CN115786038B - Fermented spice, preparation method and application thereof - Google Patents
Fermented spice, preparation method and application thereof Download PDFInfo
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- CN115786038B CN115786038B CN202211140443.1A CN202211140443A CN115786038B CN 115786038 B CN115786038 B CN 115786038B CN 202211140443 A CN202211140443 A CN 202211140443A CN 115786038 B CN115786038 B CN 115786038B
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- 235000019504 cigarettes Nutrition 0.000 claims abstract description 98
- 239000002304 perfume Substances 0.000 claims abstract description 59
- 238000000855 fermentation Methods 0.000 claims abstract description 25
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- 239000002002 slurry Substances 0.000 claims abstract description 12
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Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fats And Perfumes (AREA)
Abstract
The invention discloses a preparation method of fermented spice, which comprises the following steps: (1) preparing violet fermentation slurry; (2) preparing a crude extract; (3) Loading and adsorbing the crude extract by using a silica gel chromatographic column, and then sequentially carrying out gradient elution by using ethanol solutions with different concentrations; collecting and combining 50wt% and 90wt% ethanol solution eluates; (4) Concentrating the eluent under reduced pressure to obtain the color-changeable violet perfume, namely the fermented perfume. The invention also discloses the fermented spice and application of the fermented spice in cigarette materials.
Description
Technical Field
The invention belongs to the technical field of essence and spice for cigarettes, and particularly relates to a fermented spice, a preparation method and application thereof.
Background
The cigarette is a kind of hobby that the smoker can smoke the cigarette to meet the physiological and psychological demands. The pH value of the cigarette smoke can reflect the smoke strength, the smoke irritation and the nicotine inhalation quantity felt by a smoker. A great deal of researches show that the pH value of the smoke of the traditional cigarette is mainly concentrated between 5.4 and 6.2.
The tar reduction of cigarettes is an important subject in cigarette production, and the smoke taste is light and the aroma is weakened along with the reduction of tar amount, so that the smoke concentration is increased and the cigarette aroma is improved by means of a feeding and flavoring technology. The natural perfume is one of the main sources of the essence perfume for cigarettes, and has the effects of increasing the smoke concentration, modifying the smoke fragrance and improving the smoke quality.
The natural perfume is mainly derived from plants, and has the characteristics of safety, no toxicity and special aromatic smell. Some pigments in the natural perfume are colored, so that the multi-color functions of bright color and the like of the cigarette material can be realized. The applicant found that some natural flavours containing pigments may change colour with changes in pH. The color natural perfume with the change of the pH value is developed and used in cigarette materials such as cigarette paper, tipping paper, tows and the like, so that the recognition degree of a cigarette product can be increased, a consumer can taste the smoke and the fragrance when smoking the cigarette, the visual enjoyment of the cigarette with the interesting change of the appearance of the cigarette along with the smoke can be obtained, and the attractive force to the consumer is improved.
The invention is proposed for this purpose.
Disclosure of Invention
The invention aims to solve the problems of insufficient functional variety of the existing essence and spice for cigarettes, and the like, provides a fermented spice which is applied to cigarette materials such as cigarette paper, tipping paper, tows, filter tip particles and the like, and can give special flower fragrance and sweet fragrance to cigarettes, simultaneously enable consumers to feel color change of visual appearance of the cigarettes when smoking the cigarettes, and improve interestingness and cigarette identification.
The percentages used in the present invention are mass percentages unless otherwise indicated.
The technical scheme of the invention is as follows:
the first aspect of the invention discloses a method for preparing fermented spice, comprising the following steps:
(1) Grinding, crushing and sterilizing fresh violet petals to obtain violet slurry, fermenting for a period of time by using nocardia-like ST46 microbial inoculum, and sterilizing to obtain violet fermentation slurry;
(2) Extracting the violet fermentation slurry obtained in the step (1) by using 5-10 times of absolute ethyl alcohol through microwaves, separating an extracting solution, and concentrating the obtained filtrate under reduced pressure until the volume is not changed, thus obtaining a crude extracting solution;
(3) Loading and adsorbing the crude extract obtained in the step (2) by using a silica gel chromatographic column, and then sequentially carrying out gradient elution by using 30wt% ethanol, 50wt% ethanol, 70wt% ethanol and 90wt% ethanol solutions with the volume of 3-5 times of the column volume; collecting and combining 50wt% and 90wt% ethanol solution eluates;
(4) Concentrating the eluent under reduced pressure to obtain the color-changeable violet perfume, namely the fermented perfume.
