CN115837206A - Liquid pumping device for DNA synthesizer and liquid pumping method thereof - Google Patents
Liquid pumping device for DNA synthesizer and liquid pumping method thereof Download PDFInfo
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- CN115837206A CN115837206A CN202211395291.XA CN202211395291A CN115837206A CN 115837206 A CN115837206 A CN 115837206A CN 202211395291 A CN202211395291 A CN 202211395291A CN 115837206 A CN115837206 A CN 115837206A
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Abstract
The invention discloses a liquid pumping device for a DNA synthesizer and a liquid pumping method thereof in the technical field of matching devices of the DNA synthesizer.
Description
Technical Field
The invention relates to the technical field of matched devices of DNA synthesizers, in particular to a liquid pumping device and a liquid pumping method for a DNA synthesizer.
Background
The DNA synthesizer comprises a porous synthesis plate and a plurality of carriers, wherein the plurality of carriers are arranged in a plurality of holes of the porous synthesis plate in a one-to-one correspondence manner. When synthesizing DNA, it is necessary to inject multiple reagents into each hole of the multi-hole synthesis plate according to a specific chemical reaction step, after the reagents are injected, the reagents flow to the carrier along the hole wall, so that the multiple reagents in each hole react in the carrier in sequence to obtain a compound, and after the reaction of the multiple reagents is finished, the waste liquid after the reaction needs to be discharged.
The mode of present discharge waste liquid is installation drawing liquid device on DNA synthesizer, drawing liquid device includes the collection liquid structure, the suction structure and collection structure, when not drawing liquid, the collecting well of collection liquid structure staggers with a plurality of holes of porous synthetic board, make a plurality of downthehole reagents by the unable outflow of collection liquid structure shutoff, thereby guarantee that a plurality of downthehole reagent reactions can normally go on, during the drawing liquid, the collecting well through the collection liquid structure communicates with a plurality of holes of porous synthetic board, make through suction and make every downthehole waste liquid inflow collection assembly, realize the purpose of the downthehole waste liquid of discharge.
However, the top of the liquid collecting structure is abraded due to the fact that the top of the liquid collecting structure needs to be staggered and communicated frequently, the liquid collecting structure cannot be attached to the porous synthetic plate after abrasion, reagent reaction and negative-pressure liquid suction are affected, the smell of the extracted waste liquid is seriously leaked, and harm is easily caused to a human body.
Disclosure of Invention
The present invention aims to provide a liquid extracting device for a DNA synthesizer and a liquid extracting method thereof, so as to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a liquid pumping device for a DNA synthesizer comprises a liquid collecting structure and a liquid collecting plate, wherein the top of the liquid collecting structure comprises a liquid collecting plate, the liquid collecting plate is attached to a porous synthesis plate on the DNA synthesizer, liquid collecting holes are uniformly formed in the liquid collecting plate, a transmission pipe communicated with the liquid collecting holes is uniformly arranged on the left side of the liquid collecting plate, sliding blocks are arranged on the front side and the rear side of the liquid collecting plate, sliding rails are connected to the bottoms of the sliding blocks in a sliding mode, supporting assemblies are arranged at the bottoms of the sliding rails, connecting plates are arranged on the sliding rails on the right side of the supporting assemblies and are provided with electric telescopic rods, and the output ends of the electric telescopic rods are connected with the liquid collecting plate;
a pressure pumping structure;
and (4) collecting the structure.
As a preferable embodiment of the drawing apparatus for a DNA synthesizer according to the present invention, wherein: the supporting component comprises a top plate connected with the bottom of the sliding rail, telescopic rods are arranged on two sides of the bottom of the top plate, the bottom end of each telescopic rod is connected with the bottom plate, the bottom of the bottom plate is arranged on the base, supporting springs are arranged on two sides of the top of the bottom plate, and the supporting springs are sleeved on the outer sides of the telescopic rods respectively.
