CN108272941A

CN108272941A - A kind of Chinese medicine preparation and preparation method thereof for treating chronic chyluria

Info

Publication number: CN108272941A
Application number: CN201810108800.3A
Authority: CN
Inventors: 王远保; 黄成武
Original assignee: ANHUI NUSCAN BIOLOGICAL ENGINEERING Co Ltd
Current assignee: ANHUI NUSCAN BIOLOGICAL ENGINEERING Co Ltd
Priority date: 2018-02-05
Filing date: 2018-02-05
Publication date: 2018-07-13

Abstract

The invention discloses a kind of Chinese medicine preparations for treating chronic chyluria, including following medicinal material type and weight proportion（By mass percentage）：9 15 grams of Morinda officinalis, raw 10 15 grams of Radix Astragali, 20 30 grams of, Bi Collettiis, 9 15 grams of Cortex Phellodendri, 10 16 grams of radix rehmanniae preparata, 10 15 grams of HERBA CIRSII, 10 15 grams of madder, 30 60 grams of eclipta, 12 30 grams of Radix Codonopsis, 37 grams of RHIZOMA COPTIDIS from Sichuan of China, 12 16 grams of rhizoma atractylodis macrocephalae, 50 60 grams of Poria cocos, 30 40 grams of umbellate pore furgus.For the present invention to treating chronic renal failure chyluria, having reduces albumen and the significant curative effect for eliminating chyluria in urine, without side-effects, convenient to take, cheap.

Description

A kind of Chinese medicine preparation and preparation method thereof for treating chronic chyluria

Technical field

The present invention relates to traditional Chinese medicine correlative technology field, more particularly to a kind of Chinese medicines for treating chronic chyluria Formula and preparation method thereof.

Background technology

Chyluria derives from chronic renal failure, chronic renal failure is chronic as caused by many reasons from broadly Progressive Renal lesion, cause interior metabolism product retention, Water-Electrolyte and acid-base imbalance, endocrine disturbance one Kind syndrome, the state of an illness are complicated and changeable and critical.Western medicine to the pathogenesis of chronic renal failure largely grind at present Study carefully, achieve certain progress, but do not obtain very big breakthrough in terms of clinical treatment, is still continuing to use traditional alimentotheray, kidney Dirty alternative medicine.Traditional Chinese medicine has unique advantage in terms of delaying chronic kidney failure is in progress, protects renal function.Chronic renal failure exists Without corresponding appellation in Chinese medical book, but according to its clinical manifestation belong to " oedema ", " pain in the back ", " cream leaching ", " retention of urine ", The disease categories such as " stranguria ", " obstruction and rejection ", " consumptive disease ", " vomiting ", " dizziness ", " kidney labor ", " kidney wind ", " poison of drowning ".The common waist of patient Knees soreness, dizziness and tinnitus, aversion to cold and cold limbs, night pollakiuria, tired, weak, pale complexion or impotence are yellow, deficiency of food is indigestion and loss of appetite, just Half congealed, pale tongue is fat, and turbid urine is such as fat, or such as dilute nasal mucus, or like water in which rice has been washed.Some urines is thick turbid as wadded a quilt with cotton, when urine not Freely, urethral obstruction；The white middle band pale red of turbid urine having, mostly without pain.Tired or more food fat foods aggravate.Chronic renal failure With the passing of time mostly many Kidney sickness such as chronic glomerulonephritis, chronic pyelonephritis, hypertensive nephropathy, diabetic nephropathy are delayed More it does not form, the interpretation of the cause, onset and process of an illness is complicated, simulataneous insufficiency and excessive, fever and chills cross-reference.According to clinical practice, though we realize chronic renal failure clinic Show different, but its interpretation of the cause, onset and process of an illness develops always related with lung spleen renal dysfunction, qiactivity of triple energizer mistake department, and spleen kidney deficiency is its interpretation of the cause, onset and process of an illness Key, speen-kidney dificiency is throughout.Especially in compensatory stage of renal insufficiency and Decompensated stage, common speen-kidney dificiency card.It can be seen that Speen-kidney dificiency is this void of the chronic renal failure, faces card treatment and focuses on invigorating spleen and kidney.According to recognizing above the interpretation of the cause, onset and process of an illness of speen-kidney dificiency Know, preferable clinical efficacy is obtained using the method for invigorating spleen and kidney.Many Chinese medicine scholars think that spleen is in close relations with kidney, and " kidney is such as Firewood fire, spleen such as ancient cooking vessel kettle ", the transporting of spleen rely the warm transpiration of yang-energy in kidney, precise and tiny can must so transport, the five internal organs must support entirely；And kidney The vital essence hidden also must spleen institute transporting essence of water and grain be supplement nutrition, kidney essense otherwise must be caused deficient, it is biochemical passive.Chinese medicine is removed Consideration deficiency of spleen and stomach is this, it is believed that it is mark to rush down turbid heat-clearing, and spleen has the function of absorption, defeated cloth and spreading aqueous, chronic renal failure Speen-kidney dificiency, spleen transformation of body flaud dysfunction, retention of water-damp in the body generate the pathological products such as water damp phlegm drink；Water damp phlegm is drunk further The transportation capabilities for influencing spleen, cause water is wet not transport, nausea and vomiting, gastral cavilty turgor, giddy, unhappiness, tongue fur occurs in the spleen being blocked by dampness It is white it is greasy, arteries and veins is slow etc. it is a series of it is wet it is turbid in the clinical manifestations that stop.At this time must be turbid for main treatment with dampness elimination, it is wet except completely, drop is inverse to dissipate Preventing or arresting vomiting.With the passing of time damp evil, which is pented up, then changes heat, or taste element heat in vivo, leads to damp and hot stagnation of turbid phlegm in middle-JIAO, influences the elevating function of taste, Clinical common evil of vomitting, abdominal fullness and distention, loss of appetite, the dirty taste of implication, constipation or it is not well, yellowish fur is slightly yellow, wiry and rolling pulse Deng.At this time must use bitter-cold herbs expelling heat product, Qinghua is wet turbid, Xin Xiang open it is scattered, drive away damp evil, integrate for treating chronic kidney function It can failure chyluria.The pathogenic factor of chyluria, is presently considered to be ductus thoracicus obstruction, and regional nodes' pipe Inflammatory Lesions cause lymph Dynamic (dynamical) change, lymph enter urinary tract, and chyluria occurs.Chronic renal failure chyluria is a kind of common disease of urinary system Disease, urine are creamy white, as containing being known as hemotochyluria if more blood, expert introduction cause chyluria because being known as very More, chyluria age of onset was highest with 30~60 years old, and compared with doctor trained in Western medicine, this disease of Chinese traditional treatment has a clear superiority.Therefore, it sends out It is bright a kind of necessary to solve the above problems for treating the Chinese medicine preparation of chronic chyluria.

Invention content

The invention belongs to field of traditional Chinese medicine technology, and in particular to a kind of for treating in chronic renal failure chyluria Medicine composition, it is significant in efficacy, it is without side-effects.The object of the present invention is to provide a kind of tcm products, and the product is to treating chronic kidney hypofunction Chyluria is exhausted, there is the significant curative effect for reducing albumen and elimination chyluria in urine, it is without side-effects, it is convenient to take, it is cheap Advantage.

To achieve the above object, the present invention provides the following technical solutions：A kind of Chinese medicine for treating chronic chyluria is matched Side, including following medicinal material type and weight proportion (by mass percentage)：10-15 grams of 9-15 grams of Morinda officinalis, raw Radix Astragali, Bi Collettii 20-30 grams, 9-15 grams of Cortex Phellodendri, 10-16 grams of radix rehmanniae preparata, 10-15 grams of HERBA CIRSII, 10-15 grams of madder, 30-60 grams of eclipta, Radix Codonopsis 12-30 grams, 3-7 grams of RHIZOMA COPTIDIS from Sichuan of China, 12-16 grams of rhizoma atractylodis macrocephalae, 50-60 grams of Poria cocos, 30-40 grams of umbellate pore furgus.

Further, including following medicinal material type and weight proportion (by mass percentage)：10 grams of Morinda officinalis, Radix Astragali 10 Gram 20 grams of, Bi Collettiis, 10 grams of Cortex Phellodendri, 10 grams of radix rehmanniae preparata, 15 grams of HERBA CIRSII, 15 grams of madder, 30 grams of eclipta, 12 grams of Radix Codonopsis, RHIZOMA COPTIDIS from Sichuan of China 5 grams, 12 grams of rhizoma atractylodis macrocephalae, 50 grams of Poria cocos, 30 grams of umbellate pore furgus.