Preferably, the preparation method of the nocardia ST46 microbial inoculum in the step (1) comprises the following steps: every 1000mL of nocardia like ST46 fermentation culture solution subjected to expansion fermentation culture is centrifuged at 3000rpm/min for 10min, the precipitate is washed by sterile water, and finally 20mL of sterile water is uniformly oscillated, and the nocardia like ST46 microbial inoculum is obtained after 10 times dilution during use. Nocardioides ST46 is separated from tobacco soil samples of a tobacco planting base of Kunming tobacco of Yunnan, and is named as Nocardioides sp.ST46 in microbiological classification, and has been preserved in China general microbiological culture Collection center (CGMCC) for 9 months and 25 days in 2020, with a preservation number of CGMCC 4.7686 and an address: the institute of microorganisms of national academy of sciences of China, national center for sciences of No. 1, north Chen West road, chat, of Beijing, city.
Preferably, the nocardia ST 46-like microbial inoculum of the step (1) is fermented as follows: inoculating 10-20 mL nocardia ST46 bacteria agent into each 100g of violet pulp, fermenting for 24-72 h, and sterilizing in a sterilizing pot at 121 ℃ for 30min to obtain violet fermentation pulp.
The invention also discloses the fermented spice prepared by the preparation method.
Preferably, the fermented perfume is a purplish blue clear liquid with a solid content of 60-80 wt%.
In a third aspect the invention discloses the use of the fermented flavour in a smoking material; the cigarette material comprises cigarette paper, tipping paper, filter rod particles and tows.
Preferably, the application method steps are as follows:
(1) In cigarette paper: diluting the color-changeable violet perfume with ethanol for 2-5 times; mixing fungus polysaccharide 5-15 weight portions, propylene glycol 55-65 weight portions and ethanol 25-35 weight portions through shaking for 1-5 hr to obtain coating liquid matrix; uniformly mixing the diluted color-changeable violet perfume with a coating liquid matrix according to the mass ratio of 1:1 to obtain color-changeable violet perfume coating liquid; coating the color-changeable violet spice coating liquid on the cigarette paper by adopting a spray coating, roll shaft embossing or fixed-point spray printing mode to obtain color cigarette paper containing the color-changeable violet spice;
(2) In tipping paper: diluting the color-changeable violet perfume with ethanol for 2-5 times; mixing 5-15 parts of fungal polysaccharide, 55-65 parts of propylene glycol and 25-35 parts of ethanol for 1-5 hours, and adding an ink auxiliary agent accounting for 0.1-1 wt% of the mass of the mixed solution to obtain an ink matrix; uniformly mixing the diluted color-changeable violet perfume with an ink matrix according to the mass ratio of 1:1 to obtain color-changeable violet perfume ink; printing on the tipping paper in a printing mode of gravure printing, flexography, roller shaft embossing or inkjet printing to obtain color tipping paper containing the color-changeable violet perfume; the ink auxiliary agent comprises one or more of a leveling agent, a defoaming agent and a slipping agent;
(3) In the cigarette filter particles: freeze-drying the color-changeable violet spice to obtain color-changeable violet spice freeze-dried powder; uniformly mixing the obtained spice freeze-dried powder and a granule excipient according to the mass ratio of 1:1-1:10, and then placing the mixture into a fluidized bed for spray granulation, wherein an adhesive solution with the concentration of 4-10wt% is sprayed in the granulation process, so as to obtain the color-changeable violet spice granules; adding the obtained color-changeable violet spice particles into a transparent cavity of a multi-element composite filter rod or a filter rod silk bundle gap; the granule excipient comprises one or more of sugar powder, dextrin, lactose, soluble starch, microcrystalline cellulose and mannitol;
(4) In cigarette tows: freeze-drying the color-changeable violet spice to obtain color-changeable violet spice freeze-dried powder; uniformly mixing the spice freeze-dried powder with the fiber master batch according to the mass ratio of 0.1-3 wt% of the fiber master batch of the tows, and forming color tows containing the color-changeable violet spice through a tow production process and a filter tip forming process; or diluting the color-changeable violet spice freeze-dried powder with ethanol, adding the diluted color-changeable violet spice freeze-dried powder into plasticizer triacetin according to the proportion of 1-5wt%, and spraying the plasticizer triacetin into tows through a filter tip forming device to form color filter tips containing the color-changeable violet spice; the fiber master batch is one of acetic acid diacetic acid tablets and polylactic acid.
