CN115700090A - Edible fungus extract composition for improving sleep and application thereof - Google Patents
Edible fungus extract composition for improving sleep and application thereof Download PDFInfo
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Abstract
The invention discloses an edible fungus extract composition for improving sleep and application thereof, wherein the effective components comprise the following components in parts by weight: the preparation method of the composition comprises the steps of respectively crushing, water extracting, alcohol extracting, concentrating and drying to obtain the ganoderma lucidum composite extract and the hericium erinaceus composite extract. The edible fungus compound extract is refined by modern technology under the guidance of traditional medicine and modern traditional Chinese medicine health preserving theory, the compatibility is reasonable, the process is simple, the prepared edible fungus compound extract is rich in active ingredients such as polysaccharide, triterpene and the like, and can be prepared into various dosage forms such as granules, capsules, tablets, powder and the like by adding auxiliary materials, thereby having the effect of improving sleep.
Description
Technical Field
The invention belongs to the technical field of food and health-care food, and particularly relates to an edible fungus extract composition for improving sleep and application thereof.
Background
With the rapid development of economic society in China, various competitions are aggravated, the rhythm of life is accelerated continuously, the spirit of people is always in a nervous state, the sleep quality is seriously influenced, and good sleep becomes luxury in daily life. Although the sleep problem does not belong to critical diseases, the harm caused by the sleep problem is self-evident, and the diseases such as hypertension, myocardial infarction, hyperlipidemia, obesity, even canceration and the like can be induced, and anxiety, depression, even hallucinations and other mental symptoms can be caused. The intractable sleep disorder problem can bring long-term pain to people. The sedative-hypnotic drugs are generally used for prolonging sleep clinically, but the sedative-hypnotic drugs can be dependent on the drugs, have obvious side effects and have adverse reactions of dizziness, fatigue, drowsiness, inattention, even temporary amnesia and the like in different degrees, so that the functional health food with the sleep improving effect has great market potential.
The edible fungus is rich in nutritional ingredients such as protein and dietary fiber, and also contains bioactive ingredients such as polysaccharide, terpenes, alkaloids and sterol. Ganoderma lucidum and Hericium erinaceus are common edible and medicinal fungi. Ganoderma lucidum is regarded as a magical treasure for nourishing and strengthening body resistance by modern medicians and is used for treating unsteadiness of heart-mind, insomnia and palpitation, cough and asthma due to lung deficiency, consumptive disease and shortness of breath and poor appetite. Hericium erinaceus is a large fungus used as both medicine and food, and has effects of benefiting five internal organs, promoting digestion, invigorating spleen and qi, and nourishing body. The hericium erinaceus has obvious effects of nourishing the stomach, protecting the intestines and maintaining the health of a digestive system, has a protective effect on a nervous system, can promote the synthesis of nerve growth factors, and can improve symptoms such as cognitive disorder, depression, anxiety, insomnia and the like.
Disclosure of Invention
Based on the above disadvantages and shortcomings in the prior art, the present invention provides an edible fungus extract composition for improving sleep and its application.
In order to achieve the purpose, the invention adopts the following technical scheme:
the edible fungus extract composition for improving sleep comprises the following effective components in parts by weight: 5-20 parts of ganoderma lucidum composite extract and 20-60 parts of hericium erinaceus composite extract, preferably 8-15 parts of ganoderma lucidum composite extract and 30-45 parts of hericium erinaceus composite extract.
Preferably, the preparation method of the ganoderma lucidum compound extract comprises the following steps: taking ganoderma lucidum sporocarp raw materials, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding purified water with the volume 10-40 times that of the raw materials, heating, boiling, extracting for 2-3 hours, performing filter pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding edible alcohol with the mass fraction of 60-95% and the volume 5-20 times that of the raw materials, heating to 70-95 ℃, extracting for 2-3 hours, performing filter pressing to obtain alcohol extraction filtrate, performing reduced pressure concentration on the water extraction filtrate at 60-90 ℃, collecting water extraction concentrated solution, concentrating the alcohol extraction filtrate at 65-85 ℃, recovering alcohol and collecting alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating for 30 minutes at 100 ℃, performing spray drying at the air inlet temperature of 160-200 ℃ and the air outlet temperature of 70-100 ℃, and collecting the ganoderma lucidum composite extract.
