CN115644435A - Qi-tonifying and beautifying composition and preparation method and application thereof - Google Patents

Abstract

The invention provides a qi-tonifying and beauty-maintaining composition which comprises, by mass, 10-15% of acerola cherry, 1-5% of cubilose peptide, 5-10% of medlar, 1-10% of Chinese date, 10-20% of ginseng, 1-5% of donkey-hide gelatin, 5-10% of collagen, 1-5% of royal jelly and 1-3% of rose with double petals. According to the method, the acerola cherry powder is treated in a first steam blanching mode, so that the vitamin C retention rate in the manufacturing process is effectively improved; the application finds that the papain is helpful for degrading macromolecular components in acerola and helps vitamin C to be absorbed and utilized by human bodies; through the combination of microwave radiation and spray drying, the molecular weight of the cubilose peptide is reduced, so that the cubilose peptide is better absorbed and utilized by a human body, and the problem that the acerola cherry is loose in structure and not easy to tablet and form after being mixed with other components is also solved.

Description

Qi-tonifying and beautifying composition and preparation method and application thereof

Technical Field

The application relates to the technical field of health products, in particular to a qi-tonifying and beauty-maintaining composition and a preparation method and application thereof.

Background

The edible bird's nest mainly comprises water, ash, fat, protein and saccharide, and comprises five kinds of semi-essential amino acids and six kinds of essential amino acids. After the protein in the cubilose is soaked, boiled, and soaked, a large amount of insoluble protein exists, and the protein is difficult to absorb by a human body. In order to solve the problem, some cubilose polypeptide beverages are also provided in the market at present, but the cubilose polypeptide beverage decomposes cubilose into cubilose polypeptides, but still has the problems of poor absorption and short shelf life, and the problems of sticking, layering and the like also exist when the cubilose polypeptides are pressed into tablets.

Vitamin C (vitamin C) is abundant in fruits and vegetables, and its deficiency can cause scurvy, and is called anti-scurvy vitamin. With the wide application of vitamin C, people find that the vitamin C also has the effects of resisting oxidation, resisting free radicals and inhibiting the formation of tyrosinase, and has the effects of whitening and fading spots. However, in the practical application process, the vitamin C is found to have unstable properties and be easily damaged by oxidation.

Chinese patent application CN 109619509A discloses a rose collagen jelly and a preparation method thereof, the jelly product solves the original fishy smell of the collagen jelly, and the effects of maintaining beauty and keeping young of the product are enhanced by combining collagen with traditional Chinese medicines for promoting qi circulation, tonifying qi, nourishing yin and blood, but the jelly product has higher storage requirement and limited product storage time.

Disclosure of Invention

The invention aims to provide a composition for tonifying qi and beautifying, which combines the components of medicines, foods, collagen and the like for tonifying qi and beautifying to realize synergistic effect, and is used for tonifying qi and beautifying.

On one hand, the composition for tonifying qi and beautifying comprises, by mass, 10-15% of acerola cherry, 1-5% of cubilose peptide, 5-10% of medlar, 1-10% of Chinese date, 10-20% of ginseng, 1-5% of donkey-hide gelatin, 5-10% of collagen, 1-5% of royal jelly and 1-3% of rose with multiple petals.

Further, the composition comprises, by mass, 10.7% of acerola cherry, 1.33% of cubilose peptide, 6.67% of medlar, 5.33% of Chinese date, 13.3% of ginseng, 4% of donkey-hide gelatin, 9.33% of collagen, 1.33% of royal jelly and 1.33% of rose with double petals.

Further, the composition for tonifying qi and beautifying also comprises a sweetening agent, wherein the sweetening agent is one or more of sorbitol, D-mannitol, erythritol and lactitol; preferably, sorbitol accounting for 25 to 30% of the total mass, 15 to 20% of D-mannitol; more preferably, 28% sorbitol, 17.7% D-mannitol.

Furthermore, the qi-tonifying and face-beautifying composition also comprises an auxiliary agent, wherein the auxiliary agent is 1% of magnesium stearate.

In a preferred embodiment, a qi-tonifying and skin-caring composition comprises 10.7% acerola cherry, 1.33% bird's nest peptide, 6.67% medlar, 5.33% jujube, 13.3% ginseng, 4% donkey-hide gelatin, 9.33% collagen, 1.33% royal jelly, 1.33% rose, 28% sorbitol, 17.7% d-mannitol, 1% magnesium stearate.

