CN115669835A - Lucid ganoderma and dendrobium officinale anti-fatigue beverage - Google Patents
Lucid ganoderma and dendrobium officinale anti-fatigue beverage Download PDFInfo
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Abstract
The invention provides an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale. The lucid ganoderma and dendrobium officinale anti-fatigue beverage comprises the following components in parts by weight: 1 to 5 parts of lucid ganoderma, 1 to 5 parts of dendrobium officinale, 0.5 to 1.5 parts of taurine, 0.01 to 0.05 part of nicotinic acid, 60.001 to 0.005 part of vitamin B, 1 to 5 parts of sour agent, 20 to 80 parts of sweetening agent and 900 to 1200 parts of water. The lucid ganoderma and dendrobium officinale anti-fatigue beverage prepared by adopting specific raw materials and a specific process is good in stability, has an excellent anti-fatigue effect, and can effectively relieve physical fatigue after sports.
Description
Technical Field
The invention belongs to the technical field of functional beverage processing. More particularly relates to an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale.
Background
The human body often feels fatigue after exercise, physical labor and working time are too long, even some people often feel tired even if the people do not do the activities, and the symptoms are mainly sleepiness, short breath, limb soreness, ache, tiredness, hypodynamia, cramp, slow action, poor coordination, poor accuracy, poor endurance quality and the like, so that the search for anti-fatigue products for daily use or taking is very important.
In addition to the lengthy exercise, physical labor and working time, medical experts believe that there are a variety of causes that cause fatigue in the human body: (1) mental factors: depression or other similar psychiatric disorders are the most common causes of fatigue in humans; (2) pharmaceutical factors: some thiazide diuretics and antidepressants disrupt the balance of the body, leading to a feeling of fatigue; (3) lack of motion: generally, a person has an illusion that fatigue can be avoided by considering that more rest and no movement exist, but the opposite fact is true, the lack of movement causes lack of excitation of the central nervous system of the person, muscle weakness and slow metabolism, and the person is easy to feel fatigue; (4) toxin accumulation in vivo: the physical strength is reduced when the detoxified organs of the body are busy in removing toxins accumulated in the body, and the toxins are caused by partial mercury element infiltration when foods containing pesticides or metal teeth are filled; (5) obesity: too high a body weight may increase the load on the human heart, resulting in a feeling of fatigue; (6) endocrine disorders: can cause the metabolism retardation of the human body and lead people to feel tired; (7) insufficient sleep: in general, a person who sleeps for less than 7 hours or sleeps late for a long time often feels tired.
At present, a lot of anti-fatigue products are available on the market, but most of anti-fatigue products are relatively complex in components, for example, the prior art provides a muscle-strengthening anti-fatigue composition which contains more than ten active ingredients such as kudzuvine root, coffee bean, peptide powder, chinese olive, poria cocos, dried longan pulp, ginseng, mulberry, lucid ganoderma, liquorice, taurine and the like, wherein the coffee bean can cause uncomfortable symptoms such as insomnia, acid regurgitation, heartburn and the like after being taken for a long time, and the composition is complex in components and relatively high in cost, which indicates that the composition is not suitable for daily taking. Therefore, it is necessary to develop an anti-fatigue product which has simple and safe components and low cost and can be taken daily.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale for daily administration.
The above purpose of the invention is realized by the following technical scheme:
the invention provides an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale, which comprises the following components in parts by weight: 1 to 5 parts of lucid ganoderma, 1 to 5 parts of dendrobium officinale, 0.5 to 1.5 parts of taurine, 0.01 to 0.05 part of nicotinic acid, 0.001 to 0.005 part of vitamin B, 1 to 5 parts of sour agent, 20 to 80 parts of sweetening agent and 900 to 1200 parts of water.
