CN105918547B - A kind of agaricus bisporus sea-buckthorn instant tea and preparation method thereof - Google Patents
A kind of agaricus bisporus sea-buckthorn instant tea and preparation method thereof Download PDFInfo
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- CN105918547B CN105918547B CN201610264208.3A CN201610264208A CN105918547B CN 105918547 B CN105918547 B CN 105918547B CN 201610264208 A CN201610264208 A CN 201610264208A CN 105918547 B CN105918547 B CN 105918547B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
Abstract
The present invention provides a kind of agaricus bisporus sea-buckthorn instant teas, belong to instant tea product processing technique field, the present invention provides a kind of agaricus bisporus sea-buckthorn instant tea by agaricus bisporus Aqueous extracts and Leaves of Hippophae L Aqueous extracts by mixing, the powdered agaricus bisporus sea-buckthorn instant tea that concentration, coronoid process dissipate capsule bacterium and Aspergillus niger liquid state fermentation, filtering, vacuum drying are prepared.Simultaneously, the present invention also provides a kind of preparation methods of agaricus bisporus sea-buckthorn instant tea, specifically include: water mentions Leaves of Hippophae L, water extracts and filters by pretreated agaricus bisporus and mixed extract, concentration, UHT sterilization, preparation coronoid process dissipate capsule bacterium and aspergillus niger seed liquor, successive secondary inoculation mixed fungus fermentation, centrifugation, film, is dried to obtain agaricus bisporus sea-buckthorn instant tea.The present invention not only increases the fermentation efficiency of bacterial strain, eliminates the bitter taste in sea-buckthorn tea, and remains the mannose in agaricus bisporus, trehalose and various amino acids substances, and nutrient composition content is high, and preparation method is suitble to be mass produced.
Description
Technical field
The invention belongs to instant sea-buckthorn tea manufacture fields, and in particular to a kind of agaricus bisporus sea-buckthorn instant tea and its preparation side
Method.
Background technique
The bacterial context fertilizer of agaricus bisporus is tender, and contains more mannose, trehalose and various amino acids substances, so taste
It is delicious, full of nutrition, 36-40g containing protein, fat 3.6g, carbohydrate 31.2g, phosphorus in the dry mushroom of every 100g according to surveying and determination
718mg, iron 188.5mg, calcium 131mg, ash content 14.2mg, crude fibre 6g additionally contain vitamin B1, B2, Vc, niacin
Deng since its nutrition is higher than general vegetables, so there is the title of " Vegetable meat ".
Sea-buckthorn (Classification system: Hippophae rhamnoides Linn.) is a kind of fallen leaves property shrub, and characteristic is resistance to
Drought, anti-blown sand, can survive, therefore be widely used in water and soil conservation on wetland with saline-alkaline.The root of sea-buckthorn, stem, leaf, flower, fruit
Nutriment and bioactive substance all rich in, wherein Leaves of Hippophae L has the function of a variety of medicine, and can invigorate blood circulation decompression, right
The all diseases of blood and sweat guard system have significant treatment to act on.The a large amount of plantation sea-buckthorns in northwest China area, are used for desert afforestation.In order to guarantee
The growth of sea-buckthorn, Leaves of Hippophae L are usually that can just harvest in summer and autumn, and Leaves of Hippophae L is mature at this time, even more thick old,
In addition it is usually taken after drying or drying as tea-drinking, flavor is not mature, thick old highly seasoned, and consumer is often difficult to receive.Therefore,
In order to make full use of Leaves of Hippophae L resource, the flavor of hippophae rhamnoides tea is needed to be improved, a kind of new hippophae rhamnoides tea product is researched and developed.
Applicant's retrieval, the prior art of the relevant processing method of hippophae rhamnoides tea are as follows: application No. is
A kind of production method of 201410766432.3 golden flower hippophae rhamnoides tea is passed through on the basis of hippophae rhamnoides tea original technique
What biotechnology improved, i.e., it is respectively connected to a certain amount of coronoid process dissipate capsule bacterium strain after " just steaming " and " multiple to steam ", is connect to be secondary
Bacterium method makes when pile-fermentation coronoid process dissipate capsule bacterium rise to dominant bacteria respectively, and hair " golden flower " is luxuriant, universal when ageing.Above-mentioned two
A invention invention is to carry out solid fermentation, the hippophae rhamnoides tea of processing and fermentation on microbial inoculant to Leaves of Hippophae L.
