CN115812734B - Application of bactericide in prevention and treatment of citrus canker - Google Patents

Application of bactericide in prevention and treatment of citrus canker Download PDF

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CN115812734B
CN115812734B CN202211670752.XA CN202211670752A CN115812734B CN 115812734 B CN115812734 B CN 115812734B CN 202211670752 A CN202211670752 A CN 202211670752A CN 115812734 B CN115812734 B CN 115812734B
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citrus
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CN115812734A (en
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胡秀筠
蔡德珍
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Jiangxi New Dragon Biotechnology Co ltd
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Abstract

The invention discloses an application of bactericide in preventing and treating citrus canker, which is characterized in that bactericide is prepared into bactericide solution, and the bactericide solution is diluted by adding water and then sprayed to citrus leaves and citrus treetops for use; wherein the bactericide contains the tetramethylol phosphorus cations, and the mass percentage concentration of the tetramethylol phosphorus cations in the bactericide solution is 1-85 wt%; the bactericide has excellent effect in the application of preventing and treating citrus canker; the bactericide containing the tetrakis hydroxymethyl phosphonium cations can be degraded after being applied to preventing and treating citrus canker, has no residue, and can not cause the problem of citrus fruit medicine residue; the bactericide containing the tetrakis hydroxymethyl phosphonium cations belongs to low-toxicity substances and has no teratogenicity and carcinogenicity; the bactericide containing the tetrakis hydroxymethyl phosphonium cations has good compatibility with citrus, and does not cause phytotoxicity to citrus at high concentration.

Description

Application of bactericide in prevention and treatment of citrus canker
Technical Field
The invention belongs to the technical field of bactericides, and particularly relates to an application of a bactericide in preventing and treating citrus canker.
Background
Citrus canker (citrus bacterial CANKER DISEASE) is mainly caused by xanthomonas citri pathogenic species (Xanthomonas citri subsp. Citri, xcc), and mainly infects plants of the genus citrus, the family rutaceae, the genus trifoliate, and the like, and oranges containing orange genes are relatively susceptible to diseases; the canker can infect the leaves, fruits and branches of citrus, and the lesion part forms characteristic lesions similar to a crater; the disease is spread rapidly, difficult to prevent and treat, when leaves and branches are slightly damaged seriously, fallen leaves and dead tips can be caused, the damage to fruits is light, the disease spots are carried on the fruits, the economic value is reduced, the fruits are cracked and fallen fruits when the damage to fruits is heavy, and the serious prevention and control are improper, so that the large economic loss is usually caused.
Agents that are commonly used to control citrus canker include zinc thiazole, copper thiobacteria, copper king, copper calcium sulfate, etc., but have developed greater resistance due to the use of the agents over the years, there is a great need to develop new bactericides for the control of citrus canker.
Disclosure of Invention
In view of the above problems, an object of the present invention is to provide an application of a bactericide in controlling citrus canker, which has an excellent control effect on citrus canker.
The technical scheme adopted by the invention is as follows: an application of bactericide in preventing and treating citrus canker is provided, wherein the bactericide is prepared into bactericide solution, and the bactericide solution is diluted by water and then sprayed to citrus leaves and citrus tree tips for use;
Wherein the bactericide contains the tetramethylol phosphorus cations, and the mass percentage concentration of the tetramethylol phosphorus cations in the bactericide solution is 1-85 wt%.
Preferably, the solvent of the bactericide solution is water or water and an organic solvent; the organic solvent is a water-miscible organic solvent, and the water-miscible organic solvent comprises one or more of methanol, ethanol, glycerol, ethylene glycol, acetonitrile, dimethylformamide and dimethyl sulfoxide.
Preferably, when the bactericide solution is a tetrakis (hydroxymethyl) phosphonium sulfate aqueous solution, the mass percentage concentration of the tetrakis (hydroxymethyl) phosphonium sulfate in the bactericide solution is 1-75%; when the bactericide solution is a tetra-methylol phosphorus chloride aqueous solution, the mass percentage concentration of the tetra-methylol phosphorus chloride in the bactericide solution is 1-80%.