Preferably, the preparation method of the hericium erinaceus compound extract comprises the following steps: taking hericium erinaceus sporophore raw materials, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting the coarse powder into an extraction tank, adding purified water with the volume 20-50 times that of the raw materials, heating, boiling, extracting for 2-3 hours, performing filter pressing to obtain water extraction filtrate and filter residues, putting the filter residues into the extraction tank again, adding edible alcohol with the mass fraction of 60-95% and the volume 10-30 times that of the raw materials, heating to 70-95 ℃, extracting for 2-3 hours, performing filter pressing to obtain alcohol extraction filtrate, performing reduced pressure concentration on the water extraction filtrate at 60-90 ℃, collecting the water extraction concentrated solution, concentrating the alcohol extraction filtrate at 65-85 ℃, recovering the alcohol and collecting the alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating for 30 minutes at 100 ℃, performing spray drying at the air inlet temperature of 160-200 ℃ and the air outlet temperature of 70-100 ℃, and collecting the hericium erinaceus composite extract.
Preferably, before the alcohol extraction of the filter residue, the method further comprises the following steps: adding purified water into the filter residue of the lucid ganoderma or the filter residue of the hericium erinaceus obtained by filter pressing, extracting for 2-3 hours, then carrying out filter pressing again, and combining the filtrate obtained by the two water extractions. Preferably, before alcohol extraction of the filter residue, adding purified water with the volume of 20-50 times of the material amount into the filter residue of the ganoderma lucidum or the filter residue of the hericium erinaceus obtained by pressure filtration, extracting for 2-3 hours, then carrying out pressure filtration again, and combining the two water extraction filtrates.
Preferably, the ganoderma lucidum filter residue or the hericium erinaceus filter residue obtained by pressure filtration after alcohol extraction is added with edible alcohol for extraction for 2-3 h, then pressure filtration is carried out again, and the two alcohol extraction filtrates are combined. Preferably, the ganoderma lucidum filter residue or the hericium erinaceus filter residue obtained by pressure filtration after alcohol extraction is added with edible alcohol with the mass fraction of 60-95% of 10-30 times of the volume of the feed amount, extracted for 2-3 hours, then pressure filtration is carried out again, and the two alcohol extraction filtrates are combined.
Preferably, the content of the polysaccharide in the ganoderma lucidum compound extract is 15-30%, and the content of the total triterpene is 2.5-5.5%.
Preferably, the content of polysaccharide in the hericium erinaceus compound extract is 15-40%, and the content of total triterpene is 1.5-4.0%.
The invention also provides application of the edible fungus extract composition in preparation of edible fungus extract preparations, wherein the preparations comprise powder, granules, capsules or tablets.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention provides an edible fungus extract composition taking ganoderma lucidum and hericium erinaceus as raw materials, which is extracted by different extraction solvents to extract active ingredients of the edible fungus extract composition to play a role in improving sleep synergistically.
2. In the preparation process of the ganoderma lucidum composite extract and the hericium erinaceus composite extract, water is adopted to extract active ingredients such as water-soluble active polysaccharide of the ganoderma lucidum composite extract and the hericium erinaceus composite extract, ethanol is adopted to extract fat-soluble active ingredients such as total triterpenes, and water extraction and alcohol extraction are adopted to extract the active ingredients of the ganoderma lucidum and the hericium erinaceus in a composite mode.
3. The extraction process of the lucid ganoderma compound extract and the hericium erinaceus compound extract in the edible fungus extract composition is simple and feasible, other components are not added, side effects caused by the introduction of other components are avoided, and the edible fungus extract composition is safe and effective.
4. The composition provided by the invention can be added with auxiliary materials to prepare various solid preparation formulations such as powder, granules, capsules and tablets, and has the effect of improving sleep.
Detailed Description
The technical solution of the present invention is further explained by the following specific examples.