When the color-changeable violet perfume solution is applied, a certain amount of acid-base regulator is added to realize the color conversion of the color-changeable violet perfume, wherein the acid-base regulator comprises one or more of citric acid, tartaric acid, malic acid, sodium carbonate and sodium bicarbonate.
Conventional natural pigments or other color-changing flavors may also be added to formulate more color-based flavors when color-changing violet flavors are applied.
For better stability of the color-changeable violet perfume, 0.02-1 wt% of stabilizer may be added to enhance the color stability of the color-changeable violet perfume, wherein the stabilizer comprises one or more of ascorbic acid, sodium erythorbate, rutin, malonic acid, etc.
The invention has the following beneficial effects:
1. violet (Latin brand name: matthia incana (L.) R.Br.) is a biennial or perennial herb of the genus Violet of the family Brassicaceae, and has bright color, is a common ornamental plant, and has effects of clearing heat and detoxicating, moistening skin, removing wrinkles and spots, and eliminating halitosis and moistening throat. The existing tobacco essence and spice production technology has no report on development and application of pH color-changing and aroma-adding dual-function violet spice.
2. The invention utilizes the nocardia ST46 of the fungus species to ferment the violet, and then carries out microwave extraction and silica gel column chromatography sectional refining treatment, so that the prepared color-changeable violet spice not only has special fermented flower fragrance, enriches the smell fragrance of cigarettes after being applied to the cigarettes, obviously increases the unique flower fragrance and sweet fragrance characteristics of the smoke, but also has color change along with the smoking of the smoke; without fermentation, or fermentation using other species, has no or poor effect. Research shows that the fermented spice contains rich violet plant pigment, the plant pigment is purple blue when the pH value of the plant pigment is 5-7, the yellow color is gradually deepened when the pH value is more than 7, and the purple red is displayed when the pH value is less than 5; before the fermented spice is applied, pH of the spice solution is biased to acid or alkali by adding an acid-base regulator, then the pattern of the applied cigarette material is purple or yellow, and when the cigarette is made and smoked, the pH of the acid-base regulator is neutralized by the permeation of smoke, so that the pH of the spice solution is changed from purple or yellow to neutral blue-purple, and the color-changing effect is realized. The fermented spice provided by the invention can give special appearance to cigarettes, improve visual interest, improve the moisture feeling of smoke and improve the comprehensive sensory experience and recognition of cigarettes.
3. The preparation method of the fermented spice fully utilizes easily available plant resources, prepares the violet into the color-changeable spice for cigarettes for the first time, enriches the functional types of the spice for cigarettes, has low cost, has no harmful solvent introduction in the preparation process, is green and safe, and is easy to realize industrialization.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the following examples. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention. In addition, the technical features of the embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Example 1: a preparation method of a color-changeable violet perfume comprises the following steps:
(1) Preparation of nocardia ST46 microbial inoculum:
1) Expansion culture of nocardia ST 46: sterilizing the R2A liquid culture medium at 121 ℃ for 30 minutes, picking part of mycelia on the nocardia like ST46 preservation culture medium, inoculating the mycelia into the liquid culture medium, and shake-culturing at 30 ℃ for 72 hours to obtain liquid strains;
2) Preparation of nocardia-like ST46 inoculant: transferring nocardia-like ST46 liquid strain into a fermentation culture medium according to 10% of inoculation amount, shaking and culturing for 7 days at 30 ℃, centrifuging the obtained culture solution at 3000r/min for 10min every 1000mL, washing precipitate with sterile water, shaking the precipitate uniformly with 20mL of sterile water, and diluting 10 times to obtain nocardia-like ST46 microbial inoculum;
(2) And (3) fermenting plants: grinding and crushing fresh violet to obtain violet slurry, sterilizing in a sterilizing pot at 121 ℃ for 30min, inoculating 15mL of nocardia-like ST46 microbial inoculum to each 100g of violet slurry, fermenting in a constant temperature and humidity box at 22 ℃ and 60% for 72h, and sterilizing in a sterilizing pot at 121 ℃ for 30min to obtain violet fermentation slurry;
(3) Microwave-assisted extraction: adding 8 times of absolute ethyl alcohol into the violet fermentation slurry to carry out microwave-assisted extraction for 30min, wherein the microwave power is 500W; filtering the extractive solution, concentrating the filtrate at 45deg.C under reduced pressure until the volume is unchanged to obtain violet crude extract;
(4) Refining by silica gel column chromatography: taking a chromatographic silica gel column with the mass of 4 times of the violet crude extract, washing the column with 95wt% ethanol, and carrying out sample loading and adsorption on the violet crude extract; then sequentially carrying out gradient elution by using 90wt% ethanol, 70wt% ethanol, 50wt% ethanol and 30wt% ethanol solution with the volume of 3 times of the column volume; collecting and combining 90wt% and 50wt% ethanol solution eluents, concentrating under reduced pressure at 45 ℃ to obtain violet clear liquid with a solid content of 75wt%, and adding sodium erythorbate with a liquid mass of 0.02% to mix uniformly to obtain the color-changeable violet spice with obvious floral and sweet fragrance.
