CN115770486B - Method for comprehensively reducing membrane pollution of membrane bioreactor - Google Patents
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- CN115770486B CN115770486B CN202211575736.2A CN202211575736A CN115770486B CN 115770486 B CN115770486 B CN 115770486B CN 202211575736 A CN202211575736 A CN 202211575736A CN 115770486 B CN115770486 B CN 115770486B
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- 239000012528 membrane Substances 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000010791 quenching Methods 0.000 claims abstract description 20
- 230000000171 quenching effect Effects 0.000 claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 10
- 230000000593 degrading effect Effects 0.000 claims abstract description 10
- 239000010802 sludge Substances 0.000 claims description 13
- 238000001914 filtration Methods 0.000 claims description 11
- 239000000243 solution Substances 0.000 claims description 11
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- JKEJEOJPJVRHMQ-UHFFFAOYSA-N N-octanoyl-L-homoserine lactone Natural products CCCCCCCC(=O)NC1CCOC1=O JKEJEOJPJVRHMQ-UHFFFAOYSA-N 0.000 claims description 6
- JKEJEOJPJVRHMQ-JTQLQIEISA-N n-(2-oxotetrahydrofuran-3-yl)octanamide Chemical compound CCCCCCCC(=O)N[C@H]1CCOC1=O JKEJEOJPJVRHMQ-JTQLQIEISA-N 0.000 claims description 6
- 239000002351 wastewater Substances 0.000 claims description 6
- 239000002033 PVDF binder Substances 0.000 claims description 5
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 5
- 238000005273 aeration Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 239000012510 hollow fiber Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 235000013619 trace mineral Nutrition 0.000 claims description 3
- 239000011573 trace mineral Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000002474 experimental method Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 239000013028 medium composition Substances 0.000 claims description 2
- 239000008188 pellet Substances 0.000 claims description 2
- 238000005086 pumping Methods 0.000 claims description 2
- 239000006228 supernatant Substances 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000004065 wastewater treatment Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 2
- 238000009285 membrane fouling Methods 0.000 description 7
- 230000018612 quorum sensing Effects 0.000 description 7
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 229910002651 NO3 Inorganic materials 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 230000032770 biofilm formation Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 2
- 230000014759 maintenance of location Effects 0.000 description 2
- 238000012554 master batch record Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011217 control strategy Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Separation Using Semi-Permeable Membranes (AREA)
Abstract
A method for comprehensively reducing membrane pollution of a membrane bioreactor belongs to the field of wastewater treatment. Adding a population-quenched population capable of degrading the signal molecules to the MBR and subjecting the MBR to ultraviolet light. According to the invention, the group quenching bacteria capable of degrading AHLs are added, and the QS effect of microorganisms in MBR is lightened by ultraviolet irradiation, so that the formation of biological films on the films is slowed down, and further the film pollution is relieved.
Description
Technical Field
The invention belongs to the field of wastewater treatment, in particular relates to a membrane bioreactor technology in the wastewater treatment process, and particularly relates to a method and a device for comprehensively reducing membrane pollution of a membrane bioreactor.
Background
The Membrane Bioreactor (MBR) is an activated sludge treatment process combined with membrane filtration, combines the biodegradation of activated sludge with the efficient separation of the membrane, and has the advantages of good solid-liquid separation, good effluent quality, small occupied area and the like. However, membrane fouling severely limits further popularization and application of this technology in practical applications. Membrane fouling is often associated with Quorum Sensing (QS). During quorum sensing, bacteria use signal molecules to perform quorum events, such as the formation of biofilm on the membrane surface that causes membrane fouling. Thus, by degrading the signal molecule, namely, population quenching (QQ), is an effective method of controlling membrane fouling. Some scholars have conducted related studies and proposed corresponding control strategies, such as treatment measures of population quenching of the population by adding degradation signal molecules. Exogenously added flora tends to be unstable in the mixed liquor and is susceptible to environmental influences. In addition, under the irradiation of ultraviolet light, nitrate in the solution can generate hydroxyl radical degradation signal molecules. Thus, the present study reduces membrane fouling in MBRs by adding a flora capable of degrading signal molecules in combination with uv light irradiation.
Disclosure of Invention
Based on the problems in the prior art, the invention provides a method and a device for improving the performance of MBR. Compared with the prior art, the method can further alleviate the membrane pollution problem in MBR, is simple to operate, easy to realize and high in practicability.