Example 1:
the edible fungus extract composition for improving sleep comprises the following effective components in parts by weight: 15 parts of ganoderma lucidum composite extract and 40 parts of hericium erinaceus composite extract;
the preparation of the ganoderma lucidum composite extract comprises the following steps: taking 100 kg of ganoderma lucidum fruit body raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding 2000L of purified water, heating, boiling and extracting for 3 hours, carrying out filter pressing to obtain water-extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 800L of edible alcohol with the mass fraction of 90%, heating to 90 ℃, extracting for 3 hours, carrying out filter pressing to obtain alcohol-extraction filtrate, carrying out reduced pressure concentration on the water-extraction filtrate at 80 ℃, collecting water-extraction concentrated solution, concentrating and recovering alcohol from the alcohol-extraction filtrate at 85 ℃, collecting the alcohol-extraction concentrated solution, combining the water-extraction concentrated solution and the alcohol-extraction concentrated solution, uniformly mixing, preheating for 30 min at 100 ℃, carrying out spray drying at the air inlet temperature of 180 ℃ and the air outlet temperature of 85 ℃, and collecting to obtain 7.8 kg of ganoderma lucidum composite extract, wherein the polysaccharide content, the total triterpene content and the total flavone content are detected to be 24.3% and 1.35%.
The preparation method of the hericium erinaceus composite extract comprises the following steps: taking 100 kg of hericium erinaceus sporophore raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding 4000L of purified water, heating, boiling, extracting for 2 hours, performing filter pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 2000L of edible alcohol with the mass fraction of 90%, heating to 90 ℃, extracting for 3 hours, performing filter pressing to obtain alcohol extraction filtrate, performing reduced pressure concentration on the water extraction filtrate at 80 ℃, collecting water extraction concentrate, concentrating the alcohol extraction filtrate at 85 ℃, recovering the alcohol, collecting the alcohol extraction concentrate, combining the water extraction concentrate and the alcohol extraction concentrate, uniformly mixing, preheating for 30 min at 100 ℃, performing spray drying at the conditions of air inlet temperature of 195 ℃ and air outlet temperature of 90 ℃, and collecting to obtain 19.6 kg of hericium erinaceus composite extract, wherein the polysaccharide content is 25.6%, the total triterpene content is 3.5%, the total flavone is 0.23%, and the ergothioneine is 0.14% by detection.
Example 2 an edible fungus extract composition for improving sleep comprises the following effective ingredients in parts by weight: 10 parts of lucid ganoderma compound extract and 45 parts of hericium erinaceus compound extract;
the preparation of the ganoderma lucidum composite extract comprises the following steps: taking 200 kg of ganoderma lucidum fruiting body raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding 3000L of purified water, heating, boiling and extracting for 3 h, filter-pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 3000L of purified water, heating, boiling and extracting for 3 h, filter-pressing again to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 1500L of edible alcohol with the mass fraction of 95%, heating to 90 ℃ for extracting for 3 h, filter-pressing to obtain alcohol extraction filtrate, combining the two times of water extraction filtrate, concentrating under 80 ℃ under reduced pressure, collecting the water extraction concentrated solution, concentrating the alcohol extraction filtrate at 85 ℃ to recover alcohol, collecting the alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating for 30 min at 100 ℃, spray-drying under the conditions of air inlet temperature of 185 ℃ and air outlet temperature of 90 ℃, collecting 18.5 kg of ganoderma lucidum composite extract, detecting the polysaccharide content of 26.2%, the total triterpene content of 4.5% and 1.42% of total flavone.
The preparation method of the hericium erinaceus compound extract comprises the following steps: taking 100 kg of hericium erinaceus sporophore raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting the coarse powder into an extraction tank, adding 3000L of purified water, heating, boiling and extracting for 2 hours, filter-pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 3000L of purified water, heating, boiling and extracting for 2 hours, filter-pressing again to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 1500L of edible alcohol with the mass fraction of 90%, heating to 90 ℃ for extracting for 3 hours, filter-pressing to obtain alcohol extraction filtrate, combining the two times of water extraction filtrate, concentrating at 80 ℃, collecting the water extraction concentrated solution, concentrating the alcohol extraction filtrate at 85 ℃, recovering alcohol, collecting alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating at 100 ℃ for 30 minutes, then spray-drying at the air inlet temperature of 190 ℃ and the air outlet temperature of 85 ℃, collecting 22.5 kg of hericium erinaceus composite extract, detecting that the polysaccharide content is 24.7%, the total triterpene content is 3.7%, the total flavone is 0.7%, the total triterpene content is 0.24%, and 0.16% of ergothionein the ergothioneine.