Comparative example 1: as in example 1, but without fermentation flavouring step
The violet extract was purified by microwave-assisted extraction and silica gel column chromatography as in step (3) and step (4) of example 1.
Comparative example 2: as in example 1, fresh violet was subjected to plant fermentation using lactobacillus plantarum (strain number: cic c 20265) instead of nocardia-like ST 46; then microwave-assisted extraction and silica gel column chromatography refining are carried out according to the step (3) and the step (4) in the embodiment 1 to obtain the violet extract.
Comparative example 3: as in example 1, fresh violet was subjected to plant fermentation using streptococcus thermophilus (strain number: cic 20372) instead of nocardia-like ST 46; then microwave-assisted extraction and silica gel column chromatography refining are carried out according to the step (3) and the step (4) in the embodiment 1 to obtain the violet extract.
Example 2: the application of the color-changeable violet perfume and violet extract obtained in the example 1, the comparative example 2 and the comparative example 3 in cigarette paper comprises the following steps:
(1) Preparation of color-changeable violet perfume coating liquid
The color-changeable violet perfume and violet extract prepared in example 1, comparative example 2 and comparative example 3 are diluted by 3 times with ethanol respectively, and then sodium bicarbonate saturated solution is added dropwise respectively until the pH value is about 7.5, at this time, the four perfume solutions can be yellow in color; respectively taking 10 parts of fungal polysaccharide, 65 parts of propylene glycol and 25 parts of ethanol, and shaking for 3 hours and uniformly mixing to obtain four coating liquid matrixes; then mixing the yellow color-changeable violet perfume, violet extract and coating liquid matrix according to a ratio of 1: mixing uniformly in a mass ratio of 1 to obtain four kinds of color-changeable violet spice coating liquid.
(2) Preparation and application of color cigarette paper containing color-changeable violet perfume
In the cigarette paper printing process, pattern design is carried out, fixed-point spray printing is adopted, the four color-changeable violet spice coating liquids are respectively printed on cigarette paper to obtain four color cigarette papers containing the color-changeable violet spice, and then the cigarette papers are further rolled into cigarettes to be used as test samples, and the test cigarettes, the control cigarette No. 1, the control cigarette No. 2 and the control cigarette No. 3 are respectively marked; and comparing the cigarette paper which is not coated with any color-changeable violet spice coating liquid with a blank cigarette;
(3) The evaluation results are shown in Table 1.
In terms of appearance, compared with a blank cigarette, the cigarette paper of the test cigarette and the control cigarette has a pattern with a yellow pattern.
In the aspect of smell, the floral fragrance of the control cigarette 1# prepared without any fermentation is not obvious when smelling; the control cigarette 2# prepared by lactobacillus plantarum fermentation has plant spoilage smell when smelling; the flower fragrance prepared by fermentation of streptococcus thermophilus has a stimulating sour taste during sniffing; the color-changeable violet perfume prepared by using nocardia-like ST46 has special fermented floral fragrance and pleasant fragrance, so that the test cigarette smell containing the color-changeable violet perfume also has fresh fermented floral fragrance.
