CN115683964A - Biological aerosol detection instrument and method - Google Patents

Abstract

The invention relates to a biological aerosol detection instrument and a method, wherein the instrument comprises a shell (1), and an air chamber (2), an enrichment chamber (3) and an air suction chamber (4) which are sequentially arranged in the shell (1), wherein the shell (1) is provided with an air inlet hole (11), the air chamber (2) is provided with an air inlet pipe (21), the enrichment chamber (3) is provided with an enrichment hole (31), and the air suction chamber (4) is provided with an air suction pipe (20); a filter paper conveying component and a filter paper sterilizing component are also arranged in the shell (1); the filter paper conveying assembly covers the filter paper in front of the enrichment hole (31), gas enters the shell (1) through the air inlet hole (11), then enters the air chamber (2) through the air inlet pipe (21), enters the air suction chamber (4) through the enrichment hole (31) after being filtered and enriched by the filter paper in the enrichment chamber (3), and finally is discharged through the air suction pipe (20); and the filter paper conveying assembly conveys the used filter paper to the filter paper sterilizing assembly for sterilizing. Compared with the prior art, the invention can shorten the aerosol detection time, can work for many times without manual paper replacement, and avoids the risk of secondary pollution.

Description

Biological aerosol detection instrument and method

Technical Field

The invention relates to the technical field of bioaerosol detection, in particular to a bioaerosol detection instrument and a bioaerosol detection method.

Background

The biological aerosol is a gas dispersion system consisting of atmosphere and biological particles therein, has definite infectivity and sensitization, can cause diseases to enter from the nose due to long floating time and easy inhalation of aerosol particles, and the concentration and components of the aerosol affect the health of human bodies. Therefore, real-time monitoring of indoor aerosol concentration is required. At present, the real-time monitoring of the concentration of the bioaerosol still faces a lot of difficulties due to the reasons of low concentration, much interference, large span of real-time dynamic change values and the like. Bioaerosol detection mainly involves two steps of acquisition and detection, and the traditional acquisition methods can be divided into six types: (1) The impact type aerosol particle collecting device utilizes the inertia impact effect of aerosol particles, when the particles pass through the small spray holes of the round holes along with airflow with a certain flow speed, the particles with larger particle sizes can do inertia motion when having enough inertia, can leave the airflow and impact on a collecting medium to be collected. (2) The centrifugal method is that the biological aerosol particles obtain certain momentum by the centrifugal force generated by the impeller rotating at high speed in the volute and impact on the collecting medium. (3) The cyclone method is to separate aerosol particles from airflow by means of the inertia of air in the high speed cyclone of the cylindrical or conical part of the cyclone machine. (4) In the electrostatic method, aerosol particles are charged to a certain amount under the action of a high-voltage electrostatic field, so that the aerosol particles are adsorbed by a collection surface with opposite charges. (5) In the filtration method, the aerosol is retained on the filter material due to the action of the air extraction device. (6) The sedimentation method is characterized in that aerosol particles gradually settle to the surface of a collecting medium within a certain time due to the action of gravity.

The traditional bioaerosol detection method needs long-time collection and culture, and currently mainly comprises a colony unit counting method for enrichment and sampling and then culturing, a specific antibody antigen fluorescence detection method and a Polymerase Chain Reaction (PCR) method. With the development of new technologies, fluorescence detection, gas chromatography, and numerical analysis are gradually replacing the conventional methods for bioaerosol detection. The fluorescence detection method is a biological aerosol rapid detection technology, and the principle is that organic molecules which represent biological activity, such as phenylalanine, tyrosine, tryptophan, nicotinamide adenine dinucleotide, riboflavin and the like in biological particles can generate intrinsic fluorescence under the induction of ultraviolet light, and whether the biological particles are contained in the aerosol can be distinguished by the method. The bioaerosol detection instrument based on the fluorescence detection method is characterized in that an air pump is used for collecting an air sample, and the sample is irradiated by ultraviolet light with specific wavelength and then excited fluorescence intensity detected by a sensor, so that the concentration of biological particles contained in the aerosol is obtained.

