CN109303623B - A kind of animal exposure experimental provision - Google Patents
A kind of animal exposure experimental provision Download PDFInfo
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- CN109303623B CN109303623B CN201811275872.3A CN201811275872A CN109303623B CN 109303623 B CN109303623 B CN 109303623B CN 201811275872 A CN201811275872 A CN 201811275872A CN 109303623 B CN109303623 B CN 109303623B
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- cleaning box
- case
- pollution
- pollution case
- air inlet
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Abstract
The present invention provides a kind of animal exposure experimental provision, including polluting case, cleaning box, aspiration pump, blowdown valve, collector, cutting head, high efficiency particulate air filter, air inlet pipe, exhaust tube, the pollution case and cleaning box pass through air inlet pipe and exhaust tube and connect with the external world, the pollution case and cleaning box pass through exhaust tube and connect with aspiration pump, the pollution case and cleaning box bottom are equipped with blowdown valve, the air inlet pipe of the pollution case is equipped with cutting head, collector is equipped between pollution case and the aspiration pump, the air inlet pipe of the cleaning box is equipped with high efficiency particulate air filter.Animal exposure experimental provision provided by the invention, overall structure is simple, easy to operate, and the gaseous pollutant concentration level polluted in case and cleaning box is consistent with the external world, the actual conditions for more meeting present air pollution have certain reference value to the measurement of present Chinese air quality.
Description
Technical field
The present invention relates to a kind of experimental provision more particularly to a kind of animal exposure experimental provisions.
Background technique
Currently, the environmental pollution of China is increasingly severe, air quality worse and worse, especially Shijiazhuang, Beijing, Hebei
Equal north cities, the crowd to live herein generally persistently live under such circumstances, and exposure duration is long, although atmosphere pollution
Concentration is lower, but the health of low concentration exposure on human long-term in this way has very big threat.Therefore, many scholars start to use
The method of Animal Inhalation Toxicity Exposure carrys out harm of the simulated atmosphere pollutant to human body.
Chinese utility model patent (201320491757.6: animal systemic exposure room) discloses a kind of animal systemic exposure
Room, which is characterized in that including interlude circular cylindrical cavity and upper conical cavity and lower conical cavity；The upper end
It is provided at the top of conical bores aerosol import (10), aerosol import (10) connection atomization diffuser (20), institute
State the aerosol that atomization diffuser (20) is used to diffuse into animal systemic exposure room.Though the animal systemic exposure room
So make being more evenly distributed for internal aerosol, the concentration of aerosol is more stable, but the animal systemic exposure room cannot access other
Gaseous pollutant, and gaseous pollutant cannot be diluted, while only pollution case, observation allogenic animal that cannot be straightforward exist
The differential responses for polluting case and cleaning box, thus the concentration pair of which kind of gaseous pollutant of comparative analysis and gaseous pollutant
The harm of animal.
Chinese invention patent (201310351071.1: more concentration animal mouth and nose suck dynamic exposure unit) discloses one kind
More concentration animal mouth and nose suck dynamic exposure unit, including experimental group exposure chamber (1), which is characterized in that the experimental group exposure
Room (1) includes first segment exposure chamber (11) and the second section exposure chamber (12)；In the first segment exposure chamber (11) and described second
It is additionally provided between section exposure chamber (12) and is entered in the second section exposure chamber (12) to by the first segment exposure chamber (11)
The aerosol processing unit that is diluted of aerosol: it is sudden and violent that the aerosol processing unit is also used to make to enter second section
Reveal uniform concentration distribution of the aerosol in room (12) in the second section exposure chamber (12).Although the exposure unit can be
The zoopery and comparative analysis of the more concentration of aerosol are carried out in same device, but the gaseous pollutant of the device to test is single,
And structure is complicated, it is cumbersome.
Chinese invention patent (201710029278.5: the animal imbedibility exposure system for PM2.5) discloses one kind
Animal imbedibility exposure system for PM2.5, which is characterized in that including air generation plants, aerosol generator, flow
Valve, PM2.5 exposure compartment, PM2.5 monitor system, PM2.5 filtration system, wherein the air generation plants, the gas are molten
Glue generator, the flow valve, exposure compartment described in the PM2.5, the PM2.5 monitor system, the PM2.5
Filtration system is sequentially connected.The animal imbedibility experiment of the exposure system causes the physico property of gaseous pollutant to change, because
This, cannot accurately measure influence of the gaseous pollutant to animal.