In the aspect of cigarette smoking evaluation, when the cigarettes are ignited for smoking, compared with a blank cigarette, compared with the cigarette 1# of the cigarettes, the flower fragrance in the smoke is increased to some extent, but the miscellaneous gas is obvious, and the smoke coordination is poor. When the control cigarette 2# prepared by lactobacillus plantarum fermentation is smoked, the smoke quantity is increased, the smoke enters the throat to be bitter and astringent, and the aftertaste is poor. When the control cigarette 3# prepared by streptococcus thermophilus fermentation is smoked, the flower fragrance of the smoke is increased, the irritation is obviously increased, the throat dryness is improved, and the smoke coordination is poor. When the test cigarette prepared by fermenting Nocardia-like ST46 is smoked, the flower fragrance and the sweet fragrance in the smoke are obvious, the richness of the smoke fragrance is obviously increased, the moisture feeling of the smoke is improved, the miscellaneous gas is reduced, and the smoking quality of the cigarette is obviously improved.
In terms of color change, the control cigarette 1# prepared without any fermentation therein was pale yellow, low in chroma, and changed in color but not significantly during smoking, and did not become a pure violet blue color. The starting colors of the control cigarette 2# and the control cigarette 3# are greyish yellow; as the suction process proceeds, the color gradually turns blue-brown, the tone is dark, and the visual effect is not prominent enough. The color-changeable violet perfume applied to the test cigarettes has strong tinting strength, the initial color is bright yellow, and the color tone is vivid and natural; the color gradually changes into bright purple blue along with the suction process; the color change difference is obvious, and the visual effect is outstanding.
Table 1 functional evaluation of color cigarette paper coating liquids
Example 3: the use of the color-changeable violet perfume, violet extract obtained in example 1, comparative example 2 and comparative example 3 in tipping paper comprises the following steps:
(1) Preparation of color-changeable violet spice ink
Taking the color-changeable violet perfume and the violet extract prepared in the example 1, the comparative example 2 and the comparative example 3, respectively diluting the color-changeable violet perfume and the violet extract with ethanol for 2 times, and then dripping a citric acid saturated solution until the pH value is about 4.5, wherein the color of the color-changeable violet perfume solution is purple; mixing 10 parts of fungal polysaccharide, 60 parts of propylene glycol and 30 parts of ethanol for 2 hours, adding 0.3wt% of leveling agent polyacrylate of the mass of the mixed solution, and mixing uniformly to obtain four parts of ink matrix; then respectively mixing the mauve color-changeable violet perfume solution and the ink matrix according to the proportion of 1: mixing uniformly in a mass ratio of 1 to obtain the four color-changeable violet spice inks.
(2) Preparation and application of color tipping paper containing color-changeable violet perfume
In the tipping paper printing process, the four kinds of color-changeable violet perfume ink are printed on the tipping paper by adopting intaglio printing, so that the color tipping paper containing the color-changeable violet perfume is obtained, and then the tipping paper is further rolled into cigarettes.
(3) Evaluation results
The results are substantially the same as in example 2 in terms of olfactory note. In terms of color change, the patterns on the tipping paper are all reddish, the cigarettes are lighted for smoking, and after the smoke passes through the filter tip, the patterns on the tipping paper are gradually changed into purplish blue. Only the patterns on the tipping paper of the test cigarettes start to be bright and pure purple, and the colors gradually change into bright and pure purple blue along with the suction process; the color change is bright and the visual effect is prominent. The pattern on the tipping paper obtained in comparative example 1 has a bright and pure color, and the color is light, and gradually changes into gray blue along with the suction process; the patterns on the tipping paper obtained in comparative examples 2 and 3 are not bright and pure enough in initial color and dark in color, and the color gradually changes to blue-brown along with the suction process; comparative examples 1, 2 and 3 are not bright and pure enough in color and are not outstanding in visual effect.
