CN115786122A - Phosphorus-dissolving cadmium-reducing microbial agent and preparation method and application thereof - Google Patents
Phosphorus-dissolving cadmium-reducing microbial agent and preparation method and application thereof Download PDFInfo
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- CN115786122A CN115786122A CN202211340428.1A CN202211340428A CN115786122A CN 115786122 A CN115786122 A CN 115786122A CN 202211340428 A CN202211340428 A CN 202211340428A CN 115786122 A CN115786122 A CN 115786122A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 claims abstract description 71
- 229910052793 cadmium Inorganic materials 0.000 claims abstract description 70
- 239000002689 soil Substances 0.000 claims abstract description 47
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 35
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 21
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000011574 phosphorus Substances 0.000 claims abstract description 14
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 14
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 13
- 239000000463 material Substances 0.000 claims abstract description 11
- 244000005700 microbiome Species 0.000 claims abstract description 11
- 239000002028 Biomass Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 3
- 238000003756 stirring Methods 0.000 claims abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 239000000725 suspension Substances 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 7
- 239000001963 growth medium Substances 0.000 claims description 7
- 230000009467 reduction Effects 0.000 claims description 7
- 239000000047 product Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 235000015278 beef Nutrition 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 235000009566 rice Nutrition 0.000 claims description 4
- 244000000000 soil microbiome Species 0.000 claims description 4
- 239000006228 supernatant Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000005303 weighing Methods 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 3
- 239000008223 sterile water Substances 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 1
- 240000007594 Oryza sativa Species 0.000 claims 1
- 229910052802 copper Inorganic materials 0.000 claims 1
- 239000010949 copper Substances 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 241000196324 Embryophyta Species 0.000 description 17
- 238000012544 monitoring process Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000007792 addition Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000002253 acid Substances 0.000 description 4
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000209094 Oryza Species 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 2
- 238000010219 correlation analysis Methods 0.000 description 2
- 229910001448 ferrous ion Inorganic materials 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000003381 solubilizing effect Effects 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- NRGIRRZWCDKDMV-UHFFFAOYSA-H cadmium(2+);diphosphate Chemical compound [Cd+2].[Cd+2].[Cd+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O NRGIRRZWCDKDMV-UHFFFAOYSA-H 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- 238000000209 wet digestion Methods 0.000 description 1
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- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a phosphorus-dissolving cadmium-reducing microbial agent and a preparation method and application thereof. The preparation method comprises the following steps: s1, preparation of microbial fermentation liquor: preparing a fermentation culture solution; s2, expanding culture of the microorganisms: adding a phosphorus-dissolving cadmium-reducing microbial agent into a fermentation culture solution; s3, preparation of a microbial agent: after the fermentation culture of the fermentation liquor is carried out for 18 hours, adding biomass materials, mixing, stirring the microbial inoculum every 2 hours for the first 8 hours to ensure that the states of all components in the mixture are uniform, and after 8 hours, naturally stabilizing the mixture for 24 hours to finish the preparation of the phosphate-solubilizing cadmium-reducing microbial inoculum; the preparation method of the microbial inoculum is simple, microorganisms are immobilized by an adsorption mode, the disturbance of the selected materials to the environment is small, the absorption of plants to cadmium is reduced while the effective cadmium of the soil is reduced, and the content of effective phosphorus in the soil is improved.
Description
Technical Field
The invention relates to the technical field of agricultural microbial technology and soil remediation, in particular to a phosphate-solubilizing and cadmium-reducing microbial agent and a preparation method and application thereof.
Background
Cadmium is a silvery-white heavy metal and is a non-essential element for human bodies. Cadmium has high toxicity and long half-life. The grain is closely related to our life, and the production of grain crops cannot leave the soil. The cadmium pollution source of the soil is wide, and the cadmium pollution source can be finally enriched in a human body through a physical chain to cause various hazards to people. Currently, chemical treatment, physical treatment and biological treatment are mainly used for soil cadmium pollution remediation. Chemical treatment can take effect in a short time, but has high environmental disturbance; the physical treatment method requires a large investment in cost, and the biological treatment utilizes some biomass materials or microorganisms to repair polluted sites, and although the time may be long, the biological treatment is an environment-friendly treatment mode and is more and more concerned by people.