Further, including following medicinal material type and weight proportion (by mass percentage)：10 grams of Morinda officinalis, Radix Astragali 10 Gram 20 grams of, Bi Collettiis, 15 grams of Cortex Phellodendri, 10 grams of radix rehmanniae preparata, 15 grams of HERBA CIRSII, 15 grams of madder, 40 grams of eclipta, 12 grams of Radix Codonopsis, RHIZOMA COPTIDIS from Sichuan of China 5 grams, 12 grams of rhizoma atractylodis macrocephalae, 60 grams of Poria cocos, 30 grams of umbellate pore furgus.

A kind of preparation method of Chinese medicine preparation that treating chronic chyluria, includes the following steps：Include the following steps：It will Starting material with water impregnates 8-12 hours, and extractor extraction keeps boiling 60 minutes, and repetition is secondary, and liquid is pumped into concentration tank, dense It is 1.05 to be reduced to relative density, cooling, and the ethyl alcohol of 4 times of amounts is added, and the edible alcohol that ethyl alcohol is 95% or more is sufficiently stirred, quiet Refrigeration 12 hours is set, is filtered, after filtrate recycling ethanol, is further continued for being concentrated into thick paste, relative density 1.35, measuring temperature is 55 degrees Celsius, Amberlyst process can be taken to remove impurity, auxiliary material be added, auxiliary material is dextrin, is pelletized by granulator, fluid bed It is 60-80 DEG C dry, then by whole grain, packaging, the detection of product is by detecting whether the content of Astragaloside IV closes come fixed output quota product Lattice.

The present invention selects Morinda officinalis, raw Radix Astragali, Bi Collettiis, Cortex Phellodendri, radix rehmanniae preparata, HERBA CIRSII, madder, eclipta, Radix Codonopsis, river yellow Company, rhizoma atractylodis macrocephalae, Poria cocos, umbellate pore furgus combine according to the ratio as raw material, according to certain technique, are processed into granule.

Chinese medicine thinks：Raw Radix Codonopsis, Radix Astragali are invigorated the spleen and benefited qi in side of the present invention, it is believed that Radix Astragali can change store essential substances microscopic material, ripe Huang Nourishing kidney yin, Morinda officinalis and eclipta mend the solid puckery essence of nourishing liver and kidney, rhizoma atractylodis macrocephalae help spleen the moon, Poria cocos, umbellate pore furgus and ooze spleen with Chinese yam is light Wet, alleviating water retention is turbid, and number medicine phases are matched, and spleen kidney plays nourishing YIN for benefiting the kidney, tonifying spleen dehumidifying reaches treating both manifestation and root cause of disease , Bi Collettiis dampness removing, divides altogether with mending Qinghua is turbid, the coptis, Cortex Phellodendri heat-clearing, HERBA CIRSII, madder removing pattogenic heat from the blood and toxic material from the body, and number medicine phases are matched, and warming kidney and eliminating damp, distinguishing is turbid, and nourshing kidney is strong Spleen, the day after tomorrow must mend, then kidney storing essence, spleen consolidate camera shooting function then kidney are difficult to overflow and let out from point from solid, pure and impure, so that kidney network is passed unimpeded qi and blood, make Kidney must supplement nutrition, and main water store essential substances, dampness removing is dispelled turbid.

Modern pharmacological research finds that Radix Astragali has adjustment effect to humoral immunity, has protective effect to kidney, can mitigate kidney Dirty pathology, it is believed that Radix Astragali has preferable therapeutic effect to chronic nephritis chyluria；Poria cocos has anti-aging, antitumor and put forward The effect of high immunity；Glutinous rehmannia has protection stomach lining, inhibits pulmonary fibrosis, promotes the works such as hematopoiesis, hypoglycemic, anti-inflammatory, antitumor With , Bi Collettiis have antitumor, anti-osteoporosis, it is antimycotic, resist myocardial ischemia, anti-trioxypurine, Adjust-blood lipid, prevention of arterial it is athero- hard The effects that change；Morinda officinalis have enhancing immune, adjustment endocrine, promote hematopoiesis, protection myocardial function, anti-aging, it is antifatigue, The effects that antitumor；Cortex Phellodendri has antibacterial, antiviral, anti-arrhythmia, blood pressure lowering, Antiulcer activity, promotes pancreas point It secretes, inhibit the effects that cellular immunity；HERBA CIRSII has vasoconstriction, shortening clotting time, antibacterial, reduction blood cholesterol etc. Effect；Madder has the effects that antitumor, increasing leukocyte, immunological regulation, protect liver；Eclipta have immunosupress, protect liver, The effects that hemostasis, antioxidant radical；The coptis has the effects that antibacterial, antitoxin, anti-inflammatory, hypoglycemic, antitumor；Rhizoma Atractylodis Macrocephalae has Diuresis, expansion blood vessel, antitumor, antibacterial, promotes the effects that hematopoiesis at hypoglycemic, anticoagulation；Radix Codonopsis has antitumor, anti-lack Oxygen anti-aging, antiulcer, enhances human immunity, Gastrointestinal motility adjustment, gastric acid secretion inhibiting, reduces pepsin activity Effect；Umbellate pore furgus has the effects that diuresis, immunological regulation, antitumor, liver protection, radioresistance, antibacterial.

The effect of Chinese medicine and quality standard in the present invention

Poria cocos

【Nature and flavor】It is sweet, light, flat.

【Channel tropism】The thoughts of returning home, spleen, kidney channel

【Effect】Inducing diuresis for removing edema, excreting dampness, invigorating the spleen, calming heart.It is applicable in oedema, phlegm and retained fluid, splenasthenic diarrhea, palpitaition, insomnia.

(1) this product powder canescence.Irregular particle shape agglomerate and branched agglomerate are colourless, and it is gradually molten to meet chloraldurate liquid Change.Hyphae colorless or light brown, it is elongated, it is slightly bent, there is branch, 3~8 μm of diameter, minority is to 16 μm.

(2) it takes this product powder a small amount of, potassium iodide iodine test solution 1 is added to drip, show peony.

(3) this product powder 1g is taken, add diethyl ether 50ml, is ultrasonically treated 10 minutes, and filtration, filtrate is evaporated, and residue adds methanol 1ml makes dissolving, as test solution.Poria cocos control medicinal material lg separately is taken, is made in the same way of control medicinal material solution.According to《Thin-layer chromatography Method test stone operation sequence》(VI B of annex) is tested, and is drawn each 2 μ 1 of above two solution, is put respectively in same silica G thin layer On plate, with one ethyl acetate of toluene, one formic acid (20：5：0.5) it is solvent, is unfolded, take out, dry, sprays with 2% vanillic aldehyde sulphur One ethyl alcohol (4 of acid solution：1) it is clear to be heated to spot development at 105 DEG C for mixed solution.In test sample chromatography, with comparison medicine Wood color is composed on corresponding position, and the principal spot of same color is shown.

Bi Collettii

【Nature and flavor】It is bitter, flat.

【Channel tropism】Return kidney, stomach

【Effect】Dampness removing removes turbid, wind expelling stagnation removing.It is applicable in cream leaching, gonorrhoea, arthralgia pain due to rheumatism.

(1) this product powder is fallow.Amylum body is numerous, simple grain oval, ellipse, similar round, class triangle or not Regular shape, some one end are jagged, and some is in warty, 10~70 μm of diameter, omphalion slit-like, herringbone shape, dotted, and laminated striation is most Unobvious.The more bunchys of needle-like calcium oxalate crystal, it is 90~210 μm long.Parenchyma cell wall slightly thickens, and pit is apparent.Bordered pit vessel is straight 17~84 μm of diameter, pit is apparent.Cork cell's brown color, polygonal.

(2) this product powder 2g is taken, methanol 50ml is added, is heated to reflux 1 hour, is filtered, filtrate is evaporated, and residue adds water 25ml Make dissolving, washed with ether 25m1, discard ether solution, aqueous adds hydrochloric acid 2ml, is heated to reflux 1.5 hours, lets cool, shaken with ether Extraction 2 times, each 25ml is shaken, merges ether solution, volatilizes, residue adds chloroform 1ml to make dissolving, as test solution.Separately Continuous careless Xue control medicinal material 2g is taken, control medicinal material solution is made in the same way of.It is tested according to thin-layered chromatography (VI B of annex), draws above-mentioned two Kind of each 10 μ l of solution are put respectively on same silica gel g thin-layer plate, with chloroform acetone (9：1) it is solvent, is unfolded, takes Go out, dry, sprays with phosphomolybdic acid test solution, it is clear to be heated to spot development at 105 DEG C.In test sample chromatography, with comparison medicine wood color It composes on corresponding position, shows the spot of same color.

