CN115843694B - A device for rapid rooting and domestication of soybean tissue culture seedlings - Google Patents
A device for rapid rooting and domestication of soybean tissue culture seedlings Download PDFInfo
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- CN115843694B CN115843694B CN202310015385.8A CN202310015385A CN115843694B CN 115843694 B CN115843694 B CN 115843694B CN 202310015385 A CN202310015385 A CN 202310015385A CN 115843694 B CN115843694 B CN 115843694B
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Abstract
本发明属于组培苗生根培养装置技术领域，具体涉及一种用于大豆组培苗快速生根驯化的装置，包括盒体，盒体由两部分半盒可拆卸拼接而成，半盒的顶壁设有允许组培苗枝叶穿出的半穿孔，半盒的内壁设有卡接盘，卡接盘上开设有卡接口；当两个半盒拼接成盒体后，两个半盒上的半穿孔可拼接成一个完整的穿接孔，卡接盘可拼接成一个盘状结构，卡接口也拼接成完整的卡口结构；其中一个半盒的顶壁和底壁之间通过循环管连通，循环管上设有循环泵，循环管的管身处还连通设有贮液箱。本发明设置适用组培苗的培育，打开盒体，直接观察、测量根系。
The invention belongs to the technical field of tissue culture seedling rooting and cultivating devices, and specifically relates to a device for rapid rooting and domestication of soybean tissue culture seedlings. There is a half-perforation that allows the branches and leaves of the tissue culture seedlings to pass through. The inner wall of the half box is provided with a clamping plate, and a clamping interface is provided on the clamping plate; when the two half-boxes are spliced into a box body, the half-perforations on the two half-boxes can be Spliced into a complete through hole, the clamping disk can be spliced into a disk-shaped structure, and the clamping interface is also spliced into a complete bayonet structure; the top wall and bottom wall of one of the half boxes are connected through a circulation tube, and the circulation tube A circulation pump is provided, and the body of the circulation pipe is also connected with a liquid storage tank. The invention is suitable for cultivating tissue culture seedlings. The box can be opened to directly observe and measure the root system.
Description
Technical Field
The invention belongs to the technical field of tissue culture seedling rooting culture devices, and particularly relates to a device for rapid rooting and domestication of soybean tissue culture seedlings.
Background
The tissue culture technology is a common technology for screening high-quality plants, various tissue culture methods can be developed by cultivating tissue culture seedlings, the influence of different plant growth promoters and transgenic preparations on the plant seedlings is explored, soybeans are common tissue culture seedling cultivation objects, the root system condition of the tissue culture seedlings is researched, and the method has great help to the development work of rhizobia. Since the tissue culture seedlings are the result of the totipotent development of plant cells of the explants, and the development of soybean of the explants is performed in an agar-containing medium, many of the tissue culture seedlings are grown in the agar medium at the initial stage. The agar blocks are solid, the root systems of the tissue culture seedlings grow in a dispersed manner in the agar blocks, stripping is not easy, and the subsequent physiological state records such as root length, root number and the like are affected.
The current device for cultivating plant root system solves the above-mentioned technical problem of how to observe root system conveniently, such as a water planting device suitable for soybean full growth period test disclosed in chinese patent CN106035036a, comprising: the transparent water planting case, the water planting incasement wall is close to the top and is provided with a plurality of field planting bottom plates, and the inside vertical a plurality of baffles that are provided with of water planting case, a plurality of independent separating tank is separated into with the water planting case to a plurality of baffles, and every baffle is close to the bottom and is equipped with the through-hole, and every separating tank lateral wall is equipped with the flowing back hole. The water planting device is simple in structure, the water planting box is made of transparent materials, so that the root system development condition of plants can be observed or measured, and the arrangement of the plurality of field planting bottom plates, the plurality of partition plates, the through holes and the liquid draining holes of the plurality of partition plates is convenient for observation and research, so that the root system development condition of plants can be observed or measured at any time. However, the initial stages of the tissue culture seedlings are all grown in agar medium, the required medium is solid, the above patent provides influence by liquid nutrient medium, and the method cannot be suitable for cultivating the tissue culture seedlings.
In view of the above, it is desirable to develop a device that is suitable for rooting cultivation of tissue culture seedlings and is convenient for observing root growth.
