CN208446153U - A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus - Google Patents
A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus Download PDFInfo
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- CN208446153U CN208446153U CN201820858724.3U CN201820858724U CN208446153U CN 208446153 U CN208446153 U CN 208446153U CN 201820858724 U CN201820858724 U CN 201820858724U CN 208446153 U CN208446153 U CN 208446153U
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Abstract
The utility model discloses a kind of bottomless alms bowls for eggplant verticillium wilt to hurt root classification inoculation apparatus, it include: multiple rows of nutritive cube and nutritive cube bracket, multiple rows of nutritive cube is arranged side by side in nutritive cube bracket, each row's nutritive cube includes bottom plate, the first side plate and the second side plate, offers multiple apertures on bottom plate；First side plate and the second side plate are respectively foldably set to the left and right sides of bottom plate, multiple first connection wings and multiple second connection wings are respectively set in the opposite side of first side plate and the second side plate, the first connection wing and the second connection wing one-to-one correspondence plug together connection, and the first side plate, the second side plate, the first connection wing and the second connection wing surround multiple rows of nutritive cube being separated into multiple individual nutritive cubes.The utility model hurts root inocalation method using bottomless alms bowl and carries out Resistance Identification, and in seedling stage in Disease Resistance Identification, bottomless alms bowl hurts root inocalation method and better reflects the true resistance of eggplant resisting verticillium, and falls ill and stablize, uniformly, and onset speed is very fast, high-efficient.
Description
Technical field
The utility model relates to plant resistance to environment stress identification technology field, in particular to a kind of bottomless alms bowl for eggplant verticillium wilt
Hurt root classification inoculation apparatus.
Background technique
Eggplant verticillium wilt is a kind of global soil-borne vascular bundle disease that verticillium dahliae infects harm.The pathogen is posted
Main range is very extensive, in addition to infecting eggplant, can also infect and endanger more than 600 kinds of cotton, tomato, sunflower, potato, strawberry etc.
Crop.The U.S. in 1914 finds cotton verticillium wilt caused by verticillium dahliae for the first time, and nineteen thirty-five, China was because introducing american cotton product
It plants and incoming verticillium wilt germ.Eggplant verticillium wilt only locally occurs in the Northeast of China at the beginning of the 50's of 20th century, with solanberry
The expansion of class vegetable cultivation area and spread rapidly, morbidity range increasingly south expand, China's eggplant Main Cultivation area is sent out at present
The raw disease.Eggplant verticillium wilt disease incidence is high, and general time disease incidence is 40%~50%, the serious time up to 70% or more, very
It is received to kind of a mistake is ruined, serious influence is all caused on eggplant quality and yield, it has also become restrict the important disease of eggplant industry development.
Verticillium wilt is typical soil-borne vascular bundle disease, and Microsclerotia is verticillium dahliae major survival structure and First aggression
Source, since its resistance is strong, the time-to-live is long in the soil, and chemical prevention is undesirable；Although crop rotation effectively, gives vegetables
Production crops for rotation arrangement brings practical difficulty；Graft technology can effectively mitigate eggplant verticillium wilt, but bring grafting technology and
Cost problem is difficult application in production practice.Breeding and using disease-resistant variety be prevention and treatment eggplant verticillium wilt most directly, peace
Entirely, efficiently one of measure, and screen the important foundation work that Resistant gerplasm resource is breeding for disease resistance.Infected Field method is resistance
Main foundation most traditional in identification, using most common method, when expert's conclusion is still new varieties authorization so far.But it reflects
Fixed cycle is long, by seasonal effect, expert evidence limited amount.In addition, pathogen quantity does not know, is unevenly distributed and gas in sick nursery
Waiting the series of factors such as changes in environmental conditions influences the accuracy of qualification result.
Artificial inoculation on seedling method, not by seasonal effect, the better control selections pressure of energy can a large amount of, repetition screening material.Mesh
Before, root dipping inocalation method is generallyd use, its advantage is that morbidity is uniform, rapid onset.But when using root dipping inoculation, each kind intercurrent disease is equal
It is heavier, the Resistant Difference between kind to verticillium wilt cannot be accurately and efficiently showed, it is poor with Infected Field Adult plant Incidence level
It is different larger, the true resistance of kind cannot be reflected.
