CN115629213B - Poultry virus antigen detection card - Google Patents

Poultry virus antigen detection card Download PDF

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Publication number
CN115629213B
CN115629213B CN202211653266.7A CN202211653266A CN115629213B CN 115629213 B CN115629213 B CN 115629213B CN 202211653266 A CN202211653266 A CN 202211653266A CN 115629213 B CN115629213 B CN 115629213B
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China
Prior art keywords
pipe
sampling
sliding
fixedly connected
device pipe
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CN202211653266.7A
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CN115629213A (en
Inventor
刘增再
张韬
薛爽
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Changsha Animal And Plant Disease Prevention And Control Center
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Changsha Animal And Plant Disease Prevention And Control Center
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a poultry virus antigen detection card, which relates to the field of poultry leukemia virus antigen detection and solves the problems that the existing poultry virus antigen detection card is scattered in structure, complex in operation and affects sampling efficiency and accuracy of detection results when in use.

Description

Poultry virus antigen detection card
Technical Field
The invention relates to the technical field of avian leukosis virus antigen detection, in particular to a poultry virus antigen detection card.
Background
Avian leukemia is a collective term for various neoplastic diseases of the birds caused by viruses of the avian C-type retrovirus group, as well as for infectious diseases and parasitic diseases. Mainly lymphoblastic leukemia, and secondly erythroblastic leukemia and myeloblastic leukemia. In addition, it can cause myeloma, connective tissue tumor, epithelial tumor, endothelial tumor, etc. Most tumors invade the hematopoietic system, and a few invade other tissues such as egg drop, immunosuppression, etc.
Because the transmission mode of the avian leukosis is mostly vertical transmission, the treatment cost of the disease is higher in the current medicine, in order to avoid the transmission of the virus, the breeding poultry is generally detected, the positive poultry is eliminated, the population is optimized, and the screening of the virus is realized. When detecting antigens, the conventional poultry virus antigen detection card needs to be inserted into the vagina of poultry for sampling when detecting the vagina swab, and then the cotton swab is put into the extracting solution and dripped on the detection card after sampling is finished, and whether the poultry has poultry leukemia can be judged by observing the number of the cross bars on the detection card. To this end, we propose a poultry virus antigen detection card.
Disclosure of Invention
The invention aims to provide a poultry virus antigen detection card which is convenient for realizing rapid sampling detection and reducing detection interference at the same time, so as to solve the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: the utility model provides a poultry virus antigen detects card, includes casing, sampling mechanism, detection mechanism and mixed mechanism, it has lower casing to peg graft on the casing to go up, lower casing with the joint has the connecting pipe between the casing, the one end fixedly connected with device pipe of connecting pipe, sampling mechanism including install in the intraductal sampling stick of device, the one end fixedly connected with cotton swab of sampling stick, set up in the intraductal storage chamber that is used for storing the extract that has offered of device, sampling mechanism is used for controlling during the sample sampling stick drives the cotton swab stretches out from in the device pipe and takes a sample, and the linkage will during the withdrawal in the intraductal extraction liquid of device discharges into the position of cotton swab carries out the sample and draws, detection mechanism install in the inferior valve for detect the poultry leukemia virus antigen in the extraction liquid, mixed mechanism install in the intraductal for control the cotton swab is arranged in the extraction liquid after stirring the setting up the number of times in the extraction liquid and is detected in the detection mechanism, is convenient for realize the quick interference detection that reduces simultaneously.
Preferably, the sampling mechanism comprises a threaded pipe fixedly mounted at one end of the device pipe, a threaded cover is connected to the threaded pipe in a threaded manner, a sliding groove and a mixing cavity are formed in the device pipe, the cotton swab is located in the mixing cavity, the sampling rod is slidably connected with the inner wall of the sliding groove, a control piece for controlling the cotton swab to conduct telescopic sampling is arranged in the device pipe, a linkage piece for discharging the extracting solution in the storage cavity into the mixing cavity after the cotton swab completes one-time telescopic sampling is arranged in the device pipe, rapid sampling and retracting operation are conveniently achieved, and external interference is reduced.
Preferably, the control member comprises a driving rod fixedly mounted on the sampling rod, a guide groove is formed in the upper shell, a control block fixedly connected with one end of the driving rod is slidably connected in the guide groove, a communication groove is formed in the device tube, and the driving rod is slidably connected with the communication groove, so that the cotton swab can be conveniently controlled to be subjected to telescopic sampling.