Preferably, the bactericide solution further comprises a first plant growth regulator, wherein the first plant growth regulator is one or more of naphthalene acetic acid, naphthalene acetamide, indole acetic acid, indole butyric acid, diethyl aminoethyl hexanoate, sodium nitrophenolate, gibberellin, brassinolide and brassin.
Preferably, the bactericide solution is mixed with one or more of amino acid, polypeptide, potassium ion, phosphate ion, magnesium ion, calcium ion, zinc ion, iron ion, boric acid, borax, manganese ion, molybdate ion, humic acid and trehalose.
Preferably, the bactericide solution is used in combination with a fertilizer, which is a liquid fertilizer.
Preferably, the liquid fertilizer comprises one or more of a liquid amino acid fertilizer, a liquid humic acid fertilizer, a liquid medium element fertilizer, a liquid trace element fertilizer, a liquid large element fertilizer and an seaweed extract liquid fertilizer.
Preferably, the fungicide solution is used in combination with a pesticide, which is a pesticide, fungicide, second plant growth regulator and nematicide used on citrus. Preferably, the fungicide solution is used in combination with herbicides comprising one or more of citrus orchard herbicides, glyphosate, glufosinate, diquat, paraquat, 2, 4-dichlorophenoxyacetic acid, 2,4 d butyl ester, pelargonic acid and ammonium pelargonate.
Preferably, the herbicide is a citrus orchard herbicide.
The beneficial effects of the technical scheme are that:
(1) The bactericide containing the tetrakis hydroxymethyl phosphonium cations has excellent effect in the application of preventing and treating citrus canker; (2) The bactericide containing the tetrakis hydroxymethyl phosphonium cations has low price and low cost, and is suitable for large-scale use in the citrus industry; (3) The bactericide containing the tetrakis hydroxymethyl phosphonium cations can be degraded after being applied to preventing and treating citrus canker, has no residue, and can not cause the problem of citrus fruit medicine residue; (4) The bactericide containing the tetrakis hydroxymethyl phosphonium cations belongs to low-toxicity substances and has no teratogenicity and carcinogenicity; (5) The bactericide containing the tetrakis hydroxymethyl phosphonium cations has good compatibility to citrus, and does not cause phytotoxicity to citrus under high concentration; (6) The bactericide containing the tetrakis hydroxymethyl phosphonium cations can be fully dissolved in water, can be mixed with pesticide bactericides, herbicides, plant growth regulators, fertilizers and the like commonly used on citrus, is used in combination, is simple and convenient to use, does not need to be used independently, and reduces labor cost.
Detailed Description
The invention is further illustrated by the following specific examples, which should be understood to those skilled in the art that variations and modifications can be made without departing from the principles of the invention, and these should also be considered to be within the scope of the invention.
What is not described in detail in the present specification belongs to the known technology of those skilled in the art.
The invention discloses a bactericide which can be used for preventing and controlling citrus canker and is screened out by using a culturable citrus canker pathogenic bacterium carpet grass yellow monad pathogenic species (Xanthomonas citri subsp. Citri, xcc), and the bactericide is used for preventing and controlling the citrus canker; the bactericide provided by the invention comprises a tetrakis (hydroxymethyl) phosphonium cation with a structure shown in a formula (1),
In the application of the bactericide in preventing and treating citrus canker, the bactericide is prepared into a bactericide solution, and the mass percent concentration of the tetrakis hydroxymethyl phosphonium cations in the bactericide solution is 1-85 wt%; the solvent of the bactericide solution is water or water and an organic solvent, wherein the organic solvent is preferably an organic solvent miscible with water; when the bactericide is applied to preventing and treating citrus canker, the bactericide solution is diluted by water and then sprayed to citrus leaves and citrus treetops.