In a preferred embodiment, the tablet for tonifying qi and beautifying comprises the following specific single-particle dosage formula: 50mg of medlar, 40mg of Chinese date, 100mg of ginseng, 30mg of donkey-hide gelatin, 70mg of collagen, 10mg of cubilose peptide, 10mg of royal jelly freeze-dried powder, 80mg of acerola powder, 10mg of rose powder with multiple petals, 210mg of sorbitol, 132.5mg of D-mannitol and 7.5mg of magnesium stearate.

On the other hand, the application also provides a preparation method of the qi-tonifying and beauty-maintaining composition, which comprises the following steps:

step one, grinding acerola cherry to 40-60 meshes, spraying an enzyme preparation, reacting at 37-40 ℃ for 4-6 h, steam blanching at 80-95 ℃ for 4-6 min, and mixing with medlar, chinese date, ginseng and rose to obtain first powder; preferably, steam blanching for 5min;

step two, dissolving the bird's nest peptide in water, uniformly mixing, performing microwave radiation, and performing vacuum low-temperature spray drying to obtain second powder;

and step three, mixing the first powder and the second powder, adding donkey-hide gelatin, collagen, royal jelly, sorbitol, D-mannitol and magnesium stearate, and uniformly mixing to obtain the composition.

Further, the enzyme preparation is aqueous solution of papain with the concentration of 10-15%, preferably, 10%;

the addition amount of the enzyme preparation is 10 percent of the dry matter of the acerola cherry in percentage by mass.

According to the application, the acerola powder is treated by the papain for the first time, and the papain is found to be helpful for degrading macromolecular components in the acerola, so that the absorbable utilization content of the vitamin C is improved, and the vitamin C is better absorbed and utilized by a human body. And subsequently, the papain is inactivated in a steam blanching way, so that other active ingredients are prevented from being damaged by excessive reaction of an enzyme preparation, the effective content of the vitamin C is protected to the maximum extent, and the storage time of the vitamin C in the tablet can be effectively prolonged.

Further, the mass ratio of the cubilose peptide to the water is 1: (4-5); preferably, 1:5.

Further, the spray drying parameters are: the inlet air temperature is 100-120 ℃, the outlet temperature is 80-90 ℃, the inlet air flow is 4-5 m ³ Min; preferably, the inlet air temperature is 120 ℃, the outlet temperature is 90 ℃, the inlet air flow is 5m ³ /min。

According to the application, the molecular weight of the cubilose peptide is reduced through the combination of microwave radiation and spray drying, so that the cubilose peptide is better absorbed and utilized by a human body, and the effects of beautifying, resisting aging and whitening of the composition are enhanced. The composition of the processed cubilose peptide and other components has high tablet yield and is suitable for application and production.

In a preferred embodiment, a method for preparing a qi-tonifying and beauty-maintaining composition comprises the following steps:

crushing acerola cherry dry matter, sieving with a 40-60-mesh sieve, spraying aqueous solution of papain, spraying the aqueous solution of papain with the spraying amount of 10 percent of the total mass of the powder, performing enzymolysis at 37-40 ℃ for 4-6 h, performing steam blanching for 5min (90 +/-0.5 ℃), and mixing with Chinese wolfberry dry matter, chinese date dry matter, ginseng dry matter and rose dry matter;

step two, taking the cubilose peptide and water, uniformly mixing the cubilose peptide and the water according to the mass ratio of 1:5, performing vacuum low-temperature spray drying after microwave radiation, wherein the spray drying parameters are as follows: the inlet air temperature is 120 ℃, the outlet temperature is 90 ℃, the inlet air flow is 5m ³ /min；

And step three, mixing the powder obtained in the step one and the powder obtained in the step two, adding donkey-hide gelatin powder, collagen powder, royal jelly freeze-dried powder, sorbitol, D-mannitol and magnesium stearate, uniformly mixing, and tabletting, wherein the weight of the tablet is 0.8g.

Wherein the enzyme preparation is 10% papain aqueous solution.

Microwave radiation: the frequency can be controlled between 300 MHz and 500MHz

In another aspect, the present application also provides a health food comprising the above composition;

preferably, the dosage form of the health food is tablets, capsules or granules; more preferably, a tablet.

