CN115770486A - Method for comprehensively reducing membrane pollution of membrane bioreactor - Google Patents
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- CN115770486A CN115770486A CN202211575736.2A CN202211575736A CN115770486A CN 115770486 A CN115770486 A CN 115770486A CN 202211575736 A CN202211575736 A CN 202211575736A CN 115770486 A CN115770486 A CN 115770486A
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- 239000012528 membrane Substances 0.000 title claims abstract description 35
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000010791 quenching Methods 0.000 claims abstract description 23
- 230000000171 quenching effect Effects 0.000 claims abstract description 20
- 230000000593 degrading effect Effects 0.000 claims abstract description 10
- 239000010802 sludge Substances 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- 238000009285 membrane fouling Methods 0.000 claims description 9
- 235000013619 trace mineral Nutrition 0.000 claims description 7
- 239000011573 trace mineral Substances 0.000 claims description 7
- JKEJEOJPJVRHMQ-UHFFFAOYSA-N N-octanoyl-L-homoserine lactone Natural products CCCCCCCC(=O)NC1CCOC1=O JKEJEOJPJVRHMQ-UHFFFAOYSA-N 0.000 claims description 6
- 239000002033 PVDF binder Substances 0.000 claims description 6
- JKEJEOJPJVRHMQ-JTQLQIEISA-N n-(2-oxotetrahydrofuran-3-yl)octanamide Chemical compound CCCCCCCC(=O)N[C@H]1CCOC1=O JKEJEOJPJVRHMQ-JTQLQIEISA-N 0.000 claims description 6
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 6
- 239000002351 wastewater Substances 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 4
- 230000003203 everyday effect Effects 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000012510 hollow fiber Substances 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 238000005259 measurement Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000008188 pellet Substances 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims 1
- 239000013028 medium composition Substances 0.000 claims 1
- 238000002791 soaking Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 238000004065 wastewater treatment Methods 0.000 abstract description 3
- 230000001678 irradiating effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000018612 quorum sensing Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 4
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 230000032770 biofilm formation Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012554 master batch record Methods 0.000 description 2
- 230000000116 mitigating effect Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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Abstract
A method for comprehensively reducing membrane pollution of a membrane bioreactor, belonging to the field of wastewater treatment. Adding a population quenching flora capable of degrading signal molecules to the MBR and subjecting the MBR to ultraviolet light. According to the invention, by adding the colony quenching flora capable of degrading AHLs and irradiating with ultraviolet light, the QS effect of microorganisms in MBR is reduced, the formation of a biological membrane on the membrane is slowed down, and further the membrane pollution is relieved.
Description
Technical Field
The invention belongs to the field of wastewater treatment, particularly relates to a membrane bioreactor technology in a wastewater treatment process, and particularly relates to a method and a device for comprehensively reducing membrane pollution of a membrane bioreactor.
Background
A Membrane Bioreactor (MBR) is an activated sludge treatment process combined with membrane filtration, combines biodegradation of activated sludge with efficient separation of membranes, and has the advantages of good solid-liquid separation, good effluent quality, small occupied area and the like. However, membrane fouling severely limits the further spread and application of this technology in practical applications. Membrane fouling is often associated with Quorum Sensing (QS). In quorum sensing processes, bacteria use signal molecules to perform quorum activity, such as the formation of biofilms on membrane surfaces that cause membrane fouling. Therefore, degradation of signal molecules, i.e., quorum Quenching (QQ), is an effective method for controlling membrane fouling. Some scholars have carried out relevant research and put forward corresponding control strategies, such as treatment measures of quenching flora by adding population degrading signal molecules. Exogenously added flora tends to be unstable in the mixed liquor and is susceptible to environmental influences. In addition, under the irradiation of ultraviolet light, nitrate in the solution can generate hydroxyl radicals to degrade signal molecules. Therefore, this study mitigated membrane fouling in MBRs by adding bacterial populations capable of degrading signal molecules in combination with uv irradiation.
Disclosure of Invention
Based on the problems in the prior art, the invention provides a method and a device for improving the MBR performance, and the method improves the sewage treatment performance of the MBR from the group quenching angle. Compared with the prior art, the method can further reduce the membrane pollution problem in the MBR, and has the advantages of simple operation, easy realization and strong practicability.