Ganoderma (Ganoderma lucidum (Curtis) P.Karst.) is a fungus of Ganoderma of Ganodermataceae. The fruiting body of Ganoderma lucidum is mainly annual, and a few of Ganoderma lucidum is perennial, with stalk and lateral small stalk. Ganoderma is a Chinese traditional famous and precious medicinal material for strengthening body resistance, consolidating constitution, nourishing, strengthening body constitution, invigorating qi, tranquilizing mind, relieving cough and asthma, and can be used for treating giddiness, insomnia, cardiopalmus, short breath, asthenia, cough and asthma, etc. The book Jing cloud: "governing deafness, benefiting joints, keeping spirit, benefiting essence and qi, strengthening bones and muscles, and improving color". According to modern pharmacological research, the lucid ganoderma has the following functions and efficacies: (1) sedation, anticonvulsant: 5-10 g/kg of ganoderma lucidum and spore alcohol-water extracts thereof are respectively injected into abdominal cavities of mice to obviously inhibit the spontaneous activity of the mice and slightly relax muscles; after 30g/kg of ganoderma lucidum and spore alcohol-water extracts thereof are respectively injected into abdominal cavities of mice, the pentobarbital sleep time can be prolonged, the central inhibition effects of reserpine, chlorpromazine and the like can be obviously enhanced, the central excitation effect of the amphetamine is obviously antagonized, and the convulsion of the mice caused by nicotine can be obviously resisted at the same time; (2) enhancing learning and memory ability: 2.5g/kg of ganoderma alcohol-water extract is injected into the abdominal cavity of a mouse, and the Y maze test proves that the ganoderma alcohol-water extract can improve the learning and memory functions of the mouse; (3) enhancing immunity: the few ganoderma lucidum polysaccharides can not only increase the cellular immunity, humoral immunity and nonspecific immunity of normal mice, but also antagonize the low immune function caused by immunosuppressant, antitumor drug, stress and aging, so that the immune function is recovered to normal or nearly normal level, the action mechanism of the few ganoderma lucidum polysaccharides can enhance the activity of lymphocyte DNA polymerase and promote the synthesis of lymphocyte DNA, thereby promoting the proliferation of lymphocyte and increasing the synthesis and secretion of IL-2, and the like, and the research in recent years finds that the ganoderma lucidum polysaccharides can quickly increase the cAMP concentration in macrophages of the mice to trigger the immune function of the mice; (4) promoting the synthesis of nucleic acid and protein: the effects of ganoderma lucidum polysaccharide such as promoting protein synthesis, improving hematopoietic function, inducing cytochrome P450 and the like are beneficial to enhancing the defense function of the organism; the ganoderma lucidum polysaccharide regulates the metabolic function of cells through the influence on the metabolism of nucleic acid and protein, and the function is probably one of the important mechanisms for strengthening the body resistance and consolidating the constitution; (5) delaying senescence: the ganoderma lucidum polysaccharide can obviously prolong the life of fruit flies, rapidly remove free radicals and prolong the capability of division and metabolism of in vitro subculture cells, which suggests that the functions of the ganoderma lucidum polysaccharide such as immunoregulation, promotion of nucleic acid protein anabolism and the like are the action mechanism of the ganoderma lucidum polysaccharide for delaying senescence.
Dendrobium officinale (Dendrobium officinale Kimura et Migo) with upright stem, cylindrical shape, length of 9-35 cm, thickness of 2-4 mm, no branching and multiple sections; the second row of leaves, paper, long round and needle-shaped, the edge and middle rib are often purplish. The dendrobium officinale has the following effects and pharmacological effects: (1) enhancing immunity: traditional Chinese medicine considers that yin and yang balance, qi and blood are smooth, a human body is in a healthy state, and if yin and yang are imbalanced, a plurality of diseases are clustered; the Liang Dynasty pottery Hongjing says that the dendrobium is in 'replenishing essence and supplementing the interior and totally deficient', the Qing Dynasty Xuda Toona emphasizes that the main function of the dendrobium is in 'tonifying kidney yin and calming the nerves, and is a special medicine for treating yin deficiency caused by heat inclusion', pharmacological studies show that the dendrobium officinale has an enhancement effect on non-specific and specific immunity, and can improve the yin deficiency symptom of a human body and improve the immunity of the organism; (2) auxiliary blood sugar reduction: the traditional Chinese medicine refers to a disease with the main symptoms of polydipsia, polyphagia, polyuria, body emaciation after long-term use or sweetness of urine as diabetes, and the diabetes comprises diabetes mellitus; diabetesThe disease is caused by the basic pathogenesis of kidney yin deficiency and lung and stomach dryness heat, the pathological characteristics of qi and yin deficiency, and the treatment method proposed in Huangdi's internal classic is mainly ' nourishing yin, promoting the production of body fluid and balancing yin and yang '; the book Ben Cao Zheng Yun: dendrobe has the effects of annealing, nourishing yin and relieving restlessness, 823060, 8230and relieving thirst and heat and sweat, and is recorded in Chinese medicine dictionary for clearing heat and nourishing yin, is used for treating fever impairment and thirst, and is a special medicine for treating diabetes since ancient times; pharmacological research shows that the dendrobium officinale can reduce the