and belongs to the lung channel and the gallbladder channel, and clinical practices prove that the Chinese medicinal composition has multiple effects of resisting bacteria, inflammation and viruses, resisting oxidation, reducing blood fat, protecting liver and the like, is called as a Chinese medicinal 'antibiotic', and is also widely applied to veterinary medicine products. The scutellaria baicalensis contains flavonoid, polysaccharide, volatile oil and other substances, and the most main effective component of the scutellaria baicalensis is a flavonoid compound.

Astragalus (Astragalus membranaceus (fisch.) bungee) is a dried root of the leguminous plant Astragalus membranaceus capsular or Astragalus mongholicus, and was originally described in "shennong herbal" in: "mainly abscess deep-rooted carbuncle, long-term decay and wound, pus discharge and pain relief, wind and scab disease". Radix astragali has sweet taste and mild nature, enters spleen and lung meridians, has the effects of invigorating qi and invigorating yang, inducing diuresis to alleviate edema, benefiting defense and consolidating exterior, is mainly used for treating diseases such as spleen and stomach qi deficiency, exterior deficiency and spontaneous perspiration, chronic ulcer and unhealing, and has the effects of tonifying deficiency and assisting qi, expelling toxin, consolidating exterior and the like. The main effective components of radix astragali include saponins, polysaccharides and flavonoids.

Lysimachia christinae Hance is dry whole plant of primula family plant Lysimachia christinae Hance, cold in nature, sweet and salty in flavor, and enters liver, gallbladder, kidney and bladder meridians. Has the effects of clearing heat, promoting diuresis, treating stranguria, clearing liver and gallbladder damp-heat, and clearing heat and detoxicating, and is mainly used for treating heat stranguria, stranguria with stone, liver and gallbladder calculus, jaundice, toxic heat, carbuncle and swelling, etc. The main components are kaempferol and quercetin, and the product has pharmacological effects of promoting bile secretion and excretion, promoting urination and removing urinary calculus, promoting bile secretion and excretion, resisting inflammation, and resisting bacteria.

Ramulus Cinnamomi (Cinnamomum cassia Presl) is dry twig of Cinnamomum cassia Presl of Lauraceae, and has warm nature, pungent and sweet taste, and meridian tropism for heart, lung and bladder. Has effects of inducing sweat, relieving exterior syndrome, warming channels and activating yang, and can be used for treating wind-cold exterior deficiency syndrome, wind-cold exterior excess syndrome, and wind-cold-dampness arthralgia syndrome. The main ingredients are cassia oil, cinnamaldehyde and the like, and the pharmaceutical composition has the pharmacological effects of relieving fever and sweating, tranquilizing and resisting convulsion, invigorating stomach and the like.

The veterinary drug 'substitution-resistant' product is prepared from natural plants, is based on the 'prevention and treatment' concept of the traditional Chinese medicine, is subjected to animal production practice research for thousands of years, is prepared according to the drug state and the compatibility principle, and plays the integral synergistic effect. Moreover, the Chinese herbal medicine contains growth regulating factors, is easy to transform and absorb in animal bodies, and has no toxic residues. Moreover, the natural Chinese herbal medicine has unique antibacterial action mechanism, not only eliminates pathogens through the effective antibacterial components of the Chinese herbal medicine, but also promotes the immune cells of the organism to play a role, and enhances the immune function, thereby achieving the antibacterial action.

According to the traditional Chinese medicine, bacterial infection and virus invasion are generally called as poisoning, the focus is mostly in the lung, the disease is usually manifested as excessive phlegm, fever and excessive internal heat, and the traditional Chinese medicines for clearing heat and removing toxicity, cultivating vitality and tonifying qi are usually used for treating the disease. The Chinese herbal medicine 'substitution' product of the invention refers to the classical book of traditional Chinese medicines, and the compatibility principle of monarch, minister, assistant and guide is applied to prepare the Chinese veterinary medicine compound preparation with the effects of resisting bacteria and diminishing inflammation, improving the immunity of the organism, promoting the growth and the like, thereby improving the breeding efficiency of livestock and poultry and ensuring the food safety.