Summary of the invention
To solve problems of the prior art, the present invention provides a kind of animal exposure experimental provision, structure is simple,
It is easy to operate, control exposure, and the gaseous pollutant in two exposure casees can be carried out under a variety of gaseous contamination substance environments to animal
Concentration level is consistent with the external world.The actual conditions for more meeting present air pollution have the measurement of Chinese air quality certain
Guiding value.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of animal exposure experimental provision, including dirt
Contaminate case, cleaning box, aspiration pump, blowdown valve, collector, cutting head, high efficiency particulate air filter, air inlet pipe, exhaust tube, the pollution case with
Cleaning box passes through air inlet pipe and exhaust tube and connect with the external world, and the pollution case and cleaning box pass through exhaust tube and aspiration pump company
It connects, tiny structure is formed by aspiration pump, outside air is introduced into cabinet；The pollution case and cleaning box bottom are equipped with blowdown
Valve, for cleaning pollution case and cleaning box blowdown, the air inlet pipe of the pollution case is equipped with cutting head, the pollution case and pumping
Collector is equipped between pump, the air inlet pipe of the cleaning box is equipped with high efficiency particulate air filter；
The pollution case and cleaning box include interlude square cavity and upper conical cavity and lower end circular cone
Shape cavity；
It is additionally provided with an air inlet on the pollution case interlude square cavity, zero Dilution air can be accessed if necessary
Case endoparticle object concentration achievees the purpose that control exposure；
It is described pollution case and cleaning box interlude square cavity two sides be uniformly provided with 4 air distribution ports, can be by connecing
Enter the gaseous pollutant of a variety of various concentrations, carries out gaseous hydrocarbon yield exposure.
Animal exposure experimental provision using the above structure, it is different since pollution case and cleaning box body are completely the same
Part is the device in external gas circuit and gas circuit.Pollution case and cleaning box are tested simultaneously, and experimental efficiency is high, and the time is short,
Cleaning box as a control group, can be straightforward observation allogenic animal pollution case and cleaning box differential responses, thus than
Harm compared with the concentration of which kind of gaseous pollutant of analysis and gaseous pollutant to animal；The experiment dress of animal exposure simultaneously
It sets, overall structure is simple, and easy to operate, the gaseous pollutant concentration level polluted in case and cleaning box is consistent with the external world, more accords with
The actual conditions for closing present air pollution have certain reference value to the measurement of present Chinese air quality.
Preferably, the pollution case and cleaning box material are polishing stainless steel.
Preferably, gaseous pollutant PM2.5, PM10, SO2、NO2、O3, CO, but it is not limited to this, can also be other gas
State pollutant.
Preferably, several locking devices are additionally provided on the interlude square cavity of the pollution case and cleaning box,
For locking exposed chamber door.
Preferably, the cutting head is detachable, replacement, according to different requirement of experiment, selection be equipped with PM2.5, PM10,
One of PM1, or cutting head is not set, keep the gas for entering pollution case and the external world completely the same.
Preferably, particle sampling film is equipped in the collector, the particle sampling film is for collecting in exposed case
Actual particulate matter, and by various chemical analysis means, determine the chemical constituent of the particulate matter of actual exposed in case.
Preferably, the high efficiency particulate air filter is stage high-effective filter, in the stage high-effective filter from top to bottom according to
The secondary level vehicle that is equipped with is crossed with filter film, second level high efficiency filter film, stage high-effective filter membrane；
Preferably, less than 1 micron, the stage high-effective filter membrane is that HEPA is efficient in the aperture of the second level high efficiency filter film
Filter membrane, the aperture of the HEPA high efficiency filter film is less than 0.3 micron, it is ensured that enters the removal of the PM2.5 in case to cleaning box
Rate is greater than 97.5%.
Preferably, the pollution case and cleaning box are equipped with cylindrical seal item, described for guaranteeing the leakproofness of cabinet
It is also equipped with glass vision panel on pollution chamber door and cleaning chamber door, is read for observing animal situation and automatic monitored control system in case
Number.
Preferably, it is equipped with support frame on the pollution case and cleaning box, support frame as described above low side is equipped with 4 idler wheels, institute
Idler wheel is stated equipped with brake gear, can be fixed after mounting.
Preferably, portable online PM2.5 detection device, humidity sensor are additionally provided in the pollution case and cleaning box
Device, long-range monitoring camera, automatic monitored control system, gas concentration sensor.
Preferably, the automatic monitored control system includes illumination self-con-tained unit, electronic temperature controller.