Example 4: the application of the color-changeable violet perfume in the cigarette filter rod particles specifically comprises the following steps:
the color-changeable violet perfume and violet extract prepared in example 1, comparative example 2 and comparative example 3 were respectively added dropwise with tartaric acid solution to make the perfume color changed to purplish red, and curcumin was continuously added to the purplish red perfume solution to obtain four purplish red color-changeable violet perfumes. The four violet spice are respectively frozen and dried under the conditions that the freezing temperature is-70 ℃, the hydrazine cooling temperature is-10 ℃, the vacuum degree is 15Pa and the final freeze-drying temperature is 20 ℃ to obtain four color-changeable violet spice freeze-dried powder; according to a spray granulation process, the four color-changeable violet spice freeze-dried powders and soluble starch are mixed according to a mass ratio of 1:4, after evenly mixing, placing the mixture in a fluidized bed, and spraying an adhesive solution with the mass of 5wt% of the freeze-dried powder of the color-changeable violet spice during granulation to obtain four color-changeable violet spice particles.
In the process of forming the composite cigarette filter stick, the four prepared color-changeable violet spice particles are applied to the filter stick filament bundle according to the amount of 2mg per millimeter of the filter stick, or are intensively added to the transparent cavity part of the composite filter stick, so that four cigarette filter sticks containing the color-changeable violet spice particles are obtained, and then the cigarette filter sticks are further rolled into cigarettes and evaluated.
The smell results were substantially the same as in example 2. In terms of color change, the appearance of the prepared four kinds of color-changeable violet perfume-containing particles is orange, and when the color-changeable violet perfume-containing particles are added into a filter rod to prepare a cigarette, the cigarette is lighted for smoking, and smoke passes through the filter rod, and filter rod particles in a transparent cavity or among filter rod tows are gradually green. Only the particle color of the test cigarette is bright and pure orange, and the color gradually changes into bright and pure grass green along with the suction process; the color change is bright and the visual effect is prominent. The granules obtained in comparative example 1 were not bright and pure enough in starting color, were pale in color, and gradually changed to yellowish green as the suction process was carried out; the patterns on the tipping paper obtained in comparative examples 2 and 3 are not bright and pure enough in initial color and dark in color, and the colors gradually change into grey green along with the suction process; comparative examples 1, 2 and 3 are not bright and pure enough in color and are not outstanding in visual effect.
Example 5: the application of the color-changeable violet perfume in the cigarette tows specifically comprises the following steps:
the color-changeable violet perfume and violet extract prepared in example 1, comparative example 2 and comparative example 3 are respectively added with sodium carbonate solution dropwise until the pH value is 7.5, the color of the solution is yellow, and then the solution is frozen and dried under the conditions that the freezing temperature is-60 ℃, the cold hydrazine temperature is-20 ℃, the vacuum degree is 20Pa, and the freeze-drying final temperature is 25 ℃ to obtain four yellow color-changeable violet perfume freeze-dried powders.
(1) After the acetate fiber master batch acetate diacetic tablet is made into slurry, 1 weight percent of the four freeze-dried powders containing the color-changeable violet perfume are respectively added and uniformly mixed, four yellow tows containing the color-changeable violet perfume are formed through a tow production process, a filter tip containing the color-changeable violet perfume is formed through a filter tip forming device, and then four cigarettes are further rolled. When the cigarette is lit for smoking, the smoke passes through the filter rod and the color of the filter rod tows changes. The smell results were substantially the same as in example 2. The color change was from yellowish to purplish blue, and the four cigarettes were substantially the same as in example 2.
(2) The four color-changeable violet spice freeze-dried powders are respectively added with a small amount of ethanol for dilution and dissolution, then added into plasticizer triacetin according to the proportion of 2wt%, so as to obtain four color-changeable violet spice-containing meta-yellow triacetin, and the four color-changeable violet spice-containing meta-yellow triacetin is sprayed into a common tow through a filter tip forming device to form four color-changeable violet spice-containing filter tips, and then further rolled into cigarettes. The smell results were substantially the same as in example 2. The color change was from yellowish to purplish blue, and the four cigarettes were substantially the same as in example 2.
It will be readily appreciated by those skilled in the art that the foregoing description is merely a preferred embodiment of the invention and is not intended to limit the invention, but any modifications, equivalents, improvements or alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.
