CN115837050B

CN115837050B - Application of vesicle grass processed product

Info

Publication number: CN115837050B
Application number: CN202111103070.6A
Authority: CN
Inventors: 包明兰; 吴光华
Original assignee: Inner Mongolia University for Nationlities
Current assignee: Inner Mongolia University for Nationlities
Priority date: 2021-09-18
Filing date: 2021-09-18
Publication date: 2024-01-30
Anticipated expiration: 2041-09-18
Also published as: CN115837050A

Abstract

The invention provides an application of a vesicle grass processed product; the preparation method of the vesicle grass processed product comprises the following steps: step 1, mixing a vesicular herbal material with milk, soaking and drying to obtain a semi-processed product; grinding the semi-processed product, placing the semi-processed product into a conical flask, adding methanol, performing ultrasonic treatment, and filtering. The Mongolian medicine preparation for treating migraine has little toxic and side effect on human body, the medicine source is pure green plants, the injury to the body is little, and the treatment effect is obvious.

Description

Application of vesicle grass processed product

Technical Field

The invention belongs to the field of national medicines; in particular to the application of a vesicle grass processed product.

Background

Migraine is a genetically related chronic neurovascular disease, which mostly occurs in people of ages 25-55 years, and is often accompanied by symptoms such as nausea, vomiting, photophobia, etc. The disease is characterized by recurrent attacks, and severe pain can occur during attacks, which seriously affects the quality of life of the patient. Migraine can lead to reduced cognitive function in addition to the damage that the disease itself has. There are many pathogenesis leading to migraine, one theory of which suggests that increased sensitivity to migraine pain is caused by increased sensitivity to sensory perivascular fibers of the trigeminal vasculature, i.e., neurogenic inflammation. Because of this increased sensitivity, vascular pulsations that are not normally able to induce pain sensation become a strong pain stimulus and cause migraine with pulse pulsations. Neuropeptides such as substance P, neurokinin a and CGPR (peptides related to the calcitonin gene) that can induce neuronal inflammation are secreted from the nerve end. Wherein CGPR is detected upon migraine attacks for indicating the extent of increase in venous blood of the head. In addition, there is metabolism of 5-hydroxytryptamine during migraine pain.

Currently, no medicine specially used for treating migraine is clinically available, and when the treatment of mild migraine is to use non-morphine analgesics and the like, the treatment of moderate and severe migraine is to use non-morphine analgesics, antiemetics and the like in combination for treating migraine is generally recommended. However, long-term administration may cause damage to the liver and kidneys, with side effects, and is not possible.

In the theory of traditional Chinese medicine, migraine belongs to the category of headache and headache, and the research is carried out on five types of symptoms of liver-yang hyperactivity, turbid phlegm, blood stasis, kidney deficiency and deficiency of qi and blood.

The vesicular grass is a dried root of the plant vesicular grass Physosiphon (L.) of the Solanaceae family, G.don. Tibetan name is Shang Puru. Many Mongolian medicine classical works have been described in the literature. The ancient books describe that the vesicular grass is colored white Shang Puru, black Shang Puru and Huang Shangpu. However, these three kinds of vesicular grass are not one herb. Huang Shangpu is, for example, henbane, bai Shangpu is, for example, the current vesicular grass. It was collected in the 1998 department of health of the people's republic of China (Mongolian medicine division) and has the efficacy of killing "sticky", detumescence, disinsection, analgesia, jie Jing, clearing "Xueri Wusu", strengthening yang and the like. Is often used for viscous stomach-scraping, throat-forming, complications, insect diseases, cerebral stinging, headache and impotence. The vesicular grass is mainly produced in the inner Mongolian tin Lin Guole allied Abagaflag, hulun Bei Ermeng Ewenkeqi and Ke Shi City. In recent years, there are also vesicular plants in the tin Lin Guole allied western Wu Zhumu. Researches show that the main active ingredients of the current vesicular grass are anisodamine, hyoscyamine, scopolamine, red palustrine and atropine. The effective components have the effects of relieving asthma and cough, and tranquilizing. Since the vesicular herb is toxic, the Mongolian medicine usually adopts the following modes: (1) stir-frying with slow fire to yellow; (2) soaking in goat milk, and stewing thoroughly in plant ash; (3) soaking in milk and then sun-drying; (4) Decocting with water, and making into paste.

