CN114767760B

CN114767760B - A composition with liver protecting effect

Info

Publication number: CN114767760B
Application number: CN202210467292.4A
Authority: CN
Inventors: 孙静; 张小飞
Original assignee: Shaanxi University of Chinese Medicine
Current assignee: Shaanxi University of Chinese Medicine
Priority date: 2022-04-02
Filing date: 2022-04-29
Publication date: 2023-07-25
Anticipated expiration: 2042-04-29
Also published as: CN114767760A

Abstract

The invention discloses a composition with liver protection effect, which is prepared from the following raw materials in parts by weight: 1 to 2 parts of kudzuvine root and 1 to 1.5 parts of shizandra berry. The composition with the liver protection function has the advantages of remarkable liver protection effect, low cost, good effect and practical popularization and application value by combining the shizandra berry and the kudzuvine root in a specific proportion and using the simple medicinal composition.

Description

A composition with liver protecting effect

Technical Field

The invention particularly relates to a composition with a liver protection effect.

Background

Alcoholic liver disease is a liver injury disease caused by long-term drinking of a large amount, and the incidence rate of alcoholic liver disease in recent years in China rises year by year, and is the second largest factor causing liver disease. Research shows that excessive drinking for a long time can cause injury of important organs of human body such as liver, intestines and stomach, cardiovascular, endocrine, central nervous system and the like, wherein oxidative stress, inflammation and the like induced by alcohol metabolism are main causes of the pathogenesis of alcoholic liver diseases.

The schisandra chinensis is a dry mature fruit of schisandra chinensis Schisandra chinensis of magnoliaceae vine, has long administration history, is an important liver-protecting traditional Chinese medicine since ancient times, and modern pharmacological researches show that the schisandra chinensis extract and the active ingredients thereof have various liver-protecting effects, have good prevention and treatment effects on various liver diseases such as drug-induced liver injury, liver cirrhosis, liver failure and the like, and have various pharmacological activities such as enzyme reduction, liver protection, antioxidation, cytochrome P450 oxidase regulation, hepatocyte apoptosis inhibition and the like.

The radix Puerariae is dry root of Leguminosae plant or Gangerina plant, has long administration history, and can be used for treating fever, headache, inflammation, etc. The traditional Chinese medicine holds that the excessive drinking of wine is toxic, and can cause the organism to block the qi movement and damp and generate heat. Pueraria has the effects of promoting salivation, quenching thirst, relieving exterior syndrome, reducing fever, and expelling pathogenic fire. Pharmacological research indicates that the kudzuvine root tea contains carbohydrate, amino acid, mineral substances and the like, has good antioxidation effect and can exert excellent anti-alcohol capability. The kudzuvine root also contains a large amount of flavonoid compounds, and the flavonoid can repair the liver subjected to pathological damage and reduce glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase in serum, so that the occurrence of cardiovascular adverse conditions caused by patients is reduced, and the alcoholic liver injury can be treated.

Patent CN106665976A discloses kudzuvine root anti-alcoholic tea which is prepared by compounding two medicines of shizandra berry and kudzuvine root, only plays the role of kudzuvine root in refreshing brain and dispelling alcohol, and has no definite curative effect on protecting liver; patent CN110876772A discloses a traditional Chinese medicine preparation with liver protecting function, wherein the medicinal ingredients comprise semen Hoveniae and ginseng besides shizandra berry and kudzuvine root, and the medicinal ingredients are more, high in cost and unfavorable for practical popularization and application.

Disclosure of Invention

In order to solve the problems, the invention provides a composition with liver protection effect, which is prepared from the following raw materials in parts by weight:

1 to 2 parts of kudzuvine root and 1 to 1.5 parts of shizandra berry.

Further, the traditional Chinese medicine is prepared from the following raw materials in parts by weight:

1.5 to 2 parts of kudzuvine root and 1 part of Chinese magnoliavine fruit.

1.5 parts of kudzuvine root and 1 part of Chinese magnoliavine fruit.

Further, the preparation is prepared by taking medicinal powder of the raw material medicine or water or organic solvent extract of the raw material medicine as an active ingredient and adding pharmaceutically acceptable auxiliary materials.

Still further, the formulation is an oral formulation.

Further, the oral preparation is granule, paste, powder, pill or solution.

The invention also provides a preparation method of the pharmaceutical composition, which comprises the following steps:

(1) Weighing the raw materials according to the proportion;

(2) Grinding the raw materials into powder, or extracting with water or organic solvent, and adding adjuvants or auxiliary components commonly used in medicine.

The invention finally provides application of the composition in preparing a medicament for treating liver injury.

Further, the medicament is a medicament for treating alcoholic liver injury.

Animal experiments show that the radix puerariae and schisandra chinensis with different compatibility ratios have different liver protection effects, wherein when the radix puerariae: when the compatibility of the schisandra chinensis is 1.5:1, the liver protection effect is most obvious, and the ALDH content in liver tissues is obviously increased compared with that in a model group.

The composition with the liver protection function has the advantages of remarkable liver protection effect, low cost, good effect and practical popularization and application value by combining the shizandra berry and the kudzuvine root in a specific proportion and using the simple medicinal composition.