Preferably, the linkage piece comprises a rotating column rotationally connected with the mixing cavity, a communication hole communicated with the mixing cavity is formed in the rotating column, a sliding tube is slidably connected in the communication groove, a control tube used for communicating the storage cavity with the communication hole is arranged in the sliding tube, a first sliding groove is formed in the device tube, a first clamping block is slidably connected in the first sliding groove, a clamping groove clamped with the first clamping block is formed in the side face of the rotating column, and the linkage is convenient for discharging extracting solution in the storage cavity into the mixing cavity after the cotton swab is subjected to one-time telescopic sampling.
Preferably, the mixing mechanism comprises a push rod fixedly mounted on the sampling rod, a first pipeline communicated with the connecting pipe is arranged in the sampling rod, a second pipeline used for communicating the first pipeline with the communicating hole is arranged on the side face of the sampling rod, a sleeved pipe movably sleeved on the outer wall of the sampling rod is fixedly connected with the side face of the rotating column, a mixing piece used for pushing and pulling the control block back and forth after sampling of the cotton swab is completed is arranged in the device pipe, so that the cotton swab and the extracting solution are mixed in the process of linkage, the rotating column rotates in one direction, and the cotton swab and the extracting solution after sampling are convenient to fully contact.
Preferably, the mixing element comprises a first bevel gear coaxially and fixedly arranged on one side of the rotating column, a second bevel gear meshed with the first bevel gear is rotationally connected in the device tube, a second sliding groove is formed in the device tube, a second clamping block clamped with the clamping groove is slidably connected in the second sliding groove, a first spring fixedly connected with the second sliding groove is fixedly connected to the second clamping block, and a driving element for driving the second bevel gear to rotate unidirectionally is arranged on the pushing rod, so that the cotton swab is convenient to rotate unidirectionally in linkage with the rotating column in the process of mixing the cotton swab with the extracting solution by pushing and pulling the control block back and forth after sampling of the cotton swab is completed.
Preferably, the driving piece comprises a rack which is in sliding connection with the device pipe, one end of the rack is fixedly connected with a second spring which is fixedly connected with the pushing rod, a rotating disc is coaxially and fixedly connected with the second bevel gear, a plurality of groups of arc plates are uniformly and rotatably connected to the side face of the rotating disc through a spring rotating shaft, and the second bevel gear is convenient to drive to rotate unidirectionally.
Preferably, the detection mechanism comprises a sample pad fixedly mounted in the lower shell, one side of the sample pad is attached to one end of the connecting pipe, one end of the sample pad is fixedly connected with colloidal gold, one end of the colloidal gold is fixedly connected with an NC film, a test wire and a control wire are arranged on the NC film, one end of the NC film is fixedly connected with water-absorbing filter paper, and a transparent plastic plate for observing the test wire and the control wire is fixedly connected to the upper shell, so that the detection of avian leukosis virus antigens in the extracting solution is facilitated.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the poultry virus antigen detection card provided by the invention, the sampling rod and the cotton swab are pushed out by pushing the control block to drive the driving rod, the cotton swab head can be inserted into the position where sampling is needed for poultry to sample, after sampling is completed, the cotton swab is driven by the back-pull control block to be recovered into the mixing cavity, sampling can be completed by covering the threaded cover, meanwhile, in the process of pushing the driving rod, the driving rod can slide in the communicating groove, one end of the sliding tube is pushed by the top end to slide in the communicating groove, so that the communication between the storage cavity and the communicating hole is realized, the extracting solution can be discharged into the mixing cavity through the control tube and the communicating hole, one end of the threaded cover can be downwards poured out of the extracting solution in the storage cavity to be mixed in the mixing cavity, the purpose of sampling is realized, and meanwhile, a plurality of devices are not required to be mutually switched, only the control block is needed to be pushed and pulled, and the operation is more convenient and efficient.