The sea-buckthorn instant tea and production method of application number CN03125119.6 is that Leaves of Hippophae L is finished, rubs and fried
It is dry, using adding boiling water to impregnate, extract, filtering, concentration, dry, modulation and processing and forming process.
The sea-buckthorn instant tea and preparation method thereof of application number 200710000434.1, this method include by collected sea-buckthorn
Extracted dose of leaf is extracted, is concentrated, and is obtained concentrated extracting solution, is spray-dried to concentrated extracting solution, obtains the sand of powdery, water-soluble
The sea-buckthorn leaf extract and auxiliary material are deployed in required ratio, the instant sea-buckthorn of required flavor and taste are made by spine leaf extract
Tea.
The safflower seabuckthorn freeze-dried instant powder of application number 201510054854.2 is to be with safflower suede, sea-buckthorn processing disintegrating slag
Raw material does effective component in extraction media safflower extracting using water, and is refined by purifying, concentration to it, uses ultrasonic wave
Assisted recombination enzyme extraction method extracts the effective component in sea-buckthorn branches and leaves disintegrating slag, and carries out concentrating and impurity removing to it, using vacuum refrigeration
Method is dried, and finally carries out exquisiteness into instant tea powder using superfine communication technique.Above technique uses traditional handicraft
The effective component that Leaves of Hippophae L is extracted with traditional Extraction solvent, is then processed into instant Leaves of Hippophae L for the effective component extracted
Tea.
Hippophae rhamnoides tea flavor is not mature, thick old highly seasoned, and consumer is often difficult to receive, and instant tea is also inventor's discovery
After agaricus bisporus is added in Leaves of Hippophae L, using aspergillus niger mixed fermentation, and after being dried to instant tea, the tea taste of Leaves of Hippophae L has aobvious
It writes and changes, it is not only delicious soft, and also the effect fermented also is significantly increased, and therefore, it is husky that the invention proposes a kind of agaricus bisporus
Spine leaf instant tea and preparation method thereof.
Summary of the invention
The present invention provides a kind of mannose remained in agaricus bisporus, trehalose and various amino acids substances, nutrition
Abundant, the agaricus bisporus sea-buckthorn instant tea of delicious flavour, the agaricus bisporus sea-buckthorn instant tea is specifically by agaricus bisporus Aqueous extracts and sand
After the mixing of spine leaf Aqueous extracts, by coronoid process dissipate capsule bacterium and aspergillus niger mixed fungus fermentation, and powdered agaricus bisporus made of being dried in vacuo
Sea-buckthorn instant tea.
Meanwhile the present invention also provides a kind of preparation method of agaricus bisporus sea-buckthorn instant tea, specifically comprise the following steps:
A. water mentions Leaves of Hippophae L and agaricus bisporus: extracting Leaves of Hippophae L using 75~95 DEG C of water, obtains Leaves of Hippophae L Aqueous extracts, uses
80~90 DEG C of water, which extracts, passes through pretreated agaricus bisporus, and filtering, centrifugation obtain agaricus bisporus Aqueous extracts；
B. agaricus bisporus sea-buckthorn mixed liquor is concentrated: agaricus bisporus Aqueous extracts and Leaves of Hippophae L Aqueous extracts are mixed, obtains agaricus bisporus sand
Spine mixed liquor is concentrated agaricus bisporus sea-buckthorn mixed liquor to the 10%~30% of original volume, obtains agaricus bisporus sea-buckthorn concentrate；
C. it sterilizes: agaricus bisporus sea-buckthorn concentrate being sterilized using UHT method for disinfection；
D. seed liquor is cultivated: using PDA culture medium activation culture coronoid process dissipate capsule bacterium and aspergillus niger respectively, then with agaricus bisporus sea-buckthorn
Mixed liquor is cultivated step by step, prepares 3 grades of coronoid process dissipate capsule bacterium and 2 grades of seed liquor seed liquors of aspergillus niger；
E. it is inoculated with: 3 grades of seed liquors of coronoid process dissipate capsule bacterium being inoculated into agaricus bisporus sea-buckthorn mixed liquor, in the fermenter for the first time
Aerobic 48~120h of liquid state fermentation；
F. it is inoculated with for second: accessing 2 grades of seed liquors of aspergillus niger in the fermenter, continue aerobic 72~144h of liquid state fermentation；