It should be apparent that, in light of the foregoing, various modifications, substitutions and alterations can be made herein without departing from the spirit and scope of the invention as defined by the appended claims.

The above-described aspects of the present invention will be described in further detail below with reference to specific embodiments in the form of examples. It should not be understood that the scope of the above subject matter of the present invention is limited to the following examples only. All techniques implemented based on the above description of the invention are within the scope of the invention.

Drawings

FIG. 1 shows a graph of liver pathology and liver function index change in rats

Fig. 2HE staining results (a: blank b: model c: kudzuvine root d: schisandra chinensis e kudzuvine root: five=2:1f: kudzuvine root: five=1.5:1g: kudzuvine root: five=1:1h: kudzuvine root: five=1:1.5i: khhgp)

Detailed Description

EXAMPLE 1 preparation of the composition of the invention

The formula comprises the following components: 1.5 parts of kudzuvine root and 1 part of Chinese magnoliavine fruit.

The preparation method comprises the following steps: weighing the raw materials according to the proportion, adding 10 times of water, decocting for 2 times each for 30 minutes, filtering, combining the filtrates, concentrating the filtrate under reduced pressure to obtain paste, adding common auxiliary materials in medicines, and uniformly stirring.

EXAMPLE 2 preparation of the composition of the invention

The formula comprises the following components: 2 parts of kudzuvine root and 1 part of Chinese magnoliavine fruit.

EXAMPLE 3 preparation of the composition of the invention

The formula comprises the following components: 1 part of kudzuvine root and 1 part of Chinese magnoliavine fruit.

EXAMPLE 4 preparation of the composition of the invention

The formula comprises the following components: 1 part of kudzuvine root and 1.5 parts of Chinese magnoliavine fruit.

The advantageous effects of the present invention are described below by way of test examples.

Experimental example 1 study of influence of Pueraria lobata and Schisandra chinensis on rats with alcoholic liver injury

Preparation of (I) medicine

Decocting fructus Schisandrae chinensis, radix Puerariae, and radix Puerariae with 10 times of water for 30min, extracting twice, mixing the two decoctions, evaporating to dryness, and concentrating to obtain extract. ( And (3) injection: the unit parts of the Chinese magnoliavine root correspond to the dosages of the Chinese magnoliavine root which are independently administered respectively )

(II) administration and grouping

Healthy SPF-grade SD rats were randomly divided into 8 groups of 8 animals after 3d of adaptive rearing. The method comprises the following steps of: blank group, model group, shan Wuwei sub-administration group (1.0 g/kg), single radix Puerariae administration group (1.6 g/kg), shizandra berry and radix Puerariae compatibility 2:1 administration group (1.6 g/kg radix Puerariae+0.5 g/kg fructus Schisandrae chinensis), fructus Schisandrae chinensis radix Puerariae compatibility 1.5:1 administration group (1.2 g/kg radix Puerariae+0.5 g/kg fructus Schisandrae chinensis), fructus Schisandrae chinensis radix Puerariae compatibility 1:1 administration group (0.8 g/kg radix Puerariae+0.5 g/kg fructus Schisandrae chinensis), fructus Schisandrae chinensis radix Puerariae compatibility 1:1.5 administration group (0.8 g/kg radix Puerariae+0.75 g/kg fructus Schisandrae chinensis), positive drug (sunflower liver-protecting tablet KHHGP 0.4375 g/kg). Each group was administered with 0.9% physiological saline or drug by gavage, and after 1h, 56℃Erguotou was administered at an amount of 10mL/kg for 14 consecutive days. ( And (3) injection: in order to eliminate the influence caused by the overlarge gap between unit administration and compatible administration doses, the doses of the radix puerariae and the Shan Wuwei are doubled, so that the treatment effects among the groups are better compared. )

(III) detection of physiological and biochemical indicators of rats

After the last time of drug infusion for 1h, anesthesia is carried out, blood is taken from abdominal aorta, serum is separated after centrifugation for 15min at 4 ℃ and 5000r/min, liver is taken, surface blood trace is cleaned by washing with precooled normal saline, surface moisture is absorbed by filter paper, the weight of the liver is weighed, the liver index is calculated, and the liver index is calculated according to the following formula:

liver index (%) =liver mass (g)/body mass (g) ×100%

According to the operation of the instruction book of the kit, the ALT and AST contents in serum are measured, 0.2g of left lobe tissue of liver is added with 9 times of 0.9% physiological saline to prepare 10% liver tissue homogenate, and the homogenate is centrifuged at 12000r/min for 10min to collect supernatant for standby. ELISA kit for measuring ALDH activity in liver tissue.

Fixing liver tissue with 10% neutral paraformaldehyde for 24 hr, dewatering in 70% alcohol solution for 24 hr, embedding with conventional paraffin to obtain slice with thickness of about 4 μm, dewaxing with C8H10, and dewatering with alcohol gradient (95%, 90%, 80%, 75%); hematoxylin staining; alcohol differentiation of hydrochloric acid; conventional gradient alcohol (75%, 80%, 90%, 95%) after 0.5% eosin staining was dehydrated with 100% ethanol (10 min), 100% alcohol 1 (10 min); finally, the neutral resin sealing sheet is transparent through dimethylbenzene, and different visual fields are selected for photographing and analyzing the histopathological changes of the liver under a Nikon optical microscope Eclipse Ci-L.