Example 3: the edible fungus extract composition for improving sleep comprises the following effective components in parts by weight: 15 parts of ganoderma lucidum composite extract and 35 parts of hericium erinaceus composite extract;
the preparation of the ganoderma lucidum composite extract comprises the following steps: taking 200 kg of ganoderma lucidum fruiting body raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding 3000L of purified water, heating, boiling and extracting for 3 h, filter-pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 3000L of purified water, heating, boiling and extracting for 2h, filter-pressing again to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 1500L of edible alcohol with the mass fraction of 95%, heating to 90 ℃ for extracting for 3 h, filter-pressing again to obtain alcohol extraction filtrate, combining the two water extraction filtrates, concentrating at 80 ℃ under reduced pressure, collecting water extraction concentrated solution, combining the two alcohol extraction filtrates, concentrating at 85 ℃, recovering alcohol and collecting alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating at 100 ℃ for 30 min, then performing spray drying at the air inlet temperature of 190 ℃ and the air outlet temperature of 90 ℃, collecting 21.6 kg of ganoderma lucidum composite extract, detecting that the polysaccharide content is 27.3%, and the total triterpene content is 4.6%. 1.55 percent of total flavonoids.
The preparation method of the hericium erinaceus composite extract comprises the following steps: taking 100 kg of hericium erinaceus sporophore raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting the coarse powder into an extraction tank, adding 3500L of purified water, heating, boiling, extracting for 2 hours, performing filter pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 1500L of edible alcohol with the mass fraction of 95%, heating to 85 ℃, extracting for 3 hours, performing filter pressing to obtain alcohol extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 1500L of edible alcohol with the mass fraction of 90%, heating to 85 ℃, extracting for 2 hours, performing filter pressing again to obtain alcohol extraction filtrate, performing reduced pressure concentration on the water extraction filtrate at 85 ℃, collecting water extraction concentrated solution, combining the two alcohol extraction filtrates, concentrating at 80 ℃, recovering alcohol, collecting alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating for 30 minutes at 100 ℃, performing spray drying at the air inlet temperature of 190 ℃ and the air outlet temperature of 90 ℃, collecting 20.9 kg of hericium erinaceus composite extract, detecting that the polysaccharide content is 24.1%, the total triterpene content is 4.1%, the total flavone is 0.25%, and the ergothioneine is 0.17%.
Comparative example 1:
the edible fungus extract composition for improving sleep comprises the following effective components in parts by weight: 15 parts of ganoderma lucidum water extract and 40 parts of hericium erinaceus water extract, wherein the preparation method of the ganoderma lucidum composite extract comprises the following steps: selecting 100 kg of ganoderma lucidum fruiting body raw material, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding 2000L of purified water, heating, boiling, extracting for 3 h, press-filtering to obtain water extract filtrate, carrying out reduced pressure concentration on the water extract filtrate at 80 ℃, collecting water extract concentrated solution, and carrying out spray drying on the concentrated solution to obtain the ganoderma lucidum water extract, wherein the polysaccharide content is 23.9%, the total triterpene content is 1.1%, and the total flavone content is 0.37% by detection.
Preparing a hericium erinaceus composite extract: taking 100 kg of hericium erinaceus sporophore raw material, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding 4000L of purified water, heating, boiling and extracting for 2 hours, filter-pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding 4000L of purified water, heating, boiling and extracting for 2 hours, filter-pressing again to obtain water extraction filtrate, carrying out reduced pressure concentration on the water extraction filtrate at 80 ℃, collecting water extraction concentrated solution, and carrying out spray drying on the concentrated solution to obtain the hericium erinaceus water extract, wherein the polysaccharide content is 21.7%, the total triterpene content is 0.9%, the total flavone content is 0.12%, and the ergothioneine content is 0.07%.
Example 4:
adding 40 parts of soluble starch into the edible fungus extract composition prepared in the examples 1-3 and the comparative example 1, putting the mixture into a high-efficiency wet granulator, adding 95% ethanol to prepare a soft material, granulating the prepared soft material by using a granulator, and drying to prepare the edible fungus extract granules 1, the edible fungus extract granules 2, the edible fungus extract granules 3 and the comparative example edible fungus extract granules.