The preparation method comprises parching herba Pogostemonis with slow fire to brown. The method for soaking and processing sheep milk comprises soaking vesicle grass tablet in sheep milk for 4 hr, taking out, placing for 1-2 hr, wrapping with grass paper for 2-3 layers, slightly sprinkling clear water to wet, embedding into hot plant ash, and soaking to dry. The preparation method of the vesicular grass comprises the steps of taking vesicular grass slices, adding fresh milk in a certain proportion, and placing in a drying box at 60 ℃ for 4-6 hours. The paste preparation method is to take the whole herb of the vesicular herb to decoct with water, and prepare the paste for standby. At present, the chemical composition and pharmacodynamics research of the vesicle grass processed products (milk and ointment) are limited to the content measurement of total alkaloids and the anti-inflammatory, analgesic and antibacterial effects. The toxicity and pharmacological actions of the vesicular herb are reduced to different degrees after the vesicular herb is processed by the above 4 methods, and the toxicity of the processed product is the lowest after the vesicular herb is soaked in milk. Pharmacological experiments such as anti-inflammatory, analgesic, anti-fatigue and cough relieving are carried out on different processed products. The results show that the large, medium and small dosage groups of the vesicular herb have the effects of resisting inflammation, easing pain, resisting fatigue and relieving cough with different degrees.

Disclosure of Invention

The invention aims to provide the application of a vesicle grass preparation product.

The invention is realized by the following technical scheme:

the invention relates to application of a vesicle grass processed product in preparation of a medicine for treating migraine.

Preferably, the preparation method of the vesicle grass preparation product comprises the following steps:

step 1, mixing the vesicular herbal medicine with milk, soaking and drying to obtain a semi-processed product;

grinding the semi-processed product, placing the semi-processed product into a conical flask, adding methanol, performing ultrasonic treatment, and filtering.

Preferably, in the step 1, the mass ratio of the vesicle herbal medicine to the milk is 1:4.5.

Preferably, in step 1, the soaking time is 6 hours.

Preferably, in step 1, the temperature of the drying is 40 ℃.

Preferably, in step 1, the drying time is 4.5 hours.

Preferably, in step 2, the grain size of the half processed product after grinding is 60 mesh.

Preferably, in step 2, the half-finished product is included in an amount of 1.0g.

Preferably, in step 2, the volume of methanol added is 10ml-25ml.

Preferably, in step 2, the time of the ultrasonic treatment is 30-40 minutes, and the filtration membrane is a 0.22 μm microporous membrane.

The invention has the following advantages: the Mongolian medicine preparation for treating migraine has little toxic and side effect on human body, the medicine source is pure green plants, the injury to the body is little, and the treatment effect is obvious.

Drawings

FIG. 1 is a UPLC-MS diagram of a vesicular herbal medicine;

FIG. 2 is a UPLC-MS diagram of a vesicular herb preparation;

FIG. 3 is a UPLC-MS spectrum of hyoscyamine;

FIG. 4 is a UPLC-MS spectrum of scopolamine;

FIG. 5 is a UPLC-MS spectrum of anisodamine;

FIG. 6 is a graph comparing the effect of a vesicular herb preparation on NO in migraine rat serum;

FIG. 7 is a graph comparing the effect of a vesicular herb preparation on CGRP in serum of migraine rats;

FIG. 8 is a graph comparing the effect of a vesicular herb preparation on 5-HT in brain tissue of a migraine rat;

FIG. 9 is a graph comparing the effect of a vesicular herb preparation on beta-EP in brain tissue of migraine rats.

Detailed Description

The present invention will be described in detail with reference to specific examples. It should be noted that the following examples are only further illustrative of the present invention, but the scope of the present invention is not limited to the following examples.

Example 1

The present example relates to the use of a vesicular herb preparation for the preparation of a medicament for the treatment of migraine.