2. According to the poultry virus antigen detection card provided by the invention, the driving rod and the sampling rod are driven to move by continuously pushing and pulling the control block, so that the cotton swab is fully mixed with the extracting solution in the mixing cavity, the sampling rod drives the pushing rod to slide to continuously push the second spring and the rack to slide in the device pipe, the rotating disc and the second bevel gear are driven to rotate in one direction, the first bevel gear and the rotating column are driven to rotate, the communication hole is gradually rotated to release the connection relation between the communication hole and the control pipe when the rotating column rotates, the second clamping block is clamped into the clamping groove after the rotating column rotates by a set angle, the rotating column is not rotated any more, the mixing cavity is communicated with the second pipeline and the first pipeline, the extracting solution after the mixing is completely discharged into the connecting pipe through the communication hole and is absorbed by the sample pad through the inversion device pipe, and the detection result can be obtained through the test wire and the control wire on the NC film after the marking of colloidal gold.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the structure of the detecting mechanism of the present invention;
FIG. 3 is an exploded view of the overall structure of the present invention;
FIG. 4 is an enlarged view of area A of FIG. 3;
FIG. 5 is a schematic diagram of a sampling mechanism according to the present invention;
FIG. 6 is a schematic view of the internal structure of the present invention;
FIG. 7 is an enlarged view of area B of FIG. 6;
FIG. 8 is a schematic diagram of a mixing mechanism according to the present invention;
FIG. 9 is an enlarged view of region C of FIG. 8;
FIG. 10 is a cross-sectional view of the tube structure of the device of the present invention;
FIG. 11 is a cross-sectional view of the sampling mechanism of the present invention.
In the figure: 1-an upper housing; 2-a lower housing; 3-connecting pipes; 4-a device tube; 5-a sampling mechanism; 6-sampling bar; 7-cotton swabs; 8-a storage chamber; 9-a detection mechanism; 10-a mixing mechanism; 11-threaded pipe; 12-a threaded cap; 13-a sliding groove; 14-a mixing chamber; 15-a control member; 16-linkage; 17-a drive rod; 18-a guide groove; 19-a control block; 20-a communicating groove; 21-a rotating column; 22-communicating holes; 23-sliding tube; 24-control tube; 25-a first chute; 26-a first clamping block; 27-clamping grooves; 28-push rod; 29-a first conduit; 30-a second conduit; 31-a ferrule; 32-mixing element; 33-a first bevel gear; 34-a second bevel gear; 35-a second chute; 36-a second clamping block; 37-a first spring; 38-a driving member; 39-rack; 40-a second spring; 41-rotating a disc; 42-arc plate; 43-sample pad; 44-colloidal gold; 45-NC film; 46-test lines; 47-control line; 48-water absorption filter paper; 49-transparent plastic plate.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-11, the present invention provides a technical solution: the utility model provides a poultry virus antigen detects card, including last casing 1, sampling mechanism 5, detection mechanism 9 and mixed mechanism 10, go up and peg graft on the casing 1 and have lower casing 2, lower casing 2 is made for degradable plastics material with last casing 1, the joint has connecting pipe 3 between lower casing 2 and the last casing 1, install through the joint prevention, be convenient for production and cooperation installation between the part, the one end fixedly connected with device pipe 4 of connecting pipe 3, sampling mechanism 5 is including installing the sampling stick 6 in device pipe 4, the one end fixedly connected with cotton swab 7 of sampling stick 6, set up the storage chamber 8 that is used for storing the extract in the device pipe 4, sampling mechanism 5 is used for controlling sampling stick 6 to drive cotton swab 7 and stretch out from device pipe 4 and take a sample when retrieving device pipe 4 in the position that the extract in the storage chamber 8 was discharged into cotton swab 7, detection mechanism 9 installs in lower casing 2, be used for detecting leukemia virus antigen in the extract, mixed mechanism 10 installs in device pipe 4, be used for controlling stirring in the extract and detect the number of times of setting up in the detection mechanism after extracting liquid.
The sampling mechanism 5 comprises a threaded pipe 11 fixedly arranged at one end of the device pipe 4, a threaded cover 12 is connected to the threaded pipe 11 in a threaded manner, a sliding groove 13 and a mixing cavity 14 are formed in the device pipe 4, the cotton swab 7 is located in the mixing cavity 14, the sampling rod 6 is slidably connected with the inner wall of the sliding groove 13, a control piece 15 for controlling the cotton swab 7 to conduct telescopic sampling is arranged in the device pipe 4, a linkage piece 16 for discharging the extracting solution in the storage cavity 8 into the mixing cavity 14 in linkage manner after the cotton swab 7 completes one-time telescopic sampling is arranged in the device pipe 4, the control piece 15 comprises a driving rod 17 fixedly arranged on the sampling rod 6, a guide groove 18 is formed in the upper shell 1, a control block 19 fixedly connected with one end of the driving rod 17 is slidably connected in the guide groove 18, a communicating groove 20 is formed in the device pipe 4, and the driving rod 17 is slidably connected with the communicating groove 20.