Radix Codonopsis

【Nature and flavor】It is sweet, flat.

【Channel tropism】Return lung, the spleen channel.

【Effect】Hinder disease using spleen-lung Qi deficiency disease, qi-blood deficiency syndrome, gas Tianjin two.

【Differentiate】(1) this product cross section：For cork cell's ordered series of numbers to 10 ordered series of numbers, there is lithocyte in outside, individually or in groups.Bolt Internal layer is narrow.Bast is broad, and outside often occurs crack, and dissipating has faint yellow latex dust group, and is often mutually arranged with screen casing flock-mate.Forming layer Cyclization.Xylem vessel is individually dispersed in or several gatherings, is arranged radially.Parenchyma cell contains synanthrin.

(2) this product powder 1g is taken, methanol 25ml is added, is ultrasonically treated 30 minutes, filtration, filtrate is evaporated, and residue adds water 15m1 Make dissolving, by D101 types large pore resin absorption column (internal diameter 1.5cm, pillar height 10cm), is eluted with water 50ml, discard water Liquid, then eluted with 50% ethyl alcohol 50ml, eluent is collected, is evaporated, residue adds methanol 1ml to make dissolving, as test solution. Ling Qu lobetyolins reference substance adds methanol that solution of every 1ml containing 1mg is made, as a contrast product solution.It is (attached according to thin-layered chromatography Record VI B) experiment, 2~4 μ l of test solution, 2 μ l of reference substance solution are drawn, is put respectively in same high-efficient silica gel G lamellaes On, with n-butanol glacial acetic acid water (7：1：0.5) be solvent, be unfolded, take out, dry, spray with 10% ethanol solution of sulfuric acid, 1.0 DEG C to be heated to spot development clear, sets inspected under daylight and ultraviolet lamp (365nm) respectively.In test sample chromatography, with On the corresponding position of reference substance chromatography, the spot or fluorescence spot of same color are shown.

【Extract】It measures according to the hot dipping under ethanol soluble extractives measuring method item, with 45% ethanol as solvent, must not lack In 55.0%.

Radix Rehmanniae

【Nature and flavor】It is sweet, bitter, cold.

【Channel tropism】The thoughts of returning home, liver and kidney channel

【Effect】Nourishing Yin and promoting production of body fluid, clearing heat and cooling blood.Injury thirst, Heat Diabetes, yin asthenia generating intrinsic heat, hectic fever due to yin labor heat, heat is applicable in enter Ying blood, the deep red polydipsia of tongue, dry constipation of intestines.

Catalpol shines《High performance liquid chromatography test stone operation sequence》It measures.Chromatographic condition and system suitability Using octadecylsilane chemically bonded silica as filler；With 0.1% phosphoric acid solution (1 of acetonitrile：99) it is mobile phase；Detection wavelength is 210nm.Number of theoretical plate is calculated by Catalpol peak should be not less than 5000.The preparation of reference substance solution takes Catalpol reference substance appropriate, accurate It is weighed, add mobile phase be made the solution of every 1ml μ containing l0 g to get.The preparation of test solution takes this product (radix rehmanniae recen) to be cut into about The fritter of 5mm wears into coarse powder after 80 DEG C are dried under reduced pressure 24 hours, takes about 0.8g, accurately weighed, sets in conical flask with cover, Methanol 50m1 is added in precision, and weighed weight, heating and refluxing extraction 1.5 hours is let cool, then weighed weight, is supplied and is subtracted with methanol The weight of mistake, shakes up, and filtration, precision measures filtered fluid 10ml, is concentrated into and closely does, and residue flowing phased soln is transferred to 10ml In measuring bottle, mobile phase is used in combination to be diluted to scale, shaken up, filter, take subsequent filtrate to get.Measuring method is accurate respectively to draw reference substance Solution and each 10 μ l of test solution inject liquid chromatograph, measure to get.Radix rehmanniae recen is calculated by dry product, contains Catalpol (C15H22O10) 0.20% must not be less than.Acteoside shines《High performance liquid chromatography test stone operation sequence》It measures. Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With one 0.1% acetum of acetonitrile (16：84) it is mobile phase；Detection wavelength is 334nm.Number of theoretical plate is calculated by acteoside peak should be not less than 5000.Control The preparation of product solution takes acteoside reference substance appropriate, accurately weighed, adds mobile phase that solution of every 1ml containing 10ttg is made, i.e., .Test solution prepare it is accurate measure subsequent filtrate 20ml under [assay] item Catalpol item, it is closely dry to be recovered under reduced pressure solvent, residual Slag with flowing phased soln, be transferred in 5ml measuring bottles, add mobile phase to scale, shake up, filter, take filtered fluid to get.Measuring method It is accurate respectively to draw reference substance solution and each 20 μ l of test solution, note people's liquid chromatograph, measure to get.Radix rehmanniae recen is by dry Dry product calculate, and must not be less than 0.020% containing acteoside (C29H36O15), be judged to qualification.

Radix Astragali

【Nature and flavor】Sweet, tepor.

【Channel tropism】Return lung, the spleen channel

【Effect】Tonifying spleen bowl spares, rising Yang act, invigorating qi for consolidating superficies, diuresis, promoting pus discharge and tissue regeneration.It is applicable in Spleen-Qi Deficiency, Lung Qi deficiency, the deficiency of vital energy Spontaneous perspiration, deficiency of qi and blood, drying damp and strengthening spleen, expelling wind and clearing away cold, damp retention in middle-jiao, rheumatic Bi syndrome, chill press from both sides wet table disease.

Astragaloside IV is measured according to high performance liquid chromatography (VI D of annex).

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With acetonitrile water (32： 68) it is mobile phase；Evaporative light scattering detector detects.Number of theoretical plate is calculated by Astragaloside IV peak should be not less than 4000.Reference substance The preparation of solution takes Astragaloside IV reference substance appropriate, accurately weighed, add methanol be made solution of every 1m1 containing 0.5mg to get.For The preparation of test sample solution takes powder about 4g in this product, accurately weighed, sets in Soxhlet extractor, adds methanol 40m1, and cold soaking is overnight, then Add methanol appropriate, be heated to reflux 4 hours, extracting solution recycling design is simultaneously concentrated to dryness, and residue adds water 10ml, low-grade fever to make dissolving, is used Water saturated n-butanol shaking extraction 4 times, each 40ml, merges n-butanol liquid, fully washs 2 times with ammonia solution, every time 40ml discards ammoniacal liquor, and n-butanol liquid is evaporated, and residue adds water 5m1 to make dissolving, lets cool, and passes through D101 type large pore resin absorption columns (internal diameter 1.5cm, pillar height 12cm) is eluted with water 50m1, is discarded aqueous, then eluted with 40% ethyl alcohol 30rnl, is discarded and wash De- liquid elutes after with 70% ethyl alcohol 80ml, collects eluent, be evaporated, residue adds methanol to dissolve, and is transferred in 5ml measuring bottles, adds Methanol to scale, shake up to get.Measuring method is accurate respectively to draw 10 μ l of reference substance solution, 20 μ l, 20 μ l notes of test solution Enter liquid chromatograph, measure, with external standard two-point method logarithmic equation calculate to get.This product is calculated by dry product, contains Astragaloside IV (C41H68O14) 0.040% must not be less than.Calycosin glucoside is measured according to high performance liquid chromatography (VI D of annex). Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；Using acetonitrile as mobility A, with 0.2% formic acid solution is Mobile phase B, and the regulation according to the form below carries out gradient elution；Detection wavelength is 260nm.Number of theoretical plate is pressed Calycosin glucoside peak, which calculates, should be not less than 3000.Time (minute) mobile phase A % Mobile phase Bs %0~2020 → 4080 → 6020~304060, the preparation of reference substance solution takes calycosin glucoside reference substance appropriate, accurately weighed, Add methanol be made every 1ml containing 50 μ g solution to get.The preparation of test solution takes this product powder (crossing No. four sieves) about 1g, essence Close weighed is set in round-bottomed flask, and methanol 50ml is added in precision, and weighed weight is heated to reflux 4 hours, lets cool, then weighed weight, The weight that less loss is supplied with methanol, shakes up, filtration, and precision measures subsequent filtrate 25ml, and for recycling design to doing, residue adds methanol molten Solution, be transferred in jml measuring bottles, add methanol to scale, shake up to get.Measuring method is accurate respectively to be drawn reference substance solution and supplies Each 10 μ l of test sample solution, note people's liquid chromatograph, measure to get.This product is calculated by dry product, glucose containing calycosin Glycosides (C22H22O10) must not be less than 0.020%, be judged to qualification.