A novel biological aerosol coronavirus nucleic acid monitoring system of Hangzhou catalpine biotechnology limited company adopts a biological aerosol collector to directly collect new coronary coronavirus particles in air into a collecting pipe matched with a detection chip. After the detection chip is placed in a pathogen nucleic acid analyzer, the extraction, amplification and analysis of nucleic acid can be automatically completed. The instrument is bulky and requires manual lofting. The aerosol monitor 8532 from TSI corporation of tessela, usa is a hand-held battery-powered light-scattering laser photometer with data recording function, which uses a sheath gas system to separate the aerosol in the optical element chamber, so that the optical element can be kept clean, the reliability of the device is improved, but only the particle concentration can be detected and whether the particle is a bioaerosol or not can not be distinguished. A BS-Z01 portable air virus detector of Beijing Bishuijing essence instrument science and technology Limited company is a portable, quick and efficient instrument for quickly screening environmental air samples, adopts an immune antigen detection technology, and has a long single detection time of 15min. Other related products also have the defects of large volume, complex structure, manual intervention in the detection process and the like.

Disclosure of Invention

The invention aims to provide a bioaerosol detection instrument and method, which can work for multiple times without manual paper replacement, shorten the aerosol detection time and avoid secondary pollution risk.

The purpose of the invention can be realized by the following technical scheme: a bioaerosol detecting instrument comprises a shell, and an air chamber, an enrichment chamber and an air suction chamber which are sequentially arranged in the shell, wherein the shell is provided with an air inlet hole; a filter paper conveying assembly and a filter paper sterilizing assembly are also arranged in the shell;

the filter paper conveying assembly covers the filter paper in front of the enrichment hole, gas enters the shell through the gas inlet hole, enters the gas chamber through the gas inlet pipe, enters the air suction chamber through the enrichment hole after being filtered and enriched by the filter paper in the enrichment chamber, and is finally discharged through the air suction pipe; and the filter paper conveying assembly conveys the used filter paper to the filter paper sterilizing assembly for sterilizing.

Preferably, the filter paper conveying assembly comprises a filter paper holder for placing unused filter paper, a paper feed motor for driving the filter paper holder to rotate, a wastepaper holder for placing used filter paper and a wastepaper bin motor for driving the wastepaper holder to rotate; the filter paper on the filter paper rack enters the enrichment chamber from the paper inlet, the enrichment hole is covered for filtration and enrichment, and the used filter paper extends out from the paper outlet and is wound on the waste paper rack.

Further preferably, a paper inlet right-angle bevel gear is arranged between the filter paper frame and the paper inlet motor, and a waste paper bin right-angle bevel gear is arranged between the waste paper frame and the waste paper bin motor.

Preferably, the filter paper sterilizing assembly comprises a waste paper bin and an ultraviolet sterilizing light source arranged in the waste paper bin, and the filter paper conveying assembly conveys the used filter paper into the waste paper bin for ultraviolet sterilizing.

Preferably, an ultraviolet excitation light source and a fluorescence detector are arranged in the gas chamber, and the ultraviolet excitation light source and the fluorescence detector are arranged on the same wall surface of the gas chamber.

Further preferably, the ultraviolet excitation light source is annularly arranged outside the fluorescence detector.

Further preferably, the shell in still be provided with circuit storehouse and display screen, be equipped with signal acquisition module, signal processing module and data storage module in the circuit storehouse, signal acquisition module control fluorescence sensor acquisition signal, the signal processing module of the signal acquisition of fluorescence detector is handled as biological aerosol concentration information, stores in data storage module to show on the display screen.

Still further preferably, a power supply module and a control module are further arranged in the circuit bin.

Preferably, the air suction chamber is connected with an air pump through an air suction pipe, and the air pump discharges air through an air discharge pipe and an air outlet hole arranged on the shell.

Preferably, the air inlet pipe is a right-angle elbow, light absorbing materials are coated in the pipeline of the right-angle elbow and the inner wall of the air chamber, and the shell and the air chamber are made of light-tight materials.

The use method of the bioaerosol detection instrument comprises the following steps:

s1: starting a paper inlet motor and a waste paper bin motor, and conveying and covering filter paper in front of the enrichment hole;

s2: starting the air pump, allowing external air to enter the shell from the air inlet, allowing the external air to enter the air chamber through the air inlet pipe, filtering and enriching the external air through filter paper in the enrichment chamber, allowing the external air to enter the air suction chamber through the enrichment hole, and finally allowing the external air to be discharged from the air outlet through the air suction pipe;

s3: turning off the air pump, and starting the ultraviolet excitation light source and the fluorescence detector to perform ultraviolet excitation and fluorescence detection on biological particles on the filter paper, wherein in the detection process, the ultraviolet killing light source is always in an open state to perform ultraviolet killing on the used filter paper and other parts of the waste paper bin;

s4: the signal collected by the fluorescence detector is processed into bioaerosol concentration information by the signal processing module, stored in the data storage module and displayed on the display screen;

s5: and closing the ultraviolet excitation light source and the fluorescence detector, and entering the next detection process.

