CN115814015A - She medicine twelve-hour cream and preparation method thereof - Google Patents
She medicine twelve-hour cream and preparation method thereof Download PDFInfo
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Abstract
The invention discloses she-shishishichen cream and a preparation method thereof, in particular to the technical field of cream, the she-shichen cream comprises she-shichen, white yam root, LVSHANZHU, tuxiang root, shuizhizang, sodium lauryl sulfate, stearic acid, glycerol, glyceryl monostearate, camellia oil and borneol, camellia oil is selected as an oil phase of the cream, the effect of 'medicine and auxiliary combination' can be exerted, the cream is not only used as an oil phase auxiliary material to promote the forming of the cream, but also the rich components of the camellia oil can play the effects of nourishing skin, promoting the relieving of inflammation and the like.
Description
Technical Field
The invention belongs to the technical field of creams, and particularly relates to she medicine twelve-hour cream and a preparation method thereof.
Background
Twelve hours are dry roots of Clematis florida thunb.var.plena D.Don which is a Ranunculaceae plant, are medicines for medical practice, have warm and pungent properties according to records of the she medical medicine of Fuan she, and have the effects of clearing and activating the channels and collaterals, dredging the orifices, dispelling wind and removing dampness, activating blood and relieving pain, regulating vital energy and relaxing bowels, inducing diuresis and the like, and have bitter and slightly pungent taste according to records of the Chinese herbal medicine; it has warm nature and low toxicity, and can enter liver, spleen and kidney meridians, and has effects of promoting urination, dredging collaterals, regulating qi-flowing, relaxing bowels and removing toxic substance, and can be used for treating rheumarthritis, dysuria, amenorrhea, constipation, abdominal distention, toothache due to pathogenic wind-fire, nebula, insect bite, and jaundice.
Camellia oil (tea oil) is obtained from seeds of Camellia oleifera in Camellia of Theaceae, and has effects of clearing heat, eliminating dampness, killing parasite and removing toxic substance, and has been collected and carried as medicinal oil in Chinese pharmacopoeia (1995 edition). The camellia oil does not contain erucic acid and cholesterol, is edible vegetable oil peculiar to China, and has long history of production and development. According to records of Shanhai Jing, china has over 2300 years of history of cultivating the oil tea. Ben Cao gang mu Shi Yi (supplement to compendium of materia Medica) is characterized in that: "moisten intestines and clear stomach, kill parasites and remove toxicity". The camellia oil is rich in nutrients such as fatty acid, camellin, tea polyphenol, saponin, tannin and the like, and is also rich in antioxidant and squalene with an anti-inflammatory effect. The squalene is helpful for keeping skin soft and healthy, is an antioxidant, can effectively improve skin color and luster, relieve skin diseases such as psoriasis and dermatitis, and has magical curative effect on human immune system. The camellia oil is also rich in vitamin E and trace elements such as calcium, iron, zinc and the like, and the zinc element which is praised as 'flowers of life' by medical scientists and nutriologists has the content 10 times that of the soybean oil. Zinc can protect skin health, and has effects of pachylosis, xerosis cutis, epitheliosis and esophageal keratosis in absence of zinc; the wounds heal slowly and are easy to be infected, and the chilopod and the crab are usually soaked in the tea oil to treat the sore.
The folk prescription mainly prepared from she medicine for twelve hours is found in the process of investigation, excavation and arrangement of she medicine, and is soaked in wine or camellia oil to be externally used for treating arthritis, acute sprain and contusion, lumbocrural pain and the like, so that the effect is remarkable, and the application history is long. However, the she-nationality traditional Chinese medicine twelve-hour primary preparation homemade by people is poor in smearing uniformity, smearing performance and ductility and affects the curative effect and application experience, so that the invention develops proved formulas into the emulsifiable paste according to the application condition of the people, uses a Box-Behnken response surface method, optimizes the formula of the she-nationality traditional Chinese medicine twelve-hour emulsifiable paste by screening the using amounts of stearic acid, glyceryl monostearate and sodium dodecyl sulfate, and simultaneously adopts an acetic acid writhing method, a formaldehyde pain-causing model and a hot plate method to observe the analgesic effect of the she-nationality twelve-hour traditional Chinese medicinal emulsifiable paste, thereby providing a basis for further development and research of the she-nationality traditional Chinese medicine twelve-hour emulsifiable paste.