According to the content of saponins, flavonoids and polysaccharose substance in Milkvetch Root as defined in Chinese Pharmacopoeia, Huang is determined The evaluation index of stilbene chemical quality standard.HPLC-ELS methods measure the content of Astragaloside IV, and it is always more that Phenol-sulphate acid method measures Radix Astragali The content of sugar.Astragaloside IV, total starches content be respectively 1.186 ± 0.024mg/g, 48.800 ± 1.245mg/g be close Lattice.

Morinda officinalis

【Nature and flavor】Pungent, sweet, tepor.

【Channel tropism】Return kidney, Liver Channel.

【Effect】It tonifies the kidney and support yang, dispelling wind and eliminating dampness.Applicable impotence do not lift, uterus cold infertile, frequent urination, rheumatism lumbocrural pain, Kidney deficiency soreness and weakness of waist and knees.

【Differentiate】(1) this product cross section：Phellem layer is ordered series of numbers cell.On the outside of phelloderm lithocyte it is single or it is several in groups, Intermittent arrangement cyclization；Parenchyma cell contains needle-like calcium oxalate crystal beam, tangential to arrange.Bast is broad, and inside parenchyma cell contains oxalic acid Calcium raphides, axial alignment.Forming layer is apparent.Xylem vessel is individually dispersed in or 2~3 gatherings, is arranged radially, directly Diameter is to 105gm；Wood-fibred is more flourishing；Wide 1~3 row cell of wood radiaftive rays；The wood-parenchymatous cell group of accidental non-woodization.Powder is pale purple Color or puce.Lithocyte is faint yellow, and similar round, class be rectangular, rectangle like, strip or irregular shape, some one end points, 21~96um of diameter, for wall thickness to 39 μm, some laminated striations are apparent, and pit and hole ditch are apparent, and some lithocyte shapes are big, and wall is slightly thick.Grass The sour more bunchys of calcium needle are present in parenchyma cell, and needle is grown to 184um.Bordered pit vessel is faint yellow, diameter to 105um, Bordered pit is fine and closely woven.Fibre tracheid spindle shape, bordered pit is larger, pit aperture R-joining shape or intersects herringbone, cross.

(2) this product powder 2.5g is taken, ethyl alcohol 25ml is added, is heated to reflux 1 hour, lets cool, is filtered, filtrate is concentrated into 1ml, As test solution.Morinda officinalis control medicinal material 2.5g separately is taken, is made in the same way of control medicinal material solution.According to《Thin-layered chromatography is examined S.O.P.》(VI B of annex) is tested, and is drawn each 10tA of above two solution, is put respectively in same silica GF254.Thin layer On plate, with one formic acid (8 of toluene ethyl acetate：2：0.1) it is solvent, is unfolded, takes out, dry, set ultraviolet lamp (254nm) Under inspect.In test sample chromatography, on position corresponding with control medicinal material chromatography, the spot of same color is shown.

【Assay】According to《High performance liquid chromatography test stone operation sequence》It measures.

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With one water (3 of methanol： 97) it is mobile phase；Evaporative light scattering detector detects.Number of theoretical plate is calculated by this resistance to sugared peak should be not less than 2000.

The preparation of reference substance solution takes this resistance to sugared reference substance appropriate, accurately weighed, adds mobile phase that every 1ml is made and contains 0.2mg Solution to get.

The preparation of test solution takes this product powder (crossing No. three sieves) 0.5g, accurately weighed, sets in conical flask with cover, essence Close addition mobile phase 50ml, weighed weight heat 30 minutes in boiling water bath, let cool, then weighed weight, supplied and subtracted with mobile phase The weight of mistake, shakes up, place, take supernatant to filter, take subsequent filtrate to get.

Measuring method is accurate respectively to draw reference substance solution 10ul, 30ul, and test solution 10ul injects liquid chromatograph, Measure, with external standard two-point method logarithmic equation calculate to get.

This product is calculated by dry product, and 2.0% must not be less than containing resistance to this sugared (C24H42O21).

Cortex Phellodendri

【Nature and flavor】It is bitter, cold.

【Channel tropism】Return kidney, bladder, large intestine channel.

【Effect】Heat-clearing and damp-drying drug, purging intense heat, except steaming, detoxification sore treatment.It is applicable under humid tropics, puckery pain of heat gonorrhea, damp-heat dysentery, Huang Subcutaneous ulcer, hygropyretic beriberi, impotence card, hectic fever due to yin labor heat, night sweat, seminal emission, sore swollen toxin, eczema itch.

【Differentiate】(1) this product powder foresythia.Fiber foresythia, 16~38 μm of diameter, normal bunchy, peripheral cell is containing grass Sour calcium prismatic crystal forms crystal fiber；Crystal cell wall wood thickens.Lithocyte foresythia, similar round or spindle, diameter 35~ 128 μ rn, some are in branched, the sharp point in branch end, and wall thickness, laminated striation is apparent；The lithocyte for the visible large-scale threadiness having, length are reachable 900μm.Calcium oxalate prismatic crystal is numerous.(2) this product powder 0.2g is taken, 1% acetate methanol solution 40ml is added, in 60 DEG C of supersound process 20 minutes, filtration, filtrate was concentrated into 2ml, as test solution.Cortex Phellodendri control medicinal material 0.1g separately is taken, adds l% acetate methanols 20mI is made in the same way of control medicinal material solution.Hydrochloric acid phellodendrine reference substance is taken again, adds methanol that solution of every lml containing 0.5mg is made, Product solution as a contrast.It is tested according to thin-layered chromatography (VI B of annex), draws above-mentioned each 3~5 μ l of three kinds of solution, put respectively in same On one silica gel g thin-layer plate, with one methanol of chloroform, one water (30：15：4) lower layer's solution is solvent, sets ammonia saturated with vapor Expansion cylinder in, be unfolded, take out, dry, spray with dilute bismuth potassium iodide test solution.In test sample chromatography, with control medicinal material chromatography and On the corresponding position of reference substance chromatography, the spot of same color is shown.

【Assay】Jamaicin shines《High performance liquid chromatography test stone operation sequence》It measures.

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With acetonitrile -0.1% Phosphoric acid solution (50：50) it is mobile phase (to add dodecyl sodium sulfate 0.1g per 100ml)；Detection wavelength is 265nm.Theoretical plate Number is calculated by Berberine hydrochloride peak should be not less than 4000.The preparation of reference substance solution takes Berberine hydrochloride reference substance appropriate, accurate It is weighed, add mobile phase be made solution of every 1ml containing 0.1mg to get.The preparation of test solution takes this product powder (to cross No. three Sieve) about 0.1g, it is accurately weighed, it sets in 100ml measuring bottles, adds mobile phase 80mI, be ultrasonically treated (power 250W, frequency 40kHz) 40 Minute, let cool, be diluted to scale with mobile phase, shake up, filter, take subsequent filtrate to get.Measuring method is accurate respectively to draw control 5 μ l of product solution and 5~20 μ l of test solution inject liquid chromatograph, measure to get.This product is calculated by dry product, containing small Bark of a cork tree alkali must not be less than 3.0% in terms of Berberine hydrochloride (C20H17NO4HCl).Phellodendrine shines High Performance Liquid Chromatographv (VI D of annex) It measures.Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With -0.1% phosphoric acid of acetonitrile Solution (adding dodecyl sodium sulfate 0.2g per 100ml) (36：64) it is mobile phase；Detection wavelength is 284nm.Number of theoretical plate is pressed Hydrochloric acid phellodendrine peak, which calculates, should be not less than 6000.The preparation of reference substance solution takes hydrochloric acid phellodendrine reference substance appropriate, and precision claims It is fixed, add mobile phase be made solution of every 1m1 containing 0.1mg to get.Test solution preparation takes this product powder (crossing No. four sieves) about 0.5g, it is accurately weighed, it sets in conical flask with cover, precision addition mobile phase 25ml, weighed weight, be ultrasonically treated (power 250W, Frequency 40kHz) 30 minutes, it lets cool, then weighed weight, the weight of less loss is supplied with mobile phase, is shaken up, filter, take subsequent filtrate, To obtain the final product.Measuring method is accurate respectively to draw reference substance solution and each 5 μ l of test solution, injects liquid chromatograph, measure to get.