The traditional bioaerosol detection needs steps such as collection, culture and the like, the culture process has higher requirements on the environment, and the detection process adopts an immune antigen or a polymerase chain reaction technology, so that the single detection time is longer; in order to improve the sensitivity and reduce the environmental interference, a complex light path, a high-power ultraviolet excitation light source, a complex air chamber and a particle collection structure are adopted, so that the volume and the instability of the instrument are greatly increased; reaction solution, filter paper or chromatography test paper strip need to be frequently replaced, automation is low, and manual intervention is needed in the detection process; the waste liquid and waste material produced by the instrument need to be treated separately, and the risk of secondary pollution is generated in the transportation process. The invention can shorten the detection time, solve the problem of longer detection time of aerosol detection in a single time, and reduce the volume of the instrument by optimizing the acquisition and light path structure of the aerosol detection instrument; an automatic aerosol collecting structure is designed, and manual intervention is not needed in the working process of the instrument; the disinfection and sterilization device is designed to disinfect and kill the used enrichment filter paper, so that secondary pollution is prevented.

Compared with the prior art, the invention has the following advantages:

1. the invention shortens the aerosol detection time, reduces the instrument volume, avoids the secondary pollution risk and realizes the automatic detection of the biological aerosol;

2. according to the invention, the air pump is adopted for pumping air, and the filter paper is adopted for enriching biological particles, so that the detection time is shortened, and the problem of long detection time of aerosol detection in a single time is solved;

3. according to the invention, an ultraviolet excitation light source and a fluorescence detector are arranged on the same side, the acquisition and light path structure of the aerosol detection instrument is optimized, and the volume of the instrument is reduced;

4. the invention adopts the motor to control the rotation of the filter paper cylinder and the waste paper cylinder to realize the automatic replacement of the enriched filter paper, and the instrument can work for many times without manual paper replacement;

5. the invention designs a disinfection and sterilization device, a waste paper bin is provided with a high-power ultraviolet lamp with the wavelength of 222nm, and used enrichment filter paper and the waste paper bin are disinfected and killed to prevent secondary pollution;

6. according to the invention, through the coating of the light absorption material and the design of the light-tight material, ambient light can be prevented from entering the air chamber to interfere the detection result, and the detection precision of the instrument is improved;

7. the invention integrates all components in the shell, has compact structure and high integration level and is more portable.

Drawings

FIG. 1 is a graph of the fluorescence spectrum of biological particles excited under 266nm ultraviolet light;

FIG. 2 is a side sectional view of a bioaerosol testing instrument of the present invention;

FIG. 3 is a view showing the structure of the housing of the bioaerosol detecting apparatus according to the present invention;

FIG. 4 is a schematic diagram of the circuit module of the bioaerosol detecting instrument of the present invention;

FIG. 5 is a schematic view showing the internal structure of a waste paper bin of the bioaerosol detecting apparatus according to the present invention;

FIG. 6 is a schematic view of the internal structure of the air pump chamber of the bioaerosol detecting apparatus of the present invention;

FIG. 7 is a schematic view of a gas cell sensor of the bioaerosol detecting apparatus according to the present invention;

FIG. 8 is a schematic diagram of the structure of the enrichment chamber of the bioaerosol detecting apparatus of the present invention;

in the figure: 1-shell, 11-air inlet hole, 2-air chamber, 21-air inlet pipe, 3-enrichment chamber, 31-enrichment hole, 4-air suction chamber, 5-paper filter holder, 6-paper inlet motor, 7-paper waste holder, 8-paper waste bin motor, 9-paper inlet, 10-paper outlet, 12-paper inlet right angle bevel gear, 13-paper waste bin right angle bevel gear, 14-paper waste bin, 15-ultraviolet disinfection light source, 16-ultraviolet excitation light source, 17-fluorescence detector, 18-circuit bin, 181-signal acquisition module, 182-signal processing module, 183-data storage module, 184-power supply module, 185-control module, 19-display screen, 20-air suction pipe, 22-air pump, 23-air exhaust pipe, 24-air outlet hole, 25-fixed screw hole and 26-USB communication charging hole.