As a preferable aspect of the drawing apparatus for a DNA synthesizer of the present invention, wherein: the pumping structure comprises a pumping pump arranged on the left side of the top of the base, a first hose is arranged at the input end of the pumping pump and communicated with a funnel, the funnel is communicated with a transmission pipe, and a second hose is arranged at the output end of the pumping pump.
As a preferable embodiment of the drawing apparatus for a DNA synthesizer according to the present invention, wherein: the hose II is connected with the inner ring of the bearing, and the bottom end of the outer ring of the bearing is connected with an internal threaded pipe.
As a preferable embodiment of the drawing apparatus for a DNA synthesizer according to the present invention, wherein: the collecting structure comprises a supporting seat arranged on a base, a collecting box is arranged on the supporting seat, a top opening of the collecting box is provided with a convex ring, the top of the convex ring is provided with an external threaded pipe matched with the internal threaded pipe, a cross frame penetrates through the top opening of the collecting box, a reset spring is connected between the cross frame and the collecting box, an inclined rod is hinged to the cross frame, the top end of the inclined rod is hinged to a vertical frame, and the vertical frame is connected to the collecting box in a sliding mode.
As a preferable embodiment of the drawing apparatus for a DNA synthesizer according to the present invention, wherein: and the middle upper part of the collecting box is provided with an activated carbon filtering frame.
As a preferable embodiment of the drawing apparatus for a DNA synthesizer according to the present invention, wherein: the bottom of the collecting box is inserted on the supporting seat.
A liquid extracting method of a liquid extracting device for a DNA synthesizer specifically comprises the following steps:
the method comprises the following steps: the collecting box is inserted and placed on the supporting seat, the internal thread pipe is taken, the internal thread pipe is rotatably installed on the external thread pipe, when the internal thread pipe is rotatably installed, the outer ring of the bearing rotates on the inner ring of the bearing, the hose II cannot be driven to rotate, and when the internal thread pipe is downwardly rotatably installed, the vertical frame is downwardly extruded, so that the vertical frame drives the transverse frame to move leftwards through the inclined rod, the opening at the top of the collecting box is opened, and the installation of the collecting structure is completed;
step two: when liquid is not pumped, liquid collecting holes in the liquid collecting plate are staggered with a plurality of holes in the porous synthetic plate on the DNA synthesizer, reagents in the holes are blocked by the liquid collecting plate and cannot flow out, the reactions of the reagents in the holes can be normally carried out, when liquid is pumped, the liquid collecting plate is made to transversely move through the work of an electric telescopic rod, the liquid collecting holes in the liquid collecting plate are made to be communicated with the holes in the porous synthetic plate, at the moment, waste liquid enters the liquid collecting holes through the work of a pumping and pressing pump, is further pumped in through a transmission pipe, a funnel and a hose I in sequence and then is discharged into a collecting box through a hose II, and the odor of the waste liquid is filtered and purified through an active carbon filter frame on the collecting box and then is discharged, so that the harm to a human body is reduced;
step three: after the top of the liquid collecting plate is worn, the top plate is driven to jack up the slide rail upwards through the elasticity of the supporting spring, so that the liquid collecting plate can be kept attached to the porous synthetic plate, and reagent reaction and negative-pressure liquid pumping are prevented from being influenced;
step four: when the waste liquid level will reach the position of active carbon filter frame in the collecting box, unscrew the internal thread pipe, erect this moment and no longer receive the extrusion of internal thread pipe, under reset spring's effect, drive the crossbearer and seal up the open-top of collecting box again to when taking off the collecting box, the open-top of collecting box can self-sealing, can further reduce the harm that waste liquid smell led to the fact the human body.