Disclosure of Invention
The invention aims to provide a she medicine twelve-hour cream and a preparation method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a she's twelve-hour cream comprises twelve-hour, rhizoma Discoreae Nipponicae, LVSHAZHU, radix Inulae Cappae, SHUILIANGCAO, sodium laurylsulfate, stearic acid, glycerol, glyceryl monostearate, oleum Camelliae Japonicae, and Borneolum Syntheticum.
A preparation method of she medicine twelve-hour cream comprises the following steps:
(1) 60g of twelve-hour medicinal materials, namely the twelve-hour drugs, the root of white yam, the LVSHANZHU, the root of tulip, and the five-flavor medicinal materials of the water reservoir are taken, ground into coarse powder and sieved;
(2) Sieving, adding 10 times of ethanol, soaking for 1 hr, and performing ultrasonic treatment for 1 hr; adding 10 times of ethanol into the filter residue, and performing ultrasonic treatment for 1h; adding 5 times of ethanol into the filter residue, and performing ultrasonic treatment for 1h;
(3) Mixing the three extractive solutions, and concentrating under reduced pressure at 60 deg.C to 12ml;
(4) Weighing 0.05g of borneol and 0.03g of ethylparaben, dissolving in 0.5ml of 95% ethanol, adding 1.0g of tea oil, 0.3-0.9g of glyceryl monostearate and 1.5-2.5g of stearic acid after dissolving, and heating in a water bath at 75-80 ℃ to obtain an oil phase for later use;
(5) Weighing 1.0g of glycerol and 0.3-0.9g of sodium dodecyl sulfate, supplementing water to 15g, and heating in a water bath at 75-80 ℃ to serve as a water phase for later use;
(6) Further, under the water bath heating condition of 75-80 ℃, 0.5ml of concentrated extract and water phase are added into the oil phase in turn while stirring, and the she medicine twelve-hour herbal emulsifiable paste is obtained after continuous stirring.
Preferably, the mesh number of the screen when the screen is used in the step (1) is 16 meshes.
Preferably, the concentration of ethanol in the step (2) is 95%.
Preferably, in the step (2), 0.6g of glyceryl monostearate, 2.5g of stearic acid and 0.3g of sodium dodecyl sulfate are added.
Preferably, the temperature of the water bath heating in the above step (4), step (5) and step (6) is 75 ℃.
The invention has the advantages that: the she medicine shishishishichen emulsifiable paste is characterized in that camellia oil is selected as an oil phase of the emulsifiable paste, the effect of 'medicine-assisting integration' can be exerted, the camellia oil is not only used as an oil phase auxiliary material to promote the formation of the emulsifiable paste, but also rich components of the camellia oil can play the effects of nourishing skin, promoting the alleviation of inflammation and the like.
Drawings
FIG. 1 is a contour diagram of A-B.
FIG. 2 is an A-B response surface diagram.
FIG. 3 is a contour plot of A-C.
FIG. 4 is an A-C response surface diagram.
FIG. 5 is a B-C contour plot.
FIG. 6 is a B-C response surface diagram.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the technical scheme in the embodiment of the invention will be clearly and completely described in the following combined with the embodiment of the invention.
Examples
1. Experimental materials
1.1 instruments
Analytical balance model FA1204B (shanghai precision instruments ltd); TCL refrigerator (TCL household appliances limited); centrifuge (semer feishier science); 101-1AB type electrothermal blowing drying oven (Tianjin Sedris laboratories, inc.).
1.2 reagent
Twelve hours, root of white Ningpo Yam, LVSHANZHU, radix Tupistrae chinensis, and Water Tibetan, and the five national herbs are all identified as genuine by teacher of Fujian Chinese medicine university.
Sodium dodecyl sulfate (batch number: 1202021, center for research and development of fine chemical engineering technology in Guangdong province); stearic acid (batch number: CG10070082, beijing Phoenix ceremony Shang Jing Shang Geng responsibility Co., ltd.); glycerol (batch No. 000619, beijing Chemicals Co.); glyceryl monostearate (batch No. C10286479, shanghai Michelin Biotechnology Ltd.); camellia oil (skilful and unrestrained water source grease limited, autonomous county, jiang yao nationality); borneol (batch No. 220115, manufacturer: yunnan Linyuan spices Co., ltd.).