In order to achieve the purpose, the invention is realized by the following technical scheme:
adding a colony quenching flora capable of degrading signal molecules into the MBR and carrying out ultraviolet illumination on the MBR, wherein the specific operation steps and process conditions are as follows:
(1) Firstly, enriching a colony quenching strain capable of degrading signal molecules; specifically, taking sludge in a stably operated MBR as a sludge sample, and pretreating the sludge by PBS; quenching the flora with a C8-HSL enriched population, adding the pretreated sludge to an Erlenmeyer flask containing 100mL of minimal medium and 20. Mu.M of C8-HSL, and culturing at 30 ℃ and 150rpm for three days; after three days, 10% of the culture broth was taken and cultured in a new medium, which was repeated three times and consisted of: 100mL of minimal medium and 20. Mu.M of C8-HSL; the composition of the minimal medium was as follows: naCl (1 g/L), KCl (0.5 g/L), mgCl 2 (0.4g/L)，CaCl 2 (0.1g/L)，Na 2 SO 4 (0.15g/L)，KH 2 PO 4 (2g/L)，Na 2 HPO 4 (2.25 g/L); finally, the culture solutions were separatedCentrifuge at 8000rpm for 5min. The supernatant was discarded, and the bottom bacterial pellet was added to an Erlenmeyer flask containing 100mL of LB medium and cultured to OD 600 1.0 for subsequent experiments;
(2) Adding the screened flora into an MBR (membrane bioreactor) which operates stably every day, wherein the effective volume of the MBR is 6L, and the volume of the MBR added every day is 10mL; the water in the whole MBR system is always irradiated by an ultraviolet lamp, and the wavelength of the ultraviolet lamp is 320-380nm.
Pumping synthetic wastewater into MBR by a peristaltic pump, wherein COD of the synthetic wastewater is generally 320mg/L, ammonia nitrogen is generally 40mg/L, specifically, the actual measurement is taken as the standard, the rest trace elements are added according to the requirements, and the trace elements I and II are respectively 1mL/L; microelement group I component (g/L): EDTA 5, feSO 4 ·7H 2 O5, trace element II (g/L): EDTA15, cuSO 4 ·5H 2 O 0.2、ZnSO 4 ·7H 2 O 0.43、CoCl 2 ·6H 2 O 0.24、MnCl 2 ·4H 2 O 0.99、Na 2 MoO 4 ·2H 2 O0.22、NiCl 2 ·6H 2 O 0.19、NaSeO 4 0.11、H 3 BO 4 0.014。
MBR (membrane bioreactor) adopts polyvinylidene fluoride (PVDF) hollow fiber membrane (average pore diameter is 0.01um, specific surface area is 0.06 m) 2 ) And is provided with a perforating aeration device. The filtration flow of the MBR is constant and is 15L/(m) 2 H). Extracting the filtrate from the membrane module by a pump, wherein the extraction period is 10 minutes, namely extraction time is 9 minutes, and the rest time is 1 minute; the concentration of suspended solid in the mixed solution is kept between 7000 and 8000mg/L. The sludge retention time was 30 days. The reactor temperature was maintained at about 24 ℃ and the pH was maintained at about 7.3. The wavelength of the ultraviolet lamp is 320-380nm. A pressure sensor is installed to detect the transmembrane pressure TMP. When the TMP value increased to 40kpa, the membrane module was taken out and washed (tap water rinse, 1% naclo solution wash, 10% citric acid soak for 8 hours).
The invention has the following function principle: the attachment and growth of bacteria on the membrane surface results in the formation of a biofilm, which is a major cause of membrane fouling. Biofilm formation is generally attributed to a phenomenon known as Quorum Sensing (QS). In the QS process, bacteria perform various types of quorum behavior, including biofilm formation, by secreting signal molecules. Degradation of signal molecules, i.e., quorum Quenching (QQ), is an effective method for mitigating membrane fouling. The quorum-quenching bacteria degrade the signal molecules by secreting quorum-quenching enzymes. Under the irradiation of ultraviolet light, nitrate in the solution can generate hydroxyl radicals to degrade signal molecules. Therefore, the QS effect of microorganisms in the MBR is reduced by adding the colony quenching flora capable of degrading AHLs and irradiating with ultraviolet light, the formation of a biofilm on the membrane is slowed down, and the membrane pollution is further relieved.
Compared with the prior art, the invention has the following advantages and effects:
(1) Meanwhile, degrading signal molecules by using colony quenching flora and ultraviolet light.
(2) The operation process is simple and easy to realize.