In the prior art, most of microorganisms are treated by a single flora, each microorganism has a certain suitable growth environment, and the single flora does not work in certain soil environments and has no universal applicability.
Disclosure of Invention
The invention aims to provide a phosphate-solubilizing cadmium-reducing microbial agent, a preparation method and application thereof, aiming at the defects in the prior art.
The invention relates to a preparation method of a phosphate-solubilizing cadmium-reducing microbial agent, which comprises the following steps:
s1, preparing a microbial fermentation liquid: preparing a fermentation culture solution;
s2, expanding culture of the microorganisms: adding a phosphorus-dissolving cadmium-reducing microbial agent into a fermentation culture solution;
s3, preparation of a microbial agent: after the fermentation culture of the fermentation liquor is carried out for 18 hours, adding biomass materials, mixing, stirring the microbial inoculum every 2 hours for the first 8 hours to ensure that the states of all components in the mixture are uniform, and after 8 hours, naturally stabilizing the mixture for 24 hours to finish the preparation of the phosphate-solubilizing cadmium-reducing microbial inoculum;
the enrichment process of the phosphorus-dissolving cadmium-reducing microbial agent comprises the following steps: preparing bacterial suspension, wherein the preparation method of the aerobic flora soil bacterial suspension is as follows: weighing fresh soil, placing the fresh soil in sterile water for oscillation, standing, and taking supernatant to prepare aerobic flora soil bacteria suspension; and adding the aerobic flora soil bacterium suspension into a culture medium, enriching the microbial agent to obtain an enriched product, and then carrying out passage switching twice to obtain a third generation of the enriched product phosphorus-dissolving and cadmium-reducing microbial agent.
Further, the culture medium is: glucose 10g/L, (NH) 4 ) 2 SO 4 0.5g/L，NaCl 0.3g/L，MgSO 4 0.3g/L，MnSO 4 0.03g/L，FeSO 4 0.03g/L，K 2 SO 4 0.3g/L，Ca 3 (PO 4 ) 2 5g/L，pH 7.0-7.5。
Further, the prepared fermentation culture solution is beef extract: 3g, peptone: 10g, sodium chloride: 5g, pH:7.4-7.6.
Further, the total inoculum size in the fermentation broth was 2%.
Further, the biomass material comprises rice hull powder, and the solid-to-liquid ratio of the biomass material to the mixed fermentation culture solution is 1 g.
A phosphate-dissolving cadmium-reducing microbial agent prepared by the preparation method.
The application of the microbial agent for dissolving phosphorus and reducing cadmium is used for reducing the content of available cadmium in soil and improving the content of available phosphorus in soil.
The phosphorus-dissolving cadmium-reducing microbial agent can reduce the content of available cadmium in soil, thereby achieving the effect of reducing the absorption of crops, improving the content of available phosphorus in the soil and further changing other physical and chemical factors in the soil. The phosphorus-dissolving and cadmium-reducing microbial agent can convert phosphorus which is difficult to be absorbed and utilized by plants in soil into available phosphorus which is easy to be absorbed by the plants, the available phosphorus mainly exists in a phosphate radical form, and cadmium can react with heavy metal to generate precipitate. xM m+ +yPO 4 3- →My(PO 4 )x。
Compared with the prior art, the microbial inoculum has the following beneficial effects: the preparation method of the microbial inoculum is simple, and the microorganisms are immobilized by an adsorption mode, so that the complex preparation process of other microorganism immobilization modes is omitted; the method uses cheap and easily-obtained biomass materials as carriers, utilizes microorganisms to restore the cadmium pollution of the soil, and has low input cost compared with other treatment modes; compared with other chemical treatment modes, the method has the advantages that the disturbance of the selected materials to the environment is small, the absorption of cadmium by plants is reduced while the effective cadmium in the soil is reduced, the content of effective phosphorus in the soil is increased, and the method has popularization and application values.