G. centrifugation and film filtering, remove the impurity in fermentation liquid；
H. vacuum drying obtains agaricus bisporus sea-buckthorn instant tea.
Further, described to extract Leaves of Hippophae L using 75~95 DEG C of water specifically: to be extracted using 75~95 DEG C of softened waters husky
Spine leaf, solid-liquid ratio are 1:10~1:25；Extraction time 40-50min, filters to take filtrate, obtains Leaves of Hippophae L Aqueous extracts；
Further, the water using 80~90 DEG C is extracted is specially by pretreated agaricus bisporus；Using 80~90 DEG C,
The softened water that solid-liquid ratio is 1:10~1:20 extracts 40~50min of agaricus bisporus after pretreatment, obtains agaricus bisporus Aqueous extracts；
Further, in the step B, the ratio that agaricus bisporus Aqueous extracts and Leaves of Hippophae L Aqueous extracts are mixed is in 1:4~1:14
Between.
Further, the temperature of the aerobic liquid state fermentation is 28~32 DEG C.
Further, in the first time inoculation, the inoculum concentration of 3 grades of seed liquors of coronoid process dissipate capsule bacterium is 3%~10% (v/v)；
In second of inoculation, the inoculum concentration of 2 grades of seed liquors of aspergillus niger is 2%~8% (v/v).
Further, the centrifugation and film filtering specifically: 400 mesh filter screen filtrations of the liquid after fermentation are removed into mycelia
Body, centrifugation are that permeate is collected by filtration in 0.2 micrometer ceramics film with aperture, obtain clear agaricus bisporus sea-buckthorn fermentation liquid.
Beneficial effect
First, the present invention mixes agaricus bisporus and Leaves of Hippophae L mixed liquor by 2 plants of strains of coronoid process dissipate capsule bacterium and aspergillus niger
Bacterium fermentation prepares instant tea, wherein agaricus bisporus and Leaves of Hippophae L mixed fungus fermentation not only effectively improve hippophae rhamnoides tea bitterness originally
Equal bad flavors increase fermentation rate, and fermentation also adds the nutritional ingredient ingredient in instant tea.After testing, add
Add the dry mycelial weight of agaricus bisporus high 1 times or more more universal than the dry mycelial weight for not adding agaricus bisporus, the polyphenol content in fermentation liquid is aobvious
Writing reduces, and the bitter taste in instant tea is mainly derived from the polyphenol substance of fermentation liquid, makes the bitterness sense in instant tea aobvious in this way
Decline is write, after the present invention adds agaricus bisporus, the free aminoacid content in fermentation liquid significantly increases, it may be assumed that instant tea brews rear ammonia
Base acid content significantly rises, it is well known that amino acid is mainly at taste ingredient, i.e., it is fresh that instant tea prepared by the present invention brews taste
Beauty.
Applicant, which compares a variety of fungi constituents and is added in Leaves of Hippophae L, ferments, such as: tremella, agaric and needle mushroom,
It was found that having agaricus bisporus only not only and can be significantly reduced the polyphenol substance in fermentation liquid, the free amino acid increased in fermentation liquid contains
Amount makes instant tea brew rear delicious flavour, and fermentation liquid clear, and color is red dense bright, and the present invention can promote coronoid process
The fermentation of bulk bacteria and aspergillus niger increases its dry mycelial weight by 1 times or more, more than the ferment effect that equivalent glucose is added.
If applicant will access in agaricus bisporus sea-buckthorn culture medium simultaneously into coronoid process dissipate capsule bacterium and aspergillus niger, due to bacterium
Competition mechanism between strain, the agaricus bisporus sea-buckthorn instant tea that the growth of coronoid process dissipate capsule bacterium is suppressed, while fermenting and preparing
Taste and flavor is poor.
Also containing nutrition such as mannose, trehalose and various amino acids substances in agaricus bisporus sea-buckthorn instant tea of the invention
Ingredient can make the faint scent for having pleasant in instant tea without adding the additives such as sucrose, glucose, essence just.
At the same time, agaricus bisporus sea-buckthorn instant tea of the invention also has activating microcirculation and removing stasis medicinal, relax bowel and defecation, adjustment enteron aisle thin
Bacterium, beneficial stomach, tonifying Qi and blood, heart tonifying, strong body, cerebrum tonifying, refresh oneself etc. traditional sea-buckthorn tea and dark green tea the effect of.With good health
Health-care effect.
Second, the present invention is the mixed fungus fermentation for carrying out coronoid process dissipate capsule bacterium and aspergillus niger in the fermenter, mixed fungus fermentation makes
The color, smell and taste of product are more sufficient, meanwhile, the safety of product can be improved.Preparation process of the invention is simple, to Zymolysis Equipment
Of less demanding, fermentation costs are low, are suitable for industrialization large-scale production.