Drawings
Fig. 1 shows the filtration cycle of each reactor (1 square corresponds to one filtration cycle followed by washing, the longer the square corresponds to, the less contamination), R1: contrasting MBR; r2: adding MBR of colony quenching flora; r3: MBR of ultraviolet illumination; r4: MBR for quenching flora and ultraviolet light is added.
Fig. 2 shows a corresponding experimental setup.
1, a water inlet tank; 2, a peristaltic pump; 3, a membrane component; 4, an aeration device; 5 a pressure sensor; 6 ultraviolet lamp.
Detailed Description
In accordance with the above technical solutions, the following detailed descriptions of the present invention are provided with reference to the accompanying drawings and examples, it should be noted that the present invention is not limited to the following specific embodiments, and all equivalent changes based on the technical solutions of the present application fall within the protection scope of the present invention. The present invention will be described in further detail with reference to examples.
Example 1:
effect of colony quenching on MBR membrane contamination
Four MBR reactors are arranged, namely a control MBR (R1), an MBR (R2) for adding colony quenching flora, an MBR (R3) for ultraviolet irradiation, and an MBR (R4) for simultaneously adding colony quenching flora and performing ultraviolet irradiation.
Four MBRs run in parallel, and the effective volume of MBR is 6L. The synthetic wastewater is pumped into the MBR by a peristaltic pump. Theoretical amount of main nutrients of the synthetic wastewater: COD is 320mg/L, ammonia nitrogen is 40mg/L, and the rest is added according to the requirements based on actual measurement. The trace elements I and II are respectively 1mL/L. Microelement group I component (g/L): EDTA 5, feSO 4 ·7H 2 And (3) O5. And the microelement II comprises the following components (g/L): EDTA15, cuSO 4 ·5H 2 O 0.2、ZnSO 4 ·7H 2 O 0.43、CoCl 2 ·6H 2 O 0.24、MnCl 2 ·4H 2 O 0.99、Na 2 MoO 4 ·2H 2 O 0.22、NiCl 2 ·6H 2 O0.19、NaSeO 4 0.11、H 3 BO 4 0.014. The MBR adopts a polyvinylidene fluoride (PVDF) hollow fiber membrane (average pore diameter is 0.01um, specific surface area is 0.06 m) 2 ) And is equipped with a perforating aeration device. The filtration flow of the MBR is constant and is 15L/(m) 2 H). The filtrate was extracted from the membrane module with a pump for 10 minutes (9 minutes extraction, 1 minute rest). The wavelength of the ultraviolet lamp is 320-380nm. The concentration of suspended solid in the mixed solution is kept between 7000 and 8000mg/L. The sludge retention time was 30 days. The reactor temperature was maintained at about 24 ℃ and the pH at about 7.3. A pressure sensor is installed to detect transmembrane pressure (TMP). When the TMP value increased to 40kpa, the membrane module was taken out and washed (tap water rinse, 1% naclo solution wash, 10% citric acid soak for 8 hours).
Results and analysis
Example 1
As shown in FIG. 1, the average filtration period for R1 was 3.50 days, the average filtration period for R2 was 5.49 days, the average filtration period for R3 was 5.51 days, and the average filtration period for R4 was 8.34 days. Therefore, the MBR can be effectively prolonged by adding colony quenching flora or ultraviolet irradiation, and the effect is similar. Importantly, the average filtration period of R4 is much higher than that of R2 and R3, which indicates that the simultaneous addition of the quorum-quenching flora and the illumination of MBR with ultraviolet light has the most significant effect on the mitigation of membrane fouling.