blood sugar of an epinephrine hyperglycemia mouse, increase the content of hepatic glycogen, reduce the blood sugar of a diabetes rat induced by streptozotocin, increase the level of serum insulin and reduce the level of pancreatic hyperglycemia, and shows that the dendrobium officinale has obvious blood sugar reducing effect on the two models; (3) antioxidation: traditional Chinese medicine has many researches on aging, which are pointed out in Huangdi's internal classic: forty years later, and the self-half of yin qi and the decline of life-span, the subsequent students conclude the theories of yin deficiency generating fire and aging, and the theories of body fluid deficiency generating dryness and aging, which all indicate that the theory of nourishing yin and generating body fluid is the essential method for delaying aging; the dendrobium which is recorded in the compendium of materia medica can lighten the body and prolong the life of the dendrobium, namely 82308230, tonify the kidney and benefit the strength and strengthen the muscles and bones, and the dendrobium which is recorded in the compendium of materia medica, namely the dendrobium is proved to have the function of delaying senility by thickly taking the intestines and the stomach, lightening the body and prolonging the life for a long time; the modern medical research provides the theory of 'aging caused by free radicals', the human aging is considered to be closely related to free radical oxidation, and the dendrobium officinale polysaccharide has the inhibition effect on liver microsome lipid peroxidation, has stronger functions of clearing hydroxyl free radicals and superoxide anions and has the effect of H 2 O 2 The induced erythrocyte oxidation hemolysis also has a certain protective effect; the dendrobium officinale shows good antioxidant effect, and the effect of delaying senescence is laterally verified; (4) relieving physical fatigue: the records of the dendrobe for tonifying the five internal organs and consumptive disease and weakness in Shennong's herbal Jing and Ben Cao gang mu, and the records of the dendrobe for tonifying the five internal organs and consumptive disease and weakness in Ri Hua Zi Ben Cao, the medicine nature treatise on nourishing kidney qi and benefiting strength, the Ben Cao gang mu Shi Yi (overstrain) and the Ben Cao Bei Yao (supplement of consumptive disease) indicate that the dendrobe has the effects of resisting fatigue and strengthening the body; modern research shows thatThe dendrobium officinale can obviously prolong the weight bearing swimming time of mice, reduce the levels of blood lactic acid and creatine kinase, improve the exercise endurance and accelerate the elimination of fatigue; (5) improving the gastrointestinal function: the traditional Chinese medicine considers that the spleen and the stomach are the acquired root and the stomach is the sea of the food, and the spleen and the stomach mainly receive, decay and mature the food and the food essence and qi are transformed by the spleen to nourish the whole body; the stomach is yang earth, which is pleased with moistening and disliking dryness; the Shennong Bencao Jing records that the stomach and intestine is taken thickly for a long time, the Liang Dynasty pottery hong Jing Yun Shi is capable of balancing stomach qi and growing muscles, and the Ben Cao Xin considers that the dendrobe special is capable of regulating stomach qi and clearing stomach fire, so the Shennong Ben Cao Jing is called as a gastrointestinal drug; modern pharmacological experiments also prove that the dendrobium candidum crystal prepared by taking the dendrobium candidum as the main raw material can obviously promote gastric secretion of rats, increase gastric acid and pepsin output, enhance small intestine propulsion of mice and soften excrement.
The invention carries out a great deal of research on the anti-fatigue efficacy and the components of the ganoderma lucidum and the dendrobium officinale, prepares a proper proportion and an extraction process, and perfectly compounds the components such as taurine, nicotinic acid and the like to design the formula of the anti-fatigue drink of the ganoderma lucidum and the dendrobium officinale.
Preferably, the formula of the anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale comprises the following components in parts by weight: 1 to 3 parts of lucid ganoderma, 1 to 3 parts of dendrobium officinale, 0.5 to 1.5 parts of taurine, 0.03 to 0.05 part of nicotinic acid, 0.003 to 0.005 part of vitamin B, 1 to 2 parts of sour agent, 35 to 80 parts of sweetening agent and 950 to 1100 parts of water.
Most preferably, the formula of the anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale comprises the following components in parts by weight: 3 parts of lucid ganoderma, 3 parts of dendrobium officinale, 1 part of taurine, 0.04 part of nicotinic acid, 0.004 part of vitamin B, 1.9 parts of sour agent, 65 parts of sweetening agent and 1000 parts of water.
Preferably, the acidulant includes, but is not limited to, citric acid and the like; such sweeteners include, but are not limited to, white granulated sugar and the like.
Preferably, the preparation method of the lucid ganoderma and dendrobium officinale anti-fatigue beverage comprises the following steps:
s1, uniformly mixing the ganoderma lucidum and the dendrobium officinale in a formula amount, adding water, heating at 55-65 ℃ for 20-30 min, heating to 75-80 ℃ at the speed of 2-5 ℃/min, keeping for 8-12 min, cooling to 60-65 ℃ at the speed of 1-3 ℃/min, keeping for 10-20 min, and separating to obtain a decoction;
s2, dissolving taurine, nicotinic acid, vitamin B6, a sour agent and a sweetening agent in the boiling liquid obtained in the step S1, adding water, and sterilizing to obtain the lucid ganoderma-dendrobium officinale anti-fatigue beverage.
