CN114455697A - MBR membrane pollution control method based on microbial population induction quenching - Google Patents
MBR membrane pollution control method based on microbial population induction quenching Download PDFInfo
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- 239000012528 membrane Substances 0.000 title claims abstract description 64
- 230000000813 microbial effect Effects 0.000 title claims abstract description 16
- 238000010791 quenching Methods 0.000 title claims abstract description 16
- 230000000171 quenching effect Effects 0.000 title claims abstract description 16
- 238000011954 pollution control method Methods 0.000 title claims abstract description 15
- 230000006698 induction Effects 0.000 title abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 241000894006 Bacteria Species 0.000 claims abstract description 25
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical class OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000010802 sludge Substances 0.000 claims abstract description 18
- 239000012266 salt solution Substances 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 11
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 7
- 239000011259 mixed solution Substances 0.000 claims abstract description 7
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 37
- 230000018612 quorum sensing Effects 0.000 claims description 20
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 239000010865 sewage Substances 0.000 claims description 16
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000005406 washing Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 claims description 7
- 239000012510 hollow fiber Substances 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 239000007836 KH2PO4 Substances 0.000 claims description 6
- 108091006629 SLC13A2 Proteins 0.000 claims description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 230000015556 catabolic process Effects 0.000 claims description 6
- 238000006731 degradation reaction Methods 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 230000002572 peristaltic effect Effects 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000011550 stock solution Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 239000008363 phosphate buffer Substances 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 238000001556 precipitation Methods 0.000 claims description 4
- 239000002033 PVDF binder Substances 0.000 claims description 3
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 238000013480 data collection Methods 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 239000003344 environmental pollutant Substances 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 231100000719 pollutant Toxicity 0.000 claims description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 239000008055 phosphate buffer solution Substances 0.000 claims 2
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims 1
- 229910000397 disodium phosphate Inorganic materials 0.000 claims 1
- 239000013618 particulate matter Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 4
- 239000007983 Tris buffer Substances 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 1
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- 239000008223 sterile water Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/006—Regulation methods for biological treatment
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/08—Prevention of membrane fouling or of concentration polarisation
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Abstract
The invention discloses an MBR membrane pollution control method based on microbial population induction quenching, which comprises the following steps: s1: reaction using MBR reactor, S2: inoculating activated sludge at 1% v/v into 20mL of M9 medium with GCL as a single carbon source, culturing for 5-7 days at 30 ℃ in a shaker at 150rpm, S3: the method comprises the steps of cleaning GCL enriched bacteria or activated sludge with TRIS buffer salt solution with pH being 7.0 of 50mmol/L, adding 100uL of pure water into 400nmol/L AHL with the same volume as the GCL enriched bacteria or the activated sludge to serve as blank control, boiling the activated sludge for 10min, adjusting OD600 to be 1.0, culturing the mixed solution in a shaking table with the temperature being 30 ℃ and the rpm being 120rpm, taking out one part of the mixed solution regularly, placing the mixed solution in water bath with the temperature being 99 ℃ for 10min to inactivate bacteria, and completing pollution control of the MBR membrane.
Description
Technical Field
The invention relates to the field of pollution control of MBR (membrane bioreactor) membranes, in particular to an MBR membrane pollution control method based on microbial quorum induction quenching.
Background
The technology of the membrane bioreactor has been traced from maturity to popularization and application, but the problem of membrane pollution is difficult to solve and hinders the development of the technology, in the field of microbial research, the concept of microbial quorum sensing is almost proposed at the same time as the technology of the membrane bioreactor, and the technology is rapidly developed, the microbial quorum sensing, namely bacteria sense the density of a flora by secreting signal molecules and detecting the concentration of the signal molecules, so as to trigger a series of group behaviors, previously, the research of quorum sensing is mainly concentrated in the field of microbes, in recent years, some reports about quorum sensing in the water treatment technology are also appeared in succession, and the research obtains good membrane pollution control effect by inhibiting the microbial quorum sensing in the MBR, which provides a new thought for membrane pollution control, but the research about quorum sensing and quenching in the membrane bioreactor is still in the exploration stage, the influence of the operating parameters of the membrane bioreactor, such as sludge retention time, on quorum sensing in the reactor has not been thoroughly discussed, the reported quorum sensing quenching measures are too complex and the stability of the quorum sensing quenching measures is yet to be enhanced, and the influence of the quorum sensing quenching-based membrane pollution control measures on the pollution removal efficiency of the MBR has not been carefully examined.