This product is calculated by dry product, containing phellodendrine in terms of hydrochloric acid phellodendrine (C20H23NO4HCl), must not be less than 0.34%.

HERBA CIRSII

【Nature and flavor】It is sweet, bitter, cool.

【Channel tropism】Converge to heart and liver channels.

【Effect】Cooling blood and hemostasis dissipates stasis of blood removing toxicity for eliminating carbuncles.Applicable blood-head goes out blood trouble, heat toxin carbuncle swells.

【Differentiate】(1) this product leaf surface is seen：Upper table chrotoplast polygonal, anticline is straight, and surface horny texture is apparent； The wavy bending of lower epidermis anticline, upper lower epidermis have stomata and nonglandular hair.Stomata infinitive or inequality.Nonglandular hair 3~10 Remaining cell, apical cell are elongated in whiplike, shrinkage distortion.Contain calcium oxalate crystal in mesophyll cell, is in needle tufted more.

(2) this product powder 0.5g is taken, methanol 5ml is added, is ultrasonically treated 30 minutes, filtration, filtrate is evaporated, and residue adds methanol 2ml makes dissolving, as test solution.Field thistle control medicinal material 0.5g separately is taken, is made in the same way of control medicinal material solution.Illiteracy is taken to spend again Glycosides reference substance adds methanol that solution of every 1ml containing 0.5mg is made, as a contrast product solution.According to《Thin-layered chromatography test stone is grasped Make program》(VI B of annex) is tested, and draws each 1ul of above-mentioned three kinds of solution, is put respectively on same polyamide film, with levulinic One butanone of ketone, one ethyl alcohol, one water (1：3：3：13) it is solvent, is unfolded, take out, dry, sprays with alchlor test solution, dry, It sets and is inspected under ultraviolet lamp (365nm).In test sample chromatography, in position corresponding with control medicinal material chromatography and reference substance chromatography On, show the fluorescence spot of same color.

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With methanol one 0.5% Acetum (55：45) it is mobile phase；Detection wavelength is 326nm.Number of theoretical plate is calculated by linarin peak should be not less than 1500.

The preparation of reference substance solution takes linarin reference substance appropriate, accurately weighed, adds methanol that every 1m1 is made containing 0.1mg's Solution to get.

The preparation of test solution takes this product powder (crossing No. four sieves) about 0.1g, accurately weighed, sets in conical flask with cover, Methanol 10ml is added in precision, and weighed weight is ultrasonically treated (power 100W, frequency 40k } Jz) 15 minutes, lets cool, then weighed heavy Amount, the weight of less loss is supplied with methanol, is shaken up, filter, take subsequent filtrate to get.

Measuring method is accurate respectively to draw reference substance solution and each 5 μ l of test solution, injects liquid chromatograph, measures, i.e., .

This product is calculated by dry product, and 0.70% must not be less than containing linarin (C28H32O14).

Madder

【Nature and flavor】It is sweet, cold.

【Channel tropism】Return liver warp.

【Effect】Cooling blood and removing stasis hemostasis, promoting menstruation.It is applicable in out blood trouble, blood stasis, traumatic injury, arthralgia pain due to rheumatism.

【Differentiate】(1) this product root cross section：Cork cell 6~12 arranges, object containing brown.Phelloderm parenchyma cell some contains Reddish brown coloured particles.Phloem cell is smaller.Forming layer is not very apparent.Xylem accounts for the major part of root, all woodization, ray Unobvious.Parenchyma cell beam containing needle-like calcium oxalate crystal.

(2) this product powder 0.2g is taken, add diethyl ether 5ml, shakes several minutes, and filtration, filtrate adds sodium hydroxide test solution 1ml, shakes It shakes, standing makes layering, the aobvious red of water layer；Ether layer is colourless, sets and is observed under ultraviolet lamp (365nm), shows sky blue fluorescence.

(3) this product powder 0.5g is taken, methanol 10ml is added, is ultrasonically treated 30 minutes, filtration, filtrate is concentrated into lml, as Test solution.Madder control medicinal material 0.5g separately is taken, is made in the same way of control medicinal material solution.Rubimaillin reference substance is taken again, is added Solution of every 1ml containing 2.5mg is made in methanol, as a contrast product solution.It tests, draws above-mentioned according to thin-layered chromatography (VI B of annex) Each 5 μ l of three kinds of solution are put respectively on same silica gel g thin-layer plate, with petroleum ether (60~90X：) acetone (4：1) it is solvent, Expansion takes out, dries, set and inspected under ultraviolet lamp (3651rim).In test sample chromatography, with control medicinal material chromatography and right According on the corresponding position of product chromatography, the fluorescence spot of same color is shown.

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With one acetonitrile of methanol 0.2% phosphoric acid solution (25：50：25) it is mobile phase；Detection wavelength is 250nm.Number of theoretical plate is alizarin by rubimaillin, hydroxyl Careless element peak, which calculates, should all be not less than 4000.

The preparation of reference substance solution takes rubimaillin reference substance, purpurin reference substance appropriate, accurately weighed, adds Methanol be respectively prepared every 1ml 0.1mg containing rubimaillin, 40 μ g of hydroxyl rubican solution to get.Test solution Preparation takes this product powder (crossing No. two sieves) about 0.5g, accurately weighed, sets in conical flask with cover, accurate addition methanol 100ml is close Plug, weighed weight are stood overnight, and are ultrasonically treated (power 250W, frequency 40kHz) 30 minutes, are let cool, then weighed weight, are used first Alcohol supplies the weight of less loss, shakes up, and filtration, precision measures subsequent filtrate 50ml, is evaporated, residue adds 25% hydrochloric acid (4 of methanol：1) Mixed solution 20ml dissolvings set heating hydrolysis 30 minutes in water-bath, cool down immediately, triethylamine 3ml is added, mixing is transferred to In 25m1 measuring bottles, add methanol to scale, shake up, filter, take subsequent filtrate to get.Measuring method is accurate respectively to draw reference substance solution 10 μ l and 20 μ l of test solution inject liquid chromatograph, measure to get.

This product is calculated by dry product, must not be less than 0.40% containing rubimaillin (C17H15O4), purpurin (C14H8O5) 0.10% must not be less than.

Eclipta

【Nature and flavor】It is sweet, sour, cold.

【Channel tropism】Return liver and kidney channel.

【Effect】Nourishing liver and kidney, cooling blood and hemostasis.It is applicable in syndrome of yin deficiency of liver and kidney, the card of losing blood of YinXuXueRe.

【Differentiate】(1) this product is taken, after immersion, rubs its cauline leaf with the hands, is shown blackish green.

(2) this product leaf surface is seen：Nonglandular hair is mostly 3 cells, and 260~700 μm long, base cells slightly expand, middle part cell Longer, wall thickening has an apparent verruca, the anxious point of apical cell and short, nearly triangle.Stomata infinitive, accessory cell 3~4 It is a.

(3) this product powder 2g is taken, 70% methanol 20ml is added, is ultrasonically treated 45 minutes, filtration takes filtrate as test sample Solution.Eclipta control medicinal material 2g separately is taken, is made in the same way of control medicinal material solution.It takes non-irrigated lotus glycosides A reference substances appropriate again, adds methanol Solution of every 1ml containing 0.5mg is made, as a contrast product solution.It is tested according to thin-layered chromatography (VI B of annex), it is molten to draw test sample Liquid and each 10 μ l of control medicinal material solution, 5 μ l of reference substance solution are put respectively on same silica gel g thin-layer plate, with dichloromethane-second Acetoacetic ester-methanol-water (30：40：15：3) it is solvent, is unfolded, take out, dry, sprays with vanillin-sulfuric acid test solution, 105 It is clear DEG C to be heated to spot development.In test sample chromatography, on position corresponding with control medicinal material chromatography and reference substance chromatography, The spot of aobvious same color.

【Assay】It is measured according to high performance liquid chromatography (VI D of annex).

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；It is flowing with methanol Phase A, using 0.5% acetum as Mobile phase B, the regulation according to the form below carries out gradient elution；Detection wavelength is 351nm.It is theoretical Plate number is calculated by wedelolactone peak should be not less than 6000.

The preparation of reference substance solution takes wedelolactone reference substance appropriate, accurately weighed, adds methanol that every 1ml is made and contains 10 μ The solution of g to get.

The preparation of test solution takes this product powder (crossing No. three sieves) about 1g, accurately weighed, sets in conical flask with cover, essence 70% ethyl alcohol 50ml of close addition, weighed weight is heated to reflux l hours, lets cool, then weighed weight, and less loss is supplied with 70% ethyl alcohol Weight, shake up, filter, take subsequent filtrate to get.