Disclosure of Invention
In order to solve the technical problems, the invention provides a device for rapid rooting and domestication of soybean tissue culture seedlings.
The invention aims to provide a device for rapid rooting and domestication of soybean tissue culture seedlings, which comprises a box body, wherein the box body is formed by detachably splicing two half boxes, the top wall of each half box is provided with a half perforation allowing the branches and leaves of the tissue culture seedlings to pass through, the inner wall of each half box is provided with a clamping disc, and the clamping discs are provided with clamping interfaces;
when the two half boxes are spliced into the box body, the half through holes on the two half boxes can be spliced into a complete through hole, the clamping discs corresponding to the two half boxes can be spliced into a disc-shaped structure, and the clamping interfaces corresponding to the two half boxes are spliced into a complete bayonet structure;
the top wall and the bottom wall of one half box are communicated through a circulating pipe, a circulating pump is arranged on the circulating pipe, and a liquid storage tank is further communicated at the pipe body of the circulating pipe.
Preferably, the device for rapid rooting and domestication of soybean tissue culture seedlings is characterized in that the box body is formed by rotationally connecting two half boxes, and after the two half boxes are spliced into the box body, the edge contact position of the half boxes is sealed and is not leaked.
Preferably, the device for rapid rooting and domestication of soybean tissue culture seedlings is characterized in that a guide tube is arranged at the bottom of the clamping interface.
Preferably, in the device for rapid rooting and domestication of soybean tissue culture seedlings, the guide pipes are semicircular tables, and after the two semicircular boxes are spliced into the box body, the two guide pipes can be spliced into a circular truncated cone-shaped structure with the diameter of an upper end pipe orifice being larger than that of a lower end pipe orifice.
Preferably, the device for rapid rooting and domestication of soybean tissue culture seedlings is characterized in that the guide pipe is detachably connected to the clamping connector in a clamping mode, an inoculation opening is formed in the top of the guide pipe, water-permeable paper is adhered to the bottom wall of the guide pipe, viscosity of the water-permeable paper disappears after the water-permeable paper is soaked in liquid, and the water-permeable paper falls.
Preferably, in the device for rapid rooting and domestication of soybean tissue culture seedlings, a sealing band is arranged at the edge of the side wall of the guide tube, and when two guide tubes are spliced, the sealing band seals the joint.
Preferably, the device for rapid rooting and domestication of soybean tissue culture seedlings is characterized in that an elastic strip is arranged at the edge of the clamping interface, which is used for being connected with the guide pipe, and the guide pipe is tightly abutted with the elastic strip when being clamped into the clamping interface.
Preferably, in the device for rapid rooting and domestication of soybean tissue culture seedlings, a plurality of clamping interfaces can be arranged on one clamping disc, and the number of the clamping interfaces on two clamping discs is equal.
Preferably, the device for rapid rooting and domestication of soybean tissue culture seedlings is characterized in that the liquid storage box is provided with a liquid inlet and a liquid outlet.
Preferably, the device for rapid rooting and domestication of soybean tissue culture seedlings can be prepared from transparent materials except a circulating pump, a circulating pipe and a liquid storage tank.
Compared with the prior art, the invention has the following beneficial effects:
the precondition of the generation of the tissue culture seedling is that the explant is induced to be cultured into the callus, and then the callus is proliferated and cultured into the tissue culture seedling, the culture medium is required to be replaced in the process, and the tissue culture seedling is also required to be transplanted, so that the tissue damage is easy to cause, and the culture effect of the tissue culture seedling is affected. Therefore, the invention sets a storage space to store the solid induction culture medium, so that the explant can develop into the callus, and then the liquid proliferation culture medium is injected into the box body, so that the callus can continue to differentiate, root and germinate, and the tissue culture seedling can be formed. In the process of replacing the culture medium, the original solid culture medium is not required to be stripped manually, but is gradually stripped through the water flow impact effect, so that the culture medium is automatic and roots are not damaged. After the box body is opened, the root system is directly observed and measured, the plant body is clamped at the bayonet structure (or the guide tube) again, the box body is combined, and after sterilization, the liquid culture operation can be continued.