Disclosure of Invention
The invention mainly aims to provide an MBR membrane pollution control method based on microbial quorum sensing quenching, which can effectively solve the problems in the background technology.
In order to achieve the purpose, the invention adopts the technical scheme that:
a MBR membrane pollution control method based on microbial quorum sensing quenching comprises the following operation steps:
s1: preparing materials: hollow fiber membrane, domestic sewage, activated sludge, M9 culture medium, Tris buffer salt solution and phosphate buffer salt solution;
s2: the reaction was carried out using an MBR reactor: when the device is used, the temperature needs to be guaranteed to be about 20-26 ℃, domestic sewage filtered by a 1mm screen is added into a high-level water tank every day, raw water in the high-level water tank enters each constant-water-level water tank and is redistributed into a reactor, then water is pumped out by a peristaltic pump, a pressure transmitter is connected between a membrane module and the pumping peristaltic pump, transmembrane pressure difference data in each MBR are accurately measured and collected, a single chip microcomputer collects and processes signals of the pressure transmitter and transmits the signals to a computer to record data, the data collection frequency is 1 minute/time, after the membrane module in the MBR is completely polluted, when the transmembrane pressure difference is greater than 70kPa, the membrane module is taken out, pollutants on the surface of the membrane are washed off by tap water, then a chemical cleaning solution containing 0.01mol L of NaOH and 200ppm of sodium hypochlorite is used for soaking for 1-2 hours, and the membrane module can be reused after chemical cleaning;
s3: inoculating the activated sludge into 20mL of M9 culture medium which takes GCL as a single carbon source at 1% v/v, culturing for 5-7 days in a shaking table at the temperature of 30 ℃ and at the speed of 150rpm, transferring the bacterium liquid into a new M9 culture medium which takes GCL as a single carbon source after the bacterium liquid becomes turbid, continuously culturing with the culture medium of 20mL, completing enrichment after three transfer cultures, and storing the obtained bacteria in a refrigerator at the temperature of 4 ℃;
s4: washing GCL enriched bacteria or activated sludge with 50mmol/L Tris buffer salt solution with pH 7.0, centrifuging at 7000rpm for 10min with a centrifuge washing machine at 4 ℃, discarding supernatant, adding TRIS buffer salt, washing twice, adjusting the concentration of the bacteria solution to 1.0 at 600nm with TRIS buffer salt, diluting 1g/L AHL stock solution to 400nmol/L with TRIS buffer salt, adding 100uL of above bacteria solution and equal-volume 400nmol/L AHL solution into 1.5mL centrifuge tube, repeatedly preparing several parts for continuous sampling in subsequent degradation experiments, adding 100uL of pure water into equal-volume 400nmol/L AHL as blank control, boiling activated sludge for 10min, adjusting OD600 to 1.0, culturing the mixed solution in a shaking table at 30 ℃ and 120rpm, taking out one part at regular time, placing in 99 ℃ for 10min, inactivating bacteria in water bath, after bacteria are filtered out by a filter membrane of 0.45um, the sample is stored at 4 ℃, and then the pollution control of the MBR membrane can be finished.
Preferably, its material of hollow fiber membrane that adopts in the experiment is polyvinylidene fluoride, and the aperture is 0.01um, and the membrane silk external diameter is 1.85mm, and single membrane silk length is 60cm, use up within two days after the domestic sewage water intaking, make the messengerBefore use, the particles in the sewage are filtered by a sieve with the diameter of 1mm, and then 255mg/L NaHCO is added into the water3To adjust the alkalinity.
Preferably, the M9 medium is prepared by preparing 5XM9 salt and mixing 92.39g of Na2HPO4-12H2O, 15g KH2PO42.5g of NaC1 and 5.0g of NH4Dissolving Cl in 800mL of distilled water, fully dissolving, diluting to 1000mL, adjusting pH to 7.4 with prepared NaOH solution, sterilizing at 121 deg.C for 20min, storing at room temperature, collecting about 700mL of sterilized water, adding 200mL of prepared and sterilized 5XM9 salt, adding 2mL of sterilized 1mol L MgSO (MgSO) containing sterilized 1mol L of MgSO 94The solution was added 2mLGCL and 100uL of sterilized 1mol/LCaCl2If CaCl is added2When the solution is still hot, precipitation can appear, at the moment, the solution is vigorously shaken until the precipitation is dissolved, sterilized distilled water is used for fixing the volume to 1000mL, and the solution is stored at normal temperature for use.