Measuring method is accurate respectively to draw reference substance solution and each 20 μ l of test solution, injects liquid chromatograph, measures, To obtain the final product.

This product is calculated by dry product, and 0.040% must not be less than containing wedelolactone (C16H12O7).

RHIZOMA COPTIDIS from Sichuan of China

【Nature and flavor】It is bitter, cold.

【Channel tropism】The thoughts of returning home, spleen, stomach, courage, large intestine channel.

【Effect】Heat-clearing and damp-drying drug, purging intense heat and detonicating.It is applicable in damp and hot feeling of fullness, vomiting acid regurgitation, damp-heat dysentery is unconsciousness due to high fever, vexed It is insomnia, blood-head tells nosebleed, carbuncle swells furuncle sore, hot eyes toothache quench one's thirst, control eczema, wet sore, duct outside and suppurate.

【Differentiate】(1) this product cross section：Phellem layer is ordered series of numbers cell, outer to have epidermis, is often fallen off.Cortex is wider, and stone is thin Born of the same parents are single or are dispersed in groups.Pericyclic fiber bunchy or with a small number of lithocytes, equal displaing yellow.Tough type, circular row outside vascular bundle. Xylem yellow, equal woodization, wood-fibred are more flourishing.Marrow is parenchyma cell, no lithocyte.

Refined even marrow has lithocyte.Cloud gross weight layer, pericycle and marrow are without lithocyte.

(2) this product powder 0.25g is taken, methanol 25ml is added, is ultrasonically treated 30 minutes, filtration takes filtrate molten as test sample Liquid.Coptis control medicinal material 0.25g separately is taken, is made in the same way of control medicinal material solution.Berberine hydrochloride reference substance is taken again, adds methanol system At solution of every 1ml containing 0.5mg, product solution as a contrast.According to《Thin-layered chromatography test stone operation sequence》(VI B of annex) Above-mentioned each 1 μ l of three kinds of solution are drawn in experiment, are put respectively on same high-efficient silica gel G lamellaes, different with cyclohexane ethyl acetate Propyl alcohol methanol-water triethylamine (3：3.5：1：1.5：O.5：1) it is solvent, sets the expansion cylinder with strong ammonia solution presaturation 20 minutes Interior expansion, takes out, dries, set and inspected under ultraviolet lamp (365nm).In test sample chromatography, corresponding to control medicinal material chromatography Position on, show 4 or more same colors fluorescence spot；On the corresponding position of reference substance chromatography, the fluorescence of same color is shown Spot.

【Assay】High performance liquid chromatography (VI D of annex) measures.

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；With acetonitrile 0.05mol/l.Potassium dihydrogen phosphate (50：50) (add lauryl sodium sulfate 0.4g in per 100ml, then with phosphorus acid for adjusting pH It is worth to be 4.0) mobile phase；Detection wavelength is 345nm.Number of theoretical plate is calculated by Berberine hydrochloride peak should be not less than 5000.

The preparation of reference substance solution takes Berberine hydrochloride reference substance appropriate, accurately weighed, adds methanol that every 1ml is made and contains 90.5 " solution of g to get.

The preparation of test solution takes this product powder (crossing No. two sieves) about 0.2g, accurately weighed, sets in conical flask with cover, One hydrochloric acid (100 of methanol is added in precision：1) mixed solution 10Oml, close plug, weighed weight are ultrasonically treated (power 250W, frequency Rate 40kHz) 30 minutes, it lets cool, then weighed weight, the weight of less loss is supplied with methanol, is shaken up, filter, precision measures subsequent filtrate 2ml sets 10m.In 1 measuring bottle, add methanol to scale, shake up, filter, take subsequent filtrate to get.

Measuring method is accurate respectively to draw reference substance solution and each 10 μ l of test solution, injects liquid chromatograph, measures, It is control with the peak area of Berberine hydrochloride reference substance, calculates separately containing for jamaicin, epiberberine, coptisine and palmatine Amount is determined with the relative retention time of ingredient chromatographic peak to be measured and Berberine hydrochloride chromatographic peak.

The peak position of epiberberine, coptisine, palmatine, jamaicin, relative retention time should specified value ±；% Within the scope of to get.Relative retention time see the table below：

Ingredient (peak) relative retention time to be measured, jamaicin 0.71, coptisine 0.78, palmatine 0.91

Jamaicin 1.00, this product are calculated by dry product, and in terms of Berberine hydrochloride, (C20H17NO4) containing jamaicin must not lack In 5.5%, jamaicin (C20H17NO4) must not be less than 0.80%, and coptisine (C19H13NO4) must not be less than 1.6%, bar horse Spit of fland (C21H21NO4) must not be less than 1.5%.

Rhizoma atractylodis macrocephalae

【Nature and flavor】It is sweet, bitter, warm.

【Channel tropism】Return stomach, the spleen channel.

【Effect】Strengthening the spleen and replenishing qi, eliminating dampness diuresis, hidroschesis, tocolysis.It is applicable in syndrome of deficiency of spleen qi, spontaneous perspiration due to deficiency of vital energy, spleen deficiency movement of the foetus is not Peace.

【Differentiate】(1) this product powder is fallow.Needle-like calcium oxalate crystal is tiny, 10~32 μm long, is present in parenchyma cell In, a small number of needle diameters are to 4 μm.Fiber yellow, most bunchy, spindle shape, diameter is about to 40 μm, and wall is very thick, woodization, hole ditch Obviously.Lithocyte is faint yellow, similar round, polygonal, rectangle or a small number of spindles, 37~64 μm of diameter.Parenchyma cell contains chrysanthemum Sugar, surface show radial texture.Vessel element is short and small, is reticulate vessel and bordered pit vessel, diameter is to 48 μm.

(2) this product powder 0.5g is taken, n-hexane 2ml is added, is ultrasonically treated 15 minutes, mistake takes filtrate molten as test sample Liquid.Rhizoma Atractylodis Macrocephalae control medicinal material 0.5g separately is taken, is made in the same way of control medicinal material solution.According to《Thin-layered chromatography test stone operation sequence》 (VI B of annex) is tested, and draws above-mentioned freshly prepd each 10 μ l of two kinds of solution, is put respectively on same silica gel g thin-layer plate, with stone Oily ether (60~90 DEG C) ethyl acetate (50：1) it is solvent, is unfolded, take out, dry, sprays with 5% vanillin-sulfuric acid solution, add Heat is clear to spot development.In test sample chromatography, on position corresponding with control medicinal material chromatography, the spot of same color is shown, And a pink principal spot (atractylone) should be shown.

【It checks】Moisture must not cross 15.0% (Ⅸ the first methods of H of annex).

Total ash must not cross 5.0% (Ⅸ K of annex).

Coloration takes this product most coarse powder 1g, accurately weighed, sets in conical flask with cover, adds 55% ethyl alcohol 200ml, use dilute hydrochloric acid PH value is adjusted to 2~3, continuous shaking 1 hour, filtration is drawn filtrate 10ml, set in colorimetric cylinder, according to solution colour inspection technique (Ⅺ the first methods of A of annex) is tested, must not be deeper compared with No. 9 standard color solutions of yellow.【Extract】According to ethanol soluble extractives Hot dipping under measuring method item measures, and with 60% ethanol as solvent, must not be less than 35.0%.

Umbellate pore furgus

【Nature and flavor】It is sweet, light, flat.

【Channel tropism】Return kidney, bladder warp.

【Effect】Inducing diuresis for removing edema, excreting dampness.It is applicable in oedema, difficult urination, diarrhea.

【Differentiate】(1) this product section：Entirety is closely interwoven by mycelia.27~54 μm of outer thickness, mycelia brown, no It is easily separated；Internal hyphae colorless, bending, 2~10 μm of diameter, the visible tabula having have branch or are expanded in nodositas.Mycelia Numerous calcium oxalate prismatic crystals are asked, mostly in the square octahedral bodily form, the bipyramid octahedral bodily form or irregular polyhedrons of rule, diameter 3 ~60 μm, long to 68 μm, several crystallizations sometimes are gathered.

(2) this product powder 1g is taken, methanol 20ml is added, is ultrasonically treated 30 minutes, filtration takes filtrate as test solution. Ergosterol reference substance is taken, adds methanol that solution of every 1ml containing 1mg is made, as a contrast product solution.According to thin-layered chromatography (annex VI B) experiment, test solution 20 μ l, 4 μ l of reference substance solution are drawn, is put respectively on same silica gel g thin-layer plate, with petroleum ether (60~90 DEG C)-ethyl acetate (3：1) it is solvent, is unfolded, take out, dry, sprays with 2% vanillin-sulfuric acid solution, 105 It is clear DEG C to be heated to spot development.In test sample chromatography, on position corresponding with reference substance chromatography, the spot of same color is shown Point.