In the embodiment 2 of the invention, the dropping permeable paper is arranged, the viscosity of the glue for adhering the permeable paper disappears under the long-time soaking action of the liquid rooting culture medium, the permeable paper drops, the root system is gathered in the root system growth space between the clamping disc and the bottom wall of the half box, and the solid culture medium is gradually separated from the plant body due to continuous flushing of water flow, so that the operations of moving the solid culture medium and peeling the solid culture medium are omitted.
Drawings
Fig. 1 is a schematic view showing the development of a device for rapid rooting and domestication of soybean tissue culture seedlings in embodiment 1;
fig. 2 is a perspective view of the combined box body of embodiment 1 of the present invention;
FIG. 3 is a view showing the structure of the guide tube according to embodiment 2 of the present invention after being unfolded (for convenience of marking, the water permeable paper is cut into two halves in the figure);
FIG. 4 is a diagram showing the connection structure of the guide tube and the half box (for convenience of marking, the water permeable paper is cut into two halves);
Detailed Description
In order that those skilled in the art will better understand the technical scheme of the present invention, the present invention will be further described with reference to specific embodiments and drawings.
In the description of the present invention, unless otherwise specified, all reagents are commercially available and methods are conventional in the art.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention. The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second" may include one or more such features, either explicitly or implicitly; in the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
Example 1
The embodiment 1 of the invention provides a device for rapid rooting and domestication of soybean tissue culture seedlings, which is shown in fig. 1-2, and comprises a box body 1, wherein the box body 1 is formed by rotationally connecting two half boxes 11, and the two half boxes 11 are symmetrically arranged, attractive and easy to combine and open. In the embodiment of the present invention, the case 1 is provided to be formed by splicing two half-cases 11 in a semi-cylindrical shape, or by splicing two half-cases 11 in a cubic shape. The outer wall of the box body 1 is solid, and the inside is hollow, that is, after the two half boxes 11 are spliced, the opposite surfaces of the two half boxes 11 are open, and after the two half boxes 11 are spliced into the box body 1, the edges of the half boxes 11 are sealed and leak-free. Specifically, referring to fig. 1, one of the two half boxes 11 is hinged by a hinge or a rotation shaft, and the other is connected by a latch, and a sealing gasket is arranged at the edge of the half box 11, so that when the two half boxes 11 are buckled together, the sealing gasket is in close contact to realize the effect of no leakage. The culture solution or the solid culture medium for culturing the soybean tissue culture seedlings for holding the liquid in the box body 1 has the advantages that the method for combining the liquid and the solid culture is simple to operate, the transparency of the liquid is good, and the observation of a scientific research worker on a root system is not blocked. Because the root system likes dark environment, so the surface cover of box body 1 is with the shading cover of light-proof material preparation, and shading cover adopts detachable elasticity cover preparation, easy to assemble and dismantlement.
The roof of half box 11 is equipped with half perforation 12 that allows the tissue culture seedling branch and leaf to wear out, and after two half boxes 11 splice into box body 1, half perforation 12 on two half boxes 11 can splice into a complete cross-under hole, and the cross-under hole is circular, square or polygon all can.
The inner wall of the half box 11 is provided with a clamping disc 13, the clamping disc 13 is provided with a clamping interface 14, after the two half boxes 11 are spliced into the box body 1, the clamping discs 13 corresponding to the two half boxes 11 can be spliced into a complete disc-shaped structure, the clamping interfaces 14 corresponding to the two half boxes 11 can also be spliced into a complete bayonet structure, and the bayonet is round, square or polygonal. The bayonet structure is used for clamping the tissue culture seedlings, preventing the tissue culture seedlings from moving in disorder in the box body 1 or preventing the tissue culture Miao Diaola. In one half-box 11, the space between its catch tray 13 and the top wall of the half-box 11 is a storage space 15, the storage space 15 being used for placing a solid agar-containing culture medium block.
The top wall and the bottom wall of one half box 11 are communicated through a circulating pipe 2, a circulating pump 3 is arranged on the circulating pipe 2, a liquid storage tank 4 is further communicated at the pipe body of the circulating pipe 2, the liquid storage tank 4 is connected between the top wall and the bottom wall of the half box 11, the liquid storage tank 4 is provided with a liquid inlet and a liquid outlet, the liquid inlet is used for injecting new culture liquid into the liquid storage tank 4, the liquid outlet is used for discharging old culture waste liquid, switch valves are arranged at the liquid inlet and the liquid outlet, and liquid inlet and liquid outlet operations are controlled through the switch valves.