(1) When the solvent of the bactericide solution is water, the compound containing a tetrakis (hydroxymethyl) phosphonium cation is preferably tetrakis (hydroxymethyl) phosphonium sulfate (THPS), or tetrakis (hydroxymethyl) phosphonium chloride (THPC).
In one embodiment, the biocide solution is an aqueous solution of phosphorus tetramethylol sulfate; the mass percentage concentration of the tetrakis (hydroxymethyl) phosphonium sulfate in the bactericide solution is 1-75wt%; when the bactericide solution is a tetra-methylol phosphorus chloride aqueous solution, the mass percentage concentration of the tetra-methylol phosphorus chloride in the bactericide solution is 1-80 wt%.
The tetrakis (hydroxymethyl) phosphonium sulfate (THPS) and the tetrakis (hydroxymethyl) phosphonium chloride (THPC) containing the tetrakis (hydroxymethyl) phosphonium cations are widely used as biocides in water treatment, oil fields, papermaking and other industries, and can be used as permanent flame retardants for pure cotton and polyester cotton fabrics; the advantages of the tetrakis hydroxymethyl phosphonium cations are: ① The preparation and synthesis process is simple, the cost is low, and the method is suitable for agriculture; ② After being used, the material is quickly degraded into completely harmless substances, so that the material is completely free of residual pollution and safe to the environment; ③ Tetrakis (hydroxymethyl) phosphonium sulfate (THPS) and tetrakis (hydroxymethyl) phosphonium chloride (THPC) are low toxic compounds, and do not have carcinogenicity and teratogenicity; ④ Commercial tetrakis (hydroxymethyl) phosphonium sulfate solution and tetrakis (hydroxymethyl) phosphonium chloride solution with the mass percentage concentration of 70-85 wt% are water solutions, and commercially available tetrakis (hydroxymethyl) phosphonium sulfate solution or tetrakis (hydroxymethyl) phosphonium chloride solution can be conveniently mixed with various plant nutrition fertilizer factors or elements such as amino acid, polypeptide, potassium, phosphorus, magnesium, calcium, zinc, iron, boron, manganese, molybdenum, humic acid, algal polysaccharide and the like.
(2) When the solvent of the bactericide solution is water and an organic solvent, the water-miscible organic solvent includes one or more of methanol, ethanol, glycerol, ethylene glycol, acetonitrile, dimethylformamide (DMF) and Dimethylsulfoxide (DMSO).
In one embodiment, the biocide solution contains, in addition to the tetrakis (hydroxymethyl) phosphonium cations, a plurality of ions, such as one or more of SO4 2-,HSO4 -,SO3 2-,HSO3 -,H2PO4 -,HPO4 2-,PO4 3-,Cl-,Br-,I-,NO3 -,CH3COO-,F- and citrate ions, preferably SO 4 2- and/or Cl -.
In one embodiment, the bactericide is used for preventing and treating citrus canker, and the bactericide solution is mixed with one or more of amino acid, polypeptide, potassium ion, phosphate ion, magnesium ion, calcium ion, zinc ion, iron ion, boric acid, borax, manganese ion, molybdate ion, humic acid and algal polysaccharide; preferably in admixture with zinc ions.
In one embodiment, the bactericide solution further comprises a surfactant and a penetrant when applied to control citrus canker; wherein the surfactant is such as alkyl glycoside, azone or silicone; in the bactericide solution, the mass percentage concentration of the surfactant is 2-20wt%; preferably, the mass percentage concentration of the surfactant in the bactericide solution is 3-10 wt%.
In one embodiment, the bactericide is applied to control citrus canker, and the bactericide solution further comprises a first plant growth regulator, wherein the first plant growth regulator is one or more of naphthalene acetic acid, naphthalene acetamide, indole acetic acid, indole butyric acid, diethyl aminoethyl hexanoate, sodium nitrophenolate, gibberellin, brassinolide and brassin. The first plant growth regulator in the bactericide solution is commonly added in liquid fertilizer and has the function of promoting the growth of plants.