Detailed Description

The invention is described in detail below with reference to the figures and specific embodiments. The following examples are carried out on the premise of the technical scheme of the invention, and detailed embodiments and specific operation processes are given, but the scope of the invention is not limited to the following examples.

Example 1

A portable bioaerosol detecting instrument comprises a shell 1, an air chamber 2, an enrichment chamber 3, an air suction chamber 4, a filter paper conveying assembly and a filter paper sterilizing assembly, wherein the shell 1 is provided with an air inlet 11, the air chamber 2 is provided with an air inlet pipe 21, the air chamber 2 is internally provided with an ultraviolet excitation light source 16 and a fluorescence detector 17, the enrichment chamber 3 is provided with an enrichment hole 31, the air suction chamber 4 is communicated with an air pump 22, the filter paper conveying assembly conveys and covers the filter paper to the front of the enrichment hole 31, the air enters the shell 1 through the air inlet 11, then enters the air chamber 2 through the air inlet pipe 21, is filtered and enriched through the filter paper in the enrichment chamber 3, enters the air suction chamber 4 through the enrichment hole 31, and finally is discharged through the air suction pipe 20 and the air pump 22; and the filter paper conveying assembly conveys the used filter paper to the filter paper sterilizing assembly for sterilizing. In the gas chamber 2, the ultraviolet excitation light source 16 irradiates the bioaerosol to excite a fluorescence signal, and the fluorescence detector 17 converts the fluorescence signal into an electric signal.

Example 2

A portable bioaerosol detecting instrument is shown in figures 2-8 and comprises a shell 1, an air chamber 2, a waste paper processing bin (a waste paper bin 14), an air pump chamber and a circuit battery bin (a circuit bin 18), wherein the shell 1 comprises a front touch display screen 19, a touch switch is arranged on the lower portion of the touch display screen 19, three groups of air inlet holes 11 are formed in the side face and the upper face of the shell 1, the left side face is fixedly connected with the shell 1 through four fixing screw holes 25, the instrument comprises an air outlet hole 24 at the lower left side and a USB communication charging hole 26 at the lower right side. The gas chamber 2 comprises three groups of gas inlet pipes 21 on the top and the side, a fluorescence detector 17 and a group of ultraviolet (266 nm) LEDs (ultraviolet excitation light sources 16) are arranged on one side wall, the ultraviolet light with the wavelength of 266nm irradiates the bioaerosol to excite a fluorescence signal, and the fluorescence detector 17 converts the fluorescence signal into an electric signal. The other side wall is provided with a group of enrichment holes 31, a baffle plate with holes is arranged in front of the enrichment holes 31 and used for fixing filter paper, an air suction chamber 4 is arranged behind the enrichment holes 31, the air suction chamber 4 is connected with an air suction pipe 20 in the air pump chamber, a paper inlet 9 is arranged above the enrichment chamber 3, a paper outlet 10 is arranged below the enrichment chamber 3, and the filter paper enters the enrichment chamber 3 from the paper inlet 9 and then enters the waste paper processing bin from the paper outlet 10. The waste paper treatment bin comprises a paper outlet 10, a waste paper bin motor 8, a waste paper frame 7 and a waste paper bin right-angle bevel gear 13, when a group of data is measured, the waste paper bin motor 8 is started, the waste paper frame 7 is driven to rotate through the waste paper bin right-angle bevel gear 13, filter paper is rolled on the waste paper frame 7, and the function of automatically replacing and enriching filter paper is achieved. A group of ultraviolet LEDs (ultraviolet light source 15) with the wavelength of 222nm is arranged below the paper outlet 10, and after the ultraviolet LEDs are opened, ultraviolet sterilization can be carried out on the filter paper and the waste paper bin, so that the self-cleaning function of the instrument is realized. The air pump room includes air pump 22, breathing pipe 20 and blast pipe 23 that link to each other with air pump 22, when the air pump is opened, breathing pipe 20 extracts air chamber 2 is gaseous, discharge through blast pipe 23, this process is the enrichment, can be with the biological particle enrichment in the aerosol on filter paper, paper feed mouth motor 6 is settled to a gas pump room lateral wall, link to each other with filter paper frame 5 through paper feed mouth right angle bevel gear 12, when the motor starts, drive filter paper frame 5 through paper feed mouth right angle bevel gear 12 and rotate, realize changing the enrichment filter paper function, filter paper frame 5 all can be dismantled with waste paper frame 7, conveniently change the filter paper section of thick bamboo. The circuit battery compartment comprises an instrument battery and a circuit board part, the circuit board further comprises a power supply circuit (a power supply module 184), a system control circuit (a control module 185), a signal acquisition circuit (a signal acquisition module 181), a signal processing circuit (a signal processing module 182), and a data storage display circuit (a data storage module 183), the power supply circuit controls the battery to charge and the instrument to supply power, the system control circuit controls the operation of each part of the instrument, the system control circuit comprises an air pump 22, a fluorescence detector 17, an ultraviolet (266 nm) LED, an ultraviolet (222 nm) LED, a waste paper bin motor 8, a switch of a paper inlet motor 6 and the operation of each part of the circuit, the signal acquisition circuit controls the fluorescence sensor to acquire signals, the signal processing circuit calculates and processes the signals acquired by the fluorescence sensor to obtain bioaerosol concentration information, and the data storage display circuit stores the signals acquired by the fluorescence sensor and the bioaerosol concentration information and displays the bioaerosol concentration information through a display screen 19.

