CN115698259A - 培养物的制造方法和细胞回收方法 - Google Patents

培养物的制造方法和细胞回收方法 Download PDF

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Publication number
CN115698259A
CN115698259A CN202280004387.0A CN202280004387A CN115698259A CN 115698259 A CN115698259 A CN 115698259A CN 202280004387 A CN202280004387 A CN 202280004387A CN 115698259 A CN115698259 A CN 115698259A
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China
Prior art keywords
soluble
culture
culture carrier
adherent cells
cells
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Pending
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CN202280004387.0A
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English (en)
Chinese (zh)
Inventor
佐藤优至
冈野定雅弘
陈尚武
高桥亮介
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Resonac Holdings Corp
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Showa Denko KK
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Publication of CN115698259A publication Critical patent/CN115698259A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2537/00—Supports and/or coatings for cell culture characterised by physical or chemical treatment
    • C12N2537/10—Cross-linking
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2539/00—Supports and/or coatings for cell culture characterised by properties
    • C12N2539/10—Coating allowing for selective detachment of cells, e.g. thermoreactive coating

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Rheumatology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202280004387.0A 2021-03-31 2022-03-29 培养物的制造方法和细胞回收方法 Pending CN115698259A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021060494 2021-03-31
JP2021-060494 2021-03-31
PCT/JP2022/015315 WO2022210659A1 (ja) 2021-03-31 2022-03-29 培養物の製造方法及び細胞回収方法

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Publication Number Publication Date
CN115698259A true CN115698259A (zh) 2023-02-03

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CN202280004387.0A Pending CN115698259A (zh) 2021-03-31 2022-03-29 培养物的制造方法和细胞回收方法

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US (1) US20230227773A1 (https=)
EP (1) EP4141106A4 (https=)
JP (1) JP7589801B2 (https=)
CN (1) CN115698259A (https=)
WO (1) WO2022210659A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117986700A (zh) * 2023-12-11 2024-05-07 国科温州研究院(温州生物材料与工程研究所) 一种微载体及制备方法与应用以及细胞贴壁培养和回收方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020150078A1 (en) * 2019-01-16 2020-07-23 Corning Incorporated Systems and methods for culturing cells in suspension
WO2020251799A1 (en) * 2019-06-13 2020-12-17 Corning Incorporated Dissolvable microcarriers for culturing cells and related methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH02171183A (ja) * 1988-12-22 1990-07-02 Agency Of Ind Science & Technol 付着性細胞の培養方法
IL300460A (en) * 2012-07-24 2023-04-01 Nissan Chemical Corp A culture medium preparation, and a method for culturing cells or tissues by using the preparation
EP3013940B1 (en) 2013-06-24 2017-04-12 Corning Incorporated Cell culture article and methods thereof
CN112662611B (zh) * 2014-01-23 2024-10-29 日产化学工业株式会社 培养基组合物
JP2018068154A (ja) * 2016-10-26 2018-05-10 旭化成メディカル株式会社 足場材料から細胞を回収する方法、及び細胞培養用の多糖類足場材料
JP7433012B2 (ja) 2019-10-07 2024-02-19 株式会社ジャパンディスプレイ 液晶表示装置

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020150078A1 (en) * 2019-01-16 2020-07-23 Corning Incorporated Systems and methods for culturing cells in suspension
WO2020251799A1 (en) * 2019-06-13 2020-12-17 Corning Incorporated Dissolvable microcarriers for culturing cells and related methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EE XIEN NG 等: "Dissolvable Gelatin-Based Microcarriers Generated through Droplet Microfluidics for Expansion and Culture of Mesenchymal Stromal Cells", BIOTECHNOL J, vol. 16, no. 3, 11 December 2020 (2020-12-11), pages 1 - 2 *
MARTIN HÄRING 等: "Protocol to Prepare Single-Cell Suspensions from Mouse Vagal Sensory Ganglia for Transcriptomic Studies", STAR PROTOC, vol. 1, no. 1, 19 June 2020 (2020-06-19), pages 7 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117986700A (zh) * 2023-12-11 2024-05-07 国科温州研究院(温州生物材料与工程研究所) 一种微载体及制备方法与应用以及细胞贴壁培养和回收方法
WO2025124086A1 (zh) * 2023-12-11 2025-06-19 国科温州研究院(温州生物材料与工程研究所) 一种微载体及制备方法与应用以及细胞贴壁培养和回收方法
CN117986700B (zh) * 2023-12-11 2026-01-06 国科温州研究院(温州生物材料与工程研究所) 一种微载体及制备方法与应用以及细胞贴壁培养和回收方法

Also Published As

Publication number Publication date
JP7589801B2 (ja) 2024-11-26
US20230227773A1 (en) 2023-07-20
EP4141106A1 (en) 2023-03-01
WO2022210659A1 (ja) 2022-10-06
JPWO2022210659A1 (https=) 2022-10-06
EP4141106A4 (en) 2023-12-20

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