Example 5:
the granules prepared in example 4 were filled into capsules by a full-automatic capsule filling machine, and capsules were prepared.
Example 6:
taking 15 parts of the ganoderma lucidum composite extract prepared in the example 2 and 40 parts of the hericium erinaceus composite extract prepared in the example 4, adding 30 parts of maltodextrin, 15 parts of microcrystalline cellulose, 7 parts of magnesium stearate and 3 parts of silicon dioxide, putting into a mixer, fully mixing, and filling capsules by using a full-automatic capsule filling machine to prepare capsules.
Example 7:
taking the edible fungus extract composition prepared in example 2, adding 30 parts of maltodextrin, putting into a high-efficiency wet granulator, adding 95% ethanol to prepare soft materials, granulating the prepared soft materials by using a granulator, drying to prepare granules, mixing with 5 parts of magnesium stearate, and tabletting by using a tabletting machine to prepare tablets.
Example 8
1. Influence of granular edible fungus extract on direct sleep of mice
75 mice with the weight of 16.0-18.0 g are randomly divided into a control group, an edible fungus extract granule 1 group, an edible fungus extract granule 2 group, an edible fungus extract granule 3 group and a comparative edible fungus granule group, wherein each group comprises 15 mice. A control group is intragastrically administered with 20 mL/kg of 0.5% sodium carboxymethylcellulose every day, and a comparative example edible fungus granule group, an edible fungus extract granule group 1, an edible fungus extract granule group 2 and an edible fungus extract granule group 3 are intragastrically administered with 0.5 mg/kg of edible fungus granules, edible fungus extract granules 1, edible fungus extract granules 2 and edible fungus extract granules 3 respectively, and the administration time is 1 time per day, and the intragastrically is continuously administered for 30 days; and (5) observing whether each group of mice has sleep phenomena 15 min after the last drug, and recording the number of animals falling asleep and the sleep time.
And (4) conclusion: compared with a control group, the group of the edible fungus granules of the comparative example, the group of the edible fungus extract granules 1, the group of the edible fungus extract granules 2 and the group of the edible fungus extract granules 3 have no direct sleep effect after being continuously gavaged for 30 days.
2. Effect of granular edible fungus extract on sleep time of sodium pentobarbital in threshold dose
75 mice with the weight of 16.0-18.0 g are randomly divided into a control group, an edible fungus extract granule group 1, an edible fungus extract granule group 2, an edible fungus extract granule group 3 and a comparative edible fungus granule group, and each group comprises 15 mice. The control group is administrated with 20 mL/kg of 0.5% sodium carboxymethylcellulose by intragastric administration every day, the comparative example edible fungus granule group, the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 are administrated with the comparative example edible fungus granule, the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 by intragastric administration respectively, the dosage is 0.5 mg/kg, 1 time every day, and the intragastric administration is continuously carried out for 30 days. 15 min after the last dose of the medicine, 43.5 mg/kg of sodium pentobarbital is injected into the abdominal cavity of each group of animals (the dose of sodium pentobarbital which enables the animals to fall asleep 100% and does not lead to overlong sleep time is determined by a pre-test), the injection amount is 10 mL/kg, and whether the sleep time of the sodium pentobarbital with the threshold dose can be prolonged by using the disappearance of the positive turning reflex as an index of the edible fungus extract granules is observed.
And (4) conclusion: compared with the control group, the sleep time of the mice of the comparative example edible fungus granule group has no significant difference (P is more than 0.05), the sleep time of the mice of the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 is obviously prolonged (P is less than 0.05), and the results are shown in the table 1.
TABLE 1
| Sleep time (min) | |
| Control group | 28.5 |
| Comparative example granular formulation of edible fungus | 32.9 |
| Edible mushroom extract granule 1 group | 36.9* |
| Edible mushroom extract granule 2 groups | 37.5* |
| Edible mushroom extract granules 3 groups | 38.7* |
P <0.05 compared to control group.