Compared with the prior art, the invention has the beneficial effects that: by arranging the supporting assembly, after the top of the liquid collecting plate is worn, the top plate is driven to jack up the slide rail upwards through the elasticity of the supporting spring, so that the liquid collecting plate can be kept attached to the porous synthetic plate, and reagent reaction and negative-pressure liquid pumping are prevented from being influenced; after the collecting structure is installed, the cross frame for sealing the top opening of the collecting box can be opened along with the collecting structure, so that waste liquid can enter the collecting box, and the activated carbon filter frame is arranged on the collecting box, so that odor on the waste liquid collected in the collecting box can be filtered, purified and discharged, and harm to a human body is reduced; when the collecting box is taken off, the internal thread pipe is unscrewed, the vertical frame is not extruded by the internal thread pipe any more, the horizontal frame is driven to reseal the top opening of the collecting box under the action of the reset spring, and therefore when the collecting box is taken off for transportation, the top opening of the collecting box can automatically reset and seal, and the harm of waste liquid smell to a human body can be further reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a liquid drawing device for a DNA synthesizer and a liquid drawing method thereof according to the present invention;
FIG. 2 is a schematic top view of a partial structure of an apparatus and a method for drawing a solution for a DNA synthesizer according to the present invention;
FIG. 3 is a schematic sectional view of the structure of a bearing and an internally threaded tube according to the liquid extracting device and the liquid extracting method for the DNA synthesizer of the present invention;
FIG. 4 is a schematic diagram of a collection structure of an apparatus and method for drawing a DNA synthesizer according to the present invention.
In the figure: 1. a liquid collecting plate; 2. a liquid collecting hole; 3. a conveying pipe; 4. a slider; 5. a slide rail; 6. a connecting plate; 7. an electric telescopic rod; 8. a top plate; 9. a telescopic rod; 10. a base plate; 11. a support spring; 12. a base; 13. a funnel; 14. an activated carbon filter frame; 15. a first hose; 16. a pumping pump; 17. a second hose; 18. a bearing; 19. an internally threaded tube; 20. a supporting seat; 21. a collection box; 22. a convex ring; 23. an externally threaded tube; 24. a cross frame; 25. a return spring; 26. a diagonal bar; 27. and (5) erecting.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1 to 4, a liquid suction device for a DNA synthesizer comprises a liquid collection structure, wherein the liquid collection structure comprises a liquid collection plate 1, the top of the liquid collection plate is attached to a porous synthetic plate on the DNA synthesizer, liquid collection holes 2 are uniformly formed in the liquid collection plate 1, the liquid collection holes 2 correspond to a plurality of holes in the porous synthetic plate, transmission pipes 3 communicated with the liquid collection holes 2 are uniformly formed in the left side of the liquid collection plate 1, waste liquid entering the liquid collection holes 2 is uniformly discharged through the transmission pipes 3, sliding blocks 4 are arranged on the front side and the rear side of the liquid collection plate 1, sliding rails 5 are slidably connected to the bottoms of the sliding blocks 4, transverse movement of the liquid collection plate 1 can be guided, supporting components are arranged at the bottoms of the sliding rails 5, the bottoms of the supporting components are arranged on a base 12, a connecting plate 6 is arranged on the sliding rails 5 on the right side, electric telescopic rods 7 are arranged on the connecting plate 6, the output ends of the electric telescopic rods 7 are connected with the liquid collection plate 1, and the electric telescopic rods 7 can drive the liquid collection plate 1 to transversely move;
a pressure pumping structure;
and (4) collecting the structure.
The supporting component includes roof 8 of being connected with 5 bottoms of slide rail, the bottom both sides of roof 8 all are provided with telescopic link 9, the bottom and the bottom plate 10 of telescopic link 9 are connected, the bottom setting of bottom plate 10 is on base 12, the top both sides of bottom plate 10 all are provided with supporting spring 11, and two sets of supporting spring 11 overlap respectively in the outside of two sets of telescopic link 9, the telescopic link is used for leading the removal to roof 8, through setting up the supporting component, after the wearing and tearing at 1 top of collection liquid board, elasticity through supporting spring 11, drive roof 8 jack-up slide rail 5 that makes progress, and then make collection liquid board 1 can keep with porous synthetic board laminating together, avoid influencing reagent reaction and negative pressure drawing liquid.