The preparation method of the traditional Chinese veterinary medicine composition comprises the following steps:

removing impurities from the above 6 medicinal materials, respectively, and pulverizing into coarse powder with particle size of about 0.4 cm.

Extracting the effective component alkaloid in macleaya cordata by using an acid-extraction-alkali precipitation method, taking 1kg of medicinal materials, adding 5-10L of dilute hydrochloric acid with the pH value of 1-3, soaking for 1-2 h, then heating and refluxing for extraction for 1-3 times, wherein each time lasts for 1-3 h, adding NaOH solution with the pH value of 12-14 to adjust the pH value to 10-12 when the extracting solution is concentrated to 10-30% of the initial volume, precipitating, carrying out suction filtration to collect solid, and drying to obtain the macleaya cordata extract.

Extracting honeysuckle and scutellaria baicalensis by using an ultrasonic-assisted extraction method, adding 3-8L of ethanol into 1kg of medicinal materials, and ultrasonically extracting for 1-3 times at the power of 25-55 kHz and the temperature of 20-60 ℃ for 0.5-1.0 h each time; concentrating the obtained extracting solution, standing for 10-24 h, centrifuging at 5000-10000 rpm, taking supernatant, concentrating and drying to obtain the extract.

Extracting astragalus membranaceus by a water extraction and alcohol precipitation method, adding 5-10L of water into 1kg of medicinal materials, soaking for 1-2 hours, heating, refluxing and extracting for 1-3 times, wherein each time is 1-3 hours, concentrating an extracting solution to 10-30% of the initial volume, adding absolute ethyl alcohol until the alcohol content is 80-90%, standing for 10-24 hours at 2-8 ℃, precipitating precipitates, performing suction filtration, collecting solids, and drying the extract.

Extracting the desmodium and the cassia twig by a water extraction method, adding 5-10L of water into 1kg of medicinal materials, soaking for 1-2 h, heating and refluxing for extraction for 1-3 times, each time for 1-3 h, combining filtrates, concentrating and drying to obtain the extract.

The present invention will be further explained with reference to specific examples and comparative examples.

Example 1

A veterinary drug, TIKANG, compound preparation, comprises (by weight parts) flos Lonicerae extract 10, herba Macleayae Cordatae extract 20, scutellariae radix extract 10, radix astragali extract 10, herba Lysimachiae Christinae extract 5, and ramulus Cinnamomi extract 5. Pulverizing the Chinese medicinal extract mixture, stirring, adding adjuvants, packaging, and sterilizing.

Example 2

A veterinary drug, TIKANG, compound preparation, comprises (by weight parts) flos Lonicerae extract 15, herba Macleayae Cordatae extract 25, scutellariae radix extract 15, radix astragali extract 15, herba Lysimachiae Christinae extract 10, and ramulus Cinnamomi extract 10. Pulverizing the Chinese medicinal extract mixture, stirring, adding adjuvants, packaging, and sterilizing.

Embodiment 3

A veterinary drug, TIKANG, compound preparation, comprises (by weight parts) flos Lonicerae extract 20, herba Macleayae Cordatae extract 30, scutellariae radix extract 15, radix astragali extract 15, herba Lysimachiae Christinae extract 15, and ramulus Cinnamomi extract 15. Pulverizing the Chinese medicinal extract mixture, stirring, adding adjuvants, packaging, and sterilizing.

Example 4

A veterinary drug, TIKANG, compound preparation, comprises 15 parts by weight of flos Lonicerae extract, 35 parts by weight of herba Macleayae Cordatae extract, 15 parts by weight of Scutellariae radix extract, 15 parts by weight of radix astragali extract, 10 parts by weight of herba Lysimachiae Christinae extract, and 10 parts by weight of ramulus Cinnamomi extract. Pulverizing the Chinese medicinal extract mixture, stirring, adding adjuvants, packaging, and sterilizing.