Preferably, the Tris buffer solution is prepared by dissolving 6.05g of Tris and 8.76g of NaCl in 800mL of deionized water, adjusting the pH to 7.0 with 1mol of HC1 solution, dissolving in 1L, sterilizing at 121 ℃ for 20min, and storing at normal temperature for later use.
Preferably, the phosphate buffered saline solution is prepared by firstly preparing 10x stock solution of phosphate buffered saline solution, 80g of NaC1, 2g of KCl and 35.8g of Na2HPO4-12H2O, 2.4g KH2PO4Dissolving in 800mL of water, fully dissolving, fixing the volume to 1000mL, adjusting the pH value to 7.4, and storing at normal temperature.
Compared with the prior art, the invention has the following beneficial effects:
in the present invention, by adding NaHCO3Can adjust the basicity in the domestic sewage, because ammonia nitrogen concentration is higher in the domestic sewage, avoid the digestion in MBR can excessively consume the basicity for pH in the reactor reduces, after reacting again, makes the cell of adhesion in its membrane surface biomembrane and divide secreted polysaccharide material to reduce, proves along with the increase of mud dwell time, and the biological pollution in the MBR is lighter, meets in the mud crowd again and gathers thingsLong-chain signal molecules secreted by the somatic sensing bacteria are more easily degraded by colony sensing rough sterilization, the concentration of the long chain in the reactor is higher until the long chain is completely degraded, the degradation performance of the sludge on the signal molecules is better under the method, and the concentration of the signal molecules in the reactor is correspondingly lower.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention relates to an MBR membrane pollution control method based on microbial quorum sensing quenching, which comprises the following steps:
s1: preparing materials: the central fiber membrane, the domestic sewage, the activated sludge, the M9 culture medium, the Tris buffer salt solution and the phosphate buffer salt solution are adopted in the experiment, the hollow fiber membrane adopted in the experiment is made of polyvinylidene fluoride, the aperture is 0.01um, the outer diameter of the membrane wire is 1.85mm, the length of a single membrane wire is 60cm, the hollow fiber membrane is used up within two days after the domestic sewage is taken, before the hollow fiber membrane is used, a sieve with the diameter of 1mm is firstly used for filtering particles in the sewage, and then NaHCO with the volume of 255mg/L is added into the water3When preparing the M9 culture medium for adjusting alkalinity, firstly preparing 5XM9 salt and mixing 92.39g of Na2HPO4-12H2O, 15g KH2PO42.5g of NaC1 and 5.0g of NH4Dissolving Cl in 800mL of distilled water, fully dissolving, diluting to 1000mL, adjusting pH to 7.4 with a prepared NaOH solution, sterilizing at 121 deg.C for 20min, storing at room temperature, collecting about 700mL of sterilized water, adding 200mL of prepared and sterilized 5xM9 salt, adding 2mL of sterilized 1mol L MgSO (MgSO) containing sterile water4The solution was added 2mLGCL and 100uL of sterilized 1mol/LCaCl2If CaCl is added2The solution is still hot and precipitates, then the solution is vigorously shaken until the precipitates are dissolved, sterilized distilled water is used for fixing the volume to 1000mL, and the solution is stored at normal temperature for useWhen preparing TRIS buffer salt solution, 6.05g of TRIS (hydroxymethyl) aminomethane and 8.76g of NaCl are dissolved in 800mL of deionized water, the pH value of the solution is adjusted to 7.0 by using 1mol L of HC1 solution, the solution is dissolved in 1L, the solution is sterilized at 121 ℃ for 20min and then stored at normal temperature for use, when preparing phosphate buffer salt solution, 10x phosphate buffer salt solution stock solution is prepared, 80g of NaC1, 2g of KCI and 35.8g of Na are prepared2HPO4-12H2O, 2.4g KH2PO4Dissolving in 800mL of water, fully dissolving, fixing the volume to 1000mL, adjusting the pH value to 7.4, and storing at normal temperature.