The pumping structure comprises a pumping pump 16 arranged on the left side of the top of a base 12, a first hose 15 is arranged at the input end of the pumping pump 16, the first hose 15 is communicated with a funnel 13, the funnel 13 is communicated with a transmission pipe 3, a second hose 17 is arranged at the output end of the pumping pump 16, the first hose 15 is arranged, the pumping pump 16 is not influenced when the funnel 13 moves along with a liquid collecting plate 1, the second hose 17 is arranged, and the pumping pump 16 is still not influenced when an internal thread pipe 19 is installed.
The second hose 17 is connected with the inner ring of the bearing 18, the bottom end of the outer ring of the bearing 18 is connected with an internal threaded pipe 19, and when the internal threaded pipe 19 is rotated, the outer ring of the bearing 18 rotates on the inner ring of the bearing 18 without driving the second hose 17 to rotate.
The collecting structure comprises a supporting seat 20 arranged on a base 12, a collecting box 21 is arranged on the supporting seat 20, a convex ring 22 is arranged at the top opening of the collecting box 21, an external threaded pipe 23 matched with the internal threaded pipe 19 is arranged at the top of the convex ring 22, the internal threaded pipe 19 can be installed on the external threaded pipe 23 in this way, a transverse frame 24 penetrates through the top opening of the collecting box 21, the transverse frame 24 is used for sealing the top opening of the collecting box 21, waste liquid smell is prevented from being discharged from the opening, a reset spring 25 is connected between the transverse frame 24 and the collecting box 21, an inclined rod 26 is hinged on the transverse frame 24, the top end of the inclined rod 26 is hinged on a vertical frame 27, the vertical frame 27 is connected onto the collecting box 21 in a sliding mode, the internal threaded pipe 19 can downwards extrude the vertical frame 27 when installed in a downward rotating mode on the external threaded pipe 23, the vertical frame 27 is guided by the collecting box 21, the inclined rod 26 drives the transverse frame 24 to move leftwards, the top opening of the collecting box 21 can be opened, and waste liquid can enter the collecting box 21 when liquid is pumped.
The middle upper part of the collecting box 21 is provided with the activated carbon filter frame 14, so that the middle lower part of the collecting box 21 can store waste liquid, and the odor of the sent waste liquid can be discharged after being filtered and purified by the activated carbon filter frame 14, thereby reducing the harm to human body.
Example 2
Referring to fig. 4, the bottom of the collecting box 21 is inserted into the supporting base 20, so that the collecting box 21 can be positioned, and the stability in use is improved.
The rest of the structure was the same as in example 1.
A liquid extracting method of an extracting device for a DNA synthesizer adopts the extracting device for the DNA synthesizer in embodiment 2, and comprises the following steps:
the method comprises the following steps: the collecting box 21 is inserted and placed on the supporting seat 20, the internal thread pipe 19 is taken, the internal thread pipe 19 is rotatably installed on the external thread pipe 23, when the internal thread pipe 19 is rotatably installed, the outer ring of the bearing 18 rotates on the inner ring of the bearing 18, the second hose 17 cannot be driven to rotate, and when the internal thread pipe 19 is rotatably installed downwards, the vertical frame 27 is downwards extruded, so that the vertical frame 27 drives the transverse frame 24 to move leftwards through the inclined rod 26, the opening at the top of the collecting box 21 is opened, and the installation of the collecting structure is completed;
step two: when liquid is not pumped, the liquid collecting holes 2 in the liquid collecting plate 1 are staggered with a plurality of holes in the porous synthetic plate on the DNA synthesizer, reagents in the holes are blocked by the liquid collecting plate 1 and cannot flow out, the reaction of the reagents in the holes can be normally carried out, when liquid is pumped, the liquid collecting plate 1 is transversely moved through the operation of the electric telescopic rod 7, the liquid collecting holes 2 in the liquid collecting plate 1 are communicated with the holes in the porous synthetic plate, at the moment, the pumping pump 16 works, waste liquid enters the liquid collecting holes 2 through the holes and then is pumped in through the transmission pipe 3, the funnel 13 and the hose I15 in sequence and then is discharged into the collecting box 21 through the hose II 17, the odor of the waste liquid is filtered and purified through the activated carbon filter frame 14 on the collecting box 21 and then is discharged, and the harm to a human body is reduced;