3. Effect of edible fungus extract granules on sleep incidence of sodium pentobarbital in subthreshold dose
75 mice with the weight of 16.0-18.0 g are randomly divided into a control group, an edible fungus extract granule group 1, an edible fungus extract granule group 2, an edible fungus extract granule group 3 and a comparative edible fungus granule group, and each group comprises 15 mice. The control group is administrated with 20 mL/kg of 0.5% sodium carboxymethylcellulose by intragastric administration every day, the comparative example edible fungus granule group, the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 are administrated with the comparative example edible fungus granule, the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 by intragastric administration respectively, the dosage is 0.5 mg/kg, 1 time every day, and the intragastric administration is continuously carried out for 30 days. And (3) injecting 24 mg/kg of pentobarbital sodium into the abdominal cavity of each group of animals 15 min after the last dose of the drug (the sub-threshold dose of the pentobarbital sodium which ensures that 90 percent of mice do not disappear after turning normal reflex is determined by a pre-test), wherein the injection amount is 10 mL/kg, the number of the animals falling asleep within 30 min (the animals with turning normal reflex reaching more than 1 min) is recorded, and the incidence rate of the animals falling asleep is calculated.
And (4) conclusion: compared with a control group, the sleep incidence rates of the edible fungus granule group, the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 are obviously improved (P is less than 0.05); the sleep incidence of the edible fungus extract granule group 1, the edible fungus extract granule group 2 and the edible fungus extract granule group 3 is higher than that of the comparative edible fungus granule group, and the results are shown in table 2.
TABLE 2
| Number of mice sleeping (only) | Incidence of sleep (%) | |
| Control group | 1 | 6.7 |
| Comparative example granular formulation of edible fungus | 4 | 26.7* |
| Edible mushroom extract granule 1 group | 7 | 46.7* |
| Edible mushroom extract granules 2 groups | 8 | 53.3* |
| Edible mushroom extract granules 3 groups | 9 | 60.0* |
P <0.05 compared to control group.
The foregoing has outlined rather broadly the preferred embodiments and principles of the present invention and it will be appreciated that those skilled in the art may devise variations of the present invention that are within the spirit and scope of the appended claims.
Claims (9)
1. The edible fungus extract composition for improving sleep is characterized by comprising the following effective components in parts by weight: 5 to 20 parts of lucid ganoderma compound extract and 20 to 60 parts of hericium erinaceus compound extract.
2. The edible fungus extract composition for improving sleep as claimed in claim 1, which comprises the following effective components in parts by weight: 8-15 parts of lucid ganoderma compound extract and 30-45 parts of hericium erinaceus compound extract.
3. The edible fungus extract composition for improving sleep as claimed in claim 1 or 2, wherein the preparation of the ganoderma lucidum composite extract comprises the steps of: taking ganoderma lucidum sporocarp raw materials, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting into an extraction tank, adding purified water with the volume 10-40 times that of the raw materials, heating, boiling, extracting for 2-3 hours, performing filter pressing to obtain water extraction filtrate and filter residue, putting the filter residue into the extraction tank again, adding edible alcohol with the mass fraction of 60-95% and the volume 5-20 times that of the raw materials, heating to 70-95 ℃, extracting for 2-3 hours, performing filter pressing to obtain alcohol extraction filtrate, performing reduced pressure concentration on the water extraction filtrate at 60-90 ℃, collecting water extraction concentrated solution, concentrating the alcohol extraction filtrate at 65-85 ℃, recovering alcohol and collecting alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating for 30 minutes at 100 ℃, performing spray drying at the air inlet temperature of 160-200 ℃ and the air outlet temperature of 70-100 ℃, and collecting the ganoderma lucidum composite extract.
4. The sleep-improving edible fungus extract composition according to claim 1 or 2, wherein the hericium erinaceus compound extract is prepared by the steps of: taking hericium erinaceus sporophore raw materials, selecting, removing impurities, crushing into 1-5 mm coarse powder, putting the coarse powder into an extraction tank, adding purified water with the volume 20-50 times that of the raw materials, heating, boiling, extracting for 2-3 hours, performing filter pressing to obtain water extraction filtrate and filter residues, putting the filter residues into the extraction tank again, adding edible alcohol with the mass fraction of 60-95% and the volume 10-30 times that of the raw materials, heating to 70-95 ℃, extracting for 2-3 hours, performing filter pressing to obtain alcohol extraction filtrate, performing reduced pressure concentration on the water extraction filtrate at 60-90 ℃, collecting the water extraction concentrated solution, concentrating the alcohol extraction filtrate at 65-85 ℃, recovering the alcohol and collecting the alcohol extraction concentrated solution, combining the water extraction concentrated solution and the alcohol extraction concentrated solution, uniformly mixing, preheating for 30 minutes at 100 ℃, performing spray drying at the air inlet temperature of 160-200 ℃ and the air outlet temperature of 70-100 ℃, and collecting the hericium erinaceus composite extract.