1.3 Experimental animals
100 SPF Kunming mice with half male and half female, the body mass is 18-22 g. The experimental animals were approved by the ethical committee of experimental animals of Beijing university of traditional Chinese medicine and were bred in animal houses of Beijing university of traditional Chinese medicine.
2. Method of producing a composite material
2.1 she medicine twelve-hour cream preparation method
2.1.1 extraction method:
60g of the five medicinal materials are taken, crushed into coarse powder (sieved by a 16-mesh sieve), 10 times of 95% ethanol (600 ml) is added, and the mixture is soaked for 1 hour and subjected to ultrasonic treatment for 1 hour; adding 10 times of 95% ethanol (600 ml) into the filter residue, and performing ultrasonic treatment for 1h; adding 5 times of 95% ethanol (480 ml) into the filter residue, and performing ultrasonic treatment for 1h. The three extracts were combined and concentrated to about 12ml at 60 ℃ under reduced pressure.
2.2.1Box-Behnken response surface method for screening she medicine twelve-hour herbal cream matrix prescription
2.2.1.1 Experimental design
The results of preliminary experiments show that the dosage of stearic acid, glyceryl monostearate and sodium lauryl sulfate is the main factor influencing the cream quality. Therefore, the influence of the amount of glyceryl monostearate (a), the amount of stearic acid (B) and the amount of sodium lauryl sulfate (C) on the comprehensive score (Y) at different levels is explored, and the specific factor levels are set as shown in table 1. In the experiment, Y is used as an evaluation index, each factor is set to be low, medium and high at 3 levels, and the optimal formula of she medicine twelve-hour cream is researched by a Box-Behnken response surface method.
2.2.2.1 cream preparation
Weighing 0.05g of borneol and 0.03g of ethylparaben according to the prescription amount, dissolving in 0.5ml of 95% ethanol, adding 1.0g of tea oil after dissolving, weighing glyceryl monostearate and stearic acid according to the amount in an experimental design table, and heating on a water bath at 75 ℃ to serve as an oil phase for later use; weighing 1.0g of glycerol according to the prescription amount, weighing sodium dodecyl sulfate according to the amount in an experimental design table, supplementing water to 15g, and heating on a water bath at 75 ℃ to be used as an aqueous phase for later use. Further, under the heating condition of water bath at 75 ℃, 0.5ml of concentrated extract and water phase are sequentially added into the oil phase while stirring, and stirring is continued to obtain the cream.
TABLE 1Box-Behnken response surface method optimization she medicine twelve-hour herbal cream substrate prescription experimental design
Note: the she medicine twelve-hour herbal emulsifiable paste has the total mass of 15g.
Evaluation index and method
The research evaluates the quality of the she medicine twelve-hour herbal cream from five aspects of uniformity, spreadability, ductility, centrifugal stability, cold resistance and heat resistance stability of the cream, wherein each aspect accounts for 20 percent, the total score is 100 percent, and the optimal formula is screened according to the final comprehensive score.
The specific evaluation method of each index comprises the following steps: 1) Uniformity: whether the cream is uniform in color and luster, uniform and fine in texture, and whether agglomeration or particles exist; 2) Coating ductility: coating a proper amount of artificial skin (soft silica gel), and observing whether the artificial skin is easy to coat or not in the coating process; 3) Evaluation of centrifugal stability: placing a proper amount of the cream into a centrifugal tube, centrifuging for 15min (R =5 cm) at 4000 r.min < -1 >, and observing whether the layering phenomenon exists or not; 4) Evaluation of Cold resistance stability: sealing appropriate amount of cream, standing at-20 deg.C for 24 hr, and observing whether layering and oil-water separation occur; 5) Evaluation of Heat resistance stability: sealing with appropriate amount of cream, standing at 50 deg.C for 6 hr, and observing whether layering and oil-water separation occur. All the operations are carried out by wrapping the container with tinfoil paper to protect the cream from light.
2.2.4.1 validation experiment
3 samples were prepared in parallel according to the best prescription optimized by the Box-Behnken response surface method, and the values of lightness (L), hue (a), and chroma (b) of 3 batches of cream were measured using an X-rite colorimeter.
2.3 pharmacodynamic Studies
2.3.1 Aspirin cream preparation
The formula process is the same as that of she medicine twelve-hour herbal cream, 0.5ml of concentrated extract is replaced by 0.5ml of 95% ethanol solution of aspirin, and the cream with 0.5% of aspirin addition is obtained.