Preferably, before the water is added into the S1, the lucid ganoderma and the dendrobium officinale are filled into a filter bag.
Further preferably, the separation is filtration or removal of a filter bag.
Further preferably, the filter bag is a sealable cotton bag.
Preferably, S1 is: mixing Ganoderma and herba Dendrobii uniformly, adding water, heating at 60 deg.C for 25min, heating to 75 deg.C at 3 deg.C/min, maintaining for 10min, cooling to 60 deg.C at 3 deg.C/min, maintaining for 15min, and separating to obtain decoction.
Preferably, S2 is: firstly, dissolving a sweetening agent in water at 60-65 ℃ to obtain a solution A; dissolving taurine, nicotinic acid, vitamin B6 and an acidulant in other water with the temperature of 60-65 ℃ to obtain a solution B; and finally, adding the solution A and the solution B into the cooking liquor obtained in the step S1, adding water, and sterilizing to obtain the anti-fatigue beverage containing the ganoderma lucidum and the dendrobium officinale.
Further preferably, S2 is: firstly, dissolving a sweetening agent in water with the temperature of 60 ℃ to obtain a solution A; dissolving taurine, nicotinic acid, vitamin B6 and sour agent in water with the temperature of 60 ℃ to obtain solution B; and finally, adding the solution A and the solution B into the cooking liquor obtained in the step S1, adding water, and sterilizing to obtain the anti-fatigue beverage containing the ganoderma lucidum and the dendrobium officinale.
Preferably, the sterilization of S2 is performed at 120-150 ℃ for 10-30S, and most preferably at 130 ℃ for 15S.
The invention has the following beneficial effects:
1. the lucid ganoderma and dendrobium officinale anti-fatigue beverage prepared by adopting specific raw materials and a specific process is good in stability, has an excellent anti-fatigue effect, and can effectively relieve physical fatigue after sports.
2. The invention adopts a specific multi-section temperature-changing process, realizes the simultaneous extraction of the ganoderma lucidum and the dendrobium officinale, effectively relieves the bitter taste of the traditional Chinese medicine, and improves the taste of the beverage.
3. The anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale provided by the invention is simple and safe in ingredients, scientific in formula, pure in flavor, natural and harmless, simple in production process and low in cost, and can be taken daily.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Embodiment 1 an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale
1. The components are as follows:
the lucid ganoderma and dendrobium officinale anti-fatigue beverage (500 mL) comprises the following components in parts by weight: 3 parts of lucid ganoderma, 3 parts of dendrobium officinale, 1 part of taurine, 0.04 part of nicotinic acid, 0.004 part of vitamin B, 1.9 parts of sour agent, 65 parts of sweetening agent and 1000 parts of water.
2. The preparation method comprises the following steps:
s1, uniformly mixing the ganoderma lucidum and the dendrobium officinale according to the formula amount, wrapping the mixture by a cotton cloth bag which can be sealed and is 50cm multiplied by 50cm and is 200 meshes, putting the mixture into a cooking pot, adding water, heating the mixture at 60 ℃ for 25min, heating the mixture to 75 ℃ at the speed of 3 ℃/min, keeping the temperature for 10min, cooling the mixture to 60 ℃ at the speed of 3 ℃/min, keeping the temperature for 15min, transferring a cotton cloth bag wrapped with the traditional Chinese medicines to the position right above the cooking pot, and draining the water to obtain cooking liquid.
S2, dissolving white granulated sugar in water at 60 ℃ to obtain a solution A; dissolving taurine, nicotinic acid, vitamin B6 and citric acid in water at the temperature of 60 ℃ to obtain a solution B; and finally adding the solution A and the solution B into the cooking liquor obtained in the step S1, uniformly mixing, adding water to a formula amount, filling into a PET bottle, and sterilizing by adopting a high-temperature instantaneous sterilization method (sterilizing for 15S at 130 ℃) to obtain the lucid ganoderma and dendrobium officinale anti-fatigue beverage.
Embodiment 2 an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale
1. The components are as follows:
the lucid ganoderma and dendrobium officinale anti-fatigue beverage (500 mL) comprises the following components in parts by weight: 1 part of lucid ganoderma, 3 parts of dendrobium officinale, 1.5 parts of taurine, 0.03 part of nicotinic acid, 0.003 part of vitamin B, 2 parts of citric acid, 80 parts of white granulated sugar and 1100 parts of water.
2. The preparation method comprises the following steps:
the preparation method is the same as example 1.