In order to achieve the above purpose, the present invention is realized by the following technical scheme:
adding a population quenching bacteria group capable of degrading signal molecules into the MBR and carrying out ultraviolet irradiation on the MBR, wherein the specific operation steps and process conditions are as follows:
(1) First enriching a population quenching strain capable of degrading signal molecules; specifically, sludge in the MBR which is operated stably is taken as a sludge sample, and PBS is used for pretreatment of the sludge; quenching the population with a concentrated population of C8-HSL, adding the pretreated sludge into a conical flask containing 100mL of minimal medium and 20. Mu.M of C8-HSL, and culturing at 30℃and 150rpm for three days; after three days, 10% of the culture solution was taken and cultured in a new medium, and the procedure was repeated three times, with a new medium composition: 100mL minimal medium and 20. Mu.M C8-HSL; the composition of the minimal medium was as follows: naCl (1 g/L), KCl (0.5 g/L), mgCl 2 (0.4g/L),CaCl 2 (0.1g/L),Na 2 SO 4 (0.15g/L),KH 2 PO 4 (2g/L),Na 2 HPO 4 (2.25 g/L); finally, the culture solutions were centrifuged at 8000rpm for 5min, respectively. The supernatant was discarded, the bottom bacterial pellet was added to a conical flask containing 100mL LB medium and incubated to OD 600 1.0 for subsequent experiments;
(2) Then adding the selected flora into a stably-running MBR (membrane bioreactor) every day, wherein the effective volume of the MBR is 6L, and the added volume is 10mL every day; the water in the whole MBR system is always irradiated by an ultraviolet lamp, and the wavelength of the ultraviolet lamp is 320-380nm.
Pumping the synthetic wastewater into MBR by a peristaltic pump, wherein the COD of the synthetic wastewater is generally 320mg/L, the ammonia nitrogen is generally 40mg/L, the actual measurement is taken as a standard, the other microelements are added as required, and the microelements I and II are respectively 1mL/L; microelement group I component (g/L): EDTA 5, feSO 4 ·7H 2 O5, trace element II (g/L): EDTA 15, cuSO 4 ·5H 2 O 0.2、ZnSO 4 ·7H 2 O 0.43、CoCl 2 ·6H 2 O 0.24、MnCl 2 ·4H 2 O 0.99、Na 2 MoO 4 ·2H 2 O0.22、NiCl 2 ·6H 2 O 0.19、NaSeO 4 0.11、H 3 BO 4 0.014。
MBR adopts polyvinylidene fluoride (PVDF) hollow fiber membrane (average pore diameter 0.01um, specific surface area 0.06m 2 ) And is provided with a perforated aeration device. The filtration flow of MBR is constant to 15L/(m) 2 H). Extracting filtrate from the membrane module by a pump for a period of 10 minutes, namely 9 minutes for rest for 1 minute; the suspension solid concentration of the mixed solution is kept between 7000 and 8000mg/L. The sludge retention time was 30 days. The reactor temperature was maintained at about 24℃and the pH was maintained at about 7.3. The wavelength of the ultraviolet lamp is 320-380nm. A pressure sensor is installed to detect the transmembrane pressure TMP. When the TMP value increased to 40kpa, the membrane module was removed for cleaning (tap water rinse, 1% NaClO solution wash, 10% citric acid soak for 8 hours).
The invention has the action principle that: the attachment and growth of bacteria on the membrane surface results in the formation of biofilms, which are the primary cause of membrane contamination. Biofilm formation is generally due to a phenomenon known as Quorum Sensing (QS). During QS, bacteria perform various types of group behaviors, including biofilm formation, through secretion of signal molecules. Degradation of signal molecules, namely, population quenching (QQ), is an effective method for alleviating membrane fouling. Population quenching populations degrade signal molecules by secreting population quenching enzymes. Under the irradiation of ultraviolet light, the nitrate in the solution can generate hydroxyl radical degradation signal molecules. Therefore, the invention reduces the QS effect of microorganisms in MBR by adding the group quenching bacteria capable of degrading AHLs and ultraviolet irradiation, slows down the formation of biological films on the films, and further relieves the film pollution.
Compared with the prior art, the invention has the following advantages and effects:
(1) And simultaneously, degrading the signal molecules by utilizing the group quenching bacteria and ultraviolet light.