Specific embodiment
The present invention is furture elucidated for son combined with specific embodiments below, it should be understood that these embodiments are merely to illustrate this hair
It is bright, rather than limit the scope of the invention, after the present invention has been read, those skilled in the art is various to the present invention etc.
The modification of valence form falls within the range of the application appended claims defined.
In embodiment, coronoid process dissipate capsule bacterium used and Aspergillus niger strain are provided by Agricultural University Of Anhui, and coronoid process dissipate capsule bacterium is protected
Hiding number is CGMCC No.10610, and the deposit number of aspergillus niger is CGMCC No.11276.But it is prominent that the present invention is not limited to this two canopies
Bulk bacteria and Aspergillus niger strain, it is all to belong to coronoid process dissipate capsule bacterium and the bacterial strain of aspergillus niger subspecies is suitable for the present invention.
Embodiment 1
A kind of agaricus bisporus sea-buckthorn instant tea, preparing raw material mainly includes Leaves of Hippophae L and agaricus bisporus, and specific procedure of processing is as follows:
Leaves of Hippophae L pretreatment: taking 200 kilograms of Leaves of Hippophae L dried, be put into extractor after pulverizer crushes, and is added
2600 kilograms 75 DEG C of softened water, it may be assumed that solid-liquid ratio 1:13, the soak extraction 50 minutes under the conditions of 95 DEG C；
Filtering Leaves of Hippophae L: the strainer that the solution after immersion is first crossed to 40 mesh removes slag, and the strainer after 100 mesh removes residue, obtains
To Leaves of Hippophae L Aqueous extracts.
Agaricus bisporus slurrying: taking dry agaricus bisporus to remove part of rotting, accurately weigh 10 kilograms of processed dry agaricus bisporus,
It cleans, after beater is beaten, 200 kilograms 90 DEG C of softened water is added, is i.e. solid-liquid ratio is 1:20, impregnates and mentions under the conditions of 85 DEG C
It takes 50 minutes；
The filtering of agaricus bisporus solution: the strainer that the agaricus bisporus solution after immersion is first crossed to 200 mesh removes slag, at 3000r/min
Centrifugation, obtains agaricus bisporus Aqueous extracts.
Mixing: agaricus bisporus Aqueous extracts are mixed with Leaves of Hippophae L Aqueous extracts according to 1:14 (v/v), obtain the mixing of agaricus bisporus sea-buckthorn
Liquid.
Concentration: agaricus bisporus sea-buckthorn mixed liquor being squeezed into vacuum concentration equipment, the 30% of original solution is concentrated at 70 DEG C,
Obtain agaricus bisporus sea-buckthorn concentrate.
Sterilization: by agaricus bisporus sea-buckthorn concentrate and agaricus bisporus concentrate, crossing UHT, sterilize 125 DEG C of parameter, 20 seconds, then will
Agaricus bisporus sea-buckthorn concentrate is squeezed into the fermentor after sterilization processing.
Seed liquor is cultivated: being used deposit number to cultivate in PDA culture medium 6 days for the coronoid process dissipate capsule bacterium of CGMCC10610, is made
Strain is activated.It will be cultivated in strain access agaricus bisporus sea-buckthorn concentrate after activation, obtain 1 grade of seed of coronoid process dissipate capsule bacterium
Liquid；1 grade of seed liquor of coronoid process dissipate capsule bacterium is inoculated into agaricus bisporus sea-buckthorn mixed liquor culture, obtains 2 grades of seed liquors of coronoid process dissipate capsule bacterium,
Same method, culture obtains 3 grades of seed liquors of coronoid process dissipate capsule bacterium step by step；Similarly, use deposit number for the black song of CGMCC11276
Mould culture step by step obtains 2 grades of seed liquors of aspergillus niger.
It is inoculated with for the first time: 3 grades of seed liquors of coronoid process dissipate capsule bacterium is inoculated into the fermentor equipped with agaricus bisporus sea-buckthorn concentrate,
Inoculum concentration 8.0 (v/v) %；
Aerobic liquid state fermentation: it controls 30 DEG C of agaricus bisporus Leaves of Hippophae L broth temperature in fermentor and is passed through filtrated air, send out
Ferment 72 hours；
Second of inoculation: 2 grades of seed liquors of aspergillus niger are inoculated into the fermentor of the agaricus bisporus sea-buckthorn concentrate, are inoculated with
6.0 (v/v) % are measured, are continued aerobic fermentation 96 hours；
Centrifugation and film filtering: removing mycelium for the strainer that the liquid after fermentation crosses 400 mesh, after butterfly centrifugal machine, from
Scheming revolving speed is 4500 revs/min, obtains more clear centrifugate, then squeezing into membrane aperture is in 0.2 micrometer ceramics film device
Film process is carried out, ceramic membrane permeate is collected, obtains the agaricus bisporus sea-buckthorn tea fermentation liquid of clear.