Therefore, it establishes a set of quick, stable, accurate and effective, embodies the seedling of the true disease resistance of Eggplant Varieties to greatest extent
Phase artificial method of resistance identification and identification apparatus are a technical problems urgently to be resolved.
Utility model content
The utility model provides a kind of bottomless alms bowl for eggplant verticillium wilt and hurts root classification inoculation apparatus, and which solve above-mentioned existing
Seedling Inoculation technical disadvantages.
Technical problem to be solved in the utility model is achieved through the following technical solutions:
A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus, comprising: multiple rows of nutritive cube and nutritive cube bracket, institute
It states multiple rows of nutritive cube to be set in nutritive cube bracket, each row's nutritive cube includes bottom plate, the first side plate and the second side plate, described
Multiple apertures are offered on bottom plate；First side plate and second side plate are respectively foldably set to a left side for the bottom plate
Multiple first connection wings and multiple second connections are respectively set in the opposite side of right two sides, first side plate and second side plate
The wing, the first connection wing and the second connection wing one-to-one correspondence plug together connection, first side plate, second side plate,
The first connection wing and the second connection wing surround multiple rows of nutritive cube being separated into multiple individual nutritive cubes.
Preferably, in above-mentioned technical proposal, it further includes using that the bottomless alms bowl for eggplant verticillium wilt, which hurts root classification inoculation apparatus,
In the apparatus for placing for holding bacteria suspension, multiple rows of nutritive cube is set in the apparatus for placing by the nutritive cube bracket.
Preferably, in above-mentioned technical proposal, the front end of the first connection wing is inwardly formed recess, and the multiple second connects
The front end for connecing the wing is outwardly formed protrusion, and the recess and described project through are clamped connection.
Preferably, in above-mentioned technical proposal, multiple grooves are additionally provided on first side plate and second side plate.
Preferably, in above-mentioned technical proposal, first side plate, second side plate, the bottom plate, first connection
The plastics that the wing, the second connection wing are integrally formed.
Preferably, in above-mentioned technical proposal, the nutritive cube bracket includes bottom plate and four blocks of coaming plates, the coaming plate with it is described
The rotatable connection of bottom plate, the connection fixed by snap of adjacent coaming plate.
Preferably, in above-mentioned technical proposal, multiple rows of nutritive cube is eight rows, the first side plate of each row's nutritive cube, second side
Plate, the first connection wing and the second connection wing surround the nutritive cube of each row being separated into four individual kind of plant holes.
The utility model above-mentioned technical proposal, has the following beneficial effects:
Bottomless alms bowl according to the present utility model for eggplant verticillium wilt hurts root classification inoculation apparatus, and seedling stage, bottomless alms bowl hurt piece-root grafting
The moderate and uniformity of kind method morbidity, more meets the true resistance of eggplant verticillium wilt.Its whole process more really simulates cause of disease
Bacterium is infected from root wound, guarantees that every plant of eggplant seedling uniformly carries disease germs, and practical uniformity of falling ill same processing seldom occurs, has
Plant morbidity it is serious, some morbidities are light not to fall ill even, or the inconsistent situation of intercurrent disease of repetition.In conclusion seedling
In phase Disease Resistance Identification, bottomless alms bowl hurts root inocalation method and better reflects the true resistance of eggplant resisting verticillium, and falls ill and stablize,
Even, onset speed is very fast, high-efficient.
Detailed description of the invention
Fig. 1 is that the bottomless alms bowl for eggplant verticillium wilt of the utility model hurts multiple rows of nutritive cube of root classification inoculation apparatus (wherein
One row) top view.
Fig. 2 is that the bottomless alms bowl for eggplant verticillium wilt of the utility model hurts multiple rows of nutritive cube of root classification inoculation apparatus (wherein
One row) bottom view.
Fig. 3 is that the bottomless alms bowl for eggplant verticillium wilt of the utility model hurts the nutritive cube bracket signal of root classification inoculation apparatus
Figure.
Fig. 4 be the bottomless alms bowl for eggplant verticillium wilt of the invention hurt root classification inoculation apparatus nutritive cube bracket and multiple rows of nutrition
Alms bowl assembling figure.
Fig. 5 is that the bottomless alms bowl for eggplant verticillium wilt of the utility model hurts the apparatus for placing schematic diagram of root classification inoculation apparatus.
Specific embodiment
Specific embodiment of the utility model is described in detail below, in order to further understand the utility model.