Embodiment 3 anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale
1. The components are as follows:
the lucid ganoderma and dendrobium officinale anti-fatigue beverage (500 mL) comprises the following components in parts by weight: 3 parts of lucid ganoderma, 1 part of dendrobium officinale, 0.5 part of taurine, 0.05 part of nicotinic acid, 0.005 part of vitamin B, 1 part of citric acid, 35 parts of white granulated sugar and 950 parts of water.
2. The preparation method comprises the following steps:
the preparation method is the same as example 1.
Embodiment 4 an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale
1. The components are as follows:
the lucid ganoderma and dendrobium officinale anti-fatigue beverage (500 mL) comprises the following components in parts by weight: 5 parts of lucid ganoderma, 1 part of dendrobium officinale, 1.5 parts of taurine, 0.01 part of nicotinic acid, 0.005 part of vitamin B, 1 part of citric acid, 20 parts of white granulated sugar and 900 parts of water.
2. The preparation method comprises the following steps:
s1, uniformly mixing the ganoderma lucidum and the dendrobium officinale in a formula amount, wrapping the mixture by a cotton cloth bag which can be sealed and is 50cm multiplied by 50cm and is 200 meshes, putting the mixture into a cooking pot, adding water, heating the mixture at 55 ℃ for 30min, heating the mixture to 80 ℃ at the speed of 5 ℃/min, keeping the temperature for 8min, cooling the mixture to 65 ℃ at the speed of 1 ℃/min, keeping the temperature for 10min, moving a cotton bag wrapped with traditional Chinese medicines to the position right above the cooking pot, and draining the water to obtain cooking liquor;
s2, dissolving white granulated sugar in water at 60 ℃ to obtain a solution A; dissolving taurine, nicotinic acid, vitamin B6 and citric acid in water at the temperature of 60 ℃ to obtain a solution B; and finally, adding the solution A and the solution B into the boiling liquid obtained in the step S1, uniformly mixing, adding water to the formula amount, filling the mixture in a PET bottle, and sterilizing by adopting a high-temperature instantaneous sterilization method (sterilizing at 120 ℃ for 30S) to obtain the lucid ganoderma-dendrobium officinale anti-fatigue beverage.
Embodiment 5 an anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale
1. The components are as follows:
the lucid ganoderma and dendrobium officinale anti-fatigue beverage (500 mL) comprises the following components in parts by weight: 1 part of lucid ganoderma, 5 parts of dendrobium officinale, 0.5 part of taurine, 0.05 part of nicotinic acid, 0.001 part of vitamin B, 5 parts of citric acid, 80 parts of white granulated sugar and 1200 parts of water.
2. The preparation method comprises the following steps:
s1, uniformly mixing the ganoderma lucidum and the dendrobium officinale in a formula amount, wrapping the mixture by a cotton cloth bag which can be sealed and is 50cm multiplied by 50cm and is 200 meshes, putting the mixture into a cooking pot, adding water, heating the mixture at 65 ℃ for 20min, heating the mixture to 75 ℃ at the speed of 2 ℃/min, keeping the temperature for 12min, cooling the mixture to 60 ℃ at the speed of 3 ℃/min, keeping the temperature for 20min, moving a cotton bag wrapped with traditional Chinese medicines to the position right above the cooking pot, and draining the water to obtain cooking liquid;
s2, dissolving white granulated sugar in 65 ℃ water to obtain a solution A; dissolving taurine, nicotinic acid, vitamin B6 and citric acid in water with the temperature of 65 ℃ to obtain solution B; and finally, adding the solution A and the solution B into the boiling liquid obtained in the step S1, uniformly mixing, adding water to the formula amount, filling the mixture in a PET bottle, and sterilizing by adopting a high-temperature instantaneous sterilization method (sterilizing at 150 ℃ for 10S) to obtain the lucid ganoderma-dendrobium officinale anti-fatigue beverage.
Comparative example 1
The same as example 1, except that ganoderma lucidum was replaced with american ginseng.
Comparative example 2
The difference from example 1 is that the cotton bag in S1 is replaced with a nylon bag.
Comparative example 3
The same as example 1 except that no Ganoderma lucidum is added, and the kinds and amounts of the other components are the same as example 1.
Comparative example 4
The same as example 1, except that dendrobium officinale is not added, and the types and the use amounts of the other components are the same as those of example 1.
Comparative example 5
The difference from the example 1 is that the ganoderma lucidum and the dendrobium officinale are not added, the types and the dosage of the other components are the same as the example 1,
comparative example 6
The difference from example 1 is that instead of changing the temperature in S1 in multiple stages, heating is performed at 75 ℃ for 60min, i.e. S1 is: mixing Ganoderma and herba Dendrobii uniformly, wrapping with a cotton bag of 50cm × 50cm (mesh size) of 200 meshes, putting into a material cooking pot, adding water, heating at 75 deg.C for 60min, transferring the cotton bag wrapped with Chinese medicinal materials to the position right above the material cooking pot, and draining to obtain the decoction.