Freeze-drying: it is quick-frozen that agaricus bisporus sea-buckthorn fermentation liquid is subjected to paving disk, and is freeze-dried, dry double spores are obtained
Mushroom sea-buckthorn instant tea.
Using the method for the present embodiment, 28.9 kilograms of agaricus bisporus sea-buckthorn instant teas are made altogether.Instant tea of the invention carries
It is convenient, change the problem that traditional hippophae rhamnoides tea is bitter, mouthfeel is not positive, brew rear Chen Xiang higher, flavour alcohol just, soup look glow,
Also contain a large amount of active nutrient components in instant tea.
Applicant determines the parameter during the agaricus bisporus sea-buckthorn instant tea mixed fungus fermentation using embodiment 1, wherein
Dry mycelial weight uses: fermentation liquid being filtered, the mycelium pure water rinsing left through 2 layer of 400 mesh silk, then will
Mycelium places 105 DEG C of baking ovens, and drying to constant weight, measures its dry biomass, i.e. dry mycelial weight with assay balance.
The measuring method of polyphenol content: Folin-Ciocalteu method.
Free amino acid measuring method: ninhydrin.
Flavones content: alchlor colorimetric method.
Turbidity: it is measured using Turb555/Turb555IR high-precision transmissometer.
The variation of 1 different culture medium of table fermentation different time dry mycelial weight
As it can be seen from table 1 the dry mycelial weight of addition agaricus bisporus is generally more much higher than the dry mycelial weight for not adding agaricus bisporus,
After adding agaricus bisporus, the effective component of agaricus bisporus provides nutrition and growth factor for coronoid process dissipate capsule bacterium and aspergillus niger, so that 2
It is grown comparatively fast during strain strain fermentation, mycelia is more, and enzyme activity is higher.
The variation of 2 different culture medium of table fermentation different time fermentation liquid polyphenol content
Table 2 has reacted the polyphenol content in fermentation liquid, and polyphenol content directly affects bitterness sense in fermented tea.Although polyphenol has
Certain anti-oxidation function, however the higher bitterness sense that will increase fermented tea of polyphenol content.Adding certain agaricus bisporus can promote
Into coronoid process dissipate capsule bacterium and Aspergillus Niger Growth, increase polyphenol oxidase content, while reducing polyphenol content.
The variation of 3 different culture medium of table fermentation different time fermentation liquid free aminoacid content
Amino acid has certain nutritive value first, and secondly free amino acid has certain nutrition to the mouthfeel of fermented tea,
The content height of free amino acid can increase the fermentation fresh and sweet taste of millet paste, with the quality of millet paste at the positive correlation of height.It can be with from table 3
Find out, due to double born of the same parents mushroom amino acid rich in, after adding agaricus bisporus, the amino acid content of fermentation liquid is higher, the product of millet paste
Matter is high.
Flavones content and turbidity value after 4 different culture medium of table is fermented 8 days
Flavone compound is a kind of important biological active agents, has very strong oxidation resistance, has simultaneously
The pharmacological actions such as antiulcer, anticancer, anti-diabetic complication.Flavones content height illustrates its oxidation resistance height.Add agaricus bisporus
Afterwards, the flavones content of agaricus bisporus sea-buckthorn instant tea is relatively high, and biological activity is more preferable.
Turbidity is the important indicator of evaluation fermentation millet paste sense organ, as can be seen that not shadow after addition agaricus bisporus from data
Its turbidity is rung, is had no adverse effects to fermentation.
Embodiment 2
A kind of agaricus bisporus sea-buckthorn instant tea, preparing raw material mainly includes Leaves of Hippophae L, agaricus bisporus, and specific procedure of processing is as follows:
Leaves of Hippophae L pretreatment: taking 200 kilograms of Leaves of Hippophae L dried, be put into extractor after pulverizer crushes, and is added
2000 kilograms 95 DEG C of softened water, it may be assumed that solid-liquid ratio 1:10, the soak extraction 40 minutes under the conditions of 95 DEG C；
Filtering Leaves of Hippophae L: the strainer that the solution after immersion is first crossed to 40 mesh removes slag, and the strainer after 100 mesh removes residue, obtains
To Leaves of Hippophae L Aqueous extracts.