2. Experimental results

The test results of the kit are shown in Table 1 and FIG. 1, and as can be seen from Table 1 and FIG. 1, the liver index of the Model group (Model) is obviously increased compared with that of the blank group (Control), and P is less than 0.05, so that the Model group (Model) has statistical significance. After administration, liver index of each group decreased to a different extent. Analysis of related pathological biochemical indexes shows that when the kudzuvine root is: when the ratio of the shizandra berry to the Chinese magnoliavine fruit is 1.5:1, the ALT and AST contents in serum are obviously reduced compared with the model group, and the contents are obviously different. Compared with a positive medicine (KHHGP sunflower liver-protecting tablet) with a rather clinical dosage, the Chinese medicinal composition has better treatment and protection effects. The obvious effect of the compatibility of medicines is shown. Meanwhile, the activity of acetaldehyde dehydrogenase ALDH can be obviously improved, and the compatibility of the kudzuvine root and the schisandra chinensis can be primarily judged to have a better protection effect on rat liver injury caused by alcohol.

TABLE 1 liver function index difference table for rat liver

*p＜0.05，**p＜0.01，***p＜0.001

HE staining results are shown in FIG. 2, and the blank group of liver cells are loose in structure, a small amount of liver cells are slightly edema, and no obvious inflammatory cell infiltration is seen in tissues as shown in FIG. 2 a; model group fig. 2b liver tissue overall structure is slightly abnormal, the tissue can be seen to be obviously hyperemic and dilated, and the tissue is infiltrated by a small amount of inflammatory cells; single kudzuvine root group fig. 2c, shan Wuwei subgroup fig. 2d, kudzuvine root: schisandra = 2:1 dosing group figure 2e, kudzuvine root: schisandra = 1:1 dosing group figure 2g and kudzuvine root: schisandra = 1:1.5 dosing group figure 2h tissue overall structure is substantially normal, liver cell structure is full, liver sinuses are arranged radially along central vein, and no obvious inflammatory cell infiltration is seen in the tissue. Radix Puerariae: schisandra = 1.5:1 dosing group figure 2f liver tissue overall structure basic group is normal, liver cell structure is full, central vein is clearly visible, liver sinuses are arranged radially along central vein, tissue can be seen to expand liver Dou Qingwei, and tissue is not seen to be significantly infiltrated by inflammatory cells. The results of the data of the kit are shown in fig. 1 and table 1, and it can be seen from fig. 1 and table 1 that the Model group (Model) has significantly higher liver index than the blank group (Control), and P <0.05 has statistical significance, and the liver index of each group has different degree of decrease after administration. Analysis of related pathological biochemical indexes shows that when the kudzuvine root is: when the ratio of the shizandra berry to the mixture is 1.5:1, the content of ALT and AST in serum is obviously reduced, and meanwhile, the activity of acetaldehyde dehydrogenase ALDH can be obviously improved, and the mixture is judged to have a better protection effect on the large tertiary liver injury caused by alcohol in the first step.

3. Discussion of the invention

According to the invention, a rat model of alcoholic liver injury is obtained by infusing stomach with alcohol to replicate alcoholic liver injury, the liver protection effect of different compatibility of schisandra chinensis and pueraria lobata is observed, the liver protection effect of the pueraria lobata and schisandra chinensis with different compatibility ratios is different through monitoring and analysis of related biochemical indexes of rat serum and liver tissue, and when the pueraria lobata: when the compatibility of the schisandra chinensis is 1.5:1, the liver protection effect is most obvious, the ALDH content in liver tissues is obviously increased compared with that of a model group, and the schisandra chinensis has the obvious liver protection effect.

In conclusion, the composition with liver protection function has the advantages of remarkable liver protection function, low cost, good effect and practical popularization and application value by combining the shizandra berry and the kudzuvine root in a specific proportion and using the simple medicinal flavor composition.

Claims

1. A composition with liver protection effect, which is characterized in that: the traditional Chinese medicine is prepared from the following raw materials in parts by weight:

1.5 parts of kudzuvine root and 1 part of Chinese magnoliavine fruit.

2. The composition of claim 1, wherein: the preparation is prepared by taking medicinal powder of the raw materials or water or organic solvent extracts of the raw materials as active ingredients and adding pharmaceutically acceptable auxiliary materials.

3. The composition of claim 2, wherein: the preparation is an oral preparation.

4. A composition according to claim 3, characterized in that: the oral preparation is granule, paste, powder, pill or solution.

5. The method for preparing the pharmaceutical composition according to any one of claims 1 to 4, characterized in that: it comprises the following steps:

(1) Weighing the raw materials according to the proportion of claim 1;

6. Use of the composition according to any one of claims 1 to 5 for the preparation of a medicament for the treatment of liver injury.

7. The composition of claim 6, wherein: the medicine is used for treating alcoholic liver injury.