2.3.1 acetic acid writhing method
24 Kunming mice (with the weight of 20 +/-2 g) are randomly divided into 4 groups of a blank group (blank cream), a positive medicine control group (0.5% aspirin cream), a high-dose group (34% she-nationality Chinese medicinal herb cream (medicinal material/cream)) and a low-dose group (17% she-nationality Chinese medicinal herb cream (medicinal material/cream)), and each group comprises 6 mice. The abdomen was dehaired at 2X 2cm with 8% aqueous sodium sulfide. The next day after depilation, each group was applied to the mouse abdomen at a dose of 0.3g cream/mouse, absorbed by massage, and placed in the mouse cage after the drug was fully absorbed. Each group of mice was dosed for 7 consecutive days, 2 times daily. After the administration for 2 hours on day 7, 0.2ml of 0.6% acetic acid was intraperitoneally injected into each group of mice. The time from acetic acid injection to writhing and the number of writhing within 15min were recorded for each group of mice. And calculating the inhibition rate according to the experimental result.
Inhibition rate = (number of torsion in blank group-number of torsion in administration group)/number of torsion in blank group × 100%
2.3.2 Formaldehyde pain model
24 Kunming mice (weight 20 +/-2 g) were randomly divided into 4 groups of a blank group (blank cream), a positive drug control group (0.5% aspirin cream), a high-dose group (34% she-medicine twelve-hour herbal cream (medicinal material/cream)) and a low-dose group (17% she-medicine twelve-hour herbal cream (medicinal material/cream)), and 6 mice were each group. The abdomen was dehaired at 2X 2cm with 8% aqueous sodium sulfide. The next day after depilation, each group was applied to the mouse abdomen at a dose of 0.3g cream/mouse, absorbed by massage, and placed in the mouse cage after the drug was fully absorbed. Each group of mice was dosed for 7 consecutive days, 2 times daily. 2h after the 7 th day, 2 ul of 1.8mol/l formaldehyde solution is injected subcutaneously into the skin of the left hind paw of each mouse to cause pain. The accumulated time of the mouse licking the feet is taken as an index of pain response, and the accumulated time of the first phase and the accumulated time of the second phase are respectively recorded.
2.3.3 Hot plate method
Placing female mice of Kunming species on a hot plate at (55 +/-0.5) DEG C, recording the time when the animals begin to lick the hind feet, taking the time as a pain threshold value before administration, measuring the pain threshold value for each mouse for 2 times at intervals of 30min, taking 24 mice with the pain threshold average value within 10-30 s, shaving the abdomen, dividing the mice into a blank control group (blank substrate) with an area of about 2cm multiplied by 2cm, and randomly dividing the mice into a high-dose group, a low-dose group and a positive control drug compound dexamethasone acetate cream group. Respectively applying the tested sample on the abdomen depilating area and the four-foot sole. The medicine is applied to each group for 4 times, the first 2 times are applied to abdomen depilation region, the second 2 times are applied to four feet palm, and each time interval is 30min. The pain threshold value is measured 30min, 60 min and 90min after the last administration of each group of mice, and if the pain threshold value exceeds 60s, the dosage is counted as 60 s.
2.4 statistical treatment
Statistical analysis of the data was performed using SPSS16.0 software. The experimental data are all expressed in x +/-s, and the difference is shown as P <0.05 by adopting one-way variance analysis, so that the statistical significance is realized.
3 results
3.1 prescription optimization experiment result of she medicine twelve-hour herbal emulsifiable paste
3.1.1Box-Behnken response surface method preferred prescription experimental design and results thereof Box-Behnken response surface method preferred prescription experimental design results are shown in Table 2.
TABLE 2 test design and scoring result of she medicine twelve-hour herbal cream matrix prescription Box-Behnken response surface method
3.1.2 model fitting the model fitting was performed using Design-Expert software with Y to the independent variables, the fitting model was evaluated using correlation coefficients (r), and by comparing the degree of fit, quadratic polynomial regression equation Y =87.40+1.50 + a +2.75 + b-3.50 + c-0.5000 + a + b-1.0000 a + c +1.50 + b-6.95 + a2+3.05 + b2+3.05 + c2 was obtained, and the analysis of variance is shown in table 3. From this, R2=0.9751, p <0.01, f =30.47, indicating that the model has very significant variability; the simulation losing item P =0.3099 >.