Agaricus bisporus slurrying: taking dry agaricus bisporus to remove part of rotting, accurately weigh 10 kilograms of processed dry agaricus bisporus,
It cleans, after beater is beaten, 100 kilograms 80 DEG C of softened water is added, is i.e. solid-liquid ratio is 1:10, impregnates and mentions under the conditions of 80 DEG C
It takes 40 minutes；
The filtering of agaricus bisporus solution: the strainer that the agaricus bisporus solution after immersion is first crossed to 200 mesh removes slag, at 4000r/min
Centrifugation, obtains agaricus bisporus Aqueous extracts.
Mixing: agaricus bisporus Aqueous extracts are mixed with Leaves of Hippophae L Aqueous extracts according to 1:4 (v/v), obtain the mixing of agaricus bisporus sea-buckthorn
Liquid.
Concentration: agaricus bisporus sea-buckthorn mixed liquor is filled into concentrator, the 10% of original solution is concentrated at 70 DEG C, is obtained
Agaricus bisporus sea-buckthorn concentrate.
Sterilization: by agaricus bisporus sea-buckthorn concentrate and agaricus bisporus concentrate, crossing UHT, sterilize 135 DEG C of parameter, 30 seconds, then will
Agaricus bisporus sea-buckthorn concentrate is squeezed into the fermentor after sterilization processing.
Seed liquor is cultivated: being used deposit number to cultivate in PDA culture medium 6 days for the coronoid process dissipate capsule bacterium of CGMCC10610, is made
Strain is activated.It will be cultivated in strain access agaricus bisporus sea-buckthorn concentrate after activation, obtain 1 grade of seed of coronoid process dissipate capsule bacterium
Liquid；1 grade of seed liquor of coronoid process dissipate capsule bacterium is inoculated into agaricus bisporus sea-buckthorn mixed liquor culture, obtains 2 grades of seed liquors of coronoid process dissipate capsule bacterium,
Same method, culture obtains 3 grades of seed liquors of coronoid process dissipate capsule bacterium step by step；Similarly, use deposit number for the black song of CGMCC11276
Mould culture step by step obtains 2 grades of seed liquors of aspergillus niger.
It is inoculated with for the first time: 3 grades of seed liquors of coronoid process dissipate capsule bacterium is inoculated into the fermentor equipped with agaricus bisporus sea-buckthorn concentrate,
Inoculum concentration 3.0 (v/v) %；
Aerobic liquid state fermentation: it controls 32 DEG C of agaricus bisporus Leaves of Hippophae L broth temperature in fermentor and is passed through filtrated air, send out
Ferment 120 hours；
Second of inoculation: 2 grades of seed liquors of aspergillus niger are inoculated into the fermentor of the agaricus bisporus sea-buckthorn concentrate, are inoculated with
2.0 (v/v) % are measured, are continued aerobic fermentation 144 hours；
Centrifugation and film filtering: removing mycelium for the strainer that the liquid after fermentation crosses 400 mesh, after butterfly centrifugal machine, from
Scheming revolving speed is 4500 revs/min, obtains more clear centrifugate, then squeezing into membrane aperture is in 0.2 micrometer ceramics film device
Film process is carried out, ceramic membrane permeate is collected, obtains the agaricus bisporus sea-buckthorn tea fermentation liquid of clear.
Freeze-drying: by agaricus bisporus sea-buckthorn tea fermentation liquid carry out paving disk it is quick-frozen and freeze-drying, obtain agaricus bisporus sea-buckthorn speed
Molten tea.Through detecting, the present embodiment altogether be made 28.9 kilograms brew after give off a strong fragrance, flavour is mellow, soup look is isabelline, clarification is saturating
It is bright.
Embodiment 3
A kind of agaricus bisporus sea-buckthorn instant tea, preparing raw material mainly includes Leaves of Hippophae L, agaricus bisporus, and specific procedure of processing is as follows:
Leaves of Hippophae L pretreatment: taking 150 kilograms of Leaves of Hippophae L dried, be put into extractor after pulverizer crushes, and is added
3750 kilograms 95 DEG C of softened water, it may be assumed that solid-liquid ratio 1:25, the soak extraction 40 minutes under the conditions of 95 DEG C；
Filtering Leaves of Hippophae L: the strainer that the solution after immersion is first crossed to 40 mesh removes slag, and the strainer after 100 mesh removes residue, obtains
To Leaves of Hippophae L Aqueous extracts.