The working principle of this embodiment is as follows: firstly, the two half boxes 11 are opened as shown in fig. 1, a solid culture medium is manufactured in a storage space 15, the solid culture medium adopts a formula of an induction/proliferation culture medium, the explant is convenient to grow into callus, even a small part of adventitious buds and embryoids are differentiated, the storage space 15 acts as a culture dish, the mass percentage of agar is controlled to be 5-6%, thus, the subsequent liquid circulation can enable the culture medium to be easily broken, and the thickness of the solid culture medium is preferably set to be less than 1/2 of the thickness of the storage space 15, so that the solid culture medium is convenient to inoculate and move. Then, the two half-boxes 11 are combined, and a sterile forceps or other instrument is inserted into the storage space 15 from the through hole for inoculation. After inoculation, the device is placed in a climatic box or an incubator, soybean explants are cultured into callus, even a small part of adventitious buds and embryoids are differentiated, at the moment, the volume of plants is increased, the tolerance is enhanced, and rooting culture operation can be performed. At this time, the solid culture medium and the plant body thereon are stirred to be positioned right above the bayonet structure by using the sterile appliance to extend into the storage space 15 from the through hole, and the solid culture medium cannot fall from the bayonet structure, namely, the size of the bayonet structure is set to be smaller than that of the solid culture medium; the liquid storage tank 4 is placed with a sterile liquid rooting culture medium, the circulating pump 3 is started to enable the liquid rooting culture medium to circulate clockwise as shown in fig. 1, so that the flow of liquid has a downward induction effect on the growth direction of root systems, the newly grown root systems are induced to continuously grow downwards in the direction below the bayonet structures, the newly grown root systems are gathered in the root system growth space 16 of the clamping disc 13 and the bottom wall of the half box 11, the solid culture medium is gradually separated from plant bodies due to continuous flushing of water flow, the operation of stripping the solid culture medium is omitted, after the rooting culture medium in the box body 1 is discharged, the box body 1 is opened, the root systems can be directly exposed, and the root systems can be directly observed, measured or taken out. Experiments can be performed in advance to record the flow rate of rooting medium that allows rapid stripping of solid medium.
In addition, as a plurality of leguminous plant roots can be symbiotic with rhizobia, when the root systems of the tissue culture seedlings grow properly, rhizobia bacterial liquid can be replaced in the liquid storage tank 4, bacterial liquid is sent into the root system growth space 16 through the action of the circulating pump 3, and the root system growth space 16 is prepared by adopting transparent materials, so that the symbiotic condition of soybean root systems and rhizobia and the root system growth condition can be conveniently observed, and multiple purposes are achieved.
Preferably, the bottom of the clamping port 14 is provided with the guide pipe 17, in this embodiment, the guide pipe 17 is in a semicircular table shape, after the two half boxes 11 are spliced into the box body 1, the two guide pipes 17 can be spliced into a circular table structure with the diameter of the pipe orifice at the upper end larger than that of the pipe orifice at the lower end, rooting culture solution flows downwards along the guide pipe and is converged at the bottom of the circular table structure, root systems also grow towards the bottom of the circular table structure, and finally the pipe orifice at the lower end of the circular table structure grows out and enters the growth space 16. And, the plant body is caught between the two guide pipes 17 so as not to fall. After the box body 1 is opened, the root system is directly observed and measured, the plant body is clamped at the guide pipe 17 again, the box body 1 is combined, and after sterilization by the disinfectant, the liquid culture operation can be continued.
It should be noted that, 1-4 clamping interfaces 14 can be arranged on one clamping disc 13, so that 1-4 tissue culture seedlings can be correspondingly cultivated. Note that the whole culture apparatus is sterilized in advance to prevent contamination during the culture of the tissue culture seedlings. The sterile cotton can be plugged at the cross-connection hole formed by the half-perforation 12, and the thickness of the edge of the cross-connection hole can be set to be 1-3cm, so that the sterile cotton can be plugged conveniently.