Preferably, 22 ± 2 DEG C of the temperature inside the box are controlled, while passing through lamp with auto-switch device, the light and shade of control cabinet intraoral illumination
Time.
Preferably, the gas concentration sensor can detect the concentration of PM2.5, PM10, SO2, NO2, O3, CO.
It compares with the prior art, the beneficial effect comprise that:
(1) pollution case and cleaning box provided by the invention are tested simultaneously, and experimental efficiency is high, and the time is short, and cleaning box is made
For control group, observation allogenic animal that can be straightforward is in the differential responses and growth and active state for polluting case and cleaning box
Deng thus harm of the concentration of which kind of gaseous pollutant of comparative analysis and gaseous pollutant to animal；
(2) exposure chamber provided by the invention is connect only by air inlet pipe and exhaust tube with the external world, is guaranteed in cleaning box
PM2.5 concentration is lower than 20 μ g/m3, and pollution case is consistent with the external world.Pollute gaseous pollutant concentration level in case and cleaning box with
It is extraneous consistent, more meet the actual conditions of present air pollution, has certain reference price to the measurement of present Chinese air quality
Value；
(3) present invention provides animal exposure experimental provision and avoids traditional animal nasal cavity or tracheal instillation experiment bring
The problem of animal injury and change particulate matter physicochemical property；
(4) 8 air distribution ports are equipped on pollution case and clean box body provided by the invention, it can be dense by accessing a variety of differences
Zero air of gaseous pollutant or supplement of degree, carries out gaseous hydrocarbon yield exposure, meets the requirement of experiment, be also convenient in difference
The comparison tested under concentration difference gaseous pollutant.
(5) animal exposure experimental provision structure provided by the invention is simple, easy to operate.
(6) it can guarantee in the present invention using high efficiency particulate air filter and the PM2.5 removal rate that cleaning box enters in case be greater than
97.5%, it can satisfy the requirement of experiment.
Detailed description of the invention
Fig. 1 is a kind of animal experiment device structural schematic diagram provided by the invention；
Fig. 2 is the front elevation of pollution case and cleaning box provided by the invention；
Fig. 3 is pollution case side view provided by the invention.
Attached concrete meaning marked in the figure is as follows:
1: pollution case；2: cleaning box；3: cutting head；4: air inlet；5: collector；6: aspiration pump；7: blowdown valve；8: automatic
Monitoring system；9: high efficiency particulate air filter；10: air inlet pipe；11: idler wheel；12: locking device；13: glass vision panel；14: air distribution port.
Specific embodiment
With reference to the accompanying drawing, a specific embodiment of the invention is described in detail.
Join shown in Fig. 1-3, Fig. 1 is animal experiment device structural schematic diagram provided by the invention；Fig. 2 is provided by the invention
Pollute the front elevation of case and cleaning box；Fig. 3 is the side view of pollution case provided by the invention.Zoopery dress provided by the invention
Set structural schematic diagram, a kind of animal exposure experimental provision, including pollution case 1, cleaning box 2, aspiration pump 6, blowdown valve 7, collector
5, cutting head 3, high efficiency particulate air filter 9, air inlet pipe 10, exhaust tube pollute case 1 and cleaning box 2 and pass through air inlet pipe 10 and exhaust tube
It is connect with the external world, pollutes case 1 and cleaning box 2 and connect by exhaust tube with aspiration pump 6, polluted case 1 and 2 bottom of cleaning box is all provided with
There is blowdown valve 7, the air inlet pipe 10 of pollution case 1 is equipped with cutting head 3, and cutting head 3 is PM2.5 cutting head in the present embodiment, but not
It is limited to this, cutting head 3 is detachable, replacement also may be selected to be equipped with one of PM10, PM1 according to different requirement of experiment, or
Person does not set cutting head 3, keeps the gas for entering cabinet and the external world completely the same, more meets the actual conditions of present air pollution, right
The measurement of China's air quality has certain reference value now；External environment can accurately be more tested to human or animal's simultaneously
It influences.