Claims (3)
1. A method for comprehensively reducing membrane pollution of a membrane bioreactor is characterized in that a colony quenching flora capable of degrading signal molecules is added into an MBR and the MBR is subjected to ultraviolet illumination, and the specific operation steps and process conditions are as follows:
(1) Firstly, enriching a colony quenching strain capable of degrading signal molecules; specifically, taking sludge in a MBR which is operated stably as a sludge sample, and pretreating the sludge by using PBS; quenching the flora with a C8-HSL enriched population, adding the pretreated sludge to an Erlenmeyer flask containing 100mL of minimal medium and 20. Mu.M of C8-HSL, and culturing at 30 ℃ and 150rpm for three days; after three days, 10% of the culture broth was added to a new medium and the process was repeated three times, the new medium composition: 100mL of minimal medium and 20. Mu.M of C8-HSL; the composition of the minimal medium is as follows: naCl (1 g/L), KCl (0.5 g/L), mgCl 2 (0.4g/L)，CaCl 2 (0.1g/L)，Na 2 SO 4 (0.15g/L)，KH 2 PO 4 (2g/L)，Na 2 HPO 4 (2.25 g/L); finally, the culture solution was centrifuged at 8000rpm for 5min, respectively; the supernatant was discarded, and the bottom bacterial pellet was added to an Erlenmeyer flask containing 100mL of LB medium and cultured to OD 600 1.0 for subsequent experiments;
(2) Adding the screened flora into a stable running MBR every day, wherein the effective volume of the MBR is 6L, and the volume added every day is 10mL; the water in the whole MBR system is always irradiated by an ultraviolet lamp, and the wavelength of the ultraviolet lamp is 320-380nm.
2. The method for comprehensively alleviating membrane fouling of a membrane bioreactor according to claim 1, wherein in the step (2), the synthetic wastewater is pumped into the MBR by a peristaltic pump, the COD of the synthetic wastewater is generally 320mg/L, the ammonia nitrogen is generally 40mg/L, specifically, based on actual measurement, the rest trace elements are added according to the requirement, and the trace elements I and II are 1mL/L respectively; microelement group I component (g/L): EDTA 5, feSO 4 ·7H 2 O5, trace element II (g/L): EDTA15, cuSO 4 ·5H 2 O 0.2、ZnSO 4 ·7H 2 O 0.43、CoCl 2 ·6H 2 O 0.24、MnCl 2 ·4H 2 O0.99、Na 2 MoO 4 ·2H 2 O 0.22、NiCl 2 ·6H 2 O 0.19、NaSeO 4 0.11、H 3 BO 4 0.014。
3. The method of claim 1, wherein the MBR is made of polyvinylidene fluoride (PVDF) hollow fiber membrane and is equipped with a perforated aeration device, and the filtering flow rate of the MBR is constant at 15L/(m) 2 H), extracting the filtrate from the membrane module with a pump for 10 minutes, i.e. 9 minutes of extraction and 1 minute of rest; the mixed liquor suspended solids concentration was maintained at 7000-8000mg/L, the sludge retention time was 30 days, the reactor temperature was maintained at 24 ℃ and the pH was maintained at 7.3, a pressure sensor was installed to detect the transmembrane pressure TMP, and when the TMP value rose to 40kpa, the membrane module was taken out for cleaning, which was flushed with tap water, 1% NaCl solution washing, 10% citric acid soaking for 8 hours.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296678A (en) * | 2015-11-26 | 2016-02-03 | 江苏大学 | Biomembrane control method based on photocatalytic quenching quorum sensing signal |
| CN107158957A (en) * | 2017-03-22 | 2017-09-15 | 北京大学深圳研究生院 | The method that immobilization bacterial strain controls fouling membrane is quenched in a kind of utilization quorum sensing |
| KR20200112467A (en) * | 2019-03-22 | 2020-10-05 | 경북대학교 산학협력단 | Method for microbial quorum quenching by light irradiation and method for controlling biological pollution |
| CN112939139A (en) * | 2021-03-04 | 2021-06-11 | 汕头大学 | Photocatalysis membrane reactor and sewage treatment system |
| CN114455697A (en) * | 2022-03-16 | 2022-05-10 | 苏州盛虹环保科技有限公司 | MBR membrane pollution control method based on microbial population induction quenching |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296678A (en) * | 2015-11-26 | 2016-02-03 | 江苏大学 | Biomembrane control method based on photocatalytic quenching quorum sensing signal |
| CN107158957A (en) * | 2017-03-22 | 2017-09-15 | 北京大学深圳研究生院 | The method that immobilization bacterial strain controls fouling membrane is quenched in a kind of utilization quorum sensing |
| KR20200112467A (en) * | 2019-03-22 | 2020-10-05 | 경북대학교 산학협력단 | Method for microbial quorum quenching by light irradiation and method for controlling biological pollution |
| CN112939139A (en) * | 2021-03-04 | 2021-06-11 | 汕头大学 | Photocatalysis membrane reactor and sewage treatment system |
| CN114455697A (en) * | 2022-03-16 | 2022-05-10 | 苏州盛虹环保科技有限公司 | MBR membrane pollution control method based on microbial population induction quenching |
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