It will be appreciated that other known ingredients, such as plasticizers, taste masking agents, etc., may be added to the tablet formulation of the present application by those skilled in the art.

On the other hand, the application also provides application of the health food in tonifying qi and beautifying.

Especially in anti-aging, whitening, qi-replenishing and blood-enriching.

The invention has the following beneficial effects:

1. according to the method, the acerola cherry powder is treated by adopting a first steam blanching mode, so that the vitamin C retention rate in the preparation process is effectively improved, and a new way is provided for producing vitamin C products with high vitamin content and long quality guarantee period;

2. according to the application, the papain is beneficial to degrading macromolecular components in acerola, the vitamin C content in the product can be improved, the papain is inactivated by matching with a steam blanching treatment mode, other effective components can be prevented from being damaged by excessive reaction of an enzyme preparation, and the vitamin C content in the final product is improved;

3. through microwave radiation and spray drying ally oneself with usefulness in this application, with the reduction of bird's nest peptide molecular weight, make it better by human absorption utilization, make things convenient for the preforming more, easily production preparation.

Detailed Description

The following detailed description is given by way of example in order to more clearly illustrate the general concept of the present application. In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.

The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer.

Wherein, the acerola cherry dry matter, the medlar powder, the Chinese date powder, the ginseng powder and the rose powder with double petals are provided by An Xu Huang biotechnology limited company; colla Corii Asini powder is provided by Shandong colla Corii Asini products, inc.; the collagen powder is bovine bone collagen and is provided by Woltelis biotechnology, inc. of Lanzhou; nidus Collocaliae peptide is provided by Shenzhen Lefu Biotech, inc.; the lyophilized royal jelly powder is provided by Mufang Biotechnology GmbH of Xinyang; sorbitol, D-mannitol, and magnesium stearate were provided by Xian jin source Biotech Co., ltd.

In the following embodiments, reagents or instruments used are not indicated by manufacturers, and are all conventional products available by commercial purchase, unless otherwise specified.

EXAMPLE 1 vitamin C assay

And (3) crushing dry acerola cherry substances, sieving the crushed dry acerola cherry substances by a sieve of 40-60 meshes, and blanching the crushed dry acerola cherry substances by steam. Acerola dry matter without steam blanching was used as a blank control (treatment time 0 min). And respectively measuring the vitamin C content in the blank control group and the treated acerola powder and the vitamin C content in the acerola powder after being placed for 15 days at 25 ℃. The specific determination method comprises the following steps: according to GB 5413.18 determination of vitamin C in infant food and dairy products, which is the national standard for food safety, vitamin C is oxidized into dehydroascorbic acid in the presence of activated carbon, and reacts with o-phenylenediamine to generate a fluorescent substance, the fluorescence intensity of the fluorescent substance is measured by a fluorescence spectrophotometer, and is in direct proportion to the concentration of the vitamin C, and the measurement result is quantitatively determined by an external standard method and is shown in Table 1.

TABLE 1

As can be seen from the results in Table 1, the preservation time of vitamin C in the tablets can be effectively prolonged by treating the acerola powder in a steam blanching way.

Example 2 enzyme treatment experiment

Also, it has been found herein that under the preferred conditions of example 1, treating acerola cherry flour with an enzyme prior to blanching helps to increase the water-soluble vitamin C content of the product. See table 2 for details.

The specific method comprises the following steps: taking acerola cherry dry matter, crushing, sieving by a 40-60 mesh sieve, spraying an aqueous solution of an enzyme preparation, wherein the mass ratio of the enzyme to the acerola cherry dry matter is 1:100 Enzymolysis is carried out for 4 to 6 hours at the temperature of between 37 and 40 ℃.

TABLE 2

Enzyme	Vitamin C content/mg kg -1
		Papain	260
Neutral protease	243
		Trypsin	247
Alkaline protease	255
		-	238

As can be seen from the results in Table 2, the selection of papain to treat acerola cherry flour helps to increase the water-soluble vitamin C content of the product.

Test example 1

The tablet for tonifying qi and beautifying comprises the following specific single-particle dosage formula: 50mg of medlar, 40mg of Chinese date, 100mg of ginseng, 30mg of donkey-hide gelatin, 70mg of collagen, 10mg of cubilose peptide, 10mg of royal jelly freeze-dried powder, 80mg of acerola powder, 10mg of rose powder with multiple petals, 210mg of sorbitol, 132.5mg of D-mannitol and 7.5mg of magnesium stearate.