All experimental methods used are conventional method unless otherwise specified in following embodiment.In following embodiment
Material, reagent used etc. can be obtained through commercial channels unless otherwise specified.
Embodiment 1
A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus, comprising: multiple rows of nutritive cube 1 and nutritive cube bracket 2.
Specifically:
Multiple rows of nutritive cube 1 is arranged side by side in nutritive cube bracket 2, and each row's nutritive cube 1 includes bottom plate 15, the first side
Plate 11 and the second side plate 12 offer multiple apertures 16 on bottom plate 15；First side plate 11 and the second side plate 12 are respectively foldably
It is set to the left and right sides of bottom plate 15, multiple first connections wings 13 are respectively set in the opposite side of the first side plate 11 and the second side plate 12
With multiple second connection wings 14, the first connection wing 13 and the second connection one-to-one correspondence of the wing 14 plug together connection, the first side plate 11, second
Side plate 12, first connects the wing 13 and the second connection wing 14 surrounds multiple rows of nutritive cube 1 being separated into multiple individual nutritive cubes.
Preferably, the front end of the first connection wing 13 is inwardly formed recess, and the front end of the second connection wing 14 is outwardly formed protrusion,
It is recessed and projects through clamping and connects.First side plate 11, the second side plate 12, bottom plate 15, first connect the wing 13 and the second connection wing
14 are integrally formed, and are additionally provided with multiple grooves 17 on the first side plate 11 and the second side plate 12.The water when setting of groove facilitates watering
Dividing can be smoothly to dirty.It is highly preferred that multiple rows of nutritive cube 1 is eight rows, the first side plate 11, the second side plate of each row's nutritive cube
12, the first connection wing 13 and the second connection wing 14 surround the nutritive cube 1 of each row being separated into four individual kind of plant holes.Kind
Eggplant seedling is planted in plant hole.Certainly, the utility model is not limited thereto, and those skilled in the art can select and design as needed
The quantity of the kind plant hole of suitable number of rows and each row.
Preferably, nutritive cube bracket 2 includes: backing board 21 and muti-piece coaming plate 22, and coaming plate 22 and backing board 21 are rotatable
Connection, the connection fixed by snap of adjacent coaming plate.
The conventional container for holding bacteria suspension of the present embodiment cooperation uses.
Embodiment 2
A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus, and the device is substantially same as Example 1, difference
Be in, in addition to including multiple rows of nutritive cube 1 and nutritive cube bracket 2, the device further include: for holding the apparatus for placing of bacteria suspension
3。
It is a kind of to hurt method that root classification inoculation apparatus is inoculated with and identified (with eggplant verticillium wilt seedling resistance mirror using bottomless alms bowl
It is set to example):
(1) nursery: choosing full seed, impregnates 15min under 55 DEG C of water temperatures, stirs when impregnating, and then clear water soaks
After steeping 48h, seed is pulled out from clear water, is drained away the water, intermittent warming vernalization is carried out after binding up with gauze, is showed money or valuables one carries unintentionally to seed
Afterwards, it uniformly broadcasts in the nutritive cube that lower section has aperture；
(2) prepared by spore suspension: eggplant verticillium wilt bacterial strain being activated on PDA medium plate, in 25 DEG C -26
DEG C culture 4-7d, picking 2-3mm fungus block 2-3 block is transferred in 150mL PD fluid nutrient medium, in 25 DEG C -26 DEG C, 130rpm/min
After shaking table culture 4-7d, four layers of sterile gauze filtering are diluted by aqua sterilisa, are configured to certain density spore suspension and (connect
Kind concentration is 1 × 107A spore/mL)；
(3) it is inoculated with:, the eggplant that with the scissors that sterilized is grown nutritive cube below tapping long to 3-4 piece true leaf to eggplant seedling
After seedling fibrous root is cut, nutritive cube is immersed in the apparatus for placing equipped with spore suspension and impregnates inoculation, soaking time 10-
30min, soaking depth are 1-2 centimetres；
(4) it observes: after inoculation, after verticillium wilt typical case disease symptom to appear, progress first time incidence survey, later every 10
Its investigation is primary, until morbidity is stablized, no longer extends；Disease index investigation is carried out by grade scale, and calculates disease index.