TABLE 3 analysis of variance results
3.1.3 response surface optimization analysis according to the fitting equation, drawing a contour map and a response surface map of the influence of each influence factor on the comprehensive grading by using Design-Expert 12 software, thereby obtaining the influence of the interaction of each influence factor on the grading. See fig. 1-6.
According to regression model analysis, the optimal conditions for preparing the she medicine shishishichen herbal cream are as follows: the amount of glyceryl monostearate 0.642515g, the amount of stearic acid 2.5g and the amount of sodium lauryl sulfate 0.3g, which are combined, is 98.3938. In the optimized experimental design of the Box-Behnken response surface method, the number 10 has the highest comprehensive score of 99 points, and the formula conditions are as follows: 0.6g of glycerin monostearate, 2.5g of stearic acid and 0.3g of sodium dodecyl sulfate. Considering that the fitted optimal conditions and composite score are very close to the formulation conditions and composite score of number 10, and the dosage of number 10 is an integer, the composite score is more advantageous, so the optimal formula of xxx cream number 10 is determined: 0.60g of glycerin monostearate, 2.5g of stearic acid and 0.3g of sodium dodecyl sulfate.
Preparation method of 3.1.4 she medicine twelve-hour herbal cream
Weighing 0.05g of borneol and 0.03g of ethylparaben according to the prescription amount, dissolving in 0.5ml of 95% ethanol, adding 1.0g of tea oil, 0.6g of glyceryl monostearate and 2.5g of stearic acid after dissolving, and heating in a water bath at 75-80 ℃ to obtain an oil phase for later use; weighing 1.0g of glycerol and 0.3g of sodium dodecyl sulfate according to the prescription amount, supplementing water to 15g, and heating on a water bath at 75-80 ℃ to be used as a water phase for later use. Further, under the water bath heating condition of 75-80 ℃, sequentially adding 0.5ml of concentrated extract and water phase into the oil phase while stirring, and continuously stirring to obtain the she-medicine twelve-hour herbal emulsifiable paste.
3.1.5 color difference meter detection result
The she medicine twelve-hour herbal emulsifiable paste of 3 batches prepared by the preferred prescription process under 3.1.4 is semisolid with light yellow, glossy surface, uniform paste and fine texture. The results of the measurement by the colorimeter are shown in Table 4.
TABLE 4 color difference meter detection result of she medicine twelve-hour herbal emulsifiable paste
From the results in table 4, it can be seen that the values of L, a and b of the twelve-hour she medicinal herb cream of the 3 batches measured by the color difference meter have no significant difference, and no statistical significance exists, which indicates that the twelve-hour she medicinal herb cream of the 3 batches has uniform color and good reproducibility among different batches.
3.2 acetic acid writhing method test results
As can be seen from Table 5, the pain inhibition rates of the low-dose group, the high-dose group and the positive drug control group are respectively 21.24%, 44.04% and 52.85%, which indicates that the she medicinal Shichen herbal cream has a certain analgesic effect and shows a good dose-effect relationship, but the effect is weaker than that of the 0.5% aspirin cream.
TABLE 5 influence of she medicine Shichen herbal cream on acetic acid induced writhing response in mice (n =6,x + -s)
Results indicated P <0.05, P <0.01; compared with the positive drug control group, # P <0.05, # P <0.01
3.3 Formaldehyde pain model test results
The result shows that the average time of licking the feet of the mice in the first time phase is 40.17 +/-3.60 s after the mice are caused with the formaldehyde, the average time of licking the feet in the second time phase is 50.17 +/-3.87 s, and the time of licking the feet of the she-she with the twelve-hour herbal emulsifiable paste is obviously reduced (P is less than 0.01). The inhibition rates of the phase I and the phase II of the high-dose group are respectively 25.31 percent and 28.24 percent; the inhibition rates of the phase I and the phase II of the low-dose group are 14.11 percent and 17.62 percent respectively. Both showed good dose-effect relationship compared to the blank group. Pain thresholds were significantly different (P < 0.05) for both low and high dose groups compared to the positive control, and the cumulative addition time for phase I brother was shorter, indicating that XXX cream had a faster onset of action than the 0.5% aspirin cream. See table 6 for details.