It pollutes and is equipped with collector 5 between case 1 and aspiration pump 6, collector 5 includes particle sampling film, particle sampling film
For collecting actual particulate matter in pollution case 1, and by various chemical analysis means, actual exposed in pollution case 1 is determined
The chemical constituent of particulate matter.Wherein, particulate matter can be PM2.5 sampling film.The air inlet pipe 10 of cleaning box 2 is equipped with high efficiency particulate air filter
9, high efficiency particulate air filter 9 is stage high-effective filter, is successively arranged level vehicle filtering in stage high-effective filter from top to bottom
Film, second level high efficiency filter film, stage high-effective filter membrane；The aperture of second level high efficiency filter film is less than 1 micron, stage high-effective filter membrane
For HEPA high efficiency filter film, the aperture of HEPA high efficiency filter film is less than 0.3 micron, it is ensured that enters in case to cleaning box
PM2.5 removal rate is greater than 97.5%.
The material for polluting case 1 and cleaning box 2 is polishing stainless steel, pollutes case 1 and cleaning box 2 includes interlude pros body cavity
Body and upper conical cavity and lower conical cavity, wherein pollution case 1 interlude square cavity be equipped with one into
Port 4 can access zero Dilution air case endoparticle object concentration if necessary, achieve the purpose that control exposure.
Wherein, it pollutes case 1 and the interlude square cavity two sides of cleaning box 2 is uniformly provided with 4 air distribution ports 14, can lead to
The gaseous pollutant for accessing a variety of various concentrations is crossed, gaseous hydrocarbon yield exposure is carried out.Gaseous pollutant be PM2.5, PM10,
SO2、NO2、O3, CO, but it is not limited to this, can also be other gaseous pollutants.In the present embodiment, case 1 and cleaning box 2 are polluted
Interlude square cavity on be additionally provided with 2 locking devices 12, but not limited to this, locking device 12 is for locking exposure
Chamber door.
In addition, pollution case 1 and cleaning box 2 are equipped with cylindrical seal item, for guaranteeing the leakproofness of cabinet, case is polluted
1 and cleaning box 2 on be also equipped with glass vision panel 13, for observing in case animal situation and automatic monitored control system reading.It is dirty
Support frame is equipped on dye case 1 and cleaning box 2, support frame as described above low side is equipped with 4 idler wheels 11, and brake dress is additionally provided on idler wheel 11
It sets, for controlling pollution case 1 and cleaning box 2；
Portable online PM2.5 detection device, humidity sensor, long-range monitoring are additionally provided in pollution case 1 and cleaning box 2
Camera, automatic monitored control system 8, gas concentration sensor.Wherein, automatic monitored control system 8 includes illuminating self-con-tained unit, temperature certainly
Control device.Electronic temperature controller controls the temperature inside the box within the scope of 22 ± 2 DEG C, while passing through lamp with auto-switch device, control
The light and shade time of case intraoral illumination.
Gas concentration sensor can detect PM2.5, PM10, SO2、NO2、O3, CO concentration.
Animal exposure experimental provision provided by the invention, structure is simple, easy to operate, can be to animal in a variety of gaseous contaminations
Control exposure is carried out under substance environment, and the gaseous pollutant concentration level polluted in case 1 and cleaning box 2 is consistent with the external world.Pollution
The cabinet of case 1 is equipped with air inlet 4, for supplementing zero air, to guarantee experiment demand, is passed through zero air when necessary and is diluted
It adjusts；Pollution case 1 and cleaning box 2 simultaneously tested, experimental efficiency is high, and the time is short, cleaning box 2 as a control group, Ke Yizhi
Clear observation allogenic animal is connect in the differential responses of pollution case 1 and cleaning box 2, thus which kind of gaseous pollutant of comparative analysis with
And harm of the concentration of gaseous pollutant to animal.
A series of detailed description illustrating only for feasible embodiment of the invention described above,
They are not to limit the scope of the invention, in the knowledge that the technical field those of ordinary skill has
Interior, the various change made without departing from the purpose of the present invention all belongs to the scope of protection of the present invention.