The instrument detection principle of the invention is that bioactive organic molecules such as phenylalanine, tyrosine, tryptophan, nicotinamide adenine dinucleotide and riboflavin in biological particles can generate intrinsic fluorescence under the induction of ultraviolet light, and the fluorescence spectrum of the biological particles under the excitation of 266nm ultraviolet light is shown in figure 1. The concentration information of the biological particles in the aerosol can be obtained by detecting the fluorescence intensity through a photoelectric sensor (fluorescence detector).

After the instrument is started, the display screen 19 is opened, detection is selected to start, the paper inlet motor 6 and the waste paper bin motor 8 work to cover filter paper in front of the enrichment hole 31, then the air pump 22 starts to work to enrich for 1min, air enters the shell 1 from the air inlet hole 11, enters the air chamber 2 through the air inlet pipe 21, is filtered and enriched through the filter paper in the enrichment chamber 3, enters the air suction chamber 4 through the enrichment hole 31, and is discharged through the air suction pipe 20, the exhaust pipe 23 and the air outlet hole 24. After the enrichment is completed, the air pump 22 is closed, the ultraviolet (266 nm) LED (ultraviolet excitation light source 16) and the fluorescence detector 17 are turned on to perform ultraviolet excitation and fluorescence detection on the biological particles on the filter paper, and the signals and the biological aerosol concentration information acquired by the fluorescence sensor are stored and displayed through the signal acquisition module 181, the signal processing module 182, the data storage module 183, the touch screen switch and the display module (display screen). The next step is to turn off the UV (266 nm) LED and fluorescence detector 17 for the next detection run. In the detection process, an ultraviolet (222 nm) LED (ultraviolet sterilization light source 15) is always in an open state to perform ultraviolet sterilization on waste paper and other parts of a waste paper bin. The time for one detection process is about 1.5 min.

Example 3

A portable bioaerosol detecting instrument is characterized in that an air inlet pipe 21 is designed as a right-angle elbow, light absorbing materials are coated on the inner wall of a pipeline and the inner wall of an air chamber, and light-tight materials are adopted by an inner shell and an outer shell to prevent ambient light from entering the air chamber to interfere with a detection result. The rest is the same as in example 2.

The embodiments described above are described to facilitate an understanding and use of the invention by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above embodiments, and those skilled in the art should make improvements and modifications within the scope of the present invention based on the disclosure of the present invention.

Claims (10)

1. A bioaerosol detecting instrument is characterized by comprising a shell (1), and an air chamber (2), an enrichment chamber (3) and an air suction chamber (4) which are sequentially arranged in the shell (1), wherein an air inlet hole (11) is formed in the shell (1), an air inlet pipe (21) is arranged on the air chamber (2), an enrichment hole (31) is formed in the enrichment chamber (3), and an air suction pipe (20) is arranged on the air suction chamber (4); a filter paper conveying component and a filter paper sterilizing component are also arranged in the shell (1);

the filter paper conveying assembly covers the filter paper in front of the enrichment hole (31), gas enters the shell (1) through the air inlet hole (11), then enters the air chamber (2) through the air inlet pipe (21), enters the air suction chamber (4) through the enrichment hole (31) after being filtered and enriched by the filter paper in the enrichment chamber (3), and finally is discharged through the air suction pipe (20); and the filter paper conveying assembly conveys the used filter paper to the filter paper sterilizing assembly for sterilizing.