Drawings
FIG. 1 is a photograph of a nitrogen-fixing microbial agent prepared;
FIG. 2 is a graph showing the change of available cadmium in a soil system (in the graph, standards a, b, c and d represent whether significant differences occur);
FIG. 3 is a soil cadmium morphological analysis chart (Cd (F1) -acid extractable cadmium Cd (F2) -reducible cadmium Cd (F3) -oxidizable cadmium Cd (F4) -residue cadmium, CK is blank group, DP is microbial inoculum group);
FIG. 4 is a diagram showing a part of the physics and chemistry of the system;
FIG. 5 is a monitoring chart of cadmium content in plants and plant growth conditions;
fig. 6 is a graph of the determination of cadmium content in plants (field test, P <0.01, indicates significant differences).
Detailed Description
The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.
Preparation of microbial agent for dissolving phosphorus and reducing cadmium
Firstly, preparing bacterial suspension, wherein the preparation method of the aerobic flora soil bacterial suspension is as follows: weighing 10g of fresh soil, placing the fresh soil in a triangular conical flask filled with 90mL of sterile water, oscillating the soil for 2 at a speed of 150r/min, standing the soil, and taking supernatant to prepare soil bacterial suspension (aerobic); adding 1mL of the aerobic soil suspension into a phosphate solubilizing culture medium (10 g/L of glucose, (NH) 4 ) 2 SO 4 0.5g/L，NaCl 0.3g/L，MgSO 4 0.3g/L，MnSO 4 0.03g/L，FeSO 4 0.03g/L，K 2 SO 4 0.3g/L，Ca 3 (PO 4 ) 2 5g/L, pH 7.0-7.5), enriching the phosphate-solubilizing functional flora to obtain a phosphate-solubilizing enriched product, and then transferring for two times to obtain an enriched product (P) of the third generation. They were then stored in 20% glycerol and stored in a freezer at-80 ℃ for subsequent studies.
Preparing a beef extract peptone culture medium: weighing 3g of beef extract, 10g of peptone and 5g of sodium chloride respectively, and adjusting to 7.4-7.6 by using a sodium hydroxide solution.
Performing overnight amplification culture on the activated strain until OD is in the culture medium system 600 When the concentration reached 1.0-1.2, the culture was stopped. Centrifuging in a freezing separator (with centrifugal condition of 8000r/min,5 min), discarding supernatant, resuspending with sterilized deionized water on a vortex apparatus, and collecting thallus.
And mixing the thallus and the sterilized rice hull powder, and oscillating on a shaking table (the setting condition is 180r/min,24 h) to ensure that the rice hull powder adsorbs and fixes microorganisms, thereby completing the preparation of the microbial agent. Referring to the attached figure 1, the microbial agent for dissolving phosphorus and reducing cadmium is prepared by the technical scheme of the embodiment.
Phosphorus-dissolving cadmium-reducing microbial agent repairing pot experiment
An indoor pot experiment (the diameter of the upper opening of the flowerpot is 23cm, the height is 13cm, and the bottom diameter is 13 cm) is carried out, and each pot is filled with 2kg of aged cadmium-polluted soil. The phosphate-solubilizing microbial agent is added according to the addition amount of 1%. The form of cadmium, available phosphorus and other physical and chemical factors in the system are monitored.
(1) Active cadmium monitoring in soil systems
The effective cadmium in the system is monitored, the higher the effectiveness of the cadmium is, the stronger the activity of the cadmium is, and the cadmium is easy to be absorbed and utilized by plants. The results of the experiment are shown in FIG. 2. Experimental results show that the content of the available cadmium in the soil is remarkably reduced due to the addition of the phosphate solubilizing bacteria agent, and the reduction trend is stable after 25 days.