The linkage piece 16 comprises a rotating column 21 rotationally connected with the mixing cavity 14, a communication hole 22 communicated with the mixing cavity 14 is formed in the rotating column 21, a sliding tube 23 is slidably connected in the communication groove 20, a control tube 24 used for communicating the storage cavity 8 with the communication hole 22 is formed in the sliding tube 23, a first sliding groove 25 is formed in the device tube 4, a first clamping block 26 is slidably connected in the first sliding groove 25, and a clamping groove 27 clamped with the first clamping block 26 is formed in the side face of the rotating column 21.
The mixing mechanism 10 comprises a push rod 28 fixedly arranged on the sampling rod 6, a first pipeline 29 communicated with the connecting pipe 3 is arranged in the sampling rod 6, a second pipeline 30 used for communicating the first pipeline 29 with the communicating hole 22 is arranged on the side surface of the sampling rod 6, a sleeved pipe 31 movably sleeved with the outer wall of the sampling rod 6 is fixedly connected to the side surface of the rotating column 21, and a mixing piece 32 used for carrying out unidirectional rotation on the linkage rotating column 21 in the process of reciprocating push-pull control block 19 after sampling of the cotton swab 7 is completed so as to enable the cotton swab 7 to be mixed with the extracting solution is arranged in the device pipe 4.
The mixing element 32 comprises a first bevel gear 33 coaxially and fixedly arranged on one side of the rotating column 21, a second bevel gear 34 meshed with the first bevel gear 33 is rotationally connected in the device tube 4, a second sliding groove 35 is formed in the device tube 4, a second clamping block 36 clamped with the clamping groove 27 is slidably connected in the second sliding groove 35, a first spring 37 fixedly connected with the second sliding groove 35 is fixedly connected on the second clamping block 36, a driving element 38 for driving the second bevel gear 34 to rotate unidirectionally is arranged on the pushing rod 28, the driving element 38 comprises a rack 39 slidably connected with the device tube 4, a second spring 40 fixedly connected with the pushing rod 28 is fixedly connected at one end of the rack 39, a rotating disc 41 is coaxially and fixedly connected with the second bevel gear 34, and a plurality of groups of arc plates 42 are uniformly and rotationally connected on the side surface of the rotating disc 41 through a rotating shaft.
The detection mechanism 9 comprises a sample pad 43 fixedly arranged in the lower shell 2, one side of the sample pad 43 is attached to one end of the connecting pipe 3, one end of the sample pad 43 is fixedly connected with a colloidal gold 44, one end of the colloidal gold 44 is fixedly connected with an NC film 45, a test wire 46 and a control wire 47 are arranged on the NC film 45, one end of the NC film 45 is fixedly connected with a water-absorbing filter paper 48, and a transparent plastic plate 49 for observing the test wire 46 and the control wire 47 is fixedly connected on the upper shell 1.
Through opening screw cap 12, promote control block 19 and drive actuating lever 17 and release sampling stick 6 and cotton swab 7, can insert the cotton swab 7 head into the required position of taking a sample of poultry and take a sample, back pull control block 19 drives cotton swab 7 and withdraws into mixing chamber 14 after the sample is accomplished, cover screw cap 12 and can accomplish the sample, simultaneously can make actuating lever 17 slide in intercommunication groove 20 in the in-process of promoting actuating lever 17, the one end that the top promoted sliding tube 23 makes sliding tube 23 slide in intercommunication groove 20, the one end of control tube 24 moves to intercommunicating pore 22 gradually, sliding tube 23 promotes first fixture block 26 and slides into first spout 25 gradually and releases the joint to joint groove 27, until actuating lever 17 is after release cotton swab 7 is completely, at this moment control tube 24 realizes the intercommunication to reservoir chamber 8 and intercommunicating pore 22, can be discharged into mixing chamber 14 through control tube 24 and intercommunicating pore 22, because cotton swab 7 top at this moment, the extracting solution can not upwards flow into mixing chamber 14 in the course of pushing actuating lever 17, simultaneously when screw cap 12 closes, sliding distance of actuating lever 17 makes sliding tube 23 slide in intercommunication groove 20 in the mixing chamber 14, can be accomplished in the high order to realize that the mixing chamber 12 is just need to be moved down by the high-efficient, the device is realized in the mixing chamber 12, the mixing chamber is realized in the mixing chamber is only after the end is closed, the device is sealed by the mixing chamber 12 is replaced by the device is not need to be moved, and can be well 8, and can be accomplished by the device is sealed by the device is high-efficient.