Chromatographic condition is with system suitability using octadecylsilane chemically bonded silica as filler；It is flowing with methanol Phase；Detection wavelength is 283nm.Number of theoretical plate is calculated by ergosterol peak should be not less than 5000.The preparation of reference substance solution takes wheat Angle sterol reference substance is appropriate, accurately weighed, add methanol be made every 1m1 containing 50 μ g solution to get.The preparation of test solution This product powder (crossing No. four sieves) about 0.5g is taken, it is accurately weighed, it sets in conical flask with cover, accurate addition methanol 10ml is weighed heavy Amount, is ultrasonically treated (power 220W, frequency 50kHz) 1 hour, lets cool, then weighed weight, the weight of less loss is supplied with methanol, is shaken It is even, filtration, take subsequent filtrate to get.Measuring method is accurate respectively to draw reference substance solution and each 20 μ 1 of test solution, injects liquid Chromatography, measure to get.This product is calculated by dry product, and 0.070% must not be less than containing ergosterol (C28H44O).

The technique effect and advantage of the present invention：For the present invention to treating chronic renal failure chyluria, having reduces albumen in urine It is without side-effects with the significant curative effect for eliminating chyluria, it is convenient to take, it is cheap.

Specific implementation mode

The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described reality It is only a part of the embodiment of the present invention to apply example, instead of all the embodiments.Based on the embodiments of the present invention, this field The every other embodiment that those of ordinary skill is obtained without making creative work, belongs to guarantor of the present invention The range of shield.

Case study on implementation one：A kind of Chinese medicine preparation for treating chronic chyluria, including following medicinal material type and weight are matched Than (by mass percentage)：According to normal process, by being processed into granule：9-15 grams of Morinda officinalis, raw 10-15 grams of Radix Astragali, 20-30 grams of Bi Collettii, 9-15 grams of Cortex Phellodendri, 10-16 grams of radix rehmanniae preparata, 10-15 grams of HERBA CIRSII, 10-15 grams of madder, 30-60 grams of eclipta, 12-30 grams of Radix Codonopsis, 3-7 grams of RHIZOMA COPTIDIS from Sichuan of China, 12-16 grams of rhizoma atractylodis macrocephalae, 50-60 grams of Poria cocos, 30-40 grams of umbellate pore furgus.

Chinese medicine material dosage of the present invention largely gropes summary through inventor's progress and show that each raw material all has in range above There is preferable effect, patient one day one is secondary, a month as a treatment course.

Case study on implementation two：A kind of Chinese medicine preparation for treating chronic chyluria, including following medicinal material type and weight are matched Than (by mass percentage)：According to normal process, by being processed into granule：10 grams of 10 grams of Morinda officinalis, Radix Astragali, Bi Collettiis 20 Gram, 10 grams of Cortex Phellodendri, 10 grams of radix rehmanniae preparata, 15 grams of HERBA CIRSII, 15 grams of madder, 30 grams of eclipta, 12 grams of Radix Codonopsis, 5 grams of RHIZOMA COPTIDIS from Sichuan of China, fry it is white 12 grams of art, 50 grams of Poria cocos, 30 grams of umbellate pore furgus, patient one day one is secondary, a month as a treatment course.

Case study on implementation three：A kind of Chinese medicine preparation for treating chronic chyluria, including following medicinal material type and weight are matched Than (by mass percentage)：According to normal process, by being processed into granule：10 grams of 10 grams of Morinda officinalis, Radix Astragali, Bi Collettiis 20 Gram, 15 grams of Cortex Phellodendri, 10 grams of radix rehmanniae preparata, 15 grams of HERBA CIRSII, 15 grams of madder, 40 grams of eclipta, 12 grams of Radix Codonopsis, 5 grams of RHIZOMA COPTIDIS from Sichuan of China, fry it is white 12 grams of art, 60 grams of Poria cocos, 30 grams of umbellate pore furgus, patient one day one is secondary, a month as a treatment course.

Present invention selection is wherein preferably case study on implementation three：10 grams of Morinda officinalis, 10 grams of Radix Astragali, 20 grams of, Bi Collettiis, Cortex Phellodendri 15 Gram, 10 grams of radix rehmanniae preparata, 15 grams of HERBA CIRSII, 15 grams of madder, 40 grams of eclipta, 12 grams of Radix Codonopsis, 5 grams of RHIZOMA COPTIDIS from Sichuan of China, 12 grams of rhizoma atractylodis macrocephalae, Fu 60 grams of Siberian cocklebur, 30 grams of umbellate pore furgus, as raw material, combine, according to certain technique, are processed into granule according to the ratio.The present invention can take The conventional method of Chinese medicine preparation, and used using this formula Chinese medicine as following patient, patient one day one is secondary, and one month is one A course for the treatment of, its clinical value of statistics.

Chinese medicine preparation of the present invention is without side-effects, easy to use, cheap, is counted according to the clinical observation of case more than 300, It was found that Chinese medicine preparation total effective rate 93.7% of the present invention.

Here is the clinical statistics data for counting 296 chyluria patients using Chinese medicine preparation of the present invention：

One, general information：

Statistics treatment patient 206, wherein treatment group 107, man 59, female 48, age 40-80 Sui, average age 61.7 ± 11.96 years old, course of disease 5-55 days, average 23.7 ± 7.13.Control group 99, man 47, female 52, age 42-78 Year, average age 59.6 ± 10.87 years old, course of disease 6-57 days, average 25.1 ± 4.91.Two groups of ages, gender, the course of disease, state of an illness are tight Weight degree compares, and difference is not statistically significant (P ﹥ 0.05), is comparable.

Two, diagnostic evaluation standard：

Chronic renal failure chyluria category deficiency of spleen and stomach type is formulated, 1. urine according to the diagnosis of spleen kidney qi deficiency and damp invasion of lower energizer It is muddy, milky white as swill starch, solve urine when without pain be main disease.2. being lured whenever feeding the diet greasy, albumen is high or when tired It sends out and aggravates.3. urine chyle qualitative test is positive, routine urinalysis has albumen, red blood cell.4. there is the patient of filariid medical history not exist In Chinese medicine preparation therapeutic domain of the present invention.5. when necessary, doing other inspections, sick position can be defined, while checking chronic renal failure Other sick indexs, the course of disease had hypertension, albuminuria, blood urine and cylindruria performance one or more at 3 months or more.Chyluria Exclusion criteria：Urine milky, without grumeleuse person, urine cerise, without grumeleuse person, urine milky accompanies cerise grumeleuse Person urinates chyle negative person, there is odynuria person.Not pressing course of therapy expires 1 month person.

Criterion of therapeutical effect：1. curing:Symptom disappears, and routine urinalysis is normal, and chyle experiment is negative three times.2. improving:Symptom is basic Control, laboratory examination have index improvement.3. not being cured:Symptom and lab index check unchanged.

Three, interpretation of result:

It is analyzed using SPSS15.0 softwares by statistical analysis, as a result to take t to examine between x ± s groups, calculating chart % It indicates, is analyzed using χ 2 and carry out statistical test, there is statistical significance with P ﹤ 0.05 and P ﹤ 0.01.

Chyluria has more disease types, wherein deficiency of spleen and stomach type to be in the great majority, clinical symptoms multilist show turbid urine, it is milky white such as Swill is starched, without based on pain when solving urine.

1. therapy

The method according to the invention, each patient takes 10 grams of one bag of granules daily, is taken with warm water, general 1 month It is as a treatment course.In the Chinese medicine preparation of the present invention of following treatment group, each patient takes 10 grams of one bag of granules daily, It being taken with warm water, the course for the treatment of is adjusted according to the state of an illness, general 1 month or so, if patient also has other diseases to be treated by other diseases, than If hypertension presses hypertension treatment, high fat of blood is treated by high fat of blood.Control group doctor trained in Western medicine antiphlogistic, hemostatic and battalion Medicine supporting treatment is supported, from the aspect of nutrition, drug two, drug is used patient using by specification, as patient also has other diseases It is treated by other diseases, for example hypertension presses hypertension treatment, high fat of blood is treated by high fat of blood.