Example 5

A veterinary drug, TIKANG, compound preparation, comprises (by weight parts) flos Lonicerae extract 20, herba Macleayae Cordatae extract 40, scutellariae radix extract 20, radix astragali extract 20, herba Lysimachiae Christinae extract 20, and ramulus Cinnamomi extract 20. Pulverizing the Chinese medicinal extract mixture, stirring, adding adjuvants, packaging, and sterilizing.

Comparative example 1

The macleaya cordata powder serving as a traditional Chinese veterinary medicine 'tiaozhi' product sold in the market is selected as a contrast agent.

The effect of the compound preparation is compared and tested.

Selecting common pathogenic bacteria in chicken breeding: escherichia coli (CMCC 44102), staphylococcus aureus (CMCC 26003), salmonella (CMCC 50041) and Pasteurella (CVCC 393) are used as test objects, and the in vitro bacteriostatic activity of each Chinese medicinal extract is screened by using a plate method. The main operation steps are as follows:

preparation of a liquid medicine: dissolving the effective components of the traditional Chinese medicine by using water and dimethyl sulfoxide to prepare different concentration gradients.

Preparation of a culture medium: 9g of agar-free culture medium powder is accurately weighed, dissolved in 500mL of distilled water, adjusted to pH 7.2-7.4 by 10% NaOH, sterilized at high temperature and high pressure, and cooled for later use (2% of agar needs to be added into a solid culture medium).

Activating strains: taking 1mL of culture medium in a sterile centrifuge tube, unfreezing the strains stored in glycerol, sucking 10 mu L of bacterial liquid into the centrifuge tube, shaking up the bacterial liquid sufficiently, placing the centrifugal tube at 37 ℃ and culturing the bacterial liquid for 10 to 12 hours at 180r/min in a shaking way for later use.

And (3) amplification culture: after the strain is activated, pouring the strain into a conical flask containing 9mL of liquid culture medium, sealing the conical flask, and then placing the conical flask at 37 ℃ for shake culture at 180r/min for 3-4 h.

Determining the number of bacteria: sucking 1mL of the bacterial liquid after the amplification culture and 4mL of the liquid culture medium, uniformly mixing, taking the liquid culture medium as a blank, measuring an OD value at 600nm by adopting a spectrophotometer, and calculating the colony content (CFU/mL) in the bacterial liquid.

Dilution culture: taking the sterilized solid culture medium, airing until the solid culture medium is not hot and is in an unsolidified state, sucking a proper amount of bacterial suspension into the culture medium to enable the bacterial colony content to be 106CFU/mL, fully mixing uniformly, and pouring into a culture dish plate after being tilted.

Punching and dosing: after the culture medium is solidified, a hole (the diameter is about 3 mm) is punched on the culture medium by a puncher, the traditional Chinese medicine solution passes through a 0.22 mu L filter membrane to remove mixed bacteria, a 120 mu L injection hole is sucked, the injection hole is sealed and placed in a refrigerator at 4 ℃ to enable the liquid medicine to diffuse for 4h, the mixture is transferred into an incubator at 37 ℃ to be cultured for 18h, a flat plate is taken out, and the size of a bacteriostatic circle is observed and measured by a vernier caliper.

Data processing: each set of experiments was performed in 3 replicates and averaged, with the results shown in table 1.