In one embodiment, the bactericide is applied to control citrus canker by combining a bactericide solution with a fertilizer, wherein the fertilizer is a liquid fertilizer comprising one or more of a liquid amino acid fertilizer, a liquid humic acid fertilizer, a liquid medium element fertilizer, a liquid trace element fertilizer, a liquid macroelement fertilizer and a seaweed extract liquid fertilizer.
In one embodiment, the bactericide is applied to control citrus canker by combining the bactericide solution with a pesticide or herbicide, wherein the pesticide is a pesticide, bactericide, second plant growth regulator and nematicide used on citrus; the herbicide comprises one or more of citrus orchard herbicide, glyphosate, glufosinate, diquat, paraquat, 2, 4-dichlorophenoxyacetic acid (2, 4-D), 2, 4-D butyl ester, pelargonic acid and ammonium pelargonate; preferably the pesticide is a bactericide used on citrus; preferably the herbicide is a citrus orchard herbicide.
The pesticide includes mineral oil, imidacloprid, thiamethoxam, thiacloprid, clothianidin, chlorthiamine, acetamiprid, piprolin, fipronil, fenitrothion, dichlorvos, chlorpyrifos, fenitrothion, fenphos, acephate, profenofos, dimethoate, omethoate, triazophos, lambda-cyhalothrin, bifenthrin, permethrin, fenvalerate, fenpropathrin, isoprocarb, carbofuran, carbosulfan, methomyl, iprovalicarb, tebufenozide, chlorpyrifos, chlorfluazuron, methoprene, methoxyfenozide, chlorfenapyr, one or more of flufenoxuron, flonicamid, pymetrozine, metaflumizone, chlorfenapyr, tolfenpyr, indoxacarb, diafenthiuron, formamidine, amitraz, fenpyroximate, hexythiazox, fenazaquin, clofentezine, propargite, trichlorfon, chlorantraniliprole, cyantraniliprole, flubendiamide, spirodiclofen, etoxazole, emamectin, dinotefuran, spirotetramat, bifenazate, sodium rosinate, sulfoxaflor, monosultap, dimehypo, lufenuron, buprofezin, propiconazole, lambda-cyhalothrin, lime, pyrethrin, matrine, nicotine, veratrine, eucalyptol and celadon.
The bactericides include copper hydroxide, copper calcium sulfate, copper complex, copper quinoline, cuprous oxide, copper king, copper subcarbonate, chlorothalonil, four chlorophthalide, pentachloronitrobenzene, sodium disultone, carbendazim, benomyl, thiophanate-methyl, pyrimethanil, cyprodinil, fluazinam, chlorpyrimid, tridemorph, triflumizole, prochloraz manganese salt, imazalil, difenoconazole, propiconazole, flutriafol, flusilazole, hexaconazole, epoxiconazole, fenbuconazole, triticonazole, triadimenol, bitertanol, triadimefon, tebuconazole, diniconazole, myclobutanil, pyribenzoxazole, tetraconazole, ipconazole, imipram, penconazole, metalaxyl, mefenoxam, cymoxanil, dimethomorph, flumetsulam, propargyl, propamocarb, myclobutanil, oxadone, carboxin, fluopicolide, fluopyram, boscalid, fluoamide, thifluzamide, pyraclostrobin, azoxystrobin, kresoxim-methyl, trifloxystrobin, enestroburin, pyraclostrobin, phenyl ether mycofat, picoxystrobin, coumoxystrobin, enestroburin, trifloxystrobin, procymidone, dimethachlor, iprodione, fludioxonil, myclobutanil, tricyclazole, zinc thiazole, metconazole, silthiopham, probenazole, copper thiabendazole, benziothiazolin, bromothalonil, kasugamycin, polyoxin, validamycin, ningnanmycin, shenzinmycin, pyrimidine nucleoside antibiotics, mesogenic fungi, amino oligosaccharins, lentinan, moroxydine hydrochloride, sincalide, pyrim, cyazofamid, ethylicin, zin, zineb, thiram, propineb, lime, dithianon and captan.