(2) Monitoring of cadmium morphology in a system
The cadmium form of the system in the soil is further monitored for explaining the reason of the reduction of the bioavailability of the cadmium, and the cadmium form sequentially represents acid extractable cadmium, reducible cadmium, oxidizable cadmium and residue cadmium from left to right and from top to bottom. The bioavailability of cadmium is reduced in turn and the activity is weaker and weaker. The experimental result shows that the acid extractable cadmium content and the reducible cadmium content in the system are obviously reduced, and the oxidizable cadmium content and the residue cadmium content in the system are obviously increased. This explains the decrease of the content of available cadmium in the soil in the system, and the decrease of the bioavailability of cadmium is caused by the transformation of the form of cadmium in the system from an unstable state to a stable state in the system. The results of the experiment are shown in FIG. 3.
(3) Partial physical and chemical monitoring in a system
The data in fig. 4 shows that the pH in the soil was significantly increased compared to the blank treatment group after addition of the microbial inoculum; the organic matter content does not have obvious difference, but is increased on the average value; the ferrous content in the system is obviously increased, the content of ferrous ions can react with the redox environment in the system, and when the content of the ferrous ions is higher, the system tends to the reduction environment. Previous researches show that when the soil tends to an alkaline environment, the activity of cadmium in the soil is reduced, the content of organic matters is increased, the cadmium is combined with heavy metal cadmium, the content of cadmium in an oxidizable state is increased, the environment tends to a reducing environment, and the content of cadmium in a precipitated state such as CdS in a system is higher. In addition, after the phosphorus-solubilizing microbial inoculum is added, the content of available phosphorus in the soil system is obviously increased in the early stage, and a significant difference appears, which indicates that the content of available phosphorus in the soil is increased by the microbial phosphorus-solubilizing microbial inoculum, and explains to a certain extent that the reduction of the cadmium content in the system is caused by cadmium phosphate precipitation generated by the reaction of cadmium and phosphate radical, and explains that the improvement of the growth condition of later plants is related to the improvement of soil nutrients.
(4) Correlation analysis of cadmium morphology and soil physicochemical property
TABLE 1 Stiff correlation analysis of cadmium morphology with soil physicochemical
** p＜0.01， * p＜0.05.
(Positive values represent positive correlations, negative values represent negative correlations, and a symbol represents whether there is a significant difference)
Experimental data show that acid extractable cadmium, reducible cadmium, oxidizable cadmium and residual cadmium are in obvious negative correlation; the biological effectiveness of cadmium is obviously and negatively related to the environment, the biological effectiveness of cadmium is obviously and negatively related to the organic matter content, and the biological effectiveness of cadmium is T and H 2 The index changes such as O and the like are not obvious.
(5) Monitoring cadmium content in plant and plant growth condition
And monitoring the cadmium content in the plant body by adopting a wet digestion method, measuring the height of the plant by adopting a ruler, and measuring the fresh weight of the plant by adopting a balance. The experimental result is shown in fig. 5, and the experimental result shows that the cadmium content in the plants of the phosphorus-solubilizing bacterium agent treatment group is remarkably reduced and the reduction amplitude reaches 36.24% compared with that of the blank treatment group, and the height, fresh weight and the like of the plants are remarkably increased while the cadmium content in the plants is reduced, so that the toxic action of heavy metal cadmium on the plants is relieved by adding the phosphorus-solubilizing bacterium agent.
(6) Application exploration of field experiment
The prepared microbial agent is added into the field cadmium-polluted soil, and lettuce is selected as a research object. And (5) measuring the cadmium content in the plant body. The measurement results are shown in FIG. 6. The data show that the addition of the phosphate-solubilizing microbial inoculant can generate a result basically consistent with that in a laboratory, the cadmium content in a plant body can be obviously reduced, and the reduction degree reaches 32.16%. The microbial agent also has certain application and development prospects.
The above is not relevant and is applicable to the prior art.
While certain specific embodiments of the present invention have been described in detail by way of illustration, it will be understood by those skilled in the art that the foregoing is illustrative only and is not limiting of the scope of the invention, as various modifications or additions may be made to the specific embodiments described and substituted in a similar manner by those skilled in the art without departing from the scope of the invention as defined in the appending claims. It should be understood by those skilled in the art that any modifications, equivalents, improvements and the like made to the above embodiments in accordance with the technical spirit of the present invention are included in the scope of the present invention.