TABLE 1 inhibition of various bacteria in vitro at a test concentration of 50mg/L

Group of	Escherichia coli	Staphylococcus aureus (Staphylococcus aureus)	Salmonella	And Pasteurella multocida
					Example 1	A	B	B	A
Example 2	A	B	B	B
					Embodiment 3	B	A	A	B
Example 4	A	A	A	A
					Example 5	B	B	A	C
Comparative example 1	A	A	A	A

Remarking: a: >90%, B: 75-90%, C: <75%

From the results in table 1, the veterinary drug compound preparation can effectively inhibit common pathogenic bacteria of escherichia coli, staphylococcus aureus, salmonella and pasteurella in the chicken breeding process, and has a certain application prospect. Provides a preparation scheme of a veterinary drug 'substitution antibody' product.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same. But one of ordinary skill in the art would understand that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and these modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A veterinary drug 'Tekang' compound preparation is characterized by comprising the following substances by weight: 10-20 parts of lonicera confusa extract, 20-40 parts of macleaya cordata extract, 10-20 parts of scutellaria baicalensis extract, 10-20 parts of astragalus membranaceus extract, 5-20 parts of desmodium extract and 5-20 parts of cassia twig extract.

2. The veterinary drug 'tikan' compound preparation according to claim 1, which is prepared from the following raw materials in parts by weight: 15 parts of lonicera confusa extract, 35 parts of macleaya cordata extract, 15 parts of scutellaria baicalensis extract, 15 parts of astragalus membranaceus extract, 10 parts of desmodium extract and 10 parts of cassia twig extract.

3. A method for preparing the traditional Chinese medicine composition of any one of claims 1 to 2, which is characterized by comprising the following steps: extracting the effective components of the Chinese medicinal materials with water and/or ethanol as extraction solvent by one or more of water extraction, acid extraction and alkali precipitation, ultrasonic-assisted extraction, and water extraction and alcohol precipitation, concentrating, and drying to obtain the Chinese medicinal extract.

4. The preparation method of the traditional Chinese medicine composition according to claim 3, wherein the water extraction method comprises the steps of adding 5-10L of water into 1kg of medicinal materials, soaking for 1-2 h, heating and refluxing for extraction for 1-3 times, each time for 1-3 h, combining filtrates, concentrating and drying to obtain the extract.

5. The method for preparing a Chinese medicinal composition according to claim 3, wherein the acid-base precipitation is carried out by adding 1kg of the medicinal material into 5-10L of dilute hydrochloric acid with pH value of 1-3, soaking for 1-2 h, heating, refluxing, extracting for 1-3 times, each time for 1-3 h, concentrating the extract to 10-30% of the initial volume, adding NaOH solution with pH value of 12-14, adjusting pH value to 10-12, precipitating, vacuum filtering, collecting solid, and drying.

6. The preparation method of the traditional Chinese medicine composition according to claim 3, wherein the ultrasonic-assisted extraction method comprises adding 3-8L ethanol into 1kg of medicinal materials, and ultrasonically extracting for 1-3 times at a power of 25-55 kHz and a temperature of 20-60 ℃ for 0.5-1.0 h each time; concentrating the obtained extracting solution, standing for 10-24 h, centrifuging under the condition of 5000-10000 rpm, taking supernatant, adjusting, concentrating and drying to obtain the extract.

7. The preparation method of the traditional Chinese medicine composition according to claim 3, wherein the water extraction and alcohol extraction method comprises the steps of adding 5-10L of water into 1kg of medicinal materials, soaking for 1-2 h, then heating and refluxing for extraction for 1-3 times, wherein each time lasts for 1-3 h, concentrating an extracting solution to 10% -30% of the initial volume, adding absolute ethyl alcohol until the alcohol content is 80% -90%, standing for 10-24 h at 2-8 ℃, precipitating, carrying out suction filtration to collect solids, and drying the extract.

8. Use of the Chinese medicinal composition of any one of claims 1-2 in the preparation of a veterinary drug "tikan" compound preparation.

9. The method as claimed in claim 8, wherein the said application is the control of pathogenic bacteria commonly found in chicken breeding processes: escherichia coli, staphylococcus aureus, salmonella, and pasteurella.

10. According to claim 8, the application object includes, but is not limited to, chicken breeding industry, and can be any breeding industry or a class of breeding industries.