(2) The operation process is simple and easy to realize.
Drawings
FIG. 1 shows the filtration cycle of each reactor (1 square corresponds to one filtration cycle and then is cleaned, the longer the square corresponds to, the less contaminated), R1: controlling MBR; r2: adding MBR of the population quenching bacteria population; r3: an ultraviolet irradiated MBR; r4: MBR was added to the population-quenched population while simultaneously irradiating with ultraviolet light.
Fig. 2 is a corresponding experimental set-up.
1, a water inlet tank; 2 peristaltic pump; 3, a membrane component; 4, an aeration device; 5 a pressure sensor; and 6, an ultraviolet lamp.
Detailed Description
Following the above technical solutions, the following descriptions of the present invention will be further described in detail with reference to the accompanying drawings and examples, and it should be noted that the present invention is not limited to the following specific embodiments, and all equivalent changes made on the basis of the technical solutions of the present application fall within the protection scope of the present invention. The present invention will be described in further detail with reference to examples.
Example 1:
influence of the group quenching group on MBR Membrane pollution
Four MBR reactors are arranged, namely a control MBR (R1), an MBR (R2) added with a group quenching sterilization group, an MBR (R3) irradiated by ultraviolet light and an MBR (R4) simultaneously added with a group quenching sterilization group and irradiated by ultraviolet light.
Four MBRs run in parallel, and the effective volume of each MBR is 6L. Synthetic wastewater is pumped into the MBR by peristaltic pumps. Theoretical amount of main nutrient substance of synthetic wastewater: COD is 320mg/L, ammonia nitrogen is 40mg/L, and the rest is added according to the requirement based on actual measurement. Microelements I and II are 1mL/L respectively. Microelement group I component (g/L): EDTA 5, feSO 4 ·7H 2 And O5. Trace element II component (g/L): EDTA 15, cuSO 4 ·5H 2 O 0.2、ZnSO 4 ·7H 2 O 0.43、CoCl 2 ·6H 2 O 0.24、MnCl 2 ·4H 2 O 0.99、Na 2 MoO 4 ·2H 2 O 0.22、NiCl 2 ·6H 2 O0.19、NaSeO 4 0.11、H 3 BO 4 0.014.MBR adopts polyvinylidene fluoride (PVDF) hollow fiber membrane (average pore diameter 0.01um, specific surface area 0.06m 2 ) And is provided with a perforated aeration device. The filtration flow of MBR is constant to 15L/(m) 2 H). From film mould by pumpThe filtrate was extracted from the block for 10 minutes (9 minutes of extraction, 1 minute of rest). The wavelength of the ultraviolet lamp is 320-380nm. The suspension solid concentration of the mixed solution is kept between 7000 and 8000mg/L. The sludge retention time was 30 days. The reactor temperature was maintained at about 24℃and the pH was maintained at about 7.3. A pressure sensor is installed to detect transmembrane pressure (TMP). When the TMP value increased to 40kpa, the membrane module was removed for cleaning (tap water rinse, 1% NaClO solution wash, 10% citric acid soak for 8 hours).
Results and analysis
Example 1
As shown in FIG. 1, the average filtration period for R1 was 3.50 days, the average filtration period for R2 was 5.49 days, the average filtration period for R3 was 5.51 days, and the average filtration period for R4 was 8.34 days. Therefore, the addition of the group quenching bacteria or the ultraviolet irradiation of the MBR can effectively prolong the filtration period of the MBR, and the effect is similar. Importantly, the average filtration period of R4 is much higher than R2 and R3, indicating that the simultaneous addition of the population quenching clusters and irradiation of the MBR with uv light is most significant in alleviating membrane fouling.