Comparative example 7
The difference from example 1 is that instead of heating at 90 ℃ for 60min the multi-stage temperature change in S1, i.e. S1 is: mixing Ganoderma and herba Dendrobii uniformly, wrapping with a cotton cloth bag of 50cm × 50cm with 200 mesh, placing into a material cooking pot, adding water, heating at 90 deg.C for 60min, transferring the cotton cloth bag wrapped with traditional Chinese medicines to the right top of the material cooking pot, and draining to obtain the decoction.
Experimental example 1 stability test
Two portions of the beverages obtained in examples 1 to 5 and comparative examples 1 to 7 were stored at 4 ℃ and 25 ℃ for 12 months, respectively, and the stability of the beverages was observed at 1 month, 4 months, 7 months, 11 months, and 12 months, and the results are shown in Table 1.
TABLE 1 stability of beverages
As can be seen from Table 1, the stability of the beverage can be still maintained when the beverage is placed at 4 ℃ and 25 ℃ for 12 months in examples 1 to 5 and comparative examples 1 and 3 to 7, while a small amount of floccules appear in comparative example 2 in which the traditional Chinese medicine is wrapped by the nylon bag under the storage condition of 25 ℃ for 4 months, and the floccules are generated in 7 months even if the beverage is stored in a refrigerated manner, which indicates that the stability of the beverage can be effectively ensured only when the cotton bag is specifically selected for the anti-fatigue beverage of the ganoderma dendrobium officinale.
Experimental example 2 sensory evaluation of color, sweetness, acidity, bitterness and astringency, and mouth feel
The beverages of examples 1 to 5 and comparative examples 1 to 7 were evaluated by an evaluation group consisting of 20 assessors in the interior of Dongpo, in terms of color, sweetness, acidity, bitterness and astringency, and taste, and scored according to the scoring criteria of Table 2, and the results are shown in Table 3 after averaging the scores.
TABLE 2 color, sweetness, acidity, bitterness and taste evaluation criteria (unit: points)
TABLE 3 color, sweetness, acidity, bitterness, and mouthfeel scoring results (unit: point)
As can be seen from Table 3, the beverages of examples 1-5 have pure and uniform color, moderate sweetness and sourness, slight bitterness and astringency and good mouthfeel, the total score reaches 83.9-91.5, and particularly the beverage of example 1 has the best score, which is obviously superior to that of comparative examples 1-7. The preparation methods of the comparative examples 6 and 7 do not adopt multi-stage temperature variation, but the two different traditional Chinese medicines of the lucid ganoderma and the dendrobium officinale are heated in a one-stage manner at the same temperature, so that the bitter taste of the traditional Chinese medicines cannot be relieved, the bitter taste is heavier, and the taste is not good.
Experimental example 3 anti-fatigue efficacy
1. Sensory evaluation
Screening of test subjects: recruiting 120 adult students (18-22 years old, playing basketball for three days every week and 0.5-1 hour every day) for playing basketball for a long time from a school;
the test subjects were randomly divided into 12 groups, and after continuously basketball for 1 hour every day for 3 consecutive days, the beverages of examples 1 to 5 and comparative examples 1 to 7 were respectively drunk, and the anti-fatigue effect of the beverages was scored according to the anti-fatigue effect scoring criteria shown in table 4, and the results after averaging the scores were shown in table 5.
TABLE 4 score Standard for antifatigue Effect (Unit: point)
| Standard of merit | Score of |
| Has very good anti-fatigue effect | 9～10 |
| Has better anti-fatigue effect | 7～8 |
| The anti-fatigue effect is general | 5～6 |
| The anti-fatigue effect is weak or not | 1～4 |
TABLE 5 score results for anti-fatigue Effect (unit: point)
As can be seen from Table 5, the anti-fatigue effects of the examples 1-5 are very good and are remarkably superior to those of the comparative examples 1-7, wherein the anti-fatigue effects of the comparative example 3 lacking the lucid ganoderma, the comparative example 4 lacking the dendrobium officinale and the comparative example 5 lacking the lucid ganoderma and the dendrobium officinale are remarkably inferior to those of the example 1, and the results show that the lucid ganoderma and the dendrobium officinale of the example 1 synergistically exert excellent anti-fatigue effects.
2. Animal experiments
(1) Experimental materials:
130 clean-grade Kunming mice, male, 18 g-22g, free diet and drinking water at 25 ℃, and random average division into 13 groups.
Referring to the beverage formulas of examples 1 to 5 and comparative examples 1 to 7, respectively, but after white granulated sugar and citric acid are removed, the rest are the same, and beverage products are prepared, which correspond to beverage 1 of the experimental group to beverage 5 of the experimental group and beverage 1 of the control group to beverage 7 of the control group respectively.