The continuous push-pull control block 19 drives the driving rod 17 and the sampling rod 6 to move, so that when the cotton swab 7 is fully mixed with the extracting solution in the mixing cavity 14, the sampling rod 6 can drive the pushing rod 28 to slide to continuously push the second spring 40 and the rack 39 to slide in the device tube 4, thereby driving the rotating disc 41 and the second bevel gear 34 to rotate unidirectionally, when the rack 39 moves forwards, the arc plate 42 can be pushed to the side of the rotating disc 41, at the moment, the rotating disc 41 cannot be driven to rotate, when the control block 19 drives the rack 39 to pull back, the arc plate 42 can be driven to outwards to be meshed with the rack 39 under the action of a spring (the spring for driving the rotating shaft to rotate) in the spiral spring shaft, so that the arc plate 42 is driven to drive the rotating disc 41 and the second bevel gear 34 to rotate under the action of the rack 39, and the first bevel gear 33 and the rotating column 21 can be driven to rotate, the second spring 40 is arranged to give a certain buffer space when the rack 39 moves forwards, so that the sampling rod 6 needs to be pushed out a longer distance when the sampling rod is sampled, and meanwhile, the short distance of the rack 39 is driven to slide when the back mixing shaft is driven, and the reciprocating distance of the rack 39 cannot influence the arc plate 42.
When the rotating column 21 rotates, the connecting hole 22 gradually rotates to release the connection relation with the control tube 24, the rotating column 21 rotates by a set angle to enable the second clamping block 36 to be clamped into the clamping groove 27, at the moment, the rotating column 21 does not rotate continuously, meanwhile, the connecting hole 22 is communicated with the mixed cavity 14 and the second pipeline 30 and the first pipeline 29, the inverted device tube 4 can discharge the mixed extracting solution into the connecting tube 3 through the connecting hole 22 and is absorbed by the sample pad 43, the detection result can be obtained on the NC film 45 through the test wire 46 and the control wire 47 after the marking of the colloidal gold 44, the water-absorbing filter paper 48 can absorb the redundant extracting solution, the transparent plastic plate 49 is used for sealing the NC film 45 to prevent the detection result from being influenced by external factors, meanwhile, after the sampling detection is finished, the whole inside of the device is in a sealing state, the risk of virus propagation is reduced, the control wire 47 is used for judging whether the extracting solution reaches a required position, the test wire 46 is used for judging whether the sample contains virus antigen of avian leukosis or not, meanwhile, the sleeve 31 is effectively sealed to the second pipeline 30 in the sliding process, so that the detecting result can be directly connected into the connecting tube 3 after the sample is set up, the detecting the mixing solution can be directly and the mixed solution is not fully detected through the setting the position of the NC film 45.