The second plant growth regulator comprises one or more of naphthalene acetic acid, sodium nitrophenolate, gibberellin, ethephon, brassin lactone, propionyl brassin lactone, mepiquat chloride, chlormequat chloride, trinexapac-ethyl, paclobutrazol, uniconamide, enadenine, hydroxy enadenine, 2- (acetoxy) benzoic acid, triacontanol, diethyl aminoethyl hexanoate, nucleotides, cyanamide, silafenprox, butyryl hydrazine, S-lurein, chlorpheniramine, prosapocynum, flumetrazine, thidiazuron, pendimethalin and butralin; the second plant growth regulator in this embodiment includes various types of plant growth regulators.
Nematicides include one or more of acephate, fosthiazate, benfophos, chloropicrin and thiophos.
The application will be further illustrated by the following examples.
Example 1
The bactericidal activity of the tetrakis (hydroxymethyl) phosphonium cation (tetrakis (hydroxymethyl) phosphonium) bactericide against the citrus canker pathogen xanthomonas citri (Xanthomonas citri subsp. Citri, xcc) was measured in a 96-well plate method.
1. The xanthomonas citri pathogenic species (Xanthomonas citri subsp. Citri, xcc) in this example was given away from the Gannan university navel orange engineering research center deposit.
2. The medium of xanthomonas carpet citrus pathogenic species (Xanthomonas citri subsp. Citri, xcc) is NA medium. The formulation of NA medium: 10g/L peptone, 3g/L beef extract, 5g/L sodium chloride and distilled water, adjusting the pH to 7.3 by using 1M NaOH solution, sterilizing at 121 ℃ for 30 minutes under high pressure, and cooling to room temperature for standby.
3. The yellow referenced citrus pathogenic species (Xanthomonas citri subsp. Citri, xcc) of this example was cultured using sterilized NA medium, shaking the flask at 28 ℃ for 180rpm to an OD 600 of about 1.0, and diluting 50-fold with sterile water to obtain diluted yellow referenced citrus pathogenic species (Xanthomonas citri subsp. Citri, xcc) bacterial solution for use.
4. The lowest inhibitory concentration MIC values of phosphorus tetramethylol sulfate (THPS) were tested in a row of 96-well plates. For row a, the holes are A1, A2, A3, A4, A5, A6, A7, A8, A9, a10, a11, a12. 200 μl of NA broth sterilized and cooled to room temperature was added, except for A2 and A12.
200 Μl of a medium solution containing 375ppm (weight concentration, 1 ppm=one part per million, calculated as the mass of phosphorus tetramethylolsulfate THPS) of phosphorus Tetramethylolsulfate (THPS) was added to A2. The preparation method comprises diluting commercial aqueous solution of phosphorus tetramethylol sulfate (THPS) with water with mass percentage concentration of 75wt% with NA liquid medium sterilized and cooled to room temperature.
To A3, 200. Mu.l of a medium solution containing 375ppm of phosphorus tetramethylol sulfate (THPS) was further added. At this time, the volume of the liquid in A3 was 400. Mu.l, 200. Mu.l of the solution from A3 was added to A4 and mixed, and 200. Mu.l of the solution from A4 was added to A5 and mixed, and in this manner, two-fold gradient dilution was sequentially performed to A11. Then 200. Mu.l of the solution was taken out of A11 and added to A12. A1 wells were negative controls, NA medium solution only. Negative control means that there is no tetrakis (hydroxymethyl) phosphonium sulfate (THPS) in A1 and bacteria naturally grow in A1.
Finally, 50. Mu.l of the diluted xanthomonas rugosa citrus pathogenic bacterial suspension (Xanthomonas citri subsp. Citri, xcc) in step 3 was added to A1 to A11. A12 is not diluted to form a bacterial solution of xanthomonas rugosa citrus pathogenic strain (Xanthomonas citri subsp. Citri, xcc). A12 is a blank.