step three: after the top of the liquid collecting plate 1 is worn, the top plate 8 is driven to jack up the slide rail 5 upwards through the elasticity of the supporting spring 11, so that the liquid collecting plate 1 can be kept attached to the porous synthetic plate, and reagent reaction and negative-pressure liquid pumping are prevented from being influenced;
step four: when the waste liquid level is about to reach the position of active carbon filter frame 14 in collecting box 21, unscrew internal thread pipe 19, erect frame 27 and no longer receive internal thread pipe 19's extrusion this moment, under reset spring 25's effect, drive crossbearer 24 and seal the open-top of collecting box 21 again to when taking off collecting box 21 and transporting, the open-top of collecting box 21 can self-sealing, can further reduce the harm that waste liquid smell led to the fact the human body.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A draw liquid device for DNA synthesizer which characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
the liquid collection structure comprises a liquid collection plate (1) with the top part being attached to a porous synthesis plate on a DNA synthesizer, wherein liquid collection holes (2) are uniformly formed in the liquid collection plate (1), transmission pipes (3) communicated with the liquid collection holes (2) are uniformly arranged on the left side of the liquid collection plate (1), sliding blocks (4) are arranged on the front side and the rear side of the liquid collection plate (1), the bottoms of the sliding blocks (4) are respectively connected with a sliding rail (5) in a sliding manner, a supporting assembly is arranged at the bottom of each sliding rail (5), the bottom of each supporting assembly is arranged on a base (12), a connecting plate (6) is arranged on each sliding rail (5) on the right side, an electric telescopic rod (7) is arranged on each connecting plate (6), and the output end of each electric telescopic rod (7) is connected with the liquid collection plate (1);
a pressure pumping structure;
and (4) collecting the structure.
2. The drawing device for a DNA synthesizer according to claim 1, wherein: the supporting component comprises a top plate (8) connected with the bottom of a sliding rail (5), telescopic rods (9) are arranged on two sides of the bottom of the top plate (8), the bottom end of each telescopic rod (9) is connected with a bottom plate (10), the bottom of each bottom plate (10) is arranged on a base (12), supporting springs (11) are arranged on two sides of the top of each bottom plate (10), and the supporting springs (11) are sleeved in the outer sides of the telescopic rods (9) respectively.
3. The drawing device for a DNA synthesizer according to claim 1, wherein: the pumping structure comprises a pumping pump (16) arranged on the left side of the top of a base (12), a first hose (15) is arranged at the input end of the pumping pump (16), the first hose (15) is communicated with a funnel (13), the funnel (13) is communicated with a transmission pipe (3), and a second hose (17) is arranged at the output end of the pumping pump (16).
4. The drawing device for a DNA synthesizer according to claim 3, wherein: the second hose (17) is connected with the inner ring of the bearing (18), and the bottom end of the outer ring of the bearing (18) is connected with an internal threaded pipe (19).
5. The drawing device for a DNA synthesizer according to claim 4, wherein: the collecting structure comprises a supporting seat (20) arranged on a base (12), a collecting box (21) is arranged on the supporting seat (20), a convex ring (22) is arranged at the top opening of the collecting box (21), an external threaded pipe (23) matched with an internal threaded pipe (19) is arranged at the top of the convex ring (22), a cross frame (24) penetrates through the top opening of the collecting box (21), a reset spring (25) is connected between the cross frame (24) and the collecting box (21), an inclined rod (26) is hinged to the cross frame (24), the top end of the inclined rod (26) is hinged to a vertical frame (27), and the vertical frame (27) is connected to the collecting box (21) in a sliding mode.
