MX2022012594A - Codigos de barras flotantes. - Google Patents

Codigos de barras flotantes.

Info

Publication number
MX2022012594A
MX2022012594A MX2022012594A MX2022012594A MX2022012594A MX 2022012594 A MX2022012594 A MX 2022012594A MX 2022012594 A MX2022012594 A MX 2022012594A MX 2022012594 A MX2022012594 A MX 2022012594A MX 2022012594 A MX2022012594 A MX 2022012594A
Authority
MX
Mexico
Prior art keywords
barcodes
floating
labeling
nucleic acid
methods
Prior art date
Application number
MX2022012594A
Other languages
English (en)
Inventor
John F Thompson
Original Assignee
Personal Genome Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Personal Genome Diagnostics Inc filed Critical Personal Genome Diagnostics Inc
Publication of MX2022012594A publication Critical patent/MX2022012594A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B20/00Methods specially adapted for identifying library members
    • C40B20/04Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/161Modifications characterised by incorporating target specific and non-target specific sites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/185Modifications characterised by incorporating bases where the precise position of the bases in the nucleic acid string is important
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/119Double strand sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/514Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electrochromic Elements, Electrophoresis, Or Variable Reflection Or Absorption Elements (AREA)
  • Luminescent Compositions (AREA)
  • Bidet-Like Cleaning Device And Other Flush Toilet Accessories (AREA)

Abstract

Se proporcionan en la presente sistemas y conjuntos de oligonucleótidos para etiquetar y analizar moléculas de ácido nucleico que incluyen códigos de barras de índice con números predeterminados de posiciones de índice. También se proporcionan en la presente métodos para etiquetar y analizar moléculas de ácido nucleico, así como métodos para identificar lecturas de secuencias erróneas usando los códigos de barras de muestra y moleculares descritos en la presente.
MX2022012594A 2020-04-07 2021-04-06 Codigos de barras flotantes. MX2022012594A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202063006556P 2020-04-07 2020-04-07
PCT/US2021/026043 WO2021207267A1 (en) 2020-04-07 2021-04-06 Floating barcodes

Publications (1)

Publication Number Publication Date
MX2022012594A true MX2022012594A (es) 2023-02-16

Family

ID=78023484

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022012594A MX2022012594A (es) 2020-04-07 2021-04-06 Codigos de barras flotantes.

Country Status (11)

Country Link
US (1) US20230151356A1 (es)
EP (1) EP4133110A1 (es)
JP (1) JP2023521687A (es)
KR (1) KR20220164753A (es)
CN (1) CN115698339A (es)
AU (1) AU2021251780A1 (es)
BR (1) BR112022020164A2 (es)
CA (1) CA3176915A1 (es)
GB (1) GB2609801A (es)
MX (1) MX2022012594A (es)
WO (1) WO2021207267A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113999893B (zh) * 2021-11-09 2022-11-01 纳昂达(南京)生物科技有限公司 兼容双测序平台的建库元件、试剂盒及建库方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2731460T3 (es) * 2010-10-08 2019-11-15 Harvard College Alto rendimiento de células individuales con código de barra
US20200248244A1 (en) * 2016-11-15 2020-08-06 Personal Genome Diagnostics Inc. Non-unique barcodes in a genotyping assay

Also Published As

Publication number Publication date
CA3176915A1 (en) 2021-10-14
WO2021207267A1 (en) 2021-10-14
CN115698339A (zh) 2023-02-03
AU2021251780A1 (en) 2022-10-20
JP2023521687A (ja) 2023-05-25
BR112022020164A2 (pt) 2022-11-22
EP4133110A1 (en) 2023-02-15
US20230151356A1 (en) 2023-05-18
GB2609801A (en) 2023-02-15
KR20220164753A (ko) 2022-12-13
GB202215530D0 (en) 2022-12-07

Similar Documents

Publication Publication Date Title
Gołębiewski et al. Generating amplicon reads for microbial community assessment with next‐generation sequencing
WO2018119452A3 (en) Methods and systems for analyzing nucleic acid molecules
ES2929281T3 (es) Métodos para el enriquecimiento de secuencias de ácidos nucleicos dirigidos con aplicaciones para la secuenciación de ácidos nucleicos con corrección de errores
Garrett-Bakelman et al. Enhanced reduced representation bisulfite sequencing for assessment of DNA methylation at base pair resolution
Lanzén et al. Exploring the composition and diversity of microbial communities at the Jan Mayen hydrothermal vent field using RNA and DNA
Duhaime et al. Towards quantitative metagenomics of wild viruses and other ultra‐low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method
GB2568608A (en) Personalized genetic testing
Francis et al. Changing expression patterns of TonB-dependent transporters suggest shifts in polysaccharide consumption over the course of a spring phytoplankton bloom
EP4265723A3 (en) Reagents and methods for molecular barcoding of nucleic acids of single cells
CN104293783A (zh) 适用于扩增子测序文库构建的引物、构建方法、扩增子文库及包含其的试剂盒
ATE403755T1 (de) Identifizierung einer erbb2- genexpressionssignatur bei brustkrebs
WO2011100617A3 (en) Nucleic acid, biomolecule and polymer identifier codes
ATE534748T1 (de) Direkter snp-nachweis mit nichtamplifizierter dna
EP2145180A4 (en) METHODS AND SYSTEMS FOR COMPARATIVE SEQUENCE ANALYSIS
CN104263726A (zh) 适用于扩增子测序文库构建的引物及扩增子测序文库的构建方法
WO2017201102A8 (en) Method of improved sequencing by strand identification
Bryson et al. Proteomic stable isotope probing reveals taxonomically distinct patterns in amino acid assimilation by coastal marine bacterioplankton
MX2022002458A (es) Metodo de aislamiento de nucleosomas circulantes.
MX2010008374A (es) Enriquecimiento en dos etapas de adn fetal libre de celula en plasma materno.
MX2022012594A (es) Codigos de barras flotantes.
WO2011028296A3 (en) Sequence determination by use of opposing forces
BR112022001038A2 (pt) Polinucleotídeos para a amplificação e detecção de neisseria gonorrhoeae
CN106191045B (zh) 用于多重核酸测序的Index和引物
WO2020005846A8 (en) Labeling of dna
Costa et al. Complete genome sequence of Campylobacter fetus isolated from a sheep