CN115776987A - 可条码化可交换肽-mhc多聚体文库 - Google Patents

可条码化可交换肽-mhc多聚体文库 Download PDF

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Publication number
CN115776987A
CN115776987A CN202180039282.4A CN202180039282A CN115776987A CN 115776987 A CN115776987 A CN 115776987A CN 202180039282 A CN202180039282 A CN 202180039282A CN 115776987 A CN115776987 A CN 115776987A
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China
Prior art keywords
peptide
mhc
mhci
monomer
multimerization domain
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CN202180039282.4A
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English (en)
Chinese (zh)
Inventor
O·约斯弗森
T·马利亚
A·哈梅尔
J·斯怀恩
B·罗斯寇
M·拉什蒂安
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Ripertoli Immunomedicine Co ltd
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Ripertoli Immunomedicine Co ltd
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Publication of CN115776987A publication Critical patent/CN115776987A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70539—MHC-molecules, e.g. HLA-molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00—Methods specially adapted for identifying library members
    • C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202180039282.4A 2020-03-31 2021-03-31 可条码化可交换肽-mhc多聚体文库 Pending CN115776987A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063003177P 2020-03-31 2020-03-31
US63/003,177 2020-03-31
PCT/US2021/025167 WO2021202727A2 (en) 2020-03-31 2021-03-31 Barcodable exchangeable peptide-mhc multimer libraries

Publications (1)

Publication Number Publication Date
CN115776987A true CN115776987A (zh) 2023-03-10

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CN202180039282.4A Pending CN115776987A (zh) 2020-03-31 2021-03-31 可条码化可交换肽-mhc多聚体文库

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US (1) US20230287394A1 (https=)
EP (1) EP4126923A2 (https=)
JP (1) JP7846628B2 (https=)
CN (1) CN115776987A (https=)
AU (1) AU2021248941A1 (https=)
CA (1) CA3176448A1 (https=)
IL (1) IL296789A (https=)
WO (1) WO2021202727A2 (https=)

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CN114644706B (zh) * 2022-02-16 2024-04-09 国家纳米科学中心 基于DNA纳米技术的pMHC多聚体制备方法及其应用
AU2023314797A1 (en) 2022-07-29 2024-12-12 Repertoire Immune Medicines, Inc. T cell epitopes associated with type 1 diabetes
WO2025072849A1 (en) 2023-09-29 2025-04-03 Repertoire Immune Medicines, Inc. Mhc-presentable peptides associated with type 1 diabetes
WO2025137580A1 (en) * 2023-12-22 2025-06-26 Antiger Therapeutics, Inc. Class ii hla with inter-chain disulfide bond

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CN101111520A (zh) * 2005-02-04 2008-01-23 普罗伊缪因有限公司 Mhc寡聚体及其制备方法
WO2009003493A2 (en) * 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
CN104105503A (zh) * 2012-01-06 2014-10-15 俄勒冈健康科学大学 部分mhc构建体及使用方法
US20170343545A1 (en) * 2014-06-06 2017-11-30 Herlev Hospital Determining Antigen Recognition through Barcoding of MHC Multimers
US20190361010A1 (en) * 2018-02-12 2019-11-28 10X Genomics, Inc. Methods and systems for macromolecule labeling
CN113874384A (zh) * 2019-01-04 2021-12-31 瑞佩尔托利免疫医药股份有限公司 生产高度多样性肽文库和促进蛋白质折叠的方法

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US4657866A (en) 1982-12-21 1987-04-14 Sudhir Kumar Serum-free, synthetic, completely chemically defined tissue culture media
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FR2646437B1 (fr) 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
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WO2009003493A2 (en) * 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
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IL296789A (en) 2022-11-01
EP4126923A2 (en) 2023-02-08
WO2021202727A2 (en) 2021-10-07
JP2023520412A (ja) 2023-05-17
JP7846628B2 (ja) 2026-04-15
CA3176448A1 (en) 2021-10-07
US20230287394A1 (en) 2023-09-14
AU2021248941A1 (en) 2022-11-17
WO2021202727A3 (en) 2021-12-09

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