Agaricus bisporus slurrying: taking dry agaricus bisporus to remove part of rotting, accurately weigh 10 kilograms of processed dry agaricus bisporus,
It cleans, after beater is beaten, 100 kilograms 85 DEG C of softened water is added, is i.e. solid-liquid ratio is 1:10, impregnates and mentions under the conditions of 85 DEG C
It takes 40 minutes；
The filtering of agaricus bisporus solution: the strainer that the agaricus bisporus solution after immersion is first crossed to 200 mesh removes slag, at 3000r/min
Centrifugation, obtains agaricus bisporus Aqueous extracts.
Mixing: agaricus bisporus Aqueous extracts are mixed with Leaves of Hippophae L Aqueous extracts according to 1:4 (v/v), obtain the mixing of agaricus bisporus sea-buckthorn
Liquid.
Concentration: agaricus bisporus sea-buckthorn mixed liquor being squeezed into vacuum concentration equipment, the 20% of original solution is concentrated at 70 DEG C,
Obtain agaricus bisporus sea-buckthorn concentrate.
Sterilization: by agaricus bisporus sea-buckthorn concentrate and agaricus bisporus concentrate, crossing UHT, sterilize 135 DEG C of parameter, 30 seconds, then will
Agaricus bisporus sea-buckthorn concentrate is squeezed into the fermentor after sterilization processing.
Seed liquor is cultivated: being cultivated 6 days in PDA culture medium using coronoid process dissipate capsule bacterium, is activated strain.After activating
Strain access agaricus bisporus sea-buckthorn concentrate in cultivate, obtain 1 grade of seed liquor of coronoid process dissipate capsule bacterium；By 1 grade of seed of coronoid process dissipate capsule bacterium
Liquid inoculates agaricus bisporus sea-buckthorn mixed liquor culture, obtains 2 grades of seed liquors of coronoid process dissipate capsule bacterium, same method, culture obtains step by step
Obtain 3 grades of seed liquors of coronoid process dissipate capsule bacterium；Similarly, it is cultivated step by step using aspergillus niger and obtains 2 grades of seed liquors of aspergillus niger.
It is inoculated with for the first time: 3 grades of seed liquors of coronoid process dissipate capsule bacterium is inoculated into the fermentor equipped with agaricus bisporus sea-buckthorn concentrate,
Inoculum concentration 10 (v/v) %；
Aerobic liquid state fermentation: it controls 28 DEG C of agaricus bisporus Leaves of Hippophae L broth temperature in fermentor and is passed through filtrated air, send out
Ferment 48 hours；
Second of inoculation: 2 grades of seed liquors of aspergillus niger are inoculated into the fermentor of the agaricus bisporus sea-buckthorn concentrate, are inoculated with
8.0 (v/v) % are measured, are continued aerobic fermentation 72 hours；
Centrifugation and film filtering: removing mycelium for the strainer that the liquid after fermentation crosses 400 mesh, after butterfly centrifugal machine, from
Scheming revolving speed is 4500 revs/min, obtains more clear centrifugate, then squeezing into membrane aperture is in 0.2 micrometer ceramics film device
Film process is carried out, ceramic membrane permeate is collected, obtains the agaricus bisporus sea-buckthorn tea fermentation liquid of clear.
Agaricus bisporus sea-buckthorn tea fermentation liquid: being carried out that paving disk is quick-frozen and freeze-drying by freeze-drying, obtains agaricus bisporus sea-buckthorn speed
Molten tea.After testing, 13.6 kilograms of agaricus bisporus sea-buckthorn instant teas are made in the present embodiment altogether.Give off a strong fragrance after brewing, flavour is mellow,
Soup look is isabelline, clarification is bright.