After the 96-well plate was incubated in an incubator at 28℃for 48 hours, absorbance values were measured at 595nm for each well using an enzyme-labeled instrument. Holes grown by xanthomonas citri (Xanthomonas citri subsp. Citri, xcc) bacteria will have a large absorbance value, while holes grown by xanthomonas citri (Xanthomonas citri subsp. Citri, xcc) bacteria will not have a small absorbance value or nearly 0. The Minimum Inhibitory Concentration (MIC) value was the concentration at which phosphorus tetramethylol sulfate (THPS) was least capable of inhibiting the growth of xanthomonas citri pathogenic species (Xanthomonas citri subsp. Citri, xcc) of carpet grass. At this concentration, the absorbance is small, or near 0. Whereas the xanthomonas citri (Xanthomonas citri subsp. Citri, xcc) bacteria grow in wells diluted 2 times in the concentration of wells adjacent to the concentration of wells, with a larger absorbance value.
The MIC of phosphorus Tetramethylolsulfate (THPS) for Xanthomonas citri pathogenic bacteria (Xanthomonas citri, xcc) was found to be 37.5ppm.
Example 2
The tetrakis (hydroxymethyl) phosphonium cation (tetrakis (hydroxymethyl) phosphonium) fungicide is matched with different metal ions and amino acid.
Formula 1: 50g of zinc sulfate heptahydrate, 53 g of commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% wt) aqueous solution, 44 g of water, 0.5g of commercial mixed amino acid powder (80% wt) and 10 g of alkyl glycoside APG0810 are uniformly mixed to form a uniform clear solution. The mass percentage of THPS in the prepared solution is 25%.
Formula 2: ferrous sulfate heptahydrate 35 g, commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% wt) aqueous solution 54.4 g, water 50 g, commercial mixed amino acid powder (80% wt) 0.6 g, 12 g alkyl glycoside APG0810, were mixed well to a homogeneous clear solution. The mass percentage of THPS in the prepared solution is 27%.
Formula 3: 45 g of ferrous sulfate heptahydrate, 55.7 g of zinc sulfate heptahydrate, 77 g of commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (mass content 75%wt) aqueous solution, 120 g of water, 1.4 g of commercial mixed amino acid powder (80%wt) and 15 g of alkyl glycoside APG0810 are uniformly mixed to form a uniform clear solution. The mass percentage of THPS in the prepared solution is 18%.
Formula 4: 42 g of anhydrous cupric sulfate, 111 g of commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75%wt) aqueous solution, 80 g of water, 1.4 g of commercial mixed amino acid powder (80%wt) and 16 g of alkyl glycoside APG0810 are uniformly mixed to form a uniform clear solution, and the mass percentage of the THPS in the prepared solution is 33%.
Formula 5: 6.7 g of boric acid, 50g of water, 60.6 g of commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% wt) aqueous solution, 0.6 g of commercial mixed amino acid powder (80% wt) and 11g of alkyl glycoside APG0810 are uniformly mixed to form a uniform clear solution. The mass percentage of THPS in the prepared solution is 35%.
Formula 6: ferrous sulfate heptahydrate 3.3 g, zinc sulfate heptahydrate 17.2 g, boric acid 2.2 g, commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% wt) aqueous solution 100 g, water 80 g, commercial mixed amino acid powder (80% wt) 26.4 g, 15 g alkyl glycoside APG0810 were mixed well to a homogeneous clear solution. The mass percentage of THPS in the prepared solution is 31%.
Example 3
Example of the formulation of THPS aqueous solution and bactericide
10G of zinc thiazole (97.5%) 4g NP-10 (polyoxyethylene nonylphenol ether), 0.2 g of xanthan gum, 4g of sodium lignin sulfonate, 0.2 g of sodium carboxymethyl cellulose, 3g of ethylene glycol, 15 g of commercial aqueous solution of phosphorus (THPS) tetrahydroxymethyl sulfate (75% by weight) and 63.6 g of water to 100 g were added and sand-ground to obtain a suspension of zinc thiazole THPS of 9.75% zinc thiazole and 11.25% THPS.