Claims (4)
1. Use of a fermented flavour in a cigarette material; the cigarette material is characterized by comprising cigarette paper, tipping paper, filter rod particles and tows; the fermented spice is purple blue clear liquid, and the solid content of the fermented spice is 60-80 wt%; the preparation method of the fermented spice comprises the following steps:
(1) Grinding, crushing and sterilizing fresh violet petals to obtain violet slurry, fermenting for a period of time by using nocardia-like ST46 microbial inoculum, and sterilizing to obtain violet fermentation slurry;
(2) Performing microwave extraction on the violet fermentation slurry obtained in the step (1) by using 5-10 times of absolute ethyl alcohol, separating an extracting solution, and concentrating the obtained filtrate under reduced pressure until the volume is not changed, thus obtaining a crude extracting solution;
(3) Loading and adsorbing the crude extract obtained in the step (2) by using a silica gel chromatographic column, and then sequentially carrying out gradient elution by using 30wt% ethanol, 50wt% ethanol, 70wt% ethanol and 90wt% ethanol solutions with the volume of 3-5 times of the column volume; collecting and combining 50wt% and 90wt% ethanol solution eluates;
(4) Concentrating under reduced pressure to obtain color-changeable violet perfume, which is the fermented perfume.
2. The use according to claim 1, wherein the nocardia ST 46-like agent of step (1) is prepared by the following method: every 1000mL of nocardia like ST46 fermentation culture solution subjected to expansion fermentation culture is centrifuged at 3000rpm for 10min, the precipitate is washed by sterile water, and finally the precipitate is uniformly oscillated by 20mL sterile water, and the nocardia like ST46 microbial inoculum is obtained after 10 times dilution during use.
3. The use according to claim 1, wherein the nocardia ST 46-like agent of step (1) is fermented in the following steps: inoculating 10-20 mL of nocardia ST46 bacteria agent into each 100g of violet pulp, fermenting for 24-72 h, and sterilizing in a sterilizing pot at 121 ℃ for 30min to obtain violet fermentation pulp.
4. Use according to claim 1, characterized in that the application method steps are as follows:
(1) In cigarette paper: diluting the color-changeable violet spice with ethanol for 2-5 times; mixing 5-15 parts of fungal polysaccharide, 55-65 parts of propylene glycol and 25-35 parts of ethanol uniformly by shaking for 1-5 hours to obtain a coating liquid matrix; uniformly mixing the diluted color-changeable violet perfume with a coating liquid matrix according to the mass ratio of 1:1 to obtain color-changeable violet perfume coating liquid; coating the color-changeable violet spice coating liquid on the cigarette paper by adopting a spray coating, roll shaft embossing or fixed-point spray printing mode to obtain color cigarette paper containing the color-changeable violet spice;
(2) In tipping paper: diluting the color-changeable violet spice with ethanol for 2-5 times; mixing 5-15 parts of fungal polysaccharide, 55-65 parts of propylene glycol and 25-35 parts of ethanol for 1-5 hours, and adding an ink auxiliary agent accounting for 0.1-1wt% of the mass of the mixed solution to obtain an ink matrix; uniformly mixing the diluted color-changeable violet perfume with an ink matrix according to the mass ratio of 1:1 to obtain color-changeable violet perfume ink; printing on the tipping paper in a printing mode of gravure printing, flexography, roller shaft embossing or inkjet printing to obtain color tipping paper containing the color-changeable violet perfume;
(3) In the cigarette filter particles: freeze-drying the color-changeable violet spice to obtain color-changeable violet spice freeze-dried powder; uniformly mixing the obtained spice freeze-dried powder and a granule excipient according to the mass ratio of 1:1-1:10, and then placing the mixture in a fluidized bed for spray granulation, wherein an adhesive solution with the concentration of 4-10wt% is sprayed in the granulation process, so as to obtain the color-changeable violet spice granules; adding the obtained color-changeable violet spice particles into a transparent cavity of a multi-element composite filter rod or a filter rod silk bundle gap;
(4) In cigarette tows: freeze-drying the color-changeable violet spice to obtain color-changeable violet spice freeze-dried powder; uniformly mixing the spice freeze-dried powder with the fiber master batch according to the mass ratio of 0.1-3 wt% of the fiber master batch of the tows, and forming color tows containing the color-changeable violet spice through a tow production process and a filter tip forming process; or diluting the color-changeable violet spice freeze-dried powder with ethanol, adding the diluted color-changeable violet spice freeze-dried powder into the plasticizer glyceryl triacetate according to the proportion of 1-5 wt%, and spraying the plasticizer glyceryl triacetate into the tows through a filter tip forming device to form the color filter tip containing the color-changeable violet spice.
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