2. chyluria treatment results count

Chyluria therapeutic effect is analyzed：Treatment group 107, average age 51.56 years old are carried out with Chinese medicine preparation of the present invention Treatment, 99 people of control group, average age 49.17 years old are treated with Western medicine method, and two groups are compared, and in terms of curative effect, there is system Meter learns meaning (P≤0.05).

1 patient's chyluria disease therapeutic effect of table analyzes (treatment group n=107, control group n=99)

3. the preceding two groups of hemorheology in patients for the treatment of change

Before two groups of treatments whole blood viscosity height cut, in cut, undercut, blood viscosity, erythrocyte aggregation index etc. be above it is normal, Show chyluria patient there are high blood coagulation state, after treatment whole blood viscosity height cut, in cut, undercut, blood viscosity, red blood cell it is poly- Aggregate index number is decreased obviously (P ﹤ 0.01), there is statistical significance, control group to decline unobvious (P ﹥ 0.05), two comparison among groups are poor It is different that there is statistical significance (P ﹤ 0.01).

2 two groups of pretherapy and post-treatment blood rheology parameters of patient of table compare (x ± s) (treatment group n=107, control group n= 99)

4. typical case

(1) Zhang, man, 69 years old, retirement had 2 years medical histories of chyluria, does not go for surgical operation, it is big to carry out Anhui traditional Chinese medicine The treatment of the first affiliated hospital is learned, through looking into, patient usually has that the soreness of waist is weak, dizzy, body is partially thin, and urine shows swill shape, has sometimes Blood urine, in fatigue, chyluria can aggravate, and urine is unsmooth, and yellow and greasy fur, thready rapid pulse, urethral orifice have burning heat sensation, laboratory assay, Chyluria chemical examination is positive, urine has sediment, albumen ++, red blood cell ++, leukaemia+, disease belong to deficiency of spleen and stomach, damp invasion of lower energizer, take this Invention Chinese medicine preparation, and report to, untired, not high fat diet, after 7 days, urine shows swill shape and mitigates, and blood urine mitigates, urine Liquid becomes clear, and the soreness of waist is weak, dizzy phenomenon mitigates, and continues to take Chinese medicine preparation of the present invention 26 days, feels that sb.'s illness took a favorable turn, 1 month Afterwards, it has no repeatedly, continuous 3 checks urine chyle negative.

Week certain, female, before 52 years old, 4 years find turbid urine such as water in which rice has been washed shape, the cities Zeng Mou hospital diagnosis be chyluria, It is not cured repeatedly through western medicines in treatment, day is gradually thin.Carry out the treatment of the first affiliated hospital of Anhui University of Chinese Medecine, examines at quarter:The heart uncomfortable in chest Tired, four limbs burnout, the soreness of waist, dizziness, the dry and astringent pain of urethra, red tongue body, tongue are greasy, deficient and rapid pulse, urine examination:PRO(++)、ERY(+)、 LEU (+), chyle experiment is positive, takes the agent of Chinese medicine preparation of the present invention with clearing heat and promoting diuresis, separating clear and excreting turbid, after 10 days, urine is muddy It is turbid slightly to subtract, and restlessness and thirst, the dry and astringent anti-increasing of urethra.This patient is seen, the thin god of shape is tired, and urethra is dry and astringent, and heat, tongue body are thin when brothers' heart Small, deficient and rapid pulse, which is flat deficiency of the kidney virtual loss, has heat wet as continuing to take 7 doses of Chinese medicine preparation medicine of the present invention, all diseases mitigate, and greasy tongue is gradually Move back, renew and take 20 doses, Urethra astringent pain disappears vexed, and feverishness in palms and soles subtracts greatly, after taken medicine 2 months with the Pill of Anemarrhena, Phellodendrom and Rehmannia, all illness, which are known, to be removed, Urine examination is every normal, does not recur again so far.

(3) Zhang, man 77 years old, the year before because of operation on prostate, have chyluria, after because of no specific reasons, there is urine Muddiness is diagnosed as chyluria such as milk shape, is treated in outer court, unsatisfactory curative effect carrys out the first attached doctor of Anhui University of Chinese Medecine Institute treats, and through looking into, B ultrasound does not have manifest symptom, laboratory detection to find：Chyluria chemical examination is positive+, albumen 2+, have in urine big The cotton-shaped grumeleuse of amount group, patient usually have that the soreness of waist is weak, dizzy, and stomach receives difference, and sleep is bad, sometimes abnormal, red tongue body of defecating, Tongue is white, and arteries and veins is thin, and disease belongs to deficiency of spleen and stomach, damp invasion of lower energizer, takes Chinese medicine preparation of the present invention after 7 days, feels that urine muddiness mitigates, but Still there is floccule, still have the soreness of waist weak, dimly red tongue, tongue is thin, and arteries and veins is thin, continues to take Chinese medicine preparation of the present invention 20 days, after 14 It, feeling improves, and checks routine urinalysis, urinates chyle negative, and Urine proteins are negative.

A kind of Chinese medicine preparation for treating chronic chyluria can be made by above 3 groups of embodiments, wherein the 3rd A kind of Chinese medicine preparation effect for treating chronic chyluria made from group embodiment is best, and the present invention is to treating chronic kidney hypofunction Chyluria is exhausted, there is the significant curative effect for reducing albumen and elimination chyluria in urine, it is without side-effects, it is convenient to take, it is cheap.

Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not limited to this hair It is bright, although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, according to So can be with technical scheme described in the above embodiments is modified, or which part technical characteristic is equal It replaces, all within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in this Within the protection domain of invention.

Claims

1. a kind of Chinese medicine preparation for treating chronic chyluria, which is characterized in that including following medicinal material type and weight proportion （By mass percentage）：9-15 grams of Morinda officinalis, raw 10-15 grams of 20-30 grams of, Bi Collettii of Radix Astragali, 9-15 grams of Cortex Phellodendri, 10-16 grams of radix rehmanniae preparata, 10-15 grams of HERBA CIRSII, 10-15 grams of madder, 30-60 grams of eclipta, 12-30 grams of Radix Codonopsis, 3-7 grams of RHIZOMA COPTIDIS from Sichuan of China, rhizoma atractylodis macrocephalae 12-16 Gram, 50-60 grams of Poria cocos, 30-40 grams of umbellate pore furgus.

2. a kind of Chinese medicine preparation for treating chronic chyluria according to claim 1, it is characterised in that：Including following Medicinal material type and weight proportion（By mass percentage）：10 grams of Morinda officinalis, 10 grams of Radix Astragali, 20 grams of, Bi Collettiis, 10 grams of Cortex Phellodendri, radix rehmanniae preparata 10 grams, 15 grams of HERBA CIRSII, 15 grams of madder, 30 grams of eclipta, 12 grams of Radix Codonopsis, 5 grams of RHIZOMA COPTIDIS from Sichuan of China, 12 grams of rhizoma atractylodis macrocephalae, 50 grams of Poria cocos, pig 30 grams of Siberian cocklebur.

3. a kind of Chinese medicine preparation for treating chronic chyluria according to claim 1, which is characterized in that including following Medicinal material type and weight proportion（By mass percentage）：10 grams of Morinda officinalis, 10 grams of Radix Astragali, 20 grams of, Bi Collettiis, 15 grams of Cortex Phellodendri, radix rehmanniae preparata 10 grams, 15 grams of HERBA CIRSII, 15 grams of madder, 40 grams of eclipta, 12 grams of Radix Codonopsis, 5 grams of RHIZOMA COPTIDIS from Sichuan of China, 12 grams of rhizoma atractylodis macrocephalae, 60 grams of Poria cocos, pig 30 grams of Siberian cocklebur.

4. a kind of a kind of preparation side for treating the Chinese medicine preparation of chronic chyluria as described in claim 1-3 any one Method, which is characterized in that include the following steps：Starting material with water to be impregnated 8-12 hours, extractor extraction keeps boiling 60 minutes, Repetition is secondary, and liquid is pumped into concentration tank, and it is 1.05 to be concentrated to relative density, cooling, and the ethyl alcohol of 4 times of amounts, ethyl alcohol 95% is added Above edible alcohol, is sufficiently stirred, and stands refrigeration 12 hours, filters, and after filtrate recycling ethanol, is further continued for being concentrated into thick paste, Relative density is 1.35, and measuring temperature is 55 degrees Celsius, and Amberlyst process can be taken to remove impurity, and auxiliary material is added, and auxiliary material is paste Essence is pelletized by granulator, 60-80 DEG C of fluidized bed drying, then by whole grain, packaging, the detection of product is by detecting Radix Astragali first Whether the content of glycosides carrys out fixed output quota product qualified.