Example 4
Mixed formulation example of THPS and insecticide
3G of chlorantraniliprole (95%) 3g of NP-10 (nonylphenol polyoxyethylene ether), 0.2 g of xanthan gum, 7g of sodium lignin sulfonate, 0.2 g of sodium carboxymethyl cellulose, 3g of glycerol, 10g of commercial aqueous solution of tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% by weight) and 73.6 g of water are added to 100g, and sand grinding is carried out to obtain a chlorantraniliprole-THPS suspending agent mixture of 2.85% of chlorantraniliprole and 7.5% of THPS.
Example 5
Mixed formulation example of THPS and herbicide
A commercially available aqueous solution of tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% wt) was added 10 g to 70 g of 20% glufosinate solution, and 20g of alkyl glycoside APG0810 was added to 100 g to give a 16% glufosinate, 7.5% THPS in water.
Example 6
Mixed formulation example of THPS and plant growth regulator
0.1 G of brassinolide, 0.1 g of gibberellin, 0.1 g of benzylaminopurine, 0.1 g of naphthylacetic acid, 20 g of commercial tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% by weight) aqueous solution, 16 g of alkyl glycoside APG0810, and 63.6 g of water were added to prepare an aqueous solution containing 15% THPS, 0.1% of brassinolide, 0.1% of gibberellin, 0.1% of benzylaminopurine, and 0.1% of naphthylacetic acid.
Example 7
A commercially available aqueous solution of tetrakis (hydroxymethyl) phosphonium sulfate (THPS) (75% wt) was 60 g, 10 g of the alkyl glycoside APG0810 was added, and 30 g of water was added to prepare 100 g of an aqueous solution containing 45% THPS. Selecting 4-6 years healthy navel orange tree in Ganzhou Dayu county Chi Jiangzhen garden, diluting with 200 times of water, diluting with 400 times of water, diluting with 600 times of water, spraying navel orange tree tips and leaves, and fully spraying the tree tips and leaves. Tree vigor, and leaf phytotoxicity were observed 3 days, 7 days, and 10 days after spraying. As a result, it was found that the 45% THPS aqueous solution of this formulation was diluted 200-fold, 400-fold and 600-fold with water to spray navel orange trees, and that navel oranges grew normally without burning and phytotoxicity-like spots on the leaves, and did not cause navel orange phytotoxicity.
Example 8
A test for controlling citrus canker by using an aqueous solution of phosphorus tetramethylol sulfate (THPS) was conducted in the garden Chi Jiangzhen in Ganza county, 4-5 months.
The area of the test orchard is about 5 mu. The fertility is moderate, the variety is Newhol navel orange, and the tree age is about 6 years. The ulcer disease is serious.
Test agent:
(1) Treatment agent: a commercially available aqueous solution of phosphorus (THPS) tetrakis (hydroxymethyl) sulfate (75% by weight) was added 600 g, 100 g of the alkyl glycoside APG0810 was added, and 300 g of water was added to make up 1000 g of an aqueous solution containing 45% THPS.
(2) Control agent: 20% thiazole Zinc Suspension (SC) (manufactured by Zhejiang New agricultural chemical Co., ltd.) was purchased from the market.
Test set up 4 treatments:
Treatment 1: 400-fold dilution spray of 45% THPS aqueous solution
Treatment 2: 600-fold dilution spray of 45% THPS aqueous solution
Treatment 3: 400-fold dilution spray of 20% thiazole zinc suspending agent (SC)
Treatment 4: clear water spray (blank control)
Each treatment was repeated 3 times for a total of 12 cells, 4 trees per cell. Spraying once on 4 months and 10 days, and spraying once on 5 months and 10 days. And uniformly spraying the whole navel orange tree tip leaves by using an agricultural sprayer. Other bactericides and agents for preventing and treating the ulcer diseases are not used in the test period, and other insect pests and fertilizer water are managed in the same conventional way.