6. The drawing instrument for a DNA synthesizer according to claim 5, wherein: the middle upper part of the collecting box (21) is provided with an active carbon filtering frame (14).
7. The drawing device for a DNA synthesizer according to claim 6, wherein: the bottom of the collecting box (21) is inserted on the supporting seat (20).
8. The method for drawing a liquid using the drawing apparatus for a DNA synthesizer according to any one of claims 1 to 7, wherein: the method comprises the following steps:
the method comprises the following steps: the collecting box (21) is placed on the supporting seat (20) in an inserted mode, the internal threaded pipe (19) is taken, the internal threaded pipe (19) is rotatably installed on the external threaded pipe (23), when the internal threaded pipe (19) is rotatably installed, the outer ring of the bearing (18) rotates on the inner ring of the bearing (18) and cannot drive the hose II (17) to rotate, and when the internal threaded pipe (19) is rotatably installed downwards, the vertical frame (27) is downwards extruded, so that the vertical frame (27) drives the transverse frame (24) to move leftwards through the inclined rod (26), the opening in the top of the collecting box (21) is opened, and the installation of the collecting structure is completed;
step two: when liquid is not pumped, liquid collecting holes (2) in the liquid collecting plate (1) are staggered with a plurality of holes in a porous synthetic plate on a DNA synthesizer, reagents in the holes are blocked by the liquid collecting plate (1) and cannot flow out, reagent reaction in the holes can be normally carried out, when liquid is pumped, the liquid collecting plate (1) is enabled to transversely move through an electric telescopic rod (7), the liquid collecting holes (2) in the liquid collecting plate (1) are enabled to be communicated with the holes in the porous synthetic plate, at the moment, the liquid is enabled to enter the liquid collecting holes (2) through the holes by working through a pumping-out pump (16), then the liquid is pumped in through a transmission pipe (3), a funnel (13) and a hose I (15) in sequence and then is discharged into a collecting box (21) through a hose II (17), and odor of the liquid is discharged after being filtered and purified through an active carbon filtering frame (14) on the collecting box (21), so that harm to a human body is reduced;
step three: after the top of the liquid collecting plate (1) is worn, the top plate (8) is driven to jack up the slide rail (5) upwards through the elasticity of the supporting spring (11), so that the liquid collecting plate (1) can be kept attached to the porous synthetic plate, and reagent reaction and negative-pressure liquid pumping are prevented from being influenced;
step four: when the waste liquid level is about to reach the position that the frame (14) was strained to the active carbon in collecting box (21), unscrew internal thread pipe (19), erect frame (27) this moment and no longer receive the extrusion of internal thread pipe (19), under reset spring's (25) effect, drive crossbearer (24) and seal up the open-top of collecting box (21) again, thereby when taking off collecting box (21) and transporting, the open-top of collecting box (21) can self-sealing, can further reduce the harm that waste liquid smell caused to the human body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211395291.XA CN115837206A (en) | 2022-11-09 | 2022-11-09 | Liquid pumping device for DNA synthesizer and liquid pumping method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211395291.XA CN115837206A (en) | 2022-11-09 | 2022-11-09 | Liquid pumping device for DNA synthesizer and liquid pumping method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115837206A true CN115837206A (en) | 2023-03-24 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211395291.XA Withdrawn CN115837206A (en) | 2022-11-09 | 2022-11-09 | Liquid pumping device for DNA synthesizer and liquid pumping method thereof |
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| Country | Link |
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| CN (1) | CN115837206A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116474652A (en) * | 2023-06-21 | 2023-07-25 | 杭州联川生物技术股份有限公司 | Synthetic board waste liquid discharge device and gene synthesizer |
-
2022
- 2022-11-09 CN CN202211395291.XA patent/CN115837206A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116474652A (en) * | 2023-06-21 | 2023-07-25 | 杭州联川生物技术股份有限公司 | Synthetic board waste liquid discharge device and gene synthesizer |
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Application publication date: 20230324 |
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