(2) Grouping:
blank group, experiment group 1-experiment group 5, and control group 1-control group 7.
(3) The experimental method comprises the following steps:
after the experimental group beverage 1 to the experimental group beverage 5 and the control group beverage 1 to the control group beverage 7 were concentrated (dehydrated) by 12.5 times respectively:
experimental group 1: the experimental group drink 1 is infused with stomach 1 time a day, the stomach infusion amount is 6.67mL/kg each time,
experimental group 2: the beverage 2 of the experimental group is infused 1 time a day, the infusion amount is 6.67mL/kg each time,
experimental group 3: the experimental group drink 3 is infused with stomach 1 time a day, the stomach infusion amount is 6.67mL/kg each time,
experimental group 4: the experimental group drink 4 is infused with stomach 1 time a day, the stomach infusion amount is 6.67mL/kg each time,
experimental group 5: the experimental group drink 5 is infused in stomach 1 time a day, the stomach infusion amount is 6.67mL/kg each time,
control group 1: the contrast group beverage 1 is infused 1 time per day, the volume of the infused stomach is 6.67mL/kg each time,
control group 2: the contrast group beverage 2 is infused 1 time per day, the volume of the infused stomach is 6.67mL/kg each time,
control group 3: the contrast group beverage 3 is infused in stomach 1 time a day, the stomach infusion amount is 6.67mL/kg each time,
control group 4: the control group beverage is infused 1 time a day with the infusion amount of 6.67mL/kg each time,
control group 5: the control group beverage 5 is infused 1 time per day, the volume of the infused stomach is 6.67mL/kg each time,
control group 6: the contrast group beverage 6 is infused 1 time per day, the volume of the infused stomach is 6.67mL/kg each time,
control group 7: the control group beverage 7 is infused 1 time per day, the volume of the infused stomach is 6.67mL/kg each time,
blank group: no treatment (no gavage) was performed.
The results of the weight swimming test and the measurement of blood lactic acid, serum urea nitrogen and liver glycogen after 30 consecutive days of the above treatment (while eating freely and drinking water) are shown in Table 6.
TABLE 6 results of animal experiments
| Swimming time (min) | Blood lactic acid (mol/L) | Serum urea nitrogen (mg/L) | Hepatic glycogen (mg/g) | |
| Blank group | 10.3±4.3 | 25.4±1.4 | 460.5±20.5 | 12.5±3.1 |
| Experimental group 1 | 34.5±5.8 | 18.5±3.6 | 368.2±23.6 | 18.9±0.2 |
| Experimental group 2 | 33.4±1.3 | 19.5±0.3 | 381.3±31.7 | 17.3±5.5 |
| Experimental group 3 | 34.1±5.9 | 19.3±3.7 | 395.4±51.4 | 16.7±8.9 |
| Experimental group 4 | 33.6±4.8 | 20.3±2.2 | 403.2±12.8 | 16.3±4.6 |
| Experimental group 5 | 32.6±8.5 | 20.9±5.1 | 411.9±21.6 | 16.2±6.1 |
| Control group 1 | 27.1±9.1 | 22.9±6.0 | 429.5±60.9 | 14.2±9.7 |
| Control group 2 | 30.4±7.5 | 21.1±0.4 | 416.4±33.8 | 15.7±6.8 |
| Control group 3 | 22.6±4.1 | 23.9±2.0 | 437.6±48.7 | 13.2±7.6 |
| Control group 4 | 20.1±5.6 | 24.2±1.5 | 441.8±15.4 | 13.0±5.4 |
| Control group 5 | 16.5±6.7 | 24.6±7.9 | 443.5±73.5 | 12.7±0.8 |
| Control group 6 | 29.4±2.1 | 21.8±2.5 | 420.1±20.4 | 15.3±5.2 |
| Control group 7 | 28.3±6.4 | 22.0±5.3 | 418.6±58.5 | 14.9±4.5 |
As can be seen from Table 6, the mice of the experimental groups 1-5 are remarkably superior to the mice of the control groups 1-7 in the aspects of the weight-bearing swimming time, the blood lactic acid content, the serum urea nitrogen content and the liver glycogen content, and therefore, the anti-fatigue drink containing the ganoderma dendrobium officinale and the dendrobium officinale prepared by adopting the specific raw materials and the specific process has excellent antibody fatigue efficacy.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. The lucid ganoderma and dendrobium officinale anti-fatigue beverage is characterized by comprising the following components in parts by weight: 1 to 5 parts of glossy ganoderma, 1 to 5 parts of dendrobium officinale, 0.5 to 1.5 parts of taurine, 0.01 to 0.05 part of nicotinic acid, 0.001 to 0.005 part of vitamin B, 1 to 5 parts of sour additive, 20 to 80 parts of sweetening agent and 900 to 1200 parts of water.