Investigation was performed 7 days, 5 months, 17 days, 14 days, 5 months and 24 days after the administration. All leaves of the young shoots in the current year are investigated, and the leaf numbers of all stages of diseases are counted in a grading manner. Disease leaf grading standard: grade 0, no lesions; grade 1, 1-5 lesions per leaf; grade 3, 6-10 lesions per leaf; grade 5, 11-15 lesions per leaf; grade 7, 16-20 lesions per leaf; grade 9, 21 lesions above each leaf. The test results are shown in Table 1.
As shown by test results, the 45% THPS aqueous solution is diluted 400 times, the 7-day control effect is 80%, and the 14-day control effect is 74.5%; higher than the control agent zinc thiazole treatment; the 400-time dilution of 45% THPS aqueous solution is better than the 600-time dilution.
Table 1 test results
The foregoing is merely one specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions easily contemplated by those skilled in the art within the technical scope of the present invention should be included in the scope of the present invention. The present invention is not an undue matter of the prior art.

Claims (7)

1. The application of the bactericide in preventing and treating citrus canker is characterized in that the bactericide is prepared into a bactericide solution, and the bactericide solution is diluted by water and then sprayed to citrus leaves and citrus treetops for use;
Wherein, when the bactericide solution is a tetrakis (hydroxymethyl) phosphonium sulfate aqueous solution, the mass percentage concentration of the tetrakis (hydroxymethyl) phosphonium sulfate in the bactericide solution is 1% -75%.
2. The use of claim 1, wherein the fungicide solution further comprises a first plant growth regulator, the first plant growth regulator being one or more of naphthalene acetic acid, naphthalene acetamide, indole acetic acid, indole butyric acid, diethyl amine, sodium nitrophenolate, gibberellin, brassinolide, and brassin.
3. The use according to claim 1, wherein the bactericide solution is mixed with one or more of amino acids, polypeptides, potassium ions, phosphate ions, magnesium ions, calcium ions, zinc ions, iron ions, boric acid, borax, manganese ions, molybdate ions, humic acid and trehalose.
4. The use according to claim 1, wherein the bactericide solution is used in combination with a fertilizer, which is a liquid fertilizer.
5. The use according to claim 4, wherein the liquid fertilizer comprises one or more of a liquid amino acid fertilizer, a liquid humic acid fertilizer, a liquid medium element fertilizer, a liquid trace element fertilizer, a liquid macroelement fertilizer and a seaweed extract liquid fertilizer.
6. Use according to claim 1, wherein the fungicide solution is used in combination with pesticides, which are pesticides, fungicides and nematicides used on citrus.
7. The use according to claim 1, wherein the fungicide solution is used in combination with a herbicide comprising one or more of glyphosate, glufosinate, diquat, paraquat, 2, 4-dichlorophenoxyacetic acid, 2,4 d butyl ester, pelargonic acid and ammonium pelargonate.
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000327510A (en) * 1999-03-16 2000-11-28 Sankyo Co Ltd Fungicide for agricultural and horticultural purposes
CN110612990A (en) * 2019-10-30 2019-12-27 黎明职业大学 Special mildew-proof moth-proof composition for financial accounting files
CN114868761A (en) * 2022-04-11 2022-08-09 江西新龙生物科技股份有限公司 Bactericide for preventing and treating citrus greening disease and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000327510A (en) * 1999-03-16 2000-11-28 Sankyo Co Ltd Fungicide for agricultural and horticultural purposes
CN110612990A (en) * 2019-10-30 2019-12-27 黎明职业大学 Special mildew-proof moth-proof composition for financial accounting files
CN114868761A (en) * 2022-04-11 2022-08-09 江西新龙生物科技股份有限公司 Bactericide for preventing and treating citrus greening disease and application thereof

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