Example 2
The device for rapid rooting and domestication of soybean tissue culture seedlings provided by the embodiment is similar to the structure of embodiment 1, and is different in that, referring to fig. 3-4, the guide tube 17 is detachably clamped on the clamping interface 14, an elastic strip is arranged at the edge of the clamping interface 14, which is used for being connected with the guide tube 17, the elastic strip is a rubber strip or a latex strip, and when the guide tube 17 is clamped into the clamping interface 14, the guide tube 17 is tightly abutted with the elastic strip to generate friction force, so that the clamping interface 14 and the guide tube 17 are clamped.
The two guide pipes 17 are spliced into a cylindrical shape or a truncated cone structure similar to that of embodiment 1, the inoculation openings 171 are formed in the tops of the guide pipes 17, sealing strips are arranged on the edges of the side walls of the guide pipes 17, when the two guide pipes 17 are spliced, the connecting parts are sealed by the sealing strips, and when the two guide pipes 17 are spliced, the corresponding inoculation openings 171 are spliced into a finished inoculated opening. The side wall of the guide tube 17 is solid, the bottom wall of the guide tube 17 is adhered with permeable paper 172 through glue, the viscosity of the glue disappears after long-time liquid soaking for 6-30 hours, and the permeable paper 172 falls off. Note that the material of the water permeable paper 172 is selected, for example, a nonwoven fabric or a medical film with small voids, which can block the non-solidified liquid medium containing agar.
The working principle of this embodiment is as follows, the manufactured solid medium is placed in the guide tube 17, the viscosity of the glue is still at this moment, the permeable paper 172 can block and bear the weight of the solid medium, the solid medium adopts the formulation of the induction/proliferation medium, the storage space 15 is used for storing the guide tube 17, the top tube orifice of the guide tube 17 can extend out of the through hole, and the sterile cotton plug orifice is used, the sterile instrument extends into the top tube orifice of the guide tube 17, the explant is inoculated, after inoculation, the device is placed in a climatic box or incubator, the explant is cultured into callus, even a small part of adventitious buds and embryoids are differentiated, at this moment, the volume of the plant becomes large, the tolerance is enhanced, and rooting culture operation can be performed. The liquid storage tank 4 is placed with a sterile liquid rooting culture medium, the circulating pump 3 is started to enable the liquid rooting culture medium to circulate clockwise as shown in fig. 1, so that the flow of liquid has a downward induction effect on the growth direction of root systems, the newly grown root systems are induced to continuously grow downwards in the direction below the bayonet structure, the viscosity of glue for adhering the permeable paper 172 disappears under the long-time soaking effect of the liquid rooting culture medium, the permeable paper 172 falls off and gathers in the root system growth space 16 of the clamping disc 13 and the bottom wall of the half box 11, and due to continuous flushing of water flow, the solid culture medium is gradually separated from plant bodies, the operation of moving the solid culture medium and peeling the solid culture medium is omitted, after the rooting culture medium in the box body 1 is discharged, the box body 1 is opened, the growth condition of the root systems is observed, or the root is taken.
In order to facilitate observation of the rough form of the root system, other parts except the circulating pump 3, the circulating pipe 2 and the liquid storage tank 4 can be made of transparent materials.
It should be noted that, the connection relationships of the components not specifically mentioned in the present invention are all default to the prior art, and the connection relationships of the structures are not described in detail because they do not relate to the invention points and are common applications of the prior art.
It should be noted that, when numerical ranges are referred to in the present invention, it should be understood that two endpoints of each numerical range and any numerical value between the two endpoints are optional, and because the adopted step method is the same as the embodiment, in order to prevent redundancy, the present invention describes a preferred embodiment. While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (5)
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310015385.8A CN115843694B (en) | 2023-01-05 | 2023-01-05 | A device for rapid rooting and domestication of soybean tissue culture seedlings |
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| CN202310015385.8A CN115843694B (en) | 2023-01-05 | 2023-01-05 | A device for rapid rooting and domestication of soybean tissue culture seedlings |
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| CN115843694B true CN115843694B (en) | 2024-03-19 |
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| CN115843694A (en) | 2023-03-28 |
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