Claims (4)
1. a kind of animal exposure experimental provision, including pollution case, cleaning box, aspiration pump, blowdown valve, collector, cutting head, efficiently
Filter, air inlet pipe, exhaust tube, which is characterized in that the pollution case and cleaning box pass through air inlet pipe and exhaust tube and the external world
Connection, the pollution case and cleaning box pass through exhaust tube and connect with aspiration pump, and the pollution case and cleaning box bottom are equipped with
Blowdown valve, the air inlet pipe of the pollution case are equipped with cutting head, and the pollution case is directly connected with the cutting head by air inlet pipe
It connects, collector is equipped between pollution case and the aspiration pump, the air inlet pipe of the cleaning box is equipped with high efficiency particulate air filter；
The cleaning box includes interlude square cavity and upper conical cavity and lower conical cavity；
The pollution case includes interlude square cavity and upper conical cavity and lower conical cavity, the pollution
Case interlude square cavity side is equipped with an air inlet, for supplementing zero air；
The interlude square cavity two sides of the pollution case and cleaning box are uniformly provided with 4 air distribution ports, the pollution case with
Several locking devices are additionally provided on the interlude square cavity of cleaning box；
The cutting head is detachable, replacement selects to be equipped with one of PM2.5, PM10, PM1 according to different requirement of experiment；
Particle sampling film is equipped in the collector；
The high efficiency particulate air filter is stage high-effective filter, is successively arranged level-one vapour from top to bottom in the stage high-effective filter
Automobile-used filter membrane, second level high efficiency filter film, stage high-effective filter membrane；
Less than 1 micron, the stage high-effective filter membrane is HEPA high efficiency filter film, described in the aperture of the second level high efficiency filter film
The aperture of HEPA high efficiency filter film is less than 0.3 micron；
The pollution case and cleaning chamber door are equipped with cylindrical seal item, glass vision panel.
2. animal exposure experimental provision as described in claim 1, which is characterized in that the pollution case and cleaning box material are
Polishing stainless steel.
3. animal exposure experimental provision as described in claim 1, which is characterized in that be equipped on the pollution case and cleaning box
Support frame, support frame as described above bottom end are equipped with 4 idler wheels, and the idler wheel is equipped with brake gear.
4. animal exposure experimental provision as described in claim 1, which is characterized in that also set in the pollution case and cleaning box
There are portable online PM2.5 detection device, humidity sensor, long-range monitoring camera, automatic monitored control system, gas concentration sensing
Device；
The automatic monitored control system includes illumination self-con-tained unit, electronic temperature controller；
The gas concentration sensor can detect PM2.5, PM10, SO2、NO2、O3, CO concentration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811275872.3A CN109303623B (en) | 2018-10-30 | 2018-10-30 | A kind of animal exposure experimental provision |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811275872.3A CN109303623B (en) | 2018-10-30 | 2018-10-30 | A kind of animal exposure experimental provision |
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| Publication Number | Publication Date |
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| CN109303623A CN109303623A (en) | 2019-02-05 |
| CN109303623B true CN109303623B (en) | 2019-08-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811275872.3A Active CN109303623B (en) | 2018-10-30 | 2018-10-30 | A kind of animal exposure experimental provision |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115250936A (en) * | 2022-07-21 | 2022-11-01 | 中国环境科学研究院 | Ozone exposes experimental apparatus |
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| JP2013236596A (en) * | 2012-05-16 | 2013-11-28 | Shibata Biotechnology Inc | Chamber for whole body exposure test and whole body exposure test apparatus using the same |
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| CN203539483U (en) * | 2013-08-13 | 2014-04-16 | 北京慧荣和科技有限公司 | Whole animal body exposure room |
| CN105136619A (en) * | 2015-07-29 | 2015-12-09 | 上海市浦东新区气象局 | Artificial climate environment exposure system and use method thereof |
| KR20160077534A (en) * | 2014-12-23 | 2016-07-04 | 주식회사 에이치시티엠 | Variable Animal Cage for Testing Inhalation Toxicity |
| CN106563311A (en) * | 2016-11-07 | 2017-04-19 | 河南师范大学 | Experimental device for selective high-efficiency removal of pollutants in ambient air and operation method thereof |
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2018
- 2018-10-30 CN CN201811275872.3A patent/CN109303623B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512245A (en) * | 2011-11-24 | 2012-06-27 | 北京大学 | Method for simulating salt aerosol human body exposures and special device thereof |
| JP2013236596A (en) * | 2012-05-16 | 2013-11-28 | Shibata Biotechnology Inc | Chamber for whole body exposure test and whole body exposure test apparatus using the same |
| CN203042539U (en) * | 2012-12-25 | 2013-07-10 | 北京慧荣和科技有限公司 | Animal full-smoke exposing system |
| CN203432981U (en) * | 2013-08-13 | 2014-02-12 | 北京慧荣和科技有限公司 | Multi-concentration animal oral-nasal inhalation dynamic exposure equipment |
| CN203539483U (en) * | 2013-08-13 | 2014-04-16 | 北京慧荣和科技有限公司 | Whole animal body exposure room |
| KR20160077534A (en) * | 2014-12-23 | 2016-07-04 | 주식회사 에이치시티엠 | Variable Animal Cage for Testing Inhalation Toxicity |
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