CN115698339A - 不固定条形码 - Google Patents

不固定条形码 Download PDF

Info

Publication number
CN115698339A
CN115698339A CN202180038991.0A CN202180038991A CN115698339A CN 115698339 A CN115698339 A CN 115698339A CN 202180038991 A CN202180038991 A CN 202180038991A CN 115698339 A CN115698339 A CN 115698339A
Authority
CN
China
Prior art keywords
sample
nucleotide
index position
barcode
molecular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180038991.0A
Other languages
English (en)
Chinese (zh)
Inventor
J·F·汤普森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Personal Genome Diagnostics Inc
Original Assignee
Personal Genome Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Personal Genome Diagnostics Inc filed Critical Personal Genome Diagnostics Inc
Publication of CN115698339A publication Critical patent/CN115698339A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00—Methods specially adapted for identifying library members
    • C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/185—Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119—Double strand sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
  • Luminescent Compositions (AREA)
  • Bidet-Like Cleaning Device And Other Flush Toilet Accessories (AREA)
CN202180038991.0A 2020-04-07 2021-04-06 不固定条形码 Pending CN115698339A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063006556P 2020-04-07 2020-04-07
US63/006,556 2020-04-07
PCT/US2021/026043 WO2021207267A1 (en) 2020-04-07 2021-04-06 Floating barcodes

Publications (1)

Publication Number Publication Date
CN115698339A true CN115698339A (zh) 2023-02-03

Family

ID=78023484

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180038991.0A Pending CN115698339A (zh) 2020-04-07 2021-04-06 不固定条形码

Country Status (11)

Country Link
US (1) US20230151356A1 (https=)
EP (1) EP4133110A4 (https=)
JP (1) JP2023521687A (https=)
KR (1) KR20220164753A (https=)
CN (1) CN115698339A (https=)
AU (1) AU2021251780A1 (https=)
BR (1) BR112022020164A2 (https=)
CA (1) CA3176915A1 (https=)
GB (1) GB2609801A (https=)
MX (1) MX2022012594A (https=)
WO (1) WO2021207267A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4427346A4 (en) * 2021-11-05 2025-07-09 Lenovo Beijing Ltd P-MPR REPORT MULTIPLE OF MAXIMUM AUTHORIZED EXPOSURE
CN113999893B (zh) * 2021-11-09 2022-11-01 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150072344A1 (en) * 2013-09-10 2015-03-12 Imdaptive Incorporated Barcoded Universal Marker Indicator (BUMI) Tags
US20180148715A1 (en) * 2010-10-08 2018-05-31 President And Fellows Of Harvard College High-Throughput Single Cell Barcoding
CN110023509A (zh) * 2016-11-15 2019-07-16 私人基因诊断公司 基因型分型测定中的非独特条形码

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3032613A1 (en) * 2016-11-10 2018-05-17 Takara Bio Usa, Inc. Methods of producing amplified double stranded deoxyribonucleic acids and compositions and kits for use therein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20180148715A1 (en) * 2010-10-08 2018-05-31 President And Fellows Of Harvard College High-Throughput Single Cell Barcoding
US20150072344A1 (en) * 2013-09-10 2015-03-12 Imdaptive Incorporated Barcoded Universal Marker Indicator (BUMI) Tags
CN110023509A (zh) * 2016-11-15 2019-07-16 私人基因诊断公司 基因型分型测定中的非独特条形码

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AARON POMERANTZ 等: "Real-time DNA barcoding in a rainforest using nanopore sequencing: opportunities for rapid biodiversity assessments and local capacity building", 《GIGASCIENCE》, vol. 7, no. 4, 2 April 2018 (2018-04-02) *

Also Published As

Publication number Publication date
WO2021207267A1 (en) 2021-10-14
AU2021251780A1 (en) 2022-10-20
CA3176915A1 (en) 2021-10-14
MX2022012594A (es) 2023-02-16
GB2609801A (en) 2023-02-15
GB202215530D0 (en) 2022-12-07
BR112022020164A2 (pt) 2022-11-22
EP4133110A4 (en) 2025-04-30
US20230151356A1 (en) 2023-05-18
JP2023521687A (ja) 2023-05-25
KR20220164753A (ko) 2022-12-13
EP4133110A1 (en) 2023-02-15

Similar Documents

Publication Publication Date Title
US20240068020A1 (en) Methods for next generation genome walking and related compositions and kits
CN110546272B (zh) 将衔接子附接至样品核酸的方法
CN107075581B (zh) 由靶向测序进行数字测量
US20230081899A1 (en) Modular nucleic acid adapters
CN110799653A (zh) 用于多重大规模平行测序的最佳索引序列
CN110832087A (zh) 用于多核苷酸样品的索引的通用短衔接子
CN113811618A (zh) 基于甲基化dna目标区域构建测序文库及系统和应用
KR20190116989A (ko) Hla 유전자를 위한 pcr 프라이머 세트, 및 이를 이용한 서열 분석 방법
CN109913458B (zh) circRNA及其在检测缺氧缺血性脑损伤中的应用
CN115698339A (zh) 不固定条形码
CN108949911B (zh) 鉴定和定量低频体细胞突变的方法
CN110872609B (zh) 对小rna分子精准建库和测序的方法以及应用
CN116144736A (zh) 甲状腺癌相关基因高通量扩增子文库的制备方法、多重pcr引物对及应用
CN108699591A (zh) 用于检测病毒性出血性败血病病毒的遗传突变的方法
CN111424096B (zh) 一种与胃腺癌发生发展相关的生物标志物
WO2022096768A1 (es) Método de fabricación de librerías de mirnas para secuenciación paralela masiva
CN107267600A (zh) 一种富集brca1和brca2基因目标区域的引物、方法、试剂盒及其应用
EP3455376B1 (en) Method for producing a plurality of dna probes and method for analyzing genomic dna using the dna probes
CN114144188B (zh) 放大及检测核糖核酸(rna)片段的方法
CN108251531B (zh) Ensg00000267549在判断骨肉瘤转移中的应用
CN118879867A (zh) 标志物检测试剂制备大熊猫睾丸肿瘤诊断试剂盒的应用
CN120442799A (zh) 一种检测pole基因多热点突变的引物组合、试剂盒及文库构建方法
CN110241213A (zh) 分子标志物及其在癌症中的应用
Barry Overcoming the challenges of applying target enrichment for translational research

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WD01 Invention patent application deemed withdrawn after publication
WD01 Invention patent application deemed withdrawn after publication

Application publication date: 20230203