S2: the reaction was carried out using an MBR reactor: when the device is used, the temperature needs to be guaranteed to be about 20-26 ℃, domestic sewage filtered by a 1mm screen is added into a high-level water tank every day, raw water in the high-level water tank enters each constant-water-level water tank and is redistributed into a reactor, then water is pumped out by a peristaltic pump, a pressure transmitter is connected between a membrane module and a pumping peristaltic pump, transmembrane pressure difference data in each MBR are accurately measured and collected, a single chip microcomputer collects and processes signals of the pressure transmitter and transmits the signals to a computer to record data, the data collection frequency is 1 minute/time, after membrane modules in the MBRs are completely polluted, when the transmembrane pressure difference is greater than 70kPa, the membrane modules are taken out, pollutants on the surface of the membranes are washed off by tap water, then chemical cleaning liquid containing 0.01mol L of NaOH and 200ppm of sodium hypochlorite is used for soaking for 1-2 hours, and the membrane modules can be reused after chemical cleaning.
S3: inoculating the activated sludge into 20mL of M9 culture medium which takes GCL as a single carbon source at 1% v/v, culturing for 5-7 days in a shaking table at 150rpm at 30 ℃, transferring the turbid bacterial liquid into a new M9 culture medium which takes GCL as a single carbon source at 20mL after the bacterial liquid turns turbid, continuing culturing, completing enrichment after three transfer cultures, and storing the obtained bacteria in a refrigerator at 4 ℃.
S4: washing GCL enriched bacteria or activated sludge with TRIS buffer salt solution with concentration of 50mmol/LpH ═ 7.0, centrifuging at 7000rpm for 10min at 4 ℃, discarding supernatant, adding Tris buffer salt for washing twice, adjusting the concentration of bacteria liquid to absorbance of 1.0 at 600nm with Tris buffer salt, diluting AHL stock solution with 1g/L to 400nmol with TRIS buffer salt, adding 100uL of above bacteria liquid and 400nmol of equal volume of AHL solution into 1.5mL centrifuge tube, repeatedly preparing several parts for continuous sampling in subsequent degradation experiment, adding 100uL of pure water into 400nmol of equal volume of AHL as blank control, boiling activated sludge for 10min, adjusting OD600 ═ 1.0, culturing the mixed solution in 30 ℃, 120rpm shaking table, taking out one part at regular time, placing in 99 ℃ mixed solution for 10min, inactivating bacteria, filtering the sample in water bath with 0.45um filter membrane at 4 ℃, at this time, the pollution control of the MBR membrane can be completed.
The invention is realized by adding NaHCO3The alkalinity in the domestic sewage can be adjusted, because the ammonia nitrogen concentration in the domestic sewage is higher, avoid the digestion in MBR can excessively consume the alkalinity, make pH in the reactor reduce, after reacting again, make the cell of adhesion in its membrane surface biomembrane and divide secreted polysaccharide material to reduce, prove along with the increase of mud dwell time, the biological pollution in MBR is lighter, meet the long chain signal molecule that quorum sensing bacterium secretes in the mud more easily by quorum sensing coarse sterilization degradation, then the concentration of long chain can be higher in the reactor, until being degraded completely, the degradation performance of mud to signal molecule is better and the concentration of signal molecule in the reactor is also corresponding lower under this method.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A MBR membrane pollution control method based on microbial quorum sensing quenching is characterized in that: the method comprises the following operation steps:
s1: preparing materials: hollow fiber membrane, domestic sewage, activated sludge, M9 culture medium, Tris buffer salt solution and phosphate buffer salt solution;
s2: the reaction was carried out using an MBR reactor: when in use, the temperature is required to be about 20-26 ℃, the domestic sewage filtered by the 1mm screen is added into a high-level water tank every day, raw water in a high-level water tank enters each constant-water-level water tank and is redistributed to a reactor, then water is pumped out by a peristaltic pump, a pressure transmitter is connected between a membrane component and the pumping peristaltic pump, transmembrane pressure difference data in each MBR are accurately measured and collected, a single chip microcomputer collects and processes signals of the pressure transmitter and transmits the signals to a computer to record data, the data collection frequency is 1 minute/time, after membrane components in the MBR are completely polluted, when the transmembrane pressure difference is greater than 70kPa, taking out the membrane, firstly washing off pollutants on the surface of the membrane by using tap water, then soaking the membrane for 1-2 hours by using chemical cleaning solution containing 0.01mol/L NaOH and 200ppm sodium hypochlorite, and repeatedly using the membrane after chemical cleaning;