The comparison of 1 inoculation method of comparative example
Root dipping method, root-pouring method is respectively adopted, mixes local method and injection method is inoculated with.Eggplant Varieties are dragon miscellaneous eggplant No. 10
(commercially available), one heart stage of five leaves, inoculum density are 1 × 107A spore/mL.
Root dipping method: seedling is gently pulled up, and cleans root dipping 10min after the soil of root.
Root-pouring method: the pouring root 10ml spore suspension after oblique partial application at rhizosphere 1cm.
It mixes local method: spore suspension is uniformly admixed in soil, soil inoculation final concentration of 1 × 107A spore/mL.
Injection method: 10ml spore suspension is injected in eggplant seedling basal part of stem with sterilizing syringe needle.
The result shows that 5 kinds of inoculation methods, most fast and serious morbidity is root dipping inocalation method, shows bottom within inoculation the 8th day
Portion's blade turn yellow or it is half of move back green symptom, the 25th day average disease refers to up to 85.6, significant with other processing differences, but falls ill excessively
Seriously, it is unfavorable for distinguishing anti-, emotion condition；Pouring root inocalation method is in inoculation test using relatively more, the disease of this inoculation the 25th day
Feelings index is 77.0, but its morbidity is uneven, and while being inoculated with hurts the more difficult control of root, it is sometimes desirable to slow seedling, to test operation and
The accuracy of data brings difficulty；Injection inoculation method inoculation efficiency is low, operating difficulties, falls ill undesirable, and mixes native inocalation method hair
Longer the time required to sick, the 12nd talent of inoculation starts aobvious disease.Bottomless alms bowl inocalation method morbidity is very fast, uniform, and seeded process operates
Simplicity, the average disease index of inoculation the 25th day are 80.6, and disease index is consistent with the identification anti-emotion condition of host.
The comparison of 1 Verticillium Wilt Pathogen of Eggplant different vaccination method of table
The utility model has the advantage that
Seedling stage bottomless alms bowl hurts the moderate and uniformity of root inocalation method morbidity, more meets the true resistance of eggplant verticillium wilt.Test
In use the commonly used four kinds of inoculation methods of soil-borne disease and hurt root inocalation method with bottomless alms bowl and compare, more comprehensive investigation
The benefit and disadvantage of each method.It is at bowl lower opening, after the fibrous root of exposing is cut, integrally that bottomless alms bowl, which hurts root inocalation method,
It is placed in spore suspension and impregnates, whole process more really simulates pathogen and infects from root wound, guarantees every plant of eggplant
Seedling uniformly carries disease germs, practical uniformity of falling ill, and same processing seldom occurs, and some plant morbidities are serious, and some morbidities are gently even
It does not fall ill, or the inconsistent situation of intercurrent disease of repetition；And for many years with us, certainly to these Eggplant Germplasm Resources crop fields
The data result of resisting verticillium is more consistent in right sick nursery.Therefore, in seedling restance identification, bottomless alms bowl hurts root inocalation method more can body
Reveal the true resistance of eggplant resisting verticillium, and fall ill and stablize, uniformly, onset speed is very fast, high-efficient.
The number of the nutritive cube of the utility model designs multiple groups as needed, and each group is preferably 4, facilitates every in experiment
The duplicate quantity of group.Multiple rows of nutritive cube is directly placed side by side, can take out or move as needed and is therein a row or multi-row.
The device use for holding the apparatus for placing of bacteria suspension, a small amount of inoculation bacteria suspension can be put into the device
Complete inoculation (1-2 centimetres of depth).Plurality of rows of nutritive cube can be used as the immersion apparatus for placing an of overall time, and one
Secondary completion inoculation, easy to operate and inoculation is convenient, and morbidity is uniform；Simultaneously for the lower verticillium wilt bacterial strain of spore production rate, save
A large amount of bacteria suspensions.
Apparatus for placing is used not only for bacteria suspension immersion, in seedling stage the non-seeded stage, can be by multiple rows of nutritive cube and nutritive cube
Bracket is placed in the device, and in daily watering management, can play is prevented from being splashed due to watering, trickles and pathogen is caused to pass
It broadcasts, infect the clear water eggplant seedling that is not inoculated with of control and canopy room for other healthy eggplant seedlings.When needing to be inoculated with, device conduct again
Apparatus for placing uses, and bacteria suspension can reuse after impregnating, if inoculation disk quantity is more, can repeat to use down criticize seedling after
It is continuous to impregnate, bacteria suspension is outwelled after immersion, multiple rows of nutritive cube and nutritive cube bracket also continue being placed in apparatus for placing, convenient for management.