5. The edible fungus extract composition according to claim 3 or 4, further comprising, before the alcohol extraction of the residue: adding purified water into the filter residue of the lucid ganoderma or the filter residue of the hericium erinaceus obtained by filter pressing, extracting for 2-3 hours, then carrying out filter pressing again, and combining the filtrate obtained by the two water extractions.
6. The edible fungus extract composition according to claim 3 or 4, further comprising: and adding edible alcohol into the ganoderma lucidum filter residue or the hericium erinaceus filter residue obtained by filter pressing after alcohol extraction, extracting for 2-3 h, then carrying out filter pressing again, and combining the two alcohol extraction filtrates.
7. The edible fungus extract composition according to claim 1 or 2, wherein the ganoderma lucidum composite extract polysaccharide content is 15-30%, and the total triterpene content is 2.5-5.5%.
8. The edible mushroom extract composition as claimed in claim 1 or 2, wherein the hericium erinaceus compound extract contains 15-40% of polysaccharide and 1.5-4.0% of total triterpenoids.
9. Use of an edible fungus extract composition according to claim 1 for the preparation of an edible fungus extract preparation, wherein the preparation comprises a powder, a granule, a capsule or a tablet.
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| JP2006036695A (en) * | 2004-07-27 | 2006-02-09 | Hirose Yukihiro | Composition exhibiting action of promoting good sleep and drink containing the same |
| CN104397535A (en) * | 2014-12-05 | 2015-03-11 | 黑龙江众生生物工程有限公司 | Composite edible-medicinal fungal oral liquid for enhancing immunity and production method for composite edible-medicinal fungal oral liquid |
| CN105053782A (en) * | 2015-07-14 | 2015-11-18 | 福建拓天生物科技有限公司 | Red rice instant powder with health-care functions and preparation method thereof |
| CN108853366A (en) * | 2018-09-28 | 2018-11-23 | 南宁常春藤肿瘤研究院 | A kind of drug that can promote and improve sleep quality and its production method |
| US20200023024A1 (en) * | 2018-07-20 | 2020-01-23 | Geneferm Biotechnology Co., Ltd. | Ganoderma lucidum polysaccharides composite composition |
| CN114868925A (en) * | 2022-05-19 | 2022-08-09 | 浙江百山祖生物科技有限公司 | Edible fungus polysaccharide extract composition and preparation method thereof |
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2022
- 2022-11-23 CN CN202211471591.1A patent/CN115700090A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1091257A (en) * | 1993-12-27 | 1994-08-31 | 叶世平 | " Houzhiling " nutrition oral liquid and manufacture method thereof |
| JP2006036695A (en) * | 2004-07-27 | 2006-02-09 | Hirose Yukihiro | Composition exhibiting action of promoting good sleep and drink containing the same |
| CN104397535A (en) * | 2014-12-05 | 2015-03-11 | 黑龙江众生生物工程有限公司 | Composite edible-medicinal fungal oral liquid for enhancing immunity and production method for composite edible-medicinal fungal oral liquid |
| CN105053782A (en) * | 2015-07-14 | 2015-11-18 | 福建拓天生物科技有限公司 | Red rice instant powder with health-care functions and preparation method thereof |
| US20200023024A1 (en) * | 2018-07-20 | 2020-01-23 | Geneferm Biotechnology Co., Ltd. | Ganoderma lucidum polysaccharides composite composition |
| CN108853366A (en) * | 2018-09-28 | 2018-11-23 | 南宁常春藤肿瘤研究院 | A kind of drug that can promote and improve sleep quality and its production method |
| CN114868925A (en) * | 2022-05-19 | 2022-08-09 | 浙江百山祖生物科技有限公司 | Edible fungus polysaccharide extract composition and preparation method thereof |
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