Claims (1)
1. A method for comprehensively reducing membrane pollution of a membrane bioreactor, which is characterized in that a group quenching bacteria group capable of degrading signal molecules is added into an MBR, and ultraviolet irradiation is carried out on the MBR, and the specific operation steps and process conditions are as follows:
(1) First enriching a population quenching population capable of degrading signal molecules; specifically, sludge in the MBR which is operated stably is taken as a sludge sample, and PBS is used for pretreatment of the sludge; quenching the population with a concentrated population of C8-HSL, adding the pretreated sludge to a conical flask containing 100mL minimal medium and 20 mM of C8-HSL, and culturing at 30℃and 150rpm for three days; after three days, 10% of the culture solution was taken and cultured in a new medium, and the procedure was repeated three times, with a new medium composition: 100mL minimal medium and 20 mM C8-HSL; the composition of the minimal medium was as follows: naCl 1g/L, KCl 0.5g/L and MgCl 2 0.4 g/L,CaCl 2 0.1 g/L,Na 2 SO 4 0.15 g/L,KH 2 PO 4 2 g/L,Na 2 HPO 4 2.25 g/L; finally, centrifuging the culture solution at 8000rpm for 5 min; the supernatant was discarded, the bottom bacterial pellet was added to a conical flask containing 100mL LB medium and incubated to OD 600 1.0 for subsequent experiments;
(2) The selected flora was then added daily to a stably running MBR with an effective volume of 6L and a daily added volume of 10mL; the water in the whole MBR system is always irradiated by an ultraviolet lamp, and the wavelength of the ultraviolet lamp is 320-380 nm;
step (2), pumping synthetic wastewater into MBR by a peristaltic pump, wherein the COD of the synthetic wastewater is 320mg/L, the ammonia nitrogen is 40mg/L, and the microelements I and II are 1mL/L respectively; microelement I group composition: EDTA 5g/L, feSO 4 ·7H 2 O5 g/L, trace element II composition: EDTA 15 g/L, cuSO 4 ·5H 2 O 0.2 g/L、ZnSO 4 ·7H 2 O 0.43 g/L、CoCl 2 ·6H 2 O 0.24 g/L、MnCl 2 ·4H 2 O 0.99 g/L、Na 2 MoO 4 ·2H 2 O 0.22 g/L、NiCl 2 ·6H 2 O 0.19 g/L、NaSeO 4 0.11 g/L、H 3 BO 4 0.014 g/L;
The MBR adopts a polyvinylidene fluoride hollow fiber membrane and is provided with a perforated aeration device, and the filtration flow of the MBR is constant to 15L/(m) 2 H), extracting filtrate from the membrane module by a pump, wherein the extraction period is 10 minutes, namely 9 minutes, and rest for 1 minute; the concentration of suspended solids in the mixed solution is kept at 7000-8000mg/L, the residence time of sludge is 30 days, the temperature of the reactor is maintained at 24 ℃, the pH is maintained at 7.3, a pressure sensor is arranged to detect the transmembrane pressure TMP, when the TMP value is increased to 40kpa, the membrane module is taken out for cleaning, the cleaning is flushed by tap water, the reactor is washed by 1% NaClO solution, and the reactor is soaked by 10% citric acid for 8 hours.
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105296678A (en) * | 2015-11-26 | 2016-02-03 | 江苏大学 | Biomembrane control method based on photocatalytic quenching quorum sensing signal |
CN107158957A (en) * | 2017-03-22 | 2017-09-15 | 北京大学深圳研究生院 | The method that immobilization bacterial strain controls fouling membrane is quenched in a kind of utilization quorum sensing |
KR20200112467A (en) * | 2019-03-22 | 2020-10-05 | 경북대학교 산학협력단 | Method for microbial quorum quenching by light irradiation and method for controlling biological pollution |
CN112939139A (en) * | 2021-03-04 | 2021-06-11 | 汕头大学 | Photocatalysis membrane reactor and sewage treatment system |
CN114455697A (en) * | 2022-03-16 | 2022-05-10 | 苏州盛虹环保科技有限公司 | MBR membrane pollution control method based on microbial population induction quenching |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105296678A (en) * | 2015-11-26 | 2016-02-03 | 江苏大学 | Biomembrane control method based on photocatalytic quenching quorum sensing signal |
CN107158957A (en) * | 2017-03-22 | 2017-09-15 | 北京大学深圳研究生院 | The method that immobilization bacterial strain controls fouling membrane is quenched in a kind of utilization quorum sensing |
KR20200112467A (en) * | 2019-03-22 | 2020-10-05 | 경북대학교 산학협력단 | Method for microbial quorum quenching by light irradiation and method for controlling biological pollution |
CN112939139A (en) * | 2021-03-04 | 2021-06-11 | 汕头大学 | Photocatalysis membrane reactor and sewage treatment system |
CN114455697A (en) * | 2022-03-16 | 2022-05-10 | 苏州盛虹环保科技有限公司 | MBR membrane pollution control method based on microbial population induction quenching |
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