Claims (6)
1. a kind of preparation method of agaricus bisporus sea-buckthorn instant tea, which is characterized in that the preparation side of the agaricus bisporus sea-buckthorn instant tea
Method specifically comprises the following steps:
A. water mentions Leaves of Hippophae L and agaricus bisporus: using 75~95 DEG C of water extract Leaves of Hippophae L obtain Leaves of Hippophae L Aqueous extracts, using 80~
90 DEG C of water, which is extracted, obtains agaricus bisporus Aqueous extracts by pretreated agaricus bisporus, filtering, centrifugation；
B. agaricus bisporus sea-buckthorn mixed liquor is concentrated: agaricus bisporus Aqueous extracts and Leaves of Hippophae L Aqueous extracts are mixed, it is mixed to obtain agaricus bisporus sea-buckthorn
Liquid is closed, agaricus bisporus sea-buckthorn mixed liquor is concentrated to the 10%~30% of original volume, obtains agaricus bisporus sea-buckthorn concentrate；
C. it sterilizes: agaricus bisporus sea-buckthorn concentrate being sterilized using UHT method for disinfection；
D. the cultivation of seed liquor: using PDA culture medium activation culture coronoid process dissipate capsule bacterium and aspergillus niger respectively, then mixed with agaricus bisporus sea-buckthorn
It closes liquid to cultivate step by step, cultivates 2 grades of seed liquors of 3 grades of seed liquors of coronoid process dissipate capsule bacterium and aspergillus niger；
E. it is inoculated with for the first time: 3 grades of seed liquors of coronoid process dissipate capsule bacterium is inoculated into agaricus bisporus according to the inoculum concentration of 3%~10% (v/v)
In sea-buckthorn mixed liquor, aerobic 48~120h of liquid state fermentation in the fermenter；
F. it is inoculated with for second: accessing 2 grades of seed liquors of aspergillus niger according to the inoculum concentration of 2%~8% (v/v) in the fermenter, continue
Aerobic 72~144h of liquid state fermentation；
G. centrifugation and film filtering, remove the impurity in fermentation liquid；
H. vacuum drying obtains agaricus bisporus sea-buckthorn instant tea.
2. the preparation method of agaricus bisporus sea-buckthorn instant tea according to claim 1, it is characterised in that: described to use 75~95
DEG C water extracts Leaves of Hippophae L specifically: extracts Leaves of Hippophae L using 75~95 DEG C of softened waters, solid-liquid ratio is 1:10~1:25；When extraction
Between 40-50min, filter to take filtrate, obtain Leaves of Hippophae L Aqueous extracts.
3. the preparation method of agaricus bisporus sea-buckthorn instant tea according to claim 1, it is characterised in that: described to use 80~90
DEG C water extract by pretreated agaricus bisporus and be specially；Using 80~90 DEG C, solid-liquid ratio is that the softened water of 1:10~1:20 mentions
Learn from else's experience pretreated 40~50min of agaricus bisporus, obtains agaricus bisporus Aqueous extracts.
4. the preparation method of agaricus bisporus sea-buckthorn instant tea according to claim 1, it is characterised in that: double in the step B
The mixed proportion of spore mushroom Aqueous extracts and Leaves of Hippophae L Aqueous extracts is between 1:4~1:14.
5. the preparation method of agaricus bisporus sea-buckthorn instant tea according to claim 1, it is characterised in that: the aerobic liquid hair
The temperature of ferment is 28~32 DEG C.
6. the preparation method of agaricus bisporus sea-buckthorn instant tea according to claim 1, which is characterized in that the centrifugation and film mistake
Filter specifically: by 400 mesh filter screen filtrations of the liquid after fermentation, remove mycelium, centrifugation is 0.2 micrometer ceramics with aperture
Permeate is collected by filtration in film, obtains clear agaricus bisporus sea-buckthorn fermentation liquid.
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| RU2045192C1 (en) * | 1991-06-17 | 1995-10-10 | Мелкадзе Реваз Георгиевич | Method for production of tea concentrate |
| WO2004008870A1 (en) * | 2002-07-19 | 2004-01-29 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Isomaltulose-containing instant beverage powder |
| CN101238842A (en) * | 2007-02-05 | 2008-08-13 | 高原圣果沙棘制品有限公司 | Sea-buckthorn instant tea and preparation thereof |
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| CN104585384A (en) * | 2014-12-04 | 2015-05-06 | 赵蔚风 | A production method for seabuckthorn leaf tea with eurotium cristatum |
| CN104886304B (en) * | 2015-04-07 | 2018-01-02 | 安徽农业大学 | A kind of preparation method of high-quality slender joss stick instant dark tea |
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| RU2045192C1 (en) * | 1991-06-17 | 1995-10-10 | Мелкадзе Реваз Георгиевич | Method for production of tea concentrate |
| WO2004008870A1 (en) * | 2002-07-19 | 2004-01-29 | Südzucker Aktiengesellschaft Mannheim/Ochsenfurt | Isomaltulose-containing instant beverage powder |
| CN101238842A (en) * | 2007-02-05 | 2008-08-13 | 高原圣果沙棘制品有限公司 | Sea-buckthorn instant tea and preparation thereof |
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