A tablet for tonifying qi and beautifying face comprises the following steps:

crushing acerola cherry dry matter, sieving with a 40-60-mesh sieve, spraying aqueous solution of papain, spraying the aqueous solution of papain with the spraying amount of 10 percent of the total mass of the powder, performing enzymolysis at 37-40 ℃ for 4-6 h, performing steam blanching for 5min (90 +/-0.5 ℃), and mixing with Chinese wolfberry dry matter, chinese date dry matter, ginseng dry matter and rose dry matter;

step two, taking the cubilose peptide and water, uniformly mixing the cubilose peptide and the water according to the mass ratio of 1:5, performing vacuum low-temperature spray drying after microwave radiation, wherein the spray drying parameters are as follows: the inlet air temperature is 120 ℃, the outlet temperature is 90 ℃, the inlet air flow is 5m ³ /min；

And step three, mixing the powder obtained in the step one and the powder obtained in the step two, adding donkey-hide gelatin powder, collagen powder, royal jelly freeze-dried powder, sorbitol, D-mannitol and magnesium stearate, uniformly mixing, and directly tabletting, wherein the weight of the tablet is 0.75g, and the tablet is punched by a single punch tablet machine with a 6mm shallow recess.

Wherein the enzyme preparation is 10% papain aqueous solution.

Microwave radiation: the frequency can be controlled between 300 MHz and 500MHz

Test example 2

This test example is different from test example 1 only in that the bird's nest peptide was not subjected to microwave irradiation and spray drying treatment.

Test example 3

This test example differs from test example 1 only in that magnesium stearate is not contained.

Example 3 compression testing

The application discovers that the compressibility of the acerola cherry powder is poor and the layering problem is easy to occur when the acerola cherry powder is used for tabletting alone, the compressibility of the acerola cherry powder is slightly improved when the acerola cherry powder is mixed with Chinese wolfberry dry matter, chinese date dry matter, ginseng dry matter and double-petal rose dry matter, but the tabletting yield is still to be improved. However, the composition was directly tabletted and a sticking problem was found to occur.

The compositions of test examples 1 to 3 were tabletted, and observed for delamination and sticking, and tablet hardness was measured using a tablet hardness tester.

TABLE 3

According to the actual situation, the hardness is preferably controlled within the range of 35 to 60N. Therefore, the tablet of test example 1 has better properties and no delamination and sticking. In addition, the bird's nest peptide treated by microwave radiation and spray drying in the comparative test example 1 and the test example 2 can effectively solve the sticking problem in the tabletting process of the composition composed of acerola and other components and improve the hardness of the tablets.

EXAMPLE 5 anti-aging efficacy test

The compositions of test examples 1 to 2 were subjected to an anti-aging efficacy test. 20 volunteers with skin fine lines of 30-50 years of age are selected. The compositions of test examples 1 to 2 were eaten in 2 groups. The food is taken once a day, one granule at a time, and the eating period is two weeks. Before eating, the facial skin is subjected to data acquisition and facial image photographing analysis, and the data is used as a skin initial value. The change in the value of skin elasticity was measured with a skin elasticity tester. Each data is tested three times, and after the test data is averaged, two bits behind the decimal point are reserved. The test results are shown in table 4.

TABLE 4

Test examples	Increase in skin elasticity (%)
		Test example 1	21.70
Test example 2	14.65

As can be seen from the results of Table 4, the composition of test example 1 had a superior anti-aging effect and was able to restore skin elasticity.

Example 6 whitening efficacy test

The compositions of test examples 1 to 2 were subjected to a whitening efficacy test. 10 test subjects, age 30-40, were selected and divided into 2 groups, each of which was a test group consuming the compositions of test examples 1-2. An ultraviolet-induced human skin blackening model whitening efficacy evaluation method is used, a xenon arc lamp sunlight simulator (290-400 nm) which has continuous spectrum radiation and can generate UVA + UVB wavelength ultraviolet rays is adopted, and 2 areas of skin (the same area and the same area) on the back are marked for carrying out an irradiation experiment. The percent reduction in melanin on days 7, 14, 21, and 28 was detected and recorded clinically using a skin color instrument. The test results are shown in table 5.