Claims (7)
1. A preparation method of a phosphate-solubilizing cadmium-reducing microbial agent is characterized by comprising the following steps: the method comprises the following steps:
s1, preparing a microbial fermentation liquid: preparing a fermentation culture solution;
s2, expanding culture of the microorganisms: adding a phosphorus-dissolving cadmium-reducing microbial agent into a fermentation culture solution;
s3, preparation of a microbial agent: after the fermentation culture of the fermentation liquor is carried out for 18 hours, adding biomass materials for mixing, stirring the microbial inoculum every 2 hours after the mixing for the first 8 hours to ensure that the state of each component in the mixture is uniform, and after 8 hours, naturally stabilizing the mixture for 24 hours to finish the preparation of the phosphorus-dissolving cadmium-reducing microbial inoculum;
the enrichment process of the phosphate-solubilizing cadmium-reducing microbial agent is as follows: preparing bacterial suspension, wherein the preparation method of the aerobic flora soil bacterial suspension is as follows: weighing fresh soil, placing the fresh soil in sterile water for oscillation, standing, and taking supernatant to prepare aerobic flora soil bacteria suspension; and adding the aerobic flora soil bacterium suspension into a culture medium, enriching the microbial agent to obtain an enriched product, and then carrying out passage switching twice to obtain a third generation of the enriched product phosphorus-dissolving and cadmium-reducing microbial agent.
2. The method of claim 1, wherein: the culture medium is as follows: glucose 10g/L, (NH) 4 ) 2 SO 4 0.5g/L，NaCl 0.3g/L，MgSO 4 0.3g/L，MnSO 4 0.03g/L，FeSO 4 0.03g/L，K 2 SO 4 0.3g/L，Ca 3 (PO 4 ) 2 5g/L，pH 7.0-7.5。
3. The method of claim 1, wherein: the prepared fermentation culture solution is beef extract: 3g, peptone: 10g, sodium chloride: 5g, pH:7.4-7.6.
4. The method of claim 1, wherein: the total inoculum size in the fermentation broth was 2%.
5. The method of claim 1, wherein: the biomass material comprises rice hull powder, and the solid-liquid ratio of the biomass material to the mixed fermentation culture solution is 1 g.
6. A phosphate-solubilizing cadmium-reducing microbial agent prepared by the preparation method of any one of claims 1 to 5.
7. The use of the microbial agent for phosphate solubilization-cadmium reduction according to claim 6, wherein: the cadmium-free copper-based fertilizer is used for reducing the content of available cadmium in soil and improving the content of available phosphorus in the soil.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
| CN106591277A (en) * | 2016-12-07 | 2017-04-26 | 华南农业大学 | Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof |
| CN108192631A (en) * | 2017-12-26 | 2018-06-22 | 湖南恒凯环保科技投资有限公司 | A kind of charcoal base flora composite soil conditioner and its preparation and application |
| CN109336715A (en) * | 2018-10-29 | 2019-02-15 | 裴文韬 | A kind of preparation method of high repairing type passivation cadmium soil remediation bacterial manure |
| CN115029287A (en) * | 2020-09-03 | 2022-09-09 | 中南大学 | Phosphate solubilizing bacterium agent and preparation method and application thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450552A (en) * | 2014-08-17 | 2015-03-25 | 西北大学 | Sulfate reducing bacteria-phosphate solubilizing bacteria and application thereof in combined remediation of cadmium contaminated soil |
| CN106591277A (en) * | 2016-12-07 | 2017-04-26 | 华南农业大学 | Immobilized Bacillus having phosphate dissolving and heavy metal passivation functions and preparation method and application thereof |
| CN108192631A (en) * | 2017-12-26 | 2018-06-22 | 湖南恒凯环保科技投资有限公司 | A kind of charcoal base flora composite soil conditioner and its preparation and application |
| CN109336715A (en) * | 2018-10-29 | 2019-02-15 | 裴文韬 | A kind of preparation method of high repairing type passivation cadmium soil remediation bacterial manure |
| CN115029287A (en) * | 2020-09-03 | 2022-09-09 | 中南大学 | Phosphate solubilizing bacterium agent and preparation method and application thereof |
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