Although the utility model is disclosed as above with embodiment, so it is not intended to limit the utility model, any ability
Field technique personnel can make a variety of different selections and modification, therefore this without departing from the spirit and scope of the utility model
The protection scope of utility model is limited by claims and its equivalents.
Claims (6)
1. a kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus characterized by comprising multiple rows of nutritive cube (1) and
Nutritive cube bracket (2), multiple rows of nutritive cube (1) are arranged side by side in the nutritive cube bracket (2), and each row's nutritive cube wraps
Bottom plate (15), the first side plate (11) and the second side plate (12) are included, offers multiple apertures (16) on the bottom plate (15)；Described
Side plate (11) and second side plate (12) are respectively foldably set to the left and right sides of the bottom plate (15), and described first
The multiple first connections wing (13) and multiple second connection wings are respectively set in the opposite side of side plate (11) and second side plate (12)
(14), first connection wing (13) and second connection wing (14) correspond and plug together connection, first side plate (11),
Second side plate (12), first connection wing (13) and second connection wing (14) are surrounded multiple rows of nutritive cube
(1) multiple individual nutritive cubes are separated into.
2. a kind of bottomless alms bowl for eggplant verticillium wilt according to claim 1 hurts root classification inoculation apparatus, which is characterized in that institute
It states and hurts root classification inoculation apparatus for the bottomless alms bowl of eggplant verticillium wilt further include: is described more for holding the apparatus for placing (3) of bacteria suspension
Row nutritive cube (1) is set in the apparatus for placing (3) by the nutritive cube bracket (2).
3. a kind of bottomless alms bowl for eggplant verticillium wilt according to claim 1 hurts root classification inoculation apparatus, which is characterized in that institute
The front end for stating the first connection wing (13) is inwardly formed recess, and the front end of second connection wing (14) is outwardly formed protrusion, described
Recess and described project through are clamped connection.
4. a kind of bottomless alms bowl for eggplant verticillium wilt according to claim 1 hurts root classification inoculation apparatus, which is characterized in that institute
State the first side plate (11), second side plate (12), the bottom plate (15), first connection wing (13) and second connection
The wing (14) is integrally formed, and is additionally provided with multiple grooves (17) on first side plate (11) and second side plate (12).
5. a kind of bottomless alms bowl for eggplant verticillium wilt according to claim 1 hurts root classification inoculation apparatus, which is characterized in that institute
Stating nutritive cube bracket (2) includes: backing board (21) and muti-piece coaming plate (22), and the coaming plate (22) can revolve with the backing board (21)
The connection turned, the connection fixed by snap of adjacent coaming plate.
6. a kind of bottomless alms bowl for eggplant verticillium wilt according to claim 1 hurts root classification inoculation apparatus, which is characterized in that more
Arranging nutritive cube (1) is eight rows, the first side plate (11) of each row's nutritive cube, the second side plate (12), the first connection wing (13) and
The second connection wing (14), which is surrounded, is separated into four individual kind of plant holes for the nutritive cube (1) of each row.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820858724.3U CN208446153U (en) | 2018-06-04 | 2018-06-04 | A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820858724.3U CN208446153U (en) | 2018-06-04 | 2018-06-04 | A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus |
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| Publication Number | Publication Date |
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| CN208446153U true CN208446153U (en) | 2019-02-01 |
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|---|---|---|---|
| CN201820858724.3U Expired - Fee Related CN208446153U (en) | 2018-06-04 | 2018-06-04 | A kind of bottomless alms bowl for eggplant verticillium wilt hurts root classification inoculation apparatus |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108718771A (en) * | 2018-06-04 | 2018-11-02 | 黑龙江省农业科学院园艺分院 | It is a kind of to hinder root inocalation method progress eggplant verticillium wilt Resistance Identification method using bottomless alms bowl |
-
2018
- 2018-06-04 CN CN201820858724.3U patent/CN208446153U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108718771A (en) * | 2018-06-04 | 2018-11-02 | 黑龙江省农业科学院园艺分院 | It is a kind of to hinder root inocalation method progress eggplant verticillium wilt Resistance Identification method using bottomless alms bowl |
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