It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (2)

1. A poultry virus antigen detection card comprising:
the device comprises an upper shell (1), wherein a lower shell (2) is inserted on the upper shell (1), a connecting pipe (3) is clamped between the lower shell (2) and the upper shell (1), and one end of the connecting pipe (3) is fixedly connected with a device pipe (4);
further comprises:
the sampling mechanism (5), sampling mechanism (5) including install in sampling stick (6) in device pipe (4), the one end fixedly connected with cotton swab (7) of sampling stick (6), set up in device pipe (4) and be used for storing extraction liquid's storage chamber (8), sampling mechanism (5) are used for controlling when taking a sample sampling stick (6) drive cotton swab (7) stretches out in device pipe (4) and take a sample, and when retrieving in device pipe (4) the linkage with the extraction liquid in storage chamber (8) is discharged into the position of cotton swab (7) and is taken a sample;
the detection mechanism (9) is arranged in the lower shell (2) and is used for detecting avian leukosis virus antigens in the extracting solution;
mixing mechanism (10), mixing mechanism (10) install in device pipe (4), be used for controlling in the extracting solution stirs in the extracting solution and set up the number of times back with extracting solution row into detect mechanism (9) in detect, sampling mechanism (5) including fixed mounting in screw thread pipe (11) of device pipe (4) one end, screw thread connection has screw thread lid (12) on screw thread pipe (11), set up sliding tray (13) and hybrid chamber (14) in device pipe (4), cotton swab (7) are located hybrid chamber (14), sampling rod (6) with sliding tray (13) inner wall sliding connection, be equipped with in device pipe (4) be used for controlling cotton swab (7) carry out flexible control piece (15) of sampling, be equipped with in device pipe (4) be used for in cotton swab (7) accomplish flexible after the sampling linkage with extracting solution row in reservoir (8) in hybrid chamber (14) link piece (16), cotton swab (7) are located hybrid chamber (14) inner wall sliding tray (6) is located in hybrid chamber (14), sliding tray (6) inner wall sliding tray (17) is equipped with on fixed connection (17) one end, on control piece (17) are connected with one end (17), the device comprises a device pipe (4), a communicating groove (20) is formed in the device pipe (4), a driving rod (17) is in sliding connection with the communicating groove (20), a rotating column (21) rotationally connected with a mixing cavity (14) is arranged on a linkage piece (16), a communicating hole (22) communicated with the mixing cavity (14) is formed in the rotating column (21), a sliding pipe (23) is connected in the communicating groove (20) in a sliding manner, a control pipe (24) used for communicating a storage cavity (8) with the communicating hole (22) is formed in the sliding pipe (23), a first sliding groove (25) is formed in the device pipe (4), a first clamping block (26) is connected in the sliding connection of the first sliding groove (25), a clamping groove (27) clamped with the first clamping block (26) is formed in the side face of the rotating column (21), a pushing rod (28) fixedly arranged on the sampling rod (6), a first connecting pipe (29) is formed in the sampling rod (6), a first connecting pipe (29) is communicated with the first side face (6), the side of the rotating column (21) is fixedly connected with a sleeve pipe (31) movably sleeved on the outer wall of the sampling rod (6), a second bevel gear (34) meshed with the first bevel gear (33) is arranged in the device pipe (4), a second sliding groove (35) is arranged in the device pipe (4), a second clamping block (36) clamped with the clamping groove (27) is arranged in the second sliding groove (35) in a sliding manner, a first spring (37) fixedly connected with the second sliding groove (35) is fixedly connected to the second clamping block (36), the first bevel gear (33) is coaxially and fixedly arranged on the mixing member (32), a second bevel gear (34) meshed with the first bevel gear (33) is rotatably connected to the device pipe (4), a second sliding groove (35) is arranged in the device pipe (4), a second clamping block (36) is connected to the second clamping groove (27) in a sliding manner, a first pushing rod (37) fixedly connected to the second sliding groove (35), a driving rod (28) is arranged on the pushing rod (28), the driving rod (38) is connected to the second sliding rod (38) and comprises a first driving rod (38), the second bevel gear (34) is fixedly connected with a rotating disc (41) coaxially, and a plurality of groups of arc plates (42) are uniformly and rotatably connected to the side surface of the rotating disc (41) through a spring rotating shaft.
2. A poultry virus antigen detection card according to claim 1, wherein: the detection mechanism (9) comprises a sample pad (43) fixedly mounted in the lower shell (2), one side of the sample pad (43) is attached to one end of the connecting pipe (3), one end of the sample pad (43) is fixedly connected with colloidal gold (44), one end of the colloidal gold (44) is fixedly connected with an NC film (45), a test wire (46) and a control wire (47) are arranged on the NC film (45), one end of the NC film (45) is fixedly connected with water-absorbing filter paper (48), and a transparent plastic plate (49) used for observing the test wire (46) and the control wire (47) is fixedly connected to the upper shell (1).
CN202211653266.7A 2022-12-22 2022-12-22 Poultry virus antigen detection card Active CN115629213B (en)

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Application Number Priority Date Filing Date Title
CN202211653266.7A CN115629213B (en) 2022-12-22 2022-12-22 Poultry virus antigen detection card

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Application Number Priority Date Filing Date Title
CN202211653266.7A CN115629213B (en) 2022-12-22 2022-12-22 Poultry virus antigen detection card

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CN115629213A CN115629213A (en) 2023-01-20
CN115629213B true CN115629213B (en) 2023-05-09

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