TABLE 6 influence of she medicine Shichen herbal cream on acetic acid induced writhing response in mice (n =6,x + -s)
P <0.05, P <0.01; compared with the positive drug control group, # P <0.05, # P <0.01
3.4 Hot plate method test results
The results show that the she-shishishishishishishichen herbal cream high-dose group and the she-shichen herbal cream low-dose group can both finely prolong the pain reaction latency (pain threshold) of mice to heat stimulation, the pain thresholds of the low-dose group and the high-dose group are very different significantly (P is less than 0.01) compared with the pain thresholds of the blank group, the drug effects of the groups are in good dose-effect relationship, and the drug effects of the groups are different significantly (P is less than 0.05) compared with the positive control group. See table 7 for details.
TABLE 7 influence of she medicine twelve-hour herbal cream on hot plate nociceptive response of mice (n =6,x + -s)
P <0.05, P <0.01; compared with the positive drug control group, # P <0.05, # P <0.01
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (6)
1. A she's twelve-hour cream is prepared from twelve-hour, rhizoma Discoreae Nipponicae, LVSHAZHU, radix Inulae Cappae, SHUILIANCHI, sodium laurylsulfate, stearic acid, glycerol, glyceryl monostearate, oleum Camelliae Japonicae, and Borneolum Syntheticum.
2. The method for preparing she's medicine twelve-hour cream according to claim 1, comprising the steps of:
(1) 60g of twelve-hour medicinal materials, namely the twelve-hour drugs, the root of white yam, the LVSHANZHU, the root of tulip, and the five-flavor medicinal materials of the water reservoir are taken, ground into coarse powder and sieved;
(2) Sieving, adding 10 times of ethanol, soaking for 1 hr, and performing ultrasonic treatment for 1 hr; adding 10 times of ethanol into the filter residue, and performing ultrasonic treatment for 1h; adding 5 times of ethanol into the filter residue, and performing ultrasonic treatment for 1h;
(3) Mixing the three extractive solutions, and concentrating under reduced pressure at 60 deg.C to 12ml;
(4) Weighing 0.05g of borneol and 0.03g of ethylparaben, dissolving in 0.5ml of 95% ethanol, adding 1.0g of tea oil, 0.3-0.9g of glyceryl monostearate and 1.5-2.5g of stearic acid after dissolving, and heating in a water bath at 75-80 ℃ to obtain an oil phase for later use;
(5) Weighing 1.0g of glycerol and 0.3-0.9g of sodium dodecyl sulfate, supplementing water to 15g, and heating in a water bath at 75-80 ℃ to serve as a water phase for later use;
(6) Further, under the water bath heating condition of 75-80 ℃, 0.5ml of concentrated extract and water phase are added into the oil phase in turn while stirring, and the she medicine twelve-hour herbal emulsifiable paste is obtained after continuous stirring.
3. The she medicine shishishishichen cream as set forth in claim 2, wherein the number of meshes of the screen used in the step (1) is 16.
4. The method for preparing she-nationality twelve-hour cream according to claim 2, wherein the concentration of ethanol in the step (2) is 95%.
5. The method for preparing she-nationality-medicine twelve-hour cream according to claim 2, wherein the glycerin monostearate in the step (2) is 0.6g, the stearic acid is 2.5g, and the sodium lauryl sulfate is 0.3g.
6. The method for preparing the selaginella doederleinii hieron total biflavone proliposome as claimed in claim 2, wherein the water bath heating temperature in the steps (4), (5) and (6) is 75 ℃.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105708973A (en) * | 2016-04-11 | 2016-06-29 | 赣南医学院 | Tea oil ointment for external use and preparation method of tea oil ointment |
| CN111195309A (en) * | 2020-03-04 | 2020-05-26 | 福建中医药大学 | She medicine twelve-hour compound for treating diabetic foot ulcer |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105708973A (en) * | 2016-04-11 | 2016-06-29 | 赣南医学院 | Tea oil ointment for external use and preparation method of tea oil ointment |
| CN111195309A (en) * | 2020-03-04 | 2020-05-26 | 福建中医药大学 | She medicine twelve-hour compound for treating diabetic foot ulcer |
Non-Patent Citations (1)
| Title |
|---|
| 刘思延: "十二时辰软膏剂制备工艺研究", 中国民族民间医药, vol. 28, no. 22, pages 62 - 66 * |
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