TABLE 5

Test examples	Percentage of melanin reduction (%)
		Test example 1	19.40
Test example 2	8.34

As can be seen from the results of table 5, the composition of test example 1 had better whitening efficacy.

Example 7 efficacy test for invigorating Qi

30 healthy mice were selected and randomly divided into three groups. The specific construction method for constructing the blood loss blood deficiency mouse model comprises the following steps: the tail tip of the mouse is cut off by about 0.3cm, the wound is immersed in warm water at about 37 ℃, about 0.5 ml of blood is discharged by lightly stroking fingers, and the blood deficiency mouse is obtained after 24 hours of blood discharge. Three groups of blood-deficient mice were fed the composition of test example 1, a commercially available product for invigorating qi, and water, one dose a day. The mice were continuously observed for two weeks for whether the hair had luster, whether the back of the human body was arched, whether the tail was pale in color, and whether abnormal feces were present, and the specific observation results are shown in table 6.

TABLE 6

Test examples	Case of quilt fur	Situation of arch and back	Tail color	Condition of stool
					Test example 1	Has luster	Without bow back	Ruddy complexion	Is normal and normal
Control group	Has luster	Without bow back	Ruddy complexion	Occasional abnormality
					Blank control group	Dull luster	Bow back	Pale with reddish brown	Abnormality (S)

As can be seen from the results in Table 6, the composition of test example 1 of this application has the effects of invigorating qi and replenishing blood.

The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement or the like made within the spirit and principle of the present application shall be included in the scope of the claims of the present application.

Claims (10)

1. The composition for tonifying qi and beautifying is characterized by comprising, by mass, 10-15% of acerola cherry, 1-5% of cubilose peptide, 5-10% of medlar, 1-10% of Chinese date, 10-20% of ginseng, 1-5% of donkey-hide gelatin, 5-10% of collagen, 1-5% of royal jelly and 1-3% of rose with double petals.

2. The qi-tonifying and beauty-maintaining composition according to claim 1, wherein the composition comprises, by mass, 10.7% acerola cherry, 1.33% cubilose peptide, 6.67% medlar, 5.33% jujube, 13.3% ginseng, 4% donkey-hide gelatin, 9.33% collagen, 1.33% royal jelly, and 1.33% rose.

3. The qi-tonifying and beauty-maintaining composition according to claim 1, further comprising a sweetener, wherein the sweetener is one or more of sorbitol, D-mannitol, erythritol and lactitol; preferably, sorbitol accounting for 25 to 30% of the total mass, 15 to 20% of D-mannitol; more preferably, 28% sorbitol, 17.7% D-mannitol.

4. The qi invigorating and skin caring composition according to claim 1, further comprising an auxiliary agent, wherein the auxiliary agent is 1% magnesium stearate.

5. A method for preparing the composition for invigorating qi and caring skin according to any one of claims 1-4, comprising the steps of:

step one, grinding acerola cherry to 40-60 meshes, spraying an enzyme preparation, reacting at 37-40 ℃ for 4-6 h, steam blanching at 80-95 ℃ for 4-6 min, and mixing with medlar, chinese date, ginseng and rose to obtain first powder;

step two, dissolving the cubilose peptide in water, uniformly mixing, performing microwave radiation, and performing vacuum low-temperature spray drying to obtain second powder;

and step three, mixing the first powder and the second powder, adding donkey-hide gelatin, collagen, royal jelly, sorbitol, D-mannitol and magnesium stearate, and uniformly mixing to obtain the composition.

6. The method according to claim 5, characterized in that the enzyme preparation is an aqueous solution of papain at a concentration of 10-15%, preferably 10%;

the addition amount of the enzyme preparation is 10 percent of that of the acerola cherry in percentage by mass.

7. The method according to claim 5, wherein the mass ratio of the cubilose peptide to the water is 1: (4-5); preferably, 1:5.

8. The method of claim 5, wherein the spray drying parameters are: the inlet air temperature is 100-120 ℃, the outlet temperature is 80-90 ℃, the inlet air flow is 4-5 m ³ Min; preferably, the inlet air temperature is 120 ℃, the outlet temperature is 90 ℃, the inlet air flow is 5m ³ /min。

9. A health food comprising the composition according to any one of claims 1 to 4;

preferably, the dosage form of the health food is tablets, capsules or granules; more preferably, a tablet.

10. The use of the health food of claim 9 for invigorating qi and beautifying.