2. The lucid ganoderma-dendrobium candidum anti-fatigue beverage as claimed in claim 1, which is characterized by comprising the following components in parts by weight: 1 to 3 parts of lucid ganoderma, 1 to 3 parts of dendrobium officinale, 0.5 to 1.5 parts of taurine, 0.03 to 0.05 part of nicotinic acid, 0.003 to 0.005 part of vitamin B, 1 to 2 parts of sour agent, 35 to 80 parts of sweetening agent and 950 to 1100 parts of water.
3. The anti-fatigue beverage with lucid ganoderma and dendrobium officinale as claimed in claim 1, wherein the sour agent comprises citric acid; the sweetener comprises white granulated sugar.
4. The anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale as claimed in claim 1, wherein the preparation method comprises the following steps:
s1, uniformly mixing the lucid ganoderma and the dendrobium officinale in a formula amount, adding water, heating at 55-65 ℃ for 20-30 min, heating to 75-80 ℃ at the speed of 2-5 ℃/min, keeping for 8-12 min, cooling to 60-65 ℃ at the speed of 1-3 ℃/min, keeping for 10-20 min, and separating to obtain a decoction;
s2, dissolving taurine, nicotinic acid, vitamin B6, a sour agent and a sweetening agent in the boiled liquid obtained in the step S1, adding water, and sterilizing to obtain the lucid ganoderma and dendrobium officinale anti-fatigue beverage.
5. The anti-fatigue lucid ganoderma and dendrobium officinale beverage as claimed in claim 4, wherein the lucid ganoderma and the dendrobium officinale are packaged in a filter bag before water is added to the beverage S1.
6. The anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale as claimed in claim 5, wherein the separation is filtration or a filter bag is taken out.
7. The anti-fatigue lucid ganoderma and dendrobium officinale beverage as claimed in claim 5, wherein the filter bag is a sealable cotton bag.
8. The anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale as claimed in claim 4, wherein S1 is: mixing Ganoderma and herba Dendrobii uniformly, adding water, heating at 60 deg.C for 25min, heating to 75 deg.C at 3 deg.C/min, maintaining for 10min, cooling to 60 deg.C at 3 deg.C/min, maintaining for 15min, and separating to obtain decoction.
9. The anti-fatigue lucid ganoderma and dendrobium officinale beverage as claimed in claim 4, wherein S2 is as follows: firstly, dissolving a sweetening agent in water with the temperature of 60-65 ℃ to obtain a solution A; dissolving taurine, nicotinic acid, vitamin B6 and an acidulant in other water with the temperature of 60-65 ℃ to obtain a solution B; and finally, adding the solution A and the solution B into the cooking liquor obtained in the step S1, adding water, and sterilizing to obtain the anti-fatigue beverage containing the ganoderma lucidum and the dendrobium officinale.
10. The anti-fatigue beverage containing ganoderma lucidum and dendrobium officinale as claimed in claim 4, wherein the sterilization of S2 is performed at 120-150 ℃ for 10-30S.
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| CN104509766A (en) * | 2014-12-23 | 2015-04-15 | 金华寿仙谷药业有限公司 | Dendrobium officinale beverage and preparation method thereof |
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| CN113229431A (en) * | 2021-05-28 | 2021-08-10 | 西藏大学 | White-meat lucid ganoderma sports beverage and preparation method thereof |
| CN113975353A (en) * | 2021-09-07 | 2022-01-28 | 河南高科智慧健康药食研究院有限公司 | Medicine-food homologous nutrient solution formula for reducing uric acid and treating gout and preparation process thereof |
| CN114403321A (en) * | 2021-12-23 | 2022-04-29 | 百色学院 | Preparation method of dendrobium officinale and ganoderma lucidum compound health-care beverage |
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| CN104509766A (en) * | 2014-12-23 | 2015-04-15 | 金华寿仙谷药业有限公司 | Dendrobium officinale beverage and preparation method thereof |
| CN107982439A (en) * | 2017-12-22 | 2018-05-04 | 营养屋(成都)生物医药有限公司 | The extracting method of dendrobium candidum American ginseng capsule, its preparation method and dendrobium candidum |
| CN113229431A (en) * | 2021-05-28 | 2021-08-10 | 西藏大学 | White-meat lucid ganoderma sports beverage and preparation method thereof |
| CN113975353A (en) * | 2021-09-07 | 2022-01-28 | 河南高科智慧健康药食研究院有限公司 | Medicine-food homologous nutrient solution formula for reducing uric acid and treating gout and preparation process thereof |
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