s3: inoculating 1% v/v of activated sludge into 20mL of M9 culture medium which takes GCL as a single carbon source, culturing for 5-7 days in a shaking table at 150rpm at 30 ℃, transferring the turbid liquid into a new M9 culture medium which takes GCL as a single carbon source and takes 20mL of culture medium, continuously culturing, completing enrichment after three transfer cultures, and storing the obtained bacteria in a refrigerator at 4 ℃;
s4: washing GCL enriched bacteria or activated sludge with 50mmol/L Tris buffer salt solution with pH 7.0, centrifuging at 7000rpm for 10min with a centrifuge washing machine at 4 ℃, discarding supernatant, adding TRIS buffer salt, washing twice, adjusting the concentration of the bacteria solution to 1.0 at 600nm with TRIS buffer salt, diluting 1g/L AHL stock solution to 400nmol/L with TRIS buffer salt, adding 100uL of above bacteria solution and equal-volume 400nmol/L AHL solution into 1.5mL centrifuge tube, repeatedly preparing several parts for continuous sampling in subsequent degradation experiments, adding 100uL of pure water into equal-volume 400nmol/L AHL as blank control, boiling activated sludge for 10min, adjusting OD600 to 1.0, culturing the mixed solution in a shaking table at 30 ℃ and 120rpm, taking out one part at regular time, placing in 99 ℃ for 10min, inactivating bacteria in water bath, after bacteria are filtered out by a filter membrane of 0.45um, the sample is stored at 4 ℃, and then the pollution control of the MBR membrane can be finished.
2. The MBR membrane pollution control method based on microbial quorum sensing quenching of claim 1The method is characterized in that: its material of hollow fiber membrane that adopts in the experiment is polyvinylidene fluoride, and the aperture is 0.01um, and the membrane silk external diameter is 1.85mm, and single membrane silk length is 60cm, use up in two days after the domestic sewage water intaking, before using, filter the particulate matter in the sewage with 1 mm's sieve earlier, add 255 mg/L's NaHCO to the aquatic again3To adjust the alkalinity.
3. The MBR membrane pollution control method based on microbial quorum sensing quenching according to claim 1, wherein the MBR membrane pollution control method comprises the following steps: when the M9 culture medium is prepared, firstly 5XM9 salt is prepared, and 92.39g of Na is added2HPO4-12H2O, 15g KH2PO42.5g of NaC1 and 5.0g of NH4Dissolving Cl in 800mL of distilled water, fully dissolving, diluting to 1000mL, adjusting pH to 7.4 with prepared NaOH solution, sterilizing at 121 deg.C for 20min, storing at room temperature, collecting about 700mL of sterilized water, adding 200mL of prepared and sterilized 5XM9 salt, adding 2mL of sterilized 1mol/L MgSO (MgSO) of MgSO 1mol/L4The solution was added 2mL GCL and 100uL of sterilized 1mol/LCaCl2If CaCl is added2When the solution is still hot, precipitation can appear, at the moment, the solution is vigorously shaken until the precipitation is dissolved, sterilized distilled water is used for fixing the volume to 1000mL, and the solution is stored at normal temperature for use.
4. The MBR membrane pollution control method based on microbial quorum sensing quenching according to claim 1, wherein the MBR membrane pollution control method comprises the following steps: when the Tris buffer salt solution is prepared, 6.05g of Tris (hydroxymethyl) aminomethane and 8.76g of NaCl are dissolved in 800mL of deionized water, the pH value of the solution is adjusted to 7.0 by using 1mol/L HC1 solution, the solution is dissolved in 1L, and the solution is stored at normal temperature after being sterilized at 121 ℃ for 20min for later use.
5. The MBR membrane pollution control method based on microbial quorum sensing quenching according to claim 1, wherein the MBR membrane pollution control method comprises the following steps: when preparing the phosphate buffer solution, firstly preparing 10x phosphate buffer solution stock solution, and mixing 80g of NaC1, 2g of KCI and 35.8g of Na2HPO4·12H2O, 2.4g KH2PO4Dissolving in 800mL water, fully dissolving, fixing the volume in 1000mL, adjusting the pH value to 7.4, and storing at normal temperature.
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