2. The bioaerosol detecting instrument as set forth in claim 1, wherein the filter paper feeding assembly comprises a filter paper holder (5) for holding unused filter paper, a paper feed motor (6) for driving the filter paper holder (5) to rotate, a wastepaper holder (7) for holding used filter paper, and a wastepaper bin motor (8) for driving the wastepaper holder (7) to rotate; the filter paper on the filter paper frame (5) enters the enrichment chamber (3) from the paper inlet (9), the enrichment hole (31) is covered for filtration, and the used filter paper extends out from the paper outlet (10) and is wound on the waste paper frame (7).

3. The bioaerosol detecting instrument as claimed in claim 2, wherein a paper feed right angle bevel gear (12) is arranged between the filter paper holder (5) and the paper feed motor (6), and a waste paper bin right angle bevel gear (13) is arranged between the waste paper holder (7) and the waste paper bin motor (8).

4. The bioaerosol testing instrument of claim 1, wherein the filter paper sterilizing assembly comprises a waste paper bin (14) and an ultraviolet sterilizing light source (15) disposed in the waste paper bin (14), and the filter paper conveying assembly conveys the used filter paper into the waste paper bin (14) for ultraviolet sterilization.

5. The bioaerosol detecting instrument as claimed in claim 1, wherein the gas chamber (2) is provided with an ultraviolet excitation light source (16) and a fluorescence detector (17), and the ultraviolet excitation light source (16) and the fluorescence detector (17) are arranged on the same wall surface of the gas chamber (2).

6. The bioaerosol detecting instrument as claimed in claim 5, wherein the housing (1) is further provided with a circuit chamber (18) and a display screen (19), the circuit chamber (18) is provided with a signal acquisition module (181), a signal processing module (182) and a data storage module (183), the signal acquisition module (181) controls the fluorescence sensor (17) to acquire signals, the signals acquired by the fluorescence detector (17) are processed by the signal processing module (182) to be bioaerosol concentration information, and the bioaerosol concentration information is stored in the data storage module (183) and displayed on the display screen (19).

7. The bioaerosol testing instrument of claim 6, wherein a power supply module (184) and a control module (185) are further disposed in the circuit chamber (18).

8. The bioaerosol testing instrument of claim 1, wherein the air suction chamber (4) is connected to an air pump (22) through an air suction tube (20), and the air pump (22) discharges air through an air discharge tube (23) and an air outlet (24) provided on the housing (1).

9. The bioaerosol detecting instrument as claimed in claim 1, wherein the air inlet tube (21) is a right-angle elbow, light absorbing materials are coated inside the pipeline of the right-angle elbow and the inner wall of the air chamber (2), and light-proof materials are adopted for the housing (1) and the air chamber (2).

10. A bioaerosol assay method carried out using the apparatus of any one of claims 1 to 9, comprising the steps of:

s1: starting a paper inlet motor (6) and a waste paper bin motor (8), and conveying and covering filter paper to the front of the enrichment hole (31);

s2: starting the air pump (22), introducing outside air into the shell (1) from the air inlet hole (11), introducing the outside air into the air chamber (2) through the air inlet pipe (21), filtering and enriching the outside air through filter paper in the enrichment chamber (3), introducing the outside air into the air suction chamber (4) through the enrichment hole (31), and finally discharging the outside air from the air outlet hole (24) through the air suction pipe (20);

s3: turning off the air pump (22), starting an ultraviolet excitation light source (16) and a fluorescence detector (17) to carry out ultraviolet excitation and fluorescence detection on biological particles on the filter paper, wherein in the detection process, an ultraviolet killing light source (15) is always in an open state to carry out ultraviolet killing on the used filter paper and other parts of the waste paper bin (14);

s4: the signal collected by the fluorescence detector (17) is processed into bioaerosol concentration information by a signal processing module (181), stored in a data storage module (182) and displayed on a display screen (19);

s5: and (3) closing the ultraviolet excitation light source (16) and the fluorescence detector (17) and entering the next detection process.

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