The preparation method of the vesicle grass processed product comprises the following steps:

The specific information of the raw materials involved in this example is as follows:

herba Buddha (inner Mongolian Hulun Bei Ershi Ke Shi Ling, inner Mongolian national university Mongolian medical college Bos He Bate mol teacher identified as eggplant family drug, lot number 20180726;

scopolamine (lot number 51-34-3, content > 98%) control (supplied by Efava biosciences technologies Co., ltd.).

Hyoscyamine (lot number: 101-31-5, content > 98%) control (supplied by Efava biosciences technologies Co., ltd.).

Anisodamine (batch No. 17659-49-3, content > 98%) control (supplied by Efava bioscience technology Co., ltd.).

Methanol (Tianjin Deen chemical Co., ltd., lot 20190318).

Triethylamine (Tianjin metallocene chemical reagent plant).

Acetonitrile (Fisher SeientificInc. United States)

1. Experimental instrument

Ultra-high performance liquid chromatography-tandem mass spectrometry (Waters Acquity UPLC System. Waters, inc.). A thermostat water bath (Jiangsu city instruments Co., ltd.) is shown. A 50ml conical flask, a 10ml graduated cylinder, a 20ml graduated cylinder, filter paper, a funnel, and a 1.5ml sample injection flask.

2. Preparation method and results

2.1 chromatographic conditions

Chromatographic column: liquid phase: triethylamine: acetonitrile, flow rate: 0.3ml/min column temperature: 35 ℃. Absorbance: 215nm. The sample injection amount was 5. Mu.L, as shown in the liquid phase control in Table 1.

TABLE 1

Number of times	1	2	3	4
					Time	0-4	4-15	15-15:10	15:10-16:00
Triethylamine	98-80	80-80	80-95	95-95
					Acetonitrile%	5-20	20-20	20-5	5-5

2.2 preparation of milk products

Step 1, mixing the vesicular herbal medicine and milk in a mass ratio of 1:4.5, soaking for 6 hours, and drying at 40 ℃ for 4.5 hours to obtain a semi-processed product;

step 2, preparing a milk processed product sample: grinding the semi-processed product, sieving with 60 mesh sieve, precisely weighing 1.0g, placing in conical flask, adding 25ml methanol solution, measuring weight, ultrasonic treating for 40 min, measuring weight, supplementing weight with methanol, and shaking and filtering. Again, 2ml of the solution is sucked up, placed in a 5ml graduated flask, graduated by adding methanol and evenly shaken. The solution was filtered through a 0.22 μm microporous filter, then precisely aspirated through 10 μm microporous filter, and placed in a mass spectrometer.

2.3 preparing a control solution

Hyoscyamine control solution: accurately weighing scopolamine reference substance 1.0mg, placing into a 10ml graduated flask, diluting to scale in methanol, and shaking.

Anisodamine control solution: accurately weighing anisodamine reference substance 1.0mg, placing into a 10ml graduated flask, diluting to scale in methanol, and shaking.

Scopolamine control solution: accurately weighing scopolamine reference substance 1.0mg, placing into a 10ml graduated flask, diluting to scale in methanol, and shaking.

The chemical composition changes of the vesicle preparation and the vesicle herbal drug prepared in this example are shown in Table 2.

Wherein UPLC-MS spectrum of the vesicular herbal medicine in Table 2 is shown in FIG. 1; the UPLC-MS spectrum of the vesicle grass processed product prepared in the example is shown in figure 2; wherein, the hyoscyamine UPLC-MS spectrum referred in Table 2 is shown in figure 3; the scopolamine UPLC-MS spectrum referred to in Table 2 is shown in FIG. 4; the UPLC-MS spectrum of anisodamine referred in Table 2 is shown in FIG. 5.

The peak area comparison of the chemical components of the vesicular herbal medicine and the processed product is shown in table 2.

TABLE 2

From table 2, it can be seen that the peak areas of the chemical components No. 1, 2, 3, 4, 5 and 6 are significantly reduced, the peak areas of the chemical components No. 8 and 9 are significantly increased, and the peak areas of the chemical components No. 11 and 12 are not shown after processing. The chemicals 7 and 10 of the milk products are novel compounds. 4. Scopolamine, anisodamine No. 5, 6.

The herb of Buddha is toxic and is used as a medical preparation. The main active ingredients of the vesicular grass are chemical ingredients such as hyoscyamine, scopolamine, anisodamine and the like, and the chemical ingredients are toxic ingredients of the vesicular grass. In the early experiments, the optimal process for processing the vesicular herb is selected, and the methods of milk soaking, goat milk soaking, stir-frying and the like known by the toxicity effect experiments of different processing methods can reduce the toxicity to different degrees. It is known that milk infusions have minimal toxicity and significant effects. The toxicity reduction of the milk processed vesicular herb is possibly related to the chemical products 1, 2, 3, 4, 5 and 6 in the experimental results. The anti-inflammatory pharmacological action may be related to the chemical components No. 8 and No. 9.

Example 2

The example relates to pharmacological effect research of the vesicular herb preparation on the prevention of migraine caused by nitroglycerin in rats, and is specifically as follows:

step 1, preparation of a drug solution: pulverizing herba Buddlejae processed product, dissolving with 0.5% sodium carboxymethylcellulose, and preparing into medicinal solution;

step 2, copying the molding: the method comprises the steps of taking 20 rats, randomly dividing the rats into a blank group and a model group, adding an equivalent amount of physiological saline into the blank group, taking brain tissues after neck injection of GNT 10mg/kg-1 45min into the model group, taking blood from an aorta, centrifuging to obtain serum, and placing the serum at the temperature of-80 ℃ for later use. According to the description of the kit, serum NO, CGRP, brain tissue 5-HT and beta-EP are detected, and meanwhile, the behavior indexes such as the number of times of climbing the cage and bending the head of the rat are observed.

Step 3, 60 SD rats weighing 180+ -20 g are randomly selected into a blank group, a model group, a positive control group, a high-dose group of the milk vesicle grass, a medium-dose group of the milk vesicle grass and a low-dose group of the milk vesicle grass. The blank group is given with normal saline, the positive control group is given with headache treating tablet, the high, medium and low dose components of the vesicular grass prepared from milk are respectively given with corresponding medicine solutions, the medicine solutions are continuously administered for 14 days once a day, and after the last administration, the neck is injected with nitroglycerin for molding, anesthesia, aortic blood taking and brain tissue taking are carried out, and the medicine solutions are placed at the temperature of minus 80 ℃ for standby. According to the description of the kit, serum NO, CGRP, brain tissue 5-HT and beta-EP are detected, and meanwhile, the behavior indexes such as the number of times of climbing the cage and bending the head of the rat are observed.

Experimental results: see FIG. 6 for a comparison of the effect of nigella sativa on NO in migraine rat serum; FIG. 7 is a graph comparing the effect of nigella sativa on CGRP in serum of migraine rats; FIG. 8 is a graph comparing the effect of nigella sativa on 5-HT in migraine rat brain tissue; FIG. 9 is a graph comparing the effect of vesicular grass on beta-EP in migraine rat brain tissue.

The Mongolian medicine preparation for treating migraine has little toxic and side effect on human body, the medicine source is pure green plants, the injury to the body is little, and the treatment effect is obvious.

The foregoing describes specific embodiments of the present invention. It is to be understood that the invention is not limited to the particular embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the claims without affecting the spirit of the invention.

Claims

1. The use of a vesicular herb preparation, characterized in that the vesicular herb preparation is used for the preparation of a medicament for treating migraine;

grinding the semi-processed product, placing the semi-processed product into a conical flask, adding methanol, performing ultrasonic treatment, and filtering;

in the step 1, the mass ratio of the vesicular herbal medicine to the milk is 1:4.5, the soaking time is 6 hours, the drying temperature is 40 ℃, and the drying time is 4.5 hours;

in the step 2, the grain diameter of the ground semi-processed product is 60 meshes, the calculated amount of the semi-processed product is 1.0g, the volume of methanol added is 10ml-25ml, the ultrasonic treatment time is 30-40 minutes, and the filtered filter membrane is